Spg030677 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg030677
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	EXPERA domain-containing protein
Location	scaffold11: 27576124 .. 27579466 (-)
RNA-Seq Expression	Spg030677
Synteny	Spg030677

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACCACAAGGTCTATAAATATGATGATGGGGGCGAGATTTGGTTCTGTCCTGAAGGTCAGTGCGTTCATTTGGTCAAACTTGAAACAGGGGGAAAAGGAAGTCGCCAATGGCGGGTGAATCTGAAGCTGCTCGCCATCCCTACGTTCCCACAGATCTGAAACTCCCCGAATATGTTCCCATTTCACTCCCCCTCTCTACTATACTCGGCGTCTATTTAATCTCTTCTCTCTTCGTCGCTTCGCTCGTCTGGACTCTTTCCGGTATGCTTTCATATTTTACTTCGAATTTTCTTTTCGCTTCCTGGATTTCTTTTTTATGTATTCTGCTTGATTCCTTGCTGGAGAAGGAGATCAATGGTTTGCATAATCTGTTTGTTTGTTATTGCATCGGAGCCCTGTCTTCTTGTTATAGGCCACACGTTGAGTGAGTTAATTGGGAGGAAACCAAATTATTTCAAGATTCTACTCGTTTTTGTATCATCTGAGAATTTTATTGCATTTCGCTTATTGAATGTCTTCGCTGACTTCGAAAGCTGGCGGCGAAACCGATGTAAAAATTCAAGTCTGCATCATTCAATTTTTCTGTTGTTTTTCTTTTCGAGAGTATGAAGATCCATTAAACGGATAGACGCCAGATTTGTTTTGCCTTACCTGAGAGGAAAAGAATGATAGACGTAGCATAGCAAATTGAAATCTATTTGTCCGTTTATCAGTTTCTGTCAATCCACGTTTTGCTTCATTTGGTTTATTGACAAATCCAACTTTCATTTTACACGGCAACGCAGTTTTTCTTTTTTACACGCCACAATTTTCATTTTGACACGAAAATCATTCTTAACCATATATTGGTCTGGTATTCTATCTATTTTAGACTTAAACTCATGATGTTTCCTCTGGTTATACTCCCTTCAAACAGAAGACCATAGTCTTCCTCTAAACAATCATTCATCCACTCAGCTTTCTACCAAATCAGGAACACAACTTTATCCAGATGTTAGCAAAATGCTATTAGGATAAATTGTTAATGTTAAGATTATTATTTGGATATTATTGGCATATTAGCGATTAGCTCGTAAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGTGGATATAAATAAGAGTGGGTTGGGGGAGTGAGAGAGAGGCAGATTTTTTATAGTTTGGAAAGAGTACTGGGGCTCTCGAAGAGCTTGGAAACTTGTAATTTTTGTCTAATATCAATCGAATATTTGCATTAACATGTCCTCCAATTTTTGGTTCCTATCAAATTAGTATTAAAGCTTTTGCACATGGAAATAGATCTTTTAAGTCTAGGAGAGCATTGCTAGACATGTCGTATGTCAGAGAAGTTGTTCAATAACTTAGCATTCTGATGGAGGAGAAAACTAAGGTGCATTTCAGTTACTTTTAGGAAATATAAGAAGATTTGAAGGACGATGAAAGAAATAGAATGCAGAAGCACAAAAATCGTAGCTCAGGTAGAGTGTTGTAGAAACATTGGATCTTAACACCATGAATAAAGTACAAAACACAATGGACAATATATTCAAACATTGTACTGAGGATTCAAAATAGGGATAATCACAATTGCCAACAACAGAAATACCAATTGTGATGCTGCAAACTCATTGGGGAGAAACAAAGGGAGGTAGTTTCTATAGAGTAGTCATGAGAGAGCACCAGGTCTCTCAAAGAGTTTGAGACCTTGTAATTTCTTTTTAATATCAACAGCATATTCTCATTAATTTTCCCTTCAATCTATGGTTTGTATCCGCTCCTGGAAATGGATTTTTAGTATTGGAGAATATTGTTGGAAACATGCAATGTAGCAAAGAAATCGTTGAACGATTTAGCATCCATGAGGGAGAAGAAAATTAAGGACCATTGCAGTAACGTTTCAGAAAAATCAAGAGGTTCATTGTGATTGCAACATTAGGATAAAAAAAGGTTTGATGTTGTGCAGGAAAGCTCAGGTCTGGGCATTTTATTTTTCCTAAAAGAAAGCATACAAGTAGTCACCAATCTTTGTGAACTTAAAACCTCTAGTTGGTGTCGCATTCTTCCTTGTTCCAACCTTCTTGGCTCAGATTATACAACATAGCCATCATCTTAGAATAAAGTTGTAGAAATAGATCTCAAGCACTTGTGTGCAAGCTATGCTATATTTTGCTTTCCCACTGCACCTTGTGTTTCTTTTATTTGGCCTTCCATGTCGTCTGTATACTTGCTCTCTCCATCCTTATCCATTTCTTTTTCATCTAGATTTAGCAATAGACAAAGTTGTAGTTCTTATAGCTGTTCAGAAATGGTTCTATGCATGTTGATTTTATCTTCATTTTCCCCTTATAATAGGGCGACTTCCTAAAAGAGCAAAGATTGAGAGGTTGCTTATGTGCTGGTGGATATTTACGGGCCTCACCCACATGGTTCTGGAAGGTTACTTTGTTTTCTCTCCAGAATTTTACAAGGACACCACTTCTTTCTACCTTGCTGAAGTTTGTAAGTTAATTTAATTCACTTTCTTTATAACCATGTCTGTGGTTGTGGAAGGGCTGTATTATGGATGCTTCTCTGATGTTCATGTGCACAACTTGCCAGGGAAAGAATACAGCAAGGGAGATTCTCGATATGCAGCTAGGGAGTCGGGAATTGTCAGTCTTGAAGCACTGACTTCTGTTTTAGAGGGTCCAGCTTGCATCTTAGCAGCGTAAGATTTTATTATCTGAAATTTAGAATGCTATACATATAAGGAATTACTCTTTAAATTTTTGTTCAGTATTAGATCTAGTATGTGCTTCTATGCTCTTTCTTCCCATTCCTTAACAATTTTGTTGGCCTGGATAATTCACAGATATGCGATAGCTACAGAAAAATCATACAGTTACATACTGCAGTTCGCCATATCATTGGGGCAGCTATATGGGACCTTTATGTACTACATAACCGCTGTGCTGGATGGTGATAATTTTTCTTCCGGCCCATTTTATTACTATTCATATTACGTAGTGGCAAATTCCTTCTGGATTCTTATTCCCTCCCTCATTGCTATGCGGTGTTGGAAGAAAATTTGTGCAGCAATGAACGGTGAAAGCCAAAAGAAAACAAAAGCCCTCTAAGAAGTTCTCAAATGATCGGCTTATTTCATAACATCTATGTTCCTTTAGATTCTGAAATTTGATTAAGAGGCTACACAAAATTCTTGTTGTATTGTACTCTAAGTTATAATTGTGTGACATTCGAAAAACAAGACATTTTGGAATTTGAACATTTAAAAGACCTGCTTTCCCTGTCTAAAATTATGTGCTTACAAGAAGCTTGGTTATGTTATGTTGGTTCTGCA

mRNA sequence

ATGGCGGGTGAATCTGAAGCTGCTCGCCATCCCTACGTTCCCACAGATCTGAAACTCCCCGAATATGTTCCCATTTCACTCCCCCTCTCTACTATACTCGGCGTCTATTTAATCTCTTCTCTCTTCGTCGCTTCGCTCGTCTGGACTCTTTCCGGGCGACTTCCTAAAAGAGCAAAGATTGAGAGGTTGCTTATGTGCTGGTGGATATTTACGGGCCTCACCCACATGGTTCTGGAAGGTTACTTTGTTTTCTCTCCAGAATTTTACAAGGACACCACTTCTTTCTACCTTGCTGAAGTTTGGAAAGAATACAGCAAGGGAGATTCTCGATATGCAGCTAGGGAGTCGGGAATTGTCAGTCTTGAAGCACTGACTTCTGTTTTAGAGGGTCCAGCTTGCATCTTAGCAGCATATGCGATAGCTACAGAAAAATCATACAGTTACATACTGCAGTTCGCCATATCATTGGGGCAGCTATATGGGACCTTTATGTACTACATAACCGCTGTGCTGGATGGTGATAATTTTTCTTCCGGCCCATTTTATTACTATTCATATTACGTAGTGGCAAATTCCTTCTGGATTCTTATTCCCTCCCTCATTGCTATGCGGTGTTGGAAGAAAATTTGTGCAGCAATGAACGGTGAAAGCCAAAAGAAAACAAAAGCCCTCTAA

Coding sequence (CDS)

ATGGCGGGTGAATCTGAAGCTGCTCGCCATCCCTACGTTCCCACAGATCTGAAACTCCCCGAATATGTTCCCATTTCACTCCCCCTCTCTACTATACTCGGCGTCTATTTAATCTCTTCTCTCTTCGTCGCTTCGCTCGTCTGGACTCTTTCCGGGCGACTTCCTAAAAGAGCAAAGATTGAGAGGTTGCTTATGTGCTGGTGGATATTTACGGGCCTCACCCACATGGTTCTGGAAGGTTACTTTGTTTTCTCTCCAGAATTTTACAAGGACACCACTTCTTTCTACCTTGCTGAAGTTTGGAAAGAATACAGCAAGGGAGATTCTCGATATGCAGCTAGGGAGTCGGGAATTGTCAGTCTTGAAGCACTGACTTCTGTTTTAGAGGGTCCAGCTTGCATCTTAGCAGCATATGCGATAGCTACAGAAAAATCATACAGTTACATACTGCAGTTCGCCATATCATTGGGGCAGCTATATGGGACCTTTATGTACTACATAACCGCTGTGCTGGATGGTGATAATTTTTCTTCCGGCCCATTTTATTACTATTCATATTACGTAGTGGCAAATTCCTTCTGGATTCTTATTCCCTCCCTCATTGCTATGCGGTGTTGGAAGAAAATTTGTGCAGCAATGAACGGTGAAAGCCAAAAGAAAACAAAAGCCCTCTAA

Protein sequence

MAGESEAARHPYVPTDLKLPEYVPISLPLSTILGVYLISSLFVASLVWTLSGRLPKRAKIERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDTTSFYLAEVWKEYSKGDSRYAARESGIVSLEALTSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFSSGPFYYYSYYVVANSFWILIPSLIAMRCWKKICAAMNGESQKKTKAL

Homology

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BLAST of Spg030677 vs. NCBI nr
Match: XP_038881035.1 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Benincasa hispida])

HSP 1 Score: 405.6 bits (1041), Expect = 2.8e-109
Identity = 202/222 (90.99%), Postives = 210/222 (94.59%), Query Frame = 0


Query: 1   MAGESEAARHPYVPTDLKLPEYVPISLPLSTILGVYLISSLFVASLVWTLSGRLPKRAKI 60
           MAGESEAARHPY+PTDLKLPEYVPISLP+STIL VYL+SSLFV  LVWTLSGRL KRAKI
Sbjct: 1   MAGESEAARHPYIPTDLKLPEYVPISLPVSTILSVYLLSSLFVTFLVWTLSGRLTKRAKI 60

Query: 61  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDTTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKD TSFYLAEVWKEYSKGDSRYAARESGIVS
Sbjct: 61  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 LEALTSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFSSGP 180
           +E LTSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTF+YYITA+LDGDNFSSGP
Sbjct: 121 IEGLTSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGP 180

Query: 181 FYYYSYYVVANSFWILIPSLIAMRCWKKICAAMNGESQKKTK 223
            YYYSY+VVANSFWILIPSLIA RCWKKIC AM+ ES  KTK
Sbjct: 181 IYYYSYFVVANSFWILIPSLIATRCWKKICIAMSTESHMKTK 222

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BLAST of Spg030677 vs. NCBI nr
Match: XP_022158411.1 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Momordica charantia])

HSP 1 Score: 401.0 bits (1029), Expect = 6.8e-108
Identity = 195/222 (87.84%), Postives = 208/222 (93.69%), Query Frame = 0


Query: 1   MAGESEAARHPYVPTDLKLPEYVPISLPLSTILGVYLISSLFVASLVWTLSGRLPKRAKI 60
           MAG+SEAA HPYVP DL+L EYVP+SLP STI+GVY +SS+ V SLVWTLSGRLPK+AKI
Sbjct: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI 60

Query: 61  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDTTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           ERLLMCWW+FTGLTHM+LEGYFVFSPEFYKD TSFYLAEVWKEYSKGDSRYAARESGIVS
Sbjct: 61  ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 LEALTSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFSSGP 180
           +E LTSVLEGPACILA YAIATEKSYSYILQFAISLGQLYGTF+YYITAVLDGDNFSSGP
Sbjct: 121 IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP 180

Query: 181 FYYYSYYVVANSFWILIPSLIAMRCWKKICAAMNGESQKKTK 223
           FYYY+YYVVANSFWILIPSLIA+RCWKKICAAMN E  KK K
Sbjct: 181 FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRK 222

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BLAST of Spg030677 vs. NCBI nr
Match: XP_004152168.1 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucumis sativus] >KGN52949.1 hypothetical protein Csa_015122 [Cucumis sativus])

HSP 1 Score: 393.7 bits (1010), Expect = 1.1e-105
Identity = 195/224 (87.05%), Postives = 209/224 (93.30%), Query Frame = 0


Query: 1   MAGESEAARHPYVPTDLKLPEYVPISLPLSTILGVYLISSLFVASLVWTLSGRLPKRAKI 60
           MAG SEAA HPYVPTDLKLP+YVPISLPLSTILG YL++SLF+  LVWTLSGRL K+AKI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLTSLFLVLLVWTLSGRLTKKAKI 60

Query: 61  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDTTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           +RLLMCWWIFTGLTHMVLEGYFVFSPEFYKD TSFYLAEVWKEYSKGDSRYAARESGIVS
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 LEALTSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFSSGP 180
           +E LTSVLEGPACILAAYAIA+ KSYSYILQFAISLGQLYGT +YY+TAVLDGD+FSSGP
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYVTAVLDGDDFSSGP 180

Query: 181 FYYYSYYVVANSFWILIPSLIAMRCWKKICAAMNGESQKKTKAL 225
           FYYY+YYVVANSFWILIPSLI +RCWKKIC AM+ ESQ KTK L
Sbjct: 181 FYYYAYYVVANSFWILIPSLIGIRCWKKICIAMSIESQTKTKTL 224

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BLAST of Spg030677 vs. NCBI nr
Match: XP_008454161.1 (PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucumis melo])

HSP 1 Score: 390.6 bits (1002), Expect = 9.2e-105
Identity = 194/224 (86.61%), Postives = 208/224 (92.86%), Query Frame = 0


Query: 1   MAGESEAARHPYVPTDLKLPEYVPISLPLSTILGVYLISSLFVASLVWTLSGRLPKRAKI 60
           MAG SEAA HPYVPTDLKLP+YVPISLPLSTILG YL+SSLF+  L+WTLSGRL K+ KI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLSSLFLVLLLWTLSGRLTKKTKI 60

Query: 61  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDTTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           +RLLMCWWIFTGLTHMVLEGYFVFSPEFYKD TSFYLAEVWKEYSKGDSRYAARESGIV 
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVG 120

Query: 121 LEALTSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFSSGP 180
           +E LTSVLEGPACILAAYAIA+ KSYSYILQFAISLGQLYGT +YYITAVLDGD+FSSGP
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYITAVLDGDDFSSGP 180

Query: 181 FYYYSYYVVANSFWILIPSLIAMRCWKKICAAMNGESQKKTKAL 225
            YYYSYYVVANSFW+LIPSLIA+RCWKKIC AM+ ESQ KTK+L
Sbjct: 181 IYYYSYYVVANSFWLLIPSLIAIRCWKKICNAMSIESQMKTKSL 224

[Close]

BLAST of Spg030677 vs. NCBI nr
Match: XP_022980063.1 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita maxima])

HSP 1 Score: 384.0 bits (985), Expect = 8.7e-103
Identity = 191/222 (86.04%), Postives = 204/222 (91.89%), Query Frame = 0


Query: 1   MAGESEAARHPYVPTDLKLPEYVPISLPLSTILGVYLISSLFVASLVWTLSGRLPKRAKI 60
           MAGESEAARHPY PTDL+LP YVPISLPL+TILGVYL+SSLF+  L+WTLSGRLPK+ KI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDTTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           ERLLMCWWIFTGLTH+VLEGYFVFSPEFYKD  S YLAEVWKEYSKGDSRYAARES IVS
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKD--SSYLAEVWKEYSKGDSRYAARESAIVS 120

Query: 121 LEALTSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFSSGP 180
           LE LT+VLEGPACILA YAIAT+KSYSYILQFAISLGQLYGTFMYYITA+LDGDNFSS P
Sbjct: 121 LEGLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSP 180

Query: 181 FYYYSYYVVANSFWILIPSLIAMRCWKKICAAMNGESQKKTK 223
            YYY YYV+ NSFWILIPSLIA+RCWKKIC AM+ ES KKTK
Sbjct: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTK 220

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BLAST of Spg030677 vs. ExPASy Swiss-Prot
Match: O48962 (Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Arabidopsis thaliana OX=3702 GN=At1g20050 PE=2 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 1.8e-79
Identity = 145/220 (65.91%), Postives = 177/220 (80.45%), Query Frame = 0


Query: 6   EAARHPYVPTDLKLPEYVPISLPLSTILGVYLISSLFVASLVWTLSGRLPKRAKIERLLM 65
           E   HPYVP DL LP YVPIS+ +S+I+ +YL SSL V SLVW L GR  K+AK+++LLM
Sbjct: 2   EELAHPYVPRDLNLPGYVPISMSMSSIVSIYLGSSLLVVSLVWLLFGR--KKAKLDKLLM 61

Query: 66  CWWIFTGLTHMVLEGYFVFSPEFYKDTTSFYLAEVWKEYSKGDSRYAARESGIVSLEALT 125
           CWW FTGLTH++LEGYFVFSPEF+KD TS YLAEVWKEYSKGDSRY  R+S +VS+E +T
Sbjct: 62  CWWTFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGDSRYVGRDSAVVSVEGIT 121

Query: 126 SVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFSSGPFYYYS 185
           +V+ GPA +LA YAIA EKSYSY+LQ AIS+ QLYG  +Y+ITA+L+GDNF++  FYYYS
Sbjct: 122 AVIVGPASLLAIYAIAKEKSYSYVLQLAISVCQLYGCLVYFITAILEGDNFATNSFYYYS 181

Query: 186 YYVVANSFWILIPSLIAMRCWKKICAA---MNGESQKKTK 223
           YY+ AN +W+LIPSLI+ RCWKKICAA    N   + KTK
Sbjct: 182 YYIGANCWWVLIPSLISFRCWKKICAAAAIANNNVETKTK 219

[Close]

BLAST of Spg030677 vs. ExPASy Swiss-Prot
Match: Q9FTZ2 (Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0103600 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 7.4e-65
Identity = 119/211 (56.40%), Postives = 157/211 (74.41%), Query Frame = 0


Query: 10  HPYVPTDLKLPEYVPISLPLSTILGVYLISSLFVASLVWTLSGRLPKR-AKIERLLMCWW 69
           HPY P +L LP +VP+ L  + IL  YL +SLF+   VW +SGR  +R +  +R LMCWW
Sbjct: 7   HPYAPAELHLPGFVPLQLSQAQILVPYLATSLFLLLAVWLISGRCSRRLSDTDRWLMCWW 66

Query: 70  IFTGLTHMVLEGYFVFSPEFYKDTTSFYLAEVWKEYSKGDSRYAARESGIVSLEALTSVL 129
            FTGLTH+++EG FVF+P F+ +    Y  EVWKEYSKGDSRY AR+   V++E +T+VL
Sbjct: 67  AFTGLTHIIIEGTFVFAPNFFSNQNPSYFDEVWKEYSKGDSRYVARDPATVTVEGITAVL 126

Query: 130 EGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFSSGPFYYYSYYV 189
           EGPA +LA YAIA+ KSYS+ILQF + LGQLYG  +Y+ITA LDG NF + PFY+++Y++
Sbjct: 127 EGPASLLAVYAIASGKSYSHILQFTVCLGQLYGCLVYFITAYLDGFNFWTSPFYFWAYFI 186

Query: 190 VANSFWILIPSLIAMRCWKKICAAMNGESQK 220
            ANS W++IP++IA+R WKKICAA  GE  K
Sbjct: 187 GANSSWVVIPTMIAIRSWKKICAAFQGEKVK 217

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BLAST of Spg030677 vs. ExPASy Swiss-Prot
Match: Q9JJ46 (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Rattus norvegicus OX=10116 GN=Ebp PE=1 SV=3)

HSP 1 Score: 127.1 bits (318), Expect = 2.5e-28
Identity = 75/220 (34.09%), Postives = 121/220 (55.00%), Query Frame = 0


Query: 10  HPYVPTDLKLPEYVPISLPLSTIL-GVYLISSLFVASLVWTLSGRLP--KRAKIERLLMC 69
           HPY P  L+L  +VP  LP   IL G++  S + +  + W LS R+         RL +C
Sbjct: 9   HPYWPRHLRLDNFVPNDLPTWHILVGLFSFSGVLIV-ITWLLSSRVSVVPLGTGRRLALC 68

Query: 70  WWIFTGLTHMVLEGYFVFSPEFYKDTTSFYLAEVWKEYSKGDSRYAARESGIVSLEALTS 129
           W+      H+V+EG+F F  E   +  +F L+++WKEYSKGDSRY   +  IV +E++T+
Sbjct: 69  WFAVCTFIHLVIEGWFSFYHEILLEDQAF-LSQLWKEYSKGDSRYILSDGFIVCMESVTA 128

Query: 130 VLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFSSG----PFY 189
            L GP  +    A    + + ++LQ  +S+GQ+YG  +Y++T + DG  F  G    P Y
Sbjct: 129 CLWGPLSLWVVIAFLRHQPFRFVLQLVVSVGQIYGDVLYFLTELRDG--FQHGELGHPLY 188

Query: 190 YYSYYVVANSFWILIPSLIAMRCWKKICAAMNGESQKKTK 223
           ++ Y+V+ N+ W++IP ++     K +  A +    K  K
Sbjct: 189 FWFYFVIMNAIWLVIPGILVFDAIKHLTNAQSMLDNKVMK 224

[Close]

BLAST of Spg030677 vs. ExPASy Swiss-Prot
Match: P70245 (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Mus musculus OX=10090 GN=Ebp PE=1 SV=3)

HSP 1 Score: 126.3 bits (316), Expect = 4.3e-28
Identity = 76/219 (34.70%), Postives = 123/219 (56.16%), Query Frame = 0


Query: 10  HPYVPTDLKLPEYVPISLPLSTIL-GVYLISSLFVASLVWTLSGR---LPKRAKIERLLM 69
           HPY P  LKL  +VP  LP S IL G++ IS   +  + W LS R   +P  A   RL +
Sbjct: 9   HPYWPRHLKLDNFVPNDLPTSHILVGLFSISGGLIV-ITWLLSSRASVVPLGAG-RRLAL 68

Query: 70  CWWIFTGLTHMVLEGYFVFSPEFYKDTTSFYLAEVWKEYSKGDSRYAARESGIVSLEALT 129
           CW+      H+V+EG+F        +  +F L+++WKEYSKGDSRY   +S +V +E +T
Sbjct: 69  CWFAVCTFIHLVIEGWFSLYNGILLEDQAF-LSQLWKEYSKGDSRYILSDSFVVCMETVT 128

Query: 130 SVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFS--SGPFYY 189
           + L GP  +    A   ++ + ++LQ  +S+GQ+YG  +Y++T + +G        P Y+
Sbjct: 129 ACLWGPLSLWVVIAFLRQQPFRFVLQLVVSMGQIYGDVLYFLTELHEGLQHGEIGHPVYF 188

Query: 190 YSYYVVANSFWILIPSLIAMRCWKKICAAMNGESQKKTK 223
           + Y+V  N+ W++IPS++ +   K + +A +    K  K
Sbjct: 189 WFYFVFLNAVWLVIPSILVLDAIKHLTSAQSVLDSKVMK 224

[Close]

BLAST of Spg030677 vs. ExPASy Swiss-Prot
Match: Q15125 (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Homo sapiens OX=9606 GN=EBP PE=1 SV=3)

HSP 1 Score: 124.4 bits (311), Expect = 1.6e-27
Identity = 76/223 (34.08%), Postives = 123/223 (55.16%), Query Frame = 0


Query: 10  HPYVPTDLKLPEYVPISLPLSTIL-GVYLISSLFVASLVWTLSGR--LPKRAKIERLLMC 69
           HPY P  L+L  +VP   P   IL G++ ++ + V +  W LSGR  +       RL +C
Sbjct: 9   HPYWPQHLRLDNFVPNDRPTWHILAGLFSVTGVLVVT-TWLLSGRAAVVPLGTWRRLSLC 68

Query: 70  WWIFTGLTHMVLEGYFVFSPEFYKDT--TSFYLAEVWKEYSKGDSRYAARESGIVSLEAL 129
           W+   G  H+V+EG+FV    +Y+D      +L+++WKEY+KGDSRY   ++  V +E +
Sbjct: 69  WFAVCGFIHLVIEGWFVL---YYEDLLGDQAFLSQLWKEYAKGDSRYILGDNFTVCMETI 128

Query: 130 TSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFSSG----P 189
           T+ L GP  +    A   +    +ILQ  +S+GQ+YG  +Y++T   DG  F  G    P
Sbjct: 129 TACLWGPLSLWVVIAFLRQHPLRFILQLVVSVGQIYGDVLYFLTEHRDG--FQHGELGHP 188

Query: 190 FYYYSYYVVANSFWILIPSLIAMRCWKKICAAMNGESQKKTKA 224
            Y++ Y+V  N+ W+++P ++ +   K +  A +    K TKA
Sbjct: 189 LYFWFYFVFMNALWLVLPGVLVLDAVKHLTHAQSTLDAKATKA 225

[Close]

BLAST of Spg030677 vs. ExPASy TrEMBL
Match: A0A6J1E0U0 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Momordica charantia OX=3673 GN=LOC111024900 PE=3 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 3.3e-108
Identity = 195/222 (87.84%), Postives = 208/222 (93.69%), Query Frame = 0


Query: 1   MAGESEAARHPYVPTDLKLPEYVPISLPLSTILGVYLISSLFVASLVWTLSGRLPKRAKI 60
           MAG+SEAA HPYVP DL+L EYVP+SLP STI+GVY +SS+ V SLVWTLSGRLPK+AKI
Sbjct: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI 60

Query: 61  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDTTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           ERLLMCWW+FTGLTHM+LEGYFVFSPEFYKD TSFYLAEVWKEYSKGDSRYAARESGIVS
Sbjct: 61  ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 LEALTSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFSSGP 180
           +E LTSVLEGPACILA YAIATEKSYSYILQFAISLGQLYGTF+YYITAVLDGDNFSSGP
Sbjct: 121 IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP 180

Query: 181 FYYYSYYVVANSFWILIPSLIAMRCWKKICAAMNGESQKKTK 223
           FYYY+YYVVANSFWILIPSLIA+RCWKKICAAMN E  KK K
Sbjct: 181 FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRK 222

[Close]

BLAST of Spg030677 vs. ExPASy TrEMBL
Match: A0A0A0KVP4 (EXPERA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G007050 PE=3 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 5.3e-106
Identity = 195/224 (87.05%), Postives = 209/224 (93.30%), Query Frame = 0


Query: 1   MAGESEAARHPYVPTDLKLPEYVPISLPLSTILGVYLISSLFVASLVWTLSGRLPKRAKI 60
           MAG SEAA HPYVPTDLKLP+YVPISLPLSTILG YL++SLF+  LVWTLSGRL K+AKI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLTSLFLVLLVWTLSGRLTKKAKI 60

Query: 61  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDTTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           +RLLMCWWIFTGLTHMVLEGYFVFSPEFYKD TSFYLAEVWKEYSKGDSRYAARESGIVS
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 LEALTSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFSSGP 180
           +E LTSVLEGPACILAAYAIA+ KSYSYILQFAISLGQLYGT +YY+TAVLDGD+FSSGP
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYVTAVLDGDDFSSGP 180

Query: 181 FYYYSYYVVANSFWILIPSLIAMRCWKKICAAMNGESQKKTKAL 225
           FYYY+YYVVANSFWILIPSLI +RCWKKIC AM+ ESQ KTK L
Sbjct: 181 FYYYAYYVVANSFWILIPSLIGIRCWKKICIAMSIESQTKTKTL 224

[Close]

BLAST of Spg030677 vs. ExPASy TrEMBL
Match: A0A1S3BY31 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucumis melo OX=3656 GN=LOC103494647 PE=3 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.5e-105
Identity = 194/224 (86.61%), Postives = 208/224 (92.86%), Query Frame = 0


Query: 1   MAGESEAARHPYVPTDLKLPEYVPISLPLSTILGVYLISSLFVASLVWTLSGRLPKRAKI 60
           MAG SEAA HPYVPTDLKLP+YVPISLPLSTILG YL+SSLF+  L+WTLSGRL K+ KI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLSSLFLVLLLWTLSGRLTKKTKI 60

Query: 61  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDTTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           +RLLMCWWIFTGLTHMVLEGYFVFSPEFYKD TSFYLAEVWKEYSKGDSRYAARESGIV 
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVG 120

Query: 121 LEALTSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFSSGP 180
           +E LTSVLEGPACILAAYAIA+ KSYSYILQFAISLGQLYGT +YYITAVLDGD+FSSGP
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYITAVLDGDDFSSGP 180

Query: 181 FYYYSYYVVANSFWILIPSLIAMRCWKKICAAMNGESQKKTKAL 225
            YYYSYYVVANSFW+LIPSLIA+RCWKKIC AM+ ESQ KTK+L
Sbjct: 181 IYYYSYYVVANSFWLLIPSLIAIRCWKKICNAMSIESQMKTKSL 224

[Close]

BLAST of Spg030677 vs. ExPASy TrEMBL
Match: A0A6J1IY56 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucurbita maxima OX=3661 GN=LOC111479580 PE=3 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 4.2e-103
Identity = 191/222 (86.04%), Postives = 204/222 (91.89%), Query Frame = 0


Query: 1   MAGESEAARHPYVPTDLKLPEYVPISLPLSTILGVYLISSLFVASLVWTLSGRLPKRAKI 60
           MAGESEAARHPY PTDL+LP YVPISLPL+TILGVYL+SSLF+  L+WTLSGRLPK+ KI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDTTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           ERLLMCWWIFTGLTH+VLEGYFVFSPEFYKD  S YLAEVWKEYSKGDSRYAARES IVS
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKD--SSYLAEVWKEYSKGDSRYAARESAIVS 120

Query: 121 LEALTSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFSSGP 180
           LE LT+VLEGPACILA YAIAT+KSYSYILQFAISLGQLYGTFMYYITA+LDGDNFSS P
Sbjct: 121 LEGLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSP 180

Query: 181 FYYYSYYVVANSFWILIPSLIAMRCWKKICAAMNGESQKKTK 223
            YYY YYV+ NSFWILIPSLIA+RCWKKIC AM+ ES KKTK
Sbjct: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTK 220

[Close]

BLAST of Spg030677 vs. ExPASy TrEMBL
Match: A0A6J1GU98 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucurbita moschata OX=3662 GN=LOC111457598 PE=3 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.3e-101
Identity = 186/222 (83.78%), Postives = 203/222 (91.44%), Query Frame = 0


Query: 1   MAGESEAARHPYVPTDLKLPEYVPISLPLSTILGVYLISSLFVASLVWTLSGRLPKRAKI 60
           MAGESEAARHPY PTDL+LP YVPISLPL+TILGVY++SSLF+  L+WTLSGRLPK+ KI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDTTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           ERLLMCWWIFTGLTH+VLEGYFVFSPEFYKD +  YLAEVWKEYSKGDSRYA R+S IVS
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDGS--YLAEVWKEYSKGDSRYATRDSAIVS 120

Query: 121 LEALTSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFSSGP 180
           LE LT+VLEGPACILA YAIAT+KSYSY+LQFAISLGQLYGTFMYYITA+LDGDNFSS P
Sbjct: 121 LEGLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSP 180

Query: 181 FYYYSYYVVANSFWILIPSLIAMRCWKKICAAMNGESQKKTK 223
            YYY YYV+ NSFWILIPSLIA+RCWKKIC AM+ ES KKTK
Sbjct: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTK 220

[Close]

BLAST of Spg030677 vs. TAIR 10
Match: AT1G20050.1 (C-8,7 sterol isomerase )

HSP 1 Score: 297.0 bits (759), Expect = 1.3e-80
Identity = 145/220 (65.91%), Postives = 177/220 (80.45%), Query Frame = 0


Query: 6   EAARHPYVPTDLKLPEYVPISLPLSTILGVYLISSLFVASLVWTLSGRLPKRAKIERLLM 65
           E   HPYVP DL LP YVPIS+ +S+I+ +YL SSL V SLVW L GR  K+AK+++LLM
Sbjct: 2   EELAHPYVPRDLNLPGYVPISMSMSSIVSIYLGSSLLVVSLVWLLFGR--KKAKLDKLLM 61

Query: 66  CWWIFTGLTHMVLEGYFVFSPEFYKDTTSFYLAEVWKEYSKGDSRYAARESGIVSLEALT 125
           CWW FTGLTH++LEGYFVFSPEF+KD TS YLAEVWKEYSKGDSRY  R+S +VS+E +T
Sbjct: 62  CWWTFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGDSRYVGRDSAVVSVEGIT 121

Query: 126 SVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFMYYITAVLDGDNFSSGPFYYYS 185
           +V+ GPA +LA YAIA EKSYSY+LQ AIS+ QLYG  +Y+ITA+L+GDNF++  FYYYS
Sbjct: 122 AVIVGPASLLAIYAIAKEKSYSYVLQLAISVCQLYGCLVYFITAILEGDNFATNSFYYYS 181

Query: 186 YYVVANSFWILIPSLIAMRCWKKICAA---MNGESQKKTK 223
           YY+ AN +W+LIPSLI+ RCWKKICAA    N   + KTK
Sbjct: 182 YYIGANCWWVLIPSLISFRCWKKICAAAAIANNNVETKTK 219

The following BLAST results are available for this feature:

BLAST of Spg030677 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881035.1	2.8e-109	90.99	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Benincasa hispida]	[more]
XP_022158411.1	6.8e-108	87.84	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Momordica charantia]	[more]
XP_004152168.1	1.1e-105	87.05	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucumis sativus] >KG...	[more]
XP_008454161.1	9.2e-105	86.61	PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucumis m...	[more]
XP_022980063.1	8.7e-103	86.04	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita maxima]	[more]

BLAST of Spg030677 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48962	1.8e-79	65.91	Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Arabidopsis thalia...	[more]
Q9FTZ2	7.4e-65	56.40	Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Oryza sativa subsp...	[more]
Q9JJ46	2.5e-28	34.09	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Rattus norvegicus OX=10116 ...	[more]
P70245	4.3e-28	34.70	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Mus musculus OX=10090 GN=Eb...	[more]
Q15125	1.6e-27	34.08	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Homo sapiens OX=9606 GN=EBP...	[more]

BLAST of Spg030677 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E0U0	3.3e-108	87.84	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Momordica charanti...	[more]
A0A0A0KVP4	5.3e-106	87.05	EXPERA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G007050 PE=3...	[more]
A0A1S3BY31	4.5e-105	86.61	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucumis melo OX=36...	[more]
A0A6J1IY56	4.2e-103	86.04	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucurbita maxima O...	[more]
A0A6J1GU98	1.3e-101	83.78	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucurbita moschata...	[more]

BLAST of Spg030677 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20050.1	1.3e-80	65.91	C-8,7 sterol isomerase	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF05241	EBP	coord: 97..205 e-value: 3.6E-18 score: 66.0
None	No IPR available	PANTHER	PTHR14207:SF7	BNAANNG14740D PROTEIN	coord: 6..222
IPR007905	Emopamil-binding protein	PANTHER	PTHR14207	STEROL ISOMERASE	coord: 6..222
IPR033118	EXPERA domain	PROSITE	PS51751	EXPERA	coord: 60..202 score: 14.946956

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg030677.1	Spg030677.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
biological_process	GO:0016126	sterol biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0000247	C-8 sterol isomerase activity
molecular_function	GO:0047750	cholestenol delta-isomerase activity
molecular_function	GO:0004769	steroid delta-isomerase activity