Spg031144 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg031144
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptioncytochrome P450 81F3
Locationscaffold8: 30508446 .. 30510262 (+)
RNA-Seq ExpressionSpg031144
SyntenySpg031144
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGTGCATTACAATAAACCTTACCCATATCCCTATATATATACACACATGAAGATCGAAGTTCCTTCATGAAAAACACCAGAAAGGAGAAATCAAAACCTAGTAGAGGGCGACCACATGGATATTCTTTTGCTTTCCATTTCTGTCTTTCTATTTTCTCTCCTTCTTGCCTTCAACTTTCTGCTGCGACCTCATCGCTTGAACCTCCCACCAACCCCACCTTTTTCCCTTCCACTAATAGGCCATCTCCATCTCCTCAAACACCCTCTCCATCGAACACTTCACAAACTCTCCCAAAAATATGGCCATCTCTTCTCTCTTCGATTTGGTTCACGTCTTGTCGTCATCGTATCATCTCCTTCTGCCGTGCACGAATGCCTTTCAAAGAACGACATCGTTCTTGCAAATCGGCCATTGTTGAACACCGGAAAGCACTTGGCATACAACCACACCACCATGGCGGCCTCCCCTTATGGTGACCATTGGCGAAACCTCCGCCGCATTGCCTCCCTCGAGATCTTCTCAACAAGCCGCCTCAATTTGTTCTTGGGAATTCGAGAACATGAAGTCAAACGTTTACTGTGTAAATTATGTCATGATTCGTGGGAAGAGTTTAGGCTCGTGGAATTGGAGCCGATGTTGTTTGATCTTATGTTCAATATTATTATGAGAATGATGGTTGGAAATAAGTTTAATAGTGAGGACGACGGAAATTCTGAGGGATTCAAAGAGTTGGTGACACAAATTATGGCACATGCTGGAACAGCAAATCCAGGAAACTTCATACCCATATGGAATTGGATTGATCCAACTGGTTTTGAGAAACGGATAATGAATGTTGGACAAAGGGCAGATGCGCAACTTCAAGGATTGATTGATGAGATTCGAAATAAAAAGGATGAAGGGAACACTATGATTGACCGTTTACTTTGCTTGCAGAAAACTGAGCCTGAATATTACAGTGACCAAATTATCAAAGGACTCATTCAAGTATATTTCTCTCTCTATTTCAAACCCTTTTTTTATGCTTTCTTCTTCATTGTTTTCTCTCTCTCTCTATTGAAAGTATTTGGTTCTTGTAGGTTATATTATTGGCAGGGATTGACACGACAGCTGTGACTTTAGAATGGGCACTATCTCAATTGCTCAACAATCCGCAGGTGTTAAAGAAAGCAAAGGCTGAGATAGACTGTTTGATTGGACAAGAGCGTCTAGTTAATGAAGCTGATATTCCAAGTTTGAATTATCTTCGAGGAATAATCTCTGAGACATTGCGGCTAAACCCAGCAGCTCCTCTCCTTTTGCCACACTGTGCATTTGAAGATTCCAAAATAGAAGGATATGATGTTCCACGTGACACAATTATATTGATTAATGCCTGGGCTATACATACAAACCCAAATCTATGGGAAGATGCCACCAATTTTGAACCTGAAAGGCATGCAAATTCAAATGCAATTCAGGGTGACTCATGCAAACTAATACCATTTGGGTTGGGGAGGAGGGCCTGTCCTGGTGTGGGAGTGGCGCAACGAGTGGTTGGCTTAACTTTAGCATCATTGATACAATGCTTCGAATGGGAAAGAGCGAGTGGCTCATTAGTTGACATGAGAGAAGGTAAAGGGCTAACAATGCCCAAAGCTCAACCGTTGGTAGCTAAGTGTAAACCACGTCCAATCATGAAAGCCATTCTCAATGCACCAACTGGCAATATTTGAAAATGGACTACAAAAGCTAGCAAAATGTCATTTGAAATAATGGAAATTCTTACTTTTTCCTTAAAAAAATATATGTATTGCCATAGTTTATAATTGAATT

mRNA sequence

ATGGATATTCTTTTGCTTTCCATTTCTGTCTTTCTATTTTCTCTCCTTCTTGCCTTCAACTTTCTGCTGCGACCTCATCGCTTGAACCTCCCACCAACCCCACCTTTTTCCCTTCCACTAATAGGCCATCTCCATCTCCTCAAACACCCTCTCCATCGAACACTTCACAAACTCTCCCAAAAATATGGCCATCTCTTCTCTCTTCGATTTGGTTCACGTCTTGTCGTCATCGTATCATCTCCTTCTGCCGTGCACGAATGCCTTTCAAAGAACGACATCGTTCTTGCAAATCGGCCATTGTTGAACACCGGAAAGCACTTGGCATACAACCACACCACCATGGCGGCCTCCCCTTATGGTGACCATTGGCGAAACCTCCGCCGCATTGCCTCCCTCGAGATCTTCTCAACAAGCCGCCTCAATTTGTTCTTGGGAATTCGAGAACATGAAGTCAAACGTTTACTGTGTAAATTATGTCATGATTCGTGGGAAGAGTTTAGGCTCGTGGAATTGGAGCCGATGTTGTTTGATCTTATGTTCAATATTATTATGAGAATGATGGTTGGAAATAAGTTTAATAGTGAGGACGACGGAAATTCTGAGGGATTCAAAGAGTTGGTGACACAAATTATGGCACATGCTGGAACAGCAAATCCAGGAAACTTCATACCCATATGGAATTGGATTGATCCAACTGGTTTTGAGAAACGGATAATGAATGTTGGACAAAGGGCAGATGCGCAACTTCAAGGATTGATTGATGAGATTCGAAATAAAAAGGATGAAGGGAACACTATGATTGACCGTTTACTTTGCTTGCAGAAAACTGAGCCTGAATATTACAGTGACCAAATTATCAAAGGACTCATTCAAGTTATATTATTGGCAGGGATTGACACGACAGCTGTGACTTTAGAATGGGCACTATCTCAATTGCTCAACAATCCGCAGGTGTTAAAGAAAGCAAAGGCTGAGATAGACTGTTTGATTGGACAAGAGCGTCTAGTTAATGAAGCTGATATTCCAAGTTTGAATTATCTTCGAGGAATAATCTCTGAGACATTGCGGCTAAACCCAGCAGCTCCTCTCCTTTTGCCACACTGTGCATTTGAAGATTCCAAAATAGAAGGATATGATGTTCCACGTGACACAATTATATTGATTAATGCCTGGGCTATACATACAAACCCAAATCTATGGGAAGATGCCACCAATTTTGAACCTGAAAGGCATGCAAATTCAAATGCAATTCAGGGTGACTCATGCAAACTAATACCATTTGGGTTGGGGAGGAGGGCCTGTCCTGGTGTGGGAGTGGCGCAACGAGTGGTTGGCTTAACTTTAGCATCATTGATACAATGCTTCGAATGGGAAAGAGCGAGTGGCTCATTAGTTGACATGAGAGAAGGTAAAGGGCTAACAATGCCCAAAGCTCAACCGTTGGTAGCTAAGTGTAAACCACGTCCAATCATGAAAGCCATTCTCAATGCACCAACTGGCAATATTTGA

Coding sequence (CDS)

ATGGATATTCTTTTGCTTTCCATTTCTGTCTTTCTATTTTCTCTCCTTCTTGCCTTCAACTTTCTGCTGCGACCTCATCGCTTGAACCTCCCACCAACCCCACCTTTTTCCCTTCCACTAATAGGCCATCTCCATCTCCTCAAACACCCTCTCCATCGAACACTTCACAAACTCTCCCAAAAATATGGCCATCTCTTCTCTCTTCGATTTGGTTCACGTCTTGTCGTCATCGTATCATCTCCTTCTGCCGTGCACGAATGCCTTTCAAAGAACGACATCGTTCTTGCAAATCGGCCATTGTTGAACACCGGAAAGCACTTGGCATACAACCACACCACCATGGCGGCCTCCCCTTATGGTGACCATTGGCGAAACCTCCGCCGCATTGCCTCCCTCGAGATCTTCTCAACAAGCCGCCTCAATTTGTTCTTGGGAATTCGAGAACATGAAGTCAAACGTTTACTGTGTAAATTATGTCATGATTCGTGGGAAGAGTTTAGGCTCGTGGAATTGGAGCCGATGTTGTTTGATCTTATGTTCAATATTATTATGAGAATGATGGTTGGAAATAAGTTTAATAGTGAGGACGACGGAAATTCTGAGGGATTCAAAGAGTTGGTGACACAAATTATGGCACATGCTGGAACAGCAAATCCAGGAAACTTCATACCCATATGGAATTGGATTGATCCAACTGGTTTTGAGAAACGGATAATGAATGTTGGACAAAGGGCAGATGCGCAACTTCAAGGATTGATTGATGAGATTCGAAATAAAAAGGATGAAGGGAACACTATGATTGACCGTTTACTTTGCTTGCAGAAAACTGAGCCTGAATATTACAGTGACCAAATTATCAAAGGACTCATTCAAGTTATATTATTGGCAGGGATTGACACGACAGCTGTGACTTTAGAATGGGCACTATCTCAATTGCTCAACAATCCGCAGGTGTTAAAGAAAGCAAAGGCTGAGATAGACTGTTTGATTGGACAAGAGCGTCTAGTTAATGAAGCTGATATTCCAAGTTTGAATTATCTTCGAGGAATAATCTCTGAGACATTGCGGCTAAACCCAGCAGCTCCTCTCCTTTTGCCACACTGTGCATTTGAAGATTCCAAAATAGAAGGATATGATGTTCCACGTGACACAATTATATTGATTAATGCCTGGGCTATACATACAAACCCAAATCTATGGGAAGATGCCACCAATTTTGAACCTGAAAGGCATGCAAATTCAAATGCAATTCAGGGTGACTCATGCAAACTAATACCATTTGGGTTGGGGAGGAGGGCCTGTCCTGGTGTGGGAGTGGCGCAACGAGTGGTTGGCTTAACTTTAGCATCATTGATACAATGCTTCGAATGGGAAAGAGCGAGTGGCTCATTAGTTGACATGAGAGAAGGTAAAGGGCTAACAATGCCCAAAGCTCAACCGTTGGTAGCTAAGTGTAAACCACGTCCAATCATGAAAGCCATTCTCAATGCACCAACTGGCAATATTTGA

Protein sequence

MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQKYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYGDHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMFNIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQPLVAKCKPRPIMKAILNAPTGNI
Homology
BLAST of Spg031144 vs. NCBI nr
Match: XP_022947035.1 (cytochrome P450 81F3 [Cucurbita moschata])

HSP 1 Score: 775.8 bits (2002), Expect = 2.3e-220
Identity = 383/502 (76.29%), Postives = 435/502 (86.65%), Query Frame = 0

Query: 1   MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
           MDILLLSI + L SLL AFNFL  PHRLNLPPTP FSLPLIGH HLLKHPLHR+LH LSQ
Sbjct: 1   MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQ 60

Query: 61  KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
           K+GH+ SLRFGSRLVV++SSPSAV EC +KNDI+LANRPLL+TGK+LAYNHTT+A SPYG
Sbjct: 61  KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYG 120

Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMF 180
           +HWRNLRRI SLEIFST+RLN+F+GIRE+EVKRLL KLC D  EEFR VELEPML DL F
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSEEFREVELEPMLSDLTF 180

Query: 181 NIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMN 240
           N++M M+ G +F   D+G S+ F++LVTQIMAH G++NPG+FIP+WNW+DPTGF+KRIMN
Sbjct: 181 NVVMGMVGGRRF--YDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMN 240

Query: 241 VGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDT 300
           + +R D  LQ LIDE R+ KD GNTMI  LLCLQK EPEYYSD IIKGLIQVI LAGIDT
Sbjct: 241 LARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDT 300

Query: 301 TAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPA 360
           +AVTLEWALS LLNNP VLKKA+AEID LIG+ERLVNE+D+P+L YL+GIISETLRLNPA
Sbjct: 301 SAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRLNPA 360

Query: 361 APLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGD 420
           APLL+PHCAF+D KIEGYDVPRDTI+LIN WAIH +PNLWED T F+PERH +++    D
Sbjct: 361 APLLVPHCAFQDCKIEGYDVPRDTIVLINTWAIHRDPNLWEDPTAFKPERHGDTSG--AD 420

Query: 421 SCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQP 480
           S KL+PFGLGRRACPGVG+AQRVVGL LASLIQ FEWER S SLVDM EG+GLTMPKAQP
Sbjct: 421 SYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQP 480

Query: 481 LVAKCKPRPIMKAILNAPTGNI 503
           LVAKCKPRPIMKAI+N    NI
Sbjct: 481 LVAKCKPRPIMKAIMNVEIDNI 498

BLAST of Spg031144 vs. NCBI nr
Match: XP_023007415.1 (cytochrome P450 81F3-like [Cucurbita maxima])

HSP 1 Score: 775.8 bits (2002), Expect = 2.3e-220
Identity = 384/502 (76.49%), Postives = 436/502 (86.85%), Query Frame = 0

Query: 1   MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
           MDILLLSI + L SLL AFNFL  PHRLNLPPTP FSLPLIGH HLLKHPLHR+LH LSQ
Sbjct: 1   MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHNLSQ 60

Query: 61  KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
           K+GH+ SLRFGSRLVV++SSPSAV EC +KNDI+LANRPLL+TGK+LAYNHTT+A SPYG
Sbjct: 61  KHGHVISLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120

Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMF 180
           +HWRNLRRI SLEIFSTSRLNLFLGIR++EVKRLL KLC D  EEFR VE+EPML DL F
Sbjct: 121 EHWRNLRRICSLEIFSTSRLNLFLGIRQNEVKRLLRKLCGDWLEEFREVEVEPMLSDLTF 180

Query: 181 NIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMN 240
           N++M M+ G +F   D+G S+ F++LVTQIMAH G++NPG+FIP+WNW+DPTGF+KRIMN
Sbjct: 181 NVVMGMVGGRRF--YDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMN 240

Query: 241 VGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDT 300
           + +R D  LQ LIDE R+ KD GN+MI  LLCLQK EPEYYSD IIKGLIQVI LAGIDT
Sbjct: 241 LARRGDELLQELIDEARDGKDGGNSMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDT 300

Query: 301 TAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPA 360
           +AVTLEWALS LLNNP VLKKA+AEID LIG+ERLVNE+D+P+L YL+GIISETLRLNPA
Sbjct: 301 SAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLRYLQGIISETLRLNPA 360

Query: 361 APLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGD 420
           APLL+PHCAF+D KIEGYDVPRDTI+LINAWAIH +P+LWED T F+PERH +S+    D
Sbjct: 361 APLLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPDLWEDPTAFKPERHGDSSG--AD 420

Query: 421 SCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQP 480
           S KL+PFGLGRRACPGVG+AQRVVGL LASLIQ FEWER S SLVDM EG+GLTMPKAQP
Sbjct: 421 SYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQP 480

Query: 481 LVAKCKPRPIMKAILNAPTGNI 503
           LVAKCKPRPIMKAI+N    NI
Sbjct: 481 LVAKCKPRPIMKAIMNVEIDNI 498

BLAST of Spg031144 vs. NCBI nr
Match: XP_023532689.1 (cytochrome P450 81F3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532690.1 cytochrome P450 81F3-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 773.1 bits (1995), Expect = 1.5e-219
Identity = 383/502 (76.29%), Postives = 435/502 (86.65%), Query Frame = 0

Query: 1   MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
           MDILLLSI + L SLL AFNFL  PHRLNLPPTP FSLPLIGH HLLKHPLHR+LH LSQ
Sbjct: 1   MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHTLSQ 60

Query: 61  KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
           K+GH+ SLRFGSRLVV++SSPSAV EC +KNDI+LANRPLL+TGK+LAYNHTT+A SPYG
Sbjct: 61  KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120

Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMF 180
           +HWRNLRRI SLEIFST+RLN+F+GIRE+EVKRLL KLC    EE R VELEPML DL F
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGGWLEEGREVELEPMLSDLTF 180

Query: 181 NIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMN 240
           N++M M+ G +F   D+G S+ F++LVTQIMAH G++NPG+FIP+WNW+DPTGF+KRIMN
Sbjct: 181 NVVMGMVGGRRF--YDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMN 240

Query: 241 VGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDT 300
           + +R D  LQ LIDE R+ KD GNTMI  LLCLQK EPEYYSD IIKGLIQVI LAGIDT
Sbjct: 241 LARRGDEVLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDT 300

Query: 301 TAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPA 360
           +AVTLEWALSQLLNNP VLKKA+AEID LIG+ERLVNE+D+P+L YL+GIISETLRLNPA
Sbjct: 301 SAVTLEWALSQLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRLNPA 360

Query: 361 APLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGD 420
           APLL+PHCAF+D KIEGYDVPRDTI+LINAWAIH +PNLWED T F+PERH +++    D
Sbjct: 361 APLLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPNLWEDPTAFKPERHGDTSG--AD 420

Query: 421 SCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQP 480
           S KL+PFGLGRRACPGVG+AQRVVGL LASLIQ FEWER S SLVDM EG+GLTMPKAQP
Sbjct: 421 SYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQP 480

Query: 481 LVAKCKPRPIMKAILNAPTGNI 503
           LVAKCKPRPIMKAI+N    NI
Sbjct: 481 LVAKCKPRPIMKAIMNVEIDNI 498

BLAST of Spg031144 vs. NCBI nr
Match: XP_022149455.1 (cytochrome P450 81E8-like [Momordica charantia])

HSP 1 Score: 766.9 bits (1979), Expect = 1.1e-217
Identity = 378/496 (76.21%), Postives = 429/496 (86.49%), Query Frame = 0

Query: 1   MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
           MDIL LSIS+ LFSLLLAF+FL R  R NLPP+P FSLP++GHLHL+KHP+H+T H+LS 
Sbjct: 1   MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60

Query: 61  KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
           KYGH+FSLRFGSR+VV+VSSPSAV EC +KNDIVLANRPLL +GK LAYNHTTMA +PYG
Sbjct: 61  KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120

Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMF 180
           +HWRNLRRI +LEIFST+RLN+FLGIRE EV+ L+ KLC +S+EEF  VELEPMLFDL F
Sbjct: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180

Query: 181 NIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMN 240
           NI+MRM+ G K+  E    S+ F+E+VTQ++AH G  NPG+FIPIWNWIDPTGFEKRI+ 
Sbjct: 181 NIVMRMVAGKKYYEEQ--QSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIK 240

Query: 241 VGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDT 300
           +GQR+D  LQ LIDEIRN++   NTMID LLCLQK EPEYYSDQIIKGLIQVILLAGIDT
Sbjct: 241 LGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDT 300

Query: 301 TAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPA 360
            AVTLEWALS+LLNNP+VL+KAKAEIDC +GQERLVNEAD+ SL YL+GII ETLRLNPA
Sbjct: 301 AAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPA 360

Query: 361 APLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGD 420
           APLL+PHCA  D KI GYDVPRDTIILIN WAIH +PNLWEDATNF+PERH N+  +  D
Sbjct: 361 APLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGV--D 420

Query: 421 SCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQP 480
           S KL+PFG GRR+CPGVG+AQRVVGLTLASLIQ FEWER S SLVDM EGKGLTMPKAQ 
Sbjct: 421 SYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQH 480

Query: 481 LVAKCKPRPIMKAILN 497
           LVAKCKPRPIMK ILN
Sbjct: 481 LVAKCKPRPIMKLILN 492

BLAST of Spg031144 vs. NCBI nr
Match: XP_023512186.1 (isoflavone 2'-hydroxylase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512187.1 isoflavone 2'-hydroxylase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 757.3 bits (1954), Expect = 8.4e-215
Identity = 371/495 (74.95%), Postives = 428/495 (86.46%), Query Frame = 0

Query: 1   MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
           MDILLLSIS+F+F LL AFNF+L+PHRLNLPP+P F LP+IGHLHLL HP+HRTL  LSQ
Sbjct: 1   MDILLLSISLFIFPLLFAFNFVLKPHRLNLPPSPLFCLPIIGHLHLLNHPIHRTLTNLSQ 60

Query: 61  KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
           KYGH+FSLRFGSRLVV++SSPSAV EC +KNDIVLANRPLLN+ K++ YNHT M+ SPYG
Sbjct: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPLLNSAKYIDYNHTVMSVSPYG 120

Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEE-FRLVELEPMLFDLM 180
           +HWRNLRRI+SLEIFST+RLNLFLGIRE E+K LLCKLC DS E+ FR+VELEPML DLM
Sbjct: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180

Query: 181 FNIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIM 240
           FNI+ RM+ G +F    D     F+E+VTQIMAH+G  NPG+FIP+W +IDP+GFEKR+ 
Sbjct: 181 FNIVTRMVAGKRFYERHD----KFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVK 240

Query: 241 NVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGID 300
            +G+  D  L+ L+DEIR+K D GNTMI RLL LQ TEPEYYSDQIIKGLIQ ILLAGID
Sbjct: 241 KLGKTCDEILRELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGID 300

Query: 301 TTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNP 360
           T AVTLEWALSQLLN+P+VL+KA+AEID  IG ERLVNE D+P+L+Y++GIISET+RLNP
Sbjct: 301 TLAVTLEWALSQLLNHPEVLEKARAEIDNSIGYERLVNEVDLPNLSYVQGIISETMRLNP 360

Query: 361 AAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQG 420
           AAPLL+PHCAFE+ KIEGYDVPRDTIILINAWA+H +PNLWEDAT+F+PERHAN      
Sbjct: 361 AAPLLVPHCAFEECKIEGYDVPRDTIILINAWAMHRDPNLWEDATSFKPERHANGMKNGA 420

Query: 421 DSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQ 480
           DS KLIPFGLGRRACPGVG+AQRV GLTLASLIQ FEWER + SLVDM EGKG+TMPKAQ
Sbjct: 421 DSYKLIPFGLGRRACPGVGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQ 480

Query: 481 PLVAKCKPRPIMKAI 495
           PLVAKCKPRPIMK +
Sbjct: 481 PLVAKCKPRPIMKLV 491

BLAST of Spg031144 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 3.9e-154
Identity = 267/496 (53.83%), Postives = 361/496 (72.78%), Query Frame = 0

Query: 3   ILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQKY 62
           ++LLSIS+ LF         +   R NLPP+P  +LP+IGHLHL+   LHR+L+ LS+KY
Sbjct: 13  VVLLSISLKLFP--------VSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKY 72

Query: 63  GHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYGDH 122
           G +FSL+ G+RLV++VSSP+A  EC +KNDIV ANRPL   GK++ YN+TTM  SPYG+H
Sbjct: 73  GSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEH 132

Query: 123 WRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMFNI 182
           WRNLRR+A++EIFS   LN FL IRE EVK+LL  L   S ++F  VE++  L +L FN+
Sbjct: 133 WRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNV 192

Query: 183 IMRMMVGNKFNSEDDGNSEG--FKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMN 242
            MRM+ G ++  +D  + E   F+ L+ ++  HAG +NPG+F+P   WID   +EK++  
Sbjct: 193 TMRMVAGKRYFGQDVDSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVSK 252

Query: 243 VGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDT 302
           + Q  DA LQ LI E R  K+   TMID LL LQ+++PEYY+DQIIKG+I V+LLAG DT
Sbjct: 253 ISQEMDAFLQRLIHESRINKN-NVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTDT 312

Query: 303 TAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPA 362
           +AVT+EWA+S LLN+P+ L+KA+ EI+  +G  RL+ E D+P L YL  IISET RL PA
Sbjct: 313 SAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCPA 372

Query: 363 APLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGD 422
           AP+L+PH + +D K++GYDVP+ TI+L+NAWAIH +P  W++ T F+PERH     ++ +
Sbjct: 373 APMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERH---GGVELE 432

Query: 423 SCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQP 482
             KL+PFG+GRR+CPG G+AQRVVGLTL +LIQCFEW+R   + +DM EG GLTMPKAQP
Sbjct: 433 PSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQP 492

Query: 483 LVAKCKPRPIMKAILN 497
           L A CKPR I+  +++
Sbjct: 493 LEALCKPRNILHKVVS 496

BLAST of Spg031144 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 512.3 bits (1318), Expect = 6.2e-144
Identity = 258/501 (51.50%), Postives = 354/501 (70.66%), Query Frame = 0

Query: 1   MDIL-LLSISVFLFSLLLAFNFLLRPHRL-NLPPTPPFSLPLIGHLHLLKHPLHRTLHKL 60
           M+IL LLS SVF  +L   FN ++R  +  NLPP PP SLP+IG+LH LK PLHRT   L
Sbjct: 1   MEILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPP-SLPIIGNLHHLKRPLHRTFKGL 60

Query: 61  SQKYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASP 120
           S+KYGH+FSL FGSRLVV+VSS S   +C +KND+VLANRP   +GK++ YN+TT+ ++ 
Sbjct: 61  SEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTS 120

Query: 121 YGDHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDL 180
           YG+HWRNLRRI +L++ S  R+N F GIR  E +RL+ +L  DS   F  +EL   L+D+
Sbjct: 121 YGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDM 180

Query: 181 MFNIIMRMMVGNKFNSEDDGNSE-----GFKELVTQIMAHAGTANPGNFIPIWNWIDPTG 240
            FN IMRM+ G ++  ED   S+      F+++V++++  +G  N  +F+P+  ++D   
Sbjct: 181 TFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFEN 240

Query: 241 FEKRIMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVI 300
            EKR+ ++  + DA L+GLI+E R KK+  NTMID LL LQ ++PEYY+DQIIKGL   +
Sbjct: 241 LEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAM 300

Query: 301 LLAGIDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISE 360
           LLAG D++AVTLEW++S LLN+P+VLKK K E+D  +GQ+RLV+E+D+P L YL+ +I+E
Sbjct: 301 LLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINE 360

Query: 361 TLRLNPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHAN 420
           TLRL   APLLLPH   ++  I GY VP+DTI+LINAWAIH +P LW +AT F+PER   
Sbjct: 361 TLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEK 420

Query: 421 SNAIQGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGL 480
               +G+  KLI FG+GRRACPG G+A R + +TLA LIQCF+W+  +G  +D+ E  G 
Sbjct: 421 ----KGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGF 480

Query: 481 TMPKAQPLVAKCKPRPIMKAI 495
           T+ K  PL A CK RP++  +
Sbjct: 481 TLTKLVPLKAMCKSRPVINKV 496

BLAST of Spg031144 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 4.4e-142
Identity = 262/503 (52.09%), Postives = 350/503 (69.58%), Query Frame = 0

Query: 3   ILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQKY 62
           ++L SI   +F L+++F F L+P + NLPP+PP  LP+IGHL LLK P+HRTL   S+  
Sbjct: 8   VVLYSIFSLIF-LIISFKF-LKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETL 67

Query: 63  GH-----LFSLRFGSRLVVIVSSPS-AVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAA 122
            H     + SLR GSRLV +VSS   A  EC  KND+VLANRP +  GKH+ YN+T M A
Sbjct: 68  DHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIA 127

Query: 123 SPYGDHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLF 182
           +PYGDHWRNLRR+ ++EIFST RLN FL +R  EV+RL+ +L   +  +  +VEL+PML 
Sbjct: 128 APYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLM 187

Query: 183 DLMFNIIMRMMVGNKFNSE---DDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTG 242
           DL FN IMRMM G ++  E   D+  ++  ++LV  + A+  + N  +++PI      + 
Sbjct: 188 DLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SS 247

Query: 243 FEKRIMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVI 302
           +E R+  +G+  D  LQGLID+ R +++ G TMID LL LQK++ EYY+DQIIKG+I ++
Sbjct: 248 YENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIM 307

Query: 303 LLAGIDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISE 362
           ++AG +T+AVTLEWALS LLN+P V+ KA+ EID  +G +RL+ EAD+  L YL+ I+ E
Sbjct: 308 VIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLE 367

Query: 363 TLRLNPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHAN 422
           TLRL+PA PLL+PH A ED KI  YD+PR T +L+NAWAIH +PN W+D  +F+PER   
Sbjct: 368 TLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK 427

Query: 423 SNAIQGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGL 482
               Q    KL+ FGLGRRACPG G+AQR+VGL L SLIQCFEWER     VDM+EG G 
Sbjct: 428 EEEAQ----KLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGN 487

Query: 483 TMPKAQPLVAKCKPRPIMKAILN 497
           T+PKA PL A CK RP +  I++
Sbjct: 488 TVPKAIPLKAICKARPFLHKIIS 502

BLAST of Spg031144 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 1.9e-140
Identity = 260/499 (52.10%), Postives = 344/499 (68.94%), Query Frame = 0

Query: 5   LLSISVFLFSLLLAFNFLLR--PHRL-NLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQK 64
           L   S+   S ++    LL+    RL NLPP PP ++P+IG+LH LKHPLHRT   LSQ 
Sbjct: 2   LFYYSLLSLSFIITIKILLKITSRRLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQT 61

Query: 65  YGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYGD 124
           YG +FSL FGSRLVV+VSSPS  HEC +KNDI+LANRP   TGK++ YN+TT+ ++ YGD
Sbjct: 62  YGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGD 121

Query: 125 HWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEE---FRLVELEPMLFDL 184
           HWRNLRRI ++++ S +RLN FLG+R  E  RL+ KL  D   E   F  VEL P L ++
Sbjct: 122 HWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEM 181

Query: 185 MFNIIMRMMVGNKFNSEDDGNSE-----GFKELVTQIMAHAGTANPGNFIPIWNWIDPTG 244
            FN +MRM+ G ++  +D   S+      F+E+++++M+  G  N G+F+P+   +D   
Sbjct: 182 TFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDN 241

Query: 245 FEKRIMNVGQRADAQLQGLIDEIR--NKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQ 304
            EKR   + +R++A L+GLI+E R  N   +G TMID LL L +++PEYYSD +IKGLIQ
Sbjct: 242 LEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQ 301

Query: 305 VILLAGIDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGII 364
            +LLAG DT+AVT+EW +S+LLN+P+VLKKAK E+D  IG+ +LV+E D+  L YL+ II
Sbjct: 302 GMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNII 361

Query: 365 SETLRLNPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERH 424
           SETLRL+P APLLLPH + ED  I  ++VP+DTIIL N W IH +P  W DA +F+PER 
Sbjct: 362 SETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERF 421

Query: 425 ANSNAIQGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGK 484
                +     K++ FGLGRRACPG+ +AQR VG T+  LIQCFEWER S   +DM EGK
Sbjct: 422 EKEEEVN----KVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGK 481

Query: 485 GLTMPKAQPLVAKCKPRPI 491
           G+TMP   PL A CK  PI
Sbjct: 482 GITMPMKIPLRAMCKALPI 495

BLAST of Spg031144 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 3.2e-140
Identity = 258/505 (51.09%), Postives = 346/505 (68.51%), Query Frame = 0

Query: 1   MDILLLSISVFLFSLLLAFNFLL--RPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKL 60
           M+ + L +S+F F + L+   L   RP +LNLPP+P    P+IGHLHLLK PLHR    L
Sbjct: 8   METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67

Query: 61  SQKYGH--LFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAA 120
           S+   +  +FSL  GSRLV +VSS +   EC +KND+VLANRP    GKH+ YN TTM  
Sbjct: 68  SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127

Query: 121 SPYGDHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLF 180
           + YGD WRNLRRI ++EIFS+ RLN F+ IR+ E++RL+  L  +S   F  VE++P+  
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFM 187

Query: 181 DLMFNIIMRMMVGNKF---NSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTG 240
            L  N I+RM+ G +F    +E+D  ++  ++L+ +++   G  N  ++ PI  ++  T 
Sbjct: 188 CLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TN 247

Query: 241 FEKRIMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVI 300
           +EK +  +  R D  LQ L++E R +K +GNTMID LL LQ+T+P+YY+D IIKG+I V+
Sbjct: 248 YEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVM 307

Query: 301 LLAGIDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISE 360
           +LAG DT+A TLEWA+S LLN+P+VL+KAK EID  IG +RLV E DI  L YL+ I+SE
Sbjct: 308 ILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSE 367

Query: 361 TLRLNPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHAN 420
           TLRL P AP+LLPH A ED  ++GYDVPR TIIL+NAWAIH +P LWE+   F+PER   
Sbjct: 368 TLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEK 427

Query: 421 SNAIQGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREG-KG 480
               +G+  KL+PFG+GRR+CPG G+AQR+V L L SL+QCFEWER     +DMRE  KG
Sbjct: 428 ----KGEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKG 487

Query: 481 LTMPKAQPLVAKCKPRPIMKAILNA 498
            TM KA  L A CK RPI+  +L+A
Sbjct: 488 TTMRKATSLQAMCKARPIVHKVLDA 506

BLAST of Spg031144 vs. ExPASy TrEMBL
Match: A0A6J1L2W8 (cytochrome P450 81F3-like OS=Cucurbita maxima OX=3661 GN=LOC111499919 PE=3 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 1.1e-220
Identity = 384/502 (76.49%), Postives = 436/502 (86.85%), Query Frame = 0

Query: 1   MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
           MDILLLSI + L SLL AFNFL  PHRLNLPPTP FSLPLIGH HLLKHPLHR+LH LSQ
Sbjct: 1   MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHNLSQ 60

Query: 61  KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
           K+GH+ SLRFGSRLVV++SSPSAV EC +KNDI+LANRPLL+TGK+LAYNHTT+A SPYG
Sbjct: 61  KHGHVISLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120

Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMF 180
           +HWRNLRRI SLEIFSTSRLNLFLGIR++EVKRLL KLC D  EEFR VE+EPML DL F
Sbjct: 121 EHWRNLRRICSLEIFSTSRLNLFLGIRQNEVKRLLRKLCGDWLEEFREVEVEPMLSDLTF 180

Query: 181 NIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMN 240
           N++M M+ G +F   D+G S+ F++LVTQIMAH G++NPG+FIP+WNW+DPTGF+KRIMN
Sbjct: 181 NVVMGMVGGRRF--YDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMN 240

Query: 241 VGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDT 300
           + +R D  LQ LIDE R+ KD GN+MI  LLCLQK EPEYYSD IIKGLIQVI LAGIDT
Sbjct: 241 LARRGDELLQELIDEARDGKDGGNSMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDT 300

Query: 301 TAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPA 360
           +AVTLEWALS LLNNP VLKKA+AEID LIG+ERLVNE+D+P+L YL+GIISETLRLNPA
Sbjct: 301 SAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLRYLQGIISETLRLNPA 360

Query: 361 APLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGD 420
           APLL+PHCAF+D KIEGYDVPRDTI+LINAWAIH +P+LWED T F+PERH +S+    D
Sbjct: 361 APLLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPDLWEDPTAFKPERHGDSSG--AD 420

Query: 421 SCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQP 480
           S KL+PFGLGRRACPGVG+AQRVVGL LASLIQ FEWER S SLVDM EG+GLTMPKAQP
Sbjct: 421 SYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQP 480

Query: 481 LVAKCKPRPIMKAILNAPTGNI 503
           LVAKCKPRPIMKAI+N    NI
Sbjct: 481 LVAKCKPRPIMKAIMNVEIDNI 498

BLAST of Spg031144 vs. ExPASy TrEMBL
Match: A0A6J1G5P7 (cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 1.1e-220
Identity = 383/502 (76.29%), Postives = 435/502 (86.65%), Query Frame = 0

Query: 1   MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
           MDILLLSI + L SLL AFNFL  PHRLNLPPTP FSLPLIGH HLLKHPLHR+LH LSQ
Sbjct: 1   MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQ 60

Query: 61  KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
           K+GH+ SLRFGSRLVV++SSPSAV EC +KNDI+LANRPLL+TGK+LAYNHTT+A SPYG
Sbjct: 61  KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYG 120

Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMF 180
           +HWRNLRRI SLEIFST+RLN+F+GIRE+EVKRLL KLC D  EEFR VELEPML DL F
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSEEFREVELEPMLSDLTF 180

Query: 181 NIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMN 240
           N++M M+ G +F   D+G S+ F++LVTQIMAH G++NPG+FIP+WNW+DPTGF+KRIMN
Sbjct: 181 NVVMGMVGGRRF--YDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMN 240

Query: 241 VGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDT 300
           + +R D  LQ LIDE R+ KD GNTMI  LLCLQK EPEYYSD IIKGLIQVI LAGIDT
Sbjct: 241 LARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDT 300

Query: 301 TAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPA 360
           +AVTLEWALS LLNNP VLKKA+AEID LIG+ERLVNE+D+P+L YL+GIISETLRLNPA
Sbjct: 301 SAVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRLNPA 360

Query: 361 APLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGD 420
           APLL+PHCAF+D KIEGYDVPRDTI+LIN WAIH +PNLWED T F+PERH +++    D
Sbjct: 361 APLLVPHCAFQDCKIEGYDVPRDTIVLINTWAIHRDPNLWEDPTAFKPERHGDTSG--AD 420

Query: 421 SCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQP 480
           S KL+PFGLGRRACPGVG+AQRVVGL LASLIQ FEWER S SLVDM EG+GLTMPKAQP
Sbjct: 421 SYKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQP 480

Query: 481 LVAKCKPRPIMKAILNAPTGNI 503
           LVAKCKPRPIMKAI+N    NI
Sbjct: 481 LVAKCKPRPIMKAIMNVEIDNI 498

BLAST of Spg031144 vs. ExPASy TrEMBL
Match: A0A6J1D5S1 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 5.2e-218
Identity = 378/496 (76.21%), Postives = 429/496 (86.49%), Query Frame = 0

Query: 1   MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
           MDIL LSIS+ LFSLLLAF+FL R  R NLPP+P FSLP++GHLHL+KHP+H+T H+LS 
Sbjct: 1   MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60

Query: 61  KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
           KYGH+FSLRFGSR+VV+VSSPSAV EC +KNDIVLANRPLL +GK LAYNHTTMA +PYG
Sbjct: 61  KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120

Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMF 180
           +HWRNLRRI +LEIFST+RLN+FLGIRE EV+ L+ KLC +S+EEF  VELEPMLFDL F
Sbjct: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180

Query: 181 NIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIMN 240
           NI+MRM+ G K+  E    S+ F+E+VTQ++AH G  NPG+FIPIWNWIDPTGFEKRI+ 
Sbjct: 181 NIVMRMVAGKKYYEEQ--QSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIK 240

Query: 241 VGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGIDT 300
           +GQR+D  LQ LIDEIRN++   NTMID LLCLQK EPEYYSDQIIKGLIQVILLAGIDT
Sbjct: 241 LGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDT 300

Query: 301 TAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNPA 360
            AVTLEWALS+LLNNP+VL+KAKAEIDC +GQERLVNEAD+ SL YL+GII ETLRLNPA
Sbjct: 301 AAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPA 360

Query: 361 APLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQGD 420
           APLL+PHCA  D KI GYDVPRDTIILIN WAIH +PNLWEDATNF+PERH N+  +  D
Sbjct: 361 APLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGV--D 420

Query: 421 SCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQP 480
           S KL+PFG GRR+CPGVG+AQRVVGLTLASLIQ FEWER S SLVDM EGKGLTMPKAQ 
Sbjct: 421 SYKLLPFGSGRRSCPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQH 480

Query: 481 LVAKCKPRPIMKAILN 497
           LVAKCKPRPIMK ILN
Sbjct: 481 LVAKCKPRPIMKLILN 492

BLAST of Spg031144 vs. ExPASy TrEMBL
Match: A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 1.6e-214
Identity = 369/498 (74.10%), Postives = 435/498 (87.35%), Query Frame = 0

Query: 1   MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
           MDILLLSIS+ LFSL + F+FLLRP+R NLPP+P F LPLIGHLHL+KHP+HRTLHKLS 
Sbjct: 81  MDILLLSISLSLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSH 140

Query: 61  KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
            YGH+FSLR+GSRLVV+VSSPSAV EC +KNDIVLANRPLL +GK LAYNHTT+A SPYG
Sbjct: 141 NYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYG 200

Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMF 180
           +HWRNLRRI++LEIFSTSRLNLFLGIRE EVK LL KL  DS +EFR VEL+ ML D  F
Sbjct: 201 EHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTF 260

Query: 181 NIIMRMMVGNKFNSE---DDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKR 240
           NI++RM+ G K+  E   D+G S+ F+E+V +I+A+ GTAN G+F+P+WNWIDPTG+EKR
Sbjct: 261 NIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKR 320

Query: 241 IMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAG 300
           IM +G+R+D  LQ L+DEIRN+KD GNTMID LL LQ T+PEY+SDQIIKGL Q++LLAG
Sbjct: 321 IMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAG 380

Query: 301 IDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRL 360
           IDT A+TLEWALSQLLNNP+VLKKA+AEID  IG++RLV+E D+ SL+YL+G+I+ETLRL
Sbjct: 381 IDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRL 440

Query: 361 NPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAI 420
           NPAAPLL+PHCA ED KIEGYD+PRDTIILINAWAIH + N+WEDAT+F+PERHAN+   
Sbjct: 441 NPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMG- 500

Query: 421 QGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPK 480
             DS KL+PFGLGRRACPG+G+AQRVVGLTLASLIQCFEWER + SLVDM EG+GLTMPK
Sbjct: 501 GVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPK 560

Query: 481 AQPLVAKCKPRPIMKAIL 496
           AQPLVAKCKPRPI+KAIL
Sbjct: 561 AQPLVAKCKPRPILKAIL 577

BLAST of Spg031144 vs. ExPASy TrEMBL
Match: A0A6J1FXS5 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448183 PE=3 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 1.3e-213
Identity = 371/495 (74.95%), Postives = 427/495 (86.26%), Query Frame = 0

Query: 1   MDILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQ 60
           MDILLLSIS+FLF+LL AFNF+L+PHRLNLPP P   LPLIGHLHLL HP+HRTL  LSQ
Sbjct: 1   MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60

Query: 61  KYGHLFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYG 120
           KYGH+FSLRFGSRLVV++SSPSAV EC +KNDI LANRPLLN+GK+L YNHT M+ SPYG
Sbjct: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 120

Query: 121 DHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEE-FRLVELEPMLFDLM 180
           +HWRNLRRI SLEIFST+RLNLFLG+R+ E+K LLCKLC DS E+ FR+VELEPML DLM
Sbjct: 121 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180

Query: 181 FNIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRIM 240
           FNI+M+M+ G K     +G+S  FKE+VTQIMAH+G  NP +FIP+W +IDPTGF KR  
Sbjct: 181 FNIVMKMVAGKK---SFEGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGK 240

Query: 241 NVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGID 300
            + + +D  LQ L+DEIR+K+D GNTMI RLL LQ TEPEYYSDQIIKGLIQ ILLAGID
Sbjct: 241 KLAKTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGID 300

Query: 301 TTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLNP 360
           T AVTLEWA+SQLLNNP+VL+KA+AEID  IG ERLVNE+D+PSL+Y++GIISETLRL+P
Sbjct: 301 TIAVTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHP 360

Query: 361 AAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQG 420
           AAPLLLPHCAFE+ KIEGYDVPRDTII INAWA+H +PNLWEDAT+F+PERHAN      
Sbjct: 361 AAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGA 420

Query: 421 DSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKAQ 480
           +S KLIPFGLGRRACPG+G+AQRV GLTLASLIQ FEWER + SLVDM EGKG+TMPKAQ
Sbjct: 421 ESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQ 480

Query: 481 PLVAKCKPRPIMKAI 495
           PLVAKCKPRPIMK +
Sbjct: 481 PLVAKCKPRPIMKLV 492

BLAST of Spg031144 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 506.1 bits (1302), Expect = 3.2e-143
Identity = 262/503 (52.09%), Postives = 350/503 (69.58%), Query Frame = 0

Query: 3   ILLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQKY 62
           ++L SI   +F L+++F F L+P + NLPP+PP  LP+IGHL LLK P+HRTL   S+  
Sbjct: 8   VVLYSIFSLIF-LIISFKF-LKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETL 67

Query: 63  GH-----LFSLRFGSRLVVIVSSPS-AVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAA 122
            H     + SLR GSRLV +VSS   A  EC  KND+VLANRP +  GKH+ YN+T M A
Sbjct: 68  DHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIA 127

Query: 123 SPYGDHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLF 182
           +PYGDHWRNLRR+ ++EIFST RLN FL +R  EV+RL+ +L   +  +  +VEL+PML 
Sbjct: 128 APYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLM 187

Query: 183 DLMFNIIMRMMVGNKFNSE---DDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTG 242
           DL FN IMRMM G ++  E   D+  ++  ++LV  + A+  + N  +++PI      + 
Sbjct: 188 DLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SS 247

Query: 243 FEKRIMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVI 302
           +E R+  +G+  D  LQGLID+ R +++ G TMID LL LQK++ EYY+DQIIKG+I ++
Sbjct: 248 YENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIM 307

Query: 303 LLAGIDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISE 362
           ++AG +T+AVTLEWALS LLN+P V+ KA+ EID  +G +RL+ EAD+  L YL+ I+ E
Sbjct: 308 VIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLE 367

Query: 363 TLRLNPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHAN 422
           TLRL+PA PLL+PH A ED KI  YD+PR T +L+NAWAIH +PN W+D  +F+PER   
Sbjct: 368 TLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK 427

Query: 423 SNAIQGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGL 482
               Q    KL+ FGLGRRACPG G+AQR+VGL L SLIQCFEWER     VDM+EG G 
Sbjct: 428 EEEAQ----KLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGN 487

Query: 483 TMPKAQPLVAKCKPRPIMKAILN 497
           T+PKA PL A CK RP +  I++
Sbjct: 488 TVPKAIPLKAICKARPFLHKIIS 502

BLAST of Spg031144 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 503.1 bits (1294), Expect = 2.7e-142
Identity = 260/498 (52.21%), Postives = 353/498 (70.88%), Query Frame = 0

Query: 4   LLLSISVFLFSLLLAFNFLLRPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLSQKYG 63
           L+ SI   + SL+     L R  + NLPP+P +SLP+IGHL LLK P+HRT   LSQ   
Sbjct: 6   LIFSILFVVLSLIYLIGKLKR--KPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLN 65

Query: 64  H--LFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAASPYGD 123
           +  +FSLR G+RLV + SS S   EC +KND+VLANRP     KH+AY++TTM A+ YGD
Sbjct: 66  NAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGD 125

Query: 124 HWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFDLMFN 183
           HWRNLRRI S+EIFS  RLN FL IR+ E++RL+ +L  +  +EF  V+++ ML DL FN
Sbjct: 126 HWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFN 185

Query: 184 IIMRMMVGNKF---NSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKRI 243
            I+RM+ G ++     EDD  ++  ++L+  ++A AG  N  +++P+   +  + +E R+
Sbjct: 186 NILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRV 245

Query: 244 MNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAGI 303
             +  R D  LQGL+DE R  K++GNTMID LL LQ+++P+Y++D+IIKG +  ++LAG 
Sbjct: 246 KKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGT 305

Query: 304 DTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRLN 363
           DT+AVTLEWALS +LN+P VL KA+ EID  IG +RL++E+DI +L YL+ I+SETLRL 
Sbjct: 306 DTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLY 365

Query: 364 PAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAIQ 423
           PAAP+LLPH A ED K+ GYD+PR TI+L N WAIH +P LW+D  +F+PER       +
Sbjct: 366 PAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEK----E 425

Query: 424 GDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPKA 483
           G++ KL+PFGLGRRACPG G+A R++ LTL SLIQC EWE+  G  VDM EGKG+TMPKA
Sbjct: 426 GEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKI-GEEVDMSEGKGVTMPKA 485

Query: 484 QPLVAKCKPRPIMKAILN 497
           +PL A C+ RP +  I N
Sbjct: 486 KPLEAMCRARPSVVKIFN 494

BLAST of Spg031144 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 501.5 bits (1290), Expect = 7.8e-142
Identity = 255/498 (51.20%), Postives = 346/498 (69.48%), Query Frame = 0

Query: 1   MDILLLSISVFLFSLLLAFNFLLRPHR-LNLPPTPPFSLPLIGHLHLLKHPLHRTLHKLS 60
           MD+  + I  FL    LA   L R  R LNLPP+P  SLP+IGHLHLLK PLHRT   LS
Sbjct: 1   MDLNQILILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLS 60

Query: 61  QKYGH--LFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAAS 120
           +  G+  +F LR G+RLV ++SS S   EC +KND+VLANRP     KHL YN T + ++
Sbjct: 61  KSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSA 120

Query: 121 PYGDHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLFD 180
            YGDHWRNLRRIA++EIFST RLN FL IR+ E++RL+  L  DS   F  VE++ +L +
Sbjct: 121 SYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTN 180

Query: 181 LMFNIIMRMMVGNKFNSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTGFEKR 240
           L  N  +RM+ G ++  ED+ +++  K LV++ +  AG  NP +++ I  W+  + +EKR
Sbjct: 181 LASNTTIRMLAGKRYFGEDNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKR 240

Query: 241 IMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVILLAG 300
           I N+G R D  LQ L+DE R +K++G TMID LL LQ  +P+YY+D IIKG+I  +++AG
Sbjct: 241 IKNLGNRFDTFLQKLVDEKRAEKEKGETMIDHLLALQDIQPDYYTDVIIKGIILTLIIAG 300

Query: 301 IDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISETLRL 360
            DT++VTLEWA+S LLN+P++LKKA+ EID  +G +RLV+E+DI +L+YL+ I+ ETLR+
Sbjct: 301 TDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLRM 360

Query: 361 NPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHANSNAI 420
            PA PLLLPH + ED K+ GYD+P  T++L NAWA+H +P +WED   F+PER       
Sbjct: 361 YPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEK---- 420

Query: 421 QGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGLTMPK 480
           +G++ KLI FG+GRRACPG G+A R++   L SL+QCFEWER     VDM E KG T+PK
Sbjct: 421 EGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPK 480

Query: 481 AQPLVAKCKPRPIMKAIL 496
           A PL A CK R I+  ++
Sbjct: 481 AIPLRAMCKARSIVDKLI 492

BLAST of Spg031144 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 500.0 bits (1286), Expect = 2.3e-141
Identity = 258/505 (51.09%), Postives = 346/505 (68.51%), Query Frame = 0

Query: 1   MDILLLSISVFLFSLLLAFNFLL--RPHRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKL 60
           M+ + L +S+F F + L+   L   RP +LNLPP+P    P+IGHLHLLK PLHR    L
Sbjct: 8   METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67

Query: 61  SQKYGH--LFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAA 120
           S+   +  +FSL  GSRLV +VSS +   EC +KND+VLANRP    GKH+ YN TTM  
Sbjct: 68  SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127

Query: 121 SPYGDHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLF 180
           + YGD WRNLRRI ++EIFS+ RLN F+ IR+ E++RL+  L  +S   F  VE++P+  
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFM 187

Query: 181 DLMFNIIMRMMVGNKF---NSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTG 240
            L  N I+RM+ G +F    +E+D  ++  ++L+ +++   G  N  ++ PI  ++  T 
Sbjct: 188 CLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TN 247

Query: 241 FEKRIMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVI 300
           +EK +  +  R D  LQ L++E R +K +GNTMID LL LQ+T+P+YY+D IIKG+I V+
Sbjct: 248 YEKHVKKLAGRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVM 307

Query: 301 LLAGIDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISE 360
           +LAG DT+A TLEWA+S LLN+P+VL+KAK EID  IG +RLV E DI  L YL+ I+SE
Sbjct: 308 ILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSE 367

Query: 361 TLRLNPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHAN 420
           TLRL P AP+LLPH A ED  ++GYDVPR TIIL+NAWAIH +P LWE+   F+PER   
Sbjct: 368 TLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEK 427

Query: 421 SNAIQGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREG-KG 480
               +G+  KL+PFG+GRR+CPG G+AQR+V L L SL+QCFEWER     +DMRE  KG
Sbjct: 428 ----KGEDKKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKG 487

Query: 481 LTMPKAQPLVAKCKPRPIMKAILNA 498
            TM KA  L A CK RPI+  +L+A
Sbjct: 488 TTMRKATSLQAMCKARPIVHKVLDA 506

BLAST of Spg031144 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 499.6 bits (1285), Expect = 3.0e-141
Identity = 253/498 (50.80%), Postives = 351/498 (70.48%), Query Frame = 0

Query: 1   MDILLLSISVFLFSLLLAFNFLLRP--HRLNLPPTPPFSLPLIGHLHLLKHPLHRTLHKL 60
           MD+  + +  FLF L ++   LL     + NLPP+P + LP+IGHLHLLK P+HRT H +
Sbjct: 1   MDLTQILLLSFLF-LTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSI 60

Query: 61  SQKYGH--LFSLRFGSRLVVIVSSPSAVHECLSKNDIVLANRPLLNTGKHLAYNHTTMAA 120
           S+  G+  +F LR G+RLV ++SS S   EC +KND+VLANRP +   KH+ YN T M A
Sbjct: 61  SKSLGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIA 120

Query: 121 SPYGDHWRNLRRIASLEIFSTSRLNLFLGIREHEVKRLLCKLCHDSWEEFRLVELEPMLF 180
           + YGDHWRNLRRIA++EIFS+ R++ F  IR+ E++RL+  L  DS   F  VEL+ +L 
Sbjct: 121 ASYGDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLT 180

Query: 181 DLMFNIIMRMMVGNKF---NSEDDGNSEGFKELVTQIMAHAGTANPGNFIPIWNWIDPTG 240
           +L FN I+ M+ G ++    +ED+  ++  +EL+ +IMA AG+ N  +++P  NW+  T 
Sbjct: 181 NLAFNNIIMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TN 240

Query: 241 FEKRIMNVGQRADAQLQGLIDEIRNKKDEGNTMIDRLLCLQKTEPEYYSDQIIKGLIQVI 300
           FE +   +G R D  LQ L+DE R +K++G T+ID LL  Q+TEPEYY+D IIKG+I  +
Sbjct: 241 FENQTKILGNRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILAL 300

Query: 301 LLAGIDTTAVTLEWALSQLLNNPQVLKKAKAEIDCLIGQERLVNEADIPSLNYLRGIISE 360
           +LAG DT++VTLEWA+S LLN+P++L+KA+AEID  IG +RLV E+DI +L+YL+ I+SE
Sbjct: 301 VLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSE 360

Query: 361 TLRLNPAAPLLLPHCAFEDSKIEGYDVPRDTIILINAWAIHTNPNLWEDATNFEPERHAN 420
           TLRL PA PLLLPH + ++ K+ GYD+PR T++L N WA+H +P LWE+   F+PER   
Sbjct: 361 TLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERFEK 420

Query: 421 SNAIQGDSCKLIPFGLGRRACPGVGVAQRVVGLTLASLIQCFEWERASGSLVDMREGKGL 480
               +G++ KL+PFG+GRRACPG  + +R+V L L  LIQ FEWER    LVDM EG+G+
Sbjct: 421 ----EGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGI 480

Query: 481 TMPKAQPLVAKCKPRPIM 492
           TMPKA PL A CK R I+
Sbjct: 481 TMPKATPLRAMCKARAIV 491

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022947035.12.3e-22076.29cytochrome P450 81F3 [Cucurbita moschata][more]
XP_023007415.12.3e-22076.49cytochrome P450 81F3-like [Cucurbita maxima][more]
XP_023532689.11.5e-21976.29cytochrome P450 81F3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532690.... [more]
XP_022149455.11.1e-21776.21cytochrome P450 81E8-like [Momordica charantia][more]
XP_023512186.18.4e-21574.95isoflavone 2'-hydroxylase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_02351... [more]
Match NameE-valueIdentityDescription
W8JMU73.9e-15453.83Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1[more]
P931476.2e-14451.50Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2[more]
Q9FG654.4e-14252.09Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1[more]
Q6WNQ91.9e-14052.10Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... [more]
Q9LHA13.2e-14051.09Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1L2W81.1e-22076.49cytochrome P450 81F3-like OS=Cucurbita maxima OX=3661 GN=LOC111499919 PE=3 SV=1[more]
A0A6J1G5P71.1e-22076.29cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1[more]
A0A6J1D5S15.2e-21876.21cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV... [more]
A0A6J1D5S61.6e-21474.10isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE... [more]
A0A6J1FXS51.3e-21374.95isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448183 PE=... [more]
Match NameE-valueIdentityDescription
AT5G36220.13.2e-14352.09cytochrome p450 81d1 [more]
AT4G37370.12.7e-14252.21cytochrome P450, family 81, subfamily D, polypeptide 8 [more]
AT4G37330.17.8e-14251.20cytochrome P450, family 81, subfamily D, polypeptide 4 [more]
AT3G28740.12.3e-14151.09Cytochrome P450 superfamily protein [more]
AT4G37320.13.0e-14150.80cytochrome P450, family 81, subfamily D, polypeptide 5 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 83..104
score: 24.38
coord: 59..78
score: 38.35
coord: 178..196
score: 27.12
coord: 348..366
score: 35.69
coord: 389..413
score: 27.26
coord: 285..302
score: 27.92
coord: 305..331
score: 38.48
coord: 434..457
score: 33.82
coord: 424..434
score: 55.18
IPR001128Cytochrome P450PRINTSPR00385P450coord: 425..434
score: 58.51
coord: 349..360
score: 38.98
coord: 296..313
score: 43.33
IPR001128Cytochrome P450PFAMPF00067p450coord: 32..478
e-value: 3.3E-94
score: 316.2
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 8..490
e-value: 5.5E-119
score: 399.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 31..488
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 8..494
NoneNo IPR availablePANTHERPTHR24298:SF836CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATEDcoord: 8..494
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 427..436

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg031144.1Spg031144.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen