Spg030842 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg030842
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionO-methyltransferase
Locationscaffold11: 27377591 .. 27380962 (+)
RNA-Seq ExpressionSpg030842
SyntenySpg030842
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCCTTTCTGAGTTAGTGGAAGTCCTCCACATCCAACCCTCTAAATCTCAATGCCTTGGCCGCCTTATGCGACTCCTCGTTCATTCCGGCTTCTTTTCTCAAATTCATGGCGAGAATGAGCAACAACTCAAGTATTCTCTCACACCCTCCTCTCGATTTCTCCTCCATCACGATACCACATTAGAAACCTTACCCTTCTTGTTTCTCGTTCTCCATAAAGCCATGATGGCCCCATGGGAAACCATGAGCAGTTGGTTGTGTAGTGCAGATGATCAGTGTTCTACTGCCTTTGAAATGGCGAATGGGAAGTCGATATGGGATTATATTGCAGAGGAGCCTGATTTTGGCAATTTGTTCCATCAAACAATGGAGTACGATTCTCAACTAATTAGGAGATTAGTGACGAGGGGGTGCCCAGAGGTGTTTGAAGGGTTGAAGTCCATGGTCGATGTTGGTGGCGGCGCAGGCATTATGGCTAAGGCCATCATGGAAGCATTCCCACACATCACTTGCACTGCGTTCGATCTCCAACAAATGGTTTCCAACCACCAGAAAATCTCAAAAGATCACTTGAACTTCATTGAAGGAGATATCTTTGAAGCAAAAATTCCACCAACAGATGCAATTTTACTCCAGGTATATATGTTTCTCGTCTATAATTTTCTAAGACTAGTAAAAGTTATTTATCGTATTCTTCGGGATTTTGGTAATCAGTCAGTTCTACACAAGTGGAATGATGAAGAAAGCGTACGAATACTGAAGAATTGTAAAGATGCAATTCGTGGTGCTAAAGGTGGGAAAGTGATAATCATAGAGGCAGTGCTGGAAAACCTGATGGAAGATAAGGAGTCGACTGAAACTCAACTTTGTGGTGATGTGTTGATGATGGCCAGTTTCACCGGTCGAAAGAGAAATGAGAGAGAATGGAAGAGTCTCTTCGTAGCAGCTGGCTTTAGTGACTATAAGATCACTTCTTTTCTGGGTTTAAGGTCTCTCATTGAGGTGTACCCTTGATAATTTGAGCACCTTCTAATCCTTTATAACATCTACCTGTATTGCAAATCCAATAAATGTTGTGCCCTGGTTTAGTTAATGTACTGAAGAAGAGAATAAAAATCCATTCATGATGAAAGATTGTGAATTTAAGAACAAAATTATGATTATGTTTACTAAGATTACTTTGAAATCCTAATTATAAAAACCATGTATCATGATGCAGATGATTTGATACTTGACCATGCTTCAAATTTAAACAAAGGACGATCGAATTCAATTCAATGGTAAAAATAGATGCAACCAATCTGGTTTGATTAGCTTCTACCTTAATGGTTTGGGTTGGGTTCCATTCAATCATAATAAAATAGAGGAAACTTAGTCTGAATCTTGAGTGGATTATGGACTCCTGTTCACGAGGGATTGTCCTTTGATTTGTATGGGTGAGAGTGGCTAGTACGCCGACTCAATAAGCCTACCATTTTGGGGACAAGACTGAGTGGGGAGCTGGAAACATAATCACACAAGATGGAATTCACTCTTTCCCGACTTTCGAGGAAGTAGATGAATGTTCCCTTAAGTGGTGTCTCCGGGTCTTGAACAAACGACCTCTCACTGGCCCTAGAGGGTTTTCTGTTTGATGGATGGACCACAAACAGGTTGTTTATTAGAGGAGCACTGATACTTAAGGAGGTAGAGGTAACTAAGGGGTGAAACGGTAATTTGACCCAACTGGAGTTACGAACACTCGTGAAGGACTAACTTGCTGTTATTGGTCGATATCCGTGGACACAGAAATATTCTGCAGTGAGAAAAGTGCAGTTGTGGGTCTTTAGTGGAGTGACCTACAGTAAACGAATGTTGACTAACTTAGTTAATGAGTTTAGCCAATTAGTCTCGTATCGTTGGAGCTTCTGATCTGTGGTCCATTAGGTCCCACTGGTAGCTCGTAAAGAGACTTGAGACAATTAAGTATAAATTGTAAATTGGAAGTGTTCAATTTATTTAGGGGAATTATATAATGTATATTGATACATTATAATATAAAGTTTATGAAACTTTATAATATAATTTTTGAATTAATTTCAAAAATTAATTCTTGAGAGAAAATATTTGAAAGAGTTCAAATAATTAATATAAATCAGATTCATATTAATATAAATAATTTAATAAAACTCCTAAGATTAAATATGTGATATTTAATTGGGTAATTAATTAATTATGGAATTAATTAATTATTTATTAAAATTGGATATGAATAAGATAATAGATTTGATCTTATTCATGACTTTTTCCTATTAGGAAAAGGTCTTGGCCTTTACCTATAAATAGGACTCCTGGGGTGGGGTGAAAGGCATTCCATTCATTCTACTCCTCTCAGAGATCTCTCCTATAAGACTCCCACCAGTCTCCTGCCTCCAGTAGTCTCAGAGATATACCGATGTAACCAATTGGTGGTGTTCGCACAAGAAGGTTGCTGTGTTTTCGATCTCACTAGCAAGAAAAGGCGAATCGATCAAGGCTTCCTACGAAAGTGAGTTTCCTTTGAAAACCTTGATCACTAGGATCATCTAGATTAGATCTAGACAATTATTAAGCATGTTTATAGCCGCCGATCGCCGGACTTAGGAAGAAGAAGAGGAGGAGGGAAAAGATGGAGAAGAAGGAAGATGGAGAAGAAGATGAGAGAGAAAGAGAGAGAATAAAAAAAATAATTAAATATTAATATTTTATTTTTTTAATATTTTTTATTTAAAAAAATAAAAAAAAATAATTTTTTATATTAAAATAATAGTGCCACGTTAGCAACCGTTAAAAGTTAACAGAAAATTTGACGGCAGGGACCAAAGTGCTCCTGATCTCAAACCTCAGGGGTATTTTTGTTGAGTTTGAAAACTCAGGGACCAAAGTGTCACTTAACCCAAACCTCAGGGACCAAAAGTGTATTTTTCCCTTTTGTAAATCAATTATCCTCTGTAAGTTCGGGTCTAGAATAGGGTATTTTATGATGAAGATCGATGAAAATCACGTCCAAAATCGAGGCCGAATGCGAAGAACAATTGCTGAAATGCCCAAATTCTGCCTCCTGACGCAATAGCGTCGCGACCCTGTTCATCAGGCGTTCTGACGCTGTCGCTATTTCGCGAAGCGGAGATCTGCGATCGCATAAGCGTCGCGACGCTATCTTCCCAGCGTCTCGACGCTGACGCGATTTCATGCGGCGTGCGACTAGGGTTTCATGAGGAGCGGCTGATGGATATTTGTGGTCCGGTTCACTGGTCTGGAGTCGGTTTGGCCTGGTCCAGCCCGGTTCAAGCTTGTTTTGGTGATTTGGAGTCCAGTTCGAGGCGGTTCGGGTCAGTTCGTGCGGTCTAG

mRNA sequence

ATGAGCCTTTCTGAGTTAGTGGAAGTCCTCCACATCCAACCCTCTAAATCTCAATGCCTTGGCCGCCTTATGCGACTCCTCGTTCATTCCGGCTTCTTTTCTCAAATTCATGGCGAGAATGAGCAACAACTCAAGTATTCTCTCACACCCTCCTCTCGATTTCTCCTCCATCACGATACCACATTAGAAACCTTACCCTTCTTGTTTCTCGTTCTCCATAAAGCCATGATGGCCCCATGGGAAACCATGAGCAGTTGGTTGTGTAGTGCAGATGATCAGTGTTCTACTGCCTTTGAAATGGCGAATGGGAAGTCGATATGGGATTATATTGCAGAGGAGCCTGATTTTGGCAATTTGTTCCATCAAACAATGGAGTACGATTCTCAACTAATTAGGAGATTAGTGACGAGGGGGTGCCCAGAGGTGTTTGAAGGGTTGAAGTCCATGGTCGATGTTGGTGGCGGCGCAGGCATTATGGCTAAGGCCATCATGGAAGCATTCCCACACATCACTTGCACTGCGTTCGATCTCCAACAAATGGTTTCCAACCACCAGAAAATCTCAAAAGATCACTTGAACTTCATTGAAGGAGATATCTTTGAAGCAAAAATTCCACCAACAGATGCAATTTTACTCCAGGTATATATGTTTCTCGTCTATAATTTTCTAAGACTAGTAAAAGTTATTTATCGTATTCTTCGGGATTTTGGTAATCAGTCAGTTCTACACAAGTGGAATGATGAAGAAAGCGTACGAATACTGAAGAATTGTAAAGATGCAATTCGTGGTGCTAAAGGTGGGAAAGTGATAATCATAGAGGCAGTGCTGGAAAACCTGATGGAAGATAAGGAGTCGACTGAAACTCAACTTTGTGGTGATGTGTTGATGATGGCCAGTTTCACCGGTCGAAAGAGAAATGAGAGAGAATGGAAGAGTCTCTTCGTAGCAGCTGGCTTTAGTGACTATAAGATCACTTCTTTTCTGGGACTCCTGGGGTGGGGTGAAAGGCATTCCATTCATTCTACTCCTCTCAGAGATCTCTCCTATAAGACTCCCACCAGTCTCCTGCCTCCAGTAGTCTCAGAGATATACCGATGCGTTCTGACGCTGTCGCTATTTCGCGAAGCGGAGATCTGCGATCGCATAAGCGTCGCGACGCTATCTTCCCAGCGTCTCGACGCTGACGCGATTTCATGCGGCGTGCGACTAGGGTTTCATGAGGAGCGGCTGATGGATATTTGTGGTCCGGTTCACTGGTCTGGAGTCGGTTTGGCCTGGTCCAGCCCGGTTCAAGCTTGTTTTGGTGATTTGGAGTCCAGTTCGAGGCGGTTCGGGTCAGTTCGTGCGGTCTAG

Coding sequence (CDS)

ATGAGCCTTTCTGAGTTAGTGGAAGTCCTCCACATCCAACCCTCTAAATCTCAATGCCTTGGCCGCCTTATGCGACTCCTCGTTCATTCCGGCTTCTTTTCTCAAATTCATGGCGAGAATGAGCAACAACTCAAGTATTCTCTCACACCCTCCTCTCGATTTCTCCTCCATCACGATACCACATTAGAAACCTTACCCTTCTTGTTTCTCGTTCTCCATAAAGCCATGATGGCCCCATGGGAAACCATGAGCAGTTGGTTGTGTAGTGCAGATGATCAGTGTTCTACTGCCTTTGAAATGGCGAATGGGAAGTCGATATGGGATTATATTGCAGAGGAGCCTGATTTTGGCAATTTGTTCCATCAAACAATGGAGTACGATTCTCAACTAATTAGGAGATTAGTGACGAGGGGGTGCCCAGAGGTGTTTGAAGGGTTGAAGTCCATGGTCGATGTTGGTGGCGGCGCAGGCATTATGGCTAAGGCCATCATGGAAGCATTCCCACACATCACTTGCACTGCGTTCGATCTCCAACAAATGGTTTCCAACCACCAGAAAATCTCAAAAGATCACTTGAACTTCATTGAAGGAGATATCTTTGAAGCAAAAATTCCACCAACAGATGCAATTTTACTCCAGGTATATATGTTTCTCGTCTATAATTTTCTAAGACTAGTAAAAGTTATTTATCGTATTCTTCGGGATTTTGGTAATCAGTCAGTTCTACACAAGTGGAATGATGAAGAAAGCGTACGAATACTGAAGAATTGTAAAGATGCAATTCGTGGTGCTAAAGGTGGGAAAGTGATAATCATAGAGGCAGTGCTGGAAAACCTGATGGAAGATAAGGAGTCGACTGAAACTCAACTTTGTGGTGATGTGTTGATGATGGCCAGTTTCACCGGTCGAAAGAGAAATGAGAGAGAATGGAAGAGTCTCTTCGTAGCAGCTGGCTTTAGTGACTATAAGATCACTTCTTTTCTGGGACTCCTGGGGTGGGGTGAAAGGCATTCCATTCATTCTACTCCTCTCAGAGATCTCTCCTATAAGACTCCCACCAGTCTCCTGCCTCCAGTAGTCTCAGAGATATACCGATGCGTTCTGACGCTGTCGCTATTTCGCGAAGCGGAGATCTGCGATCGCATAAGCGTCGCGACGCTATCTTCCCAGCGTCTCGACGCTGACGCGATTTCATGCGGCGTGCGACTAGGGTTTCATGAGGAGCGGCTGATGGATATTTGTGGTCCGGTTCACTGGTCTGGAGTCGGTTTGGCCTGGTCCAGCCCGGTTCAAGCTTGTTTTGGTGATTTGGAGTCCAGTTCGAGGCGGTTCGGGTCAGTTCGTGCGGTCTAG

Protein sequence

MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFFSQIHGENEQQLKYSLTPSSRFLLHHDTTLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIAEEPDFGNLFHQTMEYDSQLIRRLVTRGCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHITCTAFDLQQMVSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIYRILRDFGNQSVLHKWNDEESVRILKNCKDAIRGAKGGKVIIIEAVLENLMEDKESTETQLCGDVLMMASFTGRKRNEREWKSLFVAAGFSDYKITSFLGLLGWGERHSIHSTPLRDLSYKTPTSLLPPVVSEIYRCVLTLSLFREAEICDRISVATLSSQRLDADAISCGVRLGFHEERLMDICGPVHWSGVGLAWSSPVQACFGDLESSSRRFGSVRAV
Homology
BLAST of Spg030842 vs. NCBI nr
Match: XP_022152414.1 (probable O-methyltransferase 3 [Momordica charantia])

HSP 1 Score: 445.7 bits (1145), Expect = 4.9e-121
Identity = 228/332 (68.67%), Postives = 264/332 (79.52%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFFSQIHGEN-EQQLKYSLTPSSRFLLHHD 60
           MSLS+LVE LHIQPSK+QCLGRLMRLLVHSGFF++ H EN EQ+ KY++TPSSR LL H+
Sbjct: 46  MSLSDLVEALHIQPSKAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHN 105

Query: 61  TTLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIAEEPDFGNL 120
            TLETLPFLFL+LH AMMA WETMS WLCS D+  S+AFEMANGKSIW+YIAEEP+FGNL
Sbjct: 106 DTLETLPFLFLILHHAMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNL 165

Query: 121 FHQTMEYDSQLIRRLVTRGCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHITCTAFDLQQ 180
           FH+T+ YDSQLI R+VT  C EVFEGLKS+VDVGGG GIMAKAI+EAFPHIT   FDL Q
Sbjct: 166 FHRTIAYDSQLIGRIVTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQ 225

Query: 181 MVSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIYRILRDFGNQ 240
           +V+NHQ+ +K +L+FI GD+FEAKIPPT+A+LL                          +
Sbjct: 226 VVANHQQTTK-NLSFIGGDMFEAKIPPTNAVLL--------------------------K 285

Query: 241 SVLHKWNDEESVRILKNCKDAI-RGAKGGKVIIIEAVLENLMEDKESTETQLCGDVLMMA 300
           SVL+ WNDEES++ILKNCKDAI   A+GGKVIIIE  LE  +EDKES ETQLCGDVLMM 
Sbjct: 286 SVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQVEDKESIETQLCGDVLMMT 345

Query: 301 SFTGRKRNEREWKSLFVAAGFSDYKITSFLGL 331
           +F  RKRNEREWK+LF+AAGFSDYKI   LGL
Sbjct: 346 NFAIRKRNEREWKNLFLAAGFSDYKIIPMLGL 350

BLAST of Spg030842 vs. NCBI nr
Match: XP_022158734.1 (probable O-methyltransferase 3 [Momordica charantia])

HSP 1 Score: 416.4 bits (1069), Expect = 3.2e-112
Identity = 209/331 (63.14%), Postives = 255/331 (77.04%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFFSQIHGENEQQLKYSLTPSSRFLLHHDT 60
           MSLS LV+ LHIQPSK+QCL RLMRLLVH+GFF Q HG+NEQ+ +Y+LTPSSR LL  +T
Sbjct: 46  MSLSNLVDALHIQPSKAQCLNRLMRLLVHAGFFIQTHGDNEQE-EYALTPSSRLLLCRNT 105

Query: 61  TLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIAEEPDFGNLF 120
             E +PF+FL+L++AMMAP ETMSSWLCS D+  ++AFEMANGKSIWDY+++E DFG LF
Sbjct: 106 AFENIPFVFLILNRAMMAPLETMSSWLCSVDEHSNSAFEMANGKSIWDYVSQEHDFGKLF 165

Query: 121 HQTMEYDSQLIRRLVTRGCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHITCTAFDLQQM 180
           HQTM +DSQLI ++VTR C EVFEGLKS+VDVGG  G MAKAI EAFPHITCT FDL Q+
Sbjct: 166 HQTMVHDSQLIGKVVTRECREVFEGLKSLVDVGGSTGFMAKAIAEAFPHITCTVFDLPQV 225

Query: 181 VSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIYRILRDFGNQS 240
            +N Q+ +K +L+FI GD+FEAKIPP +A+LL                          + 
Sbjct: 226 AANSQQTTK-NLSFIGGDMFEAKIPPANAVLL--------------------------KW 285

Query: 241 VLHKWNDEESVRILKNCKDAIRG-AKGGKVIIIEAVLENLMEDKESTETQLCGDVLMMAS 300
           +LH WNDEES+RILKNCKDAI   A+ GKVI+I+ V+EN +EDKES E QLC D+L+M S
Sbjct: 286 ILHDWNDEESIRILKNCKDAIPSRAEXGKVIVIDMVVENQIEDKESVEAQLCCDLLVMTS 345

Query: 301 FTGRKRNEREWKSLFVAAGFSDYKITSFLGL 331
           + GR+RNEREWK+LF+ AGFSDYKI S LGL
Sbjct: 346 YNGRERNEREWKNLFLTAGFSDYKIISMLGL 348

BLAST of Spg030842 vs. NCBI nr
Match: XP_022152376.1 (trans-resveratrol di-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 383.3 bits (983), Expect = 3.0e-102
Identity = 198/331 (59.82%), Postives = 238/331 (71.90%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFFSQIHGENEQQLKYSLTPSSRFLLHHDT 60
           MSLS LV  LHIQP K+ CLGRLM LLVHSGFF Q H ENEQ++KY+LT +SR LL H+T
Sbjct: 46  MSLSNLVGALHIQPFKAHCLGRLMSLLVHSGFFVQTHDENEQEVKYTLTSTSRLLLRHNT 105

Query: 61  TLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIAEEPDFGNLF 120
             E +  L L+ +KAMMA +ETMSSW  S D++ S AFE  N KS+WDY+A+EPDF N+ 
Sbjct: 106 PFEHISLLLLIFNKAMMASYETMSSWFRSTDERHSCAFETTNDKSVWDYVAQEPDFSNIL 165

Query: 121 HQTMEYDSQLIRRLVTRGCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHITCTAFDLQQM 180
           ++ M YDSQLI R+VTR C  VF+GLKS+VDVGG  G MAKAI EAFPHITC  FDL  +
Sbjct: 166 NKAMMYDSQLIGRVVTRECRRVFKGLKSLVDVGGNRGFMAKAIAEAFPHITCIVFDLPHV 225

Query: 181 VSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIYRILRDFGNQS 240
           V N Q+ + ++LNFI G++FEAKIPP +A+LL                          + 
Sbjct: 226 VDNLQQ-TTENLNFIAGNMFEAKIPPANAVLL--------------------------KW 285

Query: 241 VLHKWNDEESVRILKNCKDAIRG-AKGGKVIIIEAVLENLMEDKESTETQLCGDVLMMAS 300
           VLH WNDE+S++ILKNCKDAI   A+GGKVIIIE V EN +EDKES ETQLC DV  M+ 
Sbjct: 286 VLHDWNDEKSIKILKNCKDAIPSRAEGGKVIIIEMVTENQIEDKESIETQLCEDVFTMSV 345

Query: 301 FTGRKRNEREWKSLFVAAGFSDYKITSFLGL 331
            TGR+RNE EWK+LF+AAGFSDYKI S LGL
Sbjct: 346 VTGRERNESEWKNLFLAAGFSDYKIISMLGL 349

BLAST of Spg030842 vs. NCBI nr
Match: XP_023520750.1 (probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo] >XP_023528579.1 probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 366.3 bits (939), Expect = 3.8e-97
Identity = 201/340 (59.12%), Postives = 236/340 (69.41%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFFSQIHGENEQQ---LKYSLTPSSRFLLH 60
           M+LSELVE LHIQPSK+ CLG LMRLL HSGFFSQ   +N Q    +KY LTPSSRFLL 
Sbjct: 48  MTLSELVEALHIQPSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLS 107

Query: 61  HD------TTLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIA 120
            +      TTL+ LPF+FL LH+AMMAPWETMSSWL +AD    +AFE  +GKSIW+YIA
Sbjct: 108 PNMTTSTTTTLQALPFVFLALHQAMMAPWETMSSWLRTAD---GSAFETVHGKSIWNYIA 167

Query: 121 EEPDFGNLFHQTMEYDSQLIRRLVTR-GCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHI 180
           +EPD  NLFHQ++ YDS LI R+VTR  C +VFEG+KS+VDV G  GI+A AI EAFPHI
Sbjct: 168 DEPDLRNLFHQSVAYDSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHI 227

Query: 181 TCTAFDLQQMVSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIY 240
           TCT  DL Q  +             +G     +IPPTDA+LL                  
Sbjct: 228 TCTVLDLPQQTT-------------KGLNLAIQIPPTDAVLL------------------ 287

Query: 241 RILRDFGNQSVLHKWNDEESVRILKNCKDAIRGAKGGKVIIIEAVLENLMEDKESTETQL 300
                   +SVLH+ NDEES++ILK CKDAI   KGGKVIIIEAVLE  MED+ESTET+L
Sbjct: 288 --------KSVLHQCNDEESIQILKKCKDAISKGKGGKVIIIEAVLERQMEDEESTETEL 345

Query: 301 CGDVLMMASFTGRKRNEREWKSLFVAAGFSDYKITSFLGL 331
           CG+ LMMA+F   KRNEREWK LF+AAGFS+YKIT+FLGL
Sbjct: 348 CGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGL 345

BLAST of Spg030842 vs. NCBI nr
Match: KAG6581634.1 (putative O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 365.5 bits (937), Expect = 6.4e-97
Identity = 200/340 (58.82%), Postives = 235/340 (69.12%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFFSQIHGENEQQ---LKYSLTPSSRFLLH 60
           MSLSELVE LHIQPSK+ CLG LMRLL HSGFFSQ   +N Q    +KY LTPSSRFLL 
Sbjct: 49  MSLSELVEALHIQPSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLS 108

Query: 61  HD------TTLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIA 120
            +      TTL+ LPF+FL LH+AMMAPWETMSSWL +AD    +AFE  +GKSIW+YIA
Sbjct: 109 PNTTTSTTTTLQALPFVFLALHQAMMAPWETMSSWLRTAD---GSAFETVHGKSIWNYIA 168

Query: 121 EEPDFGNLFHQTMEYDSQLIRRLVTR-GCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHI 180
           +EPD  NLFHQ++ YDS LI R+VTR  C +VFEG+KS+VDV    GI+A AI EAFPHI
Sbjct: 169 DEPDLRNLFHQSVAYDSHLIGRIVTRPECRDVFEGVKSLVDVASDRGIIANAIAEAFPHI 228

Query: 181 TCTAFDLQQMVSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIY 240
           TCT  DL Q  +             +G     +IPPTDA+LL                  
Sbjct: 229 TCTVLDLPQQTT-------------KGLNLAIQIPPTDAVLL------------------ 288

Query: 241 RILRDFGNQSVLHKWNDEESVRILKNCKDAIRGAKGGKVIIIEAVLENLMEDKESTETQL 300
                   +SVLH+ NDEE ++ILK CKDAI  AKGGKV+IIEAVLE  MED+ESTET+L
Sbjct: 289 --------KSVLHQCNDEEGIQILKKCKDAISSAKGGKVVIIEAVLETQMEDEESTETEL 346

Query: 301 CGDVLMMASFTGRKRNEREWKSLFVAAGFSDYKITSFLGL 331
           CG+ LMMA+F   KRNEREWK LF+AAGFS+YKIT+FLGL
Sbjct: 349 CGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGL 346

BLAST of Spg030842 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.1e-66
Identity = 147/338 (43.49%), Postives = 198/338 (58.58%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFFSQIHGE-----NEQQLKYSLTPSSRFL 60
           M++S+L   L I   KS C+ RLMR+L+HSGFF+    E       ++  Y +T +S+ L
Sbjct: 65  MTISQLTLALPINRKKSPCVYRLMRILIHSGFFALQKAEVGEEGGGEEEGYVITDASKLL 124

Query: 61  LHHDTTLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIAEEPD 120
           L  D  +   PFL  +L   M  PW+ +S+W  + D    T F+ ANG + WDY + EP 
Sbjct: 125 L-KDNPMSVTPFLLAMLDPVMTKPWDFLSNWFQNGD---PTPFDTANGMAFWDYGSHEPK 184

Query: 121 FGNLFHQTMEYDSQLIRRLVTRGCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHITCTAF 180
               F+  M  D++L+  +V   C  VFEG++S+VDVGGG G +A +I  AFPHI CT F
Sbjct: 185 LARFFNDAMASDARLVTSVVIEKCKGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVF 244

Query: 181 DLQQMVSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIYRILRD 240
           DL  +V++ Q    ++LNF+ GD+F   +P T+ +LL                       
Sbjct: 245 DLPHVVADLQ--GGNNLNFVGGDMF-VDVPATEVVLL----------------------- 304

Query: 241 FGNQSVLHKWNDEESVRILKNCKDAI--RGAKGGKVIIIEAVLENLM-EDKESTETQLCG 300
              + +LH WNDEESV+ILK CK+AI     KGGKVIII+  +EN   ED ES ETQL  
Sbjct: 305 ---KWILHDWNDEESVKILKKCKEAISKSNKKGGKVIIIDMKVENEKDEDDESYETQLFF 364

Query: 301 DVLMMASFTGRKRNEREWKSLFVAAGFSDYKITSFLGL 331
           D+LMMA  TGR+RNE+EW  LF  AGFS+YKIT  LGL
Sbjct: 365 DMLMMALVTGRERNEKEWAKLFKDAGFSNYKITPILGL 369

BLAST of Spg030842 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 239.2 bits (609), Expect = 9.1e-62
Identity = 140/332 (42.17%), Postives = 192/332 (57.83%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFF-SQIHGENEQQLKYSLTPSSRFLLHHD 60
           M+L ELV  L + P +SQC+ RLMR+LVHSGF  +Q   + +++  Y LT +SR LL  D
Sbjct: 51  MTLPELVAKLPVHPKRSQCVYRLMRILVHSGFLAAQRVQQGKEEEGYVLTDASRLLL-MD 110

Query: 61  TTLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIAEEPDFGNL 120
            +L   P +  +L   +  PW  +S+W  + D    T F  A  +S WDY   EP   N 
Sbjct: 111 DSLSIRPLVLAMLDPILTKPWHYLSAWFQNDD---PTPFHTAYERSFWDYAGHEPQLNNS 170

Query: 121 FHQTMEYDSQLIRRLVTRGCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHITCTAFDLQQ 180
           F++ M  D++L+  ++ +    VF GL S+VDVGGG G +AKAI  AFPH+ CT  DL  
Sbjct: 171 FNEAMASDARLLTSVLLKEGQGVFAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSH 230

Query: 181 MVSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIYRILRDFGNQ 240
           +V+  Q     +LN+  GD+FEA IPP DAILL                          +
Sbjct: 231 VVAGLQ--GSKNLNYFAGDMFEA-IPPADAILL--------------------------K 290

Query: 241 SVLHKWNDEESVRILKNCKDAIRGAK-GGKVIIIEAVLENLMEDKESTETQLCGDVLMMA 300
            +LH W++EE V+ILK C++AI   + GGKVIII+ ++     D +STETQL  D+ MM 
Sbjct: 291 WILHDWSNEECVKILKRCREAIPSKENGGKVIIIDMIMMKNQGDYKSTETQLFFDMTMMI 349

Query: 301 SFTGRKRNEREWKSLFVAAGFSDYKITSFLGL 331
              GR+R+E EW+ LF+ AGFS YKIT  LGL
Sbjct: 351 FAPGRERDENEWEKLFLDAGFSHYKITPILGL 349

BLAST of Spg030842 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.6e-58
Identity = 135/334 (40.42%), Postives = 194/334 (58.08%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFF--SQIHGENEQQLKYSLTPSSRFLLHH 60
           ++LSELV+ L I  +KS CL RL+R+LVHSGFF  ++I  E +++  Y  T SS+ LL +
Sbjct: 64  VTLSELVQALQIPQAKSACLYRLLRILVHSGFFAITKIQSEGDEE-GYLPTLSSKLLLKN 123

Query: 61  DTTLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIAEEPDFGN 120
              +   P L  +++  M+AP    S W   +DD   T FE  +G S+W Y  E P    
Sbjct: 124 H-PMSMSPCLLGLVNPTMVAPMHFFSDWFKRSDDM--TPFEATHGASLWKYFGETPHMAE 183

Query: 121 LFHQTMEYDSQLIRRLVTRGCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHITCTAFDLQ 180
           +F++ M  +++L   +V + C    EG+ S+VDVGGG G + +AI EAFP++ CT  DL 
Sbjct: 184 IFNEAMGCETRLAMSVVLKECKGKLEGISSLVDVGGGTGNVGRAIAEAFPNVKCTVLDLP 243

Query: 181 QMVSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIYRILRDFGN 240
           Q+V N +    ++L F+ GD+F+  IPP D + L                          
Sbjct: 244 QVVGNLK--GSNNLEFVSGDMFQF-IPPADVVFL-------------------------- 303

Query: 241 QSVLHKWNDEESVRILKNCKDAIRG-AKGGKVIIIEAVLENLMEDK-ESTETQLCGDVLM 300
           + +LH WNDEE ++ILK CK+AI    +GGK+III+ V+ +  +   ESTETQL  D+ +
Sbjct: 304 KWILHDWNDEECIKILKRCKEAIPSKEEGGKLIIIDMVVNDHNKGSYESTETQLFYDLTL 363

Query: 301 MASFTGRKRNEREWKSLFVAAGFSDYKITSFLGL 331
           MA  TG +R E EWK LFVAAGF+ Y I+  LGL
Sbjct: 364 MALLTGTERTETEWKKLFVAAGFTSYIISPVLGL 364

BLAST of Spg030842 vs. ExPASy Swiss-Prot
Match: B0EXJ8 (Tabersonine 16-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=16OMT PE=1 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 6.1e-58
Identity = 134/326 (41.10%), Postives = 190/326 (58.28%), Query Frame = 0

Query: 1   MSLSELVEVL--HIQPSKSQCLGRLMRLLVHSGFFSQIHGENEQQLKYSLTPSSRFLLHH 60
           ++LSEL   L   + PSK+  + RLMR+L  +GF S+   + E +  YSLTPSSR LL  
Sbjct: 47  ITLSELTNALVPPVHPSKAPFIYRLMRVLAKNGFCSEEQLDGETEPLYSLTPSSRILLKK 106

Query: 61  DTTLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIAEEPDFGN 120
           +  L     +  +     +  WE++S W  + DD  STAFE A+GK+ W Y +E  +   
Sbjct: 107 E-PLNLRGIVLTMADPVQLKAWESLSDWYQNEDDS-STAFETAHGKNFWGYSSEHMEHAE 166

Query: 121 LFHQTMEYDSQLIRRLVTRGCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHITCTAFDLQ 180
            F++ M  DSQLI +L+      +FEGL S+VD+GGG G +AKAI + FP + CT FDL 
Sbjct: 167 FFNEAMASDSQLISKLLIGEYKFLFEGLASLVDIGGGTGTIAKAIAKNFPQLKCTVFDLP 226

Query: 181 QMVSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIYRILRDFGN 240
            +V+N +  SK+++ F+ GD+FE KIP  +AI L                          
Sbjct: 227 HVVANLE--SKENVEFVAGDMFE-KIPSANAIFL-------------------------- 286

Query: 241 QSVLHKWNDEESVRILKNCKDAIRGAKGGKVIIIEAVLENLMEDKESTETQLCGDVLMMA 300
           + +LH WNDE+ V+ILK+CK AI  AKGGKVIII+ V+ +  +D    +TQ   D+ M+ 
Sbjct: 287 KWILHDWNDEDCVKILKSCKKAI-PAKGGKVIIIDMVMYSDKKDDHLVKTQTSMDMAMLV 340

Query: 301 SFTGRKRNEREWKSLFVAAGFSDYKI 325
           +F  ++R E+EW  LF  AGFSDYKI
Sbjct: 347 NFAAKERCEKEWAFLFKEAGFSDYKI 340

BLAST of Spg030842 vs. ExPASy Swiss-Prot
Match: Q93WU2 (Eugenol O-methyltransferase OS=Ocimum basilicum OX=39350 GN=EOMT1 PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.8e-57
Identity = 131/330 (39.70%), Postives = 185/330 (56.06%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFFSQIHGENEQQLKYSLTPSSRFLLHHDT 60
           M+LS+L++ + I   K+QC  RLMR LV+S FF + +  N Q++ Y LTP+S  LL  + 
Sbjct: 54  MTLSQLLQSIPINKEKTQCFQRLMRALVNSNFFIEENNSNNQEVCYWLTPASCLLL-KEA 113

Query: 61  TLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIAEEPDFGNLF 120
            L   P + +VL      PW  MS W     ++ +T FE ANG + W+ +A EP  G  F
Sbjct: 114 PLTVTPLVQVVLDPTFTNPWHHMSEWF--THEKHATQFEAANGCTFWEKLANEPSKGRFF 173

Query: 121 HQTMEYDSQLIRRLVTRGCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHITCTAFDLQQM 180
            + M  DS+LI  + T+    V EG++++VDVGGG G MAKAI+EA P I CT  DL  +
Sbjct: 174 DEAMSCDSRLIAHVFTKDYKHVIEGIRTLVDVGGGNGTMAKAIVEAMPTIKCTVIDLPHV 233

Query: 181 VSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIYRILRDFGNQS 240
           V+  +  S D+LN+I GD+F++ IP  DAILL                          +S
Sbjct: 234 VAGLE--STDNLNYIGGDMFQS-IPSADAILL--------------------------KS 293

Query: 241 VLHKWNDEESVRILKNCKDAIRGAKGGKVIIIEAVLENLMEDKESTETQLCGDVLMMASF 300
           ++H W+D E ++ILK CKDA+    GGKVIII+ V+    +  E  E QL  D+ MM  F
Sbjct: 294 IIHDWDDVEGLKILKKCKDAV--VMGGKVIIIDVVVGVNHDIDEVLEDQLHFDMAMMCYF 349

Query: 301 TGRKRNEREWKSLFVAAGFSDYKITSFLGL 331
             ++R   EW+ L   AGF  YK+T   G+
Sbjct: 354 NAKERTMSEWEKLIYDAGFKSYKLTPAFGV 349

BLAST of Spg030842 vs. ExPASy TrEMBL
Match: A0A6J1DFY3 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111020151 PE=3 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 2.4e-121
Identity = 228/332 (68.67%), Postives = 264/332 (79.52%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFFSQIHGEN-EQQLKYSLTPSSRFLLHHD 60
           MSLS+LVE LHIQPSK+QCLGRLMRLLVHSGFF++ H EN EQ+ KY++TPSSR LL H+
Sbjct: 46  MSLSDLVEALHIQPSKAQCLGRLMRLLVHSGFFARTHSENDEQETKYAVTPSSRLLLRHN 105

Query: 61  TTLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIAEEPDFGNL 120
            TLETLPFLFL+LH AMMA WETMS WLCS D+  S+AFEMANGKSIW+YIAEEP+FGNL
Sbjct: 106 DTLETLPFLFLILHHAMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNL 165

Query: 121 FHQTMEYDSQLIRRLVTRGCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHITCTAFDLQQ 180
           FH+T+ YDSQLI R+VT  C EVFEGLKS+VDVGGG GIMAKAI+EAFPHIT   FDL Q
Sbjct: 166 FHRTIAYDSQLIGRIVTTECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQ 225

Query: 181 MVSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIYRILRDFGNQ 240
           +V+NHQ+ +K +L+FI GD+FEAKIPPT+A+LL                          +
Sbjct: 226 VVANHQQTTK-NLSFIGGDMFEAKIPPTNAVLL--------------------------K 285

Query: 241 SVLHKWNDEESVRILKNCKDAI-RGAKGGKVIIIEAVLENLMEDKESTETQLCGDVLMMA 300
           SVL+ WNDEES++ILKNCKDAI   A+GGKVIIIE  LE  +EDKES ETQLCGDVLMM 
Sbjct: 286 SVLYNWNDEESIQILKNCKDAILSNAEGGKVIIIEVALEYQVEDKESIETQLCGDVLMMT 345

Query: 301 SFTGRKRNEREWKSLFVAAGFSDYKITSFLGL 331
           +F  RKRNEREWK+LF+AAGFSDYKI   LGL
Sbjct: 346 NFAIRKRNEREWKNLFLAAGFSDYKIIPMLGL 350

BLAST of Spg030842 vs. ExPASy TrEMBL
Match: A0A6J1E096 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111025195 PE=3 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 1.5e-112
Identity = 209/331 (63.14%), Postives = 255/331 (77.04%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFFSQIHGENEQQLKYSLTPSSRFLLHHDT 60
           MSLS LV+ LHIQPSK+QCL RLMRLLVH+GFF Q HG+NEQ+ +Y+LTPSSR LL  +T
Sbjct: 46  MSLSNLVDALHIQPSKAQCLNRLMRLLVHAGFFIQTHGDNEQE-EYALTPSSRLLLCRNT 105

Query: 61  TLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIAEEPDFGNLF 120
             E +PF+FL+L++AMMAP ETMSSWLCS D+  ++AFEMANGKSIWDY+++E DFG LF
Sbjct: 106 AFENIPFVFLILNRAMMAPLETMSSWLCSVDEHSNSAFEMANGKSIWDYVSQEHDFGKLF 165

Query: 121 HQTMEYDSQLIRRLVTRGCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHITCTAFDLQQM 180
           HQTM +DSQLI ++VTR C EVFEGLKS+VDVGG  G MAKAI EAFPHITCT FDL Q+
Sbjct: 166 HQTMVHDSQLIGKVVTRECREVFEGLKSLVDVGGSTGFMAKAIAEAFPHITCTVFDLPQV 225

Query: 181 VSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIYRILRDFGNQS 240
            +N Q+ +K +L+FI GD+FEAKIPP +A+LL                          + 
Sbjct: 226 AANSQQTTK-NLSFIGGDMFEAKIPPANAVLL--------------------------KW 285

Query: 241 VLHKWNDEESVRILKNCKDAIRG-AKGGKVIIIEAVLENLMEDKESTETQLCGDVLMMAS 300
           +LH WNDEES+RILKNCKDAI   A+ GKVI+I+ V+EN +EDKES E QLC D+L+M S
Sbjct: 286 ILHDWNDEESIRILKNCKDAIPSRAEXGKVIVIDMVVENQIEDKESVEAQLCCDLLVMTS 345

Query: 301 FTGRKRNEREWKSLFVAAGFSDYKITSFLGL 331
           + GR+RNEREWK+LF+ AGFSDYKI S LGL
Sbjct: 346 YNGRERNEREWKNLFLTAGFSDYKIISMLGL 348

BLAST of Spg030842 vs. ExPASy TrEMBL
Match: A0A6J1DHJ0 (trans-resveratrol di-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111020124 PE=3 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 1.4e-102
Identity = 198/331 (59.82%), Postives = 238/331 (71.90%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFFSQIHGENEQQLKYSLTPSSRFLLHHDT 60
           MSLS LV  LHIQP K+ CLGRLM LLVHSGFF Q H ENEQ++KY+LT +SR LL H+T
Sbjct: 46  MSLSNLVGALHIQPFKAHCLGRLMSLLVHSGFFVQTHDENEQEVKYTLTSTSRLLLRHNT 105

Query: 61  TLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIAEEPDFGNLF 120
             E +  L L+ +KAMMA +ETMSSW  S D++ S AFE  N KS+WDY+A+EPDF N+ 
Sbjct: 106 PFEHISLLLLIFNKAMMASYETMSSWFRSTDERHSCAFETTNDKSVWDYVAQEPDFSNIL 165

Query: 121 HQTMEYDSQLIRRLVTRGCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHITCTAFDLQQM 180
           ++ M YDSQLI R+VTR C  VF+GLKS+VDVGG  G MAKAI EAFPHITC  FDL  +
Sbjct: 166 NKAMMYDSQLIGRVVTRECRRVFKGLKSLVDVGGNRGFMAKAIAEAFPHITCIVFDLPHV 225

Query: 181 VSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIYRILRDFGNQS 240
           V N Q+ + ++LNFI G++FEAKIPP +A+LL                          + 
Sbjct: 226 VDNLQQ-TTENLNFIAGNMFEAKIPPANAVLL--------------------------KW 285

Query: 241 VLHKWNDEESVRILKNCKDAIRG-AKGGKVIIIEAVLENLMEDKESTETQLCGDVLMMAS 300
           VLH WNDE+S++ILKNCKDAI   A+GGKVIIIE V EN +EDKES ETQLC DV  M+ 
Sbjct: 286 VLHDWNDEKSIKILKNCKDAIPSRAEGGKVIIIEMVTENQIEDKESIETQLCEDVFTMSV 345

Query: 301 FTGRKRNEREWKSLFVAAGFSDYKITSFLGL 331
            TGR+RNE EWK+LF+AAGFSDYKI S LGL
Sbjct: 346 VTGRERNESEWKNLFLAAGFSDYKIISMLGL 349

BLAST of Spg030842 vs. ExPASy TrEMBL
Match: A0A6J1IXH8 (probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111481540 PE=3 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 4.0e-97
Identity = 200/341 (58.65%), Postives = 234/341 (68.62%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFFSQIHGENEQQ---LKYSLTPSSRFLLH 60
           MSLSELVE LHIQPSK+ CLG LMRLL HSGFFSQ   +N Q    +KY LTPSSRFLL 
Sbjct: 49  MSLSELVEALHIQPSKAHCLGVLMRLLHHSGFFSQTQDQNHQPQEVVKYGLTPSSRFLLR 108

Query: 61  HD-------TTLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYI 120
            +       TTL+ LPF+FL LH+AMMAPWETMSSWL +AD    +AFE  +GKSIW+YI
Sbjct: 109 PNTTTSTTTTTLQALPFVFLALHQAMMAPWETMSSWLRTAD---GSAFETLHGKSIWNYI 168

Query: 121 AEEPDFGNLFHQTMEYDSQLIRRLVTR-GCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPH 180
           A+EPD  NLFHQ++ YDS LI R+VTR  C +VFEG+KS+VDV G  GI+A AI EAFPH
Sbjct: 169 ADEPDLRNLFHQSVAYDSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPH 228

Query: 181 ITCTAFDLQQMVSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVI 240
           ITCT  DL Q  +             +G     +IPPTDA+LL                 
Sbjct: 229 ITCTVLDLPQQTT-------------KGLNLAIQIPPTDAVLL----------------- 288

Query: 241 YRILRDFGNQSVLHKWNDEESVRILKNCKDAIRGAKGGKVIIIEAVLENLMEDKESTETQ 300
                    +SVLH+ NDEE ++ILK CKDAI   KGGKV+IIEAVLE  MED ESTET+
Sbjct: 289 ---------KSVLHQCNDEEGIQILKKCKDAISSVKGGKVVIIEAVLETQMEDAESTETE 347

Query: 301 LCGDVLMMASFTGRKRNEREWKSLFVAAGFSDYKITSFLGL 331
           LCG+ LMMA+F   KRNEREWK LF+AAGFS+YKIT+FLGL
Sbjct: 349 LCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGL 347

BLAST of Spg030842 vs. ExPASy TrEMBL
Match: A0A6J1F418 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111442193 PE=3 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 3.4e-96
Identity = 197/340 (57.94%), Postives = 235/340 (69.12%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFFSQIHGENEQQ---LKYSLTPSSRFLLH 60
           +SLSELVE LHIQPSK+ CLG LMRLL HSGFFSQ   +N Q    +KY LTPSSRFLL 
Sbjct: 49  ISLSELVEALHIQPSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLS 108

Query: 61  HD------TTLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIA 120
            +      TTL+ LPF+FL LH+AMMAPWETMSSWL +AD    +AFE  +GKSIW+Y+A
Sbjct: 109 PNTTTSTTTTLQALPFVFLALHQAMMAPWETMSSWLRTAD---GSAFETVHGKSIWNYVA 168

Query: 121 EEPDFGNLFHQTMEYDSQLIRRLVTR-GCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHI 180
           +EPD  NLFHQ++ YDS LI R+VTR  C +VFEG+KS+VDV    GI+A AI EAFPHI
Sbjct: 169 DEPDLRNLFHQSVAYDSHLIGRIVTRPECRDVFEGVKSLVDVASDRGIIANAIAEAFPHI 228

Query: 181 TCTAFDLQQMVSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIY 240
           TCT  DL Q  +             +G     +IPPTDA+LL                  
Sbjct: 229 TCTVLDLPQQTT-------------KGLNLAIQIPPTDAVLL------------------ 288

Query: 241 RILRDFGNQSVLHKWNDEESVRILKNCKDAIRGAKGGKVIIIEAVLENLMEDKESTETQL 300
                   +SVLH+ NDEE ++ILK CKDAI  AKGGKV+IIEAVLE  MED+ESTET+L
Sbjct: 289 --------KSVLHQCNDEEGIQILKKCKDAISSAKGGKVVIIEAVLETQMEDEESTETEL 346

Query: 301 CGDVLMMASFTGRKRNEREWKSLFVAAGFSDYKITSFLGL 331
           CG+ LMMA+F   KRNEREWK LF+AAGFS+YKI++FLGL
Sbjct: 349 CGNALMMATFNNTKRNEREWKDLFMAAGFSNYKISTFLGL 346

BLAST of Spg030842 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 144.4 bits (363), Expect = 2.2e-34
Identity = 101/334 (30.24%), Postives = 170/334 (50.90%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFFSQIHGENEQQLKYSLTPSSRFLLHHDT 60
           ++L+EL   +   PS    L R+MR LVH G F +I  ++     Y  TP SR L+    
Sbjct: 67  VTLAELSSAVSASPSH---LRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRR 126

Query: 61  TLETL-PFLFLVLHKAMMAPWETMSSWLCS-ADDQCSTAFEMANGKSIWDYIAEEPDFGN 120
             ++L PF+       M+APW  +SS + S  +      F+  +GK +W +  + P   +
Sbjct: 127 DGKSLAPFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSD 186

Query: 121 LFHQTMEYDSQLIRRLVTRGCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHITCTAFDLQ 180
           + ++ M  D++ +   V   C  +F+G+ +MVDVGGG G     +++ FP I    FDL 
Sbjct: 187 MINEAMACDARRVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLP 246

Query: 181 QMVSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIYRILRDFGN 240
            ++   + +  D +  +EGD+F++ IP  DAI +                          
Sbjct: 247 HVIEVAEVL--DGVENVEGDMFDS-IPACDAIFI-------------------------- 306

Query: 241 QSVLHKWNDEESVRILKNCKDAIRGAKGGKVIIIEAVL-ENLM------EDKESTETQLC 300
           + VLH W D++ ++ILKNCK+A+     GKV+I+E+V+ EN         D++    +L 
Sbjct: 307 KWVLHDWGDKDCIKILKNCKEAV-PPNIGKVLIVESVIGENKKTMIVDERDEKLEHVRLM 366

Query: 301 GDVLMMA-SFTGRKRNEREWKSLFVAAGFSDYKI 325
            D++MMA + TG++R  +EW  +   AGF+ Y++
Sbjct: 367 LDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEV 367

BLAST of Spg030842 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 116.7 bits (291), Expect = 4.8e-26
Identity = 88/335 (26.27%), Postives = 151/335 (45.07%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQCLGRLMRLLVHSGFFSQIHGENEQQLKYSLTPSSRFLLHHDT 60
           ++LSEL   +   PS    L R+MR LVH G F ++  ++     Y+ TP SR ++    
Sbjct: 53  VTLSELSSAVSASPSH---LRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMM---- 112

Query: 61  TLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIAEEPDFGNLF 120
                                                    +GK +W +  +      L 
Sbjct: 113 -------------------------------------ITKLHGKDLWAFAQDNLCHSQLI 172

Query: 121 HQTMEYDSQLIRRLVTRGCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHITCTAFDLQQM 180
           ++ M  D++ +   V   C  +F+G+ ++VDVGGG G     +++ FP I    FDL  +
Sbjct: 173 NEAMACDARRVVPRVAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHV 232

Query: 181 VSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIYRILRDFGNQS 240
           +   Q +  D +  +EGD+F++ IP +DA+++                          + 
Sbjct: 233 IEVAQVL--DGVENVEGDMFDS-IPASDAVII--------------------------KW 292

Query: 241 VLHKWNDEESVRILKNCKDAIRGAKGGKVIIIEAVL---ENLM----EDKESTETQLCGD 300
           VLH W D++ ++ILKNCK+A+     GKV+I+E V+   +N M     D +    +L  D
Sbjct: 293 VLHDWGDKDCIKILKNCKEAVL-PNIGKVLIVECVIGEKKNTMIAEERDDKLEHVRLQLD 313

Query: 301 VLMMA-SFTGRKRNEREWKSLFVAAGFSDYKITSF 328
           ++MM  + TG++R  +EW  +   AGF+ Y++  F
Sbjct: 353 MVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDF 313

BLAST of Spg030842 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 106.7 bits (265), Expect = 5.0e-23
Identity = 93/337 (27.60%), Postives = 160/337 (47.48%), Query Frame = 0

Query: 1   MSLSELVEVLHIQPSKSQC---LGRLMRLLVHSGFF------SQIHGENEQ-QLKYSLTP 60
           +S SE+   L  +P+  +    L R++RLLV           S+ +G+  + +  Y+  P
Sbjct: 67  LSPSEIAVSLPNKPTNPEAPVLLDRMLRLLVSHSILKCCMVESRENGQTGKIERVYAAEP 126

Query: 61  SSRFLLHHDTTLETLPFLFLVLH-KAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDY 120
             ++ L       +L  L L+LH + ++  W  +   +    D    AF  A+   +++Y
Sbjct: 127 ICKYFLKDSDGSGSLSSLLLLLHSQVILKTWTNLKDVILEGKD----AFSSAHDMRLFEY 186

Query: 121 IAEEPDFGNLFHQTMEYDSQLIRRLVT---RGCPEVFEGLKSMVDVGGGAGIMAKAIMEA 180
           I+ +  F  LFH+ M   S ++ + V    RG    FE + ++VDVGGG G +   I   
Sbjct: 187 ISSDDQFSKLFHRAMSESSTMVMKKVLEEYRG----FEDVNTLVDVGGGIGTILGLITSK 246

Query: 181 FPHITCTAFDLQQMVSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLV 240
           +PHI    FDL Q+++  Q      +  + GD+F  ++P  DAI +              
Sbjct: 247 YPHIKGVNFDLAQVLT--QAPFYPGVKHVSGDMF-IEVPKGDAIFM-------------- 306

Query: 241 KVIYRILRDFGNQSVLHKWNDEESVRILKNCKDAIRGAKGGKVIIIEAVLENLMEDKE-- 300
                       + +LH W DE+ ++ILKNC  ++   + GKVII+E +    ME K   
Sbjct: 307 ------------KWILHDWGDEDCIKILKNCWKSL--PEKGKVIIVEMITP--MEPKPND 362

Query: 301 -STETQLCGDVLMMASFT-GRKRNEREWKSLFVAAGF 320
            S  T L  D+LM+   + G++R+  ++++L  A+GF
Sbjct: 367 FSCNTVLGMDLLMLTQCSGGKERSLSQFENLAFASGF 362

BLAST of Spg030842 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 105.9 bits (263), Expect = 8.5e-23
Identity = 83/330 (25.15%), Postives = 150/330 (45.45%), Query Frame = 0

Query: 1   MSLSELVEVLHIQ-PSKSQCLGRLMRLLVHSGFFS----QIHGENEQQLKYSLTPSSRFL 60
           MS +E+   L  + P     L R++RLL      +    ++ G+  +++ Y L P  ++L
Sbjct: 55  MSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRKLSGDGVERI-YGLGPVCKYL 114

Query: 61  LHHDTTLETLPFLFLVLHKAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIAEEPD 120
             ++  +       +   K +M  W  +   +          F  A G S ++Y   +P 
Sbjct: 115 TKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDG----GIPFNKAYGMSAFEYHGTDPR 174

Query: 121 FGNLFHQTMEYDSQLIRRLVTRGCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHITCTAF 180
           F  +F+  M   S +  + +     + FEGL S+VDVGGG G   K I+  +P++    F
Sbjct: 175 FNKVFNNGMSNHSTITMKKILE-TYKGFEGLTSLVDVGGGIGATLKMIVSKYPNLKGINF 234

Query: 181 DLQQMVSNHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIYRILRD 240
           DL  ++ +    S   +  + GD+F   +P  DAI +                       
Sbjct: 235 DLPHVIEDAP--SHPGIEHVGGDMF-VSVPKGDAIFM----------------------- 294

Query: 241 FGNQSVLHKWNDEESVRILKNCKDAIRGAKGGKVIIIEAVLENLMEDKESTETQLCGDVL 300
              + + H W+DE  V+ LKNC +++   + GKVI+ E +L    +   ST+  +  D +
Sbjct: 295 ---KWICHDWSDEHCVKFLKNCYESL--PEDGKVILAECILPETPDSSLSTKQVVHVDCI 347

Query: 301 MMA-SFTGRKRNEREWKSLFVAAGFSDYKI 325
           M+A +  G++R E+E+++L  A+GF   K+
Sbjct: 355 MLAHNPGGKERTEKEFEALAKASGFKGIKV 347

BLAST of Spg030842 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 102.4 bits (254), Expect = 9.4e-22
Identity = 88/319 (27.59%), Postives = 150/319 (47.02%), Query Frame = 0

Query: 14  PSKSQCLGRLMRLLV-HSGFFSQI--HGENEQQLK----YSLTPSSRFLLHHDTTLETLP 73
           P     L R++ LLV HS    ++   GEN +  K    Y+  P  ++ L       +L 
Sbjct: 83  PEAPVLLDRMLSLLVSHSILKCRMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGSLV 142

Query: 74  FLFLVLH-KAMMAPWETMSSWLCSADDQCSTAFEMANGKSIWDYIAEEPDFGNLFHQTME 133
            LF++LH +     W  +   +    D    AF  A+G  I++YI  +  F  LF++ M 
Sbjct: 143 PLFMLLHTQVFFKTWTNLKDVILEGRD----AFNSAHGMKIFEYINSDQPFAELFNRAMS 202

Query: 134 YDSQLIRRLVT---RGCPEVFEGLKSMVDVGGGAGIMAKAIMEAFPHITCTAFDLQQMVS 193
             S +I + V    RG    FE + ++VDVGGG G +   +   +PHI    FDL Q+++
Sbjct: 203 EPSTMIMKKVLDVYRG----FEDVNTLVDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLT 262

Query: 194 NHQKISKDHLNFIEGDIFEAKIPPTDAILLQVYMFLVYNFLRLVKVIYRILRDFGNQSVL 253
             Q      +  + GD+F  ++P  DA+ +                          + +L
Sbjct: 263 --QAPFYPGVEHVSGDMF-VEVPKGDAVFM--------------------------KWIL 322

Query: 254 HKWNDEESVRILKNCKDAIRGAKGGKVIIIEAVLENLMEDKE-STETQLCGDVLMMASFT 313
           H W DE+ ++ILKNC  ++   + GK+II+E V     +  + S+ T    D+LM+   +
Sbjct: 323 HDWGDEDCIKILKNCWKSL--PEKGKIIIVEFVTPKEPKGGDLSSNTVFAMDLLMLTQCS 362

Query: 314 -GRKRNEREWKSLFVAAGF 320
            G++R+  ++++L  A+GF
Sbjct: 383 GGKERSLSQFENLAFASGF 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152414.14.9e-12168.67probable O-methyltransferase 3 [Momordica charantia][more]
XP_022158734.13.2e-11263.14probable O-methyltransferase 3 [Momordica charantia][more]
XP_022152376.13.0e-10259.82trans-resveratrol di-O-methyltransferase-like [Momordica charantia][more]
XP_023520750.13.8e-9759.12probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo] >XP_023528579.1 prob... [more]
KAG6581634.16.4e-9758.82putative O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
B0ZB572.1e-6643.49Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1[more]
B6VJS49.1e-6242.17Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1... [more]
A0A0N9HMN61.6e-5840.42Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 P... [more]
B0EXJ86.1e-5841.10Tabersonine 16-O-methyltransferase OS=Catharanthus roseus OX=4058 GN=16OMT PE=1 ... [more]
Q93WU21.8e-5739.70Eugenol O-methyltransferase OS=Ocimum basilicum OX=39350 GN=EOMT1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DFY32.4e-12168.67probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111020151 PE... [more]
A0A6J1E0961.5e-11263.14probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111025195 PE... [more]
A0A6J1DHJ01.4e-10259.82trans-resveratrol di-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1IXH84.0e-9758.65probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111481540 PE=3 ... [more]
A0A6J1F4183.4e-9657.94probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111442193 PE=... [more]
Match NameE-valueIdentityDescription
AT4G35160.12.2e-3430.24O-methyltransferase family protein [more]
AT4G35150.14.8e-2626.27O-methyltransferase family protein [more]
AT1G77530.15.0e-2327.60O-methyltransferase family protein [more]
AT5G54160.18.5e-2325.15O-methyltransferase 1 [more]
AT1G77520.19.4e-2227.59O-methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 1..55
e-value: 3.9E-14
score: 54.1
IPR001077O-methyltransferase domainPFAMPF00891Methyltransf_2coord: 240..320
e-value: 3.1E-18
score: 65.9
coord: 80..216
e-value: 7.4E-26
score: 90.8
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 59..332
e-value: 5.7E-66
score: 224.2
NoneNo IPR availablePANTHERPTHR11746:SF250O-METHYLTRANSFERASEcoord: 1..214
coord: 240..329
IPR016461O-methyltransferase COMT-typePANTHERPTHR11746O-METHYLTRANSFERASEcoord: 1..214
coord: 240..329
IPR016461O-methyltransferase COMT-typePROSITEPS51683SAM_OMT_IIcoord: 1..343
score: 52.518471
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 65..235
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 240..329
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 1..58

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg030842.1Spg030842.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008171 O-methyltransferase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
molecular_function GO:0008168 methyltransferase activity