Homology
BLAST of Spg031144 vs. NCBI nr
Match:
XP_022947035.1 (cytochrome P450 81F3 [Cucurbita moschata])
HSP 1 Score: 775.8 bits (2002), Expect = 2.3e-220
Identity = 383/502 (76.29%), Postives = 435/502 (86.65%), Query Frame = 0
Query: 1 MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
MDILLLSI + L SLL AFNFL PHRLNLPPTP FSLPLIGH HLLKHPLHR+LH LSQ
Sbjct: 1 MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQ 60
Query: 61 KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
K+GH+ SLRFGSRLVV++SSPSAV EC +KNDI+LANRPLL+TGK+LAYNHTT+A SPYG
Sbjct: 61 KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYG 120
Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMF 180
+HWRNLRRI SLEIFST+RLN+F+GIRE+EVKRLL KLC D EEFR VELEPML DL F
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSEEFREVELEPMLSDLTF 180
Query: 181 NIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMN 240
N++M M+ G +F D+G S+ F++LVTQIMAH G++NPG+FIP+WNW+DPTGF+KRIMN
Sbjct: 181 NVVMGMVGGRRF--YDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMN 240
Query: 241 VGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDT 300
+ +R D LQ LIDE R+ KD GNTMI LLCLQK EPEYYSD IIKGLIQVI LAGIDT
Sbjct: 241 LARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDT 300
Query: 301 TAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPA 360
+AVTLEWALS LLNNP VLKKA+AEID LIG+ERLVNE+D+P+L YL+GIISETLRLNPA
Sbjct: 301 SAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRLNPA 360
Query: 361 APLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGD 420
APLL+PHCAF+D KIEGYDVPRDTI+LIN WAIH +PNLWED T F+PERH +++ D
Sbjct: 361 APLLVPHCAFQDCKIEGYDVPRDTIVLINTWAIHRDPNLWEDPTAFKPERHGDTSG--AD 420
Query: 421 SCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQP 480
S KL+PFGLGRRACPGVG+AQRVVGL LASLIQ FEWER S SLVDM EG+GLTMPKAQP
Sbjct: 421 SYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQP 480
Query: 481 LVAKCKPRPIMKAILNAPTGNI 503
LVAKCKPRPIMKAI+N NI
Sbjct: 481 LVAKCKPRPIMKAIMNVEIDNI 498
BLAST of Spg031144 vs. NCBI nr
Match:
XP_023007415.1 (cytochrome P450 81F3-like [Cucurbita maxima])
HSP 1 Score: 775.8 bits (2002), Expect = 2.3e-220
Identity = 384/502 (76.49%), Postives = 436/502 (86.85%), Query Frame = 0
Query: 1 MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
MDILLLSI + L SLL AFNFL PHRLNLPPTP FSLPLIGH HLLKHPLHR+LH LSQ
Sbjct: 1 MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHNLSQ 60
Query: 61 KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
K+GH+ SLRFGSRLVV++SSPSAV EC +KNDI+LANRPLL+TGK+LAYNHTT+A SPYG
Sbjct: 61 KHGHVISLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120
Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMF 180
+HWRNLRRI SLEIFSTSRLNLFLGIR++EVKRLL KLC D EEFR VE+EPML DL F
Sbjct: 121 EHWRNLRRICSLEIFSTSRLNLFLGIRQNEVKRLLRKLCGDWLEEFREVEVEPMLSDLTF 180
Query: 181 NIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMN 240
N++M M+ G +F D+G S+ F++LVTQIMAH G++NPG+FIP+WNW+DPTGF+KRIMN
Sbjct: 181 NVVMGMVGGRRF--YDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMN 240
Query: 241 VGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDT 300
+ +R D LQ LIDE R+ KD GN+MI LLCLQK EPEYYSD IIKGLIQVI LAGIDT
Sbjct: 241 LARRGDELLQELIDEARDGKDGGNSMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDT 300
Query: 301 TAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPA 360
+AVTLEWALS LLNNP VLKKA+AEID LIG+ERLVNE+D+P+L YL+GIISETLRLNPA
Sbjct: 301 SAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLRYLQGIISETLRLNPA 360
Query: 361 APLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGD 420
APLL+PHCAF+D KIEGYDVPRDTI+LINAWAIH +P+LWED T F+PERH +S+ D
Sbjct: 361 APLLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPDLWEDPTAFKPERHGDSSG--AD 420
Query: 421 SCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQP 480
S KL+PFGLGRRACPGVG+AQRVVGL LASLIQ FEWER S SLVDM EG+GLTMPKAQP
Sbjct: 421 SYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQP 480
Query: 481 LVAKCKPRPIMKAILNAPTGNI 503
LVAKCKPRPIMKAI+N NI
Sbjct: 481 LVAKCKPRPIMKAIMNVEIDNI 498
BLAST of Spg031144 vs. NCBI nr
Match:
XP_023532689.1 (cytochrome P450 81F3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532690.1 cytochrome P450 81F3-like isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 773.1 bits (1995), Expect = 1.5e-219
Identity = 383/502 (76.29%), Postives = 435/502 (86.65%), Query Frame = 0
Query: 1 MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
MDILLLSI + L SLL AFNFL PHRLNLPPTP FSLPLIGH HLLKHPLHR+LH LSQ
Sbjct: 1 MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHTLSQ 60
Query: 61 KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
K+GH+ SLRFGSRLVV++SSPSAV EC +KNDI+LANRPLL+TGK+LAYNHTT+A SPYG
Sbjct: 61 KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120
Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMF 180
+HWRNLRRI SLEIFST+RLN+F+GIRE+EVKRLL KLC EE R VELEPML DL F
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGGWLEEGREVELEPMLSDLTF 180
Query: 181 NIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMN 240
N++M M+ G +F D+G S+ F++LVTQIMAH G++NPG+FIP+WNW+DPTGF+KRIMN
Sbjct: 181 NVVMGMVGGRRF--YDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMN 240
Query: 241 VGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDT 300
+ +R D LQ LIDE R+ KD GNTMI LLCLQK EPEYYSD IIKGLIQVI LAGIDT
Sbjct: 241 LARRGDEVLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDT 300
Query: 301 TAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPA 360
+AVTLEWALSQLLNNP VLKKA+AEID LIG+ERLVNE+D+P+L YL+GIISETLRLNPA
Sbjct: 301 SAVTLEWALSQLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRLNPA 360
Query: 361 APLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGD 420
APLL+PHCAF+D KIEGYDVPRDTI+LINAWAIH +PNLWED T F+PERH +++ D
Sbjct: 361 APLLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPNLWEDPTAFKPERHGDTSG--AD 420
Query: 421 SCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQP 480
S KL+PFGLGRRACPGVG+AQRVVGL LASLIQ FEWER S SLVDM EG+GLTMPKAQP
Sbjct: 421 SYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQP 480
Query: 481 LVAKCKPRPIMKAILNAPTGNI 503
LVAKCKPRPIMKAI+N NI
Sbjct: 481 LVAKCKPRPIMKAIMNVEIDNI 498
BLAST of Spg031144 vs. NCBI nr
Match:
XP_022149455.1 (cytochrome P450 81E8-like [Momordica charantia])
HSP 1 Score: 766.9 bits (1979), Expect = 1.1e-217
Identity = 378/496 (76.21%), Postives = 429/496 (86.49%), Query Frame = 0
Query: 1 MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
MDIL LSIS+ LFSLLLAF+FL R R NLPP+P FSLP++GHLHL+KHP+H+T H+LS
Sbjct: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
Query: 61 KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
KYGH+FSLRFGSR+VV+VSSPSAV EC +KNDIVLANRPLL +GK LAYNHTTMA +PYG
Sbjct: 61 KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120
Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMF 180
+HWRNLRRI +LEIFST+RLN+FLGIRE EV+ L+ KLC +S+EEF VELEPMLFDL F
Sbjct: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180
Query: 181 NIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMN 240
NI+MRM+ G K+ E S+ F+E+VTQ++AH G NPG+FIPIWNWIDPTGFEKRI+
Sbjct: 181 NIVMRMVAGKKYYEEQ--QSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIK 240
Query: 241 VGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDT 300
+GQR+D LQ LIDEIRN++ NTMID LLCLQK EPEYYSDQIIKGLIQVILLAGIDT
Sbjct: 241 LGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDT 300
Query: 301 TAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPA 360
AVTLEWALS+LLNNP+VL+KAKAEIDC +GQERLVNEAD+ SL YL+GII ETLRLNPA
Sbjct: 301 AAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPA 360
Query: 361 APLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGD 420
APLL+PHCA D KI GYDVPRDTIILIN WAIH +PNLWEDATNF+PERH N+ + D
Sbjct: 361 APLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGV--D 420
Query: 421 SCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQP 480
S KL+PFG GRR+CPGVG+AQRVVGLTLASLIQ FEWER S SLVDM EGKGLTMPKAQ
Sbjct: 421 SYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQH 480
Query: 481 LVAKCKPRPIMKAILN 497
LVAKCKPRPIMK ILN
Sbjct: 481 LVAKCKPRPIMKLILN 492
BLAST of Spg031144 vs. NCBI nr
Match:
XP_023512186.1 (isoflavone 2'-hydroxylase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512187.1 isoflavone 2'-hydroxylase-like isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 757.3 bits (1954), Expect = 8.4e-215
Identity = 371/495 (74.95%), Postives = 428/495 (86.46%), Query Frame = 0
Query: 1 MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
MDILLLSIS+F+F LL AFNF+L+PHRLNLPP+P F LP+IGHLHLL HP+HRTL LSQ
Sbjct: 1 MDILLLSISLFIFPLLFAFNFVLKPHRLNLPPSPLFCLPIIGHLHLLNHPIHRTLTNLSQ 60
Query: 61 KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
KYGH+FSLRFGSRLVV++SSPSAV EC +KNDIVLANRPLLN+ K++ YNHT M+ SPYG
Sbjct: 61 KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPLLNSAKYIDYNHTVMSVSPYG 120
Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEE-FRLVELEPMLFDLM 180
+HWRNLRRI+SLEIFST+RLNLFLGIRE E+K LLCKLC DS E+ FR+VELEPML DLM
Sbjct: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
Query: 181 FNIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIM 240
FNI+ RM+ G +F D F+E+VTQIMAH+G NPG+FIP+W +IDP+GFEKR+
Sbjct: 181 FNIVTRMVAGKRFYERHD----KFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVK 240
Query: 241 NVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGID 300
+G+ D L+ L+DEIR+K D GNTMI RLL LQ TEPEYYSDQIIKGLIQ ILLAGID
Sbjct: 241 KLGKTCDEILRELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGID 300
Query: 301 TTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNP 360
T AVTLEWALSQLLN+P+VL+KA+AEID IG ERLVNE D+P+L+Y++GIISET+RLNP
Sbjct: 301 TLAVTLEWALSQLLNHPEVLEKARAEIDNSIGYERLVNEVDLPNLSYVQGIISETMRLNP 360
Query: 361 AAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQG 420
AAPLL+PHCAFE+ KIEGYDVPRDTIILINAWA+H +PNLWEDAT+F+PERHAN
Sbjct: 361 AAPLLVPHCAFEECKIEGYDVPRDTIILINAWAMHRDPNLWEDATSFKPERHANGMKNGA 420
Query: 421 DSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQ 480
DS KLIPFGLGRRACPGVG+AQRV GLTLASLIQ FEWER + SLVDM EGKG+TMPKAQ
Sbjct: 421 DSYKLIPFGLGRRACPGVGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQ 480
Query: 481 PLVAKCKPRPIMKAI 495
PLVAKCKPRPIMK +
Sbjct: 481 PLVAKCKPRPIMKLV 491
BLAST of Spg031144 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 546.2 bits (1406), Expect = 3.9e-154
Identity = 267/496 (53.83%), Postives = 361/496 (72.78%), Query Frame = 0
Query: 3 ILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQKY 62
++LLSIS+ LF + R NLPP+P +LP+IGHLHL+ LHR+L+ LS+KY
Sbjct: 13 VVLLSISLKLFP--------VSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKY 72
Query: 63 GHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYGDH 122
G +FSL+ G+RLV++VSSP+A EC +KNDIV ANRPL GK++ YN+TTM SPYG+H
Sbjct: 73 GSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEH 132
Query: 123 WRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMFNI 182
WRNLRR+A++EIFS LN FL IRE EVK+LL L S ++F VE++ L +L FN+
Sbjct: 133 WRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNV 192
Query: 183 IMRMMVGNKFNSEDDGNSEG--FKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMN 242
MRM+ G ++ +D + E F+ L+ ++ HAG +NPG+F+P WID +EK++
Sbjct: 193 TMRMVAGKRYFGQDVDSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVSK 252
Query: 243 VGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDT 302
+ Q DA LQ LI E R K+ TMID LL LQ+++PEYY+DQIIKG+I V+LLAG DT
Sbjct: 253 ISQEMDAFLQRLIHESRINKN-NVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTDT 312
Query: 303 TAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPA 362
+AVT+EWA+S LLN+P+ L+KA+ EI+ +G RL+ E D+P L YL IISET RL PA
Sbjct: 313 SAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCPA 372
Query: 363 APLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGD 422
AP+L+PH + +D K++GYDVP+ TI+L+NAWAIH +P W++ T F+PERH ++ +
Sbjct: 373 APMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERH---GGVELE 432
Query: 423 SCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQP 482
KL+PFG+GRR+CPG G+AQRVVGLTL +LIQCFEW+R + +DM EG GLTMPKAQP
Sbjct: 433 PSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQP 492
Query: 483 LVAKCKPRPIMKAILN 497
L A CKPR I+ +++
Sbjct: 493 LEALCKPRNILHKVVS 496
BLAST of Spg031144 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 512.3 bits (1318), Expect = 6.2e-144
Identity = 258/501 (51.50%), Postives = 354/501 (70.66%), Query Frame = 0
Query: 1 MDIL-LLSISVFLFSLLLAFNFLLRPHRL-NLPPTPPFSLPLIGHLHLLKHPLHRTLHKL 60
M+IL LLS SVF +L FN ++R + NLPP PP SLP+IG+LH LK PLHRT L
Sbjct: 1 MEILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPP-SLPIIGNLHHLKRPLHRTFKGL 60
Query: 61 SQKYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASP 120
S+KYGH+FSL FGSRLVV+VSS S +C +KND+VLANRP +GK++ YN+TT+ ++
Sbjct: 61 SEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTS 120
Query: 121 YGDHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDL 180
YG+HWRNLRRI +L++ S R+N F GIR E +RL+ +L DS F +EL L+D+
Sbjct: 121 YGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDM 180
Query: 181 MFNIIMRMMVGNKFNSEDDGNSE-----GFKELVTQIMAHAGTANPGNFIPIWNWIDPTG 240
FN IMRM+ G ++ ED S+ F+++V++++ +G N +F+P+ ++D
Sbjct: 181 TFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFEN 240
Query: 241 FEKRIMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVI 300
EKR+ ++ + DA L+GLI+E R KK+ NTMID LL LQ ++PEYY+DQIIKGL +
Sbjct: 241 LEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAM 300
Query: 301 LLAGIDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISE 360
LLAG D++AVTLEW++S LLN+P+VLKK K E+D +GQ+RLV+E+D+P L YL+ +I+E
Sbjct: 301 LLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINE 360
Query: 361 TLRLNPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHAN 420
TLRL APLLLPH ++ I GY VP+DTI+LINAWAIH +P LW +AT F+PER
Sbjct: 361 TLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEK 420
Query: 421 SNAIQGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGL 480
+G+ KLI FG+GRRACPG G+A R + +TLA LIQCF+W+ +G +D+ E G
Sbjct: 421 ----KGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGF 480
Query: 481 TMPKAQPLVAKCKPRPIMKAI 495
T+ K PL A CK RP++ +
Sbjct: 481 TLTKLVPLKAMCKSRPVINKV 496
BLAST of Spg031144 vs. ExPASy Swiss-Prot
Match:
Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)
HSP 1 Score: 506.1 bits (1302), Expect = 4.4e-142
Identity = 262/503 (52.09%), Postives = 350/503 (69.58%), Query Frame = 0
Query: 3 ILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQKY 62
++L SI +F L+++F F L+P + NLPP+PP LP+IGHL LLK P+HRTL S+
Sbjct: 8 VVLYSIFSLIF-LIISFKF-LKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETL 67
Query: 63 GH-----LFSLRFGSRLVVIVSSPS-AVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAA 122
H + SLR GSRLV +VSS A EC KND+VLANRP + GKH+ YN+T M A
Sbjct: 68 DHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIA 127
Query: 123 SPYGDHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLF 182
+PYGDHWRNLRR+ ++EIFST RLN FL +R EV+RL+ +L + + +VEL+PML
Sbjct: 128 APYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLM 187
Query: 183 DLMFNIIMRMMVGNKFNSE---DDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTG 242
DL FN IMRMM G ++ E D+ ++ ++LV + A+ + N +++PI +
Sbjct: 188 DLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SS 247
Query: 243 FEKRIMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVI 302
+E R+ +G+ D LQGLID+ R +++ G TMID LL LQK++ EYY+DQIIKG+I ++
Sbjct: 248 YENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIM 307
Query: 303 LLAGIDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISE 362
++AG +T+AVTLEWALS LLN+P V+ KA+ EID +G +RL+ EAD+ L YL+ I+ E
Sbjct: 308 VIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLE 367
Query: 363 TLRLNPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHAN 422
TLRL+PA PLL+PH A ED KI YD+PR T +L+NAWAIH +PN W+D +F+PER
Sbjct: 368 TLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK 427
Query: 423 SNAIQGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGL 482
Q KL+ FGLGRRACPG G+AQR+VGL L SLIQCFEWER VDM+EG G
Sbjct: 428 EEEAQ----KLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGN 487
Query: 483 TMPKAQPLVAKCKPRPIMKAILN 497
T+PKA PL A CK RP + I++
Sbjct: 488 TVPKAIPLKAICKARPFLHKIIS 502
BLAST of Spg031144 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 500.7 bits (1288), Expect = 1.9e-140
Identity = 260/499 (52.10%), Postives = 344/499 (68.94%), Query Frame = 0
Query: 5 LLSISVFLFSLLLAFNFLLR--PHRL-NLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQK 64
L S+ S ++ LL+ RL NLPP PP ++P+IG+LH LKHPLHRT LSQ
Sbjct: 2 LFYYSLLSLSFIITIKILLKITSRRLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQT 61
Query: 65 YGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYGD 124
YG +FSL FGSRLVV+VSSPS HEC +KNDI+LANRP TGK++ YN+TT+ ++ YGD
Sbjct: 62 YGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGD 121
Query: 125 HWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEE---FRLVELEPMLFDL 184
HWRNLRRI ++++ S +RLN FLG+R E RL+ KL D E F VEL P L ++
Sbjct: 122 HWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEM 181
Query: 185 MFNIIMRMMVGNKFNSEDDGNSE-----GFKELVTQIMAHAGTANPGNFIPIWNWIDPTG 244
FN +MRM+ G ++ +D S+ F+E+++++M+ G N G+F+P+ +D
Sbjct: 182 TFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDN 241
Query: 245 FEKRIMNVGQRADAQLQGLIDEIR--NKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQ 304
EKR + +R++A L+GLI+E R N +G TMID LL L +++PEYYSD +IKGLIQ
Sbjct: 242 LEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQ 301
Query: 305 VILLAGIDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGII 364
+LLAG DT+AVT+EW +S+LLN+P+VLKKAK E+D IG+ +LV+E D+ L YL+ II
Sbjct: 302 GMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNII 361
Query: 365 SETLRLNPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERH 424
SETLRL+P APLLLPH + ED I ++VP+DTIIL N W IH +P W DA +F+PER
Sbjct: 362 SETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERF 421
Query: 425 ANSNAIQGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGK 484
+ K++ FGLGRRACPG+ +AQR VG T+ LIQCFEWER S +DM EGK
Sbjct: 422 EKEEEVN----KVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGK 481
Query: 485 GLTMPKAQPLVAKCKPRPI 491
G+TMP PL A CK PI
Sbjct: 482 GITMPMKIPLRAMCKALPI 495
BLAST of Spg031144 vs. ExPASy Swiss-Prot
Match:
Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)
HSP 1 Score: 500.0 bits (1286), Expect = 3.2e-140
Identity = 258/505 (51.09%), Postives = 346/505 (68.51%), Query Frame = 0
Query: 1 MDILLLSISVFLFSLLLAFNFLL--RPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKL 60
M+ + L +S+F F + L+ L RP +LNLPP+P P+IGHLHLLK PLHR L
Sbjct: 8 METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67
Query: 61 SQKYGH--LFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAA 120
S+ + +FSL GSRLV +VSS + EC +KND+VLANRP GKH+ YN TTM
Sbjct: 68 SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127
Query: 121 SPYGDHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLF 180
+ YGD WRNLRRI ++EIFS+ RLN F+ IR+ E++RL+ L +S F VE++P+
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFM 187
Query: 181 DLMFNIIMRMMVGNKF---NSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTG 240
L N I+RM+ G +F +E+D ++ ++L+ +++ G N ++ PI ++ T
Sbjct: 188 CLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TN 247
Query: 241 FEKRIMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVI 300
+EK + + R D LQ L++E R +K +GNTMID LL LQ+T+P+YY+D IIKG+I V+
Sbjct: 248 YEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVM 307
Query: 301 LLAGIDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISE 360
+LAG DT+A TLEWA+S LLN+P+VL+KAK EID IG +RLV E DI L YL+ I+SE
Sbjct: 308 ILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSE 367
Query: 361 TLRLNPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHAN 420
TLRL P AP+LLPH A ED ++GYDVPR TIIL+NAWAIH +P LWE+ F+PER
Sbjct: 368 TLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEK 427
Query: 421 SNAIQGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREG-KG 480
+G+ KL+PFG+GRR+CPG G+AQR+V L L SL+QCFEWER +DMRE KG
Sbjct: 428 ----KGEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKG 487
Query: 481 LTMPKAQPLVAKCKPRPIMKAILNA 498
TM KA L A CK RPI+ +L+A
Sbjct: 488 TTMRKATSLQAMCKARPIVHKVLDA 506
BLAST of Spg031144 vs. ExPASy TrEMBL
Match:
A0A6J1L2W8 (cytochrome P450 81F3-like OS=Cucurbita maxima OX=3661 GN=LOC111499919 PE=3 SV=1)
HSP 1 Score: 775.8 bits (2002), Expect = 1.1e-220
Identity = 384/502 (76.49%), Postives = 436/502 (86.85%), Query Frame = 0
Query: 1 MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
MDILLLSI + L SLL AFNFL PHRLNLPPTP FSLPLIGH HLLKHPLHR+LH LSQ
Sbjct: 1 MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHNLSQ 60
Query: 61 KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
K+GH+ SLRFGSRLVV++SSPSAV EC +KNDI+LANRPLL+TGK+LAYNHTT+A SPYG
Sbjct: 61 KHGHVISLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120
Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMF 180
+HWRNLRRI SLEIFSTSRLNLFLGIR++EVKRLL KLC D EEFR VE+EPML DL F
Sbjct: 121 EHWRNLRRICSLEIFSTSRLNLFLGIRQNEVKRLLRKLCGDWLEEFREVEVEPMLSDLTF 180
Query: 181 NIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMN 240
N++M M+ G +F D+G S+ F++LVTQIMAH G++NPG+FIP+WNW+DPTGF+KRIMN
Sbjct: 181 NVVMGMVGGRRF--YDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMN 240
Query: 241 VGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDT 300
+ +R D LQ LIDE R+ KD GN+MI LLCLQK EPEYYSD IIKGLIQVI LAGIDT
Sbjct: 241 LARRGDELLQELIDEARDGKDGGNSMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDT 300
Query: 301 TAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPA 360
+AVTLEWALS LLNNP VLKKA+AEID LIG+ERLVNE+D+P+L YL+GIISETLRLNPA
Sbjct: 301 SAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLRYLQGIISETLRLNPA 360
Query: 361 APLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGD 420
APLL+PHCAF+D KIEGYDVPRDTI+LINAWAIH +P+LWED T F+PERH +S+ D
Sbjct: 361 APLLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPDLWEDPTAFKPERHGDSSG--AD 420
Query: 421 SCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQP 480
S KL+PFGLGRRACPGVG+AQRVVGL LASLIQ FEWER S SLVDM EG+GLTMPKAQP
Sbjct: 421 SYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQP 480
Query: 481 LVAKCKPRPIMKAILNAPTGNI 503
LVAKCKPRPIMKAI+N NI
Sbjct: 481 LVAKCKPRPIMKAIMNVEIDNI 498
BLAST of Spg031144 vs. ExPASy TrEMBL
Match:
A0A6J1G5P7 (cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1)
HSP 1 Score: 775.8 bits (2002), Expect = 1.1e-220
Identity = 383/502 (76.29%), Postives = 435/502 (86.65%), Query Frame = 0
Query: 1 MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
MDILLLSI + L SLL AFNFL PHRLNLPPTP FSLPLIGH HLLKHPLHR+LH LSQ
Sbjct: 1 MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQ 60
Query: 61 KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
K+GH+ SLRFGSRLVV++SSPSAV EC +KNDI+LANRPLL+TGK+LAYNHTT+A SPYG
Sbjct: 61 KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYG 120
Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMF 180
+HWRNLRRI SLEIFST+RLN+F+GIRE+EVKRLL KLC D EEFR VELEPML DL F
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSEEFREVELEPMLSDLTF 180
Query: 181 NIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMN 240
N++M M+ G +F D+G S+ F++LVTQIMAH G++NPG+FIP+WNW+DPTGF+KRIMN
Sbjct: 181 NVVMGMVGGRRF--YDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMN 240
Query: 241 VGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDT 300
+ +R D LQ LIDE R+ KD GNTMI LLCLQK EPEYYSD IIKGLIQVI LAGIDT
Sbjct: 241 LARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDT 300
Query: 301 TAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPA 360
+AVTLEWALS LLNNP VLKKA+AEID LIG+ERLVNE+D+P+L YL+GIISETLRLNPA
Sbjct: 301 SAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRLNPA 360
Query: 361 APLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGD 420
APLL+PHCAF+D KIEGYDVPRDTI+LIN WAIH +PNLWED T F+PERH +++ D
Sbjct: 361 APLLVPHCAFQDCKIEGYDVPRDTIVLINTWAIHRDPNLWEDPTAFKPERHGDTSG--AD 420
Query: 421 SCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQP 480
S KL+PFGLGRRACPGVG+AQRVVGL LASLIQ FEWER S SLVDM EG+GLTMPKAQP
Sbjct: 421 SYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQP 480
Query: 481 LVAKCKPRPIMKAILNAPTGNI 503
LVAKCKPRPIMKAI+N NI
Sbjct: 481 LVAKCKPRPIMKAIMNVEIDNI 498
BLAST of Spg031144 vs. ExPASy TrEMBL
Match:
A0A6J1D5S1 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV=1)
HSP 1 Score: 766.9 bits (1979), Expect = 5.2e-218
Identity = 378/496 (76.21%), Postives = 429/496 (86.49%), Query Frame = 0
Query: 1 MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
MDIL LSIS+ LFSLLLAF+FL R R NLPP+P FSLP++GHLHL+KHP+H+T H+LS
Sbjct: 1 MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60
Query: 61 KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
KYGH+FSLRFGSR+VV+VSSPSAV EC +KNDIVLANRPLL +GK LAYNHTTMA +PYG
Sbjct: 61 KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120
Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMF 180
+HWRNLRRI +LEIFST+RLN+FLGIRE EV+ L+ KLC +S+EEF VELEPMLFDL F
Sbjct: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180
Query: 181 NIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMN 240
NI+MRM+ G K+ E S+ F+E+VTQ++AH G NPG+FIPIWNWIDPTGFEKRI+
Sbjct: 181 NIVMRMVAGKKYYEEQ--QSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIK 240
Query: 241 VGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDT 300
+GQR+D LQ LIDEIRN++ NTMID LLCLQK EPEYYSDQIIKGLIQVILLAGIDT
Sbjct: 241 LGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDT 300
Query: 301 TAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPA 360
AVTLEWALS+LLNNP+VL+KAKAEIDC +GQERLVNEAD+ SL YL+GII ETLRLNPA
Sbjct: 301 AAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPA 360
Query: 361 APLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGD 420
APLL+PHCA D KI GYDVPRDTIILIN WAIH +PNLWEDATNF+PERH N+ + D
Sbjct: 361 APLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGV--D 420
Query: 421 SCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQP 480
S KL+PFG GRR+CPGVG+AQRVVGLTLASLIQ FEWER S SLVDM EGKGLTMPKAQ
Sbjct: 421 SYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQH 480
Query: 481 LVAKCKPRPIMKAILN 497
LVAKCKPRPIMK ILN
Sbjct: 481 LVAKCKPRPIMKLILN 492
BLAST of Spg031144 vs. ExPASy TrEMBL
Match:
A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)
HSP 1 Score: 755.4 bits (1949), Expect = 1.6e-214
Identity = 369/498 (74.10%), Postives = 435/498 (87.35%), Query Frame = 0
Query: 1 MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
MDILLLSIS+ LFSL + F+FLLRP+R NLPP+P F LPLIGHLHL+KHP+HRTLHKLS
Sbjct: 81 MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140
Query: 61 KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
YGH+FSLR+GSRLVV+VSSPSAV EC +KNDIVLANRPLL +GK LAYNHTT+A SPYG
Sbjct: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200
Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMF 180
+HWRNLRRI++LEIFSTSRLNLFLGIRE EVK LL KL DS +EFR VEL+ ML D F
Sbjct: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260
Query: 181 NIIMRMMVGNKFNSE---DDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKR 240
NI++RM+ G K+ E D+G S+ F+E+V +I+A+ GTAN G+F+P+WNWIDPTG+EKR
Sbjct: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320
Query: 241 IMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAG 300
IM +G+R+D LQ L+DEIRN+KD GNTMID LL LQ T+PEY+SDQIIKGL Q++LLAG
Sbjct: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380
Query: 301 IDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRL 360
IDT A+TLEWALSQLLNNP+VLKKA+AEID IG++RLV+E D+ SL+YL+G+I+ETLRL
Sbjct: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440
Query: 361 NPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAI 420
NPAAPLL+PHCA ED KIEGYD+PRDTIILINAWAIH + N+WEDAT+F+PERHAN+
Sbjct: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMG- 500
Query: 421 QGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPK 480
DS KL+PFGLGRRACPG+G+AQRVVGLTLASLIQCFEWER + SLVDM EG+GLTMPK
Sbjct: 501 GVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPK 560
Query: 481 AQPLVAKCKPRPIMKAIL 496
AQPLVAKCKPRPI+KAIL
Sbjct: 561 AQPLVAKCKPRPILKAIL 577
BLAST of Spg031144 vs. ExPASy TrEMBL
Match:
A0A6J1FXS5 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448183 PE=3 SV=1)
HSP 1 Score: 752.3 bits (1941), Expect = 1.3e-213
Identity = 371/495 (74.95%), Postives = 427/495 (86.26%), Query Frame = 0
Query: 1 MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
MDILLLSIS+FLF+LL AFNF+L+PHRLNLPP P LPLIGHLHLL HP+HRTL LSQ
Sbjct: 1 MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60
Query: 61 KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
KYGH+FSLRFGSRLVV++SSPSAV EC +KNDI LANRPLLN+GK+L YNHT M+ SPYG
Sbjct: 61 KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 120
Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEE-FRLVELEPMLFDLM 180
+HWRNLRRI SLEIFST+RLNLFLG+R+ E+K LLCKLC DS E+ FR+VELEPML DLM
Sbjct: 121 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
Query: 181 FNIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIM 240
FNI+M+M+ G K +G+S FKE+VTQIMAH+G NP +FIP+W +IDPTGF KR
Sbjct: 181 FNIVMKMVAGKK---SFEGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGK 240
Query: 241 NVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGID 300
+ + +D LQ L+DEIR+K+D GNTMI RLL LQ TEPEYYSDQIIKGLIQ ILLAGID
Sbjct: 241 KLAKTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGID 300
Query: 301 TTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNP 360
T AVTLEWA+SQLLNNP+VL+KA+AEID IG ERLVNE+D+PSL+Y++GIISETLRL+P
Sbjct: 301 TIAVTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHP 360
Query: 361 AAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQG 420
AAPLLLPHCAFE+ KIEGYDVPRDTII INAWA+H +PNLWEDAT+F+PERHAN
Sbjct: 361 AAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGA 420
Query: 421 DSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQ 480
+S KLIPFGLGRRACPG+G+AQRV GLTLASLIQ FEWER + SLVDM EGKG+TMPKAQ
Sbjct: 421 ESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQ 480
Query: 481 PLVAKCKPRPIMKAI 495
PLVAKCKPRPIMK +
Sbjct: 481 PLVAKCKPRPIMKLV 492
BLAST of Spg031144 vs. TAIR 10
Match:
AT5G36220.1 (cytochrome p450 81d1 )
HSP 1 Score: 506.1 bits (1302), Expect = 3.2e-143
Identity = 262/503 (52.09%), Postives = 350/503 (69.58%), Query Frame = 0
Query: 3 ILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQKY 62
++L SI +F L+++F F L+P + NLPP+PP LP+IGHL LLK P+HRTL S+
Sbjct: 8 VVLYSIFSLIF-LIISFKF-LKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETL 67
Query: 63 GH-----LFSLRFGSRLVVIVSSPS-AVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAA 122
H + SLR GSRLV +VSS A EC KND+VLANRP + GKH+ YN+T M A
Sbjct: 68 DHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIA 127
Query: 123 SPYGDHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLF 182
+PYGDHWRNLRR+ ++EIFST RLN FL +R EV+RL+ +L + + +VEL+PML
Sbjct: 128 APYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLM 187
Query: 183 DLMFNIIMRMMVGNKFNSE---DDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTG 242
DL FN IMRMM G ++ E D+ ++ ++LV + A+ + N +++PI +
Sbjct: 188 DLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SS 247
Query: 243 FEKRIMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVI 302
+E R+ +G+ D LQGLID+ R +++ G TMID LL LQK++ EYY+DQIIKG+I ++
Sbjct: 248 YENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIM 307
Query: 303 LLAGIDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISE 362
++AG +T+AVTLEWALS LLN+P V+ KA+ EID +G +RL+ EAD+ L YL+ I+ E
Sbjct: 308 VIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLE 367
Query: 363 TLRLNPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHAN 422
TLRL+PA PLL+PH A ED KI YD+PR T +L+NAWAIH +PN W+D +F+PER
Sbjct: 368 TLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK 427
Query: 423 SNAIQGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGL 482
Q KL+ FGLGRRACPG G+AQR+VGL L SLIQCFEWER VDM+EG G
Sbjct: 428 EEEAQ----KLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGN 487
Query: 483 TMPKAQPLVAKCKPRPIMKAILN 497
T+PKA PL A CK RP + I++
Sbjct: 488 TVPKAIPLKAICKARPFLHKIIS 502
BLAST of Spg031144 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 503.1 bits (1294), Expect = 2.7e-142
Identity = 260/498 (52.21%), Postives = 353/498 (70.88%), Query Frame = 0
Query: 4 LLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQKYG 63
L+ SI + SL+ L R + NLPP+P +SLP+IGHL LLK P+HRT LSQ
Sbjct: 6 LIFSILFVVLSLIYLIGKLKR--KPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLN 65
Query: 64 H--LFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYGD 123
+ +FSLR G+RLV + SS S EC +KND+VLANRP KH+AY++TTM A+ YGD
Sbjct: 66 NAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGD 125
Query: 124 HWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMFN 183
HWRNLRRI S+EIFS RLN FL IR+ E++RL+ +L + +EF V+++ ML DL FN
Sbjct: 126 HWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFN 185
Query: 184 IIMRMMVGNKF---NSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRI 243
I+RM+ G ++ EDD ++ ++L+ ++A AG N +++P+ + + +E R+
Sbjct: 186 NILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRV 245
Query: 244 MNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGI 303
+ R D LQGL+DE R K++GNTMID LL LQ+++P+Y++D+IIKG + ++LAG
Sbjct: 246 KKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGT 305
Query: 304 DTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLN 363
DT+AVTLEWALS +LN+P VL KA+ EID IG +RL++E+DI +L YL+ I+SETLRL
Sbjct: 306 DTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLY 365
Query: 364 PAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQ 423
PAAP+LLPH A ED K+ GYD+PR TI+L N WAIH +P LW+D +F+PER +
Sbjct: 366 PAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEK----E 425
Query: 424 GDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKA 483
G++ KL+PFGLGRRACPG G+A R++ LTL SLIQC EWE+ G VDM EGKG+TMPKA
Sbjct: 426 GEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKI-GEEVDMSEGKGVTMPKA 485
Query: 484 QPLVAKCKPRPIMKAILN 497
+PL A C+ RP + I N
Sbjct: 486 KPLEAMCRARPSVVKIFN 494
BLAST of Spg031144 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 501.5 bits (1290), Expect = 7.8e-142
Identity = 255/498 (51.20%), Postives = 346/498 (69.48%), Query Frame = 0
Query: 1 MDILLLSISVFLFSLLLAFNFLLRPHR-LNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLS 60
MD+ + I FL LA L R R LNLPP+P SLP+IGHLHLLK PLHRT LS
Sbjct: 1 MDLNQILILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLS 60
Query: 61 QKYGH--LFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAAS 120
+ G+ +F LR G+RLV ++SS S EC +KND+VLANRP KHL YN T + ++
Sbjct: 61 KSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSA 120
Query: 121 PYGDHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFD 180
YGDHWRNLRRIA++EIFST RLN FL IR+ E++RL+ L DS F VE++ +L +
Sbjct: 121 SYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTN 180
Query: 181 LMFNIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKR 240
L N +RM+ G ++ ED+ +++ K LV++ + AG NP +++ I W+ + +EKR
Sbjct: 181 LASNTTIRMLAGKRYFGEDNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKR 240
Query: 241 IMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAG 300
I N+G R D LQ L+DE R +K++G TMID LL LQ +P+YY+D IIKG+I +++AG
Sbjct: 241 IKNLGNRFDTFLQKLVDEKRAEKEKGETMIDHLLALQDIQPDYYTDVIIKGIILTLIIAG 300
Query: 301 IDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRL 360
DT++VTLEWA+S LLN+P++LKKA+ EID +G +RLV+E+DI +L+YL+ I+ ETLR+
Sbjct: 301 TDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLRM 360
Query: 361 NPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAI 420
PA PLLLPH + ED K+ GYD+P T++L NAWA+H +P +WED F+PER
Sbjct: 361 YPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEK---- 420
Query: 421 QGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPK 480
+G++ KLI FG+GRRACPG G+A R++ L SL+QCFEWER VDM E KG T+PK
Sbjct: 421 EGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPK 480
Query: 481 AQPLVAKCKPRPIMKAIL 496
A PL A CK R I+ ++
Sbjct: 481 AIPLRAMCKARSIVDKLI 492
BLAST of Spg031144 vs. TAIR 10
Match:
AT3G28740.1 (Cytochrome P450 superfamily protein )
HSP 1 Score: 500.0 bits (1286), Expect = 2.3e-141
Identity = 258/505 (51.09%), Postives = 346/505 (68.51%), Query Frame = 0
Query: 1 MDILLLSISVFLFSLLLAFNFLL--RPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKL 60
M+ + L +S+F F + L+ L RP +LNLPP+P P+IGHLHLLK PLHR L
Sbjct: 8 METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67
Query: 61 SQKYGH--LFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAA 120
S+ + +FSL GSRLV +VSS + EC +KND+VLANRP GKH+ YN TTM
Sbjct: 68 SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127
Query: 121 SPYGDHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLF 180
+ YGD WRNLRRI ++EIFS+ RLN F+ IR+ E++RL+ L +S F VE++P+
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFM 187
Query: 181 DLMFNIIMRMMVGNKF---NSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTG 240
L N I+RM+ G +F +E+D ++ ++L+ +++ G N ++ PI ++ T
Sbjct: 188 CLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TN 247
Query: 241 FEKRIMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVI 300
+EK + + R D LQ L++E R +K +GNTMID LL LQ+T+P+YY+D IIKG+I V+
Sbjct: 248 YEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVM 307
Query: 301 LLAGIDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISE 360
+LAG DT+A TLEWA+S LLN+P+VL+KAK EID IG +RLV E DI L YL+ I+SE
Sbjct: 308 ILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSE 367
Query: 361 TLRLNPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHAN 420
TLRL P AP+LLPH A ED ++GYDVPR TIIL+NAWAIH +P LWE+ F+PER
Sbjct: 368 TLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEK 427
Query: 421 SNAIQGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREG-KG 480
+G+ KL+PFG+GRR+CPG G+AQR+V L L SL+QCFEWER +DMRE KG
Sbjct: 428 ----KGEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKG 487
Query: 481 LTMPKAQPLVAKCKPRPIMKAILNA 498
TM KA L A CK RPI+ +L+A
Sbjct: 488 TTMRKATSLQAMCKARPIVHKVLDA 506
BLAST of Spg031144 vs. TAIR 10
Match:
AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )
HSP 1 Score: 499.6 bits (1285), Expect = 3.0e-141
Identity = 253/498 (50.80%), Postives = 351/498 (70.48%), Query Frame = 0
Query: 1 MDILLLSISVFLFSLLLAFNFLLRP--HRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKL 60
MD+ + + FLF L ++ LL + NLPP+P + LP+IGHLHLLK P+HRT H +
Sbjct: 1 MDLTQILLLSFLF-LTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSI 60
Query: 61 SQKYGH--LFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAA 120
S+ G+ +F LR G+RLV ++SS S EC +KND+VLANRP + KH+ YN T M A
Sbjct: 61 SKSLGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIA 120
Query: 121 SPYGDHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLF 180
+ YGDHWRNLRRIA++EIFS+ R++ F IR+ E++RL+ L DS F VEL+ +L
Sbjct: 121 ASYGDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLT 180
Query: 181 DLMFNIIMRMMVGNKF---NSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTG 240
+L FN I+ M+ G ++ +ED+ ++ +EL+ +IMA AG+ N +++P NW+ T
Sbjct: 181 NLAFNNIIMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TN 240
Query: 241 FEKRIMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVI 300
FE + +G R D LQ L+DE R +K++G T+ID LL Q+TEPEYY+D IIKG+I +
Sbjct: 241 FENQTKILGNRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILAL 300
Query: 301 LLAGIDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISE 360
+LAG DT++VTLEWA+S LLN+P++L+KA+AEID IG +RLV E+DI +L+YL+ I+SE
Sbjct: 301 VLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSE 360
Query: 361 TLRLNPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHAN 420
TLRL PA PLLLPH + ++ K+ GYD+PR T++L N WA+H +P LWE+ F+PER
Sbjct: 361 TLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERFEK 420
Query: 421 SNAIQGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGL 480
+G++ KL+PFG+GRRACPG + +R+V L L LIQ FEWER LVDM EG+G+
Sbjct: 421 ----EGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGI 480
Query: 481 TMPKAQPLVAKCKPRPIM 492
TMPKA PL A CK R I+
Sbjct: 481 TMPKATPLRAMCKARAIV 491
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022947035.1 | 2.3e-220 | 76.29 | cytochrome P450 81F3 [Cucurbita moschata] | [more] |
XP_023007415.1 | 2.3e-220 | 76.49 | cytochrome P450 81F3-like [Cucurbita maxima] | [more] |
XP_023532689.1 | 1.5e-219 | 76.29 | cytochrome P450 81F3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532690.... | [more] |
XP_022149455.1 | 1.1e-217 | 76.21 | cytochrome P450 81E8-like [Momordica charantia] | [more] |
XP_023512186.1 | 8.4e-215 | 74.95 | isoflavone 2'-hydroxylase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_02351... | [more] |
Match Name | E-value | Identity | Description | |
W8JMU7 | 3.9e-154 | 53.83 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
P93147 | 6.2e-144 | 51.50 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
Q9FG65 | 4.4e-142 | 52.09 | Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1 | [more] |
Q6WNQ9 | 1.9e-140 | 52.10 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
Q9LHA1 | 3.2e-140 | 51.09 | Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1L2W8 | 1.1e-220 | 76.49 | cytochrome P450 81F3-like OS=Cucurbita maxima OX=3661 GN=LOC111499919 PE=3 SV=1 | [more] |
A0A6J1G5P7 | 1.1e-220 | 76.29 | cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1 | [more] |
A0A6J1D5S1 | 5.2e-218 | 76.21 | cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV... | [more] |
A0A6J1D5S6 | 1.6e-214 | 74.10 | isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE... | [more] |
A0A6J1FXS5 | 1.3e-213 | 74.95 | isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448183 PE=... | [more] |