Spg030832 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg030832
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RING/FYVE/PHD zinc finger superfamily protein
Location	scaffold11: 25108220 .. 25112745 (+)
RNA-Seq Expression	Spg030832
Synteny	Spg030832

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATGAAGAGAACAAAACGCGAAATTGAAACATTGGCGAGAAAATGGAAACATCACTCGAGAAATTGATCAGATTCCGATTCGTTAAACGGCGAATTGAGGAGTCGTTTTGTGGAGGGACGTGGCTTCTTTGAATTCCAACTGGGCCCTCTTCTGCCGTTGGAATCTCTCGCATTTCTCCTTCTCCCCGCTACGTTCTAGAGAGAGAGAGAGAGAGAAAGCACAAACTTTGCTGCTCTTAATGCATTTTCTCTATAAATAATCTCTTGTTTGTTCAATTGGTTTGCTGTAATTATCAAAATTTCTGTCATCAAATGTAATGGAATTATGAGAACACTCCATCTCTTGCTTCCTCTTTGAAAAAACAGCAGCAGGAGGAAAAGAAGGCTGATATTGTCTGTTTCTTCTGCCGCCGAAATTTTATTCCCATTTTTGTAAGCATCTCTATTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTGCTTTTAATTTAACTTACATGATTTTCTGAAGTTTTTGCCTGCAATGTGTTTGTTCATTTGCCGATCATGGTGGTGGTTGTTTGAAGCTTGCTGCCATTGTCTCTTTTTTGTGCATTTTCGTTAATTTTAGACTTCTCTTGATCTGGGTTGCTGTTTTCTTGAATTTCTTTGGCTTTGGTTTCTGGGTTTTGTTTTTGTTTGGTTTTCCAATGTGGGTCTCTTCGTATGTTGCGTGTGTGTGTGTTTTTTAATCGATTATCCTTCTTGAGTTTTTATACCATTTGATCTTATGGTTTTAACTTGATGCATTGTTTGGTCTATGGTGTTTTTAGTGGTCATTTTTTCTTACATTTGTACATCCTTGAAATAGAGAATTTTATTGCATCTTTTGTTAGATTGCTGCCAAAACTTGAAGAAGCGAGCATGTTTGAAATCGTGCCTTCCTGCTCAGTCTTGAACTGTGAATGATGAATGCGTTTGAATCAGATGACTACTTTTTGAGTCTTAAGAACTAAATGTTTGAAAGAGAGCTAAATCTTACTGTTCTAGCCGCTCAATGCTCCTATTATGCTCATAGGTCTATTCCTTTCCTACTAATTCTCGAGGAGGCTTTGCCTATTCGGAGGCCAATCTCTAGAGAATATGTATTTTAAATTACTAAGGTCCCATTTGATAACAATTTTGTTTTTACTTTTTTTGTTTTTAAAAATTAGGCTTGTTTCCTCAATTTCTTTACAATGATTTTCTATTAGGTACCCAAACTAAGAAAACAGCAAAACACTAATATGTTGCAATAACAAAGAGGAATTACAATATAACAATAGCCTTTCGAGAGAGTTAACTCTCTCCCAAAGCCTCACTATGAGACTATACCCAAAATCATCCACCCCATCTAAAGACCCTAACTCCCCTATTTATAACCAAAACTCCTTAACTAATTACAACTATGCCCTCACTACTAATCTAATACTATTCCCCAAATATCCCTAACTAGTACTCTCACAATACTCCACCCTTCAAAGACGAACACTGAAAAGTTGAAAACACACTTAGATAATATATTGCAATATCAAAAGGAACTACAATATATCAATAGCCTTTCAAGAGGGTTATCTCTCTCCCAAGAGTCCCACAATGGACACTACTACTGAAATTCTCCACACCTCTACGGCTCCCTAACTTCCCTATTTATAACCAAGGCACCCTAGCTAATTACTATTATACCCTTTACTACCATTCTAATACTATTTCACAAATATCCCTAACTAGTACTCTCACAATACTTCTGTTAGGGACCAAGGCAGTATGGGATGTCTTTGTCCCACATTGGAATTATATGGGCTCCTAATGGGGTATATAAGTGGTTGGTTCTCCCTCCCCAATCGCCTGGCTTTTGGGGTGTGGCTCTCCAACTACTTAGATACCTAACAACTTCACCCTTCAAAGACGAACACTGAAAACACACTTAGATAATATATTGCAATATCAAAAGGAACTACTATCAATAGCCTTTTGAGAGGGCTATCTCTCTCCCAAGAGTCCCACAATGGACACTATTACTGAAATTCTCCACACCTCTACGGCACCCTAGCTAATTACTATTATACCCTTACTACCATTCTAATACTATTTCCCAAATATCCCTAACCAATACTCTCACATTTTCATCTTTATTCAAAACTTCGCTTGAATTTTGAAAACATTTATAAAAAGTAGATAACAAAACACAGAAACTTATGGATGGAAGTAGTGTTTATAAGCTTAATTTTCAAAAACCAAATGGTTACCAAACGGAACCTAAGTTTTCAACATGGGGTTTGAATCTAGAACTTTGGAAGGTACCACCACACATCTCGAGGCCCCATTATTTGAGATAACAAAACTGTACTTAAGCATTGACGTAACTTTTCAAGTATCAGTGAAAATGTTTTTTCCCCTAAAATAGACAACAAAAAAGGACAACTGTATGGAGTAATTGGTTTTTCATTTGTCCTAAAGTCTATAATACATGATCTTGCTTGTTTTTCAAACTAGGATCGATTTCTTTGATTAGAGGGATTTCTTTCATCTTGCAATCAGTTTTCTAATTTCTCCTCTATCTTAATTTTGGGAGAAAAGGAGGTAAATAAACATCTATAATTCTTTAGTTTTTATTTTTGGAAGTATCTATAGCATTATGCCCAAAATGAACTTCTCAAGTGAACTACACAGTTGCAACTGTTCTACAGTTGGTGAAGTTTCTAATAATTGATGCTCATAAAAGTGAAAATCTCCAAGGTCCCAATCTTTAGTTTCTTTTGTGTTATGGTACTTCCTTAAAAAGGAGATAGAGAATCTGAATATATACTTGCATATTTTTTTATGTCCACGACACTTTTTCTATTCAGATTTTTGTGCATTTTTCTTTTCTCATGCATCTGATGATTATTTATTTACAGATTACTTATCATTACATTATGTCTAATCTTCAGGTAAGTTTAAAGAATCTCTCTTCTTCTTTTTAATTGTGCTTTTTAAATTACTAGTGAATCAGGGATCTCATTCCTAGCATGGTCATGGCCCACCCCATTAATCTTTCTGAAGCCAAGACCTTGGATTATATCTCCAGACATCCCAATTCCAAAGCCCTTACGAGTTACCCCACCACTTTTGGGACGTAAATTCCAAAAATTACAAATTGTCTTTTCTTTCTTTCTTTTCTGCACTTTATCTTAGAAGTTATATCATGCTGAGAAACAATCTGAGTTCTCATGGAAGTTGATCACTGCAGCAAACAAAATTGATCTGCACTCTTGTAATTGTTGTTTGGCTTGTTCTATTTATTTTATTAACATTCATACCAATGTCTACTGGGTCATTGAAGAATGGGGGATCATTTTGTGCTATTGGTGAATCGGTTGCTCACTGAATCAAACCTCGAAGCTACTATTGAGAGAAAAAACAGAATTTGTCAGCCTATGGCCTCAACAAATGCAGATAATATGATATCTTCCGTAGATATAGATGTTGAATCCATATCACCTTCAAGTAAACTTGTTGAGTGCAGAATTTGTTATGATGAGGATGATGAATCAAATATGGAGACTCCGTGCTCTTGCTGTGGCAGCTTGAAGGTTAGATTTAGTTTAGTCAACACCAACTGGTTTCATTGATAAAACTCAGATAGCTATTCTTTGAATCTTCCTTTTCCAAATTCTATGGCTGACTAAACTGGTTTAACATTGTTCTTGTCAACATTTTCTGCATTTAGTTTAGTATCCAACTTTATCTTTTCCCCTCAAATCTTTGCCCAGGATGTTATAATATCAATCAGGTACCTCAGTTTATATTTAAATCCTGTGCTATTCTTGGTTTCCTTTCCCTTTCCAATCCTTATTCCTCATTGTGTTCGTTTAGATGGTTCACCTCGATAATTAGCTTCCAATTAGTGTTGATTATTGCAGTATGCTCATCGTAAATGCGTCCAGTGGTGGTGTGACGAGAAAGGCAATACAACTTGTGAGATTTGCCACCAGGTCTGAAAAAATCTCTTATACAGGGCTAAATTTGTGGTAGTAAGAAGTCTTTTTTTCTTATACTGCAGGACTTTAAGCCCGGGTATGCAGCACCTCCCCCTGTATTTTATTATGGTGGTATTAATTCACCAATACATTTCAGGTAAGCCATTTACCTCTCTTCCCCCAGTGCACCTGCAAGTTTCTTTAACTTATCTTCTATATTAAATTCTTGTTCATTCAGTTTCTTAACGCTATTGATTTAACGATGCTGTCATCAAATTGTGTGGTCTTAGAGGAAGTTGGGAGATGTCTAGACTGAAGCTGCATGTACCTGCAGGAATGCCTGATCACGAGTATCTCAATTCTGACTTTGATGATTTCCTCACTCCCTCTCCAAGAAGCACATTGTGCTGCTGCATTGTTGCAGTAATTGTATTCCCTATCTCTTCATTTTACTTCATTTGCAGTTTCCTTATCTCTTTAAACAAATTTTAATGCTCTCGGTATTTGGCTTTGCCTTTAGCAGATGGAAACGTATT

mRNA sequence

ATGGGGGATCATTTTGTGCTATTGGTGAATCGGTTGCTCACTGAATCAAACCTCGAAGCTACTATTGAGAGAAAAAACAGAATTTGTCAGCCTATGGCCTCAACAAATGCAGATAATATGATATCTTCCGTAGATATAGATGTTGAATCCATATCACCTTCAAGTAAACTTGTTGAGTGCAGAATTTGTTATGATGAGGATGATGAATCAAATATGGAGACTCCGTGCTCTTGCTGTGGCAGCTTGAAGTATGCTCATCGTAAATGCGTCCAGTGGTGGTGTGACGAGAAAGGCAATACAACTTGTGAGATTTGCCACCAGGACTTTAAGCCCGGGTATGCAGCACCTCCCCCTGTATTTTATTATGGTGGTATTAATTCACCAATACATTTCAGAGGAAGTTGGGAGATGTCTAGACTGAAGCTGCATGTACCTGCAGGAATGCCTGATCACGAGTATCTCAATTCTGACTTTGATGATTTCCTCACTCCCTCTCCAAGAAGCACATTGTGCTGCTGCATTGTTGCAGTAATTGTATTCCCTATCTCTTCATTTTACTTCATTTGCAGTTTCCTTATCTCTTTAAACAAATTTTAA

Coding sequence (CDS)

ATGGGGGATCATTTTGTGCTATTGGTGAATCGGTTGCTCACTGAATCAAACCTCGAAGCTACTATTGAGAGAAAAAACAGAATTTGTCAGCCTATGGCCTCAACAAATGCAGATAATATGATATCTTCCGTAGATATAGATGTTGAATCCATATCACCTTCAAGTAAACTTGTTGAGTGCAGAATTTGTTATGATGAGGATGATGAATCAAATATGGAGACTCCGTGCTCTTGCTGTGGCAGCTTGAAGTATGCTCATCGTAAATGCGTCCAGTGGTGGTGTGACGAGAAAGGCAATACAACTTGTGAGATTTGCCACCAGGACTTTAAGCCCGGGTATGCAGCACCTCCCCCTGTATTTTATTATGGTGGTATTAATTCACCAATACATTTCAGAGGAAGTTGGGAGATGTCTAGACTGAAGCTGCATGTACCTGCAGGAATGCCTGATCACGAGTATCTCAATTCTGACTTTGATGATTTCCTCACTCCCTCTCCAAGAAGCACATTGTGCTGCTGCATTGTTGCAGTAATTGTATTCCCTATCTCTTCATTTTACTTCATTTGCAGTTTCCTTATCTCTTTAAACAAATTTTAA

Protein sequence

MGDHFVLLVNRLLTESNLEATIERKNRICQPMASTNADNMISSVDIDVESISPSSKLVECRICYDEDDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDFKPGYAAPPPVFYYGGINSPIHFRGSWEMSRLKLHVPAGMPDHEYLNSDFDDFLTPSPRSTLCCCIVAVIVFPISSFYFICSFLISLNKF

Homology

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BLAST of Spg030832 vs. NCBI nr
Match: XP_031738305.1 (E3 ubiquitin-protein ligase MARCH2 isoform X1 [Cucumis sativus])

HSP 1 Score: 321.2 bits (822), Expect = 6.1e-84
Identity = 150/180 (83.33%), Postives = 163/180 (90.56%), Query Frame = 0


Query: 1   MGDHFVLLVNRLLTESNLEATIERKNRICQPMAST--NADNMISSVDIDVESISPSSKLV 60
           MGDHF LLV++LLTESNLEATIERKNRIC PMAST  NADNMISS +IDVES+SPSS +V
Sbjct: 56  MGDHFGLLVDQLLTESNLEATIERKNRICHPMASTNANADNMISSSNIDVESVSPSS-IV 115

Query: 61  ECRICYDEDDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDFKPGYAAPPP 120
           +CRIC+DEDD S METPCSCCGSLKYAHRKC+Q WC+EKG+T CEICHQDFKPGY +PPP
Sbjct: 116 QCRICHDEDDGSKMETPCSCCGSLKYAHRKCIQRWCNEKGDTICEICHQDFKPGYTSPPP 175

Query: 121 VFYYGGINSPIHFRGSWEMSRLKLHVPAGMPDHEYLNSDFDDFLTPSPRSTLCCCIVAVI 179
           VFYYG INSPIHFRGSWEMSRL LHVPAGMPDHEYL+SDFDDF  PSPRS LCC +VAVI
Sbjct: 176 VFYYGDINSPIHFRGSWEMSRLNLHVPAGMPDHEYLDSDFDDFFAPSPRSILCCRVVAVI 234

[Close]

BLAST of Spg030832 vs. NCBI nr
Match: XP_011651201.1 (E3 ubiquitin-protein ligase MARCH2 isoform X2 [Cucumis sativus] >XP_011651203.1 E3 ubiquitin-protein ligase MARCH2 isoform X2 [Cucumis sativus] >XP_031738306.1 E3 ubiquitin-protein ligase MARCH2 isoform X2 [Cucumis sativus] >KGN57446.1 hypothetical protein Csa_011549 [Cucumis sativus])

HSP 1 Score: 321.2 bits (822), Expect = 6.1e-84
Identity = 150/180 (83.33%), Postives = 163/180 (90.56%), Query Frame = 0


Query: 1   MGDHFVLLVNRLLTESNLEATIERKNRICQPMAST--NADNMISSVDIDVESISPSSKLV 60
           MGDHF LLV++LLTESNLEATIERKNRIC PMAST  NADNMISS +IDVES+SPSS +V
Sbjct: 1   MGDHFGLLVDQLLTESNLEATIERKNRICHPMASTNANADNMISSSNIDVESVSPSS-IV 60

Query: 61  ECRICYDEDDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDFKPGYAAPPP 120
           +CRIC+DEDD S METPCSCCGSLKYAHRKC+Q WC+EKG+T CEICHQDFKPGY +PPP
Sbjct: 61  QCRICHDEDDGSKMETPCSCCGSLKYAHRKCIQRWCNEKGDTICEICHQDFKPGYTSPPP 120

Query: 121 VFYYGGINSPIHFRGSWEMSRLKLHVPAGMPDHEYLNSDFDDFLTPSPRSTLCCCIVAVI 179
           VFYYG INSPIHFRGSWEMSRL LHVPAGMPDHEYL+SDFDDF  PSPRS LCC +VAVI
Sbjct: 121 VFYYGDINSPIHFRGSWEMSRLNLHVPAGMPDHEYLDSDFDDFFAPSPRSILCCRVVAVI 179

[Close]

BLAST of Spg030832 vs. NCBI nr
Match: XP_022143288.1 (uncharacterized protein LOC111013195 isoform X3 [Momordica charantia] >XP_022143289.1 uncharacterized protein LOC111013195 isoform X3 [Momordica charantia] >XP_022143290.1 uncharacterized protein LOC111013195 isoform X3 [Momordica charantia])

HSP 1 Score: 319.7 bits (818), Expect = 1.8e-83
Identity = 147/178 (82.58%), Postives = 161/178 (90.45%), Query Frame = 0


Query: 1   MGDHFVLLVNRLLTESNLEATIERKNRICQPMASTNADNMISSVDIDVESISPSSKLVEC 60
           MGDHFVLLV+RLLTESN+EATIE KNRI QPMASTN DN+ISS++IDV+S+ PSSK VEC
Sbjct: 1   MGDHFVLLVDRLLTESNVEATIESKNRIHQPMASTNVDNVISSLNIDVDSMLPSSKSVEC 60

Query: 61  RICYDEDDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDFKPGYAAPPPVF 120
           RIC+DED ESNMETPCSCCGSLKYAHRKCVQ WC+EKGNT CEICHQ FKPG+ APPPVF
Sbjct: 61  RICHDEDVESNMETPCSCCGSLKYAHRKCVQRWCNEKGNTMCEICHQQFKPGFTAPPPVF 120

Query: 121 YYGGINSPIHFRGSWEMSRLKLHVPAGMPDHEYLNSDFDDFLTPSPRSTLCCCIVAVI 179
           YYGGI SPIHFRG+WEMSRL LHV AGMPDH+Y++SD DDFL PSPRS LCC IVAVI
Sbjct: 121 YYGGIGSPIHFRGNWEMSRLNLHVSAGMPDHDYVDSDIDDFLAPSPRSVLCCRIVAVI 178

[Close]

BLAST of Spg030832 vs. NCBI nr
Match: XP_022143286.1 (uncharacterized protein LOC111013195 isoform X1 [Momordica charantia])

HSP 1 Score: 319.7 bits (818), Expect = 1.8e-83
Identity = 147/178 (82.58%), Postives = 161/178 (90.45%), Query Frame = 0


Query: 1   MGDHFVLLVNRLLTESNLEATIERKNRICQPMASTNADNMISSVDIDVESISPSSKLVEC 60
           MGDHFVLLV+RLLTESN+EATIE KNRI QPMASTN DN+ISS++IDV+S+ PSSK VEC
Sbjct: 9   MGDHFVLLVDRLLTESNVEATIESKNRIHQPMASTNVDNVISSLNIDVDSMLPSSKSVEC 68

Query: 61  RICYDEDDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDFKPGYAAPPPVF 120
           RIC+DED ESNMETPCSCCGSLKYAHRKCVQ WC+EKGNT CEICHQ FKPG+ APPPVF
Sbjct: 69  RICHDEDVESNMETPCSCCGSLKYAHRKCVQRWCNEKGNTMCEICHQQFKPGFTAPPPVF 128

Query: 121 YYGGINSPIHFRGSWEMSRLKLHVPAGMPDHEYLNSDFDDFLTPSPRSTLCCCIVAVI 179
           YYGGI SPIHFRG+WEMSRL LHV AGMPDH+Y++SD DDFL PSPRS LCC IVAVI
Sbjct: 129 YYGGIGSPIHFRGNWEMSRLNLHVSAGMPDHDYVDSDIDDFLAPSPRSVLCCRIVAVI 186

[Close]

BLAST of Spg030832 vs. NCBI nr
Match: XP_022143287.1 (uncharacterized protein LOC111013195 isoform X2 [Momordica charantia])

HSP 1 Score: 318.9 bits (816), Expect = 3.0e-83
Identity = 147/179 (82.12%), Postives = 161/179 (89.94%), Query Frame = 0


Query: 1   MGDHFVLLVNRLLTESNLEATIERKNRICQPMASTNADNMISSVDIDVESISPSSKLVEC 60
           MGDHFVLLV+RLLTESN+EATIE KNRI QPMASTN DN+ISS++IDV+S+ PSSK VEC
Sbjct: 9   MGDHFVLLVDRLLTESNVEATIESKNRIHQPMASTNVDNVISSLNIDVDSMLPSSKSVEC 68

Query: 61  RICYDEDDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDFKPGYAAPPPVF 120
           RIC+DED ESNMETPCSCCGSLKYAHRKCVQ WC+EKGNT CEICHQ FKPG+ APPPVF
Sbjct: 69  RICHDEDVESNMETPCSCCGSLKYAHRKCVQRWCNEKGNTMCEICHQQFKPGFTAPPPVF 128

Query: 121 YYGGINSPIHFRGSWEMSRLKLHVPAGMPDHEYLNSDFDDFLTPSPRSTLCCCIVAVIV 180
           YYGGI SPIHFRG+WEMSRL LHV AGMPDH+Y++SD DDFL PSPRS LCC IVA IV
Sbjct: 129 YYGGIGSPIHFRGNWEMSRLNLHVSAGMPDHDYVDSDIDDFLAPSPRSVLCCRIVAFIV 187

[Close]

BLAST of Spg030832 vs. ExPASy Swiss-Prot
Match: Q5T0T0 (E3 ubiquitin-protein ligase MARCHF8 OS=Homo sapiens OX=9606 GN=MARCHF8 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 6.7e-09
Identity = 29/61 (47.54%), Postives = 39/61 (63.93%), Query Frame = 0


Query: 50  SISPSSKLVECRICYDE-DDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQD 109
           SI+PSS+ + CRIC+ E DDES + TPC C GSL + H+ C+Q W        CE+C  +
Sbjct: 71  SITPSSQDI-CRICHCEGDDESPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKYE 130

[Close]

BLAST of Spg030832 vs. ExPASy Swiss-Prot
Match: Q28IK8 (E3 ubiquitin-protein ligase MARCHF8 OS=Xenopus tropicalis OX=8364 GN=marchf8 PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.5e-08
Identity = 34/100 (34.00%), Postives = 53/100 (53.00%), Query Frame = 0


Query: 11  RLLTESNLEATIERKNRICQPMASTNADNMISSVDIDVESISPSSKLVECRICYDE-DDE 70
           +L  E  L  ++ R + I +  + T+     +       S++PSS+ + CRIC+ E DDE
Sbjct: 8   KLQNEKTLGHSVSRSSNISKAGSPTSVS---APSSFPRTSVTPSSQDI-CRICHCEGDDE 67

Query: 71  SNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDF 110
           S + TPC C GSL + H+ C+Q W        CE+C  +F
Sbjct: 68  SPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKFEF 103

[Close]

BLAST of Spg030832 vs. ExPASy Swiss-Prot
Match: Q0VD59 (E3 ubiquitin-protein ligase MARCHF8 OS=Bos taurus OX=9913 GN=MARCHF8 PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.9e-08
Identity = 34/96 (35.42%), Postives = 52/96 (54.17%), Query Frame = 0


Query: 15  ESNLEATIERKNRICQPMASTNADNMISSVDIDVESISPSSKLVECRICYDE-DDESNME 74
           E  L  ++   + I +   S+ A   +SS      S++PS++ + CRIC+ E DDES + 
Sbjct: 36  EKTLGHSMSHSSNISKAGGSSVASAPVSS--FPRTSVTPSNQDI-CRICHCEGDDESPLI 95

Query: 75  TPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDF 110
           TPC C GSL + H+ C+Q W        CE+C  +F
Sbjct: 96  TPCRCTGSLHFVHQTCLQQWIKSSDTRCCELCKYEF 128

[Close]

BLAST of Spg030832 vs. ExPASy Swiss-Prot
Match: Q9DBD2 (E3 ubiquitin-protein ligase MARCHF8 OS=Mus musculus OX=10090 GN=Marchf8 PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.9e-08
Identity = 27/61 (44.26%), Postives = 39/61 (63.93%), Query Frame = 0


Query: 50  SISPSSKLVECRICYDE-DDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQD 109
           S++PS++ + CRIC+ E DDES + TPC C GSL + H+ C+Q W        CE+C  +
Sbjct: 67  SVTPSNQDI-CRICHCEGDDESPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKYE 126

[Close]

BLAST of Spg030832 vs. ExPASy Swiss-Prot
Match: Q5XH39 (E3 ubiquitin-protein ligase MARCHF8 OS=Xenopus laevis OX=8355 GN=marchf8 PE=2 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 2.5e-08
Identity = 34/100 (34.00%), Postives = 53/100 (53.00%), Query Frame = 0


Query: 11  RLLTESNLEATIERKNRICQPMASTNADNMISSVDIDVESISPSSKLVECRICYDE-DDE 70
           +L  E  L  ++ R + I +  + T+     +       S++PSS+ + CRIC+ E DDE
Sbjct: 8   KLQNEKTLGHSVSRSSNISKAGSPTSVS---APSRFPRTSVTPSSQDI-CRICHCEGDDE 67

Query: 71  SNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDF 110
           S + TPC C GSL + H+ C+Q W        CE+C  +F
Sbjct: 68  SPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKFEF 103

[Close]

BLAST of Spg030832 vs. ExPASy TrEMBL
Match: A0A0A0LBM8 (RING-CH-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G186720 PE=4 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 2.9e-84
Identity = 150/180 (83.33%), Postives = 163/180 (90.56%), Query Frame = 0


Query: 1   MGDHFVLLVNRLLTESNLEATIERKNRICQPMAST--NADNMISSVDIDVESISPSSKLV 60
           MGDHF LLV++LLTESNLEATIERKNRIC PMAST  NADNMISS +IDVES+SPSS +V
Sbjct: 1   MGDHFGLLVDQLLTESNLEATIERKNRICHPMASTNANADNMISSSNIDVESVSPSS-IV 60

Query: 61  ECRICYDEDDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDFKPGYAAPPP 120
           +CRIC+DEDD S METPCSCCGSLKYAHRKC+Q WC+EKG+T CEICHQDFKPGY +PPP
Sbjct: 61  QCRICHDEDDGSKMETPCSCCGSLKYAHRKCIQRWCNEKGDTICEICHQDFKPGYTSPPP 120

Query: 121 VFYYGGINSPIHFRGSWEMSRLKLHVPAGMPDHEYLNSDFDDFLTPSPRSTLCCCIVAVI 179
           VFYYG INSPIHFRGSWEMSRL LHVPAGMPDHEYL+SDFDDF  PSPRS LCC +VAVI
Sbjct: 121 VFYYGDINSPIHFRGSWEMSRLNLHVPAGMPDHEYLDSDFDDFFAPSPRSILCCRVVAVI 179

[Close]

BLAST of Spg030832 vs. ExPASy TrEMBL
Match: A0A6J1CNE6 (uncharacterized protein LOC111013195 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111013195 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 8.6e-84
Identity = 147/178 (82.58%), Postives = 161/178 (90.45%), Query Frame = 0


Query: 1   MGDHFVLLVNRLLTESNLEATIERKNRICQPMASTNADNMISSVDIDVESISPSSKLVEC 60
           MGDHFVLLV+RLLTESN+EATIE KNRI QPMASTN DN+ISS++IDV+S+ PSSK VEC
Sbjct: 1   MGDHFVLLVDRLLTESNVEATIESKNRIHQPMASTNVDNVISSLNIDVDSMLPSSKSVEC 60

Query: 61  RICYDEDDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDFKPGYAAPPPVF 120
           RIC+DED ESNMETPCSCCGSLKYAHRKCVQ WC+EKGNT CEICHQ FKPG+ APPPVF
Sbjct: 61  RICHDEDVESNMETPCSCCGSLKYAHRKCVQRWCNEKGNTMCEICHQQFKPGFTAPPPVF 120

Query: 121 YYGGINSPIHFRGSWEMSRLKLHVPAGMPDHEYLNSDFDDFLTPSPRSTLCCCIVAVI 179
           YYGGI SPIHFRG+WEMSRL LHV AGMPDH+Y++SD DDFL PSPRS LCC IVAVI
Sbjct: 121 YYGGIGSPIHFRGNWEMSRLNLHVSAGMPDHDYVDSDIDDFLAPSPRSVLCCRIVAVI 178

[Close]

BLAST of Spg030832 vs. ExPASy TrEMBL
Match: A0A6J1CPU3 (uncharacterized protein LOC111013195 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013195 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 8.6e-84
Identity = 147/178 (82.58%), Postives = 161/178 (90.45%), Query Frame = 0


Query: 1   MGDHFVLLVNRLLTESNLEATIERKNRICQPMASTNADNMISSVDIDVESISPSSKLVEC 60
           MGDHFVLLV+RLLTESN+EATIE KNRI QPMASTN DN+ISS++IDV+S+ PSSK VEC
Sbjct: 9   MGDHFVLLVDRLLTESNVEATIESKNRIHQPMASTNVDNVISSLNIDVDSMLPSSKSVEC 68

Query: 61  RICYDEDDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDFKPGYAAPPPVF 120
           RIC+DED ESNMETPCSCCGSLKYAHRKCVQ WC+EKGNT CEICHQ FKPG+ APPPVF
Sbjct: 69  RICHDEDVESNMETPCSCCGSLKYAHRKCVQRWCNEKGNTMCEICHQQFKPGFTAPPPVF 128

Query: 121 YYGGINSPIHFRGSWEMSRLKLHVPAGMPDHEYLNSDFDDFLTPSPRSTLCCCIVAVI 179
           YYGGI SPIHFRG+WEMSRL LHV AGMPDH+Y++SD DDFL PSPRS LCC IVAVI
Sbjct: 129 YYGGIGSPIHFRGNWEMSRLNLHVSAGMPDHDYVDSDIDDFLAPSPRSVLCCRIVAVI 186

[Close]

BLAST of Spg030832 vs. ExPASy TrEMBL
Match: A0A6J1CQD4 (uncharacterized protein LOC111013195 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013195 PE=4 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.5e-83
Identity = 147/179 (82.12%), Postives = 161/179 (89.94%), Query Frame = 0


Query: 1   MGDHFVLLVNRLLTESNLEATIERKNRICQPMASTNADNMISSVDIDVESISPSSKLVEC 60
           MGDHFVLLV+RLLTESN+EATIE KNRI QPMASTN DN+ISS++IDV+S+ PSSK VEC
Sbjct: 9   MGDHFVLLVDRLLTESNVEATIESKNRIHQPMASTNVDNVISSLNIDVDSMLPSSKSVEC 68

Query: 61  RICYDEDDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDFKPGYAAPPPVF 120
           RIC+DED ESNMETPCSCCGSLKYAHRKCVQ WC+EKGNT CEICHQ FKPG+ APPPVF
Sbjct: 69  RICHDEDVESNMETPCSCCGSLKYAHRKCVQRWCNEKGNTMCEICHQQFKPGFTAPPPVF 128

Query: 121 YYGGINSPIHFRGSWEMSRLKLHVPAGMPDHEYLNSDFDDFLTPSPRSTLCCCIVAVIV 180
           YYGGI SPIHFRG+WEMSRL LHV AGMPDH+Y++SD DDFL PSPRS LCC IVA IV
Sbjct: 129 YYGGIGSPIHFRGNWEMSRLNLHVSAGMPDHDYVDSDIDDFLAPSPRSVLCCRIVAFIV 187

[Close]

BLAST of Spg030832 vs. ExPASy TrEMBL
Match: A0A6J1FB67 (uncharacterized protein LOC111442434 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442434 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 6.1e-82
Identity = 143/177 (80.79%), Postives = 160/177 (90.40%), Query Frame = 0


Query: 1   MGDHFVLLVNRLLTESNLEATIERKNRICQPMASTNADNMISSVDIDVESISPSSKLVEC 60
           MG+HFVLLV++LLTESNLE+TIERKNR C+PMAST+ADNMISS++IDVE +SPSSKLV+C
Sbjct: 1   MGEHFVLLVDQLLTESNLESTIERKNRNCRPMASTSADNMISSLNIDVEFMSPSSKLVQC 60

Query: 61  RICYDEDDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDFKPGYAAPPPVF 120
           RIC+DEDDES METPCSCCGSLKYAHR CVQ WC+EKGNT CEICHQDF+PGY AP PV 
Sbjct: 61  RICHDEDDESKMETPCSCCGSLKYAHRMCVQRWCNEKGNTICEICHQDFEPGYTAPLPVL 120

Query: 121 YYGGINSPIHFRGSWEMSRLKLHVPAGMPDHEYLNSDFDDFLTPSPRSTLCCCIVAV 178
           YYG I SP+HFR SWEMSRL LHVPAGMPDHEY +SDFD+F TPSPRS LCC +VAV
Sbjct: 121 YYGDITSPVHFRESWEMSRLNLHVPAGMPDHEYPDSDFDEFFTPSPRSILCCRMVAV 177

[Close]

BLAST of Spg030832 vs. TAIR 10
Match: AT2G01275.1 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 170.6 bits (431), Expect = 1.2e-42
Identity = 86/181 (47.51%), Postives = 119/181 (65.75%), Query Frame = 0


Query: 1   MGDHFVLLVNRLLTESNLEATIERKNRICQPMASTNADNMISSVDIDVESISPSSKLVEC 60
           MGDHFVLLV+RL+TES +EA I+ +N++ Q         ++    +++      S + EC
Sbjct: 1   MGDHFVLLVDRLITESTIEAAIQSRNQMLQANIPVEECTILDDKTLEMLRNGDLS-MAEC 60

Query: 61  RICYDEDDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDFKPGYAAPPPVF 120
           RIC+DED +SNMETPCSC GS+KYAHR+CVQ WC+EKG+TTCEICHQ+FKP Y APPP+ 
Sbjct: 61  RICHDEDLDSNMETPCSCSGSVKYAHRRCVQRWCNEKGDTTCEICHQEFKPDYTAPPPLL 120

Query: 121 YYGGINSPIHFRGSWEMSRLKLHVPAGMPDHEYLNSDFDDFLTP-SPRSTLCCCIVAVIV 180
             G +  P+HFRG+W +S+ +      +P     +S F D   P S  ++  CC   V++
Sbjct: 121 ELGHV--PLHFRGNWGISQREHRFITVVP----ADSTFIDQPYPLSSTTSFICCRSLVLI 174

[Close]

BLAST of Spg030832 vs. TAIR 10
Match: AT2G01275.2 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 170.6 bits (431), Expect = 1.2e-42
Identity = 86/181 (47.51%), Postives = 119/181 (65.75%), Query Frame = 0


Query: 1   MGDHFVLLVNRLLTESNLEATIERKNRICQPMASTNADNMISSVDIDVESISPSSKLVEC 60
           MGDHFVLLV+RL+TES +EA I+ +N++ Q         ++    +++      S + EC
Sbjct: 1   MGDHFVLLVDRLITESTIEAAIQSRNQMLQANIPVEECTILDDKTLEMLRNGDLS-MAEC 60

Query: 61  RICYDEDDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDFKPGYAAPPPVF 120
           RIC+DED +SNMETPCSC GS+KYAHR+CVQ WC+EKG+TTCEICHQ+FKP Y APPP+ 
Sbjct: 61  RICHDEDLDSNMETPCSCSGSVKYAHRRCVQRWCNEKGDTTCEICHQEFKPDYTAPPPLL 120

Query: 121 YYGGINSPIHFRGSWEMSRLKLHVPAGMPDHEYLNSDFDDFLTP-SPRSTLCCCIVAVIV 180
             G +  P+HFRG+W +S+ +      +P     +S F D   P S  ++  CC   V++
Sbjct: 121 ELGHV--PLHFRGNWGISQREHRFITVVP----ADSTFIDQPYPLSSTTSFICCRSLVLI 174

[Close]

BLAST of Spg030832 vs. TAIR 10
Match: AT5G38070.1 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 150.6 bits (379), Expect = 1.3e-36
Identity = 76/178 (42.70%), Postives = 109/178 (61.24%), Query Frame = 0


Query: 1   MGDHFVLLVNRLLTESNLEATIERKNRICQPMASTNADNMISSVDIDVESISPSSKLVEC 60
           MGDH VL+V+RL ++S L       NR      S N D +  S+    +     SK V+C
Sbjct: 1   MGDHLVLVVDRLSSDSGLGTV----NRADPKADSVNEDGVSESISAGADLC--ESKFVQC 60

Query: 61  RICYDEDDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDFKPGYAAPPPVF 120
           RIC+DED+++NM+TPCSC G+LK+AH  CVQ WC+EKG+T CEIC Q +KPGY AP  +F
Sbjct: 61  RICHDEDEDTNMDTPCSCSGTLKFAHHNCVQRWCNEKGDTVCEICRQQYKPGYTAPRQLF 120

Query: 121 YYGGINSPIHFRGSWEMSRLKLHVPAGMPDHEYLNSDFDDFLTPSPRSTLCCCIVAVI 179
           +Y GI+  ++F   W +  L L  P  +   +  + D D +   SP S +CC ++A++
Sbjct: 121 HYTGIS--MNFGSDWGIEGLDLRNPYFLTWGD-ADDDHDLYSFHSPTSLICCRLIALL 169

[Close]

BLAST of Spg030832 vs. TAIR 10
Match: AT1G14260.1 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 121.7 bits (304), Expect = 6.6e-28
Identity = 68/165 (41.21%), Postives = 87/165 (52.73%), Query Frame = 0


Query: 4   HFVLLVNRLLTESNLEATIERKNRICQPMASTNADNMISSVDIDVESISPSSKLV---EC 63
           H V+ VN L+     EA    +         + ADN I   D D         L+   EC
Sbjct: 5   HTVVYVNGLVRPVLAEAEYSMR-------TESPADNAIDIYDGDTTENEEEDSLISSAEC 64

Query: 64  RICYDEDDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDFKPGYAAPPPVF 123
           RIC DE D  N+E+PC+C GSLKYAHRKCVQ WC+EKGNT CEICHQ ++ GY +PPP  
Sbjct: 65  RICQDECDIKNLESPCACNGSLKYAHRKCVQRWCNEKGNTICEICHQPYQAGYTSPPPP- 124

Query: 124 YYGGINSPIHFRGSWEMSRLKLHVP----AGMPDHEYLNSDFDDF 162
                 + I   G W +S L L  P        + + L S++DD+
Sbjct: 125 -PQSEETTIDIGGGWRISGLDLDDPRLLAIAEAERQILESEYDDY 160

[Close]

BLAST of Spg030832 vs. TAIR 10
Match: AT1G14260.2 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 121.7 bits (304), Expect = 6.6e-28
Identity = 68/165 (41.21%), Postives = 87/165 (52.73%), Query Frame = 0


Query: 4   HFVLLVNRLLTESNLEATIERKNRICQPMASTNADNMISSVDIDVESISPSSKLV---EC 63
           H V+ VN L+     EA    +         + ADN I   D D         L+   EC
Sbjct: 5   HTVVYVNGLVRPVLAEAEYSMR-------TESPADNAIDIYDGDTTENEEEDSLISSAEC 64

Query: 64  RICYDEDDESNMETPCSCCGSLKYAHRKCVQWWCDEKGNTTCEICHQDFKPGYAAPPPVF 123
           RIC DE D  N+E+PC+C GSLKYAHRKCVQ WC+EKGNT CEICHQ ++ GY +PPP  
Sbjct: 65  RICQDECDIKNLESPCACNGSLKYAHRKCVQRWCNEKGNTICEICHQPYQAGYTSPPPP- 124

Query: 124 YYGGINSPIHFRGSWEMSRLKLHVP----AGMPDHEYLNSDFDDF 162
                 + I   G W +S L L  P        + + L S++DD+
Sbjct: 125 -PQSEETTIDIGGGWRISGLDLDDPRLLAIAEAERQILESEYDDY 160

The following BLAST results are available for this feature:

BLAST of Spg030832 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031738305.1	6.1e-84	83.33	E3 ubiquitin-protein ligase MARCH2 isoform X1 [Cucumis sativus]	[more]
XP_011651201.1	6.1e-84	83.33	E3 ubiquitin-protein ligase MARCH2 isoform X2 [Cucumis sativus] >XP_011651203.1 ...	[more]
XP_022143288.1	1.8e-83	82.58	uncharacterized protein LOC111013195 isoform X3 [Momordica charantia] >XP_022143...	[more]
XP_022143286.1	1.8e-83	82.58	uncharacterized protein LOC111013195 isoform X1 [Momordica charantia]	[more]
XP_022143287.1	3.0e-83	82.12	uncharacterized protein LOC111013195 isoform X2 [Momordica charantia]	[more]

BLAST of Spg030832 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5T0T0	6.7e-09	47.54	E3 ubiquitin-protein ligase MARCHF8 OS=Homo sapiens OX=9606 GN=MARCHF8 PE=1 SV=1	[more]
Q28IK8	1.5e-08	34.00	E3 ubiquitin-protein ligase MARCHF8 OS=Xenopus tropicalis OX=8364 GN=marchf8 PE=...	[more]
Q0VD59	1.9e-08	35.42	E3 ubiquitin-protein ligase MARCHF8 OS=Bos taurus OX=9913 GN=MARCHF8 PE=2 SV=1	[more]
Q9DBD2	1.9e-08	44.26	E3 ubiquitin-protein ligase MARCHF8 OS=Mus musculus OX=10090 GN=Marchf8 PE=2 SV=...	[more]
Q5XH39	2.5e-08	34.00	E3 ubiquitin-protein ligase MARCHF8 OS=Xenopus laevis OX=8355 GN=marchf8 PE=2 SV...	[more]

BLAST of Spg030832 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LBM8	2.9e-84	83.33	RING-CH-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G18672...	[more]
A0A6J1CNE6	8.6e-84	82.58	uncharacterized protein LOC111013195 isoform X3 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1CPU3	8.6e-84	82.58	uncharacterized protein LOC111013195 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1CQD4	1.5e-83	82.12	uncharacterized protein LOC111013195 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1FB67	6.1e-82	80.79	uncharacterized protein LOC111442434 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of Spg030832 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G01275.1	1.2e-42	47.51	RING/FYVE/PHD zinc finger superfamily protein	[more]
AT2G01275.2	1.2e-42	47.51	RING/FYVE/PHD zinc finger superfamily protein	[more]
AT5G38070.1	1.3e-36	42.70	RING/FYVE/PHD zinc finger superfamily protein	[more]
AT1G14260.1	6.6e-28	41.21	RING/FYVE/PHD zinc finger superfamily protein	[more]
AT1G14260.2	6.6e-28	41.21	RING/FYVE/PHD zinc finger superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 59..106 e-value: 1.5E-18 score: 77.6
IPR011016	Zinc finger, RING-CH-type	PFAM	PF12906	RINGv	coord: 60..105 e-value: 7.3E-12 score: 45.2
IPR011016	Zinc finger, RING-CH-type	PROSITE	PS51292	ZF_RING_CH	coord: 52..112 score: 20.747803
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 54..111 e-value: 7.6E-18 score: 66.2
IPR022143	Protein of unknown function DUF3675	PFAM	PF12428	DUF3675	coord: 111..179 e-value: 7.9E-9 score: 36.1
None	No IPR available	PANTHER	PTHR23012:SF93	OS01G0121200 PROTEIN	coord: 1..179
None	No IPR available	CDD	cd16495	RING_CH-C4HC3_MARCH	coord: 60..106 e-value: 2.61792E-15 score: 65.0412
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 58..110
IPR033275	E3 ubiquitin-protein ligase MARCH-like	PANTHER	PTHR23012	RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAINING	coord: 1..179

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg030832.1	Spg030832.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004842	ubiquitin-protein transferase activity
molecular_function	GO:0008270	zinc ion binding