Spg031386 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg031386 vs. NCBI nr
Match: XP_038893357.1 (transcription factor 25 [Benincasa hispida])

HSP 1 Score: 1065.1 bits (2753), Expect = 2.4e-307
Identity = 532/589 (90.32%), Postives = 557/589 (94.57%), Query Frame = 0

Query: 48  DEDDLGTHLEDKDNDPEAADEISVDMHKQFVRNGAKSAISTSNKKLKKKKKKKERENSSS 107
           D++D       +DND EA DEISVDMHKQFVRNG KSAISTSNKKLKKKKKKKERENSSS
Sbjct: 50  DDEDDSQISPQQDNDQEAVDEISVDMHKQFVRNGTKSAISTSNKKLKKKKKKKERENSSS 109

Query: 108 TTVTDKILDKPLDLILESLSLDVKPSASSRQQGPEKAKSGAEERNCGKQCLPSLLEVDPK 167
           T  TDKILDKPLD++LESLSLD  PSA S  QGPEKAK+GAEERNCGKQ +PSLLEVDPK
Sbjct: 110 T--TDKILDKPLDVLLESLSLDTIPSALSSLQGPEKAKNGAEERNCGKQRMPSLLEVDPK 169

Query: 168 YLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHLARRTYLVSPSDHWPRWDGS 227
           YLNAGNELRRIFGSKVVKSFEKNNQASSSRQ+RGGRRVNHL+R+TYLVSPSDHWPRWDGS
Sbjct: 170 YLNAGNELRRIFGSKVVKSFEKNNQASSSRQVRGGRRVNHLSRKTYLVSPSDHWPRWDGS 229

Query: 228 ISMEFLETRNGYHYFRYVYPPSYVEAQKAFEDAKSIHDLNGIASILLYHPYHLDSLITMA 287
           +SMEFLETRNGYHYFRYV+PPSYVEAQKA+E A+SIHDLNGIASILL+HPYHLDSLITMA
Sbjct: 230 LSMEFLETRNGYHYFRYVHPPSYVEAQKAYEAARSIHDLNGIASILLHHPYHLDSLITMA 289

Query: 288 EYFKFVGDHEMSSDAVAKSLYALECAWHPMFTVLQGNCQLKISHETNKPMFTSLFTHVKY 347
           EYFKF GDHEMSSDAVAKSLYALECAWHPMFTV QGNCQLKI HETNKPMFT LFTHVK 
Sbjct: 290 EYFKFAGDHEMSSDAVAKSLYALECAWHPMFTVFQGNCQLKIRHETNKPMFTLLFTHVKN 349

Query: 348 LDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYKNDTSLWL 407
           LDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRA+EYTWLERF ESYKNDTSLWL
Sbjct: 350 LDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAQEYTWLERFGESYKNDTSLWL 409

Query: 408 FPNFSFSLSICRFYLEREEASKDDNVDATRANSRDLLKQALMLHPLVLKRLVEKVPLKEQ 467
           FPNFSFSLSICRFYLEREE SK DN+DATRANS DLLKQALMLHPLVLKR+VEKVPLKEQ
Sbjct: 410 FPNFSFSLSICRFYLEREEPSKVDNLDATRANSSDLLKQALMLHPLVLKRIVEKVPLKEQ 469

Query: 468 FWVHILKHSFFLPDQTGIPSLDHLINLYVERNYIIWRIPDLQKLLRETAQSVIEVLETDR 527
           FWVHILKHSFFLPDQTGIPSLDHLINLYVERNYIIWRIPDLQK LRETAQ VIE LETD+
Sbjct: 470 FWVHILKHSFFLPDQTGIPSLDHLINLYVERNYIIWRIPDLQKFLRETAQLVIEALETDQ 529

Query: 528 NDAKDWACVRKEAFSSEKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQHV 587
           NDAKDWACVRKEAFSSEKNEYAHLLVSDFSDS+S+APPEALQDFVVDPRMR+VQNIIQH 
Sbjct: 530 NDAKDWACVRKEAFSSEKNEYAHLLVSDFSDSLSTAPPEALQDFVVDPRMRNVQNIIQHA 589

Query: 588 NPPDQRQPTREIANRSALAVLFESMLPWVNYGDERDVGVDEGNQFDEHD 637
           NPPD++QPT+EIANRSALAVLFESMLPWVNYGDERDVGVDEGNQFDEHD
Sbjct: 590 NPPDRQQPTQEIANRSALAVLFESMLPWVNYGDERDVGVDEGNQFDEHD 636

BLAST of Spg031386 vs. NCBI nr
Match: XP_022935196.1 (transcription factor 25 [Cucurbita moschata])

HSP 1 Score: 1058.5 bits (2736), Expect = 2.2e-305
Identity = 535/591 (90.52%), Postives = 559/591 (94.59%), Query Frame = 0

Query: 48  DEDDLGTHLEDKDNDPEAADEISVDMHKQFVRNGAKSAISTSNKKLKKKKKKKERENSSS 107
           DEDD   +   +D+D EAA+E+SV+MHK+FVRNG KSA STSNKKLKKKKKKKERENSSS
Sbjct: 53  DEDDSQIN-PQQDDDQEAAEEVSVNMHKEFVRNGTKSASSTSNKKLKKKKKKKERENSSS 112

Query: 108 TTVTDKILDKPLDLILESLSLDVKPSASSRQQGPEKAKSGA-EERNCGKQCLPSLLEVDP 167
           T  T+KILDKPLD+ILESLSLDV PSA SRQQ PEKAK    EERN GKQCL SLLEVDP
Sbjct: 113 T--TNKILDKPLDVILESLSLDVNPSALSRQQDPEKAKDVVEEERNRGKQCLTSLLEVDP 172

Query: 168 KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHLARRTYLVSPSDHWPRWDG 227
           KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHL+R+TYLVSPSDHWPRWDG
Sbjct: 173 KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHLSRKTYLVSPSDHWPRWDG 232

Query: 228 SISMEFLETRNGYHYFRYVYPPSYVEAQKAFEDAKSIHDLNGIASILLYHPYHLDSLITM 287
           S+SMEFLET+NGYHYFRYV+PPSYVEAQKAFE AKSIHDLNGIASILL+HPYHLDSLITM
Sbjct: 233 SLSMEFLETKNGYHYFRYVHPPSYVEAQKAFEAAKSIHDLNGIASILLHHPYHLDSLITM 292

Query: 288 AEYFKFVGDHEMSSDAVAKSLYALECAWHPMFTVLQGNCQLKISHETNKPMFTSLFTHVK 347
           AEYFKF GDHEMSSDAVAKSLYALECAWHPMFT LQGNCQLKISHETNKPMFTSLFTHVK
Sbjct: 293 AEYFKFAGDHEMSSDAVAKSLYALECAWHPMFTALQGNCQLKISHETNKPMFTSLFTHVK 352

Query: 348 YLDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYKNDTSLW 407
            LDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYKNDTSLW
Sbjct: 353 NLDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYKNDTSLW 412

Query: 408 LFPNFSFSLSICRFYLEREEASKDDNVDATRANSRDLLKQALMLHPLVLKRLVEKVPLKE 467
           LFPNFSFSLS+CR+YLEREE SK+DN+DATRANS DLLKQALMLHPLVLKRLVEKVPLKE
Sbjct: 413 LFPNFSFSLSVCRYYLEREEDSKNDNLDATRANSTDLLKQALMLHPLVLKRLVEKVPLKE 472

Query: 468 QFWVHILKHSFFLPDQTGIPSLDHLINLYVERNYIIWRIPDLQKLLRETAQSVIEVLETD 527
           QFWVHILK+SFF  DQTGIPSLDHLINLYVERNY+IWRIPDLQKLL+ETAQ VIEVLETD
Sbjct: 473 QFWVHILKNSFFSSDQTGIPSLDHLINLYVERNYLIWRIPDLQKLLKETAQLVIEVLETD 532

Query: 528 RNDAKDWACVRKEAFSSEKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH 587
           RNDAKDWACVRKEAFS EKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH
Sbjct: 533 RNDAKDWACVRKEAFSCEKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH 592

Query: 588 VNPPDQRQPTREIANRSALAVLFESMLPWVNYGDERDVGVDEGNQFDEHDH 638
            NPP+QR PTREIANRSALAVLFESMLPWVNYGDERD+GVDEGNQFDEHDH
Sbjct: 593 ANPPNQRHPTREIANRSALAVLFESMLPWVNYGDERDIGVDEGNQFDEHDH 640

BLAST of Spg031386 vs. NCBI nr
Match: KAG6597002.1 (Transcription factor 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1055.4 bits (2728), Expect = 1.9e-304
Identity = 533/591 (90.19%), Postives = 559/591 (94.59%), Query Frame = 0

Query: 48  DEDDLGTHLEDKDNDPEAADEISVDMHKQFVRNGAKSAISTSNKKLKKKKKKKERENSSS 107
           DEDD   +   +D+D EAA+E+SV+MHK+FVRNG KSA STSNKKLKKKKKKKERENSSS
Sbjct: 53  DEDDSQIN-PQQDDDQEAAEEVSVNMHKEFVRNGTKSASSTSNKKLKKKKKKKERENSSS 112

Query: 108 TTVTDKILDKPLDLILESLSLDVKPSASSRQQGPEKAKSGA-EERNCGKQCLPSLLEVDP 167
           T  T+KILDKPLD+ILESLSLDV PSA SRQQ PEKAK    EERN GKQCL SLLEVDP
Sbjct: 113 T--TNKILDKPLDVILESLSLDVNPSALSRQQDPEKAKDVVEEERNRGKQCLTSLLEVDP 172

Query: 168 KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHLARRTYLVSPSDHWPRWDG 227
           KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHL+R+TYLVSPSDHWPRWDG
Sbjct: 173 KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHLSRKTYLVSPSDHWPRWDG 232

Query: 228 SISMEFLETRNGYHYFRYVYPPSYVEAQKAFEDAKSIHDLNGIASILLYHPYHLDSLITM 287
           S+SMEFLET+NGYHYFRYV+PPSYVEAQKAFE AKSIHDLNGIASILL+HPYHLDSLITM
Sbjct: 233 SLSMEFLETKNGYHYFRYVHPPSYVEAQKAFEAAKSIHDLNGIASILLHHPYHLDSLITM 292

Query: 288 AEYFKFVGDHEMSSDAVAKSLYALECAWHPMFTVLQGNCQLKISHETNKPMFTSLFTHVK 347
           AEYFKF GDHEMSSDAVAKSLYALECAWHPMFT LQGNCQLKISHETNKPMFTSLFTHVK
Sbjct: 293 AEYFKFAGDHEMSSDAVAKSLYALECAWHPMFTALQGNCQLKISHETNKPMFTSLFTHVK 352

Query: 348 YLDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYKNDTSLW 407
            LDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEY+WLERFAESYKNDTSLW
Sbjct: 353 NLDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYSWLERFAESYKNDTSLW 412

Query: 408 LFPNFSFSLSICRFYLEREEASKDDNVDATRANSRDLLKQALMLHPLVLKRLVEKVPLKE 467
           LFPNFSFSLS+CR+YLEREE SK+DN+DATRANS DLLKQALMLHPLVLKRLVEKVPLKE
Sbjct: 413 LFPNFSFSLSVCRYYLEREEDSKNDNLDATRANSTDLLKQALMLHPLVLKRLVEKVPLKE 472

Query: 468 QFWVHILKHSFFLPDQTGIPSLDHLINLYVERNYIIWRIPDLQKLLRETAQSVIEVLETD 527
           QFWVHILK+SFF  DQTGIPSLDHLINLYVERNY+IWRIPDLQKLL+ETAQ VIEVLETD
Sbjct: 473 QFWVHILKNSFFSSDQTGIPSLDHLINLYVERNYLIWRIPDLQKLLKETAQLVIEVLETD 532

Query: 528 RNDAKDWACVRKEAFSSEKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH 587
           RNDAKDWACVRKEAFS +KNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH
Sbjct: 533 RNDAKDWACVRKEAFSCKKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH 592

Query: 588 VNPPDQRQPTREIANRSALAVLFESMLPWVNYGDERDVGVDEGNQFDEHDH 638
            NPP+QR PTREIANRSALAVLFESMLPWVNYGDERD+GVDEGNQFDEHDH
Sbjct: 593 ANPPNQRHPTREIANRSALAVLFESMLPWVNYGDERDIGVDEGNQFDEHDH 640

BLAST of Spg031386 vs. NCBI nr
Match: XP_022974270.1 (transcription factor 25 [Cucurbita maxima])

HSP 1 Score: 1052.4 bits (2720), Expect = 1.6e-303
Identity = 531/591 (89.85%), Postives = 558/591 (94.42%), Query Frame = 0

Query: 48  DEDDLGTHLEDKDNDPEAADEISVDMHKQFVRNGAKSAISTSNKKLKKKKKKKERENSSS 107
           DEDD   +   +D++ EAA+E+SV+MHK+FVRNG KSA STSNKKLKKKKKKKERENSSS
Sbjct: 53  DEDDSQIN-PQQDDEQEAAEEVSVNMHKEFVRNGTKSASSTSNKKLKKKKKKKERENSSS 112

Query: 108 TTVTDKILDKPLDLILESLSLDVKPSASSRQQGPEKAKSGA-EERNCGKQCLPSLLEVDP 167
           T  T+KILDKPLD+ILESLSLDV P A SRQQ PEKAK    EERN GKQCL SLLEVDP
Sbjct: 113 T--TNKILDKPLDVILESLSLDVNPFALSRQQDPEKAKDVVEEERNRGKQCLTSLLEVDP 172

Query: 168 KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHLARRTYLVSPSDHWPRWDG 227
           KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQ+RGGRRVNHL+R+TYLVSPSDHWPRWDG
Sbjct: 173 KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQIRGGRRVNHLSRKTYLVSPSDHWPRWDG 232

Query: 228 SISMEFLETRNGYHYFRYVYPPSYVEAQKAFEDAKSIHDLNGIASILLYHPYHLDSLITM 287
           S+SMEFLET+NGYHYFRYV+PPSYVEAQKAFE AKSIHDLNGIASILL+HPYHLDSLITM
Sbjct: 233 SLSMEFLETKNGYHYFRYVHPPSYVEAQKAFEAAKSIHDLNGIASILLHHPYHLDSLITM 292

Query: 288 AEYFKFVGDHEMSSDAVAKSLYALECAWHPMFTVLQGNCQLKISHETNKPMFTSLFTHVK 347
           AEYFKF GDHEMSSDAVAKSLYALECAWHPMFT LQGNCQLKISHETNKPMFTSLFTHVK
Sbjct: 293 AEYFKFAGDHEMSSDAVAKSLYALECAWHPMFTALQGNCQLKISHETNKPMFTSLFTHVK 352

Query: 348 YLDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYKNDTSLW 407
            LDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEY+WLERFAESYKNDTSLW
Sbjct: 353 NLDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYSWLERFAESYKNDTSLW 412

Query: 408 LFPNFSFSLSICRFYLEREEASKDDNVDATRANSRDLLKQALMLHPLVLKRLVEKVPLKE 467
           LFPNFSFSLS+CR+YLEREE SK+DN+DATRANS DLLKQALMLHPLVLKRLVEKVPLKE
Sbjct: 413 LFPNFSFSLSVCRYYLEREEDSKNDNLDATRANSTDLLKQALMLHPLVLKRLVEKVPLKE 472

Query: 468 QFWVHILKHSFFLPDQTGIPSLDHLINLYVERNYIIWRIPDLQKLLRETAQSVIEVLETD 527
           QFWVHILK+SFF  DQTGIPSLDHLINLYVERNY+IWRIPDLQKLL+ETAQ VIEVLETD
Sbjct: 473 QFWVHILKNSFFSSDQTGIPSLDHLINLYVERNYLIWRIPDLQKLLKETAQLVIEVLETD 532

Query: 528 RNDAKDWACVRKEAFSSEKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH 587
           RNDAKDWACVRKEAFS EKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH
Sbjct: 533 RNDAKDWACVRKEAFSCEKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH 592

Query: 588 VNPPDQRQPTREIANRSALAVLFESMLPWVNYGDERDVGVDEGNQFDEHDH 638
            NPP+QR PTREIANRSALAVLFESMLPWVNYGDERD+GVDEGNQFDEHDH
Sbjct: 593 ANPPNQRHPTREIANRSALAVLFESMLPWVNYGDERDIGVDEGNQFDEHDH 640

BLAST of Spg031386 vs. NCBI nr
Match: XP_023538951.1 (transcription factor 25-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1052.0 bits (2719), Expect = 2.1e-303
Identity = 531/591 (89.85%), Postives = 556/591 (94.08%), Query Frame = 0

Query: 48  DEDDLGTHLEDKDNDPEAADEISVDMHKQFVRNGAKSAISTSNKKLKKKKKKKERENSSS 107
           DEDD   +   +D+D E A+E+SV+MHK+FVRNG KSA STSNKKLKKKKKK ERENSSS
Sbjct: 53  DEDDSQIN-PQQDDDQEVAEEVSVNMHKEFVRNGTKSASSTSNKKLKKKKKKTERENSSS 112

Query: 108 TTVTDKILDKPLDLILESLSLDVKPSASSRQQGPEKAKSGA-EERNCGKQCLPSLLEVDP 167
           T  T+KILDKPLD+ILESLSLDV PSA SRQQ PEKAK    EERN GKQCL SLLEVDP
Sbjct: 113 T--TNKILDKPLDVILESLSLDVNPSALSRQQDPEKAKDVVEEERNRGKQCLTSLLEVDP 172

Query: 168 KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHLARRTYLVSPSDHWPRWDG 227
           KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHL+R+TYLVSPSDHWPRWDG
Sbjct: 173 KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHLSRKTYLVSPSDHWPRWDG 232

Query: 228 SISMEFLETRNGYHYFRYVYPPSYVEAQKAFEDAKSIHDLNGIASILLYHPYHLDSLITM 287
           S+SMEFLET+NGYHYFRYV+PPSYVEAQKAFE AKSIHDLNGIASILL+HPYHLDSLITM
Sbjct: 233 SLSMEFLETKNGYHYFRYVHPPSYVEAQKAFEAAKSIHDLNGIASILLHHPYHLDSLITM 292

Query: 288 AEYFKFVGDHEMSSDAVAKSLYALECAWHPMFTVLQGNCQLKISHETNKPMFTSLFTHVK 347
           AEYFKF GDHEMSSDAVAKSLYALECAWHPMFT LQGNCQLKISHETNKPMFTSLFTHVK
Sbjct: 293 AEYFKFAGDHEMSSDAVAKSLYALECAWHPMFTALQGNCQLKISHETNKPMFTSLFTHVK 352

Query: 348 YLDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYKNDTSLW 407
            LDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYKNDTSLW
Sbjct: 353 NLDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYKNDTSLW 412

Query: 408 LFPNFSFSLSICRFYLEREEASKDDNVDATRANSRDLLKQALMLHPLVLKRLVEKVPLKE 467
           LFPNFSFSLS+CR+YLEREE SK+DN+DATRANS DLLKQALMLHP VLKRLVEKVPLKE
Sbjct: 413 LFPNFSFSLSVCRYYLEREEDSKNDNLDATRANSTDLLKQALMLHPFVLKRLVEKVPLKE 472

Query: 468 QFWVHILKHSFFLPDQTGIPSLDHLINLYVERNYIIWRIPDLQKLLRETAQSVIEVLETD 527
           QFWVHILK+SFF  DQTGIPSLDHLINLYVERNY+IWRIPDLQKLL+ETAQ VIEVLETD
Sbjct: 473 QFWVHILKNSFFSSDQTGIPSLDHLINLYVERNYLIWRIPDLQKLLKETAQLVIEVLETD 532

Query: 528 RNDAKDWACVRKEAFSSEKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH 587
           RNDAKDWACVRKEAFS +KNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH
Sbjct: 533 RNDAKDWACVRKEAFSCDKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH 592

Query: 588 VNPPDQRQPTREIANRSALAVLFESMLPWVNYGDERDVGVDEGNQFDEHDH 638
            NPP+QR PTREIANRSALAVLFESMLPWVNYGDERD+GVDEGNQFDEHDH
Sbjct: 593 ANPPNQRHPTREIANRSALAVLFESMLPWVNYGDERDIGVDEGNQFDEHDH 640

BLAST of Spg031386 vs. ExPASy Swiss-Prot
Match: Q9BQ70 (Transcription factor 25 OS=Homo sapiens OX=9606 GN=TCF25 PE=1 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.3e-61
Identity = 162/553 (29.29%), Postives = 287/553 (51.90%), Query Frame = 0

Query: 87  STSNKKLKKKKKKKERENSSSTTVTDKILDKPLDLILESLSLDVKPSASSRQQGPEKAKS 146
           S ++ KL+KKKKK++ + SS+   ++  L+  +D ILE     ++ S    + GP    S
Sbjct: 119 SHASGKLRKKKKKQKNKKSSTGEASENGLE-DIDRILER----IEDSTGLNRPGPAPLSS 178

Query: 147 GAEERNCGKQCLPSLLEVDPKYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVN 206
                         +L V+ ++LN   EL+R FG++ +   ++  Q           R  
Sbjct: 179 RKH-----------VLYVEHRHLNPDTELKRYFGARAILGEQRPRQ-----------RQR 238

Query: 207 HLARRTYLVSPSDHWPRWD-GSISMEFLETRNGYHYFRYVYPPSYVEAQKAFEDAKSIHD 266
              + T+L +P   WPR+    +SM  LE++ G  +F + +   Y +AQ  F  A    +
Sbjct: 239 VYPKCTWLTTPKSTWPRYSKPGLSMRLLESKKGLSFFAFEHSEEYQQAQHKFLVAVESME 298

Query: 267 LNGIASILLYHPYHLDSLITMAEYFKFVGDHEMSSDAVAKSLYALECAWHPMFTVLQGNC 326
            N I  +L   PYH+DSL+ +++  +F  D EM+ D V ++LY++ECA+HP+F++  G C
Sbjct: 299 PNNIVVLLQTSPYHVDSLLQLSDACRFQEDQEMARDLVERALYSMECAFHPLFSLTSGAC 358

Query: 327 QLKISHETNKPMFTSLFTHVKYLDRRGCHRSALEVCKLLLSLDSD-DPMGALFFIDYLSL 386
           +L      N+  + +L+  + +L++RGC R+ALE CKL+LSL+ D DP+  L  ID+L+L
Sbjct: 359 RLDYRRPENRSFYLALYKQMSFLEKRGCPRTALEYCKLILSLEPDEDPLCMLLLIDHLAL 418

Query: 387 RAEEYTWLERFAESYKNDTSLWLFPNFSFSLSICRFYLEREEASKDDNVDATRANSRDLL 446
           RA  Y +L R  + ++   +L   PNF+FS+ +  F L ++    +    + R  +  L+
Sbjct: 419 RARNYEYLIRLFQEWEAHRNLSQLPNFAFSVPLAYFLLSQQTDLPECEQSSARQKASLLI 478

Query: 447 KQALMLHPLVLKRLVEKVPLKEQFWVHILKHSFFLPDQ--TGIPSLDHLINLYVERNYII 506
           +QAL + P VL  L+E   ++      +  H FF P+   +  P+L  L+NLY+ R++ +
Sbjct: 479 QQALTMFPGVLLPLLESCSVRPD--ASVSSHRFFGPNAEISQPPALSQLVNLYLGRSHFL 538

Query: 507 WRIPDLQKLLRETAQSVIEVLETDRNDAKDWACVRKEAF---SSEKNEYAHLLVSDFSDS 566
           W+ P     L E    V++ +  D  D    AC  +       + +N + H+++S+  ++
Sbjct: 539 WKEPATMSWLEENVHEVLQAV--DAGDPAVEACENRRKVLYQRAPRNIHRHVILSEIKEA 598

Query: 567 VSSAPPEALQDFVV--DPRMRDVQNIIQHVNPPDQRQPTREIANRSALAVLFESMLP-WV 626
           V++ PP+     V+  DP +     I  +V P    +    I++ + +A+ F S+LP + 
Sbjct: 599 VAALPPDVTTQSVMGFDP-LPPSDTIYSYVRP----ERLSPISHGNTIALFFRSLLPNYT 635

Query: 627 NYGDERDVGVDEG 630
             G+  + GV  G
Sbjct: 659 MEGERPEEGVAGG 635

BLAST of Spg031386 vs. ExPASy Swiss-Prot
Match: Q8R3L2 (Transcription factor 25 OS=Mus musculus OX=10090 GN=Tcf25 PE=1 SV=2)

HSP 1 Score: 237.3 bits (604), Expect = 4.9e-61
Identity = 159/553 (28.75%), Postives = 288/553 (52.08%), Query Frame = 0

Query: 87  STSNKKLKKKKKKKERENSSSTTVTDKILDKPLDLILESLSLDVKPSASSRQQGPEKAKS 146
           S ++ KL+KKKKK++ + S +   ++  L+  +D ILE     ++ S+     GP    S
Sbjct: 119 SNASGKLRKKKKKQKNKKSCTGESSENGLE-DIDRILER----IEDSSGFSHPGPPPLSS 178

Query: 147 GAEERNCGKQCLPSLLEVDPKYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVN 206
                         +L V+ ++LN   EL+R FG++ V   ++  Q           R  
Sbjct: 179 RKH-----------VLYVEHRHLNPDTELKRYFGARAVLGEQRPRQ-----------RQR 238

Query: 207 HLARRTYLVSPSDHWPRWD-GSISMEFLETRNGYHYFRYVYPPSYVEAQKAFEDAKSIHD 266
              + T+L +P   WPR+    +SM  LE++ G  +F + +   Y +AQ  F  A    +
Sbjct: 239 VYPKCTWLTTPKSTWPRYSKPGLSMRLLESKKGLSFFAFDHNEEYQQAQHKFLVAVESME 298

Query: 267 LNGIASILLYHPYHLDSLITMAEYFKFVGDHEMSSDAVAKSLYALECAWHPMFTVLQGNC 326
            N I  +L   PYH+DSL+ +++  +F  D EM+ D + ++LY++ECA+HP+F++  G C
Sbjct: 299 PNNIVVLLQTSPYHVDSLLQLSDACRFQEDQEMARDLIERALYSMECAFHPLFSLTSGTC 358

Query: 327 QLKISHETNKPMFTSLFTHVKYLDRRGCHRSALEVCKLLLSLDSD-DPMGALFFIDYLSL 386
           +L      N+  + +L+  + +L++RGC R+ALE CKL+LSL+ D DP+  L  ID+L+L
Sbjct: 359 RLDYRRPENRSFYLTLYKQMSFLEKRGCPRTALEYCKLILSLEPDEDPLCMLLLIDHLAL 418

Query: 387 RAEEYTWLERFAESYKNDTSLWLFPNFSFSLSICRFYLEREEASKDDNVDATRANSRDLL 446
           RA  Y +L R  + ++   +L   PNF+FS+ +  F L ++    +  + + R  +  L+
Sbjct: 419 RARNYEYLIRLFQEWEAHRNLSQLPNFAFSVPLAYFLLSQQTDLPEHELSSARQQASLLI 478

Query: 447 KQALMLHPLVLKRLVEKVPLKEQFWVHILKHSFFLPDQ--TGIPSLDHLINLYVERNYII 506
           +QAL + P VL  L+E   ++      +  H FF PD   +  P+L  L++LY+ R++ +
Sbjct: 479 QQALTMFPGVLMPLLEYCSVRPD--ATVSNHRFFGPDAEISQPPALGQLVSLYLGRSHFL 538

Query: 507 WRIPDLQKLLRETAQSVIEVLETDRNDAKDWACVRKEAF---SSEKNEYAHLLVSDFSDS 566
           W+ P +   L E    V++ +  D  D    AC  +       + +N + H+++S+  ++
Sbjct: 539 WKEPAIMSWLEENVHEVLQAV--DAGDPAVEACENRRKVLYQRAPRNIHRHVILSEIKEA 598

Query: 567 VSSAPPEALQDFVV--DPRMRDVQNIIQHVNPPDQRQPTREIANRSALAVLFESMLP-WV 626
           V++ P +     V+  DP +  +  I  +V P    +    +++ + +A+ F S+LP + 
Sbjct: 599 VAALPSDVTTQSVMGFDP-LPPLDTIYSYVRP----ERLSPVSHGNTIALFFRSLLPNYT 635

Query: 627 NYGDERDVGVDEG 630
             G+  + GV  G
Sbjct: 659 TEGERLEEGVAGG 635

BLAST of Spg031386 vs. ExPASy Swiss-Prot
Match: Q8T2A4 (Transcription factor 25 homolog OS=Dictyostelium discoideum OX=44689 GN=tcf25 PE=3 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 9.5e-49
Identity = 154/601 (25.62%), Postives = 279/601 (46.42%), Query Frame = 0

Query: 29  VPSTTTKFTFFNPNMDYRGDEDDLGTHLEDKDNDPEAADEISVDMHKQFVRNGAKSAIST 88
           V S   KF+  N + D   D+ D   + + KD++ E ++E   +   +  +  AK  +S+
Sbjct: 33  VNSVKPKFSMLNLS-DEDDDKSDQDENEDKKDSESEESEEEEEEEEIEKEKPQAKEQLSS 92

Query: 89  SNKKLKKKK---KKKERENSSSTTVTDKILDKPLDLILESLSLDVKPSASSRQQGPEKAK 148
             +K K+++   KKK+ +         +  +K  +L L  +     P   S  +  +K  
Sbjct: 93  KQQKQKQQQQASKKKQAKKKQQQKKKQQQQEKD-ELELNEIEKSSPPIILSEIEIKDKE- 152

Query: 149 SGAEERNCGKQCLPSLLEVDPKYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRV 208
                          L +++   LN  NEL+++FG K           SS  + +  + V
Sbjct: 153 ------------FRELFKINTVNLNPDNELKKLFGCK-----------SSDIKGQKKKSV 212

Query: 209 NHLARRTYL-VSPSDHWPR-WDGSISMEFL------------------------------ 268
           +H  ++ Y+ V P   WP    G + ME                                
Sbjct: 213 HHFKKKNYIFVQPKPEWPDVMGGFMVMEICNDDKVPSSPTNATTTTTTTTTTTNTVSPKL 272

Query: 269 ----ETRNGYHYFRYVYPPSYVEAQKAFEDAKSIHDLNGIASILLYHPYHLDSLITMAEY 328
               ++ +G +YF+  +  +Y   Q+ F  A + HD   +  I+ YHPYH+DSL+ + + 
Sbjct: 273 KSQQQSLDGINYFKIKWGQTYSNIQEEFYLALTTHDPMSLVEIIRYHPYHIDSLLQLGQV 332

Query: 329 FKFVGDHEMSSDAVAKSLYALECAWHPMFTVLQGNCQLKISHETNKPMFTSLFTHVKYLD 388
                D+  + D V  +++A E  +H +F  L GNC+ +  H+ NK  F ++F  ++ + 
Sbjct: 333 CLQTADYSKAGDFVEMAVFAFENVFHHLFNPLNGNCRFEYRHDQNKSCFLAIFRFIQIIG 392

Query: 389 RRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYK-----NDTS 448
           RR CHR+ALE+CK+LL+ D  DP+     IDY S+R+++Y +L       K     N   
Sbjct: 393 RRACHRTALELCKILLTWDYSDPLYVRLIIDYYSIRSKQYRFLVDLFTKLKTITPTNQGD 452

Query: 449 LWLFPNFSFSLSICRFYLEREEASKDDNVDATRANSRD---------LLKQALMLHPLVL 508
           L L PNF +S ++  ++LERE+++   +   + ++S+          LL++AL+  P+VL
Sbjct: 453 LSLLPNFCYSAALAMYHLEREKSTTTTSTSTSTSSSKSSSVEESSDILLQKALISFPMVL 512

Query: 509 KRLVEKVPLKEQFWVH------ILKHSFFLPDQTGIPSLDHLINLYVERNYIIWRIPDLQ 568
           + L+EK  LK  F +        L++  F  D      +DHL+ L+VERNY  W+ P++ 
Sbjct: 513 QPLLEK--LKTNFSIPKNGKIITLENDPFFKDDPLEKRIDHLVTLFVERNYQCWQTPEIL 572

Query: 569 KLLRETAQSVIEVLETDRNDAKDWACVRKEAFSSEKNE-YAHLLVSDFSDSVSSAPPEAL 570
             L+     V++ +       K  +   K  + +   E + HL++S++SD ++   P+ +
Sbjct: 573 DWLKSNVIIVLDKVAAKHPMVKQLSDSIKAEYETVNQEVFDHLVLSEYSDVINRLTPDII 605

BLAST of Spg031386 vs. ExPASy Swiss-Prot
Match: O13796 (Ribosome quality control complex subunit 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rqc1 PE=1 SV=2)

HSP 1 Score: 138.3 bits (347), Expect = 3.1e-31
Identity = 123/473 (26.00%), Postives = 211/473 (44.61%), Query Frame = 0

Query: 56  LEDKDN--DPEAADEISVDMHKQFVRNGAKSAISTSNKKLKKKKKKKERENSSSTTVTD- 115
           LE ++N  + EA   +S +   + +  G    +ST+ K   KKKKKK+++    T   D 
Sbjct: 47  LEKENNAINSEAEKSVSEEEQDEPLVEGESPIVSTNKKAKNKKKKKKQQKKKKVTGKRDL 106

Query: 116 ----------------KILDKPLDLILESLSLDVKPSASSRQQGPEKAKSGAEERNC--- 175
                           K +D  +D I E  + ++K     R+Q   +  +G EE      
Sbjct: 107 DNQSSDNEKLEGLESSKNIDDDIDEI-EKAAAELK--LKYREQDQVEHVAGVEESATIPL 166

Query: 176 ---GKQCLPSLLEVDPKYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHLAR 235
                + L  LL V+   LN   E+R+IFG  V    EK +  +    LR  R V     
Sbjct: 167 DKELDEKLNKLLGVNISMLNPDLEIRKIFGRIV----EKRSVNARHDNLRRKRHV----- 226

Query: 236 RTYLVSPSDHWPRWDGS-ISMEFL-ETRNGYHYFRYVYPPSYVEAQKAFEDAKSIHDLNG 295
              LV P + WP    S + M+   ++++   +F      +Y E Q+ FE     +D N 
Sbjct: 227 ---LVQPQEGWPPLVRSGLGMKLTGQSQDLECFFEITQSRAYQEVQETFEYYVQTYDPNN 286

Query: 296 IASILLYHPYHLDSLITMAEYFKFVGDHEMSSDAVAKSLYALECAWHPMFTVLQGNCQLK 355
           +  +L  HP+H+D+L+ ++E     GDHE+S++ VA+ LYA +   HP F +  G  +L 
Sbjct: 287 LLMLLRSHPFHIDTLLQVSEIIDQQGDHELSAELVARGLYAFDSILHPRFNLATGATRLP 346

Query: 356 ISHETNKPMFTSLFTHVKYLDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEE 415
            +  +N+ +F  ++ +++ L  RGC R+  E CK LL  D  DP      ID  +LR  E
Sbjct: 347 FAIPSNRRLFLCIWRYLQSLQSRGCWRTVFEFCKALLQFDMSDPYAIGTCIDIYALRRRE 406

Query: 416 YTWLERFAESYKNDTSLWLFPNFSFSLSICRFYLEREEASKDDNVDATRANSRDLLKQAL 475
           + W+  FA   +N   +   PN  +S ++  FY+  +      ++ A    +  +L + L
Sbjct: 407 FAWIIDFANYLENSNKISDTPNMLYSSALAMFYVHGDTTDTRASMLAAFERAPYMLSELL 466

Query: 476 MLHPLVLKRLVEKVPLKEQFWVHILKHSFFLPDQTGIPSLDHLINLYVERNYI 502
               +   +     P      +H   ++ +  D    P++   IN  +E+  +
Sbjct: 467 DTLNISFTKSSIPSPQDPVQELHSAMYALYAKDSWSDPTVLAFINSILEKETV 504

BLAST of Spg031386 vs. ExPASy TrEMBL
Match: A0A6J1F3V8 (transcription factor 25 OS=Cucurbita moschata OX=3662 GN=LOC111442146 PE=3 SV=1)

HSP 1 Score: 1058.5 bits (2736), Expect = 1.1e-305
Identity = 535/591 (90.52%), Postives = 559/591 (94.59%), Query Frame = 0

Query: 48  DEDDLGTHLEDKDNDPEAADEISVDMHKQFVRNGAKSAISTSNKKLKKKKKKKERENSSS 107
           DEDD   +   +D+D EAA+E+SV+MHK+FVRNG KSA STSNKKLKKKKKKKERENSSS
Sbjct: 53  DEDDSQIN-PQQDDDQEAAEEVSVNMHKEFVRNGTKSASSTSNKKLKKKKKKKERENSSS 112

Query: 108 TTVTDKILDKPLDLILESLSLDVKPSASSRQQGPEKAKSGA-EERNCGKQCLPSLLEVDP 167
           T  T+KILDKPLD+ILESLSLDV PSA SRQQ PEKAK    EERN GKQCL SLLEVDP
Sbjct: 113 T--TNKILDKPLDVILESLSLDVNPSALSRQQDPEKAKDVVEEERNRGKQCLTSLLEVDP 172

Query: 168 KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHLARRTYLVSPSDHWPRWDG 227
           KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHL+R+TYLVSPSDHWPRWDG
Sbjct: 173 KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHLSRKTYLVSPSDHWPRWDG 232

Query: 228 SISMEFLETRNGYHYFRYVYPPSYVEAQKAFEDAKSIHDLNGIASILLYHPYHLDSLITM 287
           S+SMEFLET+NGYHYFRYV+PPSYVEAQKAFE AKSIHDLNGIASILL+HPYHLDSLITM
Sbjct: 233 SLSMEFLETKNGYHYFRYVHPPSYVEAQKAFEAAKSIHDLNGIASILLHHPYHLDSLITM 292

Query: 288 AEYFKFVGDHEMSSDAVAKSLYALECAWHPMFTVLQGNCQLKISHETNKPMFTSLFTHVK 347
           AEYFKF GDHEMSSDAVAKSLYALECAWHPMFT LQGNCQLKISHETNKPMFTSLFTHVK
Sbjct: 293 AEYFKFAGDHEMSSDAVAKSLYALECAWHPMFTALQGNCQLKISHETNKPMFTSLFTHVK 352

Query: 348 YLDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYKNDTSLW 407
            LDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYKNDTSLW
Sbjct: 353 NLDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYKNDTSLW 412

Query: 408 LFPNFSFSLSICRFYLEREEASKDDNVDATRANSRDLLKQALMLHPLVLKRLVEKVPLKE 467
           LFPNFSFSLS+CR+YLEREE SK+DN+DATRANS DLLKQALMLHPLVLKRLVEKVPLKE
Sbjct: 413 LFPNFSFSLSVCRYYLEREEDSKNDNLDATRANSTDLLKQALMLHPLVLKRLVEKVPLKE 472

Query: 468 QFWVHILKHSFFLPDQTGIPSLDHLINLYVERNYIIWRIPDLQKLLRETAQSVIEVLETD 527
           QFWVHILK+SFF  DQTGIPSLDHLINLYVERNY+IWRIPDLQKLL+ETAQ VIEVLETD
Sbjct: 473 QFWVHILKNSFFSSDQTGIPSLDHLINLYVERNYLIWRIPDLQKLLKETAQLVIEVLETD 532

Query: 528 RNDAKDWACVRKEAFSSEKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH 587
           RNDAKDWACVRKEAFS EKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH
Sbjct: 533 RNDAKDWACVRKEAFSCEKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH 592

Query: 588 VNPPDQRQPTREIANRSALAVLFESMLPWVNYGDERDVGVDEGNQFDEHDH 638
            NPP+QR PTREIANRSALAVLFESMLPWVNYGDERD+GVDEGNQFDEHDH
Sbjct: 593 ANPPNQRHPTREIANRSALAVLFESMLPWVNYGDERDIGVDEGNQFDEHDH 640

BLAST of Spg031386 vs. ExPASy TrEMBL
Match: A0A6J1IDI7 (transcription factor 25 OS=Cucurbita maxima OX=3661 GN=LOC111472909 PE=3 SV=1)

HSP 1 Score: 1052.4 bits (2720), Expect = 7.8e-304
Identity = 531/591 (89.85%), Postives = 558/591 (94.42%), Query Frame = 0

Query: 48  DEDDLGTHLEDKDNDPEAADEISVDMHKQFVRNGAKSAISTSNKKLKKKKKKKERENSSS 107
           DEDD   +   +D++ EAA+E+SV+MHK+FVRNG KSA STSNKKLKKKKKKKERENSSS
Sbjct: 53  DEDDSQIN-PQQDDEQEAAEEVSVNMHKEFVRNGTKSASSTSNKKLKKKKKKKERENSSS 112

Query: 108 TTVTDKILDKPLDLILESLSLDVKPSASSRQQGPEKAKSGA-EERNCGKQCLPSLLEVDP 167
           T  T+KILDKPLD+ILESLSLDV P A SRQQ PEKAK    EERN GKQCL SLLEVDP
Sbjct: 113 T--TNKILDKPLDVILESLSLDVNPFALSRQQDPEKAKDVVEEERNRGKQCLTSLLEVDP 172

Query: 168 KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHLARRTYLVSPSDHWPRWDG 227
           KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQ+RGGRRVNHL+R+TYLVSPSDHWPRWDG
Sbjct: 173 KYLNAGNELRRIFGSKVVKSFEKNNQASSSRQIRGGRRVNHLSRKTYLVSPSDHWPRWDG 232

Query: 228 SISMEFLETRNGYHYFRYVYPPSYVEAQKAFEDAKSIHDLNGIASILLYHPYHLDSLITM 287
           S+SMEFLET+NGYHYFRYV+PPSYVEAQKAFE AKSIHDLNGIASILL+HPYHLDSLITM
Sbjct: 233 SLSMEFLETKNGYHYFRYVHPPSYVEAQKAFEAAKSIHDLNGIASILLHHPYHLDSLITM 292

Query: 288 AEYFKFVGDHEMSSDAVAKSLYALECAWHPMFTVLQGNCQLKISHETNKPMFTSLFTHVK 347
           AEYFKF GDHEMSSDAVAKSLYALECAWHPMFT LQGNCQLKISHETNKPMFTSLFTHVK
Sbjct: 293 AEYFKFAGDHEMSSDAVAKSLYALECAWHPMFTALQGNCQLKISHETNKPMFTSLFTHVK 352

Query: 348 YLDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYKNDTSLW 407
            LDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEY+WLERFAESYKNDTSLW
Sbjct: 353 NLDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYSWLERFAESYKNDTSLW 412

Query: 408 LFPNFSFSLSICRFYLEREEASKDDNVDATRANSRDLLKQALMLHPLVLKRLVEKVPLKE 467
           LFPNFSFSLS+CR+YLEREE SK+DN+DATRANS DLLKQALMLHPLVLKRLVEKVPLKE
Sbjct: 413 LFPNFSFSLSVCRYYLEREEDSKNDNLDATRANSTDLLKQALMLHPLVLKRLVEKVPLKE 472

Query: 468 QFWVHILKHSFFLPDQTGIPSLDHLINLYVERNYIIWRIPDLQKLLRETAQSVIEVLETD 527
           QFWVHILK+SFF  DQTGIPSLDHLINLYVERNY+IWRIPDLQKLL+ETAQ VIEVLETD
Sbjct: 473 QFWVHILKNSFFSSDQTGIPSLDHLINLYVERNYLIWRIPDLQKLLKETAQLVIEVLETD 532

Query: 528 RNDAKDWACVRKEAFSSEKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH 587
           RNDAKDWACVRKEAFS EKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH
Sbjct: 533 RNDAKDWACVRKEAFSCEKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQH 592

Query: 588 VNPPDQRQPTREIANRSALAVLFESMLPWVNYGDERDVGVDEGNQFDEHDH 638
            NPP+QR PTREIANRSALAVLFESMLPWVNYGDERD+GVDEGNQFDEHDH
Sbjct: 593 ANPPNQRHPTREIANRSALAVLFESMLPWVNYGDERDIGVDEGNQFDEHDH 640

BLAST of Spg031386 vs. ExPASy TrEMBL
Match: A0A6J1CYN2 (transcription factor 25 OS=Momordica charantia OX=3673 GN=LOC111015989 PE=3 SV=1)

HSP 1 Score: 1045.0 bits (2701), Expect = 1.2e-301
Identity = 525/588 (89.29%), Postives = 549/588 (93.37%), Query Frame = 0

Query: 50  DDLGTHLEDKDNDPEAADEISVDMHKQFVRNGAKSAISTSNKKLKKKKKKKERENSSSTT 109
           DD       +DND EA DEIS D+HKQFV NG +SAISTSNKKLKKKKKKKERE SSST 
Sbjct: 58  DDQEDDPPKQDNDQEAVDEISADLHKQFVHNGTRSAISTSNKKLKKKKKKKEREKSSST- 117

Query: 110 VTDKILDKPLDLILESLSLDVKPSASSRQQGPEKAKSGAEERNCGKQCLPSLLEVDPKYL 169
             DKILDKPLD+ILESLSLDV  SA S Q GPEKAK+G +ERN GKQCLPSLLEVDPKYL
Sbjct: 118 -XDKILDKPLDMILESLSLDVNTSALSLQHGPEKAKNGVDERNRGKQCLPSLLEVDPKYL 177

Query: 170 NAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHLARRTYLVSPSDHWPRWDGSIS 229
           NAGNELRRIFGSKVVKSFE+N+QASSSRQ+RGGRRVNHLAR+TYLVSPSDHWPR DGS+S
Sbjct: 178 NAGNELRRIFGSKVVKSFERNHQASSSRQVRGGRRVNHLARKTYLVSPSDHWPRLDGSLS 237

Query: 230 MEFLETRNGYHYFRYVYPPSYVEAQKAFEDAKSIHDLNGIASILLYHPYHLDSLITMAEY 289
           MEFL+TR+GYHYFRYV+ PSYVEAQ+ FE AKSIHDLNGIASILLYHPYHLDSLITM EY
Sbjct: 238 MEFLDTRDGYHYFRYVHLPSYVEAQRGFEAAKSIHDLNGIASILLYHPYHLDSLITMTEY 297

Query: 290 FKFVGDHEMSSDAVAKSLYALECAWHPMFTVLQGNCQLKISHETNKPMFTSLFTHVKYLD 349
           FKFVGDHEMS+DAVAKSLYALECAWHPMFTVLQGNCQLKISHETNKPMFTSLFTHVK LD
Sbjct: 298 FKFVGDHEMSADAVAKSLYALECAWHPMFTVLQGNCQLKISHETNKPMFTSLFTHVKNLD 357

Query: 350 RRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYKNDTSLWLFP 409
           RRGCHRSALEVCKLLLSLDSDDPMG+LFFIDYLSLRAEEYTWLE+FAESYKNDTSLWLFP
Sbjct: 358 RRGCHRSALEVCKLLLSLDSDDPMGSLFFIDYLSLRAEEYTWLEKFAESYKNDTSLWLFP 417

Query: 410 NFSFSLSICRFYLEREEASKDDNVDATRANSRDLLKQALMLHPLVLKRLVEKVPLKEQFW 469
           NFSFSLSI RFYLEREE  K DN+D TRANS DLLKQALMLHPLVLKRLVEKVPLKEQFW
Sbjct: 418 NFSFSLSISRFYLEREEPLKTDNLDCTRANSTDLLKQALMLHPLVLKRLVEKVPLKEQFW 477

Query: 470 VHILKHSFFLPDQTGIPSLDHLINLYVERNYIIWRIPDLQKLLRETAQSVIEVLETDRND 529
           VHILKHSFFLPDQTGIPSLDHLINLYVERNYIIWRIPDLQKLLRETAQ +IE LETDRND
Sbjct: 478 VHILKHSFFLPDQTGIPSLDHLINLYVERNYIIWRIPDLQKLLRETAQLIIETLETDRND 537

Query: 530 AKDWACVRKEAFSSEKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQHVNP 589
           AKDWACVR+EAF+SEKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPR+RDVQNIIQH NP
Sbjct: 538 AKDWACVRREAFTSEKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRVRDVQNIIQHANP 597

Query: 590 PDQRQPTREIANRSALAVLFESMLPWVNYGDERDVGVDEGNQFDEHDH 638
           PDQRQPTREIANRSALAVLFESMLPWVNYGDERDVGVD+GNQ DEHDH
Sbjct: 598 PDQRQPTREIANRSALAVLFESMLPWVNYGDERDVGVDDGNQPDEHDH 643

BLAST of Spg031386 vs. ExPASy TrEMBL
Match: A0A1S3AUW3 (LOW QUALITY PROTEIN: transcription factor 25 OS=Cucumis melo OX=3656 GN=LOC103483095 PE=3 SV=1)

HSP 1 Score: 1024.6 bits (2648), Expect = 1.7e-295
Identity = 517/578 (89.45%), Postives = 540/578 (93.43%), Query Frame = 0

Query: 59  KDNDPEAADEISVDMHKQFVRNGAKSAISTSNKKLKKKKKKKERENSSSTTVTDKILDKP 118
           +DND EA +EISVD  KQFVRNGAKSAISTSNKKLKKKKKKKERE+ SST  TD+ILDKP
Sbjct: 69  QDNDREAVEEISVDSRKQFVRNGAKSAISTSNKKLKKKKKKKERESFSST--TDRILDKP 128

Query: 119 LDLILESLSLDVKPSASSRQQGPEKAKSGAEERNCGKQCLPSLLEVDPKYLNAGNELRRI 178
           LD++L+SLSLD+ PSA S  QGPEKAK+GAEE   GKQC+PSLLEV+PKYLNAGNELRRI
Sbjct: 129 LDVLLKSLSLDIDPSAISSPQGPEKAKNGAEE--SGKQCMPSLLEVEPKYLNAGNELRRI 188

Query: 179 FGSKVVKSFEKNNQASSSRQLRGGRRVNHLARRTYLVSPSDHWPRWDGSISMEFLETRNG 238
           FG+KVVKSFEKNNQASSSRQLRGGRRVNHL+R+TYLVSPSDHWPRWDGS+SME+LE+RNG
Sbjct: 189 FGAKVVKSFEKNNQASSSRQLRGGRRVNHLSRKTYLVSPSDHWPRWDGSLSMEYLESRNG 248

Query: 239 YHYFRYVYPPSYVEAQKAFEDAKSIHDLNGIASILLYHPYHLDSLITMAEYFKFVGDHEM 298
           YHYFRYV+ PSY EAQKAFE A+SIHDLNGIASILLYHPYHLDSLITMAEYFKF GDHEM
Sbjct: 249 YHYFRYVHLPSYXEAQKAFEAARSIHDLNGIASILLYHPYHLDSLITMAEYFKFAGDHEM 308

Query: 299 SSDAVAKSLYALECAWHPMFTVLQGNCQLKISHETNKPMFTSLFTHVKYLDRRGCHRSAL 358
           SSDAVAKSLYALECAWHPMFTV QGNCQLKI HETNKPMFTSLFTHVK LDRRGCHRSAL
Sbjct: 309 SSDAVAKSLYALECAWHPMFTVFQGNCQLKIRHETNKPMFTSLFTHVKNLDRRGCHRSAL 368

Query: 359 EVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYKNDTSLWLFPNFSFSLSIC 418
           EVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERF ESYKNDTSLWLFPNFSFSLSIC
Sbjct: 369 EVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFCESYKNDTSLWLFPNFSFSLSIC 428

Query: 419 RFYLEREEASKDDNVDATRANSRDLLKQALMLHPLVLKRLVEKVPLKEQFWVHILKHSFF 478
           RF+LEREE SK DNVDATRANS DLLKQALMLHPLVLKRLVEKVPLKEQFWVHIL+HSFF
Sbjct: 429 RFFLEREEPSKVDNVDATRANSTDLLKQALMLHPLVLKRLVEKVPLKEQFWVHILEHSFF 488

Query: 479 LPDQTGIPSLDHLINLYVERNYIIWRIPDLQKLLRETAQSVIEVLETDRNDAKDWACVRK 538
           LP QTGIPSLDHLINLYVERNYIIWRIP LQK LRETAQ VIE LETD+NDAKDW CVRK
Sbjct: 489 LPAQTGIPSLDHLINLYVERNYIIWRIPYLQKFLRETAQLVIEALETDQNDAKDWTCVRK 548

Query: 539 EAFSSEKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNIIQHVNPPDQRQPTRE 598
           EAFSSEKNEYAHLLVSDFSDS+SS PPEALQDFVVDPRMRDVQNIIQH NPPD RQPT E
Sbjct: 549 EAFSSEKNEYAHLLVSDFSDSLSSXPPEALQDFVVDPRMRDVQNIIQHANPPDHRQPTHE 608

Query: 599 IANRSALAVLFESMLPWVNYGDERDVGVDEGNQFDEHD 637
           I NRSALAVLFESMLPWVNYGD  DVGVDEGNQFDEHD
Sbjct: 609 IENRSALAVLFESMLPWVNYGD--DVGVDEGNQFDEHD 640

BLAST of Spg031386 vs. ExPASy TrEMBL
Match: A0A0A0L889 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G116680 PE=3 SV=1)

HSP 1 Score: 959.9 bits (2480), Expect = 5.3e-276
Identity = 499/607 (82.21%), Postives = 530/607 (87.31%), Query Frame = 0

Query: 30  PSTTTKFTFFNPNMDYRGDEDDLGTHLEDKDNDPEAADEISVDMHKQFVRNGAKSAISTS 89
           PS+ T  +  NP  D   D++D       +DND EA +E+SVD HKQFV NGAKSAISTS
Sbjct: 36  PSSPTA-SSINP-FDLLIDDEDDSQIDPHQDNDQEAVEEVSVDRHKQFVCNGAKSAISTS 95

Query: 90  NKKLKKKKKKKERENSSSTTVTDKILDKPLDLILESLSLDVKPSASSRQQGPEKAKSGAE 149
           NKKLKKKKKKKERE+SSST  TDKILDKPLD+IL+SLSLD+ PSA    QGP+KAK+G E
Sbjct: 96  NKKLKKKKKKKERESSSST--TDKILDKPLDVILKSLSLDINPSALRSPQGPDKAKNGTE 155

Query: 150 ERNCGKQCLPSLLEVDPKYLNAGNELRRIFGSKVVKSFEKNNQASSSRQLRGGRRVNHLA 209
           E    KQC+PSLLEV+PKYLNAGNELRRIFG+KVVKSFEKNNQASSSRQLRGGRRVNHL+
Sbjct: 156 E--SVKQCMPSLLEVEPKYLNAGNELRRIFGAKVVKSFEKNNQASSSRQLRGGRRVNHLS 215

Query: 210 RRTYLVSPSDHWPRWDGSISMEFLETRNGYHYFRYVYPPSYVEAQKAFEDAKSIHDLNGI 269
           R+TYLVSPSDHWPRWDGS+SME+LE+RNGYHYFRYV+ PSYVEAQKAFE A+SIHDLNGI
Sbjct: 216 RKTYLVSPSDHWPRWDGSLSMEYLESRNGYHYFRYVHLPSYVEAQKAFEAARSIHDLNGI 275

Query: 270 ASILLYHPYHLDSLITMAEYFKFVGDHEMSSDAVAKSLYALECAWHPMFTVLQGNCQLKI 329
           ASILLYHPYHLDSLITMAEYFKF GDHEMSSDAVAKSLYALECAWHPMFTV QGNCQLKI
Sbjct: 276 ASILLYHPYHLDSLITMAEYFKFAGDHEMSSDAVAKSLYALECAWHPMFTVFQGNCQLKI 335

Query: 330 SHETNKPMFTSLFTHVKYLDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEY 389
            HETNKPMFTSLFTHVK LDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEY
Sbjct: 336 RHETNKPMFTSLFTHVKNLDRRGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEY 395

Query: 390 TWLERFAESYKNDTSLWLFPNFSFSLSICRFYLEREEASKDDNVDATRANSRDLLKQALM 449
           TWLERF ESYKNDTSLWLFPNFSFSLSICRF+LE +E SK DNVDATRANS DLLKQAL+
Sbjct: 396 TWLERFCESYKNDTSLWLFPNFSFSLSICRFFLEGKEPSKVDNVDATRANSTDLLKQALL 455

Query: 450 LHPLVLKRLVEKVPLKEQFWVHILKHSFFLPDQTGIPSLDHLINLYVERNYIIWRIPDLQ 509
           LHP VLKRLVEKVPLKEQFWV IL+HSFFLP QTGIPSLDHLINLYVERNYIIWRIP LQ
Sbjct: 456 LHPFVLKRLVEKVPLKEQFWVQILEHSFFLPAQTGIPSLDHLINLYVERNYIIWRIPYLQ 515

Query: 510 KLLRETAQSVIEVLETDRNDAKDWACVRKEAFSSEKNEYAHLLVSDFSDSVSSAPPEALQ 569
           K LRETAQ +++VL                   S    YAHLLVSDFSDS+SSAPPEALQ
Sbjct: 516 KFLRETAQ-LVKVL-------------------SFNARYAHLLVSDFSDSLSSAPPEALQ 575

Query: 570 DFVVDPRMRDVQNIIQHVNPPDQRQPTREIANRSALAVLFESMLPWVNYGDERDVGVDEG 629
           DFVVDPRMRDVQNIIQH NPPD RQPT EI NRSALAVLFESMLPWVNYGDERDVGVDEG
Sbjct: 576 DFVVDPRMRDVQNIIQHANPPDHRQPTHEIENRSALAVLFESMLPWVNYGDERDVGVDEG 616

Query: 630 NQFDEHD 637
           NQFDEHD
Sbjct: 636 NQFDEHD 616

BLAST of Spg031386 vs. TAIR 10
Match: AT2G46900.1 (CONTAINS InterPro DOMAIN/s: Basic helix-loop-helix, Nulp1-type (InterPro:IPR006994); Has 2929 Blast hits to 2464 proteins in 333 species: Archae - 2; Bacteria - 151; Metazoa - 913; Fungi - 372; Plants - 141; Viruses - 47; Other Eukaryotes - 1303 (source: NCBI BLink). )

HSP 1 Score: 588.6 bits (1516), Expect = 6.1e-168
Identity = 310/595 (52.10%), Postives = 412/595 (69.24%), Query Frame = 0

Query: 56  LEDKDNDPEAADEISVDMHKQFVRNGAKSAIS----TSNKKLKKKKKKKERENSSSTTVT 115
           L D D DPE  D+ ++   K    +   S        S  K KKKKKKK +E+ S+    
Sbjct: 45  LNDGDEDPEEIDDETIAEKKNEDADHQSSVADHVQPVSKNKSKKKKKKKNKESGSNVPKA 104

Query: 116 DKILDKPLDLILESLSLDVKPSASSRQQGPEKAKSGAEERNCGKQCLPSLLEVDPKYLNA 175
           +  LD+     LE+L L    +A+S+Q   ++ K  A+     K     +LE+D KYLN 
Sbjct: 105 EMTLDE----TLEALGL----NANSKQDKVQETKPNADSSK--KASSRFVLEIDSKYLNL 164

Query: 176 GNELRRIFGSKVVKSFE-KNNQASSSRQLRGGRRVNHLARRTYLVSPSDHWPRWDGSISM 235
            NELRR +GSK ++SFE ++     S   RGGRR  H   +T LVSP ++W RWD S SM
Sbjct: 165 ENELRRTYGSKAMRSFESESGSQGGSSSRRGGRRGVHHITKTVLVSPKENWSRWDRSFSM 224

Query: 236 EFLETRNGYHYFRYVYPPSYVEAQKAFEDAKSIHDLNGIASILLYHPYHLDSLITMAEYF 295
           EFLET++G +YFRY +  SY +AQ+AF+ A++IHDLNG+AS+L+++PYH++SLITMA+YF
Sbjct: 225 EFLETKDGNNYFRYTHSSSYEQAQRAFQAAQAIHDLNGVASVLIHNPYHIESLITMADYF 284

Query: 296 KFVGDHEMSSDAVAKSLYALECAWHPMFTVLQGNCQLKISHETNKPMFTSLFTHVKYLDR 355
           KFVG+++M++D++ K LY LE AWHPMFT  QGNC+L+ +H+ NK  F +LFTH++ +DR
Sbjct: 285 KFVGENDMAADSIGKCLYGLERAWHPMFTPFQGNCRLEFNHDENKLFFKTLFTHMRNMDR 344

Query: 356 RGCHRSALEVCKLLLSLDSDDPMGALFFIDYLSLRAEEYTWLERFAESYKNDTSLWLFPN 415
           RGCHRSALEVCKLLLSLD+ +P+GALF +DY +LRAEEY WLE+F+E Y+ND SLWLFPN
Sbjct: 345 RGCHRSALEVCKLLLSLDTSNPVGALFCVDYFALRAEEYAWLEQFSEEYRNDNSLWLFPN 404

Query: 416 FSFSLSICRFYLEREEASKDDNV---DATRANSRDLLKQALMLHPLVLKRLVEKVPLKEQ 475
           FS+SL+I R YLE+ E +        D ++ +S DL+ QAL LHP VL +LVEKVPLK+Q
Sbjct: 405 FSYSLAIARVYLEKMEPTSSSEATPRDTSKQSSLDLMTQALKLHPTVLNKLVEKVPLKDQ 464

Query: 476 FWVHILKHSFFLPDQTGIPSLDHLINLYVERNYIIWRIPDLQKLLRETAQSVIEVLETDR 535
            W  ILKHS+F  D++ IPSLDHLI +YVERNY+IWR+PD+QKLLR  A  VIE LE D 
Sbjct: 465 AWTKILKHSYFRSDESKIPSLDHLIKIYVERNYLIWRLPDVQKLLRSAADLVIESLEQDG 524

Query: 536 NDAKDWACVRKEAFSSEKNEYAHLLVSDFSDSVSSAPPEALQDFVVDPRMRDVQNII--- 595
            +A+ W CVR EAFSSE N+Y+HL   DFSDS+ + PP+ LQ+FV DPRM   + +    
Sbjct: 525 TEAESWLCVRLEAFSSENNQYSHLSTHDFSDSMPTLPPDNLQNFVADPRMVGGEQMAEGG 584

Query: 596 ---QHVNPPDQRQPTREIANRSALAVLFESMLPWVNYGD-ERDVGVDEGNQFDEH 636
              QH  PP +    R++ANRS LAVL ES+LPW N+GD E ++  D  +Q D +
Sbjct: 585 GRGQHQAPPPR---PRDLANRSPLAVLIESILPWANFGDGEDEIAHDAHHQPDNN 626