Spg030958 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg030958
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Threonyl-tRNA synthetase
Location	scaffold11: 27284076 .. 27286441 (-)
RNA-Seq Expression	Spg030958
Synteny	Spg030958

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTCAAGACGAGGTGGTTGTGAAGTGCCTGAAGGCTTACGACTATGCTGAACAAAAGGAGGTTTGTGCCTCTAACATTTAGAGGTGTACGCCTCCATGCTTACGCCTTTAAGGCGTTAAAGGCACAGGCCTCATTGCATAATGAAAATTCTTTAGAATTTCTAATAGTAGGCTGGTAGTAGGTTTATGTCTAATGGGTGAGAGTACCCTAATGACATTAATTGTCTTGAAGACTTAAACAATGTGGCATATTATCTAAGTGAAGGTCTCACTATCCTATTCCTAAAATGTAAGCGGTGGCACACTAGAAACAGCAATTATTTGTCACCATTTGGAGAAGAATAGTGTCTGCCACCACACACAGCAAAGCTAATATGTTAGTTCTGTCTGCTTTTATTCTTCTTTTGGTTTCTTGTTTACTATTTCTCCTTTTCACATTTCTTTGATTATTATTTATTGCCACCTCAACCATTTTTTTTAGAAGAAAAAAAGAGTGATAAGGTCTATGTAGGGATGTTTATGAGGTGGAGCAGGGATGGGGATGTGAGTAAGGGAGACTCGAAGAGGTATGAGAGAGTGAGGGTAGCAATGGAGGTGGAGGGAGATGAAATCTACTATCAATAGAGAGGGTGGTGGGTCTGCCACCACCAATGGAACTGAAAGGAAGATTGAAATGGAAAGGGTGGTAACTCTAGGGTTTACATATATAGGTTTAGTTTGATATTTTATTTTATTATTTTTCAATTTCTTTTATCAGTGAGCATTAGTGAATTGAAAATTTTTCATATTCTAAATTATAGAACTGAGATATTGAAACAGTCCTATACAACAAGTAAAGACCTAACAAGACAAAAAAAGAGCTCCAATCCAAAACAATAAGAACAAGAAGTTAATTACAAAAGTCTCCAGAAACAAACACCTAAAAAGAGATGTTAAATTTGACAAGTGCCCTTCCCAAGACCTTTCAAATCCTCTAAAAATTCTCCCATTCTTCTTCAGTAAGATATTCCGTAAAACGGGATTAGAAAAACTAGCATACTAGAGAATTCAAGGCTTATCTTGAAAGGGAAAAATGCTAAAGTCTCCTCCATCATAAGACAACAACTATTGTTATGAGCTAAGCACACATCAAACATATCCACAAAAAGGTTCCACATCGGGCTAGCAACCCTAAATCTCCAAAGGATGTGATCCAAATACTCAAATGCCTTCTACAAAGAATGTATCACTATGGTGTGGTCTCATCAAGAAGGGAGAATGCCTTTGAATACAGTCCATAGTGTTGACTGTCCCATGTAAGCCCTTCTAGGTGGTGGTTGACTTGCTGAAATAAGTTAACAGTTTTGTAATTTCAAAGGGGATAACTATTGCAGTTGGGATAAACTTCTTGTTGCACTGTCCAAGTTGACTTATATAGGGGTGATAGAGTTTTAACACCACATTTAATGTCCCTTTAATTTTTATGCTAGTAGTTCGGTTCCTTATAGATTAATTGGACTTTCATGACAGGGATTCTATTACGATGCTTTTTATGATGATTTGGGCTTAAATGATGATCACTTCAAGCAAATTGAGTCTGGTGCACTGAAGGCTGTATCGGTATTGTTCTTTCTCTTGACAAAGAGTTTACACCATTTATTTTACATAATTAAAATCTGGTTTTATATATTTTTCCATGTATTTCACATGGAAGAAAGATGTTAATAGTTATATAAAGAATGTCCGTCACTATTTCTTTCTTTCTTCCGTTATTATTTTTTCTTGAGAGGGAGAGGACTGGTGTTGCTTGCAATCTTGTTATCTTTCTCATGCAAAAGGCCCAACAACTAGGTTTTGGGATCAATTTTGTATGCTGTAATGCTATGCAGGAGAATCCTCATCCTAACCCTAGTTAACTTTATCTTCATTTGTTATAAAGAATGTAAAAGATTCCGTGCAAGTAAACTAACCTTCTGGTTGTTCCAATTTTTGGAACTAGTAGCAAATCAATAATTTGACAATTGATATCAAAATGTATGATCAGCTAGAAACTCGTAGTGCATATCAGTTGCTTCCTCAGCTTTAGCAAGTGCTTGCTTATCATCTACCTATTGACTGTATTAGCGACTTTCTTGGCTAAAAAATGAAGTGCTGGAAGATTATTCCCAATAAAGTATTCTATACTCTGTATTCATGCATGAAATGTCATATCATTACAGAGGATACTTTTTCTCAGAGGAATAATTCGATTTATTTCTTTTAAATTTTCAGGAGAGACAACCTTTTGAGCGCATTGAAGTTTCAAGGCAACAAGCTCTTGAAATGTTTTCTGACAATAAATTCAAGGCAATTGAATATCACTCAAAAGATTTGCAGTTCATTTGA

mRNA sequence

ATGCCTCAAGACGAGGTGGTTGTGAAGTGCCTGAAGGCTTACGACTATGCTGAACAAAAGGAGGGATTCTATTACGATGCTTTTTATGATGATTTGGGCTTAAATGATGATCACTTCAAGCAAATTGAGTCTGGTGCACTGAAGGCTGTATCGGAGAGACAACCTTTTGAGCGCATTGAAGTTTCAAGGCAACAAGCTCTTGAAATGTTTTCTGACAATAAATTCAAGGCAATTGAATATCACTCAAAAGATTTGCAGTTCATTTGA

Coding sequence (CDS)

ATGCCTCAAGACGAGGTGGTTGTGAAGTGCCTGAAGGCTTACGACTATGCTGAACAAAAGGAGGGATTCTATTACGATGCTTTTTATGATGATTTGGGCTTAAATGATGATCACTTCAAGCAAATTGAGTCTGGTGCACTGAAGGCTGTATCGGAGAGACAACCTTTTGAGCGCATTGAAGTTTCAAGGCAACAAGCTCTTGAAATGTTTTCTGACAATAAATTCAAGGCAATTGAATATCACTCAAAAGATTTGCAGTTCATTTGA

Protein sequence

MPQDEVVVKCLKAYDYAEQKEGFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFKAIEYHSKDLQFI

Homology

[Close]

BLAST of Spg030958 vs. NCBI nr
Match: KAG7032420.1 (Threonine--tRNA ligase, mitochondrial 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 118.2 bits (295), Expect = 3.5e-23
Identity = 57/67 (85.07%), Postives = 61/67 (91.04%), Query Frame = 0


Query: 22  GFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFKAIEYH 81
           GFYYD FYDDLGLNDDHFKQIESGALKAVSE+QPFERIEV+RQQALEMFSDNKFKAIEY 
Sbjct: 36  GFYYDGFYDDLGLNDDHFKQIESGALKAVSEKQPFERIEVTRQQALEMFSDNKFKAIEYC 95

Query: 82  SKDLQFI 89
             +  F+
Sbjct: 96  CNNFHFL 102

[Close]

BLAST of Spg030958 vs. NCBI nr
Match: KAG7029174.1 (Threonine--tRNA ligase, mitochondrial 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 115.9 bits (289), Expect = 1.7e-22
Identity = 58/60 (96.67%), Postives = 58/60 (96.67%), Query Frame = 0


Query: 21  EGFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFKAIEY 80
           EGFYYDAFYDDL LNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFKAI Y
Sbjct: 192 EGFYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFKAIGY 251

[Close]

BLAST of Spg030958 vs. NCBI nr
Match: XP_023527929.1 (threonine--tRNA ligase, mitochondrial 1-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 113.2 bits (282), Expect = 1.1e-21
Identity = 55/56 (98.21%), Postives = 56/56 (100.00%), Query Frame = 0


Query: 21  EGFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 77
           EGFYYDAFYDDLGLNDDHFK+IESGALKAVSERQPFERIEVSRQQALEMFSDNKFK
Sbjct: 142 EGFYYDAFYDDLGLNDDHFKKIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 197

[Close]

BLAST of Spg030958 vs. NCBI nr
Match: XP_038891995.1 (threonine--tRNA ligase, mitochondrial 1 [Benincasa hispida])

HSP 1 Score: 112.5 bits (280), Expect = 1.9e-21
Identity = 54/56 (96.43%), Postives = 56/56 (100.00%), Query Frame = 0


Query: 21  EGFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 77
           EGFYYDAFYDDLGLNDDHFKQIESGALKAVSE+QPFERIEV+RQQALEMFSDNKFK
Sbjct: 190 EGFYYDAFYDDLGLNDDHFKQIESGALKAVSEKQPFERIEVTRQQALEMFSDNKFK 245

[Close]

BLAST of Spg030958 vs. NCBI nr
Match: XP_004142299.1 (threonine--tRNA ligase, mitochondrial 1 [Cucumis sativus] >KAE8648246.1 hypothetical protein Csa_018476 [Cucumis sativus])

HSP 1 Score: 112.5 bits (280), Expect = 1.9e-21
Identity = 54/56 (96.43%), Postives = 56/56 (100.00%), Query Frame = 0


Query: 21  EGFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 77
           EGFYYDAFYDDLGLNDDHFKQIESGALKAVSE+QPFERIEV+RQQALEMFSDNKFK
Sbjct: 190 EGFYYDAFYDDLGLNDDHFKQIESGALKAVSEKQPFERIEVTRQQALEMFSDNKFK 245

[Close]

BLAST of Spg030958 vs. ExPASy Swiss-Prot
Match: O04630 (Threonine--tRNA ligase, mitochondrial 1 OS=Arabidopsis thaliana OX=3702 GN=THRRS PE=1 SV=3)

HSP 1 Score: 84.7 bits (208), Expect = 5.6e-16
Identity = 39/56 (69.64%), Postives = 46/56 (82.14%), Query Frame = 0


Query: 21  EGFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 77
           EGFYYD FY +LGL+D+HF  IE+GA KA  E QPFERIEV++ QALEMFS+N FK
Sbjct: 175 EGFYYDGFYGELGLSDNHFPSIEAGAAKAAKEAQPFERIEVTKDQALEMFSENNFK 230

[Close]

BLAST of Spg030958 vs. ExPASy Swiss-Prot
Match: Q0V9S0 (Threonine--tRNA ligase 2, cytoplasmic OS=Xenopus tropicalis OX=8364 GN=tars3 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 9.6e-08
Identity = 26/57 (45.61%), Postives = 37/57 (64.91%), Query Frame = 0


Query: 20  KEGFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 77
           + GFYYD + D  G++ + F  +E+     + E+QPFER+EVS+   LEMF  NKFK
Sbjct: 271 ENGFYYDMYLDGRGVSSNEFPSLENMCKAIIKEKQPFERLEVSKDLLLEMFKYNKFK 327

[Close]

BLAST of Spg030958 vs. ExPASy Swiss-Prot
Match: Q3ZBV8 (Threonine--tRNA ligase 1, cytoplasmic OS=Bos taurus OX=9913 GN=TARS1 PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 6.2e-07
Identity = 24/57 (42.11%), Postives = 38/57 (66.67%), Query Frame = 0


Query: 20  KEGFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 77
           + GFYYD + ++ G++ + F  +E+   K + E+Q FER+EV ++  LEMF  NKFK
Sbjct: 180 ENGFYYDMYLEEGGVSSNDFSSLETLCKKIIKEKQAFERLEVKKETLLEMFKYNKFK 236

[Close]

BLAST of Spg030958 vs. ExPASy Swiss-Prot
Match: Q9D0R2 (Threonine--tRNA ligase 1, cytoplasmic OS=Mus musculus OX=10090 GN=Tars1 PE=1 SV=2)

HSP 1 Score: 54.7 bits (130), Expect = 6.2e-07
Identity = 24/57 (42.11%), Postives = 38/57 (66.67%), Query Frame = 0


Query: 20  KEGFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 77
           + GFYYD + ++ G++ + F  +E+   K + E+Q FER+EV ++  LEMF  NKFK
Sbjct: 179 ENGFYYDMYLEEGGVSSNDFSSLETLCKKIIKEKQTFERLEVKKETLLEMFKYNKFK 235

[Close]

BLAST of Spg030958 vs. ExPASy Swiss-Prot
Match: Q5XHY5 (Threonine--tRNA ligase 1, cytoplasmic OS=Rattus norvegicus OX=10116 GN=Tars1 PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 6.2e-07
Identity = 24/57 (42.11%), Postives = 38/57 (66.67%), Query Frame = 0


Query: 20  KEGFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 77
           + GFYYD + ++ G++ + F  +E+   K + E+Q FER+EV ++  LEMF  NKFK
Sbjct: 152 ENGFYYDMYLEEGGVSSNDFSSLETLCKKIIKEKQTFERLEVKKETLLEMFKYNKFK 208

[Close]

BLAST of Spg030958 vs. ExPASy TrEMBL
Match: A0A0A0KJG3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G080790 PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 9.2e-22
Identity = 54/56 (96.43%), Postives = 56/56 (100.00%), Query Frame = 0


Query: 21  EGFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 77
           EGFYYDAFYDDLGLNDDHFKQIESGALKAVSE+QPFERIEV+RQQALEMFSDNKFK
Sbjct: 190 EGFYYDAFYDDLGLNDDHFKQIESGALKAVSEKQPFERIEVTRQQALEMFSDNKFK 245

[Close]

BLAST of Spg030958 vs. ExPASy TrEMBL
Match: A0A6J1DR46 (Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111023566 PE=3 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 9.2e-22
Identity = 54/56 (96.43%), Postives = 56/56 (100.00%), Query Frame = 0


Query: 21  EGFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 77
           EGFYYDAFYDDLGLNDDHFKQIESGALKAVSE+QPFERIEV+RQQALEMFSDNKFK
Sbjct: 200 EGFYYDAFYDDLGLNDDHFKQIESGALKAVSEKQPFERIEVTRQQALEMFSDNKFK 255

[Close]

BLAST of Spg030958 vs. ExPASy TrEMBL
Match: A0A6J1F237 (Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111439084 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.1e-21
Identity = 55/56 (98.21%), Postives = 55/56 (98.21%), Query Frame = 0


Query: 21  EGFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 77
           EGFYYDAFYDDL LNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK
Sbjct: 192 EGFYYDAFYDDLCLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 247

[Close]

BLAST of Spg030958 vs. ExPASy TrEMBL
Match: A0A6J1DG60 (Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111020133 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.7e-21
Identity = 53/56 (94.64%), Postives = 55/56 (98.21%), Query Frame = 0


Query: 21  EGFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 77
           EGFYYD FYDDLGLNDDHFKQIESGALKAVSE+QPFERIEV+RQQALEMFSDNKFK
Sbjct: 179 EGFYYDGFYDDLGLNDDHFKQIESGALKAVSEKQPFERIEVTRQQALEMFSDNKFK 234

[Close]

BLAST of Spg030958 vs. ExPASy TrEMBL
Match: A0A6J1DER8 (Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111020133 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.7e-21
Identity = 53/56 (94.64%), Postives = 55/56 (98.21%), Query Frame = 0


Query: 21  EGFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 77
           EGFYYD FYDDLGLNDDHFKQIESGALKAVSE+QPFERIEV+RQQALEMFSDNKFK
Sbjct: 142 EGFYYDGFYDDLGLNDDHFKQIESGALKAVSEKQPFERIEVTRQQALEMFSDNKFK 197

[Close]

BLAST of Spg030958 vs. TAIR 10
Match: AT5G26830.1 (Threonyl-tRNA synthetase )

HSP 1 Score: 84.7 bits (208), Expect = 4.0e-17
Identity = 39/56 (69.64%), Postives = 46/56 (82.14%), Query Frame = 0


Query: 21  EGFYYDAFYDDLGLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 77
           EGFYYD FY +LGL+D+HF  IE+GA KA  E QPFERIEV++ QALEMFS+N FK
Sbjct: 175 EGFYYDGFYGELGLSDNHFPSIEAGAAKAAKEAQPFERIEVTKDQALEMFSENNFK 230

The following BLAST results are available for this feature:

BLAST of Spg030958 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7032420.1	3.5e-23	85.07	Threonine--tRNA ligase, mitochondrial 1, partial [Cucurbita argyrosperma subsp. ...	[more]
KAG7029174.1	1.7e-22	96.67	Threonine--tRNA ligase, mitochondrial 1 [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_023527929.1	1.1e-21	98.21	threonine--tRNA ligase, mitochondrial 1-like, partial [Cucurbita pepo subsp. pep...	[more]
XP_038891995.1	1.9e-21	96.43	threonine--tRNA ligase, mitochondrial 1 [Benincasa hispida]	[more]
XP_004142299.1	1.9e-21	96.43	threonine--tRNA ligase, mitochondrial 1 [Cucumis sativus] >KAE8648246.1 hypothet...	[more]

BLAST of Spg030958 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04630	5.6e-16	69.64	Threonine--tRNA ligase, mitochondrial 1 OS=Arabidopsis thaliana OX=3702 GN=THRRS...	[more]
Q0V9S0	9.6e-08	45.61	Threonine--tRNA ligase 2, cytoplasmic OS=Xenopus tropicalis OX=8364 GN=tars3 PE=...	[more]
Q3ZBV8	6.2e-07	42.11	Threonine--tRNA ligase 1, cytoplasmic OS=Bos taurus OX=9913 GN=TARS1 PE=2 SV=1	[more]
Q9D0R2	6.2e-07	42.11	Threonine--tRNA ligase 1, cytoplasmic OS=Mus musculus OX=10090 GN=Tars1 PE=1 SV=...	[more]
Q5XHY5	6.2e-07	42.11	Threonine--tRNA ligase 1, cytoplasmic OS=Rattus norvegicus OX=10116 GN=Tars1 PE=...	[more]

BLAST of Spg030958 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KJG3	9.2e-22	96.43	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G080790 PE=4 SV=1	[more]
A0A6J1DR46	9.2e-22	96.43	Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111023566 PE=3 SV=...	[more]
A0A6J1F237	2.1e-21	98.21	Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111439084 PE=3 SV=1	[more]
A0A6J1DG60	2.7e-21	94.64	Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111020133 PE=3 SV=...	[more]
A0A6J1DER8	2.7e-21	94.64	Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111020133 PE=3 SV=...	[more]

BLAST of Spg030958 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G26830.1	4.0e-17	69.64	Threonyl-tRNA synthetase	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.980.10		coord: 5..85 e-value: 3.4E-12 score: 48.6
None	No IPR available	PANTHER	PTHR11451:SF53	THREONINE--TRNA LIGASE, CYTOPLASMIC	coord: 17..77
IPR002320	Threonine-tRNA ligase, class IIa	PANTHER	PTHR11451	THREONINE-TRNA LIGASE	coord: 17..77
IPR018163	Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily	SUPERFAMILY	55186	ThrRS/AlaRS common domain	coord: 20..78

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg030958.1	Spg030958.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006435	threonyl-tRNA aminoacylation
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004829	threonine-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding