Spg031173 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg031173
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	cytochrome P450 81D1-like
Location	scaffold8: 30720861 .. 30723549 (-)
RNA-Seq Expression	Spg031173
Synteny	Spg031173

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGGTTTGAAAATTTTACAATTTAGTAGAAACAAATGGAACACACGTCAACAATTGTTATGAAAAGTCTTTGCTTCAAGGAGCTAAACAACAATGTTGAAAAGTTGTGCTCACTTAAGTCTAGAGTCAATACTCTCACTTTCGTGGTTGCTTTCAACTTTTTCATGATACTCCAAAGAAACAAAGCCACAAGCATCTCCATCACCCTCCCCAATTTCATCTCACCTTCCAAAATATCTTCCTTCCTTTTCCCCCAACAAGACTTCGAACCAAAAAAAAAAAAAAAAACTTGACTTTCACTCATAAGAAAGTTGAGTCAAGAAAAATCCCAAGACGAGAAGACAATGAAACTGATCAACAACAATGGATAATAATTTGCTTTACATTTTCCTCTGCCTGATCTCCCTCCTTTTTGCCCTCAAATTTTTGCTGCGCCATGGCAGAAACCTCCCACCAAGCCCACTCTTTTCCCTTCCATTAATAGGCCATTTCCATCTGCTAAGACATCCCATCCACCGCAATCTTCACGAGCTCTCAAAGAAGCATGGCCACGTCTTCTCTCTCTGGTTTGGTTCTCGTCTCGCTGTAGTTGTATCATCTCCTTCCGCCGTTCGTGAATGCTTCACAAAGAACGACATCGTTCTCGCAAATCGCCCATTTCTCAACACTGGAAAGCACTTGGCCTACAACTTTACCGTACTCGCCGTCGCCCCTTACGGCGAGCTTTGGCGTAACCTTCGCCGCATCAGTACTCACGAGATTTTCTCTGCAAGCCGACTTAATTTGTTCTCCGGAATCCGACAAGATGAAGTCAAGCGCTTGTTGTGTAAATTATGCGGCGATTCGGTGGAAGAATTCTCTGTCGTCGAGCTTGAGCCGATGCTTTTGGATCTTACGTCTAACATCGTCATGAGGATGGTCGCCGGAAAGAGGTATTTTGGAGACGACGTGTCGGATGAAGGAAAAGCGAGGCAATTTAGAGATGTTGTGAAGCGAGTTATGTTGTATGCTGGGGTGACAAATCCAGGAGACTTTATACCCATATGGAATTGGATTGATCCAACTGGTCTGGAGAAGAAGATAATGAAGATTGGACAGGAAGCTGATGCAATCTTTCAGGCTTTAATCGATGAGATTCGGAGCCAAAAAGAACGTGGAAATACGATGATTGATCATTTACTTCACTTGCAGAGCTCCCAACCTGAATACTTCAATGACCAAATTATCAAAGGCCTCATACATGTACGTTCTCTTTTTTCCATTCATGAAGTCTCTGATAGGATCATCATCAGTCGGTTGGCGTCGGTTTTTGGCTCAAACTGACACCGACCACCGACAAATCGATTTTGATAGGTCGGTTCTAGTCGATTTTGAGGATGTTTGGTGGGCGAACCGACCGACCATACCGGCGGTCGGTCGGTTTTAGTCGGTTTTCTAGTATTGATGAACGGAAAAAGAAAAGAGAGAAGACAACCAACAAAAAAAAAATAGAGAAGGAGAAGGAGAAGAAAGGAATAGAAAGAAGAAAATGGAATGGGAAAGAAAAAAATGGAATGAAAATAATAGAGAAGAAGAAGACTGTGGGAGAAAGAAAAAGGAATGGAAAAGAGGTAAAAAGAAAAGTGAGAAGAAGAGAAGAAAGAAAGAAAAAAAGAGAAGAGAAAGAAAGGGAGTTCAGACCTTCGCCTTCGGTGATGGAGAAAATAATGGAGAAGAAAGAAATGTGGGAGAAAGTAGATTAAAGAAGAAAGAAAAAAAATAAAAAAAAAAATTAATGGGCCTTGGGGAAAGGGAATGGTTGCAGCAATTTAATGCTTGCAGAGGAATGGTTGCAGCAATGAGCCGCGTGCTGTCAAGTGTCACTTCATCTTTTTTTTTTTTTTAATTTCAGTTGGTTTCGGTCAGTTTGAGCCTTTTTGTGCTCACCGACCGATATAACTAAAAAAACCGACCGACCGATGTCGGTTCGGTCGGTTTCGGTCGGTCGGCTTGGGTTTTCGACCTATGATGCTCACCCCTAGTGTCTGATCTCTCTCTGAAGTACTCGATTTTTGCAGGTTATACTACTGGCAGGGATCGATACAACGGCTGTGACATTAGAATGGGCAGTCTCCCACTTGCTCAACGAACCAGAGGTGATAAAGAAAGCGAGATTTGAGATAGAGAGTTTCACTGGACAAGAACGTTTGGTTAATGAAGATGATTTGTCGAGTTTGAGTTATGTGCAAGGAATAATTTTGGAGACTCTGCGGTTGACCCCAGCAGCTCCTATGCTTGTGCCGCACTGTGCATCTGAAGATTGCAAAATAGAAGGCTATGATGTACCACGTGACACGATTGTGTTTGTTAACGCTTGGGCTATACATAGAGATCCAAACCTATGGGAAGATGTCACTAGTTTTAAGCCTGAAAGGCATGAAACTGCAAATGAGTCGTTTGATTCATACAAACTGTTACCATTCGGGTTGGGGAGGAGGGCATGCCCTGGTGTAGGAATGGCGCAACGTGTGGTTGGCTTGACCTTGGCGTCATTGATTCAATGTTTTGATTGGGAAAGAATGAGTAGTTCATTGGTTGACATGACTGAAGGACAAGGGATCACTATGCCGAAAGCTCAGCCATTGGTAGCCAAGTGTAAACCACGCCCTATTATGAGAGCTCTTGTTAGTGAAATAATTCACCCTTTTTAA

mRNA sequence

ATGGATAATAATTTGCTTTACATTTTCCTCTGCCTGATCTCCCTCCTTTTTGCCCTCAAATTTTTGCTGCGCCATGGCAGAAACCTCCCACCAAGCCCACTCTTTTCCCTTCCATTAATAGGCCATTTCCATCTGCTAAGACATCCCATCCACCGCAATCTTCACGAGCTCTCAAAGAAGCATGGCCACGTCTTCTCTCTCTGGTTTGGTTCTCGTCTCGCTGTAGTTGTATCATCTCCTTCCGCCGTTCGTGAATGCTTCACAAAGAACGACATCGTTCTCGCAAATCGCCCATTTCTCAACACTGGAAAGCACTTGGCCTACAACTTTACCGTACTCGCCGTCGCCCCTTACGGCGAGCTTTGGCGTAACCTTCGCCGCATCAGTACTCACGAGATTTTCTCTGCAAGCCGACTTAATTTGTTCTCCGGAATCCGACAAGATGAAGTCAAGCGCTTGTTGTGTAAATTATGCGGCGATTCGGTGGAAGAATTCTCTGTCGTCGAGCTTGAGCCGATGCTTTTGGATCTTACGTCTAACATCGTCATGAGGATGGTCGCCGGAAAGAGGTATTTTGGAGACGACGTGTCGGATGAAGGAAAAGCGAGGCAATTTAGAGATGTTGTGAAGCGAGTTATGTTGTATGCTGGGGTGACAAATCCAGGAGACTTTATACCCATATGGAATTGGATTGATCCAACTGGTCTGGAGAAGAAGATAATGAAGATTGGACAGGAAGCTGATGCAATCTTTCAGGCTTTAATCGATGAGATTCGGAGCCAAAAAGAACGTGGAAATACGATGATTGATCATTTACTTCACTTGCAGAGCTCCCAACCTGAATACTTCAATGACCAAATTATCAAAGGCCTCATACATGTTATACTACTGGCAGGGATCGATACAACGGCTGTGACATTAGAATGGGCAGTCTCCCACTTGCTCAACGAACCAGAGGTGATAAAGAAAGCGAGATTTGAGATAGAGAGTTTCACTGGACAAGAACGTTTGGTTAATGAAGATGATTTGTCGAGTTTGAGTTATGTGCAAGGAATAATTTTGGAGACTCTGCGGTTGACCCCAGCAGCTCCTATGCTTGTGCCGCACTGTGCATCTGAAGATTGCAAAATAGAAGGCTATGATGTACCACGTGACACGATTGTGTTTGTTAACGCTTGGGCTATACATAGAGATCCAAACCTATGGGAAGATGTCACTAGTTTTAAGCCTGAAAGGCATGAAACTGCAAATGAGTCGTTTGATTCATACAAACTGTTACCATTCGGGTTGGGGAGGAGGGCATGCCCTGGTGTAGGAATGGCGCAACGTGTGGTTGGCTTGACCTTGGCGTCATTGATTCAATGTTTTGATTGGGAAAGAATGAGTAGTTCATTGGTTGACATGACTGAAGGACAAGGGATCACTATGCCGAAAGCTCAGCCATTGGTAGCCAAGTGTAAACCACGCCCTATTATGAGAGCTCTTGTTAGTGAAATAATTCACCCTTTTTAA

Coding sequence (CDS)

ATGGATAATAATTTGCTTTACATTTTCCTCTGCCTGATCTCCCTCCTTTTTGCCCTCAAATTTTTGCTGCGCCATGGCAGAAACCTCCCACCAAGCCCACTCTTTTCCCTTCCATTAATAGGCCATTTCCATCTGCTAAGACATCCCATCCACCGCAATCTTCACGAGCTCTCAAAGAAGCATGGCCACGTCTTCTCTCTCTGGTTTGGTTCTCGTCTCGCTGTAGTTGTATCATCTCCTTCCGCCGTTCGTGAATGCTTCACAAAGAACGACATCGTTCTCGCAAATCGCCCATTTCTCAACACTGGAAAGCACTTGGCCTACAACTTTACCGTACTCGCCGTCGCCCCTTACGGCGAGCTTTGGCGTAACCTTCGCCGCATCAGTACTCACGAGATTTTCTCTGCAAGCCGACTTAATTTGTTCTCCGGAATCCGACAAGATGAAGTCAAGCGCTTGTTGTGTAAATTATGCGGCGATTCGGTGGAAGAATTCTCTGTCGTCGAGCTTGAGCCGATGCTTTTGGATCTTACGTCTAACATCGTCATGAGGATGGTCGCCGGAAAGAGGTATTTTGGAGACGACGTGTCGGATGAAGGAAAAGCGAGGCAATTTAGAGATGTTGTGAAGCGAGTTATGTTGTATGCTGGGGTGACAAATCCAGGAGACTTTATACCCATATGGAATTGGATTGATCCAACTGGTCTGGAGAAGAAGATAATGAAGATTGGACAGGAAGCTGATGCAATCTTTCAGGCTTTAATCGATGAGATTCGGAGCCAAAAAGAACGTGGAAATACGATGATTGATCATTTACTTCACTTGCAGAGCTCCCAACCTGAATACTTCAATGACCAAATTATCAAAGGCCTCATACATGTTATACTACTGGCAGGGATCGATACAACGGCTGTGACATTAGAATGGGCAGTCTCCCACTTGCTCAACGAACCAGAGGTGATAAAGAAAGCGAGATTTGAGATAGAGAGTTTCACTGGACAAGAACGTTTGGTTAATGAAGATGATTTGTCGAGTTTGAGTTATGTGCAAGGAATAATTTTGGAGACTCTGCGGTTGACCCCAGCAGCTCCTATGCTTGTGCCGCACTGTGCATCTGAAGATTGCAAAATAGAAGGCTATGATGTACCACGTGACACGATTGTGTTTGTTAACGCTTGGGCTATACATAGAGATCCAAACCTATGGGAAGATGTCACTAGTTTTAAGCCTGAAAGGCATGAAACTGCAAATGAGTCGTTTGATTCATACAAACTGTTACCATTCGGGTTGGGGAGGAGGGCATGCCCTGGTGTAGGAATGGCGCAACGTGTGGTTGGCTTGACCTTGGCGTCATTGATTCAATGTTTTGATTGGGAAAGAATGAGTAGTTCATTGGTTGACATGACTGAAGGACAAGGGATCACTATGCCGAAAGCTCAGCCATTGGTAGCCAAGTGTAAACCACGCCCTATTATGAGAGCTCTTGTTAGTGAAATAATTCACCCTTTTTAA

Protein sequence

MDNNLLYIFLCLISLLFALKFLLRHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSKKHGHVFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYGELWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLCGDSVEEFSVVELEPMLLDLTSNIVMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGLEKKIMKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVILLAGIDTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILETLRLTPAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETANESFDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITMPKAQPLVAKCKPRPIMRALVSEIIHPF

Homology

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BLAST of Spg031173 vs. NCBI nr
Match: XP_038901746.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 887.1 bits (2291), Expect = 7.1e-254
Identity = 435/507 (85.80%), Postives = 468/507 (92.31%), Query Frame = 0


Query: 1   MDNNLLYIFLCLISLLFALKFLLRHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSKK 60
           MDN L+YIFL +ISLL ALK LL HG+NLPPSPLFSLPLIGH HLL+HPIH+NLH  S K
Sbjct: 1   MDNILVYIFLFVISLLLALKLLLPHGKNLPPSPLFSLPLIGHLHLLKHPIHQNLHNHSNK 60

Query: 61  HGHVFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYGE 120
           +G +FSLWFGSRL VVVSSPSAVRECFTKNDIVLANRPFLNTGKHL YNFTVLAVAPYGE
Sbjct: 61  YGRIFSLWFGSRLVVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLTYNFTVLAVAPYGE 120

Query: 121 LWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLC-GDSVEE---FSVVELEPMLLD 180
           LWR+LRRIST EIFS SRLNLFSGIRQDEVKRLLCKLC G+SVEE   FSVVE+EPMLLD
Sbjct: 121 LWRSLRRISTCEIFSTSRLNLFSGIRQDEVKRLLCKLCSGNSVEELLLFSVVEVEPMLLD 180

Query: 181 LTSNIVMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGL 240
            TSNIVMRMVAGKRYFGDDV +EG+A +FRDVVKRVM+YAG TNPGDFIP+WNWIDPTGL
Sbjct: 181 FTSNIVMRMVAGKRYFGDDVLNEGQAGKFRDVVKRVMMYAGATNPGDFIPLWNWIDPTGL 240

Query: 241 EKKIMKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVIL 300
           EKKI+K+GQEADAIFQ LIDEIR+Q E GNTMI HLLHLQ+++PEYF+DQIIKGLIHVIL
Sbjct: 241 EKKIIKVGQEADAIFQGLIDEIRTQ-EGGNTMIHHLLHLQTTRPEYFSDQIIKGLIHVIL 300

Query: 301 LAGIDTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILET 360
           LAGIDTTAVTLEWA+SHL N PEV+KKAR EIE  TGQERLVNE+DLSSLSY+QGI+LET
Sbjct: 301 LAGIDTTAVTLEWALSHLFNNPEVMKKARLEIECCTGQERLVNEEDLSSLSYLQGIVLET 360

Query: 361 LRLTPAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETA 420
           LRLTPAAP+LVPHCASEDC+IEGYD+PRDTI+FVNAWAIHRDPNLWED TSFKPERHE A
Sbjct: 361 LRLTPAAPLLVPHCASEDCQIEGYDIPRDTIIFVNAWAIHRDPNLWEDATSFKPERHENA 420

Query: 421 NESFDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITM 480
            E FDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWE M  SLVDMTEG GITM
Sbjct: 421 IELFDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWETMDGSLVDMTEGHGITM 480

Query: 481 PKAQPLVAKCKPRPIMRALVSEIIHPF 504
           PKAQPLVAKCKPRPIMRALV+E+IHPF
Sbjct: 481 PKAQPLVAKCKPRPIMRALVNEVIHPF 506

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BLAST of Spg031173 vs. NCBI nr
Match: XP_022985606.1 (cytochrome P450 81D1-like [Cucurbita maxima])

HSP 1 Score: 865.9 bits (2236), Expect = 1.7e-247
Identity = 424/503 (84.29%), Postives = 459/503 (91.25%), Query Frame = 0


Query: 1   MDNNLLYIFLCLISLLFALKFLLRHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSKK 60
           M + LLYIFL  I+LL ALKF  RHG+NLPP+PLFSLP+IGH HLL+HPIHR LH+LS K
Sbjct: 1   MAHILLYIFLFPITLLLALKFFPRHGKNLPPTPLFSLPIIGHLHLLKHPIHRTLHQLSNK 60

Query: 61  HGHVFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYGE 120
           HGHVFSL FGSRL VVVSSPSAV ECFT NDIVLANRPFLNTGKHL YNFTVLAVAPYGE
Sbjct: 61  HGHVFSLRFGSRLVVVVSSPSAVHECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPYGE 120

Query: 121 LWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLC-GDSVEEFSVVELEPMLLDLTS 180
           LWR LRRIST EIFS  RL+LF GIRQ+EVKRLLCKLC G++V    VVE++PM++DLTS
Sbjct: 121 LWRTLRRISTREIFSTCRLDLFLGIRQEEVKRLLCKLCRGNTV----VVEVQPMIMDLTS 180

Query: 181 NIVMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGLEKK 240
           NIVMRMVAGKRYFGDDVSDE +A +FRDVVKRVMLYAG TNPGDFIP+WNWIDP+G EKK
Sbjct: 181 NIVMRMVAGKRYFGDDVSDEEQAERFRDVVKRVMLYAGATNPGDFIPLWNWIDPSGFEKK 240

Query: 241 IMKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVILLAG 300
           IMK+G+EADAIFQ LIDEIR+QK  GNTMIDHLLHLQ++Q EYF+DQIIKGLIHVILLAG
Sbjct: 241 IMKVGEEADAIFQGLIDEIRNQKGSGNTMIDHLLHLQTTQSEYFSDQIIKGLIHVILLAG 300

Query: 301 IDTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILETLRL 360
           IDTTAVTLEWA+SHLLN PEVIKKARFEIE  TGQ+RLVNE+DLSSLSYVQGIILETLRL
Sbjct: 301 IDTTAVTLEWALSHLLNNPEVIKKARFEIERCTGQDRLVNEEDLSSLSYVQGIILETLRL 360

Query: 361 TPAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETANES 420
           TPAAP+LVPHCAS DCKI+GYDVPRDTI+FVNAWAIHRDPN WEDVTSF+PERHE A E 
Sbjct: 361 TPAAPLLVPHCASTDCKIQGYDVPRDTIIFVNAWAIHRDPNHWEDVTSFRPERHENAIEL 420

Query: 421 FDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITMPKA 480
            DSYKLLPFGLGRRACPGVGMAQR+VGLTLASLIQCFDWERM SSLVDMTEGQGITMPKA
Sbjct: 421 SDSYKLLPFGLGRRACPGVGMAQRIVGLTLASLIQCFDWERMDSSLVDMTEGQGITMPKA 480

Query: 481 QPLVAKCKPRPIMRALVSEIIHP 503
           QPLVAKC+PRPIMRAL+SE IHP
Sbjct: 481 QPLVAKCRPRPIMRALISEAIHP 499

[Close]

BLAST of Spg031173 vs. NCBI nr
Match: XP_023512110.1 (cytochrome P450 81D1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 862.4 bits (2227), Expect = 1.9e-246
Identity = 423/503 (84.10%), Postives = 456/503 (90.66%), Query Frame = 0


Query: 1   MDNNLLYIFLCLISLLFALKFLLRHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSKK 60
           M + LLYIFL  I+LL ALKF  RH +NLPP+PLFSLP+IGH HLL+HPIHR LH+LS K
Sbjct: 1   MAHILLYIFLFPITLLLALKFFPRHAKNLPPTPLFSLPIIGHLHLLKHPIHRTLHQLSNK 60

Query: 61  HGHVFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYGE 120
           HGHVFSL FGSRL VV+SSPSAVRECFT NDIVLANRPFLNTGKHL YNFTVLAVAPYGE
Sbjct: 61  HGHVFSLRFGSRLVVVISSPSAVRECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPYGE 120

Query: 121 LWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLC-GDSVEEFSVVELEPMLLDLTS 180
           LWR LRRIST EIFS  RL+L SGIRQDEVKRLLCKLC GD+V    VVE++PM++DLTS
Sbjct: 121 LWRTLRRISTREIFSTCRLDLSSGIRQDEVKRLLCKLCRGDTV----VVEVQPMIMDLTS 180

Query: 181 NIVMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGLEKK 240
           NIVMRMVAGKRYFGDDVSDE +A +FRDVVKRVMLYAG TNPGDFIP+WNWIDP+G EKK
Sbjct: 181 NIVMRMVAGKRYFGDDVSDEEQAERFRDVVKRVMLYAGATNPGDFIPLWNWIDPSGFEKK 240

Query: 241 IMKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVILLAG 300
           IMK+G+EADAIF  LIDEIR+QK  GNTMIDHLLHLQ++Q EYF+DQIIKGLIHVILLAG
Sbjct: 241 IMKVGEEADAIFHGLIDEIRNQKGSGNTMIDHLLHLQTTQSEYFSDQIIKGLIHVILLAG 300

Query: 301 IDTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILETLRL 360
           IDTTAVTLEWA+SHLLN PEVIKKARFEIE  TGQ+RLVNE+DLSSLSY+QGIILETLRL
Sbjct: 301 IDTTAVTLEWALSHLLNNPEVIKKARFEIERCTGQDRLVNEEDLSSLSYMQGIILETLRL 360

Query: 361 TPAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETANES 420
           TPAAP+LVPHCAS DCKI+GYDVPRDTIVFVNAWAIHRDPN WEDVTSFKPERHE A E 
Sbjct: 361 TPAAPLLVPHCASTDCKIQGYDVPRDTIVFVNAWAIHRDPNHWEDVTSFKPERHENAIEL 420

Query: 421 FDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITMPKA 480
            DSYKLLPFGLGRRACPGVGMA R++GLTLASLIQCFDWERM SS VDMTEGQGITMPKA
Sbjct: 421 SDSYKLLPFGLGRRACPGVGMAHRIIGLTLASLIQCFDWERMDSSPVDMTEGQGITMPKA 480

Query: 481 QPLVAKCKPRPIMRALVSEIIHP 503
           QPLVAKCKPRPIMRAL+SE IHP
Sbjct: 481 QPLVAKCKPRPIMRALISEAIHP 499

[Close]

BLAST of Spg031173 vs. NCBI nr
Match: XP_022943756.1 (cytochrome P450 81D1-like [Cucurbita moschata])

HSP 1 Score: 861.7 bits (2225), Expect = 3.2e-246
Identity = 422/502 (84.06%), Postives = 455/502 (90.64%), Query Frame = 0


Query: 1   MDNNLLYIFLCLISLLFALKFLLRHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSKK 60
           M + LLYIFL  I+ L ALKF  RH +NLPP+PLFSLP+IGH HLL+HPIHR LH LS K
Sbjct: 1   MAHILLYIFLFPITFLLALKFFPRHVKNLPPTPLFSLPIIGHLHLLKHPIHRTLHHLSNK 60

Query: 61  HGHVFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYGE 120
           HGHVFSL FGSRL VV+SSPSAVRECFT NDIVLANRPFLNTGKHL YNFTVLAVAPYGE
Sbjct: 61  HGHVFSLRFGSRLVVVISSPSAVRECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPYGE 120

Query: 121 LWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLCGDSVEEFSVVELEPMLLDLTSN 180
           LWR LRRIST EIFS +RL+L SGIRQDEVKRLLCKLC D   +  VVE++PM++DLTSN
Sbjct: 121 LWRTLRRISTREIFSTTRLDLSSGIRQDEVKRLLCKLCRD---DNVVVEVQPMIMDLTSN 180

Query: 181 IVMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGLEKKI 240
           IVMRMVAGKRYFGDDVSDE +A +FRDVVKRVMLYAG TNPGDFIP+WNWIDP+G EKKI
Sbjct: 181 IVMRMVAGKRYFGDDVSDEEQAERFRDVVKRVMLYAGATNPGDFIPLWNWIDPSGFEKKI 240

Query: 241 MKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVILLAGI 300
           MK+G+EADAIF  LIDEIR+QK  GNTMIDHLLHLQ++Q EYF+DQIIKGLIHVILLAGI
Sbjct: 241 MKVGEEADAIFNGLIDEIRNQKGSGNTMIDHLLHLQATQSEYFSDQIIKGLIHVILLAGI 300

Query: 301 DTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILETLRLT 360
           DTTAVTLEWA+SHLLN+PEVIKKARFEIES TGQ+RLVNE+DLSSLSY+QGIILETLRLT
Sbjct: 301 DTTAVTLEWALSHLLNDPEVIKKARFEIESCTGQDRLVNEEDLSSLSYMQGIILETLRLT 360

Query: 361 PAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETANESF 420
           PAAP+LVPHCAS DCKI+GYDVPRDTIVFVNAWAIHRDPN WEDVTSFKPERHE A E  
Sbjct: 361 PAAPLLVPHCASTDCKIQGYDVPRDTIVFVNAWAIHRDPNHWEDVTSFKPERHENAIELS 420

Query: 421 DSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITMPKAQ 480
           DSYKLLPFGLGRRACPGVGMAQR+VGLTLASLIQCFDWERM SS VDMTEGQGITMPKAQ
Sbjct: 421 DSYKLLPFGLGRRACPGVGMAQRIVGLTLASLIQCFDWERMDSSPVDMTEGQGITMPKAQ 480

Query: 481 PLVAKCKPRPIMRALVSEIIHP 503
           PLVAKCKPRPI RAL+SE IHP
Sbjct: 481 PLVAKCKPRPITRALISEAIHP 499

[Close]

BLAST of Spg031173 vs. NCBI nr
Match: XP_011650030.1 (cytochrome P450 81Q32 [Cucumis sativus] >KGN63296.1 hypothetical protein Csa_022604 [Cucumis sativus])

HSP 1 Score: 846.7 bits (2186), Expect = 1.1e-241
Identity = 418/502 (83.27%), Postives = 452/502 (90.04%), Query Frame = 0


Query: 1   MDNNLL-YIFLCLISLLFALKFLLRHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSK 60
           MDNN+L YIF  LISLL  LK LL HG+NLPP+PLFSLPLIGH HLL+HPIH+ LH LSK
Sbjct: 1   MDNNILVYIFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSK 60

Query: 61  KHGHVFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYG 120
           K+GHVFSL FGS L VV+SSPSAVRECFTKNDI+LANRPFLNTGKHL+YNFTVLAVAPYG
Sbjct: 61  KYGHVFSLRFGSHLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYG 120

Query: 121 ELWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLCG-DSVEE---FSVVELEPMLL 180
           ELWR LRRIST EIFS S+LN FS IRQDEVKRLL KLC  +SVEE   FS VELEP+LL
Sbjct: 121 ELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILL 180

Query: 181 DLTSNIVMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTG 240
           D TSNIVMRMV GKRYFGDDV DEG+A +FRDVVKRVMLYAG TNPGDFIP+WNWIDPTG
Sbjct: 181 DFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTG 240

Query: 241 LEKKIMKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVI 300
           LEKKIMK+G+EAD IFQ LIDEIR+++E GNTMI HLLHLQ++QPEYF+DQIIKGLIHVI
Sbjct: 241 LEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVI 300

Query: 301 LLAGIDTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILE 360
            LAGIDT +VTLEW +SHLLN P+VIKKAR EIE   GQERLVNEDDLSSLSY+QGIILE
Sbjct: 301 FLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILE 360

Query: 361 TLRLTPAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHET 420
           TLRLTPAAP+LVPHCASEDC+IEGYD+PR TI+FVNAWAIHRD +LWEDVTSFKPERHE 
Sbjct: 361 TLRLTPAAPLLVPHCASEDCQIEGYDIPRGTIIFVNAWAIHRDSSLWEDVTSFKPERHEN 420

Query: 421 ANESFDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGIT 480
           A E  DSYKLLPFGLGRRACPGVGMAQRV+GLTLASLIQCFDWERM SSLVDMTEGQGIT
Sbjct: 421 AIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGIT 480

Query: 481 MPKAQPLVAKCKPRPIMRALVS 498
           MPKAQPLVAKCKPRPIMRA +S
Sbjct: 481 MPKAQPLVAKCKPRPIMRAHLS 502

[Close]

BLAST of Spg031173 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 4.1e-164
Identity = 288/498 (57.83%), Postives = 362/498 (72.69%), Query Frame = 0


Query: 3   NNLLYIFLCLISLLFALKF--LLRHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSKK 62
           + LLY FL ++ L  +LK   + R  RNLPPSP  +LP+IGH HL+   +HR+L++LSKK
Sbjct: 4   STLLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKK 63

Query: 63  HGHVFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYGE 122
           +G VFSL  G+RL +VVSSP+A  ECFTKNDIV ANRP    GK++ YN+T +  +PYGE
Sbjct: 64  YGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGE 123

Query: 123 LWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLCGDSVEEFSVVELEPMLLDLTSN 182
            WRNLRR++  EIFSA  LN F  IR+DEVK+LL  L   S ++F  VE++  L +L+ N
Sbjct: 124 HWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFN 183

Query: 183 IVMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGLEKKI 242
           + MRMVAGKRYFG DV D  +A+ FR ++  V  +AG +NPGDF+P   WID    EKK+
Sbjct: 184 VTMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKV 243

Query: 243 MKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVILLAGI 302
            KI QE DA  Q LI E R  K    TMIDHLL LQ SQPEY+ DQIIKG+I V+LLAG 
Sbjct: 244 SKISQEMDAFLQRLIHESRINK-NNVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGT 303

Query: 303 DTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILETLRLT 362
           DT+AVT+EWA+S LLN PE ++KAR EIE+  G  RL+ E DL  L+Y+  II ET RL 
Sbjct: 304 DTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLC 363

Query: 363 PAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETANESF 422
           PAAPMLVPH +S+DCK++GYDVP+ TI+ VNAWAIHRDP  W++ T FKPERH       
Sbjct: 364 PAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERH--GGVEL 423

Query: 423 DSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITMPKAQ 482
           +  KL+PFG+GRR+CPG G+AQRVVGLTL +LIQCF+W+R+  + +DM EG G+TMPKAQ
Sbjct: 424 EPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQ 483

Query: 483 PLVAKCKPRPIMRALVSE 499
           PL A CKPR I+  +VSE
Sbjct: 484 PLEALCKPRNILHKVVSE 497

[Close]

BLAST of Spg031173 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 527.3 bits (1357), Expect = 1.9e-148
Identity = 255/493 (51.72%), Postives = 347/493 (70.39%), Query Frame = 0


Query: 8   IFLCLISLLFALKFLLRHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSKKHGHVFSL 67
           +F   +  +F +    R  +NLPP P  SLP+IG+ H L+ P+HR    LS+K+GHVFSL
Sbjct: 11  VFYLALFFIFNIVIRARKFKNLPPGPP-SLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSL 70

Query: 68  WFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYGELWRNLRR 127
           WFGSRL VVVSS S  ++CFTKND+VLANRP   +GK++ YN+T L    YGE WRNLRR
Sbjct: 71  WFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRR 130

Query: 128 ISTHEIFSASRLNLFSGIRQDEVKRLLCKLCGDSVEEFSVVELEPMLLDLTSNIVMRMVA 187
           I+  ++ S  R+N FSGIR+DE +RL+ +L  DS   F+ +EL   L D+T N +MRM++
Sbjct: 131 ITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMIS 190

Query: 188 GKRYFGD--DVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGLEKKIMKIGQ 247
           GKRY+G+  D SD  +A QFRD+V  ++  +G  N  DF+P+  ++D   LEK++  I  
Sbjct: 191 GKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISG 250

Query: 248 EADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVILLAGIDTTAV 307
           + DA  + LI+E R++KER NTMIDHLL+LQ SQPEY+ DQIIKGL   +LLAG D++AV
Sbjct: 251 KTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAV 310

Query: 308 TLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILETLRLTPAAPM 367
           TLEW++S+LLN PEV+KK + E+++  GQ+RLV+E DL  L+Y++ +I ETLRL   AP+
Sbjct: 311 TLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPL 370

Query: 368 LVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETANESFDSYKL 427
           L+PH  S++C I GY VP+DTIV +NAWAIHRDP LW + T+FKPER E   E     KL
Sbjct: 371 LLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGE---LEKL 430

Query: 428 LPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITMPKAQPLVAK 487
           + FG+GRRACPG G+A R + +TLA LIQCFDW+ ++   +D+ E  G T+ K  PL A 
Sbjct: 431 IAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLVPLKAM 490

Query: 488 CKPRPIMRALVSE 499
           CK RP++  +  +
Sbjct: 491 CKSRPVINKVFKQ 499

[Close]

BLAST of Spg031173 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 9.3e-148
Identity = 266/489 (54.40%), Postives = 338/489 (69.12%), Query Frame = 0


Query: 6   LYIFLCLISLLFALKFLL---RHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSKKHG 65
           L + + L  L+  LK      R  +NLPP P   LP+IG+ H L+ P+H   H LS+K+G
Sbjct: 6   LSLIISLFFLIITLKVFFNTSRKFKNLPPGPQ-CLPIIGNLHQLKQPLHHTFHTLSQKYG 65

Query: 66  HVFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYGELW 125
            +FSLWFGSRL VVVSS +  +ECFTKNDIVLANRP   TGK++ YN T +A +PYG+ W
Sbjct: 66  QIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHW 125

Query: 126 RNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLCGDSVEEFSVVELEPMLLDLTSNIV 185
           RNLRRI + EI S+ RLN F  IR+DE+ RL+ KL   S   F+ VEL PM  ++T N +
Sbjct: 126 RNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTI 185

Query: 186 MRMVAGKRYFGD--DVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGLEKKI 245
           MRMV+GKRY+G+  DVSD  +AR FR ++K V+   G  N GDF+    W D  GLEK++
Sbjct: 186 MRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRL 245

Query: 246 MKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVILLAGI 305
            KI +  DA  Q LIDE R  K   NTMIDHLL  Q SQPEY+ DQIIKGL+ V+LLAG 
Sbjct: 246 KKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGT 305

Query: 306 DTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILETLRLT 365
           DT++VT+EWA+S+LLN PE++KKA+ E+++  G +R V+E D+S L Y+Q I+ ETLRL 
Sbjct: 306 DTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLH 365

Query: 366 PAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETANESF 425
            AAP+LVPH +SED  + GY++P++TI+ VNAW IHRDPNLW D T FKPER E   E  
Sbjct: 366 AAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKEGE-- 425

Query: 426 DSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITMPKAQ 485
              KLL FGLGRRACPG  ++QR  GLTL  LIQCF+W+R+    +DM E +GIT  K  
Sbjct: 426 -VNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAGKKT 485

Query: 486 PLVAKCKPR 490
            L A CK R
Sbjct: 486 SLNAMCKVR 490

[Close]

BLAST of Spg031173 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.3e-146
Identity = 264/498 (53.01%), Postives = 344/498 (69.08%), Query Frame = 0


Query: 5   LLYIFLCLISLLFALKFLL----RHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSKK 64
           L Y  L  +S +  +K LL    R  +NLPP P  ++P+IG+ H L+HP+HR    LS+ 
Sbjct: 2   LFYYSLLSLSFIITIKILLKITSRRLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQT 61

Query: 65  HGHVFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYGE 124
           +G +FSLWFGSRL VVVSSPS   ECFTKNDI+LANRP   TGK++ YN+T L  A YG+
Sbjct: 62  YGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGD 121

Query: 125 LWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLCGDSVEE---FSVVELEPMLLDL 184
            WRNLRRI+T ++ S +RLN F G+R+DE  RL+ KL  D V E   F+ VEL P L ++
Sbjct: 122 HWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEM 181

Query: 185 TSNIVMRMVAGKRYFGD--DVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTG 244
           T N +MRM++GKRY+GD  DVSD  +A+QFR+++  +M   G  N GDF+P+   +D   
Sbjct: 182 TFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDN 241

Query: 245 LEKKIMKIGQEADAIFQALIDEIR--SQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIH 304
           LEK+  +I + ++A  + LI+E R  +    G TMIDHLL L  SQPEY++D +IKGLI 
Sbjct: 242 LEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQ 301

Query: 305 VILLAGIDTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGII 364
            +LLAG DT+AVT+EW +S LLN PEV+KKA+ E+++  G+ +LV+E DLS L Y+Q II
Sbjct: 302 GMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNII 361

Query: 365 LETLRLTPAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERH 424
            ETLRL P AP+L+PH +SEDC I  ++VP+DTI+  N W IHRDP  W D  SFKPER 
Sbjct: 362 SETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERF 421

Query: 425 ETANESFDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQG 484
           E   E     K++ FGLGRRACPG+ +AQR VG T+  LIQCF+WER S   +DM EG+G
Sbjct: 422 EKEEE---VNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKG 481

Query: 485 ITMPKAQPLVAKCKPRPI 492
           ITMP   PL A CK  PI
Sbjct: 482 ITMPMKIPLRAMCKALPI 495

[Close]

BLAST of Spg031173 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 8.7e-146
Identity = 271/499 (54.31%), Postives = 344/499 (68.94%), Query Frame = 0


Query: 5   LLYIFLCLISLLFALKFLLRHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSKKHGH- 64
           +LY    LI L+ + KFL    +NLPPSP   LP+IGH  LL+ PIHR L   S+   H 
Sbjct: 9   VLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHN 68

Query: 65  ----VFSLWFGSRLAVVVSSPS-AVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPY 124
               V SL  GSRL  VVSS   A  ECF KND+VLANRP +  GKH+ YN T +  APY
Sbjct: 69  DGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPY 128

Query: 125 GELWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLCGDSVEEFSVVELEPMLLDLT 184
           G+ WRNLRR+ T EIFS  RLN F  +R DEV+RL+ +L   +  + +VVEL+PML+DLT
Sbjct: 129 GDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLT 188

Query: 185 SNIVMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGLEK 244
            N +MRM+ GKRY+G++ +DE +A++ R +V  V       N  D++PI      +  E 
Sbjct: 189 FNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SSYEN 248

Query: 245 KIMKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVILLA 304
           ++ K+G+E D   Q LID+ R Q+E G TMIDHLL LQ S  EY+ DQIIKG+I ++++A
Sbjct: 249 RVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIMVIA 308

Query: 305 GIDTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILETLR 364
           G +T+AVTLEWA+S+LLN P+VI KAR EI++  G +RL+ E DLS L Y++ I+LETLR
Sbjct: 309 GTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLR 368

Query: 365 LTPAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETANE 424
           L PA P+LVPH ASEDCKI  YD+PR T + VNAWAIHRDPN W+D  SFKPER E   E
Sbjct: 369 LHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKEEE 428

Query: 425 SFDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITMPK 484
              + KLL FGLGRRACPG G+AQR+VGL L SLIQCF+WER+ +  VDM EG G T+PK
Sbjct: 429 ---AQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPK 488

Query: 485 AQPLVAKCKPRPIMRALVS 498
           A PL A CK RP +  ++S
Sbjct: 489 AIPLKAICKARPFLHKIIS 502

[Close]

BLAST of Spg031173 vs. ExPASy TrEMBL
Match: A0A6J1J8Q1 (cytochrome P450 81D1-like OS=Cucurbita maxima OX=3661 GN=LOC111483619 PE=3 SV=1)

HSP 1 Score: 865.9 bits (2236), Expect = 8.2e-248
Identity = 424/503 (84.29%), Postives = 459/503 (91.25%), Query Frame = 0


Query: 1   MDNNLLYIFLCLISLLFALKFLLRHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSKK 60
           M + LLYIFL  I+LL ALKF  RHG+NLPP+PLFSLP+IGH HLL+HPIHR LH+LS K
Sbjct: 1   MAHILLYIFLFPITLLLALKFFPRHGKNLPPTPLFSLPIIGHLHLLKHPIHRTLHQLSNK 60

Query: 61  HGHVFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYGE 120
           HGHVFSL FGSRL VVVSSPSAV ECFT NDIVLANRPFLNTGKHL YNFTVLAVAPYGE
Sbjct: 61  HGHVFSLRFGSRLVVVVSSPSAVHECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPYGE 120

Query: 121 LWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLC-GDSVEEFSVVELEPMLLDLTS 180
           LWR LRRIST EIFS  RL+LF GIRQ+EVKRLLCKLC G++V    VVE++PM++DLTS
Sbjct: 121 LWRTLRRISTREIFSTCRLDLFLGIRQEEVKRLLCKLCRGNTV----VVEVQPMIMDLTS 180

Query: 181 NIVMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGLEKK 240
           NIVMRMVAGKRYFGDDVSDE +A +FRDVVKRVMLYAG TNPGDFIP+WNWIDP+G EKK
Sbjct: 181 NIVMRMVAGKRYFGDDVSDEEQAERFRDVVKRVMLYAGATNPGDFIPLWNWIDPSGFEKK 240

Query: 241 IMKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVILLAG 300
           IMK+G+EADAIFQ LIDEIR+QK  GNTMIDHLLHLQ++Q EYF+DQIIKGLIHVILLAG
Sbjct: 241 IMKVGEEADAIFQGLIDEIRNQKGSGNTMIDHLLHLQTTQSEYFSDQIIKGLIHVILLAG 300

Query: 301 IDTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILETLRL 360
           IDTTAVTLEWA+SHLLN PEVIKKARFEIE  TGQ+RLVNE+DLSSLSYVQGIILETLRL
Sbjct: 301 IDTTAVTLEWALSHLLNNPEVIKKARFEIERCTGQDRLVNEEDLSSLSYVQGIILETLRL 360

Query: 361 TPAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETANES 420
           TPAAP+LVPHCAS DCKI+GYDVPRDTI+FVNAWAIHRDPN WEDVTSF+PERHE A E 
Sbjct: 361 TPAAPLLVPHCASTDCKIQGYDVPRDTIIFVNAWAIHRDPNHWEDVTSFRPERHENAIEL 420

Query: 421 FDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITMPKA 480
            DSYKLLPFGLGRRACPGVGMAQR+VGLTLASLIQCFDWERM SSLVDMTEGQGITMPKA
Sbjct: 421 SDSYKLLPFGLGRRACPGVGMAQRIVGLTLASLIQCFDWERMDSSLVDMTEGQGITMPKA 480

Query: 481 QPLVAKCKPRPIMRALVSEIIHP 503
           QPLVAKC+PRPIMRAL+SE IHP
Sbjct: 481 QPLVAKCRPRPIMRALISEAIHP 499

[Close]

BLAST of Spg031173 vs. ExPASy TrEMBL
Match: A0A6J1FSK8 (cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448412 PE=3 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 1.5e-246
Identity = 422/502 (84.06%), Postives = 455/502 (90.64%), Query Frame = 0


Query: 1   MDNNLLYIFLCLISLLFALKFLLRHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSKK 60
           M + LLYIFL  I+ L ALKF  RH +NLPP+PLFSLP+IGH HLL+HPIHR LH LS K
Sbjct: 1   MAHILLYIFLFPITFLLALKFFPRHVKNLPPTPLFSLPIIGHLHLLKHPIHRTLHHLSNK 60

Query: 61  HGHVFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYGE 120
           HGHVFSL FGSRL VV+SSPSAVRECFT NDIVLANRPFLNTGKHL YNFTVLAVAPYGE
Sbjct: 61  HGHVFSLRFGSRLVVVISSPSAVRECFTNNDIVLANRPFLNTGKHLTYNFTVLAVAPYGE 120

Query: 121 LWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLCGDSVEEFSVVELEPMLLDLTSN 180
           LWR LRRIST EIFS +RL+L SGIRQDEVKRLLCKLC D   +  VVE++PM++DLTSN
Sbjct: 121 LWRTLRRISTREIFSTTRLDLSSGIRQDEVKRLLCKLCRD---DNVVVEVQPMIMDLTSN 180

Query: 181 IVMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGLEKKI 240
           IVMRMVAGKRYFGDDVSDE +A +FRDVVKRVMLYAG TNPGDFIP+WNWIDP+G EKKI
Sbjct: 181 IVMRMVAGKRYFGDDVSDEEQAERFRDVVKRVMLYAGATNPGDFIPLWNWIDPSGFEKKI 240

Query: 241 MKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVILLAGI 300
           MK+G+EADAIF  LIDEIR+QK  GNTMIDHLLHLQ++Q EYF+DQIIKGLIHVILLAGI
Sbjct: 241 MKVGEEADAIFNGLIDEIRNQKGSGNTMIDHLLHLQATQSEYFSDQIIKGLIHVILLAGI 300

Query: 301 DTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILETLRLT 360
           DTTAVTLEWA+SHLLN+PEVIKKARFEIES TGQ+RLVNE+DLSSLSY+QGIILETLRLT
Sbjct: 301 DTTAVTLEWALSHLLNDPEVIKKARFEIESCTGQDRLVNEEDLSSLSYMQGIILETLRLT 360

Query: 361 PAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETANESF 420
           PAAP+LVPHCAS DCKI+GYDVPRDTIVFVNAWAIHRDPN WEDVTSFKPERHE A E  
Sbjct: 361 PAAPLLVPHCASTDCKIQGYDVPRDTIVFVNAWAIHRDPNHWEDVTSFKPERHENAIELS 420

Query: 421 DSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITMPKAQ 480
           DSYKLLPFGLGRRACPGVGMAQR+VGLTLASLIQCFDWERM SS VDMTEGQGITMPKAQ
Sbjct: 421 DSYKLLPFGLGRRACPGVGMAQRIVGLTLASLIQCFDWERMDSSPVDMTEGQGITMPKAQ 480

Query: 481 PLVAKCKPRPIMRALVSEIIHP 503
           PLVAKCKPRPI RAL+SE IHP
Sbjct: 481 PLVAKCKPRPITRALISEAIHP 499

[Close]

BLAST of Spg031173 vs. ExPASy TrEMBL
Match: A0A0A0LTD0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425740 PE=3 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 5.1e-242
Identity = 418/502 (83.27%), Postives = 452/502 (90.04%), Query Frame = 0


Query: 1   MDNNLL-YIFLCLISLLFALKFLLRHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSK 60
           MDNN+L YIF  LISLL  LK LL HG+NLPP+PLFSLPLIGH HLL+HPIH+ LH LSK
Sbjct: 1   MDNNILVYIFFFLISLLLGLKLLLHHGKNLPPTPLFSLPLIGHLHLLKHPIHQTLHSLSK 60

Query: 61  KHGHVFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYG 120
           K+GHVFSL FGS L VV+SSPSAVRECFTKNDI+LANRPFLNTGKHL+YNFTVLAVAPYG
Sbjct: 61  KYGHVFSLRFGSHLVVVISSPSAVRECFTKNDIILANRPFLNTGKHLSYNFTVLAVAPYG 120

Query: 121 ELWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLCG-DSVEE---FSVVELEPMLL 180
           ELWR LRRIST EIFS S+LN FS IRQDEVKRLL KLC  +SVEE   FS VELEP+LL
Sbjct: 121 ELWRRLRRISTCEIFSTSKLNSFSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILL 180

Query: 181 DLTSNIVMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTG 240
           D TSNIVMRMV GKRYFGDDV DEG+A +FRDVVKRVMLYAG TNPGDFIP+WNWIDPTG
Sbjct: 181 DFTSNIVMRMVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTG 240

Query: 241 LEKKIMKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVI 300
           LEKKIMK+G+EAD IFQ LIDEIR+++E GNTMI HLLHLQ++QPEYF+DQIIKGLIHVI
Sbjct: 241 LEKKIMKVGEEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVI 300

Query: 301 LLAGIDTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILE 360
            LAGIDT +VTLEW +SHLLN P+VIKKAR EIE   GQERLVNEDDLSSLSY+QGIILE
Sbjct: 301 FLAGIDTISVTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILE 360

Query: 361 TLRLTPAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHET 420
           TLRLTPAAP+LVPHCASEDC+IEGYD+PR TI+FVNAWAIHRD +LWEDVTSFKPERHE 
Sbjct: 361 TLRLTPAAPLLVPHCASEDCQIEGYDIPRGTIIFVNAWAIHRDSSLWEDVTSFKPERHEN 420

Query: 421 ANESFDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGIT 480
           A E  DSYKLLPFGLGRRACPGVGMAQRV+GLTLASLIQCFDWERM SSLVDMTEGQGIT
Sbjct: 421 AIELSDSYKLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGIT 480

Query: 481 MPKAQPLVAKCKPRPIMRALVS 498
           MPKAQPLVAKCKPRPIMRA +S
Sbjct: 481 MPKAQPLVAKCKPRPIMRAHLS 502

[Close]

BLAST of Spg031173 vs. ExPASy TrEMBL
Match: A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 1.1e-212
Identity = 357/497 (71.83%), Postives = 423/497 (85.11%), Query Frame = 0


Query: 1   MDNNLLYIFLCLISLLFALKFLLR-HGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSK 60
           MD  LL I L L SL     FLLR + RNLPPSP F LPLIGH HL++HP+HR LH+LS 
Sbjct: 81  MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140

Query: 61  KHGHVFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYG 120
            +GHVFSL +GSRL VVVSSPSAV+ECFTKNDIVLANRP L +GK LAYN T LAV+PYG
Sbjct: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200

Query: 121 ELWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLCGDSVEEFSVVELEPMLLDLTS 180
           E WRNLRRIST EIFS SRLNLF GIR+DEVK LL KL GDS++EF  VEL+ MLLD T 
Sbjct: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260

Query: 181 NIVMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGLEKK 240
           NIV+RM+AGK+Y+G+D+ DEG++++FR++VK ++   G  N GDF+P+WNWIDPTG EK+
Sbjct: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320

Query: 241 IMKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVILLAG 300
           IMKIG+ +D + Q L+DEIR+QK+ GNTMIDHLL LQ++QPEYF+DQIIKGL  ++LLAG
Sbjct: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380

Query: 301 IDTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILETLRL 360
           IDT A+TLEWA+S LLN PEV+KKAR EI+ F G++RLV+E DLSSLSY+QG+I ETLRL
Sbjct: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440

Query: 361 TPAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETANES 420
            PAAP+LVPHCASEDCKIEGYD+PRDTI+ +NAWAIHRD N+WED TSFKPERH      
Sbjct: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGG 500

Query: 421 FDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITMPKA 480
            DSYKLLPFGLGRRACPG+GMAQRVVGLTLASLIQCF+WER++SSLVDMTEG+G+TMPKA
Sbjct: 501 VDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKA 560

Query: 481 QPLVAKCKPRPIMRALV 497
           QPLVAKCKPRPI++A++
Sbjct: 561 QPLVAKCKPRPILKAIL 577

[Close]

BLAST of Spg031173 vs. ExPASy TrEMBL
Match: A0A6J1D5S1 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 8.5e-205
Identity = 355/501 (70.86%), Postives = 416/501 (83.03%), Query Frame = 0


Query: 1   MDNNLLYIFLCLISLLFALKFLLRHGR-NLPPSPLFSLPLIGHFHLLRHPIHRNLHELSK 60
           MD   L I L L SLL A  FL R  R NLPPSPLFSLP++GH HL++HP+H+  HELS 
Sbjct: 1   MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60

Query: 61  KHGHVFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYG 120
           K+GHVFSL FGSR+ VVVSSPSAV+ECFTKNDIVLANRP L +GK LAYN T +AVAPYG
Sbjct: 61  KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120

Query: 121 ELWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLCGDSVEEFSVVELEPMLLDLTS 180
           E WRNLRRI T EIFS +RLN+F GIR+DEV+ L+ KLC +S EEF+ VELEPML DLT 
Sbjct: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180

Query: 181 NIVMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGLEKK 240
           NIVMRMVAGK+Y+     +E ++++FR++V +V+ + G TNPGDFIPIWNWIDPTG EK+
Sbjct: 181 NIVMRMVAGKKYY-----EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKR 240

Query: 241 IMKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVILLAG 300
           I+K+GQ +D + Q LIDEIR+Q+   NTMIDHLL LQ ++PEY++DQIIKGLI VILLAG
Sbjct: 241 IIKLGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAG 300

Query: 301 IDTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILETLRL 360
           IDT AVTLEWA+S LLN PEV++KA+ EI+ F GQERLVNE DLSSL Y+QGII ETLRL
Sbjct: 301 IDTAAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRL 360

Query: 361 TPAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETANES 420
            PAAP+L+PHCAS DCKI GYDVPRDTI+ +N WAIHRDPNLWED T+FKPERH      
Sbjct: 361 NPAAPLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGN-TIG 420

Query: 421 FDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITMPKA 480
            DSYKLLPFG GRR+CPGVGMAQRVVGLTLASLIQ F+WERMS+SLVDMTEG+G+TMPKA
Sbjct: 421 VDSYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKA 480

Query: 481 QPLVAKCKPRPIMRALVSEII 501
           Q LVAKCKPRPIM+ +++E I
Sbjct: 481 QHLVAKCKPRPIMKLILNEAI 495

[Close]

BLAST of Spg031173 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 518.8 bits (1335), Expect = 4.7e-147
Identity = 261/499 (52.30%), Postives = 351/499 (70.34%), Query Frame = 0


Query: 4   NLLYIFLCLISLLFALKFLLRHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSK--KH 63
           ++L++ L LI L+  LK       NLPPSP +SLP+IGH  LL+ PIHR    LS+   +
Sbjct: 9   SILFVVLSLIYLIGKLK----RKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNN 68

Query: 64  GHVFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYGEL 123
             +FSL  G+RL  V SS S   ECFTKND+VLANRP     KH+AY++T +  A YG+ 
Sbjct: 69  APIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDH 128

Query: 124 WRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLCGDSVEEFSVVELEPMLLDLTSNI 183
           WRNLRRI + EIFS  RLN F  IR+DE++RL+ +L  +  +EF  V+++ ML DLT N 
Sbjct: 129 WRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNN 188

Query: 184 VMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGLEKKIM 243
           ++RMVAGKRY+GD V D+ +A++ R ++  V+  AG  N  D++P+   +  +  E ++ 
Sbjct: 189 ILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVK 248

Query: 244 KIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVILLAGID 303
           K+    D   Q L+DE R  KE+GNTMIDHLL LQ SQP+YF D+IIKG +  ++LAG D
Sbjct: 249 KLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTD 308

Query: 304 TTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILETLRLTP 363
           T+AVTLEWA+S++LN P+V+ KAR EI+   G +RL++E D+S+L Y+Q I+ ETLRL P
Sbjct: 309 TSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYP 368

Query: 364 AAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETANESFD 423
           AAPML+PH ASEDCK+ GYD+PR TI+  N WAIHRDP LW+D  SFKPER E   E   
Sbjct: 369 AAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKEGE--- 428

Query: 424 SYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITMPKAQP 483
           + KL+PFGLGRRACPG G+A R++ LTL SLIQC +WE++    VDM+EG+G+TMPKA+P
Sbjct: 429 AQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAKP 488

Query: 484 LVAKCKPRPIMRALVSEII 501
           L A C+ RP +  + +E +
Sbjct: 489 LEAMCRARPSVVKIFNESV 497

[Close]

BLAST of Spg031173 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 518.5 bits (1334), Expect = 6.2e-147
Identity = 271/499 (54.31%), Postives = 344/499 (68.94%), Query Frame = 0


Query: 5   LLYIFLCLISLLFALKFLLRHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSKKHGH- 64
           +LY    LI L+ + KFL    +NLPPSP   LP+IGH  LL+ PIHR L   S+   H 
Sbjct: 9   VLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHN 68

Query: 65  ----VFSLWFGSRLAVVVSSPS-AVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPY 124
               V SL  GSRL  VVSS   A  ECF KND+VLANRP +  GKH+ YN T +  APY
Sbjct: 69  DGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPY 128

Query: 125 GELWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLCGDSVEEFSVVELEPMLLDLT 184
           G+ WRNLRR+ T EIFS  RLN F  +R DEV+RL+ +L   +  + +VVEL+PML+DLT
Sbjct: 129 GDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLT 188

Query: 185 SNIVMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGLEK 244
            N +MRM+ GKRY+G++ +DE +A++ R +V  V       N  D++PI      +  E 
Sbjct: 189 FNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SSYEN 248

Query: 245 KIMKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVILLA 304
           ++ K+G+E D   Q LID+ R Q+E G TMIDHLL LQ S  EY+ DQIIKG+I ++++A
Sbjct: 249 RVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIMVIA 308

Query: 305 GIDTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILETLR 364
           G +T+AVTLEWA+S+LLN P+VI KAR EI++  G +RL+ E DLS L Y++ I+LETLR
Sbjct: 309 GTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLR 368

Query: 365 LTPAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETANE 424
           L PA P+LVPH ASEDCKI  YD+PR T + VNAWAIHRDPN W+D  SFKPER E   E
Sbjct: 369 LHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKEEE 428

Query: 425 SFDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITMPK 484
              + KLL FGLGRRACPG G+AQR+VGL L SLIQCF+WER+ +  VDM EG G T+PK
Sbjct: 429 ---AQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPK 488

Query: 485 AQPLVAKCKPRPIMRALVS 498
           A PL A CK RP +  ++S
Sbjct: 489 AIPLKAICKARPFLHKIIS 502

[Close]

BLAST of Spg031173 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 514.6 bits (1324), Expect = 8.9e-146
Identity = 260/500 (52.00%), Postives = 340/500 (68.00%), Query Frame = 0


Query: 1   MDNN--LLYIFLCLISLLFALKFLLRHGRNLPPSPLFSLPLIGHFHLLRHPIHRNLHELS 60
           MD N  L+  FL L +L   L    +   NLPPSP  SLP+IGH HLL+ P+HR    LS
Sbjct: 1   MDLNQILILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLS 60

Query: 61  KKHGH--VFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVA 120
           K  G+  VF L  G+RL  V+SS S   ECFTKND+VLANRP     KHL YN T L  A
Sbjct: 61  KSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSA 120

Query: 121 PYGELWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLCGDSVEEFSVVELEPMLLD 180
            YG+ WRNLRRI+  EIFS  RLN F  IR+DE++RL+  L  DS+  F  VE++ +L +
Sbjct: 121 SYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTN 180

Query: 181 LTSNIVMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGL 240
           L SN  +RM+AGKRYFG+D  D   A+  +++V   +  AG  NP D++ I  W+  +  
Sbjct: 181 LASNTTIRMLAGKRYFGEDNDD---AKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSY 240

Query: 241 EKKIMKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVIL 300
           EK+I  +G   D   Q L+DE R++KE+G TMIDHLL LQ  QP+Y+ D IIKG+I  ++
Sbjct: 241 EKRIKNLGNRFDTFLQKLVDEKRAEKEKGETMIDHLLALQDIQPDYYTDVIIKGIILTLI 300

Query: 301 LAGIDTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILET 360
           +AG DT++VTLEWA+S+LLN PE++KKAR EI+   G +RLV+E D+ +LSY+Q I+LET
Sbjct: 301 IAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLET 360

Query: 361 LRLTPAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETA 420
           LR+ PA P+L+PH +SEDCK+ GYD+P  T+V  NAWA+HRDP +WED   FKPER E  
Sbjct: 361 LRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEKE 420

Query: 421 NESFDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITM 480
            E   + KL+ FG+GRRACPG G+A R++   L SL+QCF+WER+    VDMTE +G T+
Sbjct: 421 GE---AEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATL 480

Query: 481 PKAQPLVAKCKPRPIMRALV 497
           PKA PL A CK R I+  L+
Sbjct: 481 PKAIPLRAMCKARSIVDKLI 492

[Close]

BLAST of Spg031173 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 511.5 bits (1316), Expect = 7.5e-145
Identity = 247/490 (50.41%), Postives = 338/490 (68.98%), Query Frame = 0


Query: 8   IFLCLISLLFALKFLLRHGR---NLPPSPLFSLPLIGHFHLLRHPIHRNLHELSKKHGH- 67
           + L  + L  ++K LL       NLPPSP + LP+IGH HLL+ P+HR  H +SK  G+ 
Sbjct: 7   LLLSFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNA 66

Query: 68  -VFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPYGELW 127
            +F L  G+RL  V+SS S   ECFTKND+VLANRP +   KH+ YNFT +  A YG+ W
Sbjct: 67  PIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHW 126

Query: 128 RNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLCGDSVEEFSVVELEPMLLDLTSNIV 187
           RNLRRI+  EIFS+ R++ FS IR+DE++RL+  L  DS+  F  VEL+ +L +L  N +
Sbjct: 127 RNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNI 186

Query: 188 MRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGLEKKIMK 247
           + MVAGKRY+G    D  +A+  R+++  +M  AG  N  D++P  NW+  T  E +   
Sbjct: 187 IMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TNFENQTKI 246

Query: 248 IGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVILLAGIDT 307
           +G   D + Q L+DE R++KE+G T+IDHLL  Q ++PEY+ D IIKG+I  ++LAG DT
Sbjct: 247 LGNRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVLAGTDT 306

Query: 308 TAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILETLRLTPA 367
           ++VTLEWA+S+LLN PE+++KAR EI+   G +RLV E D+ +L Y+Q I+ ETLRL PA
Sbjct: 307 SSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLRLYPA 366

Query: 368 APMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETANESFDS 427
            P+L+PH +S++CK+ GYD+PR T++  N WA+HRDP LWE+   FKPER E   E   +
Sbjct: 367 VPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERFEKEGE---A 426

Query: 428 YKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQGITMPKAQPL 487
            KL+PFG+GRRACPG  + +R+V L L  LIQ F+WER+ + LVDMTEG+GITMPKA PL
Sbjct: 427 RKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITMPKATPL 486

Query: 488 VAKCKPRPIM 493
            A CK R I+
Sbjct: 487 RAMCKARAIV 491

[Close]

BLAST of Spg031173 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 502.3 bits (1292), Expect = 4.6e-142
Identity = 255/499 (51.10%), Postives = 334/499 (66.93%), Query Frame = 0


Query: 6   LYIFLCLISLLFALKFLLRHG-----RNLPPSPLFSLPLIGHFHLLRHPIHRNLHELSK- 65
           +Y+ L L      L   L  G      NLPPSP    P+IGH HLL+ P+HR    LS+ 
Sbjct: 11  IYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSES 70

Query: 66  -KHGHVFSLWFGSRLAVVVSSPSAVRECFTKNDIVLANRPFLNTGKHLAYNFTVLAVAPY 125
             +  +FSL  GSRL  VVSS +   ECFTKND+VLANRP    GKH+ YN T +  A Y
Sbjct: 71  LNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAY 130

Query: 126 GELWRNLRRISTHEIFSASRLNLFSGIRQDEVKRLLCKLCGDSVEEFSVVELEPMLLDLT 185
           G+ WRNLRRI T EIFS+ RLN F  IRQDE++RL+  L  +S   F  VE++P+ + LT
Sbjct: 131 GDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLT 190

Query: 186 SNIVMRMVAGKRYFGDDVSDEGKARQFRDVVKRVMLYAGVTNPGDFIPIWNWIDPTGLEK 245
            N ++RMVAGKR++GD   ++ +A+  R ++  V++  G  N  D+ PI  ++  T  EK
Sbjct: 191 INNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TNYEK 250

Query: 246 KIMKIGQEADAIFQALIDEIRSQKERGNTMIDHLLHLQSSQPEYFNDQIIKGLIHVILLA 305
            + K+    D   Q+L++E R +K +GNTMIDHLL LQ +QP+Y+ D IIKG+I V++LA
Sbjct: 251 HVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVMILA 310

Query: 306 GIDTTAVTLEWAVSHLLNEPEVIKKARFEIESFTGQERLVNEDDLSSLSYVQGIILETLR 365
           G DT+A TLEWA+S+LLN PEV++KA+ EI+   G +RLV E D+  L Y+Q I+ ETLR
Sbjct: 311 GTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLR 370

Query: 366 LTPAAPMLVPHCASEDCKIEGYDVPRDTIVFVNAWAIHRDPNLWEDVTSFKPERHETANE 425
           L P APML+PH ASEDC ++GYDVPR TI+ VNAWAIHRDP LWE+   FKPER E   E
Sbjct: 371 LYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEKKGE 430

Query: 426 SFDSYKLLPFGLGRRACPGVGMAQRVVGLTLASLIQCFDWERMSSSLVDMTEGQ-GITMP 485
                KL+PFG+GRR+CPG G+AQR+V L L SL+QCF+WER+    +DM E + G TM 
Sbjct: 431 ---DKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMR 490

Query: 486 KAQPLVAKCKPRPIMRALV 497
           KA  L A CK RPI+  ++
Sbjct: 491 KATSLQAMCKARPIVHKVL 504

The following BLAST results are available for this feature:

BLAST of Spg031173 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901746.1	7.1e-254	85.80	cytochrome P450 81Q32-like [Benincasa hispida]	[more]
XP_022985606.1	1.7e-247	84.29	cytochrome P450 81D1-like [Cucurbita maxima]	[more]
XP_023512110.1	1.9e-246	84.10	cytochrome P450 81D1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022943756.1	3.2e-246	84.06	cytochrome P450 81D1-like [Cucurbita moschata]	[more]
XP_011650030.1	1.1e-241	83.27	cytochrome P450 81Q32 [Cucumis sativus] >KGN63296.1 hypothetical protein Csa_022...	[more]

BLAST of Spg031173 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	4.1e-164	57.83	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
P93147	1.9e-148	51.72	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q6WNQ8	9.3e-148	54.40	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
Q6WNQ9	1.3e-146	53.01	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
Q9FG65	8.7e-146	54.31	Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1	[more]

BLAST of Spg031173 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J8Q1	8.2e-248	84.29	cytochrome P450 81D1-like OS=Cucurbita maxima OX=3661 GN=LOC111483619 PE=3 SV=1	[more]
A0A6J1FSK8	1.5e-246	84.06	cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448412 PE=3 SV=...	[more]
A0A0A0LTD0	5.1e-242	83.27	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425740 PE=3 SV=1	[more]
A0A6J1D5S6	1.1e-212	71.83	isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE...	[more]
A0A6J1D5S1	8.5e-205	70.86	cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV...	[more]

BLAST of Spg031173 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37370.1	4.7e-147	52.30	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT5G36220.1	6.2e-147	54.31	cytochrome p450 81d1	[more]
AT4G37330.1	8.9e-146	52.00	cytochrome P450, family 81, subfamily D, polypeptide 4	[more]
AT4G37320.1	7.5e-145	50.41	cytochrome P450, family 81, subfamily D, polypeptide 5	[more]
AT3G28740.1	4.6e-142	51.10	Cytochrome P450 superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 435..458 score: 33.88 coord: 177..195 score: 26.73 coord: 287..304 score: 27.96 coord: 391..415 score: 31.01 coord: 425..435 score: 56.69 coord: 350..368 score: 35.17 coord: 58..77 score: 39.38 coord: 307..333 score: 34.63 coord: 82..103 score: 22.41
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 351..362 score: 39.56 coord: 426..435 score: 60.23 coord: 298..315 score: 41.57
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 30..479 e-value: 1.2E-89 score: 301.2
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 23..493 e-value: 1.7E-117 score: 394.9
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 30..489
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 6..461 e-value: 5.4E-18 score: 63.0
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 6..494
None	No IPR available	PANTHER	PTHR24298:SF836	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATED	coord: 6..494
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 428..437

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg031173.1	Spg031173.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen