Spg031040 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg031040 vs. NCBI nr
Match: KAA0036941.1 (girdin-like [Cucumis melo var. makuwa])

HSP 1 Score: 458.0 bits (1177), Expect = 1.7e-124
Identity = 236/514 (45.91%), Postives = 336/514 (65.37%), Query Frame = 0

Query: 62  LKAIWEAVHLKEVQKNVKVKGVEESISADYLIERARKHINEEKGLVLLALCIYGMVLYPR 121
           L    E VH  E+QK +KVKG EE++  DYLI+  + +I+E+KGL LLALCIYG V++P+
Sbjct: 118 LSKFLETVHATEIQKYIKVKGGEENVPFDYLIKMTQTYIDEDKGLTLLALCIYGAVIFPK 177

Query: 122 VKGYVDGDVLKLFFSIEQGVDPAIPLLAETVRALNYCKSNKGGKLRCCVPLLYIWVHSHF 181
            +GYVD  V+KLFF +E+GV+P IP+LAET R+LNYC++   GKL CCVPLLYIW+HSH 
Sbjct: 178 AEGYVDRKVIKLFFQMERGVNPIIPILAETFRSLNYCRNKGEGKLNCCVPLLYIWIHSHI 237

Query: 182 KFPEDFGCPKINFSSTWNLTRSTIAEFGTAPWNSSFPRKEAWVSFFSNSKLEHVIWKAHW 241
           KFP +F CP+++FSS WNL R+TI+EFG A W+ ++PRKEAW+SFF+    E+VIWKA W
Sbjct: 238 KFPAEFRCPRLDFSSPWNLMRNTISEFGMAVWDPTYPRKEAWLSFFAKLTSENVIWKAQW 297

Query: 242 MPLKALIYRCGSFHTVPLVGPWGCVHYAPLLALRQSWFKQFIPATHGLHKLEFSYSCEDS 301
           MPLKA+IYRCG FH+VPL+GPWG V+Y PLL LRQ W KQFIP TH L + +FSY  ED 
Sbjct: 298 MPLKAVIYRCGDFHSVPLLGPWGGVNYTPLLVLRQVWLKQFIPPTHNLQESDFSYDPEDC 357

Query: 302 NEKKRQVLTSWRTVRRINGNSHSEGVTPEYLQWRIKRSK-IPITTRDNVGESSSRAVDKP 361
             KKRQ + +W+++R+I    H EGVT  Y  W+  R K I   +R+ V      + ++P
Sbjct: 358 QGKKRQAVCAWKSIRKIKDKGHYEGVTSGYEAWQANRRKNIIDISREVVESGKETSFEQP 417

Query: 362 NQLATERKELVGRNQTLKLENEKLQQEVKRLMNQENRRLIKENHALRNENAALRRATRSQ 421
           NQ   +  EL  +N+ L+ ENEKL++E  + M+            L+NE    +   ++Q
Sbjct: 418 NQWIEKSIELEEKNRLLEQENEKLRKETSQWMDHAT--------YLQNELEKTKSFLKNQ 477

Query: 422 ENMIKDLSKGKETLLELVAELNETINKQKTQLIEFEEANTALRRTLDNLRVNMQAQSEES 481
           + + KDL    ETL E +  +N+     K +        T L+ T+  L + M  +SEE 
Sbjct: 478 DKLEKDL----ETLDEEMRRMNKANRSLKNE-------KTTLQATV-GLHLKMAERSEEY 537

Query: 482 KAFKSYSSSLERQLLAFQRASEQLSLERGQLEEKYSSLRGDYAIMREDMQIILGKVNQTM 541
           +  K+Y+ SL  QL A Q +S++++ E   L   Y  ++ DY +   D Q+++ +V+QT+
Sbjct: 538 EILKNYADSLHYQLTALQNSSKRITQEYESLNTDYVQMKVDYDLHTRDFQVLVERVDQTI 597

Query: 542 NTIKIMARRARGFAEWARDLRENTSPMASNAEEL 575
             ++++++RA GFAEWA DLR N   M  +A++L
Sbjct: 598 EFLRMVSKRADGFAEWAVDLRVNFFSMQPHADDL 611

BLAST of Spg031040 vs. NCBI nr
Match: KAA0036949.1 (girdin-like [Cucumis melo var. makuwa])

HSP 1 Score: 434.5 bits (1116), Expect = 2.0e-117
Identity = 230/524 (43.89%), Postives = 333/524 (63.55%), Query Frame = 0

Query: 62  LKAIWEAVHLKEVQKNVKVKGVEESISADYLIERARKHINEEKGLVLLALCIYGMVLYPR 121
           L    E VH  E+QK +K KG EE++  DYLI+  + +I+E+KGL LLALCIYG V++P+
Sbjct: 89  LSKFLETVHATEIQKYIKAKGGEENVPFDYLIKMTQTYIDEDKGLTLLALCIYGAVIFPK 148

Query: 122 VKGYVDGDVLKLFFSIEQGVDPAIPLLAETVRALNYCKSNKG---------GKLRCCVPL 181
            +GYVD  V+KLFF +E+GV+P IP+LAET R+LNYC+ NKG         GKL CCVPL
Sbjct: 149 AEGYVDRKVIKLFFQMERGVNPIIPILAETFRSLNYCR-NKGEGKLNCCVRGKLNCCVPL 208

Query: 182 LYIWVHSHFKFPEDFGCPKINFSSTWNLTRSTIAEFGTAPWNSSFPRKEAWVSFFSNSKL 241
           LYIW+HSH KFP +F CP+++FSS WNL R+TI+EFG A W+ ++PRKEAW+SFF+    
Sbjct: 209 LYIWIHSHIKFPAEFRCPRLDFSSPWNLMRNTISEFGMAVWDPTYPRKEAWLSFFAKLTS 268

Query: 242 EHVIWKAHWMPLKALIYRCGSFHTVPLVGPWGCVHYAPLLALRQSWFKQFIPATHGLHKL 301
           E+VIWKA WMPLKA+IYRCG FH VPL+GPWG V+  PLL LRQ W KQFIP TH L + 
Sbjct: 269 ENVIWKAQWMPLKAVIYRCGDFHNVPLLGPWGGVNCTPLLVLRQVWLKQFIPPTHNLQES 328

Query: 302 EFSYSCEDSNEKKRQVLTSWRTVRRINGNSHSEGVTPEYLQWRIKRSKIPITTR--DNVG 361
           +FSY  ED   KK   +   +       N           QW  K  ++    R  +   
Sbjct: 329 DFSYDPEDCQGKKTSSIERGKETSFEQPN-----------QWIEKSIELEEKNRLLEQEN 388

Query: 362 ESSSRAVDKPNQLATERKELVGRNQTLKLENEKLQQEVKRLMNQENRRLIKENHALRNEN 421
           E   +   +    AT  +  + + ++     +KL+++++ L ++E RR+ K N +L+NE 
Sbjct: 389 EKLRKETSQWMDHATYLQNKLEKTKSFLKNQDKLEKDLETL-DKEMRRMNKANRSLKNEK 448

Query: 422 AALRRATRSQENMIKDLSKGKETLLELVAELNETINKQKTQLIEFEEANTALRRTLDNLR 481
              +    SQ+  IKDL  GKE  L+LV +LN +I K++T++++ E  N +LR+T+D+L 
Sbjct: 449 TTFQATMGSQDEYIKDLENGKEYFLKLVNDLNTSIRKRETKIMDLEAQNHSLRQTVDSLH 508

Query: 482 VNMQAQSEESKAFKSYSSSLERQLLAFQRASEQLSLERGQLEEKYSSLRGDYAIMREDMQ 541
           + M   SEE +  K+Y+ SL  QL A Q +S++++ E   L   Y  ++ DY +   D Q
Sbjct: 509 LKMVEHSEEYEILKNYADSLHYQLTALQNSSKRITQEYESLNTDYVQMKVDYDMQTRDFQ 568

Query: 542 IILGKVNQTMNTIKIMARRARGFAEWARDLRENTSPMASNAEEL 575
           +++ +V+QT+  ++++++RA  FAE A DLR N   M  +A++L
Sbjct: 569 VLVERVDQTIEFLRMVSKRANDFAESAADLRVNFFSMQPHADDL 599

BLAST of Spg031040 vs. NCBI nr
Match: KAA0036933.1 (uncharacterized protein E6C27_scaffold86G00060 [Cucumis melo var. makuwa])

HSP 1 Score: 398.7 bits (1023), Expect = 1.2e-106
Identity = 218/506 (43.08%), Postives = 307/506 (60.67%), Query Frame = 0

Query: 62  LKAIWEAVHLKEVQKNVKVKGVEESISADYLIERARKHINEEKGLVLLALCIYGMVLYPR 121
           L    E VH  E+QK +K KG EE++  DYLI+  + +I+E+KGL LLALCIYG V++P+
Sbjct: 89  LSKFLETVHATEIQKYIKAKGGEENVPFDYLIKMTQTYIDEDKGLTLLALCIYGAVIFPK 148

Query: 122 VKGYVDGDVLKLFFSIEQGVDPAIPLLAETVRALNYCKSNKG---------GKLRCCVPL 181
            +GYVD  V+KLFF +E+GV+P IP+LAET R+LNYC+ NKG         GKL CCVPL
Sbjct: 149 AEGYVDRKVIKLFFQMERGVNPIIPILAETFRSLNYCR-NKGEGKLNCCVRGKLNCCVPL 208

Query: 182 LYIWVHSHFKFPEDFGCPKINFSSTWNLTRSTIAEFGTAPWNSSFPRKEAWVSFFSNSKL 241
           LYIW+HSH KFP +F CP+++FSS WNL R+TI+EFG A W+ ++PRKEAW+SFF+    
Sbjct: 209 LYIWIHSHIKFPAEFRCPRLDFSSPWNLMRNTISEFGMAVWDPTYPRKEAWLSFFAKLTS 268

Query: 242 EHVIWKAHWMPLKALIYRCGSFHTVPLVGPWGCVHYAPLLALRQSWFKQFIPATHGLHKL 301
           E+VIWKA WMPLKA+IYRCG FH+VPL+GPWG V+Y PLL LRQ W KQFIP TH L   
Sbjct: 269 ENVIWKAQWMPLKAVIYRCGDFHSVPLLGPWGGVNYTPLLVLRQVWLKQFIPPTHNL--- 328

Query: 302 EFSYSCEDSNEKKRQVLTSWRTVRRINGNSHSEGVTPEYLQWRIKRSK-IPITTRDNVGE 361
                                   +I    H EGVT  Y  W+  R K I   +R+ V  
Sbjct: 329 ------------------------KIKDKGHYEGVTSGYEAWQANRRKNIIDISREVVER 388

Query: 362 SSSRAVDKPNQLATERKELVGRNQTLKLENEKLQQEVKRLMNQENRRLIKENHALRNENA 421
               + ++PNQ   +  EL  +N+ L+ ENEKL++E  + M+            L+NE  
Sbjct: 389 GKETSFEQPNQWIEKSIELEQKNRLLEQENEKLRKETSQWMDHAT--------YLQNELE 448

Query: 422 ALRRATRSQENMIKDLSKGKETLLELVAELNETINKQKTQLIEFEEANTALRRTLDNLRV 481
             +   ++Q+ +  DL    ETL + +  +N+     K +        T L+ T+  L +
Sbjct: 449 KTKSFLKNQDKLETDL----ETLDKEMRRMNKANRSMKNE-------KTTLQATV-GLHL 508

Query: 482 NMQAQSEESKAFKSYSSSLERQLLAFQRASEQLSLERGQLEEKYSSLRGDYAIMREDMQI 541
            M  +SEE K  K+Y+  L  QL A Q +S++++ E   L   Y  ++ DY +   D Q+
Sbjct: 509 KMAERSEEYKILKNYADFLHYQLTALQNSSKRITQEYESLNTDYVQMKVDYDLHTRDFQV 546

Query: 542 ILGKVNQTMNTIKIMARRARGFAEWA 558
           ++ +V+QT+  ++++++RA GFAEWA
Sbjct: 569 LVERVDQTIEFLRMVSKRANGFAEWA 546

BLAST of Spg031040 vs. NCBI nr
Match: KAA0036933.1 (uncharacterized protein E6C27_scaffold86G00060 [Cucumis melo var. makuwa])

HSP 1 Score: 86.3 bits (212), Expect = 1.3e-12
Identity = 51/133 (38.35%), Postives = 74/133 (55.64%), Query Frame = 0

Query: 671  ISGTGGVTRSGKCYT----PDQLLKFGLGYKPNKADMIRVQKREKEWRLARFESREPEYE 730
            I G GG + +    T    P    +FGLGYKP+  D IR+Q+++K+ RL + E RE +  
Sbjct: 1372 IMGGGGYSLNKNLETLLKIPSNDGRFGLGYKPSIYDKIRLQEKKKKKRLVKLEMREFDPS 1431

Query: 731  GKVIPHLYHSFESAGIIRPGD--------------FAVAVVNKEEEL-GPWIYPCPENFE 785
             K+IP LY +F+SAGI    D               +VA V +E    G  +Y CP +FE
Sbjct: 1432 IKLIPELYDTFKSAGISYSSDNSDLKDDLLTKMESLSVAAVAQEASFEGNTVYACPPDFE 1491

HSP 2 Score: 378.3 bits (970), Expect = 1.7e-100
Identity = 209/518 (40.35%), Postives = 291/518 (56.18%), Query Frame = 0

Query: 12  SSSSEYDELSTVLLWAEQTQLKHGDNLPYKSPVSSSFSSQVQIIANELGELKAIWEAV-- 71
           S  S++DE S VL WAE+ Q K GD +   S +S     Q+    N+L  LK IWEA+  
Sbjct: 9   SFESKFDESSDVLKWAEEMQQKFGDGINNSSQISVLSRCQLSSTKNDLANLKMIWEALTP 68

Query: 72  -----------HL----------------------------------------------- 131
                      H+                                               
Sbjct: 69  QRRFMFSKKYGHIAELMYKPVNYFALRAIINFWDPAYGCFTFGSCDLLPTIEEYQVMLSM 128

Query: 132 --------------------KEVQKNVKVKGVEESISADYLIERARKHINEEKGLVLLAL 191
                                E+QK +KVK  EE++  DYLI+  + +I+E+KGL LLAL
Sbjct: 129 PEKEREIVYFFNPKQTTKTTTEIQKYIKVKDGEENVPFDYLIKMTQTYIDEDKGLTLLAL 188

Query: 192 CIYGMVLYPRVKGYVDGDVLKLFFSIEQGVDPAIPLLAETVRALNYCKSNKGGKLRCCVP 251
           CIYG V++P+ +GYVDG V+KLFF +E+GV+P IP+LA+T R+LNY ++   GK  CCVP
Sbjct: 189 CIYGAVIFPKAEGYVDGKVIKLFFEMERGVNPIIPILAKTFRSLNYYRNKGEGKFNCCVP 248

Query: 252 LLYIWVHSHFKFPEDFGCPKINFSSTWNLTRSTIAEFGTAPWNSSFPRKEAWVSFFSNSK 311
           LLYIW+HSH KFP +F CP+++FSS WNL R+TI+EFG A W+ ++PRKEAW+SFF+   
Sbjct: 249 LLYIWIHSHIKFPAEFRCPRLDFSSPWNLMRNTISEFGMAVWDPTYPRKEAWLSFFAKLT 308

Query: 312 LEHVIWKAHWMPLKALIYRCGSFHTVPLVGPWGCVHYAPLLALRQSWFKQFIPATHGLHK 371
            E+VIWK  WMPLKA+IYR G+FH+VPL+GPWG V+Y PLL LRQ W KQFIP TH L +
Sbjct: 309 SENVIWKTQWMPLKAVIYRRGNFHSVPLLGPWGGVNYTPLLVLRQVWLKQFIPLTHNLQE 368

Query: 372 LEFSYSCEDSNEKKRQVLTSWRTVRRINGNSHSEGVTPEYLQWRI-KRSKIPITTRDNVG 431
            +FSY+ ED   KKRQ + +W+++R+I    H EGV   Y  W+  KR  I   +R+ V 
Sbjct: 369 SDFSYNPEDCQGKKRQAVCTWKSIRKIKDKGHYEGVISGYEAWKANKRKNIIDISREVVE 428

Query: 432 ESSSRAVDKPNQLATERKELVGRNQTLKLENEKLQQEVKRLMNQENRRLIKENHALRNEN 444
           +    +  +PNQ   +  EL  +N+ L+ ENEKL++E  + M          +HA   +N
Sbjct: 429 KGKETSFTQPNQWIEKSIELEEKNRLLEQENEKLRKETSQWM----------DHATYLQN 488

BLAST of Spg031040 vs. NCBI nr
Match: KAA0060423.1 (uncharacterized protein E6C27_scaffold22G002420 [Cucumis melo var. makuwa])

HSP 1 Score: 373.2 bits (957), Expect = 5.4e-99
Identity = 202/507 (39.84%), Postives = 280/507 (55.23%), Query Frame = 0

Query: 12  SSSSEYDELSTVLLWAEQTQLKHGDNLPYKSPVSSSFSSQVQIIANELGELKAIWEA--- 71
           S  S++DE S VL WAE+ Q K GD++   S +S     Q+    N+L  LK IWEA   
Sbjct: 9   SFESKFDEPSDVLKWAEEMQQKFGDDINNSSQISVLSRCQLSFTQNDLANLKMIWEALTP 68

Query: 72  ------------------------------------------------------------ 131
                                                                       
Sbjct: 69  QRRFMFSKKYGHIVELMYIPVNYFALRAIINFWDPAYGCFTFGSCDLLPTIEEYQAMLSM 128

Query: 132 ---------------------------VHLKEVQKNVKVKGVEESISADYLIERARKHIN 191
                                      VH  E+QK +K+KG EE++  DYLI+  + +++
Sbjct: 129 PEKEREIVYFFNPKQTTKRTLSKFLETVHAAEIQKYIKIKGGEENVPFDYLIKMTQTYVD 188

Query: 192 EEKGLVLLALCIYGMVLYPRVKGYVDGDVLKLFFSIEQGVDPAIPLLAETVRALNYCKSN 251
           E+KGL LLALCIYG V++P+ +GYVDG V+KLFF +E+GV+P IP+LAET R+LNYC++ 
Sbjct: 189 EDKGLTLLALCIYGAVIFPKAEGYVDGKVIKLFFEMERGVNPIIPILAETFRSLNYCRNK 248

Query: 252 KGGKLRCCVPLLYIWVHSHFKFPEDFGCPKINFSSTWNLTRSTIAEFGTAPWNSSFPRKE 311
             GKL CC+PLLYIW+HSH KFP +F CP+++FSS WNL R+TI+EFG A W+ ++PRKE
Sbjct: 249 GEGKLNCCLPLLYIWIHSHIKFPAEFRCPRLDFSSPWNLIRNTISEFGMAVWDPTYPRKE 308

Query: 312 AWVSFFSNSKLEHVIWKAHWMPLKALIYRCGSFHTVPLVGPWGCVHYAPLLALRQSWFKQ 371
           AW+SFF+    ++V+WKA WMPLKA+IYRC  FH++PL+GP G V+Y PLL LRQ W KQ
Sbjct: 309 AWLSFFAKLTSKNVVWKAQWMPLKAVIYRCRDFHSMPLLGPRGGVNYTPLLVLRQVWLKQ 368

Query: 372 FIPATHGLHKLEFSYSCEDSNEKKRQVLTSWRTVRRINGNSHSEGVTPEYLQWRIKRSK- 427
           FIP TH L + +FSY  ED   KKRQ + +W+++R+I    H EGV   Y  W+  R K 
Sbjct: 369 FIPPTHNLQESDFSYDPEDCQGKKRQAVCAWKSIRKIKDKGHYEGVISGYEAWQANRRKN 428

BLAST of Spg031040 vs. ExPASy TrEMBL
Match: A0A5A7T5S7 (Girdin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G00170 PE=4 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 8.0e-125
Identity = 236/514 (45.91%), Postives = 336/514 (65.37%), Query Frame = 0

Query: 62  LKAIWEAVHLKEVQKNVKVKGVEESISADYLIERARKHINEEKGLVLLALCIYGMVLYPR 121
           L    E VH  E+QK +KVKG EE++  DYLI+  + +I+E+KGL LLALCIYG V++P+
Sbjct: 118 LSKFLETVHATEIQKYIKVKGGEENVPFDYLIKMTQTYIDEDKGLTLLALCIYGAVIFPK 177

Query: 122 VKGYVDGDVLKLFFSIEQGVDPAIPLLAETVRALNYCKSNKGGKLRCCVPLLYIWVHSHF 181
            +GYVD  V+KLFF +E+GV+P IP+LAET R+LNYC++   GKL CCVPLLYIW+HSH 
Sbjct: 178 AEGYVDRKVIKLFFQMERGVNPIIPILAETFRSLNYCRNKGEGKLNCCVPLLYIWIHSHI 237

Query: 182 KFPEDFGCPKINFSSTWNLTRSTIAEFGTAPWNSSFPRKEAWVSFFSNSKLEHVIWKAHW 241
           KFP +F CP+++FSS WNL R+TI+EFG A W+ ++PRKEAW+SFF+    E+VIWKA W
Sbjct: 238 KFPAEFRCPRLDFSSPWNLMRNTISEFGMAVWDPTYPRKEAWLSFFAKLTSENVIWKAQW 297

Query: 242 MPLKALIYRCGSFHTVPLVGPWGCVHYAPLLALRQSWFKQFIPATHGLHKLEFSYSCEDS 301
           MPLKA+IYRCG FH+VPL+GPWG V+Y PLL LRQ W KQFIP TH L + +FSY  ED 
Sbjct: 298 MPLKAVIYRCGDFHSVPLLGPWGGVNYTPLLVLRQVWLKQFIPPTHNLQESDFSYDPEDC 357

Query: 302 NEKKRQVLTSWRTVRRINGNSHSEGVTPEYLQWRIKRSK-IPITTRDNVGESSSRAVDKP 361
             KKRQ + +W+++R+I    H EGVT  Y  W+  R K I   +R+ V      + ++P
Sbjct: 358 QGKKRQAVCAWKSIRKIKDKGHYEGVTSGYEAWQANRRKNIIDISREVVESGKETSFEQP 417

Query: 362 NQLATERKELVGRNQTLKLENEKLQQEVKRLMNQENRRLIKENHALRNENAALRRATRSQ 421
           NQ   +  EL  +N+ L+ ENEKL++E  + M+            L+NE    +   ++Q
Sbjct: 418 NQWIEKSIELEEKNRLLEQENEKLRKETSQWMDHAT--------YLQNELEKTKSFLKNQ 477

Query: 422 ENMIKDLSKGKETLLELVAELNETINKQKTQLIEFEEANTALRRTLDNLRVNMQAQSEES 481
           + + KDL    ETL E +  +N+     K +        T L+ T+  L + M  +SEE 
Sbjct: 478 DKLEKDL----ETLDEEMRRMNKANRSLKNE-------KTTLQATV-GLHLKMAERSEEY 537

Query: 482 KAFKSYSSSLERQLLAFQRASEQLSLERGQLEEKYSSLRGDYAIMREDMQIILGKVNQTM 541
           +  K+Y+ SL  QL A Q +S++++ E   L   Y  ++ DY +   D Q+++ +V+QT+
Sbjct: 538 EILKNYADSLHYQLTALQNSSKRITQEYESLNTDYVQMKVDYDLHTRDFQVLVERVDQTI 597

Query: 542 NTIKIMARRARGFAEWARDLRENTSPMASNAEEL 575
             ++++++RA GFAEWA DLR N   M  +A++L
Sbjct: 598 EFLRMVSKRADGFAEWAVDLRVNFFSMQPHADDL 611

BLAST of Spg031040 vs. ExPASy TrEMBL
Match: A0A5A7T6E2 (Girdin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G00290 PE=4 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 9.5e-118
Identity = 230/524 (43.89%), Postives = 333/524 (63.55%), Query Frame = 0

Query: 62  LKAIWEAVHLKEVQKNVKVKGVEESISADYLIERARKHINEEKGLVLLALCIYGMVLYPR 121
           L    E VH  E+QK +K KG EE++  DYLI+  + +I+E+KGL LLALCIYG V++P+
Sbjct: 89  LSKFLETVHATEIQKYIKAKGGEENVPFDYLIKMTQTYIDEDKGLTLLALCIYGAVIFPK 148

Query: 122 VKGYVDGDVLKLFFSIEQGVDPAIPLLAETVRALNYCKSNKG---------GKLRCCVPL 181
            +GYVD  V+KLFF +E+GV+P IP+LAET R+LNYC+ NKG         GKL CCVPL
Sbjct: 149 AEGYVDRKVIKLFFQMERGVNPIIPILAETFRSLNYCR-NKGEGKLNCCVRGKLNCCVPL 208

Query: 182 LYIWVHSHFKFPEDFGCPKINFSSTWNLTRSTIAEFGTAPWNSSFPRKEAWVSFFSNSKL 241
           LYIW+HSH KFP +F CP+++FSS WNL R+TI+EFG A W+ ++PRKEAW+SFF+    
Sbjct: 209 LYIWIHSHIKFPAEFRCPRLDFSSPWNLMRNTISEFGMAVWDPTYPRKEAWLSFFAKLTS 268

Query: 242 EHVIWKAHWMPLKALIYRCGSFHTVPLVGPWGCVHYAPLLALRQSWFKQFIPATHGLHKL 301
           E+VIWKA WMPLKA+IYRCG FH VPL+GPWG V+  PLL LRQ W KQFIP TH L + 
Sbjct: 269 ENVIWKAQWMPLKAVIYRCGDFHNVPLLGPWGGVNCTPLLVLRQVWLKQFIPPTHNLQES 328

Query: 302 EFSYSCEDSNEKKRQVLTSWRTVRRINGNSHSEGVTPEYLQWRIKRSKIPITTR--DNVG 361
           +FSY  ED   KK   +   +       N           QW  K  ++    R  +   
Sbjct: 329 DFSYDPEDCQGKKTSSIERGKETSFEQPN-----------QWIEKSIELEEKNRLLEQEN 388

Query: 362 ESSSRAVDKPNQLATERKELVGRNQTLKLENEKLQQEVKRLMNQENRRLIKENHALRNEN 421
           E   +   +    AT  +  + + ++     +KL+++++ L ++E RR+ K N +L+NE 
Sbjct: 389 EKLRKETSQWMDHATYLQNKLEKTKSFLKNQDKLEKDLETL-DKEMRRMNKANRSLKNEK 448

Query: 422 AALRRATRSQENMIKDLSKGKETLLELVAELNETINKQKTQLIEFEEANTALRRTLDNLR 481
              +    SQ+  IKDL  GKE  L+LV +LN +I K++T++++ E  N +LR+T+D+L 
Sbjct: 449 TTFQATMGSQDEYIKDLENGKEYFLKLVNDLNTSIRKRETKIMDLEAQNHSLRQTVDSLH 508

Query: 482 VNMQAQSEESKAFKSYSSSLERQLLAFQRASEQLSLERGQLEEKYSSLRGDYAIMREDMQ 541
           + M   SEE +  K+Y+ SL  QL A Q +S++++ E   L   Y  ++ DY +   D Q
Sbjct: 509 LKMVEHSEEYEILKNYADSLHYQLTALQNSSKRITQEYESLNTDYVQMKVDYDMQTRDFQ 568

Query: 542 IILGKVNQTMNTIKIMARRARGFAEWARDLRENTSPMASNAEEL 575
           +++ +V+QT+  ++++++RA  FAE A DLR N   M  +A++L
Sbjct: 569 VLVERVDQTIEFLRMVSKRANDFAESAADLRVNFFSMQPHADDL 599

BLAST of Spg031040 vs. ExPASy TrEMBL
Match: A0A5A7T1W2 (Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G00060 PE=4 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 5.8e-107
Identity = 218/506 (43.08%), Postives = 307/506 (60.67%), Query Frame = 0

Query: 62  LKAIWEAVHLKEVQKNVKVKGVEESISADYLIERARKHINEEKGLVLLALCIYGMVLYPR 121
           L    E VH  E+QK +K KG EE++  DYLI+  + +I+E+KGL LLALCIYG V++P+
Sbjct: 89  LSKFLETVHATEIQKYIKAKGGEENVPFDYLIKMTQTYIDEDKGLTLLALCIYGAVIFPK 148

Query: 122 VKGYVDGDVLKLFFSIEQGVDPAIPLLAETVRALNYCKSNKG---------GKLRCCVPL 181
            +GYVD  V+KLFF +E+GV+P IP+LAET R+LNYC+ NKG         GKL CCVPL
Sbjct: 149 AEGYVDRKVIKLFFQMERGVNPIIPILAETFRSLNYCR-NKGEGKLNCCVRGKLNCCVPL 208

Query: 182 LYIWVHSHFKFPEDFGCPKINFSSTWNLTRSTIAEFGTAPWNSSFPRKEAWVSFFSNSKL 241
           LYIW+HSH KFP +F CP+++FSS WNL R+TI+EFG A W+ ++PRKEAW+SFF+    
Sbjct: 209 LYIWIHSHIKFPAEFRCPRLDFSSPWNLMRNTISEFGMAVWDPTYPRKEAWLSFFAKLTS 268

Query: 242 EHVIWKAHWMPLKALIYRCGSFHTVPLVGPWGCVHYAPLLALRQSWFKQFIPATHGLHKL 301
           E+VIWKA WMPLKA+IYRCG FH+VPL+GPWG V+Y PLL LRQ W KQFIP TH L   
Sbjct: 269 ENVIWKAQWMPLKAVIYRCGDFHSVPLLGPWGGVNYTPLLVLRQVWLKQFIPPTHNL--- 328

Query: 302 EFSYSCEDSNEKKRQVLTSWRTVRRINGNSHSEGVTPEYLQWRIKRSK-IPITTRDNVGE 361
                                   +I    H EGVT  Y  W+  R K I   +R+ V  
Sbjct: 329 ------------------------KIKDKGHYEGVTSGYEAWQANRRKNIIDISREVVER 388

Query: 362 SSSRAVDKPNQLATERKELVGRNQTLKLENEKLQQEVKRLMNQENRRLIKENHALRNENA 421
               + ++PNQ   +  EL  +N+ L+ ENEKL++E  + M+            L+NE  
Sbjct: 389 GKETSFEQPNQWIEKSIELEQKNRLLEQENEKLRKETSQWMDHAT--------YLQNELE 448

Query: 422 ALRRATRSQENMIKDLSKGKETLLELVAELNETINKQKTQLIEFEEANTALRRTLDNLRV 481
             +   ++Q+ +  DL    ETL + +  +N+     K +        T L+ T+  L +
Sbjct: 449 KTKSFLKNQDKLETDL----ETLDKEMRRMNKANRSMKNE-------KTTLQATV-GLHL 508

Query: 482 NMQAQSEESKAFKSYSSSLERQLLAFQRASEQLSLERGQLEEKYSSLRGDYAIMREDMQI 541
            M  +SEE K  K+Y+  L  QL A Q +S++++ E   L   Y  ++ DY +   D Q+
Sbjct: 509 KMAERSEEYKILKNYADFLHYQLTALQNSSKRITQEYESLNTDYVQMKVDYDLHTRDFQV 546

Query: 542 ILGKVNQTMNTIKIMARRARGFAEWA 558
           ++ +V+QT+  ++++++RA GFAEWA
Sbjct: 569 LVERVDQTIEFLRMVSKRANGFAEWA 546

BLAST of Spg031040 vs. ExPASy TrEMBL
Match: A0A5A7T1W2 (Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G00060 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 6.4e-13
Identity = 51/133 (38.35%), Postives = 74/133 (55.64%), Query Frame = 0

Query: 671  ISGTGGVTRSGKCYT----PDQLLKFGLGYKPNKADMIRVQKREKEWRLARFESREPEYE 730
            I G GG + +    T    P    +FGLGYKP+  D IR+Q+++K+ RL + E RE +  
Sbjct: 1372 IMGGGGYSLNKNLETLLKIPSNDGRFGLGYKPSIYDKIRLQEKKKKKRLVKLEMREFDPS 1431

Query: 731  GKVIPHLYHSFESAGIIRPGD--------------FAVAVVNKEEEL-GPWIYPCPENFE 785
             K+IP LY +F+SAGI    D               +VA V +E    G  +Y CP +FE
Sbjct: 1432 IKLIPELYDTFKSAGISYSSDNSDLKDDLLTKMESLSVAAVAQEASFEGNTVYACPPDFE 1491

HSP 2 Score: 378.3 bits (970), Expect = 8.1e-101
Identity = 209/518 (40.35%), Postives = 291/518 (56.18%), Query Frame = 0

Query: 12  SSSSEYDELSTVLLWAEQTQLKHGDNLPYKSPVSSSFSSQVQIIANELGELKAIWEAV-- 71
           S  S++DE S VL WAE+ Q K GD +   S +S     Q+    N+L  LK IWEA+  
Sbjct: 9   SFESKFDESSDVLKWAEEMQQKFGDGINNSSQISVLSRCQLSSTKNDLANLKMIWEALTP 68

Query: 72  -----------HL----------------------------------------------- 131
                      H+                                               
Sbjct: 69  QRRFMFSKKYGHIAELMYKPVNYFALRAIINFWDPAYGCFTFGSCDLLPTIEEYQVMLSM 128

Query: 132 --------------------KEVQKNVKVKGVEESISADYLIERARKHINEEKGLVLLAL 191
                                E+QK +KVK  EE++  DYLI+  + +I+E+KGL LLAL
Sbjct: 129 PEKEREIVYFFNPKQTTKTTTEIQKYIKVKDGEENVPFDYLIKMTQTYIDEDKGLTLLAL 188

Query: 192 CIYGMVLYPRVKGYVDGDVLKLFFSIEQGVDPAIPLLAETVRALNYCKSNKGGKLRCCVP 251
           CIYG V++P+ +GYVDG V+KLFF +E+GV+P IP+LA+T R+LNY ++   GK  CCVP
Sbjct: 189 CIYGAVIFPKAEGYVDGKVIKLFFEMERGVNPIIPILAKTFRSLNYYRNKGEGKFNCCVP 248

Query: 252 LLYIWVHSHFKFPEDFGCPKINFSSTWNLTRSTIAEFGTAPWNSSFPRKEAWVSFFSNSK 311
           LLYIW+HSH KFP +F CP+++FSS WNL R+TI+EFG A W+ ++PRKEAW+SFF+   
Sbjct: 249 LLYIWIHSHIKFPAEFRCPRLDFSSPWNLMRNTISEFGMAVWDPTYPRKEAWLSFFAKLT 308

Query: 312 LEHVIWKAHWMPLKALIYRCGSFHTVPLVGPWGCVHYAPLLALRQSWFKQFIPATHGLHK 371
            E+VIWK  WMPLKA+IYR G+FH+VPL+GPWG V+Y PLL LRQ W KQFIP TH L +
Sbjct: 309 SENVIWKTQWMPLKAVIYRRGNFHSVPLLGPWGGVNYTPLLVLRQVWLKQFIPLTHNLQE 368

Query: 372 LEFSYSCEDSNEKKRQVLTSWRTVRRINGNSHSEGVTPEYLQWRI-KRSKIPITTRDNVG 431
            +FSY+ ED   KKRQ + +W+++R+I    H EGV   Y  W+  KR  I   +R+ V 
Sbjct: 369 SDFSYNPEDCQGKKRQAVCTWKSIRKIKDKGHYEGVISGYEAWKANKRKNIIDISREVVE 428

Query: 432 ESSSRAVDKPNQLATERKELVGRNQTLKLENEKLQQEVKRLMNQENRRLIKENHALRNEN 444
           +    +  +PNQ   +  EL  +N+ L+ ENEKL++E  + M          +HA   +N
Sbjct: 429 KGKETSFTQPNQWIEKSIELEEKNRLLEQENEKLRKETSQWM----------DHATYLQN 488

BLAST of Spg031040 vs. ExPASy TrEMBL
Match: A0A5A7UWQ6 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold22G002420 PE=4 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 2.6e-99
Identity = 202/507 (39.84%), Postives = 280/507 (55.23%), Query Frame = 0

Query: 12  SSSSEYDELSTVLLWAEQTQLKHGDNLPYKSPVSSSFSSQVQIIANELGELKAIWEA--- 71
           S  S++DE S VL WAE+ Q K GD++   S +S     Q+    N+L  LK IWEA   
Sbjct: 9   SFESKFDEPSDVLKWAEEMQQKFGDDINNSSQISVLSRCQLSFTQNDLANLKMIWEALTP 68

Query: 72  ------------------------------------------------------------ 131
                                                                       
Sbjct: 69  QRRFMFSKKYGHIVELMYIPVNYFALRAIINFWDPAYGCFTFGSCDLLPTIEEYQAMLSM 128

Query: 132 ---------------------------VHLKEVQKNVKVKGVEESISADYLIERARKHIN 191
                                      VH  E+QK +K+KG EE++  DYLI+  + +++
Sbjct: 129 PEKEREIVYFFNPKQTTKRTLSKFLETVHAAEIQKYIKIKGGEENVPFDYLIKMTQTYVD 188

Query: 192 EEKGLVLLALCIYGMVLYPRVKGYVDGDVLKLFFSIEQGVDPAIPLLAETVRALNYCKSN 251
           E+KGL LLALCIYG V++P+ +GYVDG V+KLFF +E+GV+P IP+LAET R+LNYC++ 
Sbjct: 189 EDKGLTLLALCIYGAVIFPKAEGYVDGKVIKLFFEMERGVNPIIPILAETFRSLNYCRNK 248

Query: 252 KGGKLRCCVPLLYIWVHSHFKFPEDFGCPKINFSSTWNLTRSTIAEFGTAPWNSSFPRKE 311
             GKL CC+PLLYIW+HSH KFP +F CP+++FSS WNL R+TI+EFG A W+ ++PRKE
Sbjct: 249 GEGKLNCCLPLLYIWIHSHIKFPAEFRCPRLDFSSPWNLIRNTISEFGMAVWDPTYPRKE 308

Query: 312 AWVSFFSNSKLEHVIWKAHWMPLKALIYRCGSFHTVPLVGPWGCVHYAPLLALRQSWFKQ 371
           AW+SFF+    ++V+WKA WMPLKA+IYRC  FH++PL+GP G V+Y PLL LRQ W KQ
Sbjct: 309 AWLSFFAKLTSKNVVWKAQWMPLKAVIYRCRDFHSMPLLGPRGGVNYTPLLVLRQVWLKQ 368

Query: 372 FIPATHGLHKLEFSYSCEDSNEKKRQVLTSWRTVRRINGNSHSEGVTPEYLQWRIKRSK- 427
           FIP TH L + +FSY  ED   KKRQ + +W+++R+I    H EGV   Y  W+  R K 
Sbjct: 369 FIPPTHNLQESDFSYDPEDCQGKKRQAVCAWKSIRKIKDKGHYEGVISGYEAWQANRRKN 428