Spg030774 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg030774
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Threonyl-tRNA synthetase
Location	scaffold11: 27262070 .. 27269941 (-)
RNA-Seq Expression	Spg030774
Synteny	Spg030774

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCGTTATCAAAAGAGACTTCTTTTGGGCTGGTGGAGATAACAGAGTTGGTAGCCACCTGGTGAATTGGGTTGTTGTTTCTCTTCCTCTTTGTCAAGGAGGTTTGGGGATTGGAGGCTCGAAGCATCGGAATGCAGCCCTTCTTACGAAGAACGAGGAAAATGCTTTTTGGAGGCAGGTGGTGGCTAGTATTTACGGGGAAGATCCTCATGGGTGGTTGGTTTCCCGTTTCACTAGAGCTCCAAGAATAGACCTTGGTTGGACATCGCTAAGATGGTGCCTCTATATGAGAAGTTCATCAGGTTTAAAACTGGGAATGGGCAACACATTAGTTTTTGGAAGAACAAATGGCTGGGCGAGACCCTGATGATTTTCCTGAAATTTACAGTATTTCCTTGAAGACAAAGGCCACAGTGGCTGATTGTTGGGATGAAACTTAGGTGACTTGGAACTTGGGTATTAGAAGAGGGGTTTTTGAGAGAGAATTGCAGGCGTGGATCAGGATGACAGAAAATCTGGAAGGTTTACGCTTGGGGCAAGAAGCGGATATAATTATCTGCTCATTGGACTCTACTGAAGTCTTCTCCACCAAATCTTTGTTTCTCAGGTTGGGGTCTCTAGATCCTTATTAAGATGTCCTCTCCCTAGGTATGGGAGTTCAAAGTCCCAAAAAAAGGTTAAAATTTTTCTTTGGGTTGTGGTCCGTAGATGTCTTCATACTCACGAGAGGTTGCAAAAGGGGTGCCCAAACTTTGCGCTCTGTCCCTCTGTTTGTTCCCTCTGTTTGCTTAATGAAGAATCTTTGGATCATATTTTTTTGCACTGCCGTTTTGCCAGACAATGTTGGGACAGATTCATGAGCATGTTTAATATTCAAATTTGTCTCCCAAGGAAGGTGGATGATTGGTTGCTGGAATCGTTGAGTGGCTGGAAGCACAAGAGAAAAGCTAGGAATCTGTGGATGTTTTCCTCGAGATACCTGTTATGAAGTTTGTGGCTGGAATGAAATAGAAGGATCTTTGATGATAAATCTTCTTCTTTTGCTATTTTTTTGGGATAGTGTGCATATTAATGCTTCTTGGTGCTGCTATAGCTTTGCCAAATTCTTTTGTAATTATCCTTTCTATTTGTTCCTTTAAGATTGGAGGGCTTTTTGTATGTAGTTCCTCGGGTAGGGCAGCCCTCTTCCCCTAGTCCTTGGGTTGATTCTCCTCGTCTTGGTTTAATCTATCTCTGATGGTTTCTTATTAAAAAAAAAAAAAAAAAGAGAGAGAATTGAAGTTTCAAGGAAATGGTGCTTGGAGGCTTCTTGTGTTTATTCAGTCTATTCTCTCTCTAGTAAGTTGGTGGTTTCTTCTCCTGTGCGAAAAGATTTTTTTTTTCTCAGCTTTATGAAAAACAAAATCTCCGAAAAGAATCAACATTCGTTTCTTAATAGTTGTTTGAATACAGCTGACGTGCTTCAAAGGAAGATTCCTACTTCATTTCTTTTGCCTTCTATATGTTGTCTTTGCTATGCAGACGGGGATTCTCTTAATCATCTATTCTTTGGATGTTCTTTCTCAATGGCTTGTTGGTTCAAACTATTCAGTATCTTCAATTTGCAGTGGGTGTTTTCGTATTCTGCCAAAGATGATGTTCTTCAGCTTTCATTGGACTTATCTTTTACCTTTGAGACCTCATTTACTATGGATCAACACAGTCAAAGCTTTGATTTCAAAATTATGGTTAGGGAGAAATAAGAGGAATTTTTAAGAGAAATGTTGTTCTAAGGATTTTTTAAGAGAAATGTTGTTCTTGGATAGATCGGTTTTATTTATCTCATCTTAAAGCTTCCCCGTAGTGTTCTCTTTCCAACATTTTTAATGGCTTCTCATTGCAAGATATTCATTTGAATTGGAATGGTTTTATATGACACCCACAATTTTGTTTTTTCATTTTTTCTTCACTCCTTTTGGAGTTTGCATCTTTGGAACATTACTCTCTTTTCATTTTCCAAAAAATACTTACGTTTCTTGTTTTTTTTAAAAAATACTTGCGTTTTAGAAATAAATATATAAATTTTTGGGGAAATAAATGGTGAGCTCCATATGTTCTCCTTACCAAAAAAGAGGAAACCAAACCAGATAGACACCACTGTACTCTATCCTCCTAGTATATTCTCCATAAAGCGGTTTCTAACCTTGGTTCTTCATTAACTATACACCCCTGCTTGCTTTTTTCACGTTCTCTTCTATTCTTTCCTTTCTTCGATAAATCCATTTAACCTTGGGCCTATGCACTCCTAGTCCTTGCGTGTTTCTTAATGATTACTATGTTAATGCCCAAGAATCTTTATATCATAAATACTCTTCTTCCTATCTAATTCTCAACTTGAATGCTGTGTTTTTTCTTTTTGCTAGTAGAAGCTTTCGTTTGAGTGACAGATTCATGTTTAACATCTCTCTGTTTTTAAATATTATTATTTATTATAATGTTATAGAGGCCAGAGAAATACCTTGGAGATTTGGAAACATGGGAGAAAGCTGAAGCTGCACTTACAGAAGCACTAAATGAATTCGGGAAGCCCTGGCAGGTACTTTTAATGTGGTTGAACCTGCATTAAGTTTTCCTTTGATGCACTTAATTGCTAGTATAGTGACATGAACCTCAGGATTTTGAATTCACTTTATAATTGGTTTTAAAATTTTCCTAGTTCTAGTTAAATTTCTTTGCACTCTTTATTGAATTTGGCTAAATTGAAGTTCAATCTGTTTGAGACAGCCACCCTATGAATAAGTTTTAGCTCGATCAATAGAATCTCCTTTCTCTCTCTGCTTATTCCATCATTAAATATTTGCAAATTTGTGTCACTACTTGTTGATCTTGCTTATCCATCAGTTTAGTTTTACATGACTCTACTCATATTGATGTTTACTCTATGTCTTTTTGTCACTCTACATTCTCCTATTGTGTTTTATTTGCTGAATATGCTTTCTTAAAGCAAAACTTTAACCTTGCAATTTCACAAGGTTTTTCCAAGCAAGTTTCCAACCATAACAATTTGGTAGCAATGGGAAAGGAAAAATTGAAGTCCAAAGAGTTACCTAGTTCAAAAAAAGAGCTACATATATGAGTTAGTTTGTTTGACAAGAATGGAGTCTCTCTTTCTTGCTTCTGTTTTACTCTGTCTATTGTTATGTATATATTTGCTTCTTTTTGGTTCTGTCTATTGTTGCTATCTCTTAGAATTTTTGAAGATTTGTCTAGTGAGTAAAGTGAAAGATATTATTTTGTGGAAACAAGGGCAAAGAGTGGCATTAGACATGCAGAAACCTTTGATTTCGTTAGTGTTATATTTTGTGAGGATCTATAGGAGTATAAAAAAATTTCCAGTTTGGTTTGGTTTTATTGTTAAAAATCGAAAAAAGAAGGAAAAAAAAAATCTGAATTGAGGCTATGTACGTATTAGGGAAAAACCCTAGAATTTCAATTGACTTTCATTCTCTCTTATCTTCATACTTGCGTGTGGTGGCAGAGCAGTAGACCCTTCACACAATTCCATTAGCTCCATTGATCAACCCCGCCCTTAGACCTCACAACTCATCCTATAAACAACATTTGCTTCTTTTAAAAGCTTCAACACTAAGGTCATTATCTTCCGATTATGAAAAGATATATTTAAATATTTTTGTTTGTCAAATGAAATCTTGTTCAGAAACTGTCTTAGCCCTTCTGTATGAATCCCATTCCACTAAATCAGATATCCCAGTCAAGTTTGATACGTGGCTCAACCCAAGTTAGTTATGAGAATTGTTCCAAAGTGTTAACAACTTGAAGAATTGGTCTGTATTATCTACTCTTCTTCTTTGTTGGTAAATATGTCCATAAATTTACAAAATTTGGTATAAGACTTAGCCCAAGTATGAGTGCAAAGGGTCATTGCTCCAAAGTGTTAACGACTTAATTGCAGATAGAAGATTGGTTTCTCCGATTAGTTGGTAACTTCTCCCAATTTGTTATTTATGGTGATTGCTCCTTTGCTCACACCTCTTTGAAATGTGGACCCTCAAATTTTTCTAATCTTCTTTTGGTATCACAAAGTGTTTGTCTTCTTCCAAATACAAGTGACTCTCTTGCATTTATATCTTCTTCTAAATCTGCTTTTGATTTTCTTATTTCACATGTCATTTCTCTTGTTCTCCACCCTTCTTTCAATGAAATATGGAGGCTTAAATCTGAAGGAAGATCAAGTTTTTTGCTTGGGGTTCTTTGACGAGGTTAAACATCATGGACTCTGTCCAAAATAGCAATCGACTCTTTAGGTCTTATTTTACTTACATGACTGACTTAGCATTTGTTTTTTTAGGTATGCCCTTGTATTCAAATTTTTCACGATGAAAGTTGGTTTTTCATTAATAGGAATCATGAAACCTGTCCAAAGATCTTCCATGGTTTTGGGTCCTTAGTGATGTGTTAAGTAGATTAGCCACAGTAGATTTATATCATTTACATTGAAATTGTCATGTTGTGAGACTCTGGACCAATTTTATGGACATTTGGAATCAGTTTGGCTATTTCCAGATGTTGTAAGGATACCTTGGAAGAGGTGGTTGTTTTTCCTTCTTTCTGGAGAAGGGGTCAGATTCTTTGGCAAGTGGCTTTCTTTGCTATGTTGTAGAATATTTAGCATGAGAGAATGTGAGAGTCTTAAGAGATACTAAGAAGACTTTAGAGGATGTCTAGACTCTAGAATGTAAAGCATGCCAATGTTTCTCTATGGATAGCTTGTCCATAAAGTTCGTGACTCTGTCTTATATTTTCTTTTTAACTCTTAACAATTTGAGCAATCTCTTGTAATTGACTTTTGTTTTGTTTGCTCAATCCATTGGGCCATTCTGGTCTTATAAAAAATAAATAAATGTTAGTGAAGGAAAGTTAGCTTAATTATGTTATCCCAGTTTTTTGTTCACTCTCTTGCTATATTAAAGATATCTTCCTACATTTTTTTCCTTGATTGGCTTTGAGTCATGATGCTCTATATCATAAGAGAACTGTGTGTGGTTATGTGAATTTTTCTTTTCTTGTGTTTCAAGCTCATTCCAAGAATTTCTATTTTCATTTTGGATTTTTGTTCTAGATCATTATTCTTTCATCTTCTTTGATGGATTTATATCATATCTAAGGTGGAGATATAAGATATGAATTTGTATCATATCTTACAATTCTCTCTTTGGACTCCATTTTAGCACTCCATGCTTATAAACTATTGAGATTTGTTTCTCCTTGACCAATGAGTCGTCTGATATAGATCAATGAAGGAGATGGTGCATTTTATGGACCGAAGATAGATATTAGTGTCTCCGATGCATTGAAAAGAAAATTTCAATGTGCAACCCTTCAGGTATTATATACTTATTTATTTTTATAGTTTCTTGCTCACATTTGTTGATTGGGTTTTAATGCAAACAAAATTATTTTGCAACATGGGCGTATTTATTTGCAGTTGGACTTCCAGCTGCCTTCTCGCTTTGATTTGTATTACTCAGCAGAGGATGAGGCCAAAAGGGAACGACCTGTTATGATTCATAGAGCAATCTTGGGGTCTGTAGAGAGAATGTTTGCTATATTGTTGGAGCACTACAAAGGAAAGTGGCCCTTTTGGCTTAGTCCACGTCAAGCAATTGTTTGCCCTGTATCAGAGAAATCTCAATCCTATGCCATGAAGGTAGCACTTAGAGTATTATCTGTTTGATTTCCTTTACAATACCCAATCTGTTTTTCTAAGTCCCACTGTCAAAGTTTTAGTATTCAAAATTTTCAGACTAATATATCATTGGATCGCTTACTCGATACAATTTTCTTTTCATTAATGTAAATGTAGAGAGCCACTGACATGACTGCATGTTGTAATACAATACAAAGGAAATTGAGTACATGACATGTACCAACTTGCTGTTTTATATTTTGTAGGTGCGTGATGTAATCCATCATGCCGGTTATTATGTTGACGTGGATATAACAGACCGAAAGATTCAGAAGAAGGTGAGTCCAACGAGGTCTTTATTATTCCTGTTCATGCTTTTGTGCCTTTTCAAAATGAATATTTCTTACTTTCCTAGTTCTTCAACATAGAAGTGAATTGCCTACACCTAAGAAGCACGGGCACTTCAATTTTTGGCCATTGTGTCCGTGTCCAATATATGTTGGACATTTGGACACTCCGACACTTGTTGGACACATACTAGATACTTGTTAGCACAATAGATATGTGTTAGATACTTGTTAGGTCTAATATTTATTACACACGTAGTACGCCGTTGTTAAGTATACTAAATAGACACATAATAGTATATGATAAATAATAAATTTTGAAAGCGAAATACATCAAACTCATTTCTTTAAGCATATAAATGCATAAACTTGTTGATTTTGAATTTCCTTGTATGATATAGCGCTTTTGGATGGCACTTTGCTAGACCGGGGGACATCCAAACTCTTCATTATTCTCTTCTTGGTCACCCCTTCAATAACGGCACTAAGATTCTTTGGCGAAATTTTTTGTATGCATTCTTTTGGAACTTGTGGTTAGAGCGAAATGCTAGAATCTTCAATAATAAGCAGCAGAATATTTATGCTTTCATTGAATCCACATCATATCTTGCTATTTACTGGAGCAGTCTCAGTTCCCCCTTTTGTAACTACCCTTTATCTACCCTTATATCTCAATGGGGATCTCTTTTGTAATTATCGATAGGCCCCTTTTGTAATCTATTTCATACATCAATGAAATGATTCTGTTTCCTATAAAAAAAAAAGAAAAAAAGAAAATGATATATGTTTTAAAAAGTGTATATTTTAATAAACGTGTCTTAGCCGTGTCCGTGTCCTAGACTTTTAAAAAGTGGCATGTCACCGTGTCCGTGTCGTGTCATGTCCTATCCTTGTCTTGTATTCATATCCATGCTTATTAAGCCTACACTAATTTGTATGGTTATCGTTATTATATATTATGATGTTTACTCAAAACAATAGATGGATGAGTTCTTAATAGTTGGTTAAGATTAATAGATTCTTGTGAAAGAAATGATTATCTGGGTTTCTTGCAGGTGCGAGAAGCACAATTGGCACAATATAACTACATATTAGTCGTTGGTGAGGAGGAAGCTAACACTGAACAGGTCTTAATGCTTATAATGACTAAATAATATTTGTTTCGTTGCTTGTTTCCGCACTTTTAAGAACAAAATTTAACTTTAGTAAATTTTATGGTCCACATTTTTCTTATACAAATTCTCTTTTCATTTGTCAAAAATAGAATCTGTCGTAGCTAGTTTAGTTAAATGTGGCACTCAACCGTTGAGTTGTTGTGGCAGGTGAGTGTACGGGTGAGAGATAAGGGTGACCATTCAGTGATGAGTATTGAGAACCTGCTCGAACACTTCAAGGAAGAAGCTGCAGCTTTCCATTAGACTTGTTCATTTGAAAATATAGCACAAAGTAAGGGGTATTGTTGTCAGATATAAAATAAAGAAATTGTTACAAGAAAGGTTAGAATGCCTTCTCACCATCATTTACAACTCAACTGTTGTTTATCTCCCCCCTATACCAAAAGTATATCGGTTTCTTTGTCTGAGATTTTCTTCCCACATTGTCAATTGTGTAATATATTAATCTAATGGAGGAAAGTGTTTTAGTAGTTGGAGGCTTTGGTTTTGTGTAGAAGTATATAAATGTCTTTTAGTACAGAAGCCTACCGGCCATGGATGAAGTCGCCAAAACTACGAAAACTTCCTGTCAGAAAGTCTGGTAGTTTACTCTTTGCAAATTGTAGTAAATATCTGAGAAAGATCTCTATTTAAAAATGTCATTTAATTTGAAGTATTACAAG

mRNA sequence

ATGAGCGTTATCAAAAGAGACTTCTTTTGGGCTGGTGGAGATAACAGAGTTGGTAGCCACCTGGTGAATTGGGTTGTTGTTTCTCTTCCTCTTTGTCAAGGAGGTTTGGGGATTGGAGGCTCGAAGCATCGGAATGCAGCCCTTCTTACGAAGAACGAGGAAAATGCTTTTTGGAGGCAGGTGGTGGCTAGTATTTACGGGGAAGATCCTCATGGGTGGTTGGTTTCCCGTTTCACTAGAGCTCCAAGAATAGACCTTGGTTGGACATCGCTAAGATGGTGCCTCTATATGAGAAGTTCATCAGACGGGGATTCTCTTAATCATCTATTCTTTGGATGTTCTTTCTCAATGGCTTGTTGGTTCAAACTATTCAGTATCTTCAATTTGCAGTGGGTGTTTTCGTATTCTGCCAAAGATGATGTTCTTCAGCTTTCATTGGACTTATCTTTTACCTTTGAGACCTCATTTACTATGGATCAACACAGTCAAAGCTTTGATTTCAAAATTATGAGGCCAGAGAAATACCTTGGAGATTTGGAAACATGGGAGAAAGCTGAAGCTGCACTTACAGAAGCACTAAATGAATTCGGGAAGCCCTGGCAGATCAATGAAGGAGATGGTGCATTTTATGGACCGAAGATAGATATTAGTGTCTCCGATGCATTGAAAAGAAAATTTCAATGTGCAACCCTTCAGTTGGACTTCCAGCTGCCTTCTCGCTTTGATTTGTATTACTCAGCAGAGGATGAGGCCAAAAGGGAACGACCTGTTATGATTCATAGAGCAATCTTGGGGTCTGTAGAGAGAATGTTTGCTATATTGTTGGAGCACTACAAAGGAAAGTGGCCCTTTTGGCTTAGTCCACGTCAAGCAATTGTTTGCCCTGTATCAGAGAAATCTCAATCCTATGCCATGAAGGTGCGTGATGTAATCCATCATGCCGGTTATTATGTTGACGTGGATATAACAGACCGAAAGATTCAGAAGAAGGTGCGAGAAGCACAATTGGCACAATATAACTACATATTAGTCGTTGGTGAGGAGGAAGCTAACACTGAACAGGTGAGTGTACGGGTGAGAGATAAGGGTGACCATTCAGTGATGAGTATTGAGAACCTGCTCGAACACTTCAAGGAAGAAGCTGCAGCTTTCCATTAG

Coding sequence (CDS)

ATGAGCGTTATCAAAAGAGACTTCTTTTGGGCTGGTGGAGATAACAGAGTTGGTAGCCACCTGGTGAATTGGGTTGTTGTTTCTCTTCCTCTTTGTCAAGGAGGTTTGGGGATTGGAGGCTCGAAGCATCGGAATGCAGCCCTTCTTACGAAGAACGAGGAAAATGCTTTTTGGAGGCAGGTGGTGGCTAGTATTTACGGGGAAGATCCTCATGGGTGGTTGGTTTCCCGTTTCACTAGAGCTCCAAGAATAGACCTTGGTTGGACATCGCTAAGATGGTGCCTCTATATGAGAAGTTCATCAGACGGGGATTCTCTTAATCATCTATTCTTTGGATGTTCTTTCTCAATGGCTTGTTGGTTCAAACTATTCAGTATCTTCAATTTGCAGTGGGTGTTTTCGTATTCTGCCAAAGATGATGTTCTTCAGCTTTCATTGGACTTATCTTTTACCTTTGAGACCTCATTTACTATGGATCAACACAGTCAAAGCTTTGATTTCAAAATTATGAGGCCAGAGAAATACCTTGGAGATTTGGAAACATGGGAGAAAGCTGAAGCTGCACTTACAGAAGCACTAAATGAATTCGGGAAGCCCTGGCAGATCAATGAAGGAGATGGTGCATTTTATGGACCGAAGATAGATATTAGTGTCTCCGATGCATTGAAAAGAAAATTTCAATGTGCAACCCTTCAGTTGGACTTCCAGCTGCCTTCTCGCTTTGATTTGTATTACTCAGCAGAGGATGAGGCCAAAAGGGAACGACCTGTTATGATTCATAGAGCAATCTTGGGGTCTGTAGAGAGAATGTTTGCTATATTGTTGGAGCACTACAAAGGAAAGTGGCCCTTTTGGCTTAGTCCACGTCAAGCAATTGTTTGCCCTGTATCAGAGAAATCTCAATCCTATGCCATGAAGGTGCGTGATGTAATCCATCATGCCGGTTATTATGTTGACGTGGATATAACAGACCGAAAGATTCAGAAGAAGGTGCGAGAAGCACAATTGGCACAATATAACTACATATTAGTCGTTGGTGAGGAGGAAGCTAACACTGAACAGGTGAGTGTACGGGTGAGAGATAAGGGTGACCATTCAGTGATGAGTATTGAGAACCTGCTCGAACACTTCAAGGAAGAAGCTGCAGCTTTCCATTAG

Protein sequence

MSVIKRDFFWAGGDNRVGSHLVNWVVVSLPLCQGGLGIGGSKHRNAALLTKNEENAFWRQVVASIYGEDPHGWLVSRFTRAPRIDLGWTSLRWCLYMRSSSDGDSLNHLFFGCSFSMACWFKLFSIFNLQWVFSYSAKDDVLQLSLDLSFTFETSFTMDQHSQSFDFKIMRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDALKRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKWPFWLSPRQAIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQYNYILVVGEEEANTEQVSVRVRDKGDHSVMSIENLLEHFKEEAAAFH

Homology

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BLAST of Spg030774 vs. NCBI nr
Match: XP_022972266.1 (threonine--tRNA ligase, mitochondrial 1-like [Cucurbita maxima] >XP_022972267.1 threonine--tRNA ligase, mitochondrial 1-like [Cucurbita maxima])

HSP 1 Score: 431.4 bits (1108), Expect = 8.1e-117
Identity = 211/223 (94.62%), Postives = 217/223 (97.31%), Query Frame = 0


Query: 164 SFDFKI-MRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 223
           +F+ K+  RPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL
Sbjct: 503 TFELKLSTRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 562

Query: 224 KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW 283
           KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW
Sbjct: 563 KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW 622

Query: 284 PFWLSPRQAIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQYNYI 343
           PFWLSPRQAIVCPVSEKSQSYA+KVRD IHHAGYYVDVD TDRKIQKKVREAQLAQYNYI
Sbjct: 623 PFWLSPRQAIVCPVSEKSQSYALKVRDAIHHAGYYVDVDTTDRKIQKKVREAQLAQYNYI 682

Query: 344 LVVGEEEANTEQVSVRVRDKGDHSVMSIENLLEHFKEEAAAFH 386
           LVVGEEEANTEQVSVRVRDKGDHSVM+IENLL+HFKEE AAFH
Sbjct: 683 LVVGEEEANTEQVSVRVRDKGDHSVMNIENLLKHFKEEVAAFH 725

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BLAST of Spg030774 vs. NCBI nr
Match: KAG6597727.1 (Threonine--tRNA ligase, mitochondrial 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 430.3 bits (1105), Expect = 1.8e-116
Identity = 210/223 (94.17%), Postives = 217/223 (97.31%), Query Frame = 0


Query: 164 SFDFKI-MRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 223
           +F+ K+  RPEKYLGD+ETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL
Sbjct: 535 TFELKLSTRPEKYLGDVETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 594

Query: 224 KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW 283
           KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW
Sbjct: 595 KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW 654

Query: 284 PFWLSPRQAIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQYNYI 343
           PFWLSPRQAIVCPVSEKSQSYA+KVRD IHHAGYYVDVD TDRKIQKKVREAQLAQYNYI
Sbjct: 655 PFWLSPRQAIVCPVSEKSQSYALKVRDAIHHAGYYVDVDTTDRKIQKKVREAQLAQYNYI 714

Query: 344 LVVGEEEANTEQVSVRVRDKGDHSVMSIENLLEHFKEEAAAFH 386
           LVVGEEEANTEQVSVRVRDKGDHSVM+IENLL+HFKEE AAFH
Sbjct: 715 LVVGEEEANTEQVSVRVRDKGDHSVMNIENLLKHFKEEVAAFH 757

[Close]

BLAST of Spg030774 vs. NCBI nr
Match: KAG7029173.1 (Threonine--tRNA ligase, mitochondrial 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 430.3 bits (1105), Expect = 1.8e-116
Identity = 210/223 (94.17%), Postives = 217/223 (97.31%), Query Frame = 0


Query: 164 SFDFKI-MRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 223
           +F+ K+  RPEKYLGD+ETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL
Sbjct: 263 TFELKLSTRPEKYLGDVETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 322

Query: 224 KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW 283
           KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW
Sbjct: 323 KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW 382

Query: 284 PFWLSPRQAIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQYNYI 343
           PFWLSPRQAIVCPVSEKSQSYA+KVRD IHHAGYYVDVD TDRKIQKKVREAQLAQYNYI
Sbjct: 383 PFWLSPRQAIVCPVSEKSQSYALKVRDAIHHAGYYVDVDTTDRKIQKKVREAQLAQYNYI 442

Query: 344 LVVGEEEANTEQVSVRVRDKGDHSVMSIENLLEHFKEEAAAFH 386
           LVVGEEEANTEQVSVRVRDKGDHSVM+IENLL+HFKEE AAFH
Sbjct: 443 LVVGEEEANTEQVSVRVRDKGDHSVMNIENLLKHFKEEVAAFH 485

[Close]

BLAST of Spg030774 vs. NCBI nr
Match: XP_022152397.1 (threonine--tRNA ligase, mitochondrial 1-like isoform X1 [Momordica charantia])

HSP 1 Score: 429.9 bits (1104), Expect = 2.4e-116
Identity = 208/223 (93.27%), Postives = 219/223 (98.21%), Query Frame = 0


Query: 164 SFDFKI-MRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 223
           +F+ K+  RPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL
Sbjct: 491 TFELKLSTRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 550

Query: 224 KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW 283
           KRKFQCATLQLDFQLP+RFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHY+GKW
Sbjct: 551 KRKFQCATLQLDFQLPARFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYRGKW 610

Query: 284 PFWLSPRQAIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQYNYI 343
           PFWLSPRQAIVCPVSEKSQSYA+KVRD+IHHAGYYVDVD+TDRKIQKKVREAQLAQYNYI
Sbjct: 611 PFWLSPRQAIVCPVSEKSQSYALKVRDLIHHAGYYVDVDVTDRKIQKKVREAQLAQYNYI 670

Query: 344 LVVGEEEANTEQVSVRVRDKGDHSVMSIENLLEHFKEEAAAFH 386
           LVVGEEEANT+QVSVRVRDKGDHSVMSIENL++HFKEE AAFH
Sbjct: 671 LVVGEEEANTKQVSVRVRDKGDHSVMSIENLIKHFKEEVAAFH 713

[Close]

BLAST of Spg030774 vs. NCBI nr
Match: XP_022152398.1 (threonine--tRNA ligase, mitochondrial 1-like isoform X2 [Momordica charantia])

HSP 1 Score: 429.9 bits (1104), Expect = 2.4e-116
Identity = 208/223 (93.27%), Postives = 219/223 (98.21%), Query Frame = 0


Query: 164 SFDFKI-MRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 223
           +F+ K+  RPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL
Sbjct: 454 TFELKLSTRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 513

Query: 224 KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW 283
           KRKFQCATLQLDFQLP+RFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHY+GKW
Sbjct: 514 KRKFQCATLQLDFQLPARFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYRGKW 573

Query: 284 PFWLSPRQAIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQYNYI 343
           PFWLSPRQAIVCPVSEKSQSYA+KVRD+IHHAGYYVDVD+TDRKIQKKVREAQLAQYNYI
Sbjct: 574 PFWLSPRQAIVCPVSEKSQSYALKVRDLIHHAGYYVDVDVTDRKIQKKVREAQLAQYNYI 633

Query: 344 LVVGEEEANTEQVSVRVRDKGDHSVMSIENLLEHFKEEAAAFH 386
           LVVGEEEANT+QVSVRVRDKGDHSVMSIENL++HFKEE AAFH
Sbjct: 634 LVVGEEEANTKQVSVRVRDKGDHSVMSIENLIKHFKEEVAAFH 676

[Close]

BLAST of Spg030774 vs. ExPASy Swiss-Prot
Match: O04630 (Threonine--tRNA ligase, mitochondrial 1 OS=Arabidopsis thaliana OX=3702 GN=THRRS PE=1 SV=3)

HSP 1 Score: 345.9 bits (886), Expect = 5.9e-94
Identity = 169/214 (78.97%), Postives = 185/214 (86.45%), Query Frame = 0


Query: 171 RPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDALKRKFQCAT 230
           RPEKYLGDLETW+KAEA L EA+  FGKP  +NEGDGAFYGPKIDI+VSDA+ RKFQCAT
Sbjct: 495 RPEKYLGDLETWDKAEADLKEAIEAFGKPLVLNEGDGAFYGPKIDITVSDAMNRKFQCAT 554

Query: 231 LQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKWPFWLSPRQ 290
           LQLDFQLP RF+L Y+AEDEAK+ RPVMIHRA+LGSVERMFAILLEHYKGKWPFW+SPRQ
Sbjct: 555 LQLDFQLPIRFNLEYAAEDEAKKSRPVMIHRAVLGSVERMFAILLEHYKGKWPFWISPRQ 614

Query: 291 AIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQYNYILVVGEEEA 350
           AIVCP+SEKSQ YA KV+  I  AG+YVD D+TDRKI KKVREAQLAQYNYILVVGE EA
Sbjct: 615 AIVCPISEKSQQYAEKVQKQIKDAGFYVDADLTDRKIDKKVREAQLAQYNYILVVGETEA 674

Query: 351 NTEQVSVRVRDKGDHSVMSIENLLEHFKEEAAAF 385
            T QVSVRVRD   HSV SIE+LLE FK + A F
Sbjct: 675 ATGQVSVRVRDNAAHSVKSIEDLLEEFKAKTAEF 708

[Close]

BLAST of Spg030774 vs. ExPASy Swiss-Prot
Match: Q8GZ45 (Probable threonine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At1g17960 PE=2 SV=2)

HSP 1 Score: 271.9 bits (694), Expect = 1.1e-71
Identity = 136/218 (62.39%), Postives = 169/218 (77.52%), Query Frame = 0


Query: 171 RPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDALKRKFQCAT 230
           RP+ ++GDLETW KAE  L +AL++FGKPW I EGDGAFYGPKIDI+VSDA  RKFQCAT
Sbjct: 241 RPKDHIGDLETWAKAENDLEKALDDFGKPWVIKEGDGAFYGPKIDITVSDARNRKFQCAT 300

Query: 231 LQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKWPFWLSPRQ 290
           +QLDFQLP+ F L Y +E + + E PVMIH A+LGS+ERMFAILLEHYKG WPFWLSPRQ
Sbjct: 301 IQLDFQLPACFKLKYLSE-KNEMEAPVMIHSAVLGSIERMFAILLEHYKGIWPFWLSPRQ 360

Query: 291 AIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQYNYILVVGEEEA 350
           AIVC +SE   SYA +V+  I+  GYYVD+D +DR ++KKV +A+ A YNYILVVG++E 
Sbjct: 361 AIVCSLSEDCSSYAKQVQKQINEVGYYVDIDESDRSLRKKVADARDAPYNYILVVGQKEV 420

Query: 351 NTEQVSVRVRD----KGDHSVMSIENLLEHFKEEAAAF 385
            T QV+VR+R+    + D   MSIE+LL+ FK +   F
Sbjct: 421 ATGQVTVRLREDPEGRKDLPEMSIESLLDEFKFKTVNF 457

[Close]

BLAST of Spg030774 vs. ExPASy Swiss-Prot
Match: Q54J66 (Probable threonine--tRNA ligase 1, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=thrS1 PE=3 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.9e-68
Identity = 125/215 (58.14%), Postives = 160/215 (74.42%), Query Frame = 0


Query: 171 RPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDALKRKFQCAT 230
           RP+ +LG++  W+ AEA+L +ALN+FGKPW++N  DGAFYGPKIDI ++D LKR  QCAT
Sbjct: 495 RPDNFLGEIAQWDIAEASLEKALNKFGKPWKLNPKDGAFYGPKIDIHITDCLKRSHQCAT 554

Query: 231 LQLDFQLPSRFDLYYSAEDEAK-RERPVMIHRAILGSVERMFAILLEHYKGKWPFWLSPR 290
           +QLDFQLP RFDL Y ++D A+ ++RPV+IHRAILGSVERM AIL+EH  GKWP W+SPR
Sbjct: 555 IQLDFQLPIRFDLEYQSDDAAELKKRPVIIHRAILGSVERMMAILIEHTGGKWPLWVSPR 614

Query: 291 QAIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQYNYILVVGEEE 350
           QAIV  V++    Y  KV   I  AG+Y D+D +D+ I KKVREAQLAQYNYILVVG+EE
Sbjct: 615 QAIVVTVNKTHNEYGQKVCKEISDAGFYCDIDDSDKTISKKVREAQLAQYNYILVVGQEE 674

Query: 351 ANTEQVSVRVRDKGDHSVMSIENLLEHFKEEAAAF 385
            N   V+VR RD      +++ +L+  FK+    F
Sbjct: 675 INGNTVNVRTRDNVVRGSLTVNDLISEFKQLVKEF 709

[Close]

BLAST of Spg030774 vs. ExPASy Swiss-Prot
Match: P04801 (Threonine--tRNA ligase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=THS1 PE=1 SV=2)

HSP 1 Score: 261.2 bits (666), Expect = 1.9e-68
Identity = 125/223 (56.05%), Postives = 166/223 (74.44%), Query Frame = 0


Query: 165 FDFKI---MRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDA 224
           F+FK+    RPEKY+G +ETW+ AE+ L  AL ++G  W+IN GDGAFYGPKIDI +SDA
Sbjct: 499 FEFKMELSTRPEKYVGKIETWDAAESKLESALKKWGGNWEINAGDGAFYGPKIDIMISDA 558

Query: 225 LKRKFQCATLQLDFQLPSRFDLYYSAEDEAKR--ERPVMIHRAILGSVERMFAILLEHYK 284
           L+R  QCAT+QLDFQLP+RF+L + ++D+     ERPVMIHRAILGSVERM AIL EH+ 
Sbjct: 559 LRRWHQCATIQLDFQLPNRFELEFKSKDQDSESYERPVMIHRAILGSVERMTAILTEHFA 618

Query: 285 GKWPFWLSPRQAIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQY 344
           GKWPFWLSPRQ +V PV  K Q YA  VR+ +H AG+Y DVD+T   +QKKVR  Q+ +Y
Sbjct: 619 GKWPFWLSPRQVLVVPVGVKYQGYAEDVRNKLHDAGFYADVDLTGNTLQKKVRNGQMLKY 678

Query: 345 NYILVVGEEEANTEQVSVRVRD----KGDHSVMSIENLLEHFK 379
           N+I +VGE+E N + V++R RD    +G ++ +S+E +L+  +
Sbjct: 679 NFIFIVGEQEMNEKSVNIRNRDVMEQQGKNATVSVEEVLKQLR 721

[Close]

BLAST of Spg030774 vs. ExPASy Swiss-Prot
Match: P26639 (Threonine--tRNA ligase 1, cytoplasmic OS=Homo sapiens OX=9606 GN=TARS1 PE=1 SV=3)

HSP 1 Score: 248.1 bits (632), Expect = 1.7e-64
Identity = 119/219 (54.34%), Postives = 153/219 (69.86%), Query Frame = 0


Query: 165 FDFKI---MRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDA 224
           F FK+    RPEK+LGD+E W++AE  L  +LNEFG+ W++N GDGAFYGPKIDI + DA
Sbjct: 492 FSFKLNLSTRPEKFLGDIEVWDQAEKQLENSLNEFGEKWELNSGDGAFYGPKIDIQIKDA 551

Query: 225 LKRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGK 284
           + R  QCAT+QLDFQLP RF+L Y + D   ++RPV++HRAILGSVERM AIL E+Y GK
Sbjct: 552 IGRYHQCATIQLDFQLPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGK 611

Query: 285 WPFWLSPRQAIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDR-KIQKKVREAQLAQYN 344
           WPFWLSPRQ +V PV      YA KVR   H A +  D+D+     + KK+R AQLAQYN
Sbjct: 612 WPFWLSPRQVMVVPVGPTCDEYAQKVRQQFHDAKFMADIDLDPGCTLNKKIRNAQLAQYN 671

Query: 345 YILVVGEEEANTEQVSVRVRDKGDHSVMSIENLLEHFKE 380
           +ILVVGE+E  +  V++R RD   H   +I   +E  ++
Sbjct: 672 FILVVGEKEKISGTVNIRTRDNKVHGERTISETIERLQQ 710

[Close]

BLAST of Spg030774 vs. ExPASy TrEMBL
Match: A0A6J1I4C8 (Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111470850 PE=3 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 3.9e-117
Identity = 211/223 (94.62%), Postives = 217/223 (97.31%), Query Frame = 0


Query: 164 SFDFKI-MRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 223
           +F+ K+  RPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL
Sbjct: 503 TFELKLSTRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 562

Query: 224 KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW 283
           KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW
Sbjct: 563 KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW 622

Query: 284 PFWLSPRQAIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQYNYI 343
           PFWLSPRQAIVCPVSEKSQSYA+KVRD IHHAGYYVDVD TDRKIQKKVREAQLAQYNYI
Sbjct: 623 PFWLSPRQAIVCPVSEKSQSYALKVRDAIHHAGYYVDVDTTDRKIQKKVREAQLAQYNYI 682

Query: 344 LVVGEEEANTEQVSVRVRDKGDHSVMSIENLLEHFKEEAAAFH 386
           LVVGEEEANTEQVSVRVRDKGDHSVM+IENLL+HFKEE AAFH
Sbjct: 683 LVVGEEEANTEQVSVRVRDKGDHSVMNIENLLKHFKEEVAAFH 725

[Close]

BLAST of Spg030774 vs. ExPASy TrEMBL
Match: A0A6J1DG60 (Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111020133 PE=3 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 1.1e-116
Identity = 208/223 (93.27%), Postives = 219/223 (98.21%), Query Frame = 0


Query: 164 SFDFKI-MRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 223
           +F+ K+  RPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL
Sbjct: 491 TFELKLSTRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 550

Query: 224 KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW 283
           KRKFQCATLQLDFQLP+RFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHY+GKW
Sbjct: 551 KRKFQCATLQLDFQLPARFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYRGKW 610

Query: 284 PFWLSPRQAIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQYNYI 343
           PFWLSPRQAIVCPVSEKSQSYA+KVRD+IHHAGYYVDVD+TDRKIQKKVREAQLAQYNYI
Sbjct: 611 PFWLSPRQAIVCPVSEKSQSYALKVRDLIHHAGYYVDVDVTDRKIQKKVREAQLAQYNYI 670

Query: 344 LVVGEEEANTEQVSVRVRDKGDHSVMSIENLLEHFKEEAAAFH 386
           LVVGEEEANT+QVSVRVRDKGDHSVMSIENL++HFKEE AAFH
Sbjct: 671 LVVGEEEANTKQVSVRVRDKGDHSVMSIENLIKHFKEEVAAFH 713

[Close]

BLAST of Spg030774 vs. ExPASy TrEMBL
Match: A0A6J1DER8 (Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111020133 PE=3 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 1.1e-116
Identity = 208/223 (93.27%), Postives = 219/223 (98.21%), Query Frame = 0


Query: 164 SFDFKI-MRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 223
           +F+ K+  RPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL
Sbjct: 454 TFELKLSTRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 513

Query: 224 KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW 283
           KRKFQCATLQLDFQLP+RFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHY+GKW
Sbjct: 514 KRKFQCATLQLDFQLPARFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYRGKW 573

Query: 284 PFWLSPRQAIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQYNYI 343
           PFWLSPRQAIVCPVSEKSQSYA+KVRD+IHHAGYYVDVD+TDRKIQKKVREAQLAQYNYI
Sbjct: 574 PFWLSPRQAIVCPVSEKSQSYALKVRDLIHHAGYYVDVDVTDRKIQKKVREAQLAQYNYI 633

Query: 344 LVVGEEEANTEQVSVRVRDKGDHSVMSIENLLEHFKEEAAAFH 386
           LVVGEEEANT+QVSVRVRDKGDHSVMSIENL++HFKEE AAFH
Sbjct: 634 LVVGEEEANTKQVSVRVRDKGDHSVMSIENLIKHFKEEVAAFH 676

[Close]

BLAST of Spg030774 vs. ExPASy TrEMBL
Match: A0A6J1F237 (Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111439084 PE=3 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 2.0e-116
Identity = 209/223 (93.72%), Postives = 216/223 (96.86%), Query Frame = 0


Query: 164 SFDFKI-MRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDAL 223
           +F+ K+  RPEKYLGDLETWEKAEAALTEALNEFG PWQINEGDGAFYGPKIDISVSDAL
Sbjct: 504 TFELKLSTRPEKYLGDLETWEKAEAALTEALNEFGNPWQINEGDGAFYGPKIDISVSDAL 563

Query: 224 KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKW 283
           KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRA+LGSVERMFAILLEHYKGKW
Sbjct: 564 KRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAVLGSVERMFAILLEHYKGKW 623

Query: 284 PFWLSPRQAIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQYNYI 343
           PFWLSPRQAIVCPVSEKSQSYA+KVRD IHHAGYYVDVD TDRKIQKKVREAQLAQYNYI
Sbjct: 624 PFWLSPRQAIVCPVSEKSQSYALKVRDAIHHAGYYVDVDTTDRKIQKKVREAQLAQYNYI 683

Query: 344 LVVGEEEANTEQVSVRVRDKGDHSVMSIENLLEHFKEEAAAFH 386
           LVVGEEEANTEQVSVRVRDKGDHSVM+IENLL+HFKEE AAFH
Sbjct: 684 LVVGEEEANTEQVSVRVRDKGDHSVMNIENLLKHFKEEVAAFH 726

[Close]

BLAST of Spg030774 vs. ExPASy TrEMBL
Match: A0A6J1J5S0 (Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111481536 PE=3 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 4.4e-116
Identity = 210/231 (90.91%), Postives = 220/231 (95.24%), Query Frame = 0


Query: 156 FTMDQHSQSFDFKI-MRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKI 215
           +  D    +F+ K+  RPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKI
Sbjct: 489 YAYDIFGFTFELKLSTRPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKI 548

Query: 216 DISVSDALKRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAIL 275
           DISVSDALKRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAIL
Sbjct: 549 DISVSDALKRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAIL 608

Query: 276 LEHYKGKWPFWLSPRQAIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREA 335
           LEHYKGKWPFWLSPRQAIVCPVSEKSQSYA+KVRD+IH AG+YVDVD+TDRKIQKKVREA
Sbjct: 609 LEHYKGKWPFWLSPRQAIVCPVSEKSQSYALKVRDLIHDAGFYVDVDVTDRKIQKKVREA 668

Query: 336 QLAQYNYILVVGEEEANTEQVSVRVRDKGDHSVMSIENLLEHFKEEAAAFH 386
           QLAQYN+ILVVGEEEANTEQVSVRVRDKGDHSVMSIENLLEHFK+E AAFH
Sbjct: 669 QLAQYNFILVVGEEEANTEQVSVRVRDKGDHSVMSIENLLEHFKKEVAAFH 719

[Close]

BLAST of Spg030774 vs. TAIR 10
Match: AT5G26830.1 (Threonyl-tRNA synthetase )

HSP 1 Score: 345.9 bits (886), Expect = 4.2e-95
Identity = 169/214 (78.97%), Postives = 185/214 (86.45%), Query Frame = 0


Query: 171 RPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDALKRKFQCAT 230
           RPEKYLGDLETW+KAEA L EA+  FGKP  +NEGDGAFYGPKIDI+VSDA+ RKFQCAT
Sbjct: 495 RPEKYLGDLETWDKAEADLKEAIEAFGKPLVLNEGDGAFYGPKIDITVSDAMNRKFQCAT 554

Query: 231 LQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKWPFWLSPRQ 290
           LQLDFQLP RF+L Y+AEDEAK+ RPVMIHRA+LGSVERMFAILLEHYKGKWPFW+SPRQ
Sbjct: 555 LQLDFQLPIRFNLEYAAEDEAKKSRPVMIHRAVLGSVERMFAILLEHYKGKWPFWISPRQ 614

Query: 291 AIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQYNYILVVGEEEA 350
           AIVCP+SEKSQ YA KV+  I  AG+YVD D+TDRKI KKVREAQLAQYNYILVVGE EA
Sbjct: 615 AIVCPISEKSQQYAEKVQKQIKDAGFYVDADLTDRKIDKKVREAQLAQYNYILVVGETEA 674

Query: 351 NTEQVSVRVRDKGDHSVMSIENLLEHFKEEAAAF 385
            T QVSVRVRD   HSV SIE+LLE FK + A F
Sbjct: 675 ATGQVSVRVRDNAAHSVKSIEDLLEEFKAKTAEF 708

[Close]

BLAST of Spg030774 vs. TAIR 10
Match: AT1G17960.1 (Threonyl-tRNA synthetase )

HSP 1 Score: 271.9 bits (694), Expect = 7.7e-73
Identity = 136/218 (62.39%), Postives = 169/218 (77.52%), Query Frame = 0


Query: 171 RPEKYLGDLETWEKAEAALTEALNEFGKPWQINEGDGAFYGPKIDISVSDALKRKFQCAT 230
           RP+ ++GDLETW KAE  L +AL++FGKPW I EGDGAFYGPKIDI+VSDA  RKFQCAT
Sbjct: 241 RPKDHIGDLETWAKAENDLEKALDDFGKPWVIKEGDGAFYGPKIDITVSDARNRKFQCAT 300

Query: 231 LQLDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKWPFWLSPRQ 290
           +QLDFQLP+ F L Y +E + + E PVMIH A+LGS+ERMFAILLEHYKG WPFWLSPRQ
Sbjct: 301 IQLDFQLPACFKLKYLSE-KNEMEAPVMIHSAVLGSIERMFAILLEHYKGIWPFWLSPRQ 360

Query: 291 AIVCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQYNYILVVGEEEA 350
           AIVC +SE   SYA +V+  I+  GYYVD+D +DR ++KKV +A+ A YNYILVVG++E 
Sbjct: 361 AIVCSLSEDCSSYAKQVQKQINEVGYYVDIDESDRSLRKKVADARDAPYNYILVVGQKEV 420

Query: 351 NTEQVSVRVRD----KGDHSVMSIENLLEHFKEEAAAF 385
            T QV+VR+R+    + D   MSIE+LL+ FK +   F
Sbjct: 421 ATGQVTVRLREDPEGRKDLPEMSIESLLDEFKFKTVNF 457

[Close]

BLAST of Spg030774 vs. TAIR 10
Match: AT1G18130.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 191.4 bits (485), Expect = 1.3e-48
Identity = 100/152 (65.79%), Postives = 117/152 (76.97%), Query Frame = 0


Query: 233 LDFQLPSRFDLYYSAEDEAKRERPVMIHRAILGSVERMFAILLEHYKGKWPFWLSPRQAI 292
           +DF L  RF L YSAE+E   ER V+IHRA+L SVER+FAILLE YKGK+PFWLSPRQAI
Sbjct: 17  VDFHLRDRFKLEYSAENETMIERSVLIHRAVLSSVERLFAILLEQYKGKFPFWLSPRQAI 76

Query: 293 VCPVSEKSQSYAMKVRDVIHHAGYYVDVDITDRKIQKKVREAQLAQYNYILVVGEEEANT 352
           VC VSE  +SYA KVRD IH AGYYVDVD TDR +  KV+EAQ+A YNYILVVG+EEA T
Sbjct: 77  VCSVSEDYRSYADKVRDQIHEAGYYVDVDTTDRNVSDKVQEAQVAHYNYILVVGDEEART 136

Query: 353 EQVSVRVRDKGDHSVMSIENLLEHFKEEAAAF 385
            QV+V +RD+    +MSIE L + FK + A F
Sbjct: 137 GQVTVWLRDE----MMSIETLSDEFKLKTANF 164

[Close]

BLAST of Spg030774 vs. TAIR 10
Match: AT2G04842.1 (threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putative )

HSP 1 Score: 177.2 bits (448), Expect = 2.6e-44
Identity = 91/235 (38.72%), Postives = 146/235 (62.13%), Query Frame = 0


Query: 140 DVLQLSLDLSFTFETSFTMDQHSQSFDFKIMRPEKYLGDLETWEKAEAALTEALNEFGKP 199
           D ++  LDL+    + F  +++  +      RPEK +G  + WEKA  AL +AL++ G  
Sbjct: 408 DEIRGVLDLTEEILSRFGFNKYEVNLS---TRPEKSVGGDDIWEKATCALRDALDDKGWS 467

Query: 200 WQINEGDGAFYGPKIDISVSDALKRKFQCATLQLDFQLPSRFDLYYSAEDEAKRERPVMI 259
           ++++EG GAFYGPKID+ + DAL RK+QC+T+Q+DF LP RFD+ Y  +  + ++RP+MI
Sbjct: 468 YEVDEGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFDITY-VDTNSDKKRPIMI 527

Query: 260 HRAILGSVERMFAILLEHYKGKWPFWLSPRQAIVCPVSEKSQSYAMKVRDVIHHAGYYVD 319
           HRA+LGS+ER F +L+EHY G +P WLSP Q  V PV++    +  +V   +   G   +
Sbjct: 528 HRAVLGSLERFFGVLIEHYAGDFPLWLSPVQVRVLPVTDNQLEFCKEVSKKLRACGVRAE 587

Query: 320 VDITDRKIQKKVREAQLAQYNYILVVGEEEANTEQVSVRVRDKGDHSVMSIENLL 375
           +   +R + K +R A+  +   + VVG +E  T  V+VR R  G+   + +++L+
Sbjct: 588 LCHGER-LPKLIRNAETQKIPLMAVVGPKEVETGTVTVRSRFGGELGTIPVDDLI 637

The following BLAST results are available for this feature:

BLAST of Spg030774 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022972266.1	8.1e-117	94.62	threonine--tRNA ligase, mitochondrial 1-like [Cucurbita maxima] >XP_022972267.1 ...	[more]
KAG6597727.1	1.8e-116	94.17	Threonine--tRNA ligase, mitochondrial 1, partial [Cucurbita argyrosperma subsp. ...	[more]
KAG7029173.1	1.8e-116	94.17	Threonine--tRNA ligase, mitochondrial 1, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_022152397.1	2.4e-116	93.27	threonine--tRNA ligase, mitochondrial 1-like isoform X1 [Momordica charantia]	[more]
XP_022152398.1	2.4e-116	93.27	threonine--tRNA ligase, mitochondrial 1-like isoform X2 [Momordica charantia]	[more]

BLAST of Spg030774 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04630	5.9e-94	78.97	Threonine--tRNA ligase, mitochondrial 1 OS=Arabidopsis thaliana OX=3702 GN=THRRS...	[more]
Q8GZ45	1.1e-71	62.39	Probable threonine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q54J66	1.9e-68	58.14	Probable threonine--tRNA ligase 1, cytoplasmic OS=Dictyostelium discoideum OX=44...	[more]
P04801	1.9e-68	56.05	Threonine--tRNA ligase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204...	[more]
P26639	1.7e-64	54.34	Threonine--tRNA ligase 1, cytoplasmic OS=Homo sapiens OX=9606 GN=TARS1 PE=1 SV=3	[more]

BLAST of Spg030774 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I4C8	3.9e-117	94.62	Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111470850 PE=3 SV=1	[more]
A0A6J1DG60	1.1e-116	93.27	Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111020133 PE=3 SV=...	[more]
A0A6J1DER8	1.1e-116	93.27	Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111020133 PE=3 SV=...	[more]
A0A6J1F237	2.0e-116	93.72	Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111439084 PE=3 SV=1	[more]
A0A6J1J5S0	4.4e-116	90.91	Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111481536 PE=3 SV=1	[more]

BLAST of Spg030774 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G26830.1	4.2e-95	78.97	Threonyl-tRNA synthetase	[more]
AT1G17960.1	7.7e-73	62.39	Threonyl-tRNA synthetase	[more]
AT1G18130.1	1.3e-48	65.79	Class II aaRS and biotin synthetases superfamily protein	[more]
AT2G04842.1	2.6e-44	38.72	threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putative	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002320	Threonine-tRNA ligase, class IIa	PRINTS	PR01047	TRNASYNTHTHR	coord: 212..240 score: 56.4 coord: 256..269 score: 73.47 coord: 283..295 score: 56.87
IPR002320	Threonine-tRNA ligase, class IIa	PANTHER	PTHR11451	THREONINE-TRNA LIGASE	coord: 161..384
IPR002314	Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)	PFAM	PF00587	tRNA-synt_2b	coord: 192..278 e-value: 2.9E-10 score: 40.4
IPR036621	Anticodon-binding domain superfamily	GENE3D	3.40.50.800		coord: 282..384 e-value: 4.2E-38 score: 132.1
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 139..281 e-value: 1.2E-53 score: 184.2
None	No IPR available	PANTHER	PTHR11451:SF53	THREONINE--TRNA LIGASE, CYTOPLASMIC	coord: 161..384
None	No IPR available	CDD	cd00860	ThrRS_anticodon	coord: 288..378 e-value: 9.19147E-35 score: 121.457
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 171..275
None	No IPR available	SUPERFAMILY	52954	Class II aaRS ABD-related	coord: 276..381
IPR004154	Anticodon-binding	PFAM	PF03129	HGTP_anticodon	coord: 290..379 e-value: 2.1E-22 score: 79.1

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg030774.1	Spg030774.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006435	threonyl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004829	threonine-tRNA ligase activity
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding