Spg031277 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg031277 vs. NCBI nr
Match: XP_022154796.1 (MATH domain-containing protein At5g43560-like isoform X1 [Momordica charantia] >XP_022154800.1 MATH domain-containing protein At5g43560-like isoform X1 [Momordica charantia])

HSP 1 Score: 556.2 bits (1432), Expect = 2.6e-154
Identity = 312/456 (68.42%), Postives = 346/456 (75.88%), Query Frame = 0

Query: 49  MDSIVGDEAEQIRSME-SIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTDDDDDD 108
           M S+V +EAE +RS++ SI N   S  GEALAEW+  EQVENG PST+  Y DTDDDDDD
Sbjct: 1   MASVVSEEAEGMRSVDSSIANGHHSQPGEALAEWRSSEQVENGTPSTSPPYWDTDDDDDD 60

Query: 109 DDDTGVLVILTSIRPNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGC 168
                         P PSELYGK+TWKIEKFSQLN+RE+RSN FEVG YKWYIL+YP+GC
Sbjct: 61  ------------AGPKPSELYGKHTWKIEKFSQLNKRELRSNAFEVGGYKWYILIYPQGC 120

Query: 169 DDARGRNHLSLFLSVANHGNLLAGWSHFAQFTMSVMNKDPKKSKYS-------------G 228
           D     NHLSLFL VANH  LL GWSHFAQFT++V+NKDPKKSKYS             G
Sbjct: 121 DVC---NHLSLFLCVANHDKLLPGWSHFAQFTIAVVNKDPKKSKYSDTLHRFWKKEHDWG 180

Query: 229 W----------DG-------------------ADRPFRCLDRQYRRKLIRVYLTNVEQIS 288
           W          DG                   ADRPFRCLD QYRR+L+RVYLTNVEQI 
Sbjct: 181 WKKFMELSKVLDGFIDADTLIIKAQVQVIRERADRPFRCLDCQYRRELVRVYLTNVEQIC 240

Query: 289 QRFVQERRRKLRKLIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVVKHFFIERE 348
           +RFV+ERR KL KLIEDKA+WSSFR FWLG DQNAR+ MSREKT+ +LKVVVKHFFIE+E
Sbjct: 241 RRFVEERRGKLGKLIEDKARWSSFRAFWLGIDQNARRRMSREKTDTILKVVVKHFFIEKE 300

Query: 349 VTSTLVMDSLYSGLKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVDDVLLLLER 408
           VTSTLVMDSLYSGLKALEGHTKCKKGKAKLLD+EEMPAPIVHIEKDTFVLVDDVLLLLER
Sbjct: 301 VTSTLVMDSLYSGLKALEGHTKCKKGKAKLLDAEEMPAPIVHIEKDTFVLVDDVLLLLER 360

Query: 409 TAMESLPPKDE-DPQNHTKAGSS-KDFDTDSTERDERRFTESGRRNLEIFVLDHIFSSKI 460
            +ME LPPKDE  PQN TK GSS +DF+ DS ERDERR TE GRR +EIFVL HIFSSK+
Sbjct: 361 ASMEPLPPKDEKGPQNRTKDGSSGEDFNKDSIERDERRLTELGRRTVEIFVLAHIFSSKV 420

BLAST of Spg031277 vs. NCBI nr
Match: XP_038877173.1 (TNF receptor-associated factor homolog 1a isoform X1 [Benincasa hispida])

HSP 1 Score: 553.9 bits (1426), Expect = 1.3e-153
Identity = 310/455 (68.13%), Postives = 344/455 (75.60%), Query Frame = 0

Query: 49  MDSIVGDEAEQIRSMESIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTDDDDDDD 108
           M  +V +EA  +RS+ES  N   S SGEALAEW+  EQVENG PST+  Y DTDDDDDD 
Sbjct: 1   MAGVVSEEAVGMRSVESFANGHHSQSGEALAEWRSSEQVENGTPSTSPPYWDTDDDDDD- 60

Query: 109 DDTGVLVILTSIRPNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGCD 168
                        P PS+LYGK+TWKIEKFSQLN+RE+RSN FEVG YKWYIL+YP+GCD
Sbjct: 61  -----------AGPKPSDLYGKHTWKIEKFSQLNKRELRSNAFEVGGYKWYILIYPQGCD 120

Query: 169 DARGRNHLSLFLSVANHGNLLAGWSHFAQFTMSVMNKDPKKSKYS-------------GW 228
                NHLSLFL VANH  LL GWSHFAQFT++V+NKDPKKSKYS             GW
Sbjct: 121 VC---NHLSLFLCVANHDKLLPGWSHFAQFTIAVVNKDPKKSKYSDTLHRFWKKEHDWGW 180

Query: 229 ----------DG-------------------ADRPFRCLDRQYRRKLIRVYLTNVEQISQ 288
                     DG                   ADRPFRCLD QYRR+L+RVYLTNVEQI +
Sbjct: 181 KKFMELSKVLDGFIDADTLIIKAQVQVIRERADRPFRCLDCQYRRELVRVYLTNVEQICR 240

Query: 289 RFVQERRRKLRKLIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVVKHFFIEREV 348
           RFV+ERR KL KLIEDKA+WSSFR FWLG DQNAR+ MSREKT+A+LKVVVKHFFIE+EV
Sbjct: 241 RFVEERRSKLGKLIEDKARWSSFRAFWLGIDQNARRRMSREKTDAILKVVVKHFFIEKEV 300

Query: 349 TSTLVMDSLYSGLKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVDDVLLLLERT 408
           TSTLVMDSLYSGLKALEGHTK KKGKAKLLD+EEM APIVHIEKDTFVLVDDVLLLLER 
Sbjct: 301 TSTLVMDSLYSGLKALEGHTKSKKGKAKLLDAEEMTAPIVHIEKDTFVLVDDVLLLLERA 360

Query: 409 AMESLPPKDE-DPQNHTKAGSS-KDFDTDSTERDERRFTESGRRNLEIFVLDHIFSSKIE 460
           A+E LPPKDE  PQN TK GSS +DF+ DS ERDERR TE GRR +EIFVL HIFSSK+E
Sbjct: 361 AVEPLPPKDEKGPQNRTKDGSSGEDFNKDSIERDERRLTELGRRTVEIFVLAHIFSSKVE 420

BLAST of Spg031277 vs. NCBI nr
Match: XP_022154806.1 (MATH domain-containing protein At5g43560-like isoform X2 [Momordica charantia])

HSP 1 Score: 550.1 bits (1416), Expect = 1.9e-152
Identity = 311/456 (68.20%), Postives = 345/456 (75.66%), Query Frame = 0

Query: 49  MDSIVGDEAEQIRSME-SIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTDDDDDD 108
           M S+V +EAE +RS++ SI N   S  GEALAEW+  EQVENG PST+  Y DTDDDDDD
Sbjct: 1   MASVVSEEAEGMRSVDSSIANGHHSQPGEALAEWRSSEQVENGTPSTSPPYWDTDDDDDD 60

Query: 109 DDDTGVLVILTSIRPNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGC 168
                         P PSELYGK+TWKIEKFSQLN+RE+RSN FEVG YKWYIL+YP+GC
Sbjct: 61  ------------AGPKPSELYGKHTWKIEKFSQLNKRELRSNAFEVGGYKWYILIYPQGC 120

Query: 169 DDARGRNHLSLFLSVANHGNLLAGWSHFAQFTMSVMNKDPKKSKYS-------------G 228
           D     NHLSLFL VANH  LL GWSHFAQFT++V+NKDPKKSKYS             G
Sbjct: 121 DVC---NHLSLFLCVANHDKLLPGWSHFAQFTIAVVNKDPKKSKYSDTLHRFWKKEHDWG 180

Query: 229 W----------DG-------------------ADRPFRCLDRQYRRKLIRVYLTNVEQIS 288
           W          DG                   ADRPFRCLD QYRR+L+RVYLTNVEQI 
Sbjct: 181 WKKFMELSKVLDGFIDADTLIIKAQVQVIRERADRPFRCLDCQYRRELVRVYLTNVEQIC 240

Query: 289 QRFVQERRRKLRKLIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVVKHFFIERE 348
           +RFV+ERR KL KLIEDKA+WSSFR FWLG DQNAR+ MSREKT+ +LKVVVKHFFIE+E
Sbjct: 241 RRFVEERRGKLGKLIEDKARWSSFRAFWLGIDQNARRRMSREKTDTILKVVVKHFFIEKE 300

Query: 349 VTSTLVMDSLYSGLKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVDDVLLLLER 408
           VTSTLVMDSLYSGLKALEGHTKCKKGKAKLLD+EEMPAPIVHIEKDTFVLVDDVLLLLER
Sbjct: 301 VTSTLVMDSLYSGLKALEGHTKCKKGKAKLLDAEEMPAPIVHIEKDTFVLVDDVLLLLER 360

Query: 409 TAMESLPPKDE-DPQNHTKAGSS-KDFDTDSTERDERRFTESGRRNLEIFVLDHIFSSKI 460
            +ME LPPKDE  PQN TK GSS +DF+ DS ERDERR TE GRR +EIFVL HIF SK+
Sbjct: 361 ASMEPLPPKDEKGPQNRTKDGSSGEDFNKDSIERDERRLTELGRRTVEIFVLAHIF-SKV 420

BLAST of Spg031277 vs. NCBI nr
Match: KAG7018773.1 (MATH domain-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 550.1 bits (1416), Expect = 1.9e-152
Identity = 307/461 (66.59%), Postives = 347/461 (75.27%), Query Frame = 0

Query: 43  ISSERKMDSIVGDEAEQIRSMESIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTD 102
           +SSERKM   V +EA  +RS+ES  N   S SGEALAEW+  EQVENG PST+  Y DTD
Sbjct: 72  LSSERKMAGAVSEEALGMRSVESFANGHHSQSGEALAEWRSSEQVENGTPSTSPPYWDTD 131

Query: 103 DDDDDDDDTGVLVILTSIRPNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILL 162
           DDD++              P PSELYGK+TWKI+KFSQLN+RE+RSN FEVG YKWYIL+
Sbjct: 132 DDDEE------------AGPKPSELYGKHTWKIDKFSQLNKRELRSNAFEVGGYKWYILI 191

Query: 163 YPRGCDDARGRNHLSLFLSVANHGNLLAGWSHFAQFTMSVMNKDPKKSKYS--------- 222
           YP+GCD     NHLSLFL VANH  LL GWSHFAQFT++V+NKDPKKSKYS         
Sbjct: 192 YPQGCDVC---NHLSLFLCVANHDKLLPGWSHFAQFTIAVVNKDPKKSKYSDTLHRFWKK 251

Query: 223 ----GW----------DG-------------------ADRPFRCLDRQYRRKLIRVYLTN 282
               GW          DG                   ADRPFRCLD QYRR+L+RVYLTN
Sbjct: 252 EHDWGWKKFMELSKVLDGFIDADTLIIKAQVQVIRERADRPFRCLDCQYRRELVRVYLTN 311

Query: 283 VEQISQRFVQERRRKLRKLIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVVKHF 342
           VEQI +RFV+ERR KL KLIEDKA+WSSFR FWL  DQN+R+ MSREKT+ +LKVVVKHF
Sbjct: 312 VEQICRRFVEERRSKLGKLIEDKARWSSFRAFWLAIDQNSRRRMSREKTDTILKVVVKHF 371

Query: 343 FIEREVTSTLVMDSLYSGLKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVDDVL 402
           FIE+EVTSTLVMDSLYSGLKALEGH+K KKGKAKLLD+EEMPAPIVHIEKDTFVLVDDVL
Sbjct: 372 FIEKEVTSTLVMDSLYSGLKALEGHSKSKKGKAKLLDAEEMPAPIVHIEKDTFVLVDDVL 431

Query: 403 LLLERTAMESLPPKDE-DPQNHTKAGSS-KDFDTDSTERDERRFTESGRRNLEIFVLDHI 460
           LLLER A+E LPPK+E  PQN TK GSS +DF+ D+ ERDERR TE GRR +EIFVL HI
Sbjct: 432 LLLERAAVEPLPPKEEKGPQNRTKDGSSGEDFNKDTIERDERRLTELGRRTVEIFVLAHI 491

BLAST of Spg031277 vs. NCBI nr
Match: KAG6582362.1 (TNF receptor-associated factor-like 1b, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 550.1 bits (1416), Expect = 1.9e-152
Identity = 307/461 (66.59%), Postives = 347/461 (75.27%), Query Frame = 0

Query: 43  ISSERKMDSIVGDEAEQIRSMESIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTD 102
           +SSERKM   V +EA  +RS+ES  N   S SGEALAEW+  EQVENG PST+  Y DTD
Sbjct: 58  LSSERKMAGAVSEEALGMRSVESFANGHHSQSGEALAEWRSSEQVENGTPSTSPPYWDTD 117

Query: 103 DDDDDDDDTGVLVILTSIRPNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILL 162
           DDD++              P PSELYGK+TWKI+KFSQLN+RE+RSN FEVG YKWYIL+
Sbjct: 118 DDDEE------------AGPKPSELYGKHTWKIDKFSQLNKRELRSNAFEVGGYKWYILI 177

Query: 163 YPRGCDDARGRNHLSLFLSVANHGNLLAGWSHFAQFTMSVMNKDPKKSKYS--------- 222
           YP+GCD     NHLSLFL VANH  LL GWSHFAQFT++V+NKDPKKSKYS         
Sbjct: 178 YPQGCDVC---NHLSLFLCVANHDKLLPGWSHFAQFTIAVVNKDPKKSKYSDTLHRFWKK 237

Query: 223 ----GW----------DG-------------------ADRPFRCLDRQYRRKLIRVYLTN 282
               GW          DG                   ADRPFRCLD QYRR+L+RVYLTN
Sbjct: 238 EHDWGWKKFMELSKVLDGFIDADTLIIKAQVQVIRERADRPFRCLDCQYRRELVRVYLTN 297

Query: 283 VEQISQRFVQERRRKLRKLIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVVKHF 342
           VEQI +RFV+ERR KL KLIEDKA+WSSFR FWL  DQN+R+ MSREKT+ +LKVVVKHF
Sbjct: 298 VEQICRRFVEERRSKLGKLIEDKARWSSFRAFWLAIDQNSRRRMSREKTDTILKVVVKHF 357

Query: 343 FIEREVTSTLVMDSLYSGLKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVDDVL 402
           FIE+EVTSTLVMDSLYSGLKALEGH+K KKGKAKLLD+EEMPAPIVHIEKDTFVLVDDVL
Sbjct: 358 FIEKEVTSTLVMDSLYSGLKALEGHSKSKKGKAKLLDAEEMPAPIVHIEKDTFVLVDDVL 417

Query: 403 LLLERTAMESLPPKDE-DPQNHTKAGSS-KDFDTDSTERDERRFTESGRRNLEIFVLDHI 460
           LLLER A+E LPPK+E  PQN TK GSS +DF+ D+ ERDERR TE GRR +EIFVL HI
Sbjct: 418 LLLERAAVEPLPPKEEKGPQNRTKDGSSGEDFNKDTIERDERRLTELGRRTVEIFVLAHI 477

BLAST of Spg031277 vs. ExPASy Swiss-Prot
Match: Q8RY18 (TNF receptor-associated factor homolog 1a OS=Arabidopsis thaliana OX=3702 GN=TRAF1A PE=1 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 9.4e-123
Identity = 246/443 (55.53%), Postives = 303/443 (68.40%), Query Frame = 0

Query: 62  SMESIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTDDDDDDDDDTGVLVILTSIR 121
           S+E   N  RS S EA+AEW+  EQVENG PST+  Y D DDDDD               
Sbjct: 15  SLEENSNGQRSQSEEAIAEWRSSEQVENGTPSTSPPYWDIDDDDD-------------FG 74

Query: 122 PNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGCDDARGRNHLSLFLS 181
             PS+L+GK TW IEKFS +N+RE+R + FEVG YKWYIL+YP+GCD     NHLSLFL 
Sbjct: 75  SKPSQLFGKNTWTIEKFSDINKRELRGDVFEVGGYKWYILIYPQGCDVC---NHLSLFLC 134

Query: 182 VANHGNLLAGWSHFAQFTMSVMNKDPKKSKYS-------------GW------------- 241
           VA+H  LL GWSHFAQFT++V NKDPKKSK+S             GW             
Sbjct: 135 VAHHEKLLPGWSHFAQFTIAVSNKDPKKSKHSDTLHRFWKKEHDWGWKKFIELPKLKEGF 194

Query: 242 -----------------DGADRPFRCLDRQYRRKLIRVYLTNVEQISQRFVQERRRKLRK 301
                            +  DRPFRCL  +YR +L+RVYL NVEQI  RFV+E+R KL +
Sbjct: 195 IDDSGCLTIKAQVQVIRERVDRPFRCLHYKYREELVRVYLGNVEQICWRFVEEKRSKLGR 254

Query: 302 LIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVVKHFFIEREVTSTLVMDSLYSG 361
           LIEDKAKW SF  FW+G DQN+R+ MSREK + +LK+VVKHFF+E+EVTSTLVMDSLYSG
Sbjct: 255 LIEDKAKWKSFCAFWMGLDQNSRRRMSREKMDVILKIVVKHFFVEKEVTSTLVMDSLYSG 314

Query: 362 LKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVDDVLLLLERTAMESLPPKDE-D 421
           LKALEG  K K+ + +L+D+EE  APIV ++KD+F LVDDVLLLLE+ A+E LP K+E  
Sbjct: 315 LKALEGQNKNKESRPRLMDTEESTAPIVSVDKDSFALVDDVLLLLEKAALEPLPKKEEKS 374

Query: 422 PQNHTKAGSS-KDFDTDSTERDERRFTESGRRNLEIFVLDHIFSSKIEVSYLEAVALKRK 460
            QN TK G++ ++F  ++ ERD+RR TE GRR +EIFVL HIFS+KIEV+Y EA+A KR+
Sbjct: 375 SQNRTKDGNAGEEFSREAVERDDRRLTELGRRTVEIFVLAHIFSNKIEVAYQEAIAWKRQ 434

BLAST of Spg031277 vs. ExPASy Swiss-Prot
Match: A8MQL1 (TNF receptor-associated factor homolog 1b OS=Arabidopsis thaliana OX=3702 GN=TRAF1B PE=1 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 1.0e-121
Identity = 252/464 (54.31%), Postives = 315/464 (67.89%), Query Frame = 0

Query: 49  MDSIVGDEAEQIRSMESIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTDDDDDDD 108
           M   V +++   RS+E   N   S +GEAL+EW+   QVENG PST+ SY D DDDDD  
Sbjct: 1   MAEAVDEDSGVGRSLEESSNGQHSQAGEALSEWRSSGQVENGTPSTSPSYWDIDDDDD-- 60

Query: 109 DDTGVLVILTSIRPNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGCD 168
                          PSELYG+YTWKI KFS++ +RE RSN FE G YKWYIL+YP+GCD
Sbjct: 61  -----------YGLKPSELYGQYTWKIPKFSEITKREHRSNVFEAGGYKWYILIYPQGCD 120

Query: 169 DARGRNHLSLFLSVANHGNLL--------AGWSHFAQFTMSVMNKDPKKSKYS------- 228
                NHLSLFL VAN+  LL        AGWS FAQFT+SV+++D KKSK+S       
Sbjct: 121 VC---NHLSLFLCVANYDKLLPGSFAILEAGWSQFAQFTISVLSQDLKKSKFSDTLHRFW 180

Query: 229 ------GW----------DG--------------------ADRPFRCLDRQYRRKLIRVY 288
                 GW          DG                     DRPFRCLD  YRR+L+RVY
Sbjct: 181 KKEHDWGWKKFMELPKLKDGFIDESGCLTIEAKVQVIRERVDRPFRCLDCGYRRELVRVY 240

Query: 289 LTNVEQISQRFVQERRRKLRKLIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVV 348
             NVEQI +RFV+E+R KL +LIEDKA+W+SF  FWLG DQN+R+ M REK + +LK VV
Sbjct: 241 FQNVEQICRRFVEEKRSKLGRLIEDKARWTSFGVFWLGMDQNSRRRMCREKVDVILKGVV 300

Query: 349 KHFFIEREVTSTLVMDSLYSGLKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVD 408
           KHFF+E+EV+STLVMDSLYSGLKALEG TK  K +++LLD++++PAPIV ++KD FVLVD
Sbjct: 301 KHFFVEKEVSSTLVMDSLYSGLKALEGQTKNMKARSRLLDAKQLPAPIVSVDKDMFVLVD 360

Query: 409 DVLLLLERTAMESLPPKDE-DPQNHTKAGS-SKDFDTDSTERDERRFTESGRRNLEIFVL 460
           DVLLLLER A+E LPPKDE   QN TK G+  ++ + ++ ERDERR TE GRR +EIF+L
Sbjct: 361 DVLLLLERAALEPLPPKDEKGRQNRTKDGNDGEEVNKEADERDERRLTELGRRTVEIFIL 420

BLAST of Spg031277 vs. ExPASy Swiss-Prot
Match: Q84WU2 (Ubiquitin C-terminal hydrolase 13 OS=Arabidopsis thaliana OX=3702 GN=UBP13 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.0e-12
Identity = 34/76 (44.74%), Postives = 53/76 (69.74%), Query Frame = 0

Query: 130 KYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGCDDARGRNHLSLFLSVANHGNLL 189
           K+TW I  F++LN R+  S+ F VG YKW IL++P+G       +HLS++L VA+  NL 
Sbjct: 55  KFTWTIPMFTRLNTRKHYSDVFVVGGYKWRILIFPKG----NNVDHLSMYLDVADAANLP 114

Query: 190 AGWSHFAQFTMSVMNK 206
            GWS ++QF+++V+N+
Sbjct: 115 YGWSRYSQFSLAVVNQ 126

BLAST of Spg031277 vs. ExPASy Swiss-Prot
Match: Q9FPT1 (Ubiquitin C-terminal hydrolase 12 OS=Arabidopsis thaliana OX=3702 GN=UBP12 PE=1 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 6.8e-12
Identity = 33/76 (43.42%), Postives = 52/76 (68.42%), Query Frame = 0

Query: 130 KYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGCDDARGRNHLSLFLSVANHGNLL 189
           K+TW I  FS+ N R+  S+ F VG YKW IL++P+G       +HLS++L V++  +L 
Sbjct: 56  KFTWTIPNFSRQNTRKHYSDVFVVGGYKWRILIFPKG----NNVDHLSMYLDVSDAASLP 115

Query: 190 AGWSHFAQFTMSVMNK 206
            GWS +AQF+++V+N+
Sbjct: 116 YGWSRYAQFSLAVVNQ 127

BLAST of Spg031277 vs. ExPASy Swiss-Prot
Match: Q9M2J5 (MATH domain and coiled-coil domain-containing protein At3g58210 OS=Arabidopsis thaliana OX=3702 GN=At3g58210 PE=4 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 5.7e-11
Identity = 33/80 (41.25%), Postives = 50/80 (62.50%), Query Frame = 0

Query: 130 KYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGCDDARGRNHLSLFLSVANHGNLL 189
           K+TW I+ FS    R V SN F +G  KW +L+YP G +  +  +HLSLFL VA+  +L 
Sbjct: 8   KFTWVIQNFSSSQSRVVPSNQFVIGGCKWRLLVYPEGFN--KSGDHLSLFLEVADPRSLP 67

Query: 190 AGWSHFAQFTMSVMNKDPKK 210
            GWS  A++ ++++N+   K
Sbjct: 68  PGWSRHARYLLTIVNQHSDK 85

BLAST of Spg031277 vs. ExPASy TrEMBL
Match: A0A6J1DPS9 (MATH domain-containing protein At5g43560-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021962 PE=4 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 1.3e-154
Identity = 312/456 (68.42%), Postives = 346/456 (75.88%), Query Frame = 0

Query: 49  MDSIVGDEAEQIRSME-SIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTDDDDDD 108
           M S+V +EAE +RS++ SI N   S  GEALAEW+  EQVENG PST+  Y DTDDDDDD
Sbjct: 1   MASVVSEEAEGMRSVDSSIANGHHSQPGEALAEWRSSEQVENGTPSTSPPYWDTDDDDDD 60

Query: 109 DDDTGVLVILTSIRPNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGC 168
                         P PSELYGK+TWKIEKFSQLN+RE+RSN FEVG YKWYIL+YP+GC
Sbjct: 61  ------------AGPKPSELYGKHTWKIEKFSQLNKRELRSNAFEVGGYKWYILIYPQGC 120

Query: 169 DDARGRNHLSLFLSVANHGNLLAGWSHFAQFTMSVMNKDPKKSKYS-------------G 228
           D     NHLSLFL VANH  LL GWSHFAQFT++V+NKDPKKSKYS             G
Sbjct: 121 DVC---NHLSLFLCVANHDKLLPGWSHFAQFTIAVVNKDPKKSKYSDTLHRFWKKEHDWG 180

Query: 229 W----------DG-------------------ADRPFRCLDRQYRRKLIRVYLTNVEQIS 288
           W          DG                   ADRPFRCLD QYRR+L+RVYLTNVEQI 
Sbjct: 181 WKKFMELSKVLDGFIDADTLIIKAQVQVIRERADRPFRCLDCQYRRELVRVYLTNVEQIC 240

Query: 289 QRFVQERRRKLRKLIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVVKHFFIERE 348
           +RFV+ERR KL KLIEDKA+WSSFR FWLG DQNAR+ MSREKT+ +LKVVVKHFFIE+E
Sbjct: 241 RRFVEERRGKLGKLIEDKARWSSFRAFWLGIDQNARRRMSREKTDTILKVVVKHFFIEKE 300

Query: 349 VTSTLVMDSLYSGLKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVDDVLLLLER 408
           VTSTLVMDSLYSGLKALEGHTKCKKGKAKLLD+EEMPAPIVHIEKDTFVLVDDVLLLLER
Sbjct: 301 VTSTLVMDSLYSGLKALEGHTKCKKGKAKLLDAEEMPAPIVHIEKDTFVLVDDVLLLLER 360

Query: 409 TAMESLPPKDE-DPQNHTKAGSS-KDFDTDSTERDERRFTESGRRNLEIFVLDHIFSSKI 460
            +ME LPPKDE  PQN TK GSS +DF+ DS ERDERR TE GRR +EIFVL HIFSSK+
Sbjct: 361 ASMEPLPPKDEKGPQNRTKDGSSGEDFNKDSIERDERRLTELGRRTVEIFVLAHIFSSKV 420

BLAST of Spg031277 vs. ExPASy TrEMBL
Match: A0A6J1DPU0 (MATH domain-containing protein At5g43560-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021962 PE=4 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 9.1e-153
Identity = 311/456 (68.20%), Postives = 345/456 (75.66%), Query Frame = 0

Query: 49  MDSIVGDEAEQIRSME-SIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTDDDDDD 108
           M S+V +EAE +RS++ SI N   S  GEALAEW+  EQVENG PST+  Y DTDDDDDD
Sbjct: 1   MASVVSEEAEGMRSVDSSIANGHHSQPGEALAEWRSSEQVENGTPSTSPPYWDTDDDDDD 60

Query: 109 DDDTGVLVILTSIRPNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGC 168
                         P PSELYGK+TWKIEKFSQLN+RE+RSN FEVG YKWYIL+YP+GC
Sbjct: 61  ------------AGPKPSELYGKHTWKIEKFSQLNKRELRSNAFEVGGYKWYILIYPQGC 120

Query: 169 DDARGRNHLSLFLSVANHGNLLAGWSHFAQFTMSVMNKDPKKSKYS-------------G 228
           D     NHLSLFL VANH  LL GWSHFAQFT++V+NKDPKKSKYS             G
Sbjct: 121 DVC---NHLSLFLCVANHDKLLPGWSHFAQFTIAVVNKDPKKSKYSDTLHRFWKKEHDWG 180

Query: 229 W----------DG-------------------ADRPFRCLDRQYRRKLIRVYLTNVEQIS 288
           W          DG                   ADRPFRCLD QYRR+L+RVYLTNVEQI 
Sbjct: 181 WKKFMELSKVLDGFIDADTLIIKAQVQVIRERADRPFRCLDCQYRRELVRVYLTNVEQIC 240

Query: 289 QRFVQERRRKLRKLIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVVKHFFIERE 348
           +RFV+ERR KL KLIEDKA+WSSFR FWLG DQNAR+ MSREKT+ +LKVVVKHFFIE+E
Sbjct: 241 RRFVEERRGKLGKLIEDKARWSSFRAFWLGIDQNARRRMSREKTDTILKVVVKHFFIEKE 300

Query: 349 VTSTLVMDSLYSGLKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVDDVLLLLER 408
           VTSTLVMDSLYSGLKALEGHTKCKKGKAKLLD+EEMPAPIVHIEKDTFVLVDDVLLLLER
Sbjct: 301 VTSTLVMDSLYSGLKALEGHTKCKKGKAKLLDAEEMPAPIVHIEKDTFVLVDDVLLLLER 360

Query: 409 TAMESLPPKDE-DPQNHTKAGSS-KDFDTDSTERDERRFTESGRRNLEIFVLDHIFSSKI 460
            +ME LPPKDE  PQN TK GSS +DF+ DS ERDERR TE GRR +EIFVL HIF SK+
Sbjct: 361 ASMEPLPPKDEKGPQNRTKDGSSGEDFNKDSIERDERRLTELGRRTVEIFVLAHIF-SKV 420

BLAST of Spg031277 vs. ExPASy TrEMBL
Match: A0A1S3AWT4 (MATH domain-containing protein At5g43560 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483634 PE=4 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 1.6e-152
Identity = 306/455 (67.25%), Postives = 343/455 (75.38%), Query Frame = 0

Query: 49  MDSIVGDEAEQIRSMESIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTDDDDDDD 108
           M  +V ++A  +RS+ES  N   S SGEALAEW+  EQVENG PST+  Y DTDDDDDD 
Sbjct: 1   MAGVVSEDAVGVRSVESFANGHHSQSGEALAEWRSSEQVENGTPSTSPPYWDTDDDDDD- 60

Query: 109 DDTGVLVILTSIRPNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGCD 168
                        P PS+LYGK+TWKIEKFSQL +RE+RSN FEVG YKWYIL+YP+GCD
Sbjct: 61  -----------AGPKPSDLYGKHTWKIEKFSQLTKRELRSNAFEVGGYKWYILIYPQGCD 120

Query: 169 DARGRNHLSLFLSVANHGNLLAGWSHFAQFTMSVMNKDPKKSKYS-------------GW 228
                NHLSLFL VANH  LL GWSHFAQFT++V+NKDPKKSKYS             GW
Sbjct: 121 VC---NHLSLFLCVANHDKLLPGWSHFAQFTIAVVNKDPKKSKYSDTLHRFWKKEHDWGW 180

Query: 229 ----------DG-------------------ADRPFRCLDRQYRRKLIRVYLTNVEQISQ 288
                     DG                   ADRPFRCLD QYRR+L+RVYLTNVEQI +
Sbjct: 181 KKFMELSKVLDGFIDADTLIIKAQVQVIRERADRPFRCLDCQYRRELVRVYLTNVEQICR 240

Query: 289 RFVQERRRKLRKLIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVVKHFFIEREV 348
           RFV+ERR KL KLIEDKA+WSSFR FWLG DQNAR+ MSREKT+A+LKVVVKHFFIE+EV
Sbjct: 241 RFVEERRSKLGKLIEDKARWSSFRAFWLGIDQNARRRMSREKTDAILKVVVKHFFIEKEV 300

Query: 349 TSTLVMDSLYSGLKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVDDVLLLLERT 408
           TSTLVMDSLYSGLKALEGHTK KKGKAKLLD+EE+ APIVHIEKDTFVLVDDVLLLLER 
Sbjct: 301 TSTLVMDSLYSGLKALEGHTKSKKGKAKLLDTEEITAPIVHIEKDTFVLVDDVLLLLERA 360

Query: 409 AMESLPPKDE-DPQNHTKAGSS-KDFDTDSTERDERRFTESGRRNLEIFVLDHIFSSKIE 460
           A+E LPPKDE  PQN TK GSS +DF+ DS ERDERR TE GRR +EIFVL HIFSSK+E
Sbjct: 361 AVEPLPPKDEKGPQNRTKDGSSGEDFNKDSIERDERRLTELGRRTVEIFVLAHIFSSKVE 420

BLAST of Spg031277 vs. ExPASy TrEMBL
Match: A0A5A7U1S6 (MATH domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G005310 PE=4 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 1.6e-152
Identity = 306/455 (67.25%), Postives = 343/455 (75.38%), Query Frame = 0

Query: 49  MDSIVGDEAEQIRSMESIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTDDDDDDD 108
           M  +V ++A  +RS+ES  N   S SGEALAEW+  EQVENG PST+  Y DTDDDDDD 
Sbjct: 1   MAGVVSEDAVGVRSVESFANGHHSQSGEALAEWRSSEQVENGTPSTSPPYWDTDDDDDD- 60

Query: 109 DDTGVLVILTSIRPNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGCD 168
                        P PS+LYGK+TWKIEKFSQL +RE+RSN FEVG YKWYIL+YP+GCD
Sbjct: 61  -----------AGPKPSDLYGKHTWKIEKFSQLTKRELRSNAFEVGGYKWYILIYPQGCD 120

Query: 169 DARGRNHLSLFLSVANHGNLLAGWSHFAQFTMSVMNKDPKKSKYS-------------GW 228
                NHLSLFL VANH  LL GWSHFAQFT++V+NKDPKKSKYS             GW
Sbjct: 121 VC---NHLSLFLCVANHDKLLPGWSHFAQFTIAVVNKDPKKSKYSDTLHRFWKKEHDWGW 180

Query: 229 ----------DG-------------------ADRPFRCLDRQYRRKLIRVYLTNVEQISQ 288
                     DG                   ADRPFRCLD QYRR+L+RVYLTNVEQI +
Sbjct: 181 KKFMELSKVLDGFIDADTLIIKAQVQVIRERADRPFRCLDCQYRRELVRVYLTNVEQICR 240

Query: 289 RFVQERRRKLRKLIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVVKHFFIEREV 348
           RFV+ERR KL KLIEDKA+WSSFR FWLG DQNAR+ MSREKT+A+LKVVVKHFFIE+EV
Sbjct: 241 RFVEERRSKLGKLIEDKARWSSFRAFWLGIDQNARRRMSREKTDAILKVVVKHFFIEKEV 300

Query: 349 TSTLVMDSLYSGLKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVDDVLLLLERT 408
           TSTLVMDSLYSGLKALEGHTK KKGKAKLLD+EE+ APIVHIEKDTFVLVDDVLLLLER 
Sbjct: 301 TSTLVMDSLYSGLKALEGHTKSKKGKAKLLDTEEITAPIVHIEKDTFVLVDDVLLLLERA 360

Query: 409 AMESLPPKDE-DPQNHTKAGSS-KDFDTDSTERDERRFTESGRRNLEIFVLDHIFSSKIE 460
           A+E LPPKDE  PQN TK GSS +DF+ DS ERDERR TE GRR +EIFVL HIFSSK+E
Sbjct: 361 AVEPLPPKDEKGPQNRTKDGSSGEDFNKDSIERDERRLTELGRRTVEIFVLAHIFSSKVE 420

BLAST of Spg031277 vs. ExPASy TrEMBL
Match: A0A6J1F6T4 (MATH domain-containing protein At5g43560-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442685 PE=4 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 5.0e-151
Identity = 305/455 (67.03%), Postives = 343/455 (75.38%), Query Frame = 0

Query: 49  MDSIVGDEAEQIRSMESIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTDDDDDDD 108
           M   V +EA  +RS+ES  N   S SGEALAEW+  EQVENG PST+  Y DTDD+++++
Sbjct: 1   MAGAVSEEAIGMRSVESFANGHHSQSGEALAEWRSSEQVENGTPSTSPPYWDTDDEEEEE 60

Query: 109 DDTGVLVILTSIRPNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGCD 168
                        P PSELYGK+TWKIEKFSQLN+RE+RSN FEVG YKWYIL+YP+GCD
Sbjct: 61  -----------AGPKPSELYGKHTWKIEKFSQLNKRELRSNAFEVGGYKWYILIYPQGCD 120

Query: 169 DARGRNHLSLFLSVANHGNLLAGWSHFAQFTMSVMNKDPKKSKYS-------------GW 228
                NHLSLFL VANH  LL GWSHFAQFT++V+NKDPKKSKYS             GW
Sbjct: 121 VC---NHLSLFLCVANHDKLLPGWSHFAQFTIAVVNKDPKKSKYSDTLHRFWKKEHDWGW 180

Query: 229 ----------DG-------------------ADRPFRCLDRQYRRKLIRVYLTNVEQISQ 288
                     DG                   ADRPFRCLD QYRR+L+RVYLTNVEQI +
Sbjct: 181 KKFMELSKVLDGFIDSDTLIIKAQVQVIREKADRPFRCLDCQYRRELVRVYLTNVEQICR 240

Query: 289 RFVQERRRKLRKLIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVVKHFFIEREV 348
           RFV+ERR KL KLI DKA+WSSFR FWLG DQNAR+ MSRE+T+A+LKVVVKHFFIE+EV
Sbjct: 241 RFVEERRSKLGKLIADKARWSSFRAFWLGIDQNARRRMSRERTDAILKVVVKHFFIEKEV 300

Query: 349 TSTLVMDSLYSGLKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVDDVLLLLERT 408
           TSTLVMDSLYSGLKALEGHTK KKGKAKLLDSEEMPAPIVHI+KDTFVLVDDVL LLER 
Sbjct: 301 TSTLVMDSLYSGLKALEGHTKNKKGKAKLLDSEEMPAPIVHIDKDTFVLVDDVLSLLERA 360

Query: 409 AMESLPPKDE-DPQNHTKAGSS-KDFDTDSTERDERRFTESGRRNLEIFVLDHIFSSKIE 460
           A+E LPPKDE  PQN TK GSS +DF+ DS ERDERR TE GRR +EIFVL HIFSSK+E
Sbjct: 361 AVEPLPPKDEKGPQNRTKDGSSGEDFNKDSIERDERRLTELGRRTVEIFVLAHIFSSKVE 420

BLAST of Spg031277 vs. TAIR 10
Match: AT1G04300.1 (TRAF-like superfamily protein )

HSP 1 Score: 443.7 bits (1140), Expect = 1.8e-124
Identity = 251/456 (55.04%), Postives = 314/456 (68.86%), Query Frame = 0

Query: 49  MDSIVGDEAEQIRSMESIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTDDDDDDD 108
           M   V +++   RS+E   N   S +GEAL+EW+   QVENG PST+ SY D DDDDD  
Sbjct: 1   MAEAVDEDSGVGRSLEESSNGQHSQAGEALSEWRSSGQVENGTPSTSPSYWDIDDDDD-- 60

Query: 109 DDTGVLVILTSIRPNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGCD 168
                          PSELYG+YTWKI KFS++ +RE RSN FE G YKWYIL+YP+GCD
Sbjct: 61  -----------YGLKPSELYGQYTWKIPKFSEITKREHRSNVFEAGGYKWYILIYPQGCD 120

Query: 169 DARGRNHLSLFLSVANHGNLLAGWSHFAQFTMSVMNKDPKKSKYS-------------GW 228
                NHLSLFL VAN+  LL GWS FAQFT+SV+++D KKSK+S             GW
Sbjct: 121 VC---NHLSLFLCVANYDKLLPGWSQFAQFTISVLSQDLKKSKFSDTLHRFWKKEHDWGW 180

Query: 229 ----------DG--------------------ADRPFRCLDRQYRRKLIRVYLTNVEQIS 288
                     DG                     DRPFRCLD  YRR+L+RVY  NVEQI 
Sbjct: 181 KKFMELPKLKDGFIDESGCLTIEAKVQVIRERVDRPFRCLDCGYRRELVRVYFQNVEQIC 240

Query: 289 QRFVQERRRKLRKLIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVVKHFFIERE 348
           +RFV+E+R KL +LIEDKA+W+SF  FWLG DQN+R+ M REK + +LK VVKHFF+E+E
Sbjct: 241 RRFVEEKRSKLGRLIEDKARWTSFGVFWLGMDQNSRRRMCREKVDVILKGVVKHFFVEKE 300

Query: 349 VTSTLVMDSLYSGLKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVDDVLLLLER 408
           V+STLVMDSLYSGLKALEG TK  K +++LLD++++PAPIV ++KD FVLVDDVLLLLER
Sbjct: 301 VSSTLVMDSLYSGLKALEGQTKNMKARSRLLDAKQLPAPIVSVDKDMFVLVDDVLLLLER 360

Query: 409 TAMESLPPKDE-DPQNHTKAGS-SKDFDTDSTERDERRFTESGRRNLEIFVLDHIFSSKI 460
            A+E LPPKDE   QN TK G+  ++ + ++ ERDERR TE GRR +EIF+L HIFS+KI
Sbjct: 361 AALEPLPPKDEKGRQNRTKDGNDGEEVNKEADERDERRLTELGRRTVEIFILSHIFSTKI 420

BLAST of Spg031277 vs. TAIR 10
Match: AT5G43560.1 (TRAF-like superfamily protein )

HSP 1 Score: 441.8 bits (1135), Expect = 6.7e-124
Identity = 246/443 (55.53%), Postives = 303/443 (68.40%), Query Frame = 0

Query: 62  SMESIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTDDDDDDDDDTGVLVILTSIR 121
           S+E   N  RS S EA+AEW+  EQVENG PST+  Y D DDDDD               
Sbjct: 15  SLEENSNGQRSQSEEAIAEWRSSEQVENGTPSTSPPYWDIDDDDD-------------FG 74

Query: 122 PNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGCDDARGRNHLSLFLS 181
             PS+L+GK TW IEKFS +N+RE+R + FEVG YKWYIL+YP+GCD     NHLSLFL 
Sbjct: 75  SKPSQLFGKNTWTIEKFSDINKRELRGDVFEVGGYKWYILIYPQGCDVC---NHLSLFLC 134

Query: 182 VANHGNLLAGWSHFAQFTMSVMNKDPKKSKYS-------------GW------------- 241
           VA+H  LL GWSHFAQFT++V NKDPKKSK+S             GW             
Sbjct: 135 VAHHEKLLPGWSHFAQFTIAVSNKDPKKSKHSDTLHRFWKKEHDWGWKKFIELPKLKEGF 194

Query: 242 -----------------DGADRPFRCLDRQYRRKLIRVYLTNVEQISQRFVQERRRKLRK 301
                            +  DRPFRCL  +YR +L+RVYL NVEQI  RFV+E+R KL +
Sbjct: 195 IDDSGCLTIKAQVQVIRERVDRPFRCLHYKYREELVRVYLGNVEQICWRFVEEKRSKLGR 254

Query: 302 LIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVVKHFFIEREVTSTLVMDSLYSG 361
           LIEDKAKW SF  FW+G DQN+R+ MSREK + +LK+VVKHFF+E+EVTSTLVMDSLYSG
Sbjct: 255 LIEDKAKWKSFCAFWMGLDQNSRRRMSREKMDVILKIVVKHFFVEKEVTSTLVMDSLYSG 314

Query: 362 LKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVDDVLLLLERTAMESLPPKDE-D 421
           LKALEG  K K+ + +L+D+EE  APIV ++KD+F LVDDVLLLLE+ A+E LP K+E  
Sbjct: 315 LKALEGQNKNKESRPRLMDTEESTAPIVSVDKDSFALVDDVLLLLEKAALEPLPKKEEKS 374

Query: 422 PQNHTKAGSS-KDFDTDSTERDERRFTESGRRNLEIFVLDHIFSSKIEVSYLEAVALKRK 460
            QN TK G++ ++F  ++ ERD+RR TE GRR +EIFVL HIFS+KIEV+Y EA+A KR+
Sbjct: 375 SQNRTKDGNAGEEFSREAVERDDRRLTELGRRTVEIFVLAHIFSNKIEVAYQEAIAWKRQ 434

BLAST of Spg031277 vs. TAIR 10
Match: AT5G43560.2 (TRAF-like superfamily protein )

HSP 1 Score: 441.8 bits (1135), Expect = 6.7e-124
Identity = 246/443 (55.53%), Postives = 303/443 (68.40%), Query Frame = 0

Query: 62  SMESIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTDDDDDDDDDTGVLVILTSIR 121
           S+E   N  RS S EA+AEW+  EQVENG PST+  Y D DDDDD               
Sbjct: 15  SLEENSNGQRSQSEEAIAEWRSSEQVENGTPSTSPPYWDIDDDDD-------------FG 74

Query: 122 PNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGCDDARGRNHLSLFLS 181
             PS+L+GK TW IEKFS +N+RE+R + FEVG YKWYIL+YP+GCD     NHLSLFL 
Sbjct: 75  SKPSQLFGKNTWTIEKFSDINKRELRGDVFEVGGYKWYILIYPQGCDVC---NHLSLFLC 134

Query: 182 VANHGNLLAGWSHFAQFTMSVMNKDPKKSKYS-------------GW------------- 241
           VA+H  LL GWSHFAQFT++V NKDPKKSK+S             GW             
Sbjct: 135 VAHHEKLLPGWSHFAQFTIAVSNKDPKKSKHSDTLHRFWKKEHDWGWKKFIELPKLKEGF 194

Query: 242 -----------------DGADRPFRCLDRQYRRKLIRVYLTNVEQISQRFVQERRRKLRK 301
                            +  DRPFRCL  +YR +L+RVYL NVEQI  RFV+E+R KL +
Sbjct: 195 IDDSGCLTIKAQVQVIRERVDRPFRCLHYKYREELVRVYLGNVEQICWRFVEEKRSKLGR 254

Query: 302 LIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVVKHFFIEREVTSTLVMDSLYSG 361
           LIEDKAKW SF  FW+G DQN+R+ MSREK + +LK+VVKHFF+E+EVTSTLVMDSLYSG
Sbjct: 255 LIEDKAKWKSFCAFWMGLDQNSRRRMSREKMDVILKIVVKHFFVEKEVTSTLVMDSLYSG 314

Query: 362 LKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVDDVLLLLERTAMESLPPKDE-D 421
           LKALEG  K K+ + +L+D+EE  APIV ++KD+F LVDDVLLLLE+ A+E LP K+E  
Sbjct: 315 LKALEGQNKNKESRPRLMDTEESTAPIVSVDKDSFALVDDVLLLLEKAALEPLPKKEEKS 374

Query: 422 PQNHTKAGSS-KDFDTDSTERDERRFTESGRRNLEIFVLDHIFSSKIEVSYLEAVALKRK 460
            QN TK G++ ++F  ++ ERD+RR TE GRR +EIFVL HIFS+KIEV+Y EA+A KR+
Sbjct: 375 SQNRTKDGNAGEEFSREAVERDDRRLTELGRRTVEIFVLAHIFSNKIEVAYQEAIAWKRQ 434

BLAST of Spg031277 vs. TAIR 10
Match: AT1G04300.3 (TRAF-like superfamily protein )

HSP 1 Score: 438.3 bits (1126), Expect = 7.4e-123
Identity = 252/464 (54.31%), Postives = 315/464 (67.89%), Query Frame = 0

Query: 49  MDSIVGDEAEQIRSMESIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTDDDDDDD 108
           M   V +++   RS+E   N   S +GEAL+EW+   QVENG PST+ SY D DDDDD  
Sbjct: 1   MAEAVDEDSGVGRSLEESSNGQHSQAGEALSEWRSSGQVENGTPSTSPSYWDIDDDDD-- 60

Query: 109 DDTGVLVILTSIRPNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGCD 168
                          PSELYG+YTWKI KFS++ +RE RSN FE G YKWYIL+YP+GCD
Sbjct: 61  -----------YGLKPSELYGQYTWKIPKFSEITKREHRSNVFEAGGYKWYILIYPQGCD 120

Query: 169 DARGRNHLSLFLSVANHGNLL--------AGWSHFAQFTMSVMNKDPKKSKYS------- 228
                NHLSLFL VAN+  LL        AGWS FAQFT+SV+++D KKSK+S       
Sbjct: 121 VC---NHLSLFLCVANYDKLLPGSFAILEAGWSQFAQFTISVLSQDLKKSKFSDTLHRFW 180

Query: 229 ------GW----------DG--------------------ADRPFRCLDRQYRRKLIRVY 288
                 GW          DG                     DRPFRCLD  YRR+L+RVY
Sbjct: 181 KKEHDWGWKKFMELPKLKDGFIDESGCLTIEAKVQVIRERVDRPFRCLDCGYRRELVRVY 240

Query: 289 LTNVEQISQRFVQERRRKLRKLIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVV 348
             NVEQI +RFV+E+R KL +LIEDKA+W+SF  FWLG DQN+R+ M REK + +LK VV
Sbjct: 241 FQNVEQICRRFVEEKRSKLGRLIEDKARWTSFGVFWLGMDQNSRRRMCREKVDVILKGVV 300

Query: 349 KHFFIEREVTSTLVMDSLYSGLKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVD 408
           KHFF+E+EV+STLVMDSLYSGLKALEG TK  K +++LLD++++PAPIV ++KD FVLVD
Sbjct: 301 KHFFVEKEVSSTLVMDSLYSGLKALEGQTKNMKARSRLLDAKQLPAPIVSVDKDMFVLVD 360

Query: 409 DVLLLLERTAMESLPPKDE-DPQNHTKAGS-SKDFDTDSTERDERRFTESGRRNLEIFVL 460
           DVLLLLER A+E LPPKDE   QN TK G+  ++ + ++ ERDERR TE GRR +EIF+L
Sbjct: 361 DVLLLLERAALEPLPPKDEKGRQNRTKDGNDGEEVNKEADERDERRLTELGRRTVEIFIL 420

BLAST of Spg031277 vs. TAIR 10
Match: AT1G04300.4 (TRAF-like superfamily protein )

HSP 1 Score: 406.4 bits (1043), Expect = 3.1e-113
Identity = 237/456 (51.97%), Postives = 299/456 (65.57%), Query Frame = 0

Query: 49  MDSIVGDEAEQIRSMESIVNELRSLSGEALAEWKPFEQVENGMPSTAQSYLDTDDDDDDD 108
           M   V +++   RS+E   N   S +GEAL+EW+   QVENG PST+ SY D DDDDD  
Sbjct: 1   MAEAVDEDSGVGRSLEESSNGQHSQAGEALSEWRSSGQVENGTPSTSPSYWDIDDDDD-- 60

Query: 109 DDTGVLVILTSIRPNPSELYGKYTWKIEKFSQLNRREVRSNTFEVGSYKWYILLYPRGCD 168
                                          ++ +RE RSN FE G YKWYIL+YP+GCD
Sbjct: 61  ------------------------------YEITKREHRSNVFEAGGYKWYILIYPQGCD 120

Query: 169 DARGRNHLSLFLSVANHGNLLAGWSHFAQFTMSVMNKDPKKSKYS-------------GW 228
                NHLSLFL VAN+  LL GWS FAQFT+SV+++D KKSK+S             GW
Sbjct: 121 VC---NHLSLFLCVANYDKLLPGWSQFAQFTISVLSQDLKKSKFSDTLHRFWKKEHDWGW 180

Query: 229 ----------DG--------------------ADRPFRCLDRQYRRKLIRVYLTNVEQIS 288
                     DG                     DRPFRCLD  YRR+L+RVY  NVEQI 
Sbjct: 181 KKFMELPKLKDGFIDESGCLTIEAKVQVIRERVDRPFRCLDCGYRRELVRVYFQNVEQIC 240

Query: 289 QRFVQERRRKLRKLIEDKAKWSSFRDFWLGTDQNARQLMSREKTEAVLKVVVKHFFIERE 348
           +RFV+E+R KL +LIEDKA+W+SF  FWLG DQN+R+ M REK + +LK VVKHFF+E+E
Sbjct: 241 RRFVEEKRSKLGRLIEDKARWTSFGVFWLGMDQNSRRRMCREKVDVILKGVVKHFFVEKE 300

Query: 349 VTSTLVMDSLYSGLKALEGHTKCKKGKAKLLDSEEMPAPIVHIEKDTFVLVDDVLLLLER 408
           V+STLVMDSLYSGLKALEG TK  K +++LLD++++PAPIV ++KD FVLVDDVLLLLER
Sbjct: 301 VSSTLVMDSLYSGLKALEGQTKNMKARSRLLDAKQLPAPIVSVDKDMFVLVDDVLLLLER 360

Query: 409 TAMESLPPKDE-DPQNHTKAGS-SKDFDTDSTERDERRFTESGRRNLEIFVLDHIFSSKI 460
            A+E LPPKDE   QN TK G+  ++ + ++ ERDERR TE GRR +EIF+L HIFS+KI
Sbjct: 361 AALEPLPPKDEKGRQNRTKDGNDGEEVNKEADERDERRLTELGRRTVEIFILSHIFSTKI 420