Spg031305 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg031305
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationscaffold11: 40283832 .. 40284873 (+)
RNA-Seq ExpressionSpg031305
SyntenySpg031305
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGTCGAAGCCTCAAGAGTCAATACGCTCAGCCTCACTCCGATATCCCAAGCCGACGAAAACTACCACAGATCCACTGACCTCAAAGCTTTCGATGACACTAAATCCGGCGTTAAAGGCTTAGTCGACGCCGGAATTACTGAAATTCCTCGCATCTTTTACAAGCCACCCGATGAAGATCACTCCGGCGATGTTTCCGGCGAAACCCAACTCCATATTCCAGTGATAGACCTCAACCACGTCGGCAGAAATTCACTCAAACGCAAGTACACCATCGACAGAATCCGAGGAGCTTCAGAGAAATTGGGGTTTTTCCAACTGATCAACCATGGGATTCCGGTGAGCGTTCTTGAAGAGATGAAAGATGCGGTTCGGAGGTTTCACGAACAGGACACAGAATTGAAGAAACAATACTATACCCGCAACGTCACGAAGCCCTTGATTTACACCAGTAATTTCGATCTCTTTTCTGCTGCGACCACCAATTGGAGGGATACGTTTCGGTATACAAGTGCCCCAAATTCCCACGATCCGCAAGACCTCCCAGAAATTTGCAGGTAAATGTGCAGATTTTGGACCCAAAAAAGAAAACCCTTTTTAAACAGAGATTCCTGTTTAAACTTACCTCTGTTTGTGCAGAGACATTCTAGTGGATTACTCGAAGCGAGTGATGGAAATTGGGAAATTACTGTTTCAATTGCTGTCGGAGGCTCTGGGCCTGGATTCAAATTACTTGAACAATATAGACTGCAGCGAAGGGCTTGCACTTCTTTGCCACTATTACCCACCATGCCCACAGCCGAATTTGACCATCGGCACATCGGAGCACACAGACAATACCTTTATCACTGTTCTGTTGCAAGACCACATCGGCGGCCTACAGATTCGCCATGAGAACAATTGGGTCGACGTTCCCCCTGTCGCCGGAGCTTTAGTCGTGAACATCGGAGATCTTATGCAGGCAAGTTTCTGCAAAATTTCTTTCTCTGCCAATGATGAATACCCTCTAATTCGGTTGGGTTTTGGATGCAGCTTATAA

mRNA sequence

ATGGCAGTCGAAGCCTCAAGAGTCAATACGCTCAGCCTCACTCCGATATCCCAAGCCGACGAAAACTACCACAGATCCACTGACCTCAAAGCTTTCGATGACACTAAATCCGGCGTTAAAGGCTTAGTCGACGCCGGAATTACTGAAATTCCTCGCATCTTTTACAAGCCACCCGATGAAGATCACTCCGGCGATGTTTCCGGCGAAACCCAACTCCATATTCCAGTGATAGACCTCAACCACGTCGGCAGAAATTCACTCAAACGCAAGTACACCATCGACAGAATCCGAGGAGCTTCAGAGAAATTGGGGTTTTTCCAACTGATCAACCATGGGATTCCGGTGAGCGTTCTTGAAGAGATGAAAGATGCGGTTCGGAGGTTTCACGAACAGGACACAGAATTGAAGAAACAATACTATACCCGCAACGTCACGAAGCCCTTGATTTACACCAGTAATTTCGATCTCTTTTCTGCTGCGACCACCAATTGGAGGGATACGTTTCGGTATACAAGTGCCCCAAATTCCCACGATCCGCAAGACCTCCCAGAAATTTGCAGAGACATTCTAGTGGATTACTCGAAGCGAGTGATGGAAATTGGGAAATTACTGTTTCAATTGCTGTCGGAGGCTCTGGGCCTGGATTCAAATTACTTGAACAATATAGACTGCAGCGAAGGGCTTGCACTTCTTTGCCACTATTACCCACCATGCCCACAGCCGAATTTGACCATCGGCACATCGGAGCACACAGACAATACCTTTATCACTGTTCTGTTGCAAGACCACATCGGCGGCCTACAGATTCGCCATGAGAACAATTGGGTCGACGTTCCCCCTGTCGCCGGAGCTTTAGTCGTGAACATCGGAGATCTTATGCAGGCAAGTTTCTGCAAAATTTCTTTCTCTGCCAATGATGAATACCCTCTAATTCGGTTGGGTTTTGGATGCAGCTTATAA

Coding sequence (CDS)

ATGGCAGTCGAAGCCTCAAGAGTCAATACGCTCAGCCTCACTCCGATATCCCAAGCCGACGAAAACTACCACAGATCCACTGACCTCAAAGCTTTCGATGACACTAAATCCGGCGTTAAAGGCTTAGTCGACGCCGGAATTACTGAAATTCCTCGCATCTTTTACAAGCCACCCGATGAAGATCACTCCGGCGATGTTTCCGGCGAAACCCAACTCCATATTCCAGTGATAGACCTCAACCACGTCGGCAGAAATTCACTCAAACGCAAGTACACCATCGACAGAATCCGAGGAGCTTCAGAGAAATTGGGGTTTTTCCAACTGATCAACCATGGGATTCCGGTGAGCGTTCTTGAAGAGATGAAAGATGCGGTTCGGAGGTTTCACGAACAGGACACAGAATTGAAGAAACAATACTATACCCGCAACGTCACGAAGCCCTTGATTTACACCAGTAATTTCGATCTCTTTTCTGCTGCGACCACCAATTGGAGGGATACGTTTCGGTATACAAGTGCCCCAAATTCCCACGATCCGCAAGACCTCCCAGAAATTTGCAGAGACATTCTAGTGGATTACTCGAAGCGAGTGATGGAAATTGGGAAATTACTGTTTCAATTGCTGTCGGAGGCTCTGGGCCTGGATTCAAATTACTTGAACAATATAGACTGCAGCGAAGGGCTTGCACTTCTTTGCCACTATTACCCACCATGCCCACAGCCGAATTTGACCATCGGCACATCGGAGCACACAGACAATACCTTTATCACTGTTCTGTTGCAAGACCACATCGGCGGCCTACAGATTCGCCATGAGAACAATTGGGTCGACGTTCCCCCTGTCGCCGGAGCTTTAGTCGTGAACATCGGAGATCTTATGCAGGCAAGTTTCTGCAAAATTTCTTTCTCTGCCAATGATGAATACCCTCTAATTCGGTTGGGTTTTGGATGCAGCTTATAA

Protein sequence

MAVEASRVNTLSLTPISQADENYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDEDHSGDVSGETQLHIPVIDLNHVGRNSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEEMKDAVRRFHEQDTELKKQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQDLPEICRDILVDYSKRVMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQPNLTIGTSEHTDNTFITVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQASFCKISFSANDEYPLIRLGFGCSL
Homology
BLAST of Spg031305 vs. NCBI nr
Match: KAG6597376.1 (1-aminocyclopropane-1-carboxylate oxidase-like 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 520.8 bits (1340), Expect = 8.4e-144
Identity = 246/301 (81.73%), Postives = 274/301 (91.03%), Query Frame = 0

Query: 1   MAVEASRVNTLSLTPISQADENYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDE 60
           MAV A+R+    LTP S+AD +YHR T+LKAFDDTKSGVKGLVDAGITEIPRIFY+PP+ 
Sbjct: 1   MAVAATRI----LTPASKADGSYHRPTELKAFDDTKSGVKGLVDAGITEIPRIFYQPPES 60

Query: 61  DHSGDVSGETQLHIPVIDLNHVGRNSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEE 120
           + SG VS ETQ+HIPVI+L+H+G+  LKRKYT+DRIR ASEK GFFQLINHGIPVSVLEE
Sbjct: 61  NDSGHVSDETQIHIPVINLDHIGKTPLKRKYTVDRIREASEKFGFFQLINHGIPVSVLEE 120

Query: 121 MKDAVRRFHEQDTELKKQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQ 180
           MKD VRRFHEQ+TELKKQYY+RN+TKPLIYTSNFDL+SA TTNWRD FRY S PNSH+P+
Sbjct: 121 MKDGVRRFHEQETELKKQYYSRNLTKPLIYTSNFDLYSAPTTNWRDAFRYISVPNSHNPE 180

Query: 181 DLPEICRDILVDYSKRVMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQ 240
           DLPEICRDILVDYSKRVMEIGKL+F+LLSEALGL+ NYLN +DC EGL+L+CHYYPPCPQ
Sbjct: 181 DLPEICRDILVDYSKRVMEIGKLVFELLSEALGLNPNYLNGVDCGEGLSLVCHYYPPCPQ 240

Query: 241 PNLTIGTSEHTDNTFITVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQASFCKI 300
           PNL IGTSEHTDN+FITVLLQDHIGGLQIRHENNWVD+PPVAGALVVNIGDLMQASF  I
Sbjct: 241 PNLAIGTSEHTDNSFITVLLQDHIGGLQIRHENNWVDIPPVAGALVVNIGDLMQASFSTI 297

Query: 301 S 302
           S
Sbjct: 301 S 297

BLAST of Spg031305 vs. NCBI nr
Match: XP_038877346.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like [Benincasa hispida])

HSP 1 Score: 518.8 bits (1335), Expect = 3.2e-143
Identity = 246/294 (83.67%), Postives = 271/294 (92.18%), Query Frame = 0

Query: 1   MAVEASRVNTLSLTPISQADENYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDE 60
           MAV ASRVN  +LTP+S+ADENYHR TDLKAFDDTK+GVKGLVDAGITEIPRIFY+PP+ 
Sbjct: 1   MAVAASRVNGPNLTPLSKADENYHRPTDLKAFDDTKTGVKGLVDAGITEIPRIFYRPPEN 60

Query: 61  DHSGDVSGETQLHIPVIDLNHVGRNSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEE 120
             S +VSGETQ+HIPVIDL+ + +NSLKRKYTIDR+R ASEKLGFFQLINHGIPV VLEE
Sbjct: 61  YDSDNVSGETQIHIPVIDLDQINKNSLKRKYTIDRVREASEKLGFFQLINHGIPVDVLEE 120

Query: 121 MKDAVRRFHEQDTELKKQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQ 180
           MKDAVRRFHEQ+ E +KQYYTR++TKPLIY SNFDL+SAATTNWRDTF Y SAPNSH+PQ
Sbjct: 121 MKDAVRRFHEQEAESRKQYYTRDLTKPLIYNSNFDLYSAATTNWRDTFGYISAPNSHNPQ 180

Query: 181 DLPEICRDILVDYSKRVMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQ 240
           DLPEICRD+LVDYSKRVMEIG LLF+LLSEALGL+ NYL +IDC+EGLAL+CHYYPPCPQ
Sbjct: 181 DLPEICRDVLVDYSKRVMEIGNLLFELLSEALGLNPNYLKDIDCNEGLALVCHYYPPCPQ 240

Query: 241 PNLTIGTSEHTDNTFITVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQ 295
           PNL IGTSEHTDN FITVLLQDHIGGLQIR+EN WVDVPPVAGALVVNIGDLMQ
Sbjct: 241 PNLAIGTSEHTDNDFITVLLQDHIGGLQIRYENKWVDVPPVAGALVVNIGDLMQ 294

BLAST of Spg031305 vs. NCBI nr
Match: XP_022942354.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 516.2 bits (1328), Expect = 2.1e-142
Identity = 240/294 (81.63%), Postives = 268/294 (91.16%), Query Frame = 0

Query: 1   MAVEASRVNTLSLTPISQADENYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDE 60
           MAV A+ VNTL+LTP+S+ADE+YHR T++KAFDDTKSGVKGLVDAGITEIPRIFY+PP+ 
Sbjct: 1   MAVAATEVNTLNLTPVSKADESYHRPTEIKAFDDTKSGVKGLVDAGITEIPRIFYQPPES 60

Query: 61  DHSGDVSGETQLHIPVIDLNHVGRNSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEE 120
             SG VS ETQ+HIPVIDL+H+G+ SLKR+YT+DRIR ASEK GFFQLINHGIPVSVLEE
Sbjct: 61  SDSGHVSDETQIHIPVIDLDHIGKTSLKRRYTVDRIREASEKFGFFQLINHGIPVSVLEE 120

Query: 121 MKDAVRRFHEQDTELKKQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQ 180
           MKD VRRFHEQDTELK QYY+RN+ +P IY SNFDL+S+ATTNWRDTFRY SAPNSH PQ
Sbjct: 121 MKDGVRRFHEQDTELKAQYYSRNIMRPFIYISNFDLYSSATTNWRDTFRYLSAPNSHHPQ 180

Query: 181 DLPEICRDILVDYSKRVMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQ 240
            LPEICRDIL DYSKRVMEIGKL+F+LLSEALGL  NYLN+IDCSEGLAL+CHYYPPCPQ
Sbjct: 181 KLPEICRDILEDYSKRVMEIGKLMFELLSEALGLPPNYLNDIDCSEGLALVCHYYPPCPQ 240

Query: 241 PNLTIGTSEHTDNTFITVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQ 295
           PN+ IGTSEHTDN FIT+LLQDHIGGLQ+RH N WVDVPPVAGALVVNIGDL+Q
Sbjct: 241 PNMAIGTSEHTDNDFITILLQDHIGGLQVRHGNKWVDVPPVAGALVVNIGDLLQ 294

BLAST of Spg031305 vs. NCBI nr
Match: XP_022942332.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 510.0 bits (1312), Expect = 1.5e-140
Identity = 238/294 (80.95%), Postives = 270/294 (91.84%), Query Frame = 0

Query: 1   MAVEASRVNTLSLTPISQADENYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDE 60
           MAV A+R+    LTP+S+AD +YHR T+LKAFDDTKSGVKGLVDAGITEIPRIFY+PP+ 
Sbjct: 1   MAVAATRI----LTPVSKADGSYHRPTELKAFDDTKSGVKGLVDAGITEIPRIFYQPPES 60

Query: 61  DHSGDVSGETQLHIPVIDLNHVGRNSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEE 120
           + SG VS ETQ+HIP+I+L+H+G+  LKRKYT+DRIR ASEK GFFQLINHGIPVSVLEE
Sbjct: 61  NDSGHVSDETQIHIPMINLDHIGKTPLKRKYTVDRIREASEKFGFFQLINHGIPVSVLEE 120

Query: 121 MKDAVRRFHEQDTELKKQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQ 180
           MKD VRRFHEQ+TELKKQYY+RN+TKPLIYTSNFDL+SA TTNWRD FRY S P+SH+P+
Sbjct: 121 MKDGVRRFHEQETELKKQYYSRNLTKPLIYTSNFDLYSAPTTNWRDAFRYISVPSSHNPE 180

Query: 181 DLPEICRDILVDYSKRVMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQ 240
           DLPEICRDIL+DYSKRVMEIGKL+F+LLSEALGL+ NYLN +DC EGL+L+CHYYPPCPQ
Sbjct: 181 DLPEICRDILMDYSKRVMEIGKLVFELLSEALGLNPNYLNGVDCGEGLSLVCHYYPPCPQ 240

Query: 241 PNLTIGTSEHTDNTFITVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQ 295
           PNL IGTSEHTDN+FITVLLQDHIGGLQIRHENNWVD+PPVAGALVVNIGDLMQ
Sbjct: 241 PNLAIGTSEHTDNSFITVLLQDHIGGLQIRHENNWVDIPPVAGALVVNIGDLMQ 290

BLAST of Spg031305 vs. NCBI nr
Match: XP_022942317.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 510.0 bits (1312), Expect = 1.5e-140
Identity = 238/294 (80.95%), Postives = 270/294 (91.84%), Query Frame = 0

Query: 1   MAVEASRVNTLSLTPISQADENYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDE 60
           MAV A+R+    LTP+S+AD +YHR T+LKAFDDTKSGVKGLVDAGITEIPRIFY+PP+ 
Sbjct: 1   MAVAATRI----LTPVSKADGSYHRPTELKAFDDTKSGVKGLVDAGITEIPRIFYQPPES 60

Query: 61  DHSGDVSGETQLHIPVIDLNHVGRNSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEE 120
           + SG VS ETQ+HIP+I+L+H+G+  LKRKYT+DRIR ASEK GFFQLINHGIPVSVLEE
Sbjct: 61  NDSGHVSDETQIHIPMINLDHIGKTPLKRKYTVDRIREASEKFGFFQLINHGIPVSVLEE 120

Query: 121 MKDAVRRFHEQDTELKKQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQ 180
           MKD VRRFHEQ+TELKKQYY+RN+TKPLIYTSNFDL+SA TTNWRD FRY S P+SH+P+
Sbjct: 121 MKDGVRRFHEQETELKKQYYSRNLTKPLIYTSNFDLYSAPTTNWRDAFRYISVPSSHNPE 180

Query: 181 DLPEICRDILVDYSKRVMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQ 240
           DLPEICRDIL+DYSKRVMEIGKL+F+LLSEALGL+ NYLN +DC EGL+L+CHYYPPCPQ
Sbjct: 181 DLPEICRDILMDYSKRVMEIGKLVFELLSEALGLNPNYLNGVDCGEGLSLVCHYYPPCPQ 240

Query: 241 PNLTIGTSEHTDNTFITVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQ 295
           PNL IGTSEHTDN+FITVLLQDHIGGLQIRHENNWVD+PPVAGALVVNIGDLMQ
Sbjct: 241 PNLAIGTSEHTDNSFITVLLQDHIGGLQIRHENNWVDIPPVAGALVVNIGDLMQ 290

BLAST of Spg031305 vs. ExPASy Swiss-Prot
Match: Q84MB3 (1-aminocyclopropane-1-carboxylate oxidase homolog 1 OS=Arabidopsis thaliana OX=3702 GN=At1g06620 PE=2 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.1e-93
Identity = 157/270 (58.15%), Postives = 209/270 (77.41%), Query Frame = 0

Query: 25  RSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDEDHSGDVSGETQLHIPVIDLNHVGR 84
           RST LKAFD+TK+GVKGL+DAGITEIP IF  PP    S      +   IP IDL   G 
Sbjct: 13  RSTLLKAFDETKTGVKGLIDAGITEIPSIFRAPPATLTSPKPPSSSDFSIPTIDLKGGGT 72

Query: 85  NSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEEMKDAVRRFHEQDTELKKQYYTRNV 144
           +S+ R+  +++I  A+EK GFFQ+INHGIP+ VLE+M D +R FHEQDTE+KK +Y+R+ 
Sbjct: 73  DSITRRSLVEKIGDAAEKWGFFQVINHGIPMDVLEKMIDGIREFHEQDTEVKKGFYSRDP 132

Query: 145 TKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQDLPEICRDILVDYSKRVMEIGKLL 204
              ++Y+SNFDLFS+   NWRDT    +AP+   P+DLP  C +++++YSK VM++GKLL
Sbjct: 133 ASKMVYSSNFDLFSSPAANWRDTLGCYTAPDPPRPEDLPATCGEMMIEYSKEVMKLGKLL 192

Query: 205 FQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQPNLTIGTSEHTDNTFITVLLQDHI 264
           F+LLSEALGL++N+L ++DC+  L LL HYYPPCPQP+LT+G ++H+DN+F+T+LLQDHI
Sbjct: 193 FELLSEALGLNTNHLKDMDCTNSLLLLGHYYPPCPQPDLTLGLTKHSDNSFLTILLQDHI 252

Query: 265 GGLQIRHENNWVDVPPVAGALVVNIGDLMQ 295
           GGLQ+ H+  WVDVPPV GALVVN+GDL+Q
Sbjct: 253 GGLQVLHDQYWVDVPPVPGALVVNVGDLLQ 282

BLAST of Spg031305 vs. ExPASy Swiss-Prot
Match: Q8H1S4 (1-aminocyclopropane-1-carboxylate oxidase homolog 3 OS=Arabidopsis thaliana OX=3702 GN=At1g06650 PE=2 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 2.5e-90
Identity = 155/281 (55.16%), Postives = 217/281 (77.22%), Query Frame = 0

Query: 20  DENYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDEDHSGDVSGETQLH---IPV 79
           D  + R+++LKAFD+TK+GVKGLVD+G++++PRIF+ P  +  +        LH   IP 
Sbjct: 7   DPLFDRASELKAFDETKTGVKGLVDSGVSQVPRIFHHPTVKLSTPKPLPSDLLHLKTIPT 66

Query: 80  IDLNHVGR---NSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEEMKDAVRRFHEQDT 139
           IDL   GR   +++KR   I+ I+ A+ K GFFQ+INHG+ + +LE+MK  VR FHEQ  
Sbjct: 67  IDLG--GRDFQDAIKRNNAIEEIKEAAAKWGFFQVINHGVSLELLEKMKKGVRDFHEQSQ 126

Query: 140 ELKKQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQDLPEICRDILVDY 199
           E++K++Y+R+ ++  +Y SNFDLFS+   NWRDTF  T AP++  PQDLPEICRDI+++Y
Sbjct: 127 EVRKEFYSRDFSRRFLYLSNFDLFSSPAANWRDTFSCTMAPDTPKPQDLPEICRDIMMEY 186

Query: 200 SKRVMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQPNLTIGTSEHTDN 259
           SK+VM +GK LF+LLSEALGL+ N+LN++DCS+GL +L HYYPPCP+P+LT+GTS+H+DN
Sbjct: 187 SKQVMNLGKFLFELLSEALGLEPNHLNDMDCSKGLLMLSHYYPPCPEPDLTLGTSQHSDN 246

Query: 260 TFITVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQ 295
           +F+TVLL D I GLQ+R E +W DVP V+GAL++NIGDL+Q
Sbjct: 247 SFLTVLLPDQIEGLQVRREGHWFDVPHVSGALIINIGDLLQ 285

BLAST of Spg031305 vs. ExPASy Swiss-Prot
Match: Q9LSW6 (1-aminocyclopropane-1-carboxylate oxidase homolog 10 OS=Arabidopsis thaliana OX=3702 GN=At5g43450 PE=2 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 8.3e-86
Identity = 151/282 (53.55%), Postives = 203/282 (71.99%), Query Frame = 0

Query: 16  ISQADENYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPP---DEDHSGDVSGETQL 75
           +++  E   R  DL  F  TK+GVKGLVDA ITE+P +F+ P      +   D+SG   L
Sbjct: 1   MTENSEKIDRLNDLTTFISTKTGVKGLVDAEITEVPSMFHVPSSILSNNRPSDISG-LNL 60

Query: 76  HIPVIDLNHVGRNSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEEMKDAVRRFHEQD 135
            +P+IDL    RN+  R   I +I+ A+E  GFFQ+INH +P++VLEE+K++VRRFHEQD
Sbjct: 61  TVPIIDLG--DRNTSSRNVVISKIKDAAENWGFFQVINHDVPLTVLEEIKESVRRFHEQD 120

Query: 136 TELKKQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQDLPEICRDILVD 195
             +K QY   +  K  +Y ++FDL+ ++  NWRD+F    AP+  +P+++P  CR  +++
Sbjct: 121 PVVKNQYLPTDNNKRFVYNNDFDLYHSSPLNWRDSFTCYIAPDPPNPEEIPLACRSAVIE 180

Query: 196 YSKRVMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQPNLTIGTSEHTD 255
           Y+K VME+G +LFQLLSEALGLDS  L  IDC +GL +LCHYYPPCPQP+LT+G S+HTD
Sbjct: 181 YTKHVMELGAVLFQLLSEALGLDSETLKRIDCLKGLFMLCHYYPPCPQPDLTLGISKHTD 240

Query: 256 NTFITVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQ 295
           N+F+T+LLQD IGGLQ+ HE+ WVDVPPV GALVVNIGD MQ
Sbjct: 241 NSFLTLLLQDQIGGLQVLHEDYWVDVPPVPGALVVNIGDFMQ 279

BLAST of Spg031305 vs. ExPASy Swiss-Prot
Match: Q9LSW7 (1-aminocyclopropane-1-carboxylate oxidase homolog 9 OS=Arabidopsis thaliana OX=3702 GN=At5g43440 PE=2 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 7.1e-85
Identity = 150/275 (54.55%), Postives = 200/275 (72.73%), Query Frame = 0

Query: 25  RSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDEDHS----GDVSGETQLHIPVIDLN 84
           R  +LKAF  TK+GVKGLVD  ITE+PRIF+ P     S     D+ G   L +P+IDL 
Sbjct: 10  RLNELKAFVSTKAGVKGLVDTKITEVPRIFHIPSSSTLSNNKPSDIFG-LNLTVPIIDLG 69

Query: 85  HVGRNSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEEMKDAVRRFHEQDTELKKQYY 144
             G  S  R   + +I+ A+E  GFFQ+INHGIP++VL+++K  VRRFHE+D E+KKQY+
Sbjct: 70  D-GNTSAARNVLVSKIKEAAENWGFFQVINHGIPLTVLKDIKQGVRRFHEEDPEVKKQYF 129

Query: 145 TRNVTKPLIYTSNFDLFSAATTNWRDTFR-YTSAPNSHDPQDLPEICRDILVDYSKRVME 204
             +      Y +NFD+  ++  NW+D+F  YT   +   P+++P  CRD++++YSK VME
Sbjct: 130 ATDFNTRFAYNTNFDIHYSSPMNWKDSFTCYTCPQDPLKPEEIPLACRDVVIEYSKHVME 189

Query: 205 IGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQPNLTIGTSEHTDNTFITVL 264
           +G LLFQLLSEALGLDS  L N+DC +GL +LCHYYPPCPQP+LT+G S+HTDN+FIT+L
Sbjct: 190 LGGLLFQLLSEALGLDSEILKNMDCLKGLLMLCHYYPPCPQPDLTLGISKHTDNSFITIL 249

Query: 265 LQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQ 295
           LQD IGGLQ+ H+++WVDV PV GALV++IGD MQ
Sbjct: 250 LQDQIGGLQVLHQDSWVDVTPVPGALVISIGDFMQ 282

BLAST of Spg031305 vs. ExPASy Swiss-Prot
Match: Q9C5K7 (1-aminocyclopropane-1-carboxylate oxidase homolog 2 OS=Arabidopsis thaliana OX=3702 GN=At1g06640 PE=2 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.1e-84
Identity = 150/279 (53.76%), Postives = 209/279 (74.91%), Query Frame = 0

Query: 22  NYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDEDHSGDVSGETQLH---IPVID 81
           ++ R+++LKAFD+TK+GVKGLVD+GI++IPRIF+    E  +        LH   IP ID
Sbjct: 9   SFDRASELKAFDETKTGVKGLVDSGISKIPRIFHHSSVELANPKPLPSDLLHLKTIPTID 68

Query: 82  LNHVGR---NSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEEMKDAVRRFHEQDTEL 141
           L   GR   +++K K  I+ I+ A+ K GFFQ+INHG+ + +LE+MKD VR FHEQ  E+
Sbjct: 69  LG--GRDFQDAIKHKNAIEGIKEAAAKWGFFQVINHGVSLELLEKMKDGVRDFHEQPPEV 128

Query: 142 KKQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQDLPEICRDILVDYSK 201
           +K  Y+R+  +  IY SNFDL++AA  NWRDTF    AP+  +PQDLPEICRD++++YSK
Sbjct: 129 RKDLYSRDFGRKFIYLSNFDLYTAAAANWRDTFYCYMAPDPPEPQDLPEICRDVMMEYSK 188

Query: 202 RVMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQPNLTIGTSEHTDNTF 261
           +VM +G+ LF+LLSEALGL+ N+L +++C +GL +LCHY+PPCP+P+LT GTS+H+D +F
Sbjct: 189 QVMILGEFLFELLSEALGLNPNHLKDMECLKGLRMLCHYFPPCPEPDLTFGTSKHSDGSF 248

Query: 262 ITVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQ 295
           +TVLL D+I GLQ+  E  W DVP V GAL++NIGDL+Q
Sbjct: 249 LTVLLPDNIEGLQVCREGYWFDVPHVPGALIINIGDLLQ 285

BLAST of Spg031305 vs. ExPASy TrEMBL
Match: A0A6J1FR24 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111447413 PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 1.0e-142
Identity = 240/294 (81.63%), Postives = 268/294 (91.16%), Query Frame = 0

Query: 1   MAVEASRVNTLSLTPISQADENYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDE 60
           MAV A+ VNTL+LTP+S+ADE+YHR T++KAFDDTKSGVKGLVDAGITEIPRIFY+PP+ 
Sbjct: 1   MAVAATEVNTLNLTPVSKADESYHRPTEIKAFDDTKSGVKGLVDAGITEIPRIFYQPPES 60

Query: 61  DHSGDVSGETQLHIPVIDLNHVGRNSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEE 120
             SG VS ETQ+HIPVIDL+H+G+ SLKR+YT+DRIR ASEK GFFQLINHGIPVSVLEE
Sbjct: 61  SDSGHVSDETQIHIPVIDLDHIGKTSLKRRYTVDRIREASEKFGFFQLINHGIPVSVLEE 120

Query: 121 MKDAVRRFHEQDTELKKQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQ 180
           MKD VRRFHEQDTELK QYY+RN+ +P IY SNFDL+S+ATTNWRDTFRY SAPNSH PQ
Sbjct: 121 MKDGVRRFHEQDTELKAQYYSRNIMRPFIYISNFDLYSSATTNWRDTFRYLSAPNSHHPQ 180

Query: 181 DLPEICRDILVDYSKRVMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQ 240
            LPEICRDIL DYSKRVMEIGKL+F+LLSEALGL  NYLN+IDCSEGLAL+CHYYPPCPQ
Sbjct: 181 KLPEICRDILEDYSKRVMEIGKLMFELLSEALGLPPNYLNDIDCSEGLALVCHYYPPCPQ 240

Query: 241 PNLTIGTSEHTDNTFITVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQ 295
           PN+ IGTSEHTDN FIT+LLQDHIGGLQ+RH N WVDVPPVAGALVVNIGDL+Q
Sbjct: 241 PNMAIGTSEHTDNDFITILLQDHIGGLQVRHGNKWVDVPPVAGALVVNIGDLLQ 294

BLAST of Spg031305 vs. ExPASy TrEMBL
Match: A0A6J1FPY8 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111447392 PE=3 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 7.2e-141
Identity = 238/294 (80.95%), Postives = 270/294 (91.84%), Query Frame = 0

Query: 1   MAVEASRVNTLSLTPISQADENYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDE 60
           MAV A+R+    LTP+S+AD +YHR T+LKAFDDTKSGVKGLVDAGITEIPRIFY+PP+ 
Sbjct: 1   MAVAATRI----LTPVSKADGSYHRPTELKAFDDTKSGVKGLVDAGITEIPRIFYQPPES 60

Query: 61  DHSGDVSGETQLHIPVIDLNHVGRNSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEE 120
           + SG VS ETQ+HIP+I+L+H+G+  LKRKYT+DRIR ASEK GFFQLINHGIPVSVLEE
Sbjct: 61  NDSGHVSDETQIHIPMINLDHIGKTPLKRKYTVDRIREASEKFGFFQLINHGIPVSVLEE 120

Query: 121 MKDAVRRFHEQDTELKKQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQ 180
           MKD VRRFHEQ+TELKKQYY+RN+TKPLIYTSNFDL+SA TTNWRD FRY S P+SH+P+
Sbjct: 121 MKDGVRRFHEQETELKKQYYSRNLTKPLIYTSNFDLYSAPTTNWRDAFRYISVPSSHNPE 180

Query: 181 DLPEICRDILVDYSKRVMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQ 240
           DLPEICRDIL+DYSKRVMEIGKL+F+LLSEALGL+ NYLN +DC EGL+L+CHYYPPCPQ
Sbjct: 181 DLPEICRDILMDYSKRVMEIGKLVFELLSEALGLNPNYLNGVDCGEGLSLVCHYYPPCPQ 240

Query: 241 PNLTIGTSEHTDNTFITVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQ 295
           PNL IGTSEHTDN+FITVLLQDHIGGLQIRHENNWVD+PPVAGALVVNIGDLMQ
Sbjct: 241 PNLAIGTSEHTDNSFITVLLQDHIGGLQIRHENNWVDIPPVAGALVVNIGDLMQ 290

BLAST of Spg031305 vs. ExPASy TrEMBL
Match: A0A6J1FPX0 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111447392 PE=3 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 7.2e-141
Identity = 238/294 (80.95%), Postives = 270/294 (91.84%), Query Frame = 0

Query: 1   MAVEASRVNTLSLTPISQADENYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDE 60
           MAV A+R+    LTP+S+AD +YHR T+LKAFDDTKSGVKGLVDAGITEIPRIFY+PP+ 
Sbjct: 1   MAVAATRI----LTPVSKADGSYHRPTELKAFDDTKSGVKGLVDAGITEIPRIFYQPPES 60

Query: 61  DHSGDVSGETQLHIPVIDLNHVGRNSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEE 120
           + SG VS ETQ+HIP+I+L+H+G+  LKRKYT+DRIR ASEK GFFQLINHGIPVSVLEE
Sbjct: 61  NDSGHVSDETQIHIPMINLDHIGKTPLKRKYTVDRIREASEKFGFFQLINHGIPVSVLEE 120

Query: 121 MKDAVRRFHEQDTELKKQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQ 180
           MKD VRRFHEQ+TELKKQYY+RN+TKPLIYTSNFDL+SA TTNWRD FRY S P+SH+P+
Sbjct: 121 MKDGVRRFHEQETELKKQYYSRNLTKPLIYTSNFDLYSAPTTNWRDAFRYISVPSSHNPE 180

Query: 181 DLPEICRDILVDYSKRVMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQ 240
           DLPEICRDIL+DYSKRVMEIGKL+F+LLSEALGL+ NYLN +DC EGL+L+CHYYPPCPQ
Sbjct: 181 DLPEICRDILMDYSKRVMEIGKLVFELLSEALGLNPNYLNGVDCGEGLSLVCHYYPPCPQ 240

Query: 241 PNLTIGTSEHTDNTFITVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQ 295
           PNL IGTSEHTDN+FITVLLQDHIGGLQIRHENNWVD+PPVAGALVVNIGDLMQ
Sbjct: 241 PNLAIGTSEHTDNSFITVLLQDHIGGLQIRHENNWVDIPPVAGALVVNIGDLMQ 290

BLAST of Spg031305 vs. ExPASy TrEMBL
Match: A0A6J1FNM0 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447413 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.2e-140
Identity = 240/301 (79.73%), Postives = 268/301 (89.04%), Query Frame = 0

Query: 1   MAVEASRVNTLSLTPISQADENYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDE 60
           MAV A+ VNTL+LTP+S+ADE+YHR T++KAFDDTKSGVKGLVDAGITEIPRIFY+PP+ 
Sbjct: 1   MAVAATEVNTLNLTPVSKADESYHRPTEIKAFDDTKSGVKGLVDAGITEIPRIFYQPPES 60

Query: 61  DHSGDVSGETQLHIPVIDLNHVGRNSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEE 120
             SG VS ETQ+HIPVIDL+H+G+ SLKR+YT+DRIR ASEK GFFQLINHGIPVSVLEE
Sbjct: 61  SDSGHVSDETQIHIPVIDLDHIGKTSLKRRYTVDRIREASEKFGFFQLINHGIPVSVLEE 120

Query: 121 MKDAVRRFHEQDTELKKQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQ 180
           MKD VRRFHEQDTELK QYY+RN+ +P IY SNFDL+S+ATTNWRDTFRY SAPNSH PQ
Sbjct: 121 MKDGVRRFHEQDTELKAQYYSRNIMRPFIYISNFDLYSSATTNWRDTFRYLSAPNSHHPQ 180

Query: 181 DLPEI-------CRDILVDYSKRVMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCH 240
            LPEI       CRDIL DYSKRVMEIGKL+F+LLSEALGL  NYLN+IDCSEGLAL+CH
Sbjct: 181 KLPEICRDSSVLCRDILEDYSKRVMEIGKLMFELLSEALGLPPNYLNDIDCSEGLALVCH 240

Query: 241 YYPPCPQPNLTIGTSEHTDNTFITVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLM 295
           YYPPCPQPN+ IGTSEHTDN FIT+LLQDHIGGLQ+RH N WVDVPPVAGALVVNIGDL+
Sbjct: 241 YYPPCPQPNMAIGTSEHTDNDFITILLQDHIGGLQVRHGNKWVDVPPVAGALVVNIGDLL 300

BLAST of Spg031305 vs. ExPASy TrEMBL
Match: A0A6J1FQZ4 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111447392 PE=3 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 8.8e-139
Identity = 238/301 (79.07%), Postives = 270/301 (89.70%), Query Frame = 0

Query: 1   MAVEASRVNTLSLTPISQADENYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDE 60
           MAV A+R+    LTP+S+AD +YHR T+LKAFDDTKSGVKGLVDAGITEIPRIFY+PP+ 
Sbjct: 1   MAVAATRI----LTPVSKADGSYHRPTELKAFDDTKSGVKGLVDAGITEIPRIFYQPPES 60

Query: 61  DHSGDVSGETQLHIPVIDLNHVGRNSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEE 120
           + SG VS ETQ+HIP+I+L+H+G+  LKRKYT+DRIR ASEK GFFQLINHGIPVSVLEE
Sbjct: 61  NDSGHVSDETQIHIPMINLDHIGKTPLKRKYTVDRIREASEKFGFFQLINHGIPVSVLEE 120

Query: 121 MKDAVRRFHEQDTELKKQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQ 180
           MKD VRRFHEQ+TELKKQYY+RN+TKPLIYTSNFDL+SA TTNWRD FRY S P+SH+P+
Sbjct: 121 MKDGVRRFHEQETELKKQYYSRNLTKPLIYTSNFDLYSAPTTNWRDAFRYISVPSSHNPE 180

Query: 181 DLPEI-------CRDILVDYSKRVMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCH 240
           DLPEI       CRDIL+DYSKRVMEIGKL+F+LLSEALGL+ NYLN +DC EGL+L+CH
Sbjct: 181 DLPEICRGSSVLCRDILMDYSKRVMEIGKLVFELLSEALGLNPNYLNGVDCGEGLSLVCH 240

Query: 241 YYPPCPQPNLTIGTSEHTDNTFITVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLM 295
           YYPPCPQPNL IGTSEHTDN+FITVLLQDHIGGLQIRHENNWVD+PPVAGALVVNIGDLM
Sbjct: 241 YYPPCPQPNLAIGTSEHTDNSFITVLLQDHIGGLQIRHENNWVDIPPVAGALVVNIGDLM 297

BLAST of Spg031305 vs. TAIR 10
Match: AT1G06620.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 344.7 bits (883), Expect = 7.7e-95
Identity = 157/270 (58.15%), Postives = 209/270 (77.41%), Query Frame = 0

Query: 25  RSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDEDHSGDVSGETQLHIPVIDLNHVGR 84
           RST LKAFD+TK+GVKGL+DAGITEIP IF  PP    S      +   IP IDL   G 
Sbjct: 13  RSTLLKAFDETKTGVKGLIDAGITEIPSIFRAPPATLTSPKPPSSSDFSIPTIDLKGGGT 72

Query: 85  NSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEEMKDAVRRFHEQDTELKKQYYTRNV 144
           +S+ R+  +++I  A+EK GFFQ+INHGIP+ VLE+M D +R FHEQDTE+KK +Y+R+ 
Sbjct: 73  DSITRRSLVEKIGDAAEKWGFFQVINHGIPMDVLEKMIDGIREFHEQDTEVKKGFYSRDP 132

Query: 145 TKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQDLPEICRDILVDYSKRVMEIGKLL 204
              ++Y+SNFDLFS+   NWRDT    +AP+   P+DLP  C +++++YSK VM++GKLL
Sbjct: 133 ASKMVYSSNFDLFSSPAANWRDTLGCYTAPDPPRPEDLPATCGEMMIEYSKEVMKLGKLL 192

Query: 205 FQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQPNLTIGTSEHTDNTFITVLLQDHI 264
           F+LLSEALGL++N+L ++DC+  L LL HYYPPCPQP+LT+G ++H+DN+F+T+LLQDHI
Sbjct: 193 FELLSEALGLNTNHLKDMDCTNSLLLLGHYYPPCPQPDLTLGLTKHSDNSFLTILLQDHI 252

Query: 265 GGLQIRHENNWVDVPPVAGALVVNIGDLMQ 295
           GGLQ+ H+  WVDVPPV GALVVN+GDL+Q
Sbjct: 253 GGLQVLHDQYWVDVPPVPGALVVNVGDLLQ 282

BLAST of Spg031305 vs. TAIR 10
Match: AT1G06650.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.1e-92
Identity = 158/300 (52.67%), Postives = 223/300 (74.33%), Query Frame = 0

Query: 20  DENYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDEDHSGDVSGETQLH---IPV 79
           D  + R+++LKAFD+TK+GVKGLVD+G++++PRIF+ P  +  +        LH   IP 
Sbjct: 7   DPLFDRASELKAFDETKTGVKGLVDSGVSQVPRIFHHPTVKLSTPKPLPSDLLHLKTIPT 66

Query: 80  IDLNHVGR---NSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEEMKDAVRRFHEQDT 139
           IDL   GR   +++KR   I+ I+ A+ K GFFQ+INHG+ + +LE+MK  VR FHEQ  
Sbjct: 67  IDLG--GRDFQDAIKRNNAIEEIKEAAAKWGFFQVINHGVSLELLEKMKKGVRDFHEQSQ 126

Query: 140 ELKKQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQDLPEICRDILVDY 199
           E++K++Y+R+ ++  +Y SNFDLFS+   NWRDTF  T AP++  PQDLPEICRDI+++Y
Sbjct: 127 EVRKEFYSRDFSRRFLYLSNFDLFSSPAANWRDTFSCTMAPDTPKPQDLPEICRDIMMEY 186

Query: 200 SKRVMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQPNLTIGTSEHTDN 259
           SK+VM +GK LF+LLSEALGL+ N+LN++DCS+GL +L HYYPPCP+P+LT+GTS+H+DN
Sbjct: 187 SKQVMNLGKFLFELLSEALGLEPNHLNDMDCSKGLLMLSHYYPPCPEPDLTLGTSQHSDN 246

Query: 260 TFITVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQASFCKISFSANDEYPLIRL 314
           +F+TVLL D I GLQ+R E +W DVP V+GAL++NIGDL+Q     I   +N    + R+
Sbjct: 247 SFLTVLLPDQIEGLQVRREGHWFDVPHVSGALIINIGDLLQFGAQSIGEQSNKSSSVCRM 304

BLAST of Spg031305 vs. TAIR 10
Match: AT1G06650.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 333.6 bits (854), Expect = 1.8e-91
Identity = 155/281 (55.16%), Postives = 217/281 (77.22%), Query Frame = 0

Query: 20  DENYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDEDHSGDVSGETQLH---IPV 79
           D  + R+++LKAFD+TK+GVKGLVD+G++++PRIF+ P  +  +        LH   IP 
Sbjct: 7   DPLFDRASELKAFDETKTGVKGLVDSGVSQVPRIFHHPTVKLSTPKPLPSDLLHLKTIPT 66

Query: 80  IDLNHVGR---NSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEEMKDAVRRFHEQDT 139
           IDL   GR   +++KR   I+ I+ A+ K GFFQ+INHG+ + +LE+MK  VR FHEQ  
Sbjct: 67  IDLG--GRDFQDAIKRNNAIEEIKEAAAKWGFFQVINHGVSLELLEKMKKGVRDFHEQSQ 126

Query: 140 ELKKQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQDLPEICRDILVDY 199
           E++K++Y+R+ ++  +Y SNFDLFS+   NWRDTF  T AP++  PQDLPEICRDI+++Y
Sbjct: 127 EVRKEFYSRDFSRRFLYLSNFDLFSSPAANWRDTFSCTMAPDTPKPQDLPEICRDIMMEY 186

Query: 200 SKRVMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQPNLTIGTSEHTDN 259
           SK+VM +GK LF+LLSEALGL+ N+LN++DCS+GL +L HYYPPCP+P+LT+GTS+H+DN
Sbjct: 187 SKQVMNLGKFLFELLSEALGLEPNHLNDMDCSKGLLMLSHYYPPCPEPDLTLGTSQHSDN 246

Query: 260 TFITVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQ 295
           +F+TVLL D I GLQ+R E +W DVP V+GAL++NIGDL+Q
Sbjct: 247 SFLTVLLPDQIEGLQVRREGHWFDVPHVSGALIINIGDLLQ 285

BLAST of Spg031305 vs. TAIR 10
Match: AT2G30840.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 323.2 bits (827), Expect = 2.4e-88
Identity = 149/278 (53.60%), Postives = 210/278 (75.54%), Query Frame = 0

Query: 20  DENYHRSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPP-DEDHSGDVSGETQLHIPVID 79
           +  Y R++++KAFD+ K GVKGL+DAG+T+IPRIF+ P  +   S  +   T + IP ID
Sbjct: 2   EATYDRASEVKAFDELKIGVKGLLDAGVTQIPRIFHHPHLNLTDSNLLLSSTTMVIPTID 61

Query: 80  LNH--VGRNSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEEMKDAVRRFHEQDTELK 139
           L        ++ R+  I  IR A E+ GFFQ+INHGI   V+E+MKD +R FHEQD++++
Sbjct: 62  LKGGVFDEYTVTRESVIAMIRDAVERFGFFQVINHGISNDVMEKMKDGIRGFHEQDSDVR 121

Query: 140 KQYYTRNVTKPLIYTSNFDLFSAATTNWRDTFRYTSAPNSHDPQDLPEICRDILVDYSKR 199
           K++YTR+VTK + Y SNFDL+S+ + NWRDT     AP+  + +DLP+IC +I+++Y+KR
Sbjct: 122 KKFYTRDVTKTVKYNSNFDLYSSPSANWRDTLSCFMAPDVPETEDLPDICGEIMLEYAKR 181

Query: 200 VMEIGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQPNLTIGTSEHTDNTFI 259
           VM++G+L+F+LLSEALGL+ N+L  +DC++GL +L HYYPPCP+P LT GTS H+D +F+
Sbjct: 182 VMKLGELIFELLSEALGLNPNHLKEMDCTKGLLMLSHYYPPCPEPGLTFGTSPHSDRSFL 241

Query: 260 TVLLQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQ 295
           T+LLQDHIGGLQ+R    WVDVPPV GAL+VN+GDL+Q
Sbjct: 242 TILLQDHIGGLQVRQNGYWVDVPPVPGALLVNLGDLLQ 279

BLAST of Spg031305 vs. TAIR 10
Match: AT5G43440.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 320.9 bits (821), Expect = 1.2e-87
Identity = 154/283 (54.42%), Postives = 204/283 (72.08%), Query Frame = 0

Query: 25  RSTDLKAFDDTKSGVKGLVDAGITEIPRIFYKPPDEDHS----GDVSGETQLHIPVIDLN 84
           R  +LKAF  TK+GVKGLVD  ITE+PRIF+ P     S     D+ G   L +P+IDL 
Sbjct: 10  RLNELKAFVSTKAGVKGLVDTKITEVPRIFHIPSSSTLSNNKPSDIFG-LNLTVPIIDLG 69

Query: 85  HVGRNSLKRKYTIDRIRGASEKLGFFQLINHGIPVSVLEEMKDAVRRFHEQDTELKKQYY 144
             G  S  R   + +I+ A+E  GFFQ+INHGIP++VL+++K  VRRFHE+D E+KKQY+
Sbjct: 70  D-GNTSAARNVLVSKIKEAAENWGFFQVINHGIPLTVLKDIKQGVRRFHEEDPEVKKQYF 129

Query: 145 TRNVTKPLIYTSNFDLFSAATTNWRDTFR-YTSAPNSHDPQDLPEICRDILVDYSKRVME 204
             +      Y +NFD+  ++  NW+D+F  YT   +   P+++P  CRD++++YSK VME
Sbjct: 130 ATDFNTRFAYNTNFDIHYSSPMNWKDSFTCYTCPQDPLKPEEIPLACRDVVIEYSKHVME 189

Query: 205 IGKLLFQLLSEALGLDSNYLNNIDCSEGLALLCHYYPPCPQPNLTIGTSEHTDNTFITVL 264
           +G LLFQLLSEALGLDS  L N+DC +GL +LCHYYPPCPQP+LT+G S+HTDN+FIT+L
Sbjct: 190 LGGLLFQLLSEALGLDSEILKNMDCLKGLLMLCHYYPPCPQPDLTLGISKHTDNSFITIL 249

Query: 265 LQDHIGGLQIRHENNWVDVPPVAGALVVNIGDLMQASFCKISF 303
           LQD IGGLQ+ H+++WVDV PV GALV++IGD MQAS    SF
Sbjct: 250 LQDQIGGLQVLHQDSWVDVTPVPGALVISIGDFMQASSIDASF 290

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6597376.18.4e-14481.731-aminocyclopropane-1-carboxylate oxidase-like 1, partial [Cucurbita argyrosperm... [more]
XP_038877346.13.2e-14383.671-aminocyclopropane-1-carboxylate oxidase homolog 1-like [Benincasa hispida][more]
XP_022942354.12.1e-14281.631-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 [Cucurbita m... [more]
XP_022942332.11.5e-14080.951-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X4 [Cucurbita m... [more]
XP_022942317.11.5e-14080.951-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 [Cucurbita m... [more]
Match NameE-valueIdentityDescription
Q84MB31.1e-9358.151-aminocyclopropane-1-carboxylate oxidase homolog 1 OS=Arabidopsis thaliana OX=3... [more]
Q8H1S42.5e-9055.161-aminocyclopropane-1-carboxylate oxidase homolog 3 OS=Arabidopsis thaliana OX=3... [more]
Q9LSW68.3e-8653.551-aminocyclopropane-1-carboxylate oxidase homolog 10 OS=Arabidopsis thaliana OX=... [more]
Q9LSW77.1e-8554.551-aminocyclopropane-1-carboxylate oxidase homolog 9 OS=Arabidopsis thaliana OX=3... [more]
Q9C5K72.1e-8453.761-aminocyclopropane-1-carboxylate oxidase homolog 2 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1FR241.0e-14281.631-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 OS=Cucurbita... [more]
A0A6J1FPY87.2e-14180.951-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X4 OS=Cucurbita... [more]
A0A6J1FPX07.2e-14180.951-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 OS=Cucurbita... [more]
A0A6J1FNM01.2e-14079.731-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X1 OS=Cucurbita... [more]
A0A6J1FQZ48.8e-13979.071-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X3 OS=Cucurbita... [more]
Match NameE-valueIdentityDescription
AT1G06620.17.7e-9558.152-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G06650.12.1e-9252.672-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G06650.21.8e-9155.162-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G30840.12.4e-8853.602-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G43440.21.2e-8754.422-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 118..138
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 15..295
NoneNo IPR availablePANTHERPTHR10209:SF653CME8 PROTEINcoord: 15..295
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 36..295
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 29..308
e-value: 4.0E-81
score: 275.1
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 74..180
e-value: 2.9E-17
score: 63.5
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 230..295
e-value: 5.2E-17
score: 62.2
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 226..319
score: 10.606911

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg031305.1Spg031305.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity