Spg030683 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg030683 vs. NCBI nr
Match: XP_011653021.1 (uncharacterized protein LOC101222760 [Cucumis sativus] >KGN52991.1 hypothetical protein Csa_014932 [Cucumis sativus])

HSP 1 Score: 824.7 bits (2129), Expect = 4.6e-235
Identity = 432/553 (78.12%), Postives = 475/553 (85.90%), Query Frame = 0

Query: 14  MMRFRNGVKAMM-IPLVFLLVSSAWVFPESVGQKISSSSSLLQDERDLVQGNDGLEAIKE 73
           MM  +NGVK +M I LVFLLVSS+W+FPE++GQ+ISSS+SLLQD RD V+ NDGLEAIKE
Sbjct: 1   MMGSKNGVKVVMKIALVFLLVSSSWIFPETLGQEISSSNSLLQDGRDFVRKNDGLEAIKE 60

Query: 74  EDDTVRVDPLNHFKKYRGGYNITNKHYWSVSSLSSFSHGLGWIGLNSVNSTIFTGAAGYG 133
            DDTVRVDPLNHF KYRGGYNITNKHYWS                    ST+FTGA GYG
Sbjct: 61  ADDTVRVDPLNHFNKYRGGYNITNKHYWS--------------------STVFTGAVGYG 120

Query: 134 IGVVWLLCGVAYGGFLVATLCC-GKGKGKGKLKKMPHFGQKFYLWTILLASFFTILAIVG 193
           IGVVWL+CG+AYGGFLVATLCC GKG+GK KLKKMPH GQ+FYLWTILLA+FFTILAIVG
Sbjct: 121 IGVVWLVCGIAYGGFLVATLCCTGKGRGKRKLKKMPHLGQEFYLWTILLAAFFTILAIVG 180

Query: 194 CGLVIGGSSRFEREAKDVVKIIIETANGASNTIQNTTSAMKGLIANLEASRET-----EE 253
           CG+VIGGS+RF++EAK+VVKIIIETANGASNTIQNTTSAMK +I+NLEAS+ T     +E
Sbjct: 181 CGVVIGGSTRFDKEAKNVVKIIIETANGASNTIQNTTSAMKDMISNLEASKTTGSYRIQE 240

Query: 254 TSRTLTSTSRQLDAQAANIQSQANKNRLLIHKGLNIMYIVTMVTISLNLGAVIALSVFGI 313
           TS TLTSTS  LDAQAANIQ QANKNRLLIHKGLNI+YIVTMVT+SLNLGAVIA+SVFGI
Sbjct: 241 TSGTLTSTSHNLDAQAANIQWQANKNRLLIHKGLNIVYIVTMVTMSLNLGAVIAMSVFGI 300

Query: 314 LRLRKLFHI------------------------FSSDTCTALEMFQENPNNNSLSSILPC 373
           LRL++L H+                        FSSDTC ALEMFQENPNNNSLSSILPC
Sbjct: 301 LRLQRLLHLFILLCWILTVLCWIFFGLYLFLNNFSSDTCKALEMFQENPNNNSLSSILPC 360

Query: 374 EQLLTAKSVLTDVSSDIYDLVNKVNSQISVSYPDIALVCNPFSEPPYYEYQPQNCAANTI 433
           EQLLTAKSVLTDVSS+IYDLVN+VN+QI++SYPDIALVCNPFS+PPYYEYQPQNCAANTI
Sbjct: 361 EQLLTAKSVLTDVSSEIYDLVNQVNTQIAISYPDIALVCNPFSQPPYYEYQPQNCAANTI 420

Query: 434 RIGDIPKVLKLLTCSDGSNGGCENGQFMSNSEYKTVEAYTNSIQDFLNVYPGMESLVECQ 493
           RIGDIPKVLKLLTC+D +NGGCENGQFMSN EYKTVEAYTNSIQDFLNVYPGMESLVECQ
Sbjct: 421 RIGDIPKVLKLLTCADENNGGCENGQFMSNFEYKTVEAYTNSIQDFLNVYPGMESLVECQ 480

Query: 494 TVKEAFSKILEDHCKPLEKYAYMVWTGLVFVSVVMLCLVLIWTIRANVEQKLHLSDGSVQ 536
           TVK+AFSKILE HCKPLEKYAYMVW GLVFVS+VM+CLVLIWTIRAN++QKLH  DGSVQ
Sbjct: 481 TVKDAFSKILEHHCKPLEKYAYMVWVGLVFVSIVMVCLVLIWTIRANIDQKLHHFDGSVQ 533

BLAST of Spg030683 vs. NCBI nr
Match: XP_008454110.1 (PREDICTED: uncharacterized protein LOC103494614 [Cucumis melo])

HSP 1 Score: 822.0 bits (2122), Expect = 3.0e-234
Identity = 432/556 (77.70%), Postives = 478/556 (85.97%), Query Frame = 0

Query: 13  LMMRFRNGVKAMM-IPLVFLLVSSAWVFPESVGQKISSSSSLLQDERDLVQGNDGLEAIK 72
           ++M  RNGV+ +M I LVFLLVSS+W+FPE++GQ+I SS+SLLQD RD VQ NDGLEAIK
Sbjct: 1   MIMGSRNGVQVVMKIALVFLLVSSSWIFPETLGQEIPSSNSLLQDGRDFVQKNDGLEAIK 60

Query: 73  EEDDTVRVDPLNHFKKYRGGYNITNKHYWSVSSLSSFSHGLGWIGLNSVNSTIFTGAAGY 132
           EEDDTVRVDPLNHF KYR GYNITNKHYWS                    ST+FTGAAGY
Sbjct: 61  EEDDTVRVDPLNHFHKYRSGYNITNKHYWS--------------------STVFTGAAGY 120

Query: 133 GIGVVWLLCGVAYGGFL-VATLCCGKG-KGKGKLKKMPHFGQKFYLWTILLASFFTILAI 192
           GIGVVWL+CG+AYGGFL VATLCCGKG +GK KLKKMPH GQ+FYLWTILLA+FFTILAI
Sbjct: 121 GIGVVWLVCGIAYGGFLVVATLCCGKGSRGKPKLKKMPHLGQEFYLWTILLAAFFTILAI 180

Query: 193 VGCGLVIGGSSRFEREAKDVVKIIIETANGASNTIQNTTSAMKGLIANLEASRET----- 252
           VGCG+VIGGS+RF++EAK+VVKIIIETANGASNTIQNTTSAMK +I+NLEASR T     
Sbjct: 181 VGCGVVIGGSTRFDKEAKNVVKIIIETANGASNTIQNTTSAMKDMISNLEASRTTGRSYS 240

Query: 253 -EETSRTLTSTSRQLDAQAANIQSQANKNRLLIHKGLNIMYIVTMVTISLNLGAVIALSV 312
            +ETS TLTSTS  LDAQAANIQ QANKNRLLIHKGLNI+YIVTMVT+SLNLGAV+A++V
Sbjct: 241 IQETSGTLTSTSHNLDAQAANIQWQANKNRLLIHKGLNIVYIVTMVTMSLNLGAVVAMAV 300

Query: 313 FGILRLRKLFHI------------------------FSSDTCTALEMFQENPNNNSLSSI 372
           FGILRL++LFH+                        FSSDTC ALEMFQENPNNNSLSSI
Sbjct: 301 FGILRLQRLFHLFILLCWILTVLCWIFFGLYLFLNNFSSDTCKALEMFQENPNNNSLSSI 360

Query: 373 LPCEQLLTAKSVLTDVSSDIYDLVNKVNSQISVSYPDIALVCNPFSEPPYYEYQPQNCAA 432
           LPCEQLLTAKSVLTDVSS+IYDLVN+VN+QI++SYPDIALVCNPFS+PPYYEYQPQNCAA
Sbjct: 361 LPCEQLLTAKSVLTDVSSEIYDLVNQVNTQIAISYPDIALVCNPFSQPPYYEYQPQNCAA 420

Query: 433 NTIRIGDIPKVLKLLTCSDGSNGGCENGQFMSNSEYKTVEAYTNSIQDFLNVYPGMESLV 492
           NTIRIGDIPKVLKLLTC+D SNGGCENGQFMSN EY+TVEAYTNSIQDFLNVYPGME+LV
Sbjct: 421 NTIRIGDIPKVLKLLTCADESNGGCENGQFMSNFEYRTVEAYTNSIQDFLNVYPGMENLV 480

Query: 493 ECQTVKEAFSKILEDHCKPLEKYAYMVWTGLVFVSVVMLCLVLIWTIRANVEQKLHLSDG 536
           ECQTVK+AFSKILE HCKPLEKYAYMVW GLVFVS+VM+CLVLIWTIRAN++QKLH  DG
Sbjct: 481 ECQTVKDAFSKILEHHCKPLEKYAYMVWVGLVFVSIVMVCLVLIWTIRANIDQKLHHFDG 536

BLAST of Spg030683 vs. NCBI nr
Match: XP_022932737.1 (uncharacterized protein LOC111439196 isoform X2 [Cucurbita moschata])

HSP 1 Score: 788.1 bits (2034), Expect = 4.8e-224
Identity = 415/525 (79.05%), Postives = 447/525 (85.14%), Query Frame = 0

Query: 14  MMRFRNGVKAMMIPLVFLLVSSAWVFPESVGQKISSSSSLLQDERDLVQGNDGLEAIKEE 73
           MM  RNGVK  +IPL+++ +SS+W+FPE+VGQKISSSSSL+Q  RD VQ NDGLEA++EE
Sbjct: 1   MMGSRNGVKGFIIPLIWMFISSSWIFPETVGQKISSSSSLIQYGRDFVQRNDGLEALEEE 60

Query: 74  DDTVRVDPLNHFKKYRGGYNITNKHYWSVSSLSSFSHGLGWIGLNSVNSTIFTGAAGYGI 133
           D+TVRVDPLNHFK YRGGYNITNKHYWS                    STIFTGAAGYGI
Sbjct: 61  DNTVRVDPLNHFKMYRGGYNITNKHYWS--------------------STIFTGAAGYGI 120

Query: 134 GVVWLLCGVAYGGFLVATLCCGKGKGKGKLKKMPHFGQKFYLWTILLASFFTILAIVGCG 193
           GV WL+CG+AYGGF  ATLC GK + KGKLKKM H G KFYLWTILLA+FFTILA+VGCG
Sbjct: 121 GVAWLVCGIAYGGFFAATLCRGKRRSKGKLKKMSHCGDKFYLWTILLATFFTILALVGCG 180

Query: 194 LVIGGSSRFEREAKDVVKIIIETANGASNTIQNTTSAMKGLIANLEASR----ETEETSR 253
           LVI GSSRF+REAKDVVKIIIETANGASNTIQNTTSAMK +I NLEAS+      E  + 
Sbjct: 181 LVIVGSSRFDREAKDVVKIIIETANGASNTIQNTTSAMKDMIHNLEASKGIGTHEEAAAT 240

Query: 254 TLTSTSRQLDAQAANIQSQANKNRLLIHKGLNIMYIVTMVTISLNLGAVIALSVFGILRL 313
           TLTSTS +LDAQAANIQ QANKNR LIHKGLNIMYIVTMVTISLNLGAVI LSVFGILRL
Sbjct: 241 TLTSTSHKLDAQAANIQCQANKNRRLIHKGLNIMYIVTMVTISLNLGAVIGLSVFGILRL 300

Query: 314 RKLFHIFSSDTCTALEMFQENPNNNSLSSILPCEQLLTAKSVLTDVSSDIYDLVNKVNSQ 373
            +LFH FS D+C ALEMFQENPNNNSLSSILPCEQLLTAKS LTDVSS+IYDLVN+VN+Q
Sbjct: 301 HRLFHSFSIDSCRALEMFQENPNNNSLSSILPCEQLLTAKSALTDVSSEIYDLVNQVNTQ 360

Query: 374 ISVSYPDIALVCNPFSEPPYYEYQPQNCAANTIRIGDIPKVLKLLTCSDGSNGGCENGQF 433
           ISVSYPD  LVCNPFSEPPYYEYQP+NCAANTIRI DIPKVLKLLTCSD S+GGCEN QF
Sbjct: 361 ISVSYPDTGLVCNPFSEPPYYEYQPENCAANTIRIRDIPKVLKLLTCSDESHGGCENRQF 420

Query: 434 MSNSEYKTVEAYTNSIQDFLNVYPGMESLVECQTVKEAFSKILEDHCKPLEKYAYMVWTG 493
           MSNSEYKTVEAYTNS+QDF NVYPGMESLV CQTVK+AFSKILE HC+PLE YA MVW G
Sbjct: 421 MSNSEYKTVEAYTNSVQDFFNVYPGMESLVNCQTVKDAFSKILEHHCRPLESYANMVWAG 480

Query: 494 LVFVSVVMLCLVLIWTIRANVEQKLHLS-DGSVQPNSS-TPKMME 533
           LVFVSVVMLCLVLIWTI ANVE KLH S D SV PNSS TPKMME
Sbjct: 481 LVFVSVVMLCLVLIWTISANVEHKLHPSADASVLPNSSTTPKMME 505

BLAST of Spg030683 vs. NCBI nr
Match: XP_022972070.1 (uncharacterized protein LOC111470709 isoform X2 [Cucurbita maxima])

HSP 1 Score: 785.4 bits (2027), Expect = 3.1e-223
Identity = 412/527 (78.18%), Postives = 445/527 (84.44%), Query Frame = 0

Query: 14  MMRFRNGVKAMMIPLVFLLVSSAWVFPESVGQKISSSSSLLQDERDLVQGNDGLEAIKEE 73
           MM  RNGVK  +IPL+++ +SS+W+FPE+VGQKISSSSSL+Q  RD VQ NDGLEA++EE
Sbjct: 1   MMGSRNGVKGFIIPLIWMFISSSWIFPETVGQKISSSSSLIQYGRDSVQRNDGLEALEEE 60

Query: 74  DDTVRVDPLNHFKKYRGGYNITNKHYWSVSSLSSFSHGLGWIGLNSVNSTIFTGAAGYGI 133
           D+TVRVDPLNHFK YRGGYNITNKHYWS                    STIFTGA GYGI
Sbjct: 61  DNTVRVDPLNHFKMYRGGYNITNKHYWS--------------------STIFTGATGYGI 120

Query: 134 GVVWLLCGVAYGGFLVATLCCGKGKGKGKLKKMPHFGQKFYLWTILLASFFTILAIVGCG 193
           GV WL+CG+AYGGF   TLCCGK +GKGKLKK+ H G KFYLWT LLA+FFTILA+VGCG
Sbjct: 121 GVAWLVCGIAYGGFYAKTLCCGKRRGKGKLKKISHCGHKFYLWTFLLATFFTILAMVGCG 180

Query: 194 LVIGGSSRFEREAKDVVKIIIETANGASNTIQNTTSAMKGLIANLEASR----ETEETSR 253
           LVI GSSRF+REAKDVVKIIIETANGA NTIQNTTSAMK +I NLEAS+      E  + 
Sbjct: 181 LVIVGSSRFDREAKDVVKIIIETANGALNTIQNTTSAMKDMIHNLEASKGIGTHEEAAAT 240

Query: 254 TLTSTSRQLDAQAANIQSQANKNRLLIHKGLNIMYIVTMVTISLNLGAVIALSVFGILRL 313
           TLTSTS  LDAQAANIQ QANKNR LIHKGLNIMYI TMVTISLNLGAVI LSVFGILRL
Sbjct: 241 TLTSTSHTLDAQAANIQCQANKNRRLIHKGLNIMYIATMVTISLNLGAVIGLSVFGILRL 300

Query: 314 RKLFHIFSSDTCTALEMFQENPNNNSLSSILPCEQLLTAKSVLTDVSSDIYDLVNKVNSQ 373
            +LFH FS D+C ALEMFQENPNNNSLSSILPCEQLLTAKS LTDVSS+IYDLVN+VN+Q
Sbjct: 301 HRLFHSFSVDSCRALEMFQENPNNNSLSSILPCEQLLTAKSALTDVSSEIYDLVNQVNTQ 360

Query: 374 ISVSYPDIALVCNPFSEPPYYEYQPQNCAANTIRIGDIPKVLKLLTCSDGSNGGCENGQF 433
           ISVSYPDI LVCNPFSEPPYYEYQP+NCA NTIRI DIPKVLKLLTCSD SNGGCEN QF
Sbjct: 361 ISVSYPDIGLVCNPFSEPPYYEYQPENCAMNTIRIRDIPKVLKLLTCSDESNGGCENRQF 420

Query: 434 MSNSEYKTVEAYTNSIQDFLNVYPGMESLVECQTVKEAFSKILEDHCKPLEKYAYMVWTG 493
           MSNSEYKTVEAYTNS+QDF NVYPGMESLV CQTVK+AFSKILE HC+PLE YA MVW G
Sbjct: 421 MSNSEYKTVEAYTNSVQDFFNVYPGMESLVNCQTVKDAFSKILEHHCRPLESYANMVWAG 480

Query: 494 LVFVSVVMLCLVLIWTIRANVEQKLHLS-DGSVQPNSS-TPKMMEMA 535
           LVFVSVVMLCLVLIWTI ANVE KLH S D SV PNSS TP+MMEM+
Sbjct: 481 LVFVSVVMLCLVLIWTISANVEHKLHPSADASVLPNSSTTPRMMEMS 507

BLAST of Spg030683 vs. NCBI nr
Match: KAG6597688.1 (hypothetical protein SDJN03_10868, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 781.2 bits (2016), Expect = 5.8e-222
Identity = 416/551 (75.50%), Postives = 450/551 (81.67%), Query Frame = 0

Query: 14  MMRFRNGVKAMMIPLVFLLVSSAWVFPESVGQKISSSSSLLQDERDLVQGNDGLEAIKEE 73
           MM  RNGVK  +IPL+++ +SS+W+FPE+VGQKISSSSSL+Q  RD VQ NDGLEA++EE
Sbjct: 1   MMGSRNGVKGFIIPLIWMFISSSWIFPETVGQKISSSSSLIQYGRDFVQRNDGLEALEEE 60

Query: 74  DDTVRVDPLNHFKKYRGGYNITNKHYWSVSSLSSFSHGLGWIGLNSVNSTIFTGAAGYGI 133
           D+TVRVDPLNHFK YRGGYNITNKHYWS                    STIFTGAAGYGI
Sbjct: 61  DNTVRVDPLNHFKMYRGGYNITNKHYWS--------------------STIFTGAAGYGI 120

Query: 134 GVVWLLCGVAYGGFLVATLCCGKGKGKGKLKKMPHFGQKFYLWTILLASFFTILAIVGCG 193
           GV WL+CG+AYGGF  ATLCCGK + KGKLKKM H G KFYLWTILLA+FFTILA+VGCG
Sbjct: 121 GVAWLVCGIAYGGFFAATLCCGKRRSKGKLKKMSHCGDKFYLWTILLATFFTILALVGCG 180

Query: 194 LVIGGSSRFEREAKDVVKIIIETANGASNTIQNTTSAMKGLIANLEASR----ETEETSR 253
           LVI GSSRF+REAKDVVKIIIETANGASNTIQNTTSAMK +I NLEAS+      E  + 
Sbjct: 181 LVIVGSSRFDREAKDVVKIIIETANGASNTIQNTTSAMKDMIHNLEASKGIGTHEEAAAT 240

Query: 254 TLTSTSRQLDAQAANIQSQANKNRLLIHKGLNIMYIVTMVTISLNLGAVIALSVFGILRL 313
           TLTSTS +LDAQAANIQ QANKNR LIHKGLNIMYIVTMVTISLNLGAVI LSVFGILRL
Sbjct: 241 TLTSTSHKLDAQAANIQCQANKNRRLIHKGLNIMYIVTMVTISLNLGAVIGLSVFGILRL 300

Query: 314 RKLFHI------------------------FSSDTCTALEMFQENPNNNSLSSILPCEQL 373
            +LFH                         FS D+C ALEMFQENPNNNSLSSILPCEQL
Sbjct: 301 HRLFHSFILLCWFLTVVCWMFFGLYLFLNNFSIDSCRALEMFQENPNNNSLSSILPCEQL 360

Query: 374 LTAKSVLTDVSSDIYDLVNKVNSQISVSYPDIALVCNPFSEPPYYEYQPQNCAANTIRIG 433
           LTAKS LTDVSS+IYDLVN+VN+QIS+SYPD  LVCNPFSEPPYYEYQP+NCAANTIRI 
Sbjct: 361 LTAKSALTDVSSEIYDLVNQVNTQISISYPDTGLVCNPFSEPPYYEYQPENCAANTIRIR 420

Query: 434 DIPKVLKLLTCSDGSNGGCENGQFMSNSEYKTVEAYTNSIQDFLNVYPGMESLVECQTVK 493
           DIPKVLKLLTCSD S+GGCEN QFMSNSEYKTVEAYTNS+QDF NVYPGMESLV CQTVK
Sbjct: 421 DIPKVLKLLTCSDESHGGCENRQFMSNSEYKTVEAYTNSVQDFFNVYPGMESLVNCQTVK 480

Query: 494 EAFSKILEDHCKPLEKYAYMVWTGLVFVSVVMLCLVLIWTIRANVEQKLHLS-DGSVQPN 535
           +AFSKILE HC+PLE YA MVW GLVFVSVVMLCLVLIWTI ANVE KLH S D SV PN
Sbjct: 481 DAFSKILEHHCRPLESYANMVWAGLVFVSVVMLCLVLIWTISANVEHKLHPSADASVLPN 531

BLAST of Spg030683 vs. ExPASy TrEMBL
Match: A0A0A0KYT1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G010410 PE=4 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 2.2e-235
Identity = 432/553 (78.12%), Postives = 475/553 (85.90%), Query Frame = 0

Query: 14  MMRFRNGVKAMM-IPLVFLLVSSAWVFPESVGQKISSSSSLLQDERDLVQGNDGLEAIKE 73
           MM  +NGVK +M I LVFLLVSS+W+FPE++GQ+ISSS+SLLQD RD V+ NDGLEAIKE
Sbjct: 1   MMGSKNGVKVVMKIALVFLLVSSSWIFPETLGQEISSSNSLLQDGRDFVRKNDGLEAIKE 60

Query: 74  EDDTVRVDPLNHFKKYRGGYNITNKHYWSVSSLSSFSHGLGWIGLNSVNSTIFTGAAGYG 133
            DDTVRVDPLNHF KYRGGYNITNKHYWS                    ST+FTGA GYG
Sbjct: 61  ADDTVRVDPLNHFNKYRGGYNITNKHYWS--------------------STVFTGAVGYG 120

Query: 134 IGVVWLLCGVAYGGFLVATLCC-GKGKGKGKLKKMPHFGQKFYLWTILLASFFTILAIVG 193
           IGVVWL+CG+AYGGFLVATLCC GKG+GK KLKKMPH GQ+FYLWTILLA+FFTILAIVG
Sbjct: 121 IGVVWLVCGIAYGGFLVATLCCTGKGRGKRKLKKMPHLGQEFYLWTILLAAFFTILAIVG 180

Query: 194 CGLVIGGSSRFEREAKDVVKIIIETANGASNTIQNTTSAMKGLIANLEASRET-----EE 253
           CG+VIGGS+RF++EAK+VVKIIIETANGASNTIQNTTSAMK +I+NLEAS+ T     +E
Sbjct: 181 CGVVIGGSTRFDKEAKNVVKIIIETANGASNTIQNTTSAMKDMISNLEASKTTGSYRIQE 240

Query: 254 TSRTLTSTSRQLDAQAANIQSQANKNRLLIHKGLNIMYIVTMVTISLNLGAVIALSVFGI 313
           TS TLTSTS  LDAQAANIQ QANKNRLLIHKGLNI+YIVTMVT+SLNLGAVIA+SVFGI
Sbjct: 241 TSGTLTSTSHNLDAQAANIQWQANKNRLLIHKGLNIVYIVTMVTMSLNLGAVIAMSVFGI 300

Query: 314 LRLRKLFHI------------------------FSSDTCTALEMFQENPNNNSLSSILPC 373
           LRL++L H+                        FSSDTC ALEMFQENPNNNSLSSILPC
Sbjct: 301 LRLQRLLHLFILLCWILTVLCWIFFGLYLFLNNFSSDTCKALEMFQENPNNNSLSSILPC 360

Query: 374 EQLLTAKSVLTDVSSDIYDLVNKVNSQISVSYPDIALVCNPFSEPPYYEYQPQNCAANTI 433
           EQLLTAKSVLTDVSS+IYDLVN+VN+QI++SYPDIALVCNPFS+PPYYEYQPQNCAANTI
Sbjct: 361 EQLLTAKSVLTDVSSEIYDLVNQVNTQIAISYPDIALVCNPFSQPPYYEYQPQNCAANTI 420

Query: 434 RIGDIPKVLKLLTCSDGSNGGCENGQFMSNSEYKTVEAYTNSIQDFLNVYPGMESLVECQ 493
           RIGDIPKVLKLLTC+D +NGGCENGQFMSN EYKTVEAYTNSIQDFLNVYPGMESLVECQ
Sbjct: 421 RIGDIPKVLKLLTCADENNGGCENGQFMSNFEYKTVEAYTNSIQDFLNVYPGMESLVECQ 480

Query: 494 TVKEAFSKILEDHCKPLEKYAYMVWTGLVFVSVVMLCLVLIWTIRANVEQKLHLSDGSVQ 536
           TVK+AFSKILE HCKPLEKYAYMVW GLVFVS+VM+CLVLIWTIRAN++QKLH  DGSVQ
Sbjct: 481 TVKDAFSKILEHHCKPLEKYAYMVWVGLVFVSIVMVCLVLIWTIRANIDQKLHHFDGSVQ 533

BLAST of Spg030683 vs. ExPASy TrEMBL
Match: A0A1S3BZ25 (uncharacterized protein LOC103494614 OS=Cucumis melo OX=3656 GN=LOC103494614 PE=4 SV=1)

HSP 1 Score: 822.0 bits (2122), Expect = 1.4e-234
Identity = 432/556 (77.70%), Postives = 478/556 (85.97%), Query Frame = 0

Query: 13  LMMRFRNGVKAMM-IPLVFLLVSSAWVFPESVGQKISSSSSLLQDERDLVQGNDGLEAIK 72
           ++M  RNGV+ +M I LVFLLVSS+W+FPE++GQ+I SS+SLLQD RD VQ NDGLEAIK
Sbjct: 1   MIMGSRNGVQVVMKIALVFLLVSSSWIFPETLGQEIPSSNSLLQDGRDFVQKNDGLEAIK 60

Query: 73  EEDDTVRVDPLNHFKKYRGGYNITNKHYWSVSSLSSFSHGLGWIGLNSVNSTIFTGAAGY 132
           EEDDTVRVDPLNHF KYR GYNITNKHYWS                    ST+FTGAAGY
Sbjct: 61  EEDDTVRVDPLNHFHKYRSGYNITNKHYWS--------------------STVFTGAAGY 120

Query: 133 GIGVVWLLCGVAYGGFL-VATLCCGKG-KGKGKLKKMPHFGQKFYLWTILLASFFTILAI 192
           GIGVVWL+CG+AYGGFL VATLCCGKG +GK KLKKMPH GQ+FYLWTILLA+FFTILAI
Sbjct: 121 GIGVVWLVCGIAYGGFLVVATLCCGKGSRGKPKLKKMPHLGQEFYLWTILLAAFFTILAI 180

Query: 193 VGCGLVIGGSSRFEREAKDVVKIIIETANGASNTIQNTTSAMKGLIANLEASRET----- 252
           VGCG+VIGGS+RF++EAK+VVKIIIETANGASNTIQNTTSAMK +I+NLEASR T     
Sbjct: 181 VGCGVVIGGSTRFDKEAKNVVKIIIETANGASNTIQNTTSAMKDMISNLEASRTTGRSYS 240

Query: 253 -EETSRTLTSTSRQLDAQAANIQSQANKNRLLIHKGLNIMYIVTMVTISLNLGAVIALSV 312
            +ETS TLTSTS  LDAQAANIQ QANKNRLLIHKGLNI+YIVTMVT+SLNLGAV+A++V
Sbjct: 241 IQETSGTLTSTSHNLDAQAANIQWQANKNRLLIHKGLNIVYIVTMVTMSLNLGAVVAMAV 300

Query: 313 FGILRLRKLFHI------------------------FSSDTCTALEMFQENPNNNSLSSI 372
           FGILRL++LFH+                        FSSDTC ALEMFQENPNNNSLSSI
Sbjct: 301 FGILRLQRLFHLFILLCWILTVLCWIFFGLYLFLNNFSSDTCKALEMFQENPNNNSLSSI 360

Query: 373 LPCEQLLTAKSVLTDVSSDIYDLVNKVNSQISVSYPDIALVCNPFSEPPYYEYQPQNCAA 432
           LPCEQLLTAKSVLTDVSS+IYDLVN+VN+QI++SYPDIALVCNPFS+PPYYEYQPQNCAA
Sbjct: 361 LPCEQLLTAKSVLTDVSSEIYDLVNQVNTQIAISYPDIALVCNPFSQPPYYEYQPQNCAA 420

Query: 433 NTIRIGDIPKVLKLLTCSDGSNGGCENGQFMSNSEYKTVEAYTNSIQDFLNVYPGMESLV 492
           NTIRIGDIPKVLKLLTC+D SNGGCENGQFMSN EY+TVEAYTNSIQDFLNVYPGME+LV
Sbjct: 421 NTIRIGDIPKVLKLLTCADESNGGCENGQFMSNFEYRTVEAYTNSIQDFLNVYPGMENLV 480

Query: 493 ECQTVKEAFSKILEDHCKPLEKYAYMVWTGLVFVSVVMLCLVLIWTIRANVEQKLHLSDG 536
           ECQTVK+AFSKILE HCKPLEKYAYMVW GLVFVS+VM+CLVLIWTIRAN++QKLH  DG
Sbjct: 481 ECQTVKDAFSKILEHHCKPLEKYAYMVWVGLVFVSIVMVCLVLIWTIRANIDQKLHHFDG 536

BLAST of Spg030683 vs. ExPASy TrEMBL
Match: A0A6J1EXL4 (uncharacterized protein LOC111439196 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111439196 PE=4 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 2.3e-224
Identity = 415/525 (79.05%), Postives = 447/525 (85.14%), Query Frame = 0

Query: 14  MMRFRNGVKAMMIPLVFLLVSSAWVFPESVGQKISSSSSLLQDERDLVQGNDGLEAIKEE 73
           MM  RNGVK  +IPL+++ +SS+W+FPE+VGQKISSSSSL+Q  RD VQ NDGLEA++EE
Sbjct: 1   MMGSRNGVKGFIIPLIWMFISSSWIFPETVGQKISSSSSLIQYGRDFVQRNDGLEALEEE 60

Query: 74  DDTVRVDPLNHFKKYRGGYNITNKHYWSVSSLSSFSHGLGWIGLNSVNSTIFTGAAGYGI 133
           D+TVRVDPLNHFK YRGGYNITNKHYWS                    STIFTGAAGYGI
Sbjct: 61  DNTVRVDPLNHFKMYRGGYNITNKHYWS--------------------STIFTGAAGYGI 120

Query: 134 GVVWLLCGVAYGGFLVATLCCGKGKGKGKLKKMPHFGQKFYLWTILLASFFTILAIVGCG 193
           GV WL+CG+AYGGF  ATLC GK + KGKLKKM H G KFYLWTILLA+FFTILA+VGCG
Sbjct: 121 GVAWLVCGIAYGGFFAATLCRGKRRSKGKLKKMSHCGDKFYLWTILLATFFTILALVGCG 180

Query: 194 LVIGGSSRFEREAKDVVKIIIETANGASNTIQNTTSAMKGLIANLEASR----ETEETSR 253
           LVI GSSRF+REAKDVVKIIIETANGASNTIQNTTSAMK +I NLEAS+      E  + 
Sbjct: 181 LVIVGSSRFDREAKDVVKIIIETANGASNTIQNTTSAMKDMIHNLEASKGIGTHEEAAAT 240

Query: 254 TLTSTSRQLDAQAANIQSQANKNRLLIHKGLNIMYIVTMVTISLNLGAVIALSVFGILRL 313
           TLTSTS +LDAQAANIQ QANKNR LIHKGLNIMYIVTMVTISLNLGAVI LSVFGILRL
Sbjct: 241 TLTSTSHKLDAQAANIQCQANKNRRLIHKGLNIMYIVTMVTISLNLGAVIGLSVFGILRL 300

Query: 314 RKLFHIFSSDTCTALEMFQENPNNNSLSSILPCEQLLTAKSVLTDVSSDIYDLVNKVNSQ 373
            +LFH FS D+C ALEMFQENPNNNSLSSILPCEQLLTAKS LTDVSS+IYDLVN+VN+Q
Sbjct: 301 HRLFHSFSIDSCRALEMFQENPNNNSLSSILPCEQLLTAKSALTDVSSEIYDLVNQVNTQ 360

Query: 374 ISVSYPDIALVCNPFSEPPYYEYQPQNCAANTIRIGDIPKVLKLLTCSDGSNGGCENGQF 433
           ISVSYPD  LVCNPFSEPPYYEYQP+NCAANTIRI DIPKVLKLLTCSD S+GGCEN QF
Sbjct: 361 ISVSYPDTGLVCNPFSEPPYYEYQPENCAANTIRIRDIPKVLKLLTCSDESHGGCENRQF 420

Query: 434 MSNSEYKTVEAYTNSIQDFLNVYPGMESLVECQTVKEAFSKILEDHCKPLEKYAYMVWTG 493
           MSNSEYKTVEAYTNS+QDF NVYPGMESLV CQTVK+AFSKILE HC+PLE YA MVW G
Sbjct: 421 MSNSEYKTVEAYTNSVQDFFNVYPGMESLVNCQTVKDAFSKILEHHCRPLESYANMVWAG 480

Query: 494 LVFVSVVMLCLVLIWTIRANVEQKLHLS-DGSVQPNSS-TPKMME 533
           LVFVSVVMLCLVLIWTI ANVE KLH S D SV PNSS TPKMME
Sbjct: 481 LVFVSVVMLCLVLIWTISANVEHKLHPSADASVLPNSSTTPKMME 505

BLAST of Spg030683 vs. ExPASy TrEMBL
Match: A0A6J1I7I1 (uncharacterized protein LOC111470709 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470709 PE=4 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.5e-223
Identity = 412/527 (78.18%), Postives = 445/527 (84.44%), Query Frame = 0

Query: 14  MMRFRNGVKAMMIPLVFLLVSSAWVFPESVGQKISSSSSLLQDERDLVQGNDGLEAIKEE 73
           MM  RNGVK  +IPL+++ +SS+W+FPE+VGQKISSSSSL+Q  RD VQ NDGLEA++EE
Sbjct: 1   MMGSRNGVKGFIIPLIWMFISSSWIFPETVGQKISSSSSLIQYGRDSVQRNDGLEALEEE 60

Query: 74  DDTVRVDPLNHFKKYRGGYNITNKHYWSVSSLSSFSHGLGWIGLNSVNSTIFTGAAGYGI 133
           D+TVRVDPLNHFK YRGGYNITNKHYWS                    STIFTGA GYGI
Sbjct: 61  DNTVRVDPLNHFKMYRGGYNITNKHYWS--------------------STIFTGATGYGI 120

Query: 134 GVVWLLCGVAYGGFLVATLCCGKGKGKGKLKKMPHFGQKFYLWTILLASFFTILAIVGCG 193
           GV WL+CG+AYGGF   TLCCGK +GKGKLKK+ H G KFYLWT LLA+FFTILA+VGCG
Sbjct: 121 GVAWLVCGIAYGGFYAKTLCCGKRRGKGKLKKISHCGHKFYLWTFLLATFFTILAMVGCG 180

Query: 194 LVIGGSSRFEREAKDVVKIIIETANGASNTIQNTTSAMKGLIANLEASR----ETEETSR 253
           LVI GSSRF+REAKDVVKIIIETANGA NTIQNTTSAMK +I NLEAS+      E  + 
Sbjct: 181 LVIVGSSRFDREAKDVVKIIIETANGALNTIQNTTSAMKDMIHNLEASKGIGTHEEAAAT 240

Query: 254 TLTSTSRQLDAQAANIQSQANKNRLLIHKGLNIMYIVTMVTISLNLGAVIALSVFGILRL 313
           TLTSTS  LDAQAANIQ QANKNR LIHKGLNIMYI TMVTISLNLGAVI LSVFGILRL
Sbjct: 241 TLTSTSHTLDAQAANIQCQANKNRRLIHKGLNIMYIATMVTISLNLGAVIGLSVFGILRL 300

Query: 314 RKLFHIFSSDTCTALEMFQENPNNNSLSSILPCEQLLTAKSVLTDVSSDIYDLVNKVNSQ 373
            +LFH FS D+C ALEMFQENPNNNSLSSILPCEQLLTAKS LTDVSS+IYDLVN+VN+Q
Sbjct: 301 HRLFHSFSVDSCRALEMFQENPNNNSLSSILPCEQLLTAKSALTDVSSEIYDLVNQVNTQ 360

Query: 374 ISVSYPDIALVCNPFSEPPYYEYQPQNCAANTIRIGDIPKVLKLLTCSDGSNGGCENGQF 433
           ISVSYPDI LVCNPFSEPPYYEYQP+NCA NTIRI DIPKVLKLLTCSD SNGGCEN QF
Sbjct: 361 ISVSYPDIGLVCNPFSEPPYYEYQPENCAMNTIRIRDIPKVLKLLTCSDESNGGCENRQF 420

Query: 434 MSNSEYKTVEAYTNSIQDFLNVYPGMESLVECQTVKEAFSKILEDHCKPLEKYAYMVWTG 493
           MSNSEYKTVEAYTNS+QDF NVYPGMESLV CQTVK+AFSKILE HC+PLE YA MVW G
Sbjct: 421 MSNSEYKTVEAYTNSVQDFFNVYPGMESLVNCQTVKDAFSKILEHHCRPLESYANMVWAG 480

Query: 494 LVFVSVVMLCLVLIWTIRANVEQKLHLS-DGSVQPNSS-TPKMMEMA 535
           LVFVSVVMLCLVLIWTI ANVE KLH S D SV PNSS TP+MMEM+
Sbjct: 481 LVFVSVVMLCLVLIWTISANVEHKLHPSADASVLPNSSTTPRMMEMS 507

BLAST of Spg030683 vs. ExPASy TrEMBL
Match: A0A6J1EX81 (uncharacterized protein LOC111439196 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111439196 PE=4 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.6e-220
Identity = 415/549 (75.59%), Postives = 447/549 (81.42%), Query Frame = 0

Query: 14  MMRFRNGVKAMMIPLVFLLVSSAWVFPESVGQKISSSSSLLQDERDLVQGNDGLEAIKEE 73
           MM  RNGVK  +IPL+++ +SS+W+FPE+VGQKISSSSSL+Q  RD VQ NDGLEA++EE
Sbjct: 1   MMGSRNGVKGFIIPLIWMFISSSWIFPETVGQKISSSSSLIQYGRDFVQRNDGLEALEEE 60

Query: 74  DDTVRVDPLNHFKKYRGGYNITNKHYWSVSSLSSFSHGLGWIGLNSVNSTIFTGAAGYGI 133
           D+TVRVDPLNHFK YRGGYNITNKHYWS                    STIFTGAAGYGI
Sbjct: 61  DNTVRVDPLNHFKMYRGGYNITNKHYWS--------------------STIFTGAAGYGI 120

Query: 134 GVVWLLCGVAYGGFLVATLCCGKGKGKGKLKKMPHFGQKFYLWTILLASFFTILAIVGCG 193
           GV WL+CG+AYGGF  ATLC GK + KGKLKKM H G KFYLWTILLA+FFTILA+VGCG
Sbjct: 121 GVAWLVCGIAYGGFFAATLCRGKRRSKGKLKKMSHCGDKFYLWTILLATFFTILALVGCG 180

Query: 194 LVIGGSSRFEREAKDVVKIIIETANGASNTIQNTTSAMKGLIANLEASR----ETEETSR 253
           LVI GSSRF+REAKDVVKIIIETANGASNTIQNTTSAMK +I NLEAS+      E  + 
Sbjct: 181 LVIVGSSRFDREAKDVVKIIIETANGASNTIQNTTSAMKDMIHNLEASKGIGTHEEAAAT 240

Query: 254 TLTSTSRQLDAQAANIQSQANKNRLLIHKGLNIMYIVTMVTISLNLGAVIALSVFGILRL 313
           TLTSTS +LDAQAANIQ QANKNR LIHKGLNIMYIVTMVTISLNLGAVI LSVFGILRL
Sbjct: 241 TLTSTSHKLDAQAANIQCQANKNRRLIHKGLNIMYIVTMVTISLNLGAVIGLSVFGILRL 300

Query: 314 RKLFHI------------------------FSSDTCTALEMFQENPNNNSLSSILPCEQL 373
            +LFH                         FS D+C ALEMFQENPNNNSLSSILPCEQL
Sbjct: 301 HRLFHSFILLCWFLTVVCWMFFGLYLFLNNFSIDSCRALEMFQENPNNNSLSSILPCEQL 360

Query: 374 LTAKSVLTDVSSDIYDLVNKVNSQISVSYPDIALVCNPFSEPPYYEYQPQNCAANTIRIG 433
           LTAKS LTDVSS+IYDLVN+VN+QISVSYPD  LVCNPFSEPPYYEYQP+NCAANTIRI 
Sbjct: 361 LTAKSALTDVSSEIYDLVNQVNTQISVSYPDTGLVCNPFSEPPYYEYQPENCAANTIRIR 420

Query: 434 DIPKVLKLLTCSDGSNGGCENGQFMSNSEYKTVEAYTNSIQDFLNVYPGMESLVECQTVK 493
           DIPKVLKLLTCSD S+GGCEN QFMSNSEYKTVEAYTNS+QDF NVYPGMESLV CQTVK
Sbjct: 421 DIPKVLKLLTCSDESHGGCENRQFMSNSEYKTVEAYTNSVQDFFNVYPGMESLVNCQTVK 480

Query: 494 EAFSKILEDHCKPLEKYAYMVWTGLVFVSVVMLCLVLIWTIRANVEQKLHLS-DGSVQPN 533
           +AFSKILE HC+PLE YA MVW GLVFVSVVMLCLVLIWTI ANVE KLH S D SV PN
Sbjct: 481 DAFSKILEHHCRPLESYANMVWAGLVFVSVVMLCLVLIWTISANVEHKLHPSADASVLPN 529

BLAST of Spg030683 vs. TAIR 10
Match: AT5G67550.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: flower; EXPRESSED DURING: 4 anthesis; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G71110.1); Has 161 Blast hits to 154 proteins in 16 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 161; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 164.5 bits (415), Expect = 2.4e-40
Identity = 123/490 (25.10%), Postives = 220/490 (44.90%), Query Frame = 0

Query: 78  RVDPLNHFKKYRGGYNITNKHYWSVSSLSSFSHGLGWIGLNSVNSTIFTGAAGYGIGVVW 137
           R DPLN F+ Y GG+N+ NKHYW+                    +T FTG  GY +  V 
Sbjct: 39  RRDPLNSFRYYDGGFNVRNKHYWA--------------------ATAFTGIHGYAVAGVL 98

Query: 138 LLCGVAYGGFLVATLCCGKGKGKGKLKKMPHFGQKFYLWTILLASFFTILAIVGCGLVIG 197
           ++ G+  G ++  +      K +        +  ++YL   LL   F  L++V  G+VI 
Sbjct: 99  IIVGICLGLYVAFS-----DKRRRVSSTRRRYLDRYYLPLFLLLLLFMFLSVVTTGIVIA 158

Query: 198 GSSRFEREAKDVVKIIIETANGASNTIQNTTSAMKGLIANLEASRETEETSRTLTSTSRQ 257
            + R +   +++ + I +     +  I+    ++  +   L      + T+  L  T+ +
Sbjct: 159 ANQRSKNRTEEMKETIDKAGEDVNQNIRTVIVSLTKIQYLL--LPYDQNTTHLLNVTTHR 218

Query: 258 LDAQAANIQSQANKNRLLIHKGLNIMYIVTMVTISLNL----------------GAVIAL 317
           L   +  IQS  +     I   + I Y+  ++  S NL                G ++ +
Sbjct: 219 LGKGSRLIQSFLHHKGRSIDLAIKISYVSHLMITSTNLFLLLLAFLPLLLHWHPGFIMVI 278

Query: 318 SVFGILR--------LRKLFHIFSSDTCTALEMFQENPNNNSLSSILPCEQLLTAKSVLT 377
            +  I+              H F+ D C+A   F +NP N++L+++ PC   L +   L 
Sbjct: 279 FLCWIITTLCWVLTGFDFFIHTFAEDLCSAFNGFVQNPRNSTLTNLFPCMDPLHSDKTLI 338

Query: 378 DVSSDIYDLVNKVNSQISVSY---------------PDIALVCNPFSEPPYYEYQPQNCA 437
           ++S  I++ + ++NS+++ S                P+  ++C+PF       Y PQ+C+
Sbjct: 339 EISLMIHNFITQLNSKVAESMRSNALTDRSNTVSWAPESGIICDPFVGQQINSYTPQSCS 398

Query: 438 ANTIRIGDIPKVLKLLTCSD-GSNGGCE-NGQFMSNSEYKTVEAYTNSIQDFLNVYPGME 497
              I IG+ P +L   TC D      C   G+F+  + Y  V AY+NS Q  L++ P  +
Sbjct: 399 NGAIPIGEFPNILSRFTCHDKDPPETCRITGKFIPEAAYLKVYAYSNSAQGMLDILPSFQ 458

Query: 498 SLVECQTVKEAFSKILEDHCKPLEKYAYMVWTGLVFVSVVMLCLVLIWTIRANVEQKLHL 527
           +L EC  VK+  S I+ + C P     Y +W  ++ +S++M+ LVL++  +A  E+    
Sbjct: 459 NLTECLAVKDTLSSIVSNQCDPFRASMYRLWASILALSLIMVVLVLLFLAKAFQEKGKSF 501

BLAST of Spg030683 vs. TAIR 10
Match: AT2G12400.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G25270.1); Has 177 Blast hits to 172 proteins in 23 species: Archae - 0; Bacteria - 2; Metazoa - 3; Fungi - 0; Plants - 164; Viruses - 0; Other Eukaryotes - 8 (source: NCBI BLink). )

HSP 1 Score: 121.3 bits (303), Expect = 2.3e-27
Identity = 113/477 (23.69%), Postives = 199/477 (41.72%), Query Frame = 0

Query: 76  TVRVDPLNHFKKYRGGYNITNKHYWSVSSLSSFSHGLGWIGLNSVNSTIFTGAAGYGIGV 135
           T R DP ++FK Y GG+NI+N HY                    + S  +T A    I +
Sbjct: 72  TRRKDPADNFKLYTGGWNISNSHY--------------------LTSVGYTAAPFIIIAL 131

Query: 136 VW-LLCGVAYGGFLVATLCCGKGKGKGKLKKMPHFGQKFYLWTILLASFFTILAIVGCGL 195
           VW +  G++     +   CC         ++   + +  Y  +++L   FTI AI+GC  
Sbjct: 132 VWFVFFGLSLSLICLCYCCCA--------RQSYGYSRVAYALSLILLISFTIAAIIGCVF 191

Query: 196 VIGGSSRFEREAKDVVKIIIETANGASNTIQNTTSAMKGL-IANLEASRETEETSRTLTS 255
           +  G  +F     D +  ++  AN  S  ++N +  +      ++++S   ++   ++ +
Sbjct: 192 LYTGQGKFHASTTDTLDYVVSQANLTSENLRNVSDYLNAAKKVDVQSSILPQDVLSSIDN 251

Query: 256 TSRQLDAQAANIQSQANKNRLLIHKGLNIM--YIVTMVTISLNLGAV-IALSVFGILRL- 315
              ++++ A  +  +  +N+  I   L+IM   +V +  + L L  +   LS+FG+  L 
Sbjct: 252 IQGKINSSATTLSVKTMENQDKIQNVLDIMRLALVIIAAVMLFLAFIGFLLSIFGLQCLV 311

Query: 316 --------------------RKLFHIFSSDTCTALEMFQENPN-NNSLSSILPCEQLLTA 375
                                 L H    DTC A++ + +NP  + +L  ILPC    TA
Sbjct: 312 YTLVILGWILVTVTFVLCGGFLLLHNVVGDTCVAMDQWVQNPTAHTALDDILPCVDNATA 371

Query: 376 KSVLTDVSSDIYDLVNKVNSQIS-----------------VSYPDIALVCNPFSEPPYYE 435
           +  LT      Y LVN +++ IS                  S P + L+CNPF+     +
Sbjct: 372 RETLTRTKLVTYQLVNLLDNAISNMTNRNFPPQFRPLYYNQSGPLMPLLCNPFNA----D 431

Query: 436 YQPQNCAANTIRIGDIPKVLKLLTCSDGSNGGCENGQFMSNSEYKTVEAYTNSIQDFLNV 495
              + C    + + +  +V K  TC   + G C     ++   Y  + A  N        
Sbjct: 432 LSDRQCQPGQVHLNNATEVWKNFTCQIVTPGTCSTPGRLTPKLYSQMAAAVNVSYGLYKY 491

Query: 496 YPGMESLVECQTVKEAFSKILEDHCKPLEKYAYMVWTGLVFVSVVMLCLVLIWTIRA 509
            P +  L  C  V+  F+ I  DHC  L++Y   ++ GLV VS  ++  ++ W I A
Sbjct: 492 GPFLADLQGCDFVRSTFTDIERDHCPGLKRYTQWIYVGLVVVSASVMSSLVFWVIYA 516

BLAST of Spg030683 vs. TAIR 10
Match: AT2G25270.1 (unknown protein; LOCATED IN: plasma membrane; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G12400.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 119.4 bits (298), Expect = 8.9e-27
Identity = 104/477 (21.80%), Postives = 201/477 (42.14%), Query Frame = 0

Query: 76  TVRVDPLNHFKKYRGGYNITNKHYWSVSSLSSFSHGLGWIGLNSVNSTIFTGAAGYGIGV 135
           T R DPLN F+KY GG+NI+N+HYW+                    S  +T    + +  
Sbjct: 77  TYRKDPLNGFEKYTGGWNISNQHYWA--------------------SVSYTAVPLFVLAA 136

Query: 136 VWLL-CGVAYGGFLVATLC--CGKGKGKGKLKKMPHFGQKFYLWTILLASFFTILAIVGC 195
           VW L  G+     LV  +C  C +    G       + +  Y+ +++    FT++AI+GC
Sbjct: 137 VWFLGFGIC---LLVICMCHICHRTNSVG-------YSKVAYVVSLIFLLIFTVIAIIGC 196

Query: 196 GLVIGGSSRFEREAKDVVKIIIETANGASNTIQNTT--------SAMKGLIANLEASRET 255
            L+  G  R+ +   + ++ ++  A+   + ++  +        +A+  ++       E 
Sbjct: 197 VLLYSGQIRYNKSTTETLEYVMSQADSTISQLRAISDYLASAKQAAVLQVLLPANVQTEI 256

Query: 256 EETSRTLTSTSRQLDAQAANIQSQANKNRLLIHKGLNIMYIVTMVTISLN---------- 315
           ++    L S+   +  ++ N  +        +   L ++ IV +V   L           
Sbjct: 257 DQIGVKLDSSVATITEKSTNSSNHIRHFLDSVRVALIVVSIVMLVVTFLGLVSSIFGMQV 316

Query: 316 -------LGAVIALSVFGILRLRKLFHIFSSDTCTALEMFQENPNNN-SLSSILPCEQLL 375
                  LG ++    F +     + H  ++DTC A+  + E P++N +L  ILPC    
Sbjct: 317 IVYTLVILGWILVTGTFILSGTFLVLHNATADTCVAMSEWVERPSSNTALDEILPCTDNA 376

Query: 376 TAKSVL---TDVSSDIYDLVNKVNSQIS--------------VSYPDIALVCNPFSEPPY 435
           TA+  L    +V+  + +L+N V + +S               S P + L+CNPF+    
Sbjct: 377 TAQETLMRSREVTGQLVELINTVITNVSNINFSPVFVPMYYNQSGPLLPLLCNPFN---- 436

Query: 436 YEYQPQNCAANTIRIGDIPKVLKLLTCSDGSNGGCENGQFMSNSEYKTVEAYTNSIQDFL 495
           ++   ++C+   + + +  +      C    NG C     ++ + Y  + +  N     +
Sbjct: 437 HDLTDRSCSPGDLDLNNATEAWTSFVCQVSQNGTCTTTGRLTPALYSQMASGVNISTGLI 496

Query: 496 NVYPGMESLVECQTVKEAFSKILEDHCKPLEKYAYMVWTGLVFVSVVMLCLVLIWTI 507
              P +  L +C   K+ F  I  DHC  L++Y Y V+ GL  ++  ++  ++ W I
Sbjct: 497 RDAPFLVQLQDCSYAKQTFRDITNDHCPGLQRYGYWVYVGLAILATAVMLSLMFWII 519

BLAST of Spg030683 vs. TAIR 10
Match: AT1G71110.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G12400.1); Has 173 Blast hits to 169 proteins in 21 species: Archae - 0; Bacteria - 0; Metazoa - 3; Fungi - 0; Plants - 165; Viruses - 0; Other Eukaryotes - 5 (source: NCBI BLink). )

HSP 1 Score: 111.3 bits (277), Expect = 2.4e-24
Identity = 138/543 (25.41%), Postives = 212/543 (39.04%), Query Frame = 0

Query: 42  SVGQKISSSSSLLQDERDLVQGNDGLEAIK---------EEDDTV------------RVD 101
           S+   + SS S  QD   L+ G+      K         E DD V            R D
Sbjct: 18  SLPHSVDSSVSASQDPLRLILGSPNFGTWKGGISLAPGPESDDVVSDYLLLAAHRTKRPD 77

Query: 102 PLNHFKKYRGGYNITNKHYWSVSSLSSFSHGLGWIGLNSVNSTIFTGAAGYGIGVVWLLC 161
            L  FK Y GG+NITN HYW+                    S  FTGA G+ + V+WLL 
Sbjct: 78  ILRAFKPYHGGWNITNNHYWA--------------------SVGFTGAPGFILAVIWLL- 137

Query: 162 GVAYGGFLVATLC-----CGKGKGKGKLKKMPHFGQKFYLWTILLASFFTILAIVGCGLV 221
             ++G  LV   C     C K KG         F  +   + +L+   FT +A VGC L+
Sbjct: 138 --SFGSLLVVYHCFKWRICDKAKGSS-------FDTRRICFILLIV--FTCVAAVGCILL 197

Query: 222 IGGSSRFEREAKDVVKIIIETANGASNTIQNTTSAMK-GLIANLEASRETEETSRTLTST 281
             G  +F  EA   +K ++  ++     +QN T  +      N+       +    +   
Sbjct: 198 SVGQDKFHTEAMHTLKYVVNQSDYTVEILQNVTQYLSLAKTINVTQIVIPSDVMGEIDKL 257

Query: 282 SRQLDAQAANIQSQANKNRLLIHKGLNIM--YIVTMVTISLNLGAVIALSVFGILRLRKL 341
           +  L+  A  +      N   I +    +   ++T+ T+ L L  V  L    +LR + +
Sbjct: 258 NVNLNTAAVTLGETTTDNAAKIKRVFYAVRSALITVATVMLILSFVGLL--LSVLRHQHV 317

Query: 342 FHIF------------------------SSDTCTALEMFQENPN-NNSLSSILPCEQLLT 401
            HIF                         SDTC A++ + +NP+   +LSSILPC    T
Sbjct: 318 VHIFVVSGWILVAVTFVLCGVFLILNNAISDTCVAMKEWVDNPHAETALSSILPCVDQQT 377

Query: 402 AKSVLTDVSSDIYDLVNKVNSQI----------------SVSYPDIALVCNPFSEPPYYE 461
               L+     I  +V  VN+ +                + S P +  +C PF       
Sbjct: 378 TNQTLSQSKVVINSIVTVVNTFVYAVANTNPAPGQDRYYNQSGPPMPPLCIPFDA----N 437

Query: 462 YQPQNCAANTIRIGDIPKVLKLLTCSDGSNGGCENGQFMSNSEYKTVEAYTNSIQDFLNV 514
            + + C+   + I +   V +   C    +G C     ++   +  + A  N      + 
Sbjct: 438 MEDRQCSPWELSIENASSVWENYKCEVTPSGICTTVGRVTPDTFGQLVAAVNESYALEHY 497

BLAST of Spg030683 vs. TAIR 10
Match: AT1G80540.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G12400.1); Has 175 Blast hits to 171 proteins in 20 species: Archae - 0; Bacteria - 0; Metazoa - 2; Fungi - 0; Plants - 171; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 90.1 bits (222), Expect = 5.8e-18
Identity = 115/568 (20.25%), Postives = 223/568 (39.26%), Query Frame = 0

Query: 22  KAMMIPLVFLLVSSAWVFPESVGQKI---SSSSSLLQDERDLVQGNDGLEAIKEEDDTVR 81
           + ++I +  L+ SS   F  +V        SS+S     R +++G +G   +   + T R
Sbjct: 14  RLLLITVGLLITSSLVSFSHAVSSSCHHRPSSTSFQGVRRQILEGGNGTRLVLAAERTQR 73

Query: 82  VDPLNHFKKYRGGYNITNKHYWSVSSLSSFSHGLGWIGLNSVNSTIFTGAAGYGIGVVWL 141
            DPLNHF  Y  G+N+TN HY            +  +G ++V   +        I + W 
Sbjct: 74  PDPLNHFNIYVDGWNVTNSHY------------IASVGFSAVPFIV--------IAIAWF 133

Query: 142 LCGVAYGGFLVAT----LCCGKGKGKGKLKKMPHFGQKFYLWTILLASFFTILAIVGCGL 201
              V  G FL+ +     CCG G      ++   + +  Y  +++    FTI A++G  +
Sbjct: 134 ---VLLGLFLICSCLCCCCCGCG------RRNYGYSRVCYTLSLVFLLLFTIAAVIGSAM 193

Query: 202 VIGGSSRFEREAKDVVKIIIETANGASNTIQNTTSAMKG---------------LIANLE 261
           +  G + F    +     I++ A G    + +   +++                   N++
Sbjct: 194 LYTGQNEFYGSVERTFMYIVKQATGVLTKLTSLWDSIQSAKDIQLDGHNLFPPEFRGNID 253

Query: 262 ASRETEETSRTLTSTSRQLDAQAANIQSQANKNRLLIHKGLNIMYIV------------- 321
                 + S  +T   R  +     +    N  R +++    +M  V             
Sbjct: 254 HFNNMIKMS-NITYPDRVANQTIRYLTGALNPVRYVLNVIAGVMLAVAFLGLLFSFCGLR 313

Query: 322 TMVTISLNLGAVIALSVFGILRLRKLFHIFSSDTCTALEMFQENP-NNNSLSSILPCEQL 381
            +V + + LG ++  +   +  +  +FH   +DTC A++ +  +P  +++LS +LPC   
Sbjct: 314 VLVYLLVILGWILVTATILLSAVFLVFHNVVADTCMAMDQWVHDPAADSALSQLLPCLDP 373

Query: 382 LTAKSVL---TDVSSDIYDLVNKVNSQIS--------------VSYPDIALVCNPFSEPP 441
            T    L     +++   D+ N     +S               S P + L+CNP  +  
Sbjct: 374 KTIGETLDITKTMTATAVDMTNAYTVNVSNHDQFPPNAPFYHNQSGPLVPLLCNPLDQ-- 433

Query: 442 YYEYQPQNCAANTIRIGDIPKVLKLLTCSDGSNGGCENGQFMSNSEYKTVEAYTNSIQDF 501
              ++P+ CA + + + +  +V K   C   + G C     ++   Y  +    N     
Sbjct: 434 --NHKPRPCAPDEVLLANASQVYKGYICQVNAEGICTTQGRLTQGSYDQMMGAINVAFTL 493

Query: 502 LNVYPGMESLVECQTVKEAFSKILEDHCKPLEKYAYMVWTGLVFVSVVMLCLVLIWTIRA 537
            +  P + S+ +C  V++ F  I   +C  L   +  ++ GL  +S  ++  ++ W I  
Sbjct: 494 DHYGPFLASIADCTFVRDTFRDITTKNCPGLSITSQWIYAGLASLSGAVMFSLIFWLI-- 537