Spg031349 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg031349
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	2'-phosphotransferase
Location	scaffold11: 42602747 .. 42607132 (-)
RNA-Seq Expression	Spg031349
Synteny	Spg031349

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTAAAACCACCGTCGTCTTCAACCTTTTTCCCTTCGCGATGTGGGCTTCTGTTGTTACCAACTGCGGCATCAAAATTCTGCGCTACCGTCCCCTTTTGCTTCCACGTGTCCTCATCGCTCCAATTCCCCTCCCCATCGCTCCTCCTGTATCTTCCATGGATAGAAGCAAAGCCTCCTCCTTTTCTTCTTCTGCCCATTCCAGTAAAAGGTTGATTATTCTCCTTCATCATCCTTCAATCGATGGAAAACTTCCTAGTTTTTCTGCTTCGTTTTTCATATTTTAGATTCTCGAATTCGTTGCTTGTGTTTTTTTTTATCACGATTTTGGATGTGTTTGTTGAAATTGTAGTCGTGGTTATTGATTCTGTTCTTTGTACTGATTTTTTCCGATCAGTGGTGGAAGAGGAAGAGGTTTGGACATGAGGAATGACAGAGAAAGAACGAGAGGCCGCGGTGGCGGAGGCGGCGGCTCTGGTAAAGACAAAATCGACGCCCTTGGTAGACTCTTGTAAGCATATTTCGTCTGTTGGTTTCTTGTTAAGGCGTTAATTTAATCATCTCATATTAGTTCTCAAACTTATTGCTTATTAATCCTGTGGAAGTTTCTGTAGTTGCTTTCTTAGTGAGAGTAGTGTTATTCTATCTATGGTTAAACTATGATTCATGAAGCTGAAGCTCGATTGACTTGAATTTTGATATTTTCTGATTTTTCTCTTCTACACTTGCTGGCTTGGCTATAATAATTTTCTTGGCCGAGGACAAAGGAAAGTGGAATAGGAGGATTATACTCCTACCTTAAATCACATGTACTTGATCGGTTAACTGCTGGCTATATTGATTTCAGCATTGCATTTCTGCTCGAGAAATTTTCAGAGGGAATAGAATGTAATCTGATGATCTATATGTTATATTTCCTTTCAGTTGTTGAAACTTGAATGAACATTTGGGAATGCTTGAGATTAAGCACTTGGGACCTTTCGAGTTTTGCGTTAAAATCTTAATAACTTAAACCTTTTGAGGTGGTTTGCTGACAATTTCAAGATGAGTTGTTGCATTTGTTCCTATTCTTTACTGTTATAACTATATTGGCTGGTGAAGAATGAATAAGCCCAAGGAGGAGTGTGAGAGGAAGGCTAGGTTATGAATATAAGATTTGTTTTTACAGCAATGTATATTTCACTGTTTATCCATCAATTGATATTTGCCCCTTTTAATGAACTTCAGGACACGCATCTTGCGGCATATGGCTTCCGAGTTAAACTTGAATATGAGAAGCGATGGATATGTCAAAGTTCAGGATTTGCTAAAGTTGAATTTGAAAACATTTGCTAATATCCCACTCAGATCGCACACAATTGATGACATAAGGGAGGTTGGTTGAATAATTTTCTTGCTTACTTGCTTTCGCATTAGTTTAATTTATAGAATCATTCTGGTATGTAACTTGGGTTTTCCATTGCTCAACAACTATTAATCTTGATCCCCTACCTCTAGAGGATTATTTATTTGCTAAGCTCAATTAAAACTTTCACCAAATAAAGGGCTTAGAGGGAATGAATTCAAGCCATGATGTCCATTTACCCAGGATTTAATATCCTATGAGTCTTCTTAGGATCCAAATGTAGTAGGGTCAGACAGTTGTCCGTGAGAATAGTCGAGGTGCATGCAAGCTGGTTTAGACATTCAAGGATATTAAGAAAAAGAAAATTTTAAACTTTCACCACACATCTACTCTATGCTGCACCTTTGATCCTTTTATCCTTGCTTACTTTCTCAGTTAGTTCCACTAATGTATTTCCTCTTCCCTGACAACTTGTAAATTTGCAGTTCAGTTTTGTTTTTGTAAGCATCTAGCATTGATGAATAGTTTTTTCTTTTTAATGGCAGGCTGTCAGGAAGGATAATAAGCAGCGTTTTGGCCTCCTGGAAGAGGATGGAGAGCTCTTAATTCGAGCGAACCAAGGCCATACAGTCACGGTTATTTTCTTTCTTTATAAACCATTGAATACATTACTGTTAGCCTTTTAGAAAATGTTGGGGGACTGTCAATCAATCTGATTGATTGGAAAATTGTTGATTGCTTCACGTTAGCTTATATGCATTTGATGGTTTTTGACATTAACTTATATGTGGATTAAAAAAATCATTTCTCCTTGTTTTCTATTGTTTCTCTGTTTTGTGACAATCCATCAGCCTTGTAAGATTGGTATATTGTTCGCTATACAGGATTGTTTATATGAGGCTTTTCCACTCCTTTTTATAACCTCTTCTAGATAAAACCATCTCTCTTAAGATTGTGATAATGGAACTAGATTGTCTACACCATCTTAAATGGAAAACACATTACTTGCAGGTGGTTGAAACTGAAAGCTTACTAAAACCTATTCTTTCGGCCGAGGAGGTTCCAGGTACTATGATTTGATGCCCTTAAACTTTTCTACTTTTCCCATATATCATCCTTTCCCCCTTGATGTGCACGATGTGAATATCGATGCATTTTAAAATTTCAACTTCTATTTTTGATATTTAATTAATCACTTTCCTTTTCTCCCTCTCTCCATGGCAGTATGTGTACATGGTACTTATAAGAAGAATTTGGAATCAATTCTGGAATCTGGTTTGAAACGCATGAAAAGATTGCATGTTCACTTCTCATGTGGTTTGCCGACAGATGGGGAGGTTATCAGTGGTAAGTTAAGAAAATCTTATTGTTACATTTCCCTGAAGAATTTATCCGATAAAACCACGATGCCACTTTGTTTGGCAATTAATTGCTTCTGCAATTGCACATCTTCACCTACTTATCTCTTCATAAGTGGTAAACCAGGAAGAACCGTACGGTTTAGAATCTACTTTTTGGTAAAAGTAAATGAAATGCCTTTATTCATGTATTCTGTTGAGGTTAAATTTTTCAAAGAGTAAAAGAAAAGGATCCACGATTAGACTTTCTCAACCATTTTTCATATATCTATGATTATACAAGATAAAGAAAAAGGACACTGACATGAATACAAGGCCATCGGTTTACTTTTCTTGCACTAGGAGGAAGAAGCGTGGAGCTGAGAAGGGCAAATAGAGTCTTGTGGGCCAAAGGAAAGTAGTTAATTGGGGTAGGGTCATCTTTTAGTCATCGTTTGTTAATATTATTGAGGTCAGCAAACCAATTTGAAGTTGGTTTGTTGGATGTTGAGAGCTGGCGGATTCTGAGATACATGTACTTAGAAGGGGAGAGGGAAAGAGTTGGGGGTAGTTGTAAGTTGAACTTGGAAATAGTTTAGCCTTTGGAGAGGGACACATTTATATCCTTCTCCTATCATCAGCAACAATTATTTTCTTTCCCGAGGCTTATCGGACAGACATGTTTAGAAGTGATTTTGGAATGCCTGAAATCATTTTTATCATGCCCTTTTTAGTGATTTTGGACCAGATAAAAGTGATTTTGGCCCTTGCAAAGTCACTCTCAAACACACCCTTAGTCTTATAATTAACCTAAATTAGTAAATTGGGGAGACCAAGCTAATTTTATTATTTTGTTTTAATCTGACAGGTATGAGACGTGATGTTAATGTTTTAATCTTTCTTGATGTCAAGAAAGCTCTGGATGGTATTGTTGAACCCTCCTCTCTCTCTCTTTTTTCTTATGGGCATTGTATGTTATAAAGGTGAAACGTGATGGTAACTGATGTTTTTGCTAAAACATTCTATTTCAGATGGAATGAAGCTTTACATTTCGGACAACAAGGTCATTCTGACTGAAGGTTTCGATGGCGTCGTGGTAGTAAAATACTTCCAAAAAATTGAGTCATGGCCGAATCGACAACCTATTCCTTTTTGAGCCTGGATCATATGGTACATGATCAATCATTTCTTGAAAATATTGAGTTATGTTTAGCTTTAGATCTAGACAAGATATTAACACAGTCAATATTTTAGTCTTGACCCAATTATAACTCGAAATTTCCACTTCGATCCAAACCAGATTGTCCTACGCTAACTTTGATGGCACGAGTTTCATACGAATTGGGAACTTGGGGAATCTATGAACTTCTTGGTGAATCATTCATGAAGTATTGGCAACAAGTACAGATTTCTTCGTGAAGTAATGCAAGCCATTTTTAATGTCACAAGTTCACAGATATTGCCTCATTGGAGACTGATATGTCAGCCAATCATGGATATTAGTATTACTAACAATTCTTATGTACACAAATACAACATATATATACATGGCTTGATTTGTTTGATATTGACTCATATTTTGATATGTTTTGGTGGTGGTTGGAAGTTTTAGTTTCTATTCTCTGTGACATATTAACTTTCTTTTTTGATGGTTGAAGTTGATGAGTGTATCTCATTAAATCAAGAAATTCTTAATAGATAAGCCG

mRNA sequence

ATGTGGGCTTCTGTTGTTACCAACTGCGGCATCAAAATTCTGCGCTACCGTCCCCTTTTGCTTCCACGTGTCCTCATCGCTCCAATTCCCCTCCCCATCGCTCCTCCTGTATCTTCCATGGATAGAAGCAAAGCCTCCTCCTTTTCTTCTTCTGCCCATTCCAGTAAAAGTGGTGGAAGAGGAAGAGGTTTGGACATGAGGAATGACAGAGAAAGAACGAGAGGCCGCGGTGGCGGAGGCGGCGGCTCTGGTAAAGACAAAATCGACGCCCTTGGTAGACTCTTGACACGCATCTTGCGGCATATGGCTTCCGAGTTAAACTTGAATATGAGAAGCGATGGATATGTCAAAGTTCAGGATTTGCTAAAGTTGAATTTGAAAACATTTGCTAATATCCCACTCAGATCGCACACAATTGATGACATAAGGGAGGCTGTCAGGAAGGATAATAAGCAGCGTTTTGGCCTCCTGGAAGAGGATGGAGAGCTCTTAATTCGAGCGAACCAAGGCCATACAGTCACGGTGGTTGAAACTGAAAGCTTACTAAAACCTATTCTTTCGGCCGAGGAGGTTCCAGTATGTGTACATGGTACTTATAAGAAGAATTTGGAATCAATTCTGGAATCTGGTTTGAAACGCATGAAAAGATTGCATGTTCACTTCTCATGTGGTTTGCCGACAGATGGGGAGGTTATCAGTGGTAAGTTAAGAAAATCTTATTGTTACATTTCCCTGAAGAATTTATCCGATAAAACCACGATGCCACTTTGTTTGGCAATTAATTGCTTCTGCAATTGCACATCTTCACCTACTTATCTCTTCATAAGTGGTAAACCAGGAAGAACCGTACGGTCAGCAAACCAATTTGAAGTTGGTTTGTTGGATGTTGAGAGCTGGCGGATTCTGAGATACATGTACTTAGAAGGGGAGAGGGAAAGAGTTGGGGGTAGTTGTATGAGACGTGATGTTAATGTTTTAATCTTTCTTGATGTCAAGAAAGCTCTGGATGATGGAATGAAGCTTTACATTTCGGACAACAAGGTCATTCTGACTGAAGGTTTCGATGGCGTCGTGGTAGTAAAATACTTCCAAAAAATTGAGTCATGGCCGAATCGACAACCTATTCCTTTTTGA

Coding sequence (CDS)

ATGTGGGCTTCTGTTGTTACCAACTGCGGCATCAAAATTCTGCGCTACCGTCCCCTTTTGCTTCCACGTGTCCTCATCGCTCCAATTCCCCTCCCCATCGCTCCTCCTGTATCTTCCATGGATAGAAGCAAAGCCTCCTCCTTTTCTTCTTCTGCCCATTCCAGTAAAAGTGGTGGAAGAGGAAGAGGTTTGGACATGAGGAATGACAGAGAAAGAACGAGAGGCCGCGGTGGCGGAGGCGGCGGCTCTGGTAAAGACAAAATCGACGCCCTTGGTAGACTCTTGACACGCATCTTGCGGCATATGGCTTCCGAGTTAAACTTGAATATGAGAAGCGATGGATATGTCAAAGTTCAGGATTTGCTAAAGTTGAATTTGAAAACATTTGCTAATATCCCACTCAGATCGCACACAATTGATGACATAAGGGAGGCTGTCAGGAAGGATAATAAGCAGCGTTTTGGCCTCCTGGAAGAGGATGGAGAGCTCTTAATTCGAGCGAACCAAGGCCATACAGTCACGGTGGTTGAAACTGAAAGCTTACTAAAACCTATTCTTTCGGCCGAGGAGGTTCCAGTATGTGTACATGGTACTTATAAGAAGAATTTGGAATCAATTCTGGAATCTGGTTTGAAACGCATGAAAAGATTGCATGTTCACTTCTCATGTGGTTTGCCGACAGATGGGGAGGTTATCAGTGGTAAGTTAAGAAAATCTTATTGTTACATTTCCCTGAAGAATTTATCCGATAAAACCACGATGCCACTTTGTTTGGCAATTAATTGCTTCTGCAATTGCACATCTTCACCTACTTATCTCTTCATAAGTGGTAAACCAGGAAGAACCGTACGGTCAGCAAACCAATTTGAAGTTGGTTTGTTGGATGTTGAGAGCTGGCGGATTCTGAGATACATGTACTTAGAAGGGGAGAGGGAAAGAGTTGGGGGTAGTTGTATGAGACGTGATGTTAATGTTTTAATCTTTCTTGATGTCAAGAAAGCTCTGGATGATGGAATGAAGCTTTACATTTCGGACAACAAGGTCATTCTGACTGAAGGTTTCGATGGCGTCGTGGTAGTAAAATACTTCCAAAAAATTGAGTCATGGCCGAATCGACAACCTATTCCTTTTTGA

Protein sequence

MWASVVTNCGIKILRYRPLLLPRVLIAPIPLPIAPPVSSMDRSKASSFSSSAHSSKSGGRGRGLDMRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEEDGELLIRANQGHTVTVVETESLLKPILSAEEVPVCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGKLRKSYCYISLKNLSDKTTMPLCLAINCFCNCTSSPTYLFISGKPGRTVRSANQFEVGLLDVESWRILRYMYLEGERERVGGSCMRRDVNVLIFLDVKKALDDGMKLYISDNKVILTEGFDGVVVVKYFQKIESWPNRQPIPF

Homology

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BLAST of Spg031349 vs. NCBI nr
Match: XP_022147085.1 (tRNA 2'-phosphotransferase 1 [Momordica charantia] >XP_022147086.1 tRNA 2'-phosphotransferase 1 [Momordica charantia])

HSP 1 Score: 495.4 bits (1274), Expect = 4.5e-136
Identity = 274/377 (72.68%), Postives = 280/377 (74.27%), Query Frame = 0


Query: 1   MWASVVTNCGIKILRYRPLLLPRVLIAPIPLPIAPPVSSMDRSKASSFSSSAHSSKSGGR 60
           MWA  VTNCGIKILRYRPLLLPRVLI PIPLPI P V SMD    SSF+S AHSSKSGGR
Sbjct: 1   MWA-CVTNCGIKILRYRPLLLPRVLITPIPLPIPPAVFSMDNPNTSSFASFAHSSKSGGR 60

Query: 61  GRGLDMRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQD 120
           GRGLDM NDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQD
Sbjct: 61  GRGLDMTNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQD 120

Query: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEEDGELLIRANQGHTVTVVETES 180
           LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEE+GELLIRANQGHTVTVVETES
Sbjct: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGELLIRANQGHTVTVVETES 180

Query: 181 LLKPILSAEEVPVCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGKLRKSY 240
           LL+PILSAEEVPVCVHG+YKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISG      
Sbjct: 181 LLRPILSAEEVPVCVHGSYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISG------ 240

Query: 241 CYISLKNLSDKTTMPLCLAINCFCNCTSSPTYLFISGKPGRTVRSANQFEVGLLDVESWR 300
                                                                       
Sbjct: 241 ------------------------------------------------------------ 292

Query: 301 ILRYMYLEGERERVGGSCMRRDVNVLIFLDVKKALDDGMKLYISDNKVILTEGFDGVVVV 360
                             MRRDVNVLIFLDVKKALDDGMKLY+SDNKVILTEGFDGVV V
Sbjct: 301 ------------------MRRDVNVLIFLDVKKALDDGMKLYVSDNKVILTEGFDGVVAV 292

Query: 361 KYFQKIESWPNRQPIPF 378
           KYFQKIESWP+RQPIPF
Sbjct: 361 KYFQKIESWPSRQPIPF 292

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BLAST of Spg031349 vs. NCBI nr
Match: XP_038893116.1 (tRNA 2'-phosphotransferase 1 [Benincasa hispida])

HSP 1 Score: 494.6 bits (1272), Expect = 7.7e-136
Identity = 271/377 (71.88%), Postives = 280/377 (74.27%), Query Frame = 0


Query: 1   MWASVVTNCGIKILRYRPLLLPRVLIAPIPLPIAPPVSSMDRSKASSFSSSAHSSKSGGR 60
           MWASVVTNCGIKILR RPLLLPRVLI PIPLP++P + +MDR   SS SS AHSS+SGGR
Sbjct: 1   MWASVVTNCGIKILRCRPLLLPRVLINPIPLPVSPSLFAMDRINTSSSSSFAHSSRSGGR 60

Query: 61  GRGLDMRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQD 120
           GRGLD+RNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMA+ELNLNMRSDGYVKVQD
Sbjct: 61  GRGLDLRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMAAELNLNMRSDGYVKVQD 120

Query: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEEDGELLIRANQGHTVTVVETES 180
           LLKLNLKTFAN PLRSHTIDDIREAVRKDNKQRF LLEE+GELLIRANQGHTV VVETES
Sbjct: 121 LLKLNLKTFANTPLRSHTIDDIREAVRKDNKQRFSLLEENGELLIRANQGHTVAVVETES 180

Query: 181 LLKPILSAEEVPVCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGKLRKSY 240
           LLKPILSAEEVPVCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISG      
Sbjct: 181 LLKPILSAEEVPVCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISG------ 240

Query: 241 CYISLKNLSDKTTMPLCLAINCFCNCTSSPTYLFISGKPGRTVRSANQFEVGLLDVESWR 300
                                                                       
Sbjct: 241 ------------------------------------------------------------ 293

Query: 301 ILRYMYLEGERERVGGSCMRRDVNVLIFLDVKKALDDGMKLYISDNKVILTEGFDGVVVV 360
                             MRRDVN+LIFLDVKKALDDGMKLYISDNKVILTEGFDGVV V
Sbjct: 301 ------------------MRRDVNILIFLDVKKALDDGMKLYISDNKVILTEGFDGVVAV 293

Query: 361 KYFQKIESWPNRQPIPF 378
           KYFQKIESWPNRQPIPF
Sbjct: 361 KYFQKIESWPNRQPIPF 293

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BLAST of Spg031349 vs. NCBI nr
Match: TYK17519.1 (tRNA 2'-phosphotransferase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 488.4 bits (1256), Expect = 5.5e-134
Identity = 267/377 (70.82%), Postives = 280/377 (74.27%), Query Frame = 0


Query: 1   MWASVVTNCGIKILRYRPLLLPRVLIAPIPLPIAPPVSSMDRSKASSFSSSAHSSKSGGR 60
           MWASVVTNCGIKILR R LLLPRVLI PIPLPI+P + SMDRS  +S SS AHSSKSGGR
Sbjct: 1   MWASVVTNCGIKILRCRSLLLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 61  GRGLDMRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQD 120
           GRGLD+RNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMR+DGYVKV+D
Sbjct: 61  GRGLDLRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRNDGYVKVED 120

Query: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEEDGELLIRANQGHTVTVVETES 180
           LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRF LL+E+GELLIRANQGHTVT VETES
Sbjct: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLDENGELLIRANQGHTVTAVETES 180

Query: 181 LLKPILSAEEVPVCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGKLRKSY 240
           LLKPILSAEEVPVCVHGTYKKNLESIL+SGLKRMKRLHVHFSCGLPTDGEVISG      
Sbjct: 181 LLKPILSAEEVPVCVHGTYKKNLESILDSGLKRMKRLHVHFSCGLPTDGEVISG------ 240

Query: 241 CYISLKNLSDKTTMPLCLAINCFCNCTSSPTYLFISGKPGRTVRSANQFEVGLLDVESWR 300
                                                                       
Sbjct: 241 ------------------------------------------------------------ 293

Query: 301 ILRYMYLEGERERVGGSCMRRDVNVLIFLDVKKALDDGMKLYISDNKVILTEGFDGVVVV 360
                             MRRDVNVLIFLDV+KAL+DGMKLYISDN+VILTEGFDG V +
Sbjct: 301 ------------------MRRDVNVLIFLDVQKALEDGMKLYISDNRVILTEGFDGTVPI 293

Query: 361 KYFQKIESWPNRQPIPF 378
           KYFQKIESWPNRQPIPF
Sbjct: 361 KYFQKIESWPNRQPIPF 293

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BLAST of Spg031349 vs. NCBI nr
Match: XP_004133917.2 (tRNA 2'-phosphotransferase 1 isoform X1 [Cucumis sativus] >KGN56605.1 hypothetical protein Csa_009546 [Cucumis sativus])

HSP 1 Score: 486.1 bits (1250), Expect = 2.7e-133
Identity = 265/377 (70.29%), Postives = 279/377 (74.01%), Query Frame = 0


Query: 1   MWASVVTNCGIKILRYRPLLLPRVLIAPIPLPIAPPVSSMDRSKASSFSSSAHSSKSGGR 60
           MWASVVTNCGIKILR RPL LPRVLI PIPLPI+P + SMDRS  +S SS AHSSKSGGR
Sbjct: 1   MWASVVTNCGIKILRCRPLWLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 61  GRGLDMRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQD 120
           GR LD+RNDRERTRGRGGGGGGSGKDKIDALGRLLTR+LRHMASELNLNMRSDGYVKV+D
Sbjct: 61  GRDLDLRNDRERTRGRGGGGGGSGKDKIDALGRLLTRVLRHMASELNLNMRSDGYVKVED 120

Query: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEEDGELLIRANQGHTVTVVETES 180
           LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRF  L+E+GELLIRANQGHTVT VETES
Sbjct: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSFLDENGELLIRANQGHTVTAVETES 180

Query: 181 LLKPILSAEEVPVCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGKLRKSY 240
           LLKPILSAEEVPVCVHGTYKKNLESIL+SGLKRMKRLHVHFSCGLPTDGEVISG      
Sbjct: 181 LLKPILSAEEVPVCVHGTYKKNLESILDSGLKRMKRLHVHFSCGLPTDGEVISG------ 240

Query: 241 CYISLKNLSDKTTMPLCLAINCFCNCTSSPTYLFISGKPGRTVRSANQFEVGLLDVESWR 300
                                                                       
Sbjct: 241 ------------------------------------------------------------ 293

Query: 301 ILRYMYLEGERERVGGSCMRRDVNVLIFLDVKKALDDGMKLYISDNKVILTEGFDGVVVV 360
                             MRRDVNVLIFLDV+KAL+DGMKLYISDN+VILTEGFDGVV +
Sbjct: 301 ------------------MRRDVNVLIFLDVQKALEDGMKLYISDNRVILTEGFDGVVPM 293

Query: 361 KYFQKIESWPNRQPIPF 378
           KYFQKIESWPNRQP+PF
Sbjct: 361 KYFQKIESWPNRQPVPF 293

[Close]

BLAST of Spg031349 vs. NCBI nr
Match: XP_008438171.1 (PREDICTED: tRNA 2'-phosphotransferase 1 [Cucumis melo])

HSP 1 Score: 485.7 bits (1249), Expect = 3.6e-133
Identity = 266/377 (70.56%), Postives = 280/377 (74.27%), Query Frame = 0


Query: 1   MWASVVTNCGIKILRYRPLLLPRVLIAPIPLPIAPPVSSMDRSKASSFSSSAHSSKSGGR 60
           MWASVVTNCGIKILR R LLLPRVLI PIPLPI+P + SMDRS  +S SS AHSSKSGGR
Sbjct: 1   MWASVVTNCGIKILRCRSLLLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 61  GRGLDMRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQD 120
           GRGLD+RNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMR+DGYVKV+D
Sbjct: 61  GRGLDLRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRNDGYVKVED 120

Query: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEEDGELLIRANQGHTVTVVETES 180
           LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRF LL+E+GELLIRANQGHTVT VETES
Sbjct: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLDENGELLIRANQGHTVTAVETES 180

Query: 181 LLKPILSAEEVPVCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGKLRKSY 240
           LLKPILSAEEVPVCVHGTYKKNLESIL+SGLKRMKRLHVHFSCGLPTDGEVISG      
Sbjct: 181 LLKPILSAEEVPVCVHGTYKKNLESILDSGLKRMKRLHVHFSCGLPTDGEVISG------ 240

Query: 241 CYISLKNLSDKTTMPLCLAINCFCNCTSSPTYLFISGKPGRTVRSANQFEVGLLDVESWR 300
                                                                       
Sbjct: 241 ------------------------------------------------------------ 293

Query: 301 ILRYMYLEGERERVGGSCMRRDVNVLIFLDVKKALDDGMKLYISDNKVILTEGFDGVVVV 360
                             MRRDVNVLIFLDV+KAL+DGMKLYISDN+VILTEGFDG+V +
Sbjct: 301 ------------------MRRDVNVLIFLDVQKALEDGMKLYISDNRVILTEGFDGMVPI 293

Query: 361 KYFQKIESWPNRQPIPF 378
           KYFQKIESWPNRQPI F
Sbjct: 361 KYFQKIESWPNRQPISF 293

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BLAST of Spg031349 vs. ExPASy Swiss-Prot
Match: Q5EAR5 (tRNA 2'-phosphotransferase 1 OS=Danio rerio OX=7955 GN=trpt1 PE=2 SV=2)

HSP 1 Score: 110.9 bits (276), Expect = 3.1e-23
Identity = 70/166 (42.17%), Postives = 98/166 (59.04%), Query Frame = 0


Query: 72  RTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQDLLKLNLKTFAN 131
           R RGR G G  + + +   L + L+ +LRH AS++ L M SDG+V V++LL       A+
Sbjct: 6   RGRGRRGRGNRNEESRDVRLSKSLSYVLRHGASKMGLQMNSDGFVFVEELL-------AH 65

Query: 132 IPLRSHTIDDIREAVRKDNKQRFGLLE--EDGELLIRANQGHTVTVVETESLLKPILSAE 191
              RS ++DD+   V  ++KQRF L +  ED  L IRANQGH+V V + E L +     +
Sbjct: 66  QQFRSFSVDDVERVVASNDKQRFKLCKHPEDDRLQIRANQGHSVQVTDLE-LREISQDDQ 125

Query: 192 EVP-VCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISG 235
           + P   VHG+Y K+  SI   GL RM R H+H + GLP +G VISG
Sbjct: 126 DYPREAVHGSYMKHWPSIRSQGLSRMNRTHIHLAPGLPGEGRVISG 163

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BLAST of Spg031349 vs. ExPASy Swiss-Prot
Match: O14045 (Putative tRNA 2'-phosphotransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC2C4.12c PE=3 SV=2)

HSP 1 Score: 103.2 bits (256), Expect = 6.5e-21
Identity = 79/276 (28.62%), Postives = 120/276 (43.48%), Query Frame = 0


Query: 93  RLLTRILRHMASELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHTIDDIREAVRKDNKQ 152
           + L+++LRH A    L +R DGY++V  +LKL          R   ++ +   V+ ++K+
Sbjct: 43  KALSKVLRHTAKANGLQIREDGYIEVDSILKLP-------QFRGMGMELLLSIVKGNDKK 102

Query: 153 RFGLLEEDGELLIRANQGHTVTVVETESLLKPILSAEEVPVCVHGTYKKNLESILESGLK 212
           RF + E +G L IRANQGH++  V+    +  I +A  +P  VHGT K+    I + GL 
Sbjct: 103 RFTMEEVEGVLYIRANQGHSIKAVQVP--MARIDNASSIPKVVHGTKKELWPVISKQGLS 162

Query: 213 RMKRLHVHFSCGLPTDGEVISGKLRKSYCYISLKNLSDKTTMPLCLAINCFCNCTSSPTY 272
           RMKR H+H + GL  D  VISG +RKS                          CT     
Sbjct: 163 RMKRNHIHCATGLYGDPGVISG-IRKS--------------------------CT----- 222

Query: 273 LFISGKPGRTVRSANQFEVGLLDVESWRILRYMYLEGERERVGGSCMRRDVNVLIFLDVK 332
                                                               + I++D  
Sbjct: 223 ----------------------------------------------------LYIYIDSA 225

Query: 333 KALDDGMKLYISDNKVILTEGFDGVVVVKYFQKIES 369
           KA+ DG++ Y S+N VILTEG +G++  KYF ++E+
Sbjct: 283 KAMQDGVEFYRSENGVILTEGVNGLLSSKYFSRVET 225

[Close]

BLAST of Spg031349 vs. ExPASy Swiss-Prot
Match: Q8K3A2 (tRNA 2'-phosphotransferase 1 OS=Mus musculus OX=10090 GN=Trpt1 PE=2 SV=2)

HSP 1 Score: 100.1 bits (248), Expect = 5.5e-20
Identity = 65/153 (42.48%), Postives = 90/153 (58.82%), Query Frame = 0


Query: 85  KDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHTIDDIRE 144
           +D+   L + L+  LRH A +L L MR+DG+V +Q LL+L           S +I+D++ 
Sbjct: 20  QDRNVQLSKALSYALRHGALKLGLPMRADGFVPLQALLQLP-------QFHSFSIEDVQL 79

Query: 145 AVRKDNKQRFGLL--EEDGELLIRANQGHTVTVVETESLLKPILSAEEVPV-CVHGTYKK 204
            V  + KQRF L   E    LLIRANQGH++ V E E  L P+ + + +P+  VHGT+ K
Sbjct: 80  VVNTNEKQRFTLQPGEPSTGLLIRANQGHSLQVPELE--LTPLETPQALPLTLVHGTFWK 139

Query: 205 NLESILESGLKRMKRLHVHFSCGLPTDGEVISG 235
           +  SIL  GL R  R H+H + GLP D  VISG
Sbjct: 140 HWPSILLKGLSRQGRTHIHLASGLPGDPGVISG 163

[Close]

BLAST of Spg031349 vs. ExPASy Swiss-Prot
Match: Q3ZBM7 (tRNA 2'-phosphotransferase 1 OS=Bos taurus OX=9913 GN=TRPT1 PE=2 SV=3)

HSP 1 Score: 86.7 bits (213), Expect = 6.3e-16
Identity = 63/171 (36.84%), Postives = 93/171 (54.39%), Query Frame = 0


Query: 67  RNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQDLLKLNL 126
           R +    +GR        +D+   L + L+  LRH A +L L M +DG+V +  LL+L  
Sbjct: 8   RRETAGPKGRRAHRPPQDQDRDVQLSKALSYALRHGALKLGLPMGADGFVPLDALLQLP- 67

Query: 127 KTFANIPLRSHTIDDIREAVRKDNKQRFGLLEEDGEL--LIRANQGHTVTVVETESLLKP 186
                   RS + +D++  V  + KQRF L   D     LIRANQGH++ V E E  L+P
Sbjct: 68  ------QFRSFSAEDVQRVVDTNVKQRFALQPGDPSTGPLIRANQGHSLQVPELE--LEP 127

Query: 187 ILSAEEVP-VCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISG 235
           + + + +P + VHGT++++  SIL  GL    R H+H + GLP D  VISG
Sbjct: 128 LETPQALPLMLVHGTFRQHWPSILLKGLSCRGRTHIHLAPGLPGDPGVISG 169

[Close]

BLAST of Spg031349 vs. ExPASy Swiss-Prot
Match: Q8XP22 (Probable RNA 2'-phosphotransferase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=kptA PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.1e-12
Identity = 47/143 (32.87%), Postives = 79/143 (55.24%), Query Frame = 0


Query: 85  KDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHTIDDIRE 144
           K+    + + ++ ILRH   E+ L +   GY+ V DL++   K++     +  ++DD+  
Sbjct: 2   KNNDSKISKYISLILRHKPEEIGLKLDEHGYLGVLDLIEGLNKSY-----KGFSMDDLER 61

Query: 145 AVRKDNKQRFGLLEEDGELLIRANQGHTVTVVETESLLKPILSAEEVPVCVHGTYKKNLE 204
            VR+D+K R+   E+  +  IRANQGH++ V      +KP        V  HGT +K +E
Sbjct: 62  IVREDSKGRYSFNEDKSK--IRANQGHSIKVDLGLEAIKP------PKVLYHGTGRKYIE 121

Query: 205 SILESGLKRMKRLHVHFSCGLPT 228
           SIL++GL + +R +VH S  + T
Sbjct: 122 SILKNGLIKKERQYVHLSKDIET 131

[Close]

BLAST of Spg031349 vs. ExPASy TrEMBL
Match: A0A6J1CZ57 (2'-phosphotransferase OS=Momordica charantia OX=3673 GN=LOC111016108 PE=3 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 2.2e-136
Identity = 274/377 (72.68%), Postives = 280/377 (74.27%), Query Frame = 0


Query: 1   MWASVVTNCGIKILRYRPLLLPRVLIAPIPLPIAPPVSSMDRSKASSFSSSAHSSKSGGR 60
           MWA  VTNCGIKILRYRPLLLPRVLI PIPLPI P V SMD    SSF+S AHSSKSGGR
Sbjct: 1   MWA-CVTNCGIKILRYRPLLLPRVLITPIPLPIPPAVFSMDNPNTSSFASFAHSSKSGGR 60

Query: 61  GRGLDMRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQD 120
           GRGLDM NDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQD
Sbjct: 61  GRGLDMTNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQD 120

Query: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEEDGELLIRANQGHTVTVVETES 180
           LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEE+GELLIRANQGHTVTVVETES
Sbjct: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEENGELLIRANQGHTVTVVETES 180

Query: 181 LLKPILSAEEVPVCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGKLRKSY 240
           LL+PILSAEEVPVCVHG+YKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISG      
Sbjct: 181 LLRPILSAEEVPVCVHGSYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISG------ 240

Query: 241 CYISLKNLSDKTTMPLCLAINCFCNCTSSPTYLFISGKPGRTVRSANQFEVGLLDVESWR 300
                                                                       
Sbjct: 241 ------------------------------------------------------------ 292

Query: 301 ILRYMYLEGERERVGGSCMRRDVNVLIFLDVKKALDDGMKLYISDNKVILTEGFDGVVVV 360
                             MRRDVNVLIFLDVKKALDDGMKLY+SDNKVILTEGFDGVV V
Sbjct: 301 ------------------MRRDVNVLIFLDVKKALDDGMKLYVSDNKVILTEGFDGVVAV 292

Query: 361 KYFQKIESWPNRQPIPF 378
           KYFQKIESWP+RQPIPF
Sbjct: 361 KYFQKIESWPSRQPIPF 292

[Close]

BLAST of Spg031349 vs. ExPASy TrEMBL
Match: A0A5D3D3K0 (2'-phosphotransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003870 PE=3 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.7e-134
Identity = 267/377 (70.82%), Postives = 280/377 (74.27%), Query Frame = 0


Query: 1   MWASVVTNCGIKILRYRPLLLPRVLIAPIPLPIAPPVSSMDRSKASSFSSSAHSSKSGGR 60
           MWASVVTNCGIKILR R LLLPRVLI PIPLPI+P + SMDRS  +S SS AHSSKSGGR
Sbjct: 1   MWASVVTNCGIKILRCRSLLLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 61  GRGLDMRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQD 120
           GRGLD+RNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMR+DGYVKV+D
Sbjct: 61  GRGLDLRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRNDGYVKVED 120

Query: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEEDGELLIRANQGHTVTVVETES 180
           LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRF LL+E+GELLIRANQGHTVT VETES
Sbjct: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLDENGELLIRANQGHTVTAVETES 180

Query: 181 LLKPILSAEEVPVCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGKLRKSY 240
           LLKPILSAEEVPVCVHGTYKKNLESIL+SGLKRMKRLHVHFSCGLPTDGEVISG      
Sbjct: 181 LLKPILSAEEVPVCVHGTYKKNLESILDSGLKRMKRLHVHFSCGLPTDGEVISG------ 240

Query: 241 CYISLKNLSDKTTMPLCLAINCFCNCTSSPTYLFISGKPGRTVRSANQFEVGLLDVESWR 300
                                                                       
Sbjct: 241 ------------------------------------------------------------ 293

Query: 301 ILRYMYLEGERERVGGSCMRRDVNVLIFLDVKKALDDGMKLYISDNKVILTEGFDGVVVV 360
                             MRRDVNVLIFLDV+KAL+DGMKLYISDN+VILTEGFDG V +
Sbjct: 301 ------------------MRRDVNVLIFLDVQKALEDGMKLYISDNRVILTEGFDGTVPI 293

Query: 361 KYFQKIESWPNRQPIPF 378
           KYFQKIESWPNRQPIPF
Sbjct: 361 KYFQKIESWPNRQPIPF 293

[Close]

BLAST of Spg031349 vs. ExPASy TrEMBL
Match: A0A0A0L3T7 (2'-phosphotransferase OS=Cucumis sativus OX=3659 GN=Csa_3G126200 PE=3 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.3e-133
Identity = 265/377 (70.29%), Postives = 279/377 (74.01%), Query Frame = 0


Query: 1   MWASVVTNCGIKILRYRPLLLPRVLIAPIPLPIAPPVSSMDRSKASSFSSSAHSSKSGGR 60
           MWASVVTNCGIKILR RPL LPRVLI PIPLPI+P + SMDRS  +S SS AHSSKSGGR
Sbjct: 1   MWASVVTNCGIKILRCRPLWLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 61  GRGLDMRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQD 120
           GR LD+RNDRERTRGRGGGGGGSGKDKIDALGRLLTR+LRHMASELNLNMRSDGYVKV+D
Sbjct: 61  GRDLDLRNDRERTRGRGGGGGGSGKDKIDALGRLLTRVLRHMASELNLNMRSDGYVKVED 120

Query: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEEDGELLIRANQGHTVTVVETES 180
           LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRF  L+E+GELLIRANQGHTVT VETES
Sbjct: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSFLDENGELLIRANQGHTVTAVETES 180

Query: 181 LLKPILSAEEVPVCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGKLRKSY 240
           LLKPILSAEEVPVCVHGTYKKNLESIL+SGLKRMKRLHVHFSCGLPTDGEVISG      
Sbjct: 181 LLKPILSAEEVPVCVHGTYKKNLESILDSGLKRMKRLHVHFSCGLPTDGEVISG------ 240

Query: 241 CYISLKNLSDKTTMPLCLAINCFCNCTSSPTYLFISGKPGRTVRSANQFEVGLLDVESWR 300
                                                                       
Sbjct: 241 ------------------------------------------------------------ 293

Query: 301 ILRYMYLEGERERVGGSCMRRDVNVLIFLDVKKALDDGMKLYISDNKVILTEGFDGVVVV 360
                             MRRDVNVLIFLDV+KAL+DGMKLYISDN+VILTEGFDGVV +
Sbjct: 301 ------------------MRRDVNVLIFLDVQKALEDGMKLYISDNRVILTEGFDGVVPM 293

Query: 361 KYFQKIESWPNRQPIPF 378
           KYFQKIESWPNRQP+PF
Sbjct: 361 KYFQKIESWPNRQPVPF 293

[Close]

BLAST of Spg031349 vs. ExPASy TrEMBL
Match: A0A1S3AVU4 (2'-phosphotransferase OS=Cucumis melo OX=3656 GN=LOC103483358 PE=3 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.7e-133
Identity = 266/377 (70.56%), Postives = 280/377 (74.27%), Query Frame = 0


Query: 1   MWASVVTNCGIKILRYRPLLLPRVLIAPIPLPIAPPVSSMDRSKASSFSSSAHSSKSGGR 60
           MWASVVTNCGIKILR R LLLPRVLI PIPLPI+P + SMDRS  +S SS AHSSKSGGR
Sbjct: 1   MWASVVTNCGIKILRCRSLLLPRVLITPIPLPISPSLLSMDRSNTTSSSSFAHSSKSGGR 60

Query: 61  GRGLDMRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQD 120
           GRGLD+RNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMR+DGYVKV+D
Sbjct: 61  GRGLDLRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRNDGYVKVED 120

Query: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEEDGELLIRANQGHTVTVVETES 180
           LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRF LL+E+GELLIRANQGHTVT VETES
Sbjct: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFSLLDENGELLIRANQGHTVTAVETES 180

Query: 181 LLKPILSAEEVPVCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGKLRKSY 240
           LLKPILSAEEVPVCVHGTYKKNLESIL+SGLKRMKRLHVHFSCGLPTDGEVISG      
Sbjct: 181 LLKPILSAEEVPVCVHGTYKKNLESILDSGLKRMKRLHVHFSCGLPTDGEVISG------ 240

Query: 241 CYISLKNLSDKTTMPLCLAINCFCNCTSSPTYLFISGKPGRTVRSANQFEVGLLDVESWR 300
                                                                       
Sbjct: 241 ------------------------------------------------------------ 293

Query: 301 ILRYMYLEGERERVGGSCMRRDVNVLIFLDVKKALDDGMKLYISDNKVILTEGFDGVVVV 360
                             MRRDVNVLIFLDV+KAL+DGMKLYISDN+VILTEGFDG+V +
Sbjct: 301 ------------------MRRDVNVLIFLDVQKALEDGMKLYISDNRVILTEGFDGMVPI 293

Query: 361 KYFQKIESWPNRQPIPF 378
           KYFQKIESWPNRQPI F
Sbjct: 361 KYFQKIESWPNRQPISF 293

[Close]

BLAST of Spg031349 vs. ExPASy TrEMBL
Match: A0A6J1I9U5 (2'-phosphotransferase OS=Cucurbita maxima OX=3661 GN=LOC111472467 PE=3 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 1.0e-125
Identity = 257/377 (68.17%), Postives = 268/377 (71.09%), Query Frame = 0


Query: 1   MWASVVTNCGIKILRYRPLLLPRVLIAPIPLPIAPPVSSMDRSKASSFSSSAHSSKSGGR 60
           MWASVVT CGIK +R RPLLLPR         I+P + SMDRS  SSFSS AHS+KSGGR
Sbjct: 1   MWASVVTICGIKFMRCRPLLLPR---------ISPSLFSMDRSNTSSFSSFAHSTKSGGR 60

Query: 61  GRGLDMRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQD 120
           GRGLDMR+DRE TRGRGGGG GSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVK+QD
Sbjct: 61  GRGLDMRSDRESTRGRGGGGDGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKLQD 120

Query: 121 LLKLNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEEDGELLIRANQGHTVTVVETES 180
           LLKLNLKTFANIPLRSHT+DDIREAVRKDNKQRF LLEE+GEL IRANQGHTVTVVETES
Sbjct: 121 LLKLNLKTFANIPLRSHTVDDIREAVRKDNKQRFSLLEENGELFIRANQGHTVTVVETES 180

Query: 181 LLKPILSAEEVPVCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGKLRKSY 240
           LLKPILSAEEVPVCVHGTYK NLESIL SGLKRMKRLHVHFSCGLPTDGEVISG      
Sbjct: 181 LLKPILSAEEVPVCVHGTYKTNLESILASGLKRMKRLHVHFSCGLPTDGEVISG------ 240

Query: 241 CYISLKNLSDKTTMPLCLAINCFCNCTSSPTYLFISGKPGRTVRSANQFEVGLLDVESWR 300
                                                                       
Sbjct: 241 ------------------------------------------------------------ 284

Query: 301 ILRYMYLEGERERVGGSCMRRDVNVLIFLDVKKALDDGMKLYISDNKVILTEGFDGVVVV 360
                             MRR+VNVLIFLDV KAL+DGMKLY+SDNKVILTEGFDGVV V
Sbjct: 301 ------------------MRRNVNVLIFLDVNKALNDGMKLYMSDNKVILTEGFDGVVAV 284

Query: 361 KYFQKIESWPNRQPIPF 378
           KYFQKIESWPNRQPIPF
Sbjct: 361 KYFQKIESWPNRQPIPF 284

[Close]

BLAST of Spg031349 vs. TAIR 10
Match: AT2G45330.1 (RNA 2'-phosphotransferase, Tpt1 / KptA family )

HSP 1 Score: 346.3 bits (887), Expect = 3.1e-95
Identity = 203/374 (54.28%), Postives = 238/374 (63.64%), Query Frame = 0


Query: 11  IKILRYRPLLLPRVLIAPIPLPIAPPVSSM-------DRSKASSFSSSAHSSKSGGRGRG 70
           +++ R+R L LP  L + +    + PVSS        + S+ S+ SS A SS+SGGRG G
Sbjct: 3   LRLCRHR-LPLPLTLSSSVFFSKSSPVSSAFMDASNPNSSRKSNVSSFAQSSRSGGRGGG 62

Query: 71  LDMRNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQDLLK 130
            +  NDR R +GRG GGG  GKD+IDALGRLLTRILRHMA+EL LNMR DG+VKV+DLL 
Sbjct: 63  YERDNDRRRPQGRGDGGG--GKDRIDALGRLLTRILRHMATELRLNMRGDGFVKVEDLLN 122

Query: 131 LNLKTFANIPLRSHTIDDIREAVRKDNKQRFGLLEEDGELLIRANQGHTVTVVETESLLK 190
           LNLKT ANI L+SHTID+IREAVR+DNKQRF L++E+GELLIRANQGH++T VE+E LLK
Sbjct: 123 LNLKTSANIQLKSHTIDEIREAVRRDNKQRFSLIDENGELLIRANQGHSITTVESEKLLK 182

Query: 191 PILSAEEVPVCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGKLRKSYCYI 250
           PILS EE PVCVHGTY+KNLESIL SGLKRM R+HVHFSCGLPTDGEVISG         
Sbjct: 183 PILSPEEAPVCVHGTYRKNLESILASGLKRMNRMHVHFSCGLPTDGEVISG--------- 242

Query: 251 SLKNLSDKTTMPLCLAINCFCNCTSSPTYLFISGKPGRTVRSANQFEVGLLDVESWRILR 310
                                                                       
Sbjct: 243 ------------------------------------------------------------ 289

Query: 311 YMYLEGERERVGGSCMRRDVNVLIFLDVKKALDDGMKLYISDNKVILTEGFDGVVVVKYF 370
                          MRR+VNV+IFLD+KKAL+DG+  YISDNKVILTEG DGV+ V YF
Sbjct: 303 ---------------MRRNVNVIIFLDIKKALEDGIAFYISDNKVILTEGIDGVLPVDYF 289

Query: 371 QKIESWPNRQPIPF 378
           QKIESWP+RQ IPF
Sbjct: 363 QKIESWPDRQSIPF 289

[Close]

BLAST of Spg031349 vs. TAIR 10
Match: AT2G45330.2 (RNA 2'-phosphotransferase, Tpt1 / KptA family )

HSP 1 Score: 335.9 bits (860), Expect = 4.2e-92
Identity = 201/371 (54.18%), Postives = 234/371 (63.07%), Query Frame = 0


Query: 11  IKILRYRPLLLPRVLIAPIPLPIAPPVSS--MDRSKASSFSSSAHSS--KSGGRGRGLDM 70
           +++ R+R L LP  L + +    + PVSS  MD S  +S   S  SS  +S  RG G + 
Sbjct: 3   LRLCRHR-LPLPLTLSSSVFFSKSSPVSSAFMDASNPNSSRKSNVSSFAQSSRRGGGYER 62

Query: 71  RNDRERTRGRGGGGGGSGKDKIDALGRLLTRILRHMASELNLNMRSDGYVKVQDLLKLNL 130
            NDR R +GRG GGG  GKD+IDALGRLLTRILRHMA+EL LNMR DG+VKV+DLL LNL
Sbjct: 63  DNDRRRPQGRGDGGG--GKDRIDALGRLLTRILRHMATELRLNMRGDGFVKVEDLLNLNL 122

Query: 131 KTFANIPLRSHTIDDIREAVRKDNKQRFGLLEEDGELLIRANQGHTVTVVETESLLKPIL 190
           KT ANI L+SHTID+IREAVR+DNKQRF L++E+GELLIRANQGH++T VE+E LLKPIL
Sbjct: 123 KTSANIQLKSHTIDEIREAVRRDNKQRFSLIDENGELLIRANQGHSITTVESEKLLKPIL 182

Query: 191 SAEEVPVCVHGTYKKNLESILESGLKRMKRLHVHFSCGLPTDGEVISGKLRKSYCYISLK 250
           S EE PVCVHGTY+KNLESIL SGLKRM R+HVHFSCGLPTDGEVISG            
Sbjct: 183 SPEEAPVCVHGTYRKNLESILASGLKRMNRMHVHFSCGLPTDGEVISG------------ 242

Query: 251 NLSDKTTMPLCLAINCFCNCTSSPTYLFISGKPGRTVRSANQFEVGLLDVESWRILRYMY 310
                                                                       
Sbjct: 243 ------------------------------------------------------------ 286

Query: 311 LEGERERVGGSCMRRDVNVLIFLDVKKALDDGMKLYISDNKVILTEGFDGVVVVKYFQKI 370
                       MRR+VNV+IFLD+KKAL+DG+  YISDNKVILTEG DGV+ V YFQKI
Sbjct: 303 ------------MRRNVNVIIFLDIKKALEDGIAFYISDNKVILTEGIDGVLPVDYFQKI 286

Query: 371 ESWPNRQPIPF 378
           ESWP+RQ IPF
Sbjct: 363 ESWPDRQSIPF 286

[Close]

BLAST of Spg031349 vs. TAIR 10
Match: AT5G23600.1 (RNA 2'-phosphotransferase, Tpt1 / KptA family )

HSP 1 Score: 301.6 bits (771), Expect = 8.9e-82
Identity = 162/292 (55.48%), Postives = 186/292 (63.70%), Query Frame = 0


Query: 86  DKIDALGRLLTRILRHMASELNLNMRSDGYVKVQDLLKLNLKTFANIPLRSHTIDDIREA 145
           D+IDALGR+LTRILRHMA+EL LNMR DG+VKV+DLL LNLKT ANI L SHTID+IREA
Sbjct: 5   DRIDALGRILTRILRHMATELRLNMRGDGFVKVEDLLNLNLKTCANIQLNSHTIDEIREA 64

Query: 146 VRKDNKQRFGLLEEDGELLIRANQGHTVTVVETESLLKPILSAEEVPVCVHGTYKKNLES 205
           V +DNK+RF L++EDGELLIRANQGH++T VE+E LLKPILS EE PVCVHGTY+KNLES
Sbjct: 65  VTRDNKKRFSLIDEDGELLIRANQGHSITTVESEKLLKPILSPEEAPVCVHGTYRKNLES 124

Query: 206 ILESGLKRMKRLHVHFSCGLPTDGEVISGKLRKSYCYISLKNLSDKTTMPLCLAINCFCN 265
           IL SGLKRM R+HVHFSCGLPTDGEVISG                               
Sbjct: 125 ILASGLKRMNRMHVHFSCGLPTDGEVISG------------------------------- 184

Query: 266 CTSSPTYLFISGKPGRTVRSANQFEVGLLDVESWRILRYMYLEGERERVGGSCMRRDVNV 325
                                                                +RR+VNV
Sbjct: 185 -----------------------------------------------------VRRNVNV 212

Query: 326 LIFLDVKKALDDGMKLYISDNKVILTEGFDGVVVVKYFQKIESWPNRQPIPF 378
           +IFL +KKAL+DG+  YISDNKVILT+G  GV+ V YFQKIESWP+RQ IPF
Sbjct: 245 IIFLHIKKALEDGIAFYISDNKVILTQGIVGVLPVDYFQKIESWPDRQSIPF 212

The following BLAST results are available for this feature:

BLAST of Spg031349 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147085.1	4.5e-136	72.68	tRNA 2'-phosphotransferase 1 [Momordica charantia] >XP_022147086.1 tRNA 2'-phosp...	[more]
XP_038893116.1	7.7e-136	71.88	tRNA 2'-phosphotransferase 1 [Benincasa hispida]	[more]
TYK17519.1	5.5e-134	70.82	tRNA 2'-phosphotransferase 1 [Cucumis melo var. makuwa]	[more]
XP_004133917.2	2.7e-133	70.29	tRNA 2'-phosphotransferase 1 isoform X1 [Cucumis sativus] >KGN56605.1 hypothetic...	[more]
XP_008438171.1	3.6e-133	70.56	PREDICTED: tRNA 2'-phosphotransferase 1 [Cucumis melo]	[more]

BLAST of Spg031349 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5EAR5	3.1e-23	42.17	tRNA 2'-phosphotransferase 1 OS=Danio rerio OX=7955 GN=trpt1 PE=2 SV=2	[more]
O14045	6.5e-21	28.62	Putative tRNA 2'-phosphotransferase OS=Schizosaccharomyces pombe (strain 972 / A...	[more]
Q8K3A2	5.5e-20	42.48	tRNA 2'-phosphotransferase 1 OS=Mus musculus OX=10090 GN=Trpt1 PE=2 SV=2	[more]
Q3ZBM7	6.3e-16	36.84	tRNA 2'-phosphotransferase 1 OS=Bos taurus OX=9913 GN=TRPT1 PE=2 SV=3	[more]
Q8XP22	1.1e-12	32.87	Probable RNA 2'-phosphotransferase OS=Clostridium perfringens (strain 13 / Type ...	[more]

BLAST of Spg031349 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CZ57	2.2e-136	72.68	2'-phosphotransferase OS=Momordica charantia OX=3673 GN=LOC111016108 PE=3 SV=1	[more]
A0A5D3D3K0	2.7e-134	70.82	2'-phosphotransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold43...	[more]
A0A0A0L3T7	1.3e-133	70.29	2'-phosphotransferase OS=Cucumis sativus OX=3659 GN=Csa_3G126200 PE=3 SV=1	[more]
A0A1S3AVU4	1.7e-133	70.56	2'-phosphotransferase OS=Cucumis melo OX=3656 GN=LOC103483358 PE=3 SV=1	[more]
A0A6J1I9U5	1.0e-125	68.17	2'-phosphotransferase OS=Cucurbita maxima OX=3661 GN=LOC111472467 PE=3 SV=1	[more]

BLAST of Spg031349 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45330.1	3.1e-95	54.28	RNA 2'-phosphotransferase, Tpt1 / KptA family	[more]
AT2G45330.2	4.2e-92	54.18	RNA 2'-phosphotransferase, Tpt1 / KptA family	[more]
AT5G23600.1	8.9e-82	55.48	RNA 2'-phosphotransferase, Tpt1 / KptA family	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002745	Phosphotransferase KptA/Tpt1	PFAM	PF01885	PTS_2-RNA	coord: 317..353 e-value: 1.5E-9 score: 38.2 coord: 89..245 e-value: 7.2E-47 score: 159.8
IPR002745	Phosphotransferase KptA/Tpt1	PANTHER	PTHR12684	PUTATIVE PHOSPHOTRANSFERASE	coord: 317..377 coord: 68..245
IPR042081	RNA 2'-phosphotransferase, C-terminal domain	GENE3D	3.20.170.30		coord: 193..256 e-value: 7.1E-12 score: 47.1 coord: 292..372 e-value: 1.1E-8 score: 37.0
IPR042080	RNA 2'-phosphotransferase, N-terminal domain	GENE3D	1.10.10.970		coord: 87..167 e-value: 8.2E-18 score: 66.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 60..76
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 40..85
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 40..57
None	No IPR available	SUPERFAMILY	56399	ADP-ribosylation	coord: 91..243
None	No IPR available	SUPERFAMILY	56399	ADP-ribosylation	coord: 316..359

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg031349.1	Spg031349.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006388	tRNA splicing, via endonucleolytic cleavage and ligation
molecular_function	GO:0000215	tRNA 2'-phosphotransferase activity
molecular_function	GO:0016740	transferase activity