Spg030603 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg030603
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionC2 domain-containing protein
Locationscaffold6: 31816192 .. 31820463 (+)
RNA-Seq ExpressionSpg030603
SyntenySpg030603
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACTCAGATGTAGCTTGGTTCGAGGAATCGAGTCGCGGAGGAGTTGCTTGGGTCATTCGTGACTCCGATGGATCCTCGATCTGTTTCGGGATGAAGCATATCAAAAGAAGATGGGATATCAATTCGCTTGAAGCGCTTGCTATTTGGGAAGGGTTAAAATGCCTTTCTTACTTCAGGAAGGAGGAGCACTTACCTCTGATTATCGAGTCGGACGCCTTAGAGGTCGTCAGCTGCCTCAATGATCAATCTCAATTTCTCTCGAAGACGAAGAACATCATCGATGCTATTCGCTCCATCTGCTCCGATTGGGGCTCTGTGGAAGTTGTGCACTGCCCTAGGGTAGAAAATCGAGTCGCCCACTCCATTGCTCGCCTTGTTGCTGAGCTTTGTTTTGATTCGCCTGTGGATTTTTCCCAATCTTGGGAAGATGAGTTGCTTTTTTGGTTGTCTAGCTTTCCCAATTGGTTTCTTTCCCTCCTTTATGAGGCTAGTTGTAACCGTTCTCTTTGATTAGTGAAGGTCTTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGATCCGTTCTCTTTTAACCTTCTATTCAAGGTCAATTTGGAATACATTTTAATCTAAGGATCTCTTTGGAATACATTTTAATTTAAATGTTTTTCAAGAATGCATTTTTTAAAAAACATTTTGTTTAAAAATTATTTAGAATATTTAAACACTCATATGTTTGGCTACATCTCCTCAAATGTTTTTATACACAACTTAGGGTGTATTTGGTTTAACTTTTCAAGTGTTTAATTTTGAAAAAAGTTATAGTGTTTGGCAACCACTAAAAATGACTTATTAGAAAACAAGATTGATAAATAAACCATTTTGAGAGAAACACTTGAAACCAACCTCTTTAAAAAGAGTTTTTAAGTATACAATAACTTGCATATAAAAACACTTATGAGGAAATGTAACCAAACATATGAGTGTTTAAATTCTAAACAATTTTTATACAAAATGTTTTTTAAAAAATGCATTCTTGAAAAACATTTAAATTAAAATGTATTCCAAACGCATCCTTATTGTATACCTAAAAAGTTTTTAAAAAAGTTGGTTTCAAGTGTTTCTCTCAAAATGGTTTATTTATGAATCTCATTTTCTAACAAGTCATTTTTAGTGGTTGCCAAACACTAGAAATTTTTTCTTTTTATCAAAATTAAACACTTGAAAAGTTACACCGAACACACCCTAAATGTTTTTTAAGAATGCATTTTTTAAAAAACATTTTGTATAAAAATTGTTTAGAATTTAAACACTCATATGTTTGGTTGCATTTCCTCATAATGTTTTTATACATAAGTTATTGTATACTTAAATACTTTTTTAAAAAATTGGTTTGAAGTGTTTCTCTCAAAATGGTTTATTTATCAATCTCATTTTCTAATAAGTCATTTTTAGTGGTTGCCAAAAACTAGAATTTTTTTCAAAATGATTTTTTTTTTCAAAATTAAACACTTAAAAAGTTAAACTAAACACACCCTTAATTCAAATACTCAAACAATGCCAAATGTCTTTCCATCACTCTTATGTTGACCACTCACATTCTACATGTGATATGTTGATGCATTTTAAACTATTAACACTATGCTTGATTCTCAGCAGCATCCTTCGAGAATACTTGCACATTAACTTTCTCCTGCGAGTTCCTTAGGGTAATTTTTAAACTCCACTACCTTCTTCTCTTCTGAATTACTTGCATCAAATAGCATATTTCCAACATTTAAAAAAGTTCATCCATATATTTCCTTTGCCAAAGCCCACAGAAAAAGATGGAACTTCCACTTGTTTTCTCTCTCACCAAACCCTAAACTAAACACTAAAACAGTATCTCCAAGCAATTCCTTCCAACACCCTTTTTCTGTTATCATATTTTAAAAACATTTCTTCATCAATAATTGAACAAAGCTAGCGATATTGATCATTCAAAAGCTGAGAGCAGCACTCAGAAAACAGTAATCAAAACACACTGCAGATGAGAACAGAGTGACCACTGAAACTGCGTTTTTTATGGGAATAAAAAGTCATCTGTTTTTTATTTATGTCCCACTCCCGAAAACCCCACCATTCATATTCGGTCTCATTGGTCAAATCTGAGCCCCACGCGTGGGACTTTGTATTAAAAAGCCCCCATTCCTCTGGATCCTAAAACCCCATTTCAAAAATTCCCTAAAAAATTATCCTAAACTTGCACAGCTTCCAATTCCAATTCAATTCCCACTCTCGAGCCGATTTCGAGTTCTCAAGTGCACCAGATTTTCCGGATTATCATTTTTGACTTTGGAGCCTAAAGGCAACAATACAAACAAACAGAGCCGTTCTTTGTTTTGCTTTCTTTTGCCAATTTCAGCTTCTCTTTTTCTTCTTTTGTCTTTTCGCCTTGGGATTCTACGATATTCAATTTGGTTCGTATATTCTTTGTAGAACTGTGTTTCTGGCTGTGGGTTTTTTTTTTCACATTCTGCTGCTCATTTCTGGTATTTGGTTTTAGGGTTTTTCATGTTTTTCAGTTTTAGATTGTGGGGTTTTGTCAGTTTTGAGGTTTTTGCTTGTGGGTGTTCAAAATTTTCTGTTTATTCTTTGATTTGGGGCTTTTTTGTTTGGTTTCTGTCTTTTTTGCTCTGTTTCTTTGTATTTGGTGTGATAAACTCTGCTGATTCCAGTGTTAAAGTTGTGGGTTGTTTAGAAAATTTCAAAGTTGAATGGGGAACTTGAAATTTATCTAATGAGAAGTGTTACTTTCTTTGTTTGAAATTGATTTCTTTTGGATTTTGCAGGTGTTTTTAATGGCTTCTCCAGTTAAGAACTTGGCTGTGTCAGAGGAACAGAAGAAGGAATGTACTGTGTCAAATGGGAAGGAAAGTGAGGAAGTTGTTGGTGTTCTTGAGGTGTTTGTTCACCAAGCAAGAGACATTCATAACATATGTATTTACCACAAGCAAGATGTGTATGCTAGGCTTTCTTTTACAACTGATCCTAAATATTCTGTCGCCACAAAGACCATCAATGGTGGTGGGAGGAATCCTGTCTTCAATGAGAGTGTTCGGCTCGACGTTCGATCGATCGATACGTTGCTTAAATGCGAGATTTGGATGCTGAGCAGGGTCAAGAATTATCTTGAGGATCAGTTGCTTGGTTTTGCTTTGATCCCTCTAACAGAAGTTGTTGTTGTCAATGGGAAGTTAGAGAAAGAATTCTCTCTTTCGTCGACCGATTTGTTCCATTCTCCTGCCGGGTTCGTGCAGCTATCGCTTTCGTATAATGGAGCTTCTCCAGAGGTCATGGCGATCCCGGTCATGACTGCTGCTCCACTCGAAACAATTGCAGCGGTAGAGGATTCGAATCTAATCAAGTCACATCCAAGTGATTTGGACATGATCGAATTCCCCGATCCGAAGATTGAAAATGAAGATCAGATAATGGTCTCCGAGTATTTCGGTATCTCGTGTTCGAATTTGGATTCCGAATCTTCTGAGAGCTTGGCCATTTCTGAGGGTGAGAATCATGGTATTTCTGCTGTCGAAAGCTATTCGATAACGAACGAGAAGTCGAGTGAAGATTCGAAGATCGATTCTCCACCTAGCAGTGTGCAAAATGATGCAGTTTCTTCACCAAGCTCGGAGTCGTCAGAAGCTCCAGCCAGTTCAAAATCTACAACTCAAGAACATGTTTCAGCACCAAATGCCACAAGTGAGAAAGAACAAAATGCTGAAAATGGTGAGGTTAATGGTTCCTCTAGTGAGGCAGCAATTGACACAATTACAAAGCCAGTAGTTTCTGTGAGCATTGAGCCAGAGGACAAGGTGGTGCAACAAGACATTGTGGACATGTACATGAAAAGTATGCAACAATTCACAGAGTCATTGGCAAAGATGAAGCTTCCTTTGGATATTGATACTAATGGCCCTCCCAGTTCCGGTAGTTCGAGCTCCGACCCGAAGTTACAATCGCCGAAAAACAGCGGTTCTCGTGTGTTTTACGGGAGTAGGGCGTTCTTCTGAGCGGATTTGTTGCCTGTGGTGAAAAGTCAGGTGACATTAGATTTTAGGATGATGAGACTAATATCAGTAAAAGGATGATGCTTGTAGTAAGTGTTTTTCTTTTCTTTCTTCCATTATTGGTTTTGAGATGTTTTTGATGAAAGAATTTTTAAAACTTTGTCACTTGTAGCTTTGTTCCATCAATGTTGGTAATGGTTGTTAGTTAGTATGATTTGAATTTTGATATGTTTCCC

mRNA sequence

ATGAACTCAGATGTAGCTTGGTTCGAGGAATCGAGTCGCGGAGGAGTTGCTTGGGTCATTCGTGACTCCGATGGATCCTCGATCTGTTTCGGGATGAAGCATATCAAAAGAAGATGGGATATCAATTCGCTTGAAGCGCTTGCTATTTGGGAAGGGTTAAAATGCCTTTCTTACTTCAGGAAGGAGGAGCACTTACCTCTGATTATCGAGTCGGACGCCTTAGAGGTCGTCAGCTGCCTCAATGATCAATCTCAATTTCTCTCGAAGACGAAGAACATCATCGATGCTATTCGCTCCATCTGCTCCGATTGGGGCTCTGTGGAAGTTGTGCACTGCCCTAGGGTAGAAAATCGAGTCGCCCACTCCATTGCTCGCCTTGTTGCTGAGCTTTCAGCATCCTTCGAGAATACTTGCACATTAACTTTCTCCTGCGAGTTCCTTAGGCTTCTCTTTTTCTTCTTTTGTCTTTTCGCCTTGGGATTCTACGATATTCAATTTGGTTCGTATATTCTTTGTAGAACTGTGTTTCTGGCTGTGTTTTTAATGGCTTCTCCAGTTAAGAACTTGGCTGTGTCAGAGGAACAGAAGAAGGAATGTACTGTGTCAAATGGGAAGGAAAGTGAGGAAGTTGTTGGTGTTCTTGAGGTGTTTGTTCACCAAGCAAGAGACATTCATAACATATGTATTTACCACAAGCAAGATGTGTATGCTAGGCTTTCTTTTACAACTGATCCTAAATATTCTGTCGCCACAAAGACCATCAATGGTGGTGGGAGGAATCCTGTCTTCAATGAGAGTGTTCGGCTCGACGTTCGATCGATCGATACGTTGCTTAAATGCGAGATTTGGATGCTGAGCAGGGTCAAGAATTATCTTGAGGATCAGTTGCTTGGTTTTGCTTTGATCCCTCTAACAGAAGTTGTTGTTGTCAATGGGAAGTTAGAGAAAGAATTCTCTCTTTCGTCGACCGATTTGTTCCATTCTCCTGCCGGGTTCGTGCAGCTATCGCTTTCGTATAATGGAGCTTCTCCAGAGGTCATGGCGATCCCGGTCATGACTGCTGCTCCACTCGAAACAATTGCAGCGGTAGAGGATTCGAATCTAATCAAGTCACATCCAAGTGATTTGGACATGATCGAATTCCCCGATCCGAAGATTGAAAATGAAGATCAGATAATGGTCTCCGAGTATTTCGGTATCTCGTGTTCGAATTTGGATTCCGAATCTTCTGAGAGCTTGGCCATTTCTGAGGGTGAGAATCATGGTATTTCTGCTGTCGAAAGCTATTCGATAACGAACGAGAAGTCGAGTGAAGATTCGAAGATCGATTCTCCACCTAGCAGTGTGCAAAATGATGCAGTTTCTTCACCAAGCTCGGAGTCGTCAGAAGCTCCAGCCAGTTCAAAATCTACAACTCAAGAACATGTTTCAGCACCAAATGCCACAAGTGAGAAAGAACAAAATGCTGAAAATGGTGAGGTTAATGGTTCCTCTAGTGAGGCAGCAATTGACACAATTACAAAGCCAGTAGTTTCTGTGAGCATTGAGCCAGAGGACAAGGTGGTGCAACAAGACATTGTGGACATGTACATGAAAAGTATGCAACAATTCACAGAGTCATTGGCAAAGATGAAGCTTCCTTTGGATATTGATACTAATGGCCCTCCCAGTTCCGGTAGTTCGAGCTCCGACCCGAAGTTACAATCGCCGAAAAACAGCGGTTCTCGTGTGTTTTACGGGAGTAGGGCGTTCTTCTGA

Coding sequence (CDS)

ATGAACTCAGATGTAGCTTGGTTCGAGGAATCGAGTCGCGGAGGAGTTGCTTGGGTCATTCGTGACTCCGATGGATCCTCGATCTGTTTCGGGATGAAGCATATCAAAAGAAGATGGGATATCAATTCGCTTGAAGCGCTTGCTATTTGGGAAGGGTTAAAATGCCTTTCTTACTTCAGGAAGGAGGAGCACTTACCTCTGATTATCGAGTCGGACGCCTTAGAGGTCGTCAGCTGCCTCAATGATCAATCTCAATTTCTCTCGAAGACGAAGAACATCATCGATGCTATTCGCTCCATCTGCTCCGATTGGGGCTCTGTGGAAGTTGTGCACTGCCCTAGGGTAGAAAATCGAGTCGCCCACTCCATTGCTCGCCTTGTTGCTGAGCTTTCAGCATCCTTCGAGAATACTTGCACATTAACTTTCTCCTGCGAGTTCCTTAGGCTTCTCTTTTTCTTCTTTTGTCTTTTCGCCTTGGGATTCTACGATATTCAATTTGGTTCGTATATTCTTTGTAGAACTGTGTTTCTGGCTGTGTTTTTAATGGCTTCTCCAGTTAAGAACTTGGCTGTGTCAGAGGAACAGAAGAAGGAATGTACTGTGTCAAATGGGAAGGAAAGTGAGGAAGTTGTTGGTGTTCTTGAGGTGTTTGTTCACCAAGCAAGAGACATTCATAACATATGTATTTACCACAAGCAAGATGTGTATGCTAGGCTTTCTTTTACAACTGATCCTAAATATTCTGTCGCCACAAAGACCATCAATGGTGGTGGGAGGAATCCTGTCTTCAATGAGAGTGTTCGGCTCGACGTTCGATCGATCGATACGTTGCTTAAATGCGAGATTTGGATGCTGAGCAGGGTCAAGAATTATCTTGAGGATCAGTTGCTTGGTTTTGCTTTGATCCCTCTAACAGAAGTTGTTGTTGTCAATGGGAAGTTAGAGAAAGAATTCTCTCTTTCGTCGACCGATTTGTTCCATTCTCCTGCCGGGTTCGTGCAGCTATCGCTTTCGTATAATGGAGCTTCTCCAGAGGTCATGGCGATCCCGGTCATGACTGCTGCTCCACTCGAAACAATTGCAGCGGTAGAGGATTCGAATCTAATCAAGTCACATCCAAGTGATTTGGACATGATCGAATTCCCCGATCCGAAGATTGAAAATGAAGATCAGATAATGGTCTCCGAGTATTTCGGTATCTCGTGTTCGAATTTGGATTCCGAATCTTCTGAGAGCTTGGCCATTTCTGAGGGTGAGAATCATGGTATTTCTGCTGTCGAAAGCTATTCGATAACGAACGAGAAGTCGAGTGAAGATTCGAAGATCGATTCTCCACCTAGCAGTGTGCAAAATGATGCAGTTTCTTCACCAAGCTCGGAGTCGTCAGAAGCTCCAGCCAGTTCAAAATCTACAACTCAAGAACATGTTTCAGCACCAAATGCCACAAGTGAGAAAGAACAAAATGCTGAAAATGGTGAGGTTAATGGTTCCTCTAGTGAGGCAGCAATTGACACAATTACAAAGCCAGTAGTTTCTGTGAGCATTGAGCCAGAGGACAAGGTGGTGCAACAAGACATTGTGGACATGTACATGAAAAGTATGCAACAATTCACAGAGTCATTGGCAAAGATGAAGCTTCCTTTGGATATTGATACTAATGGCCCTCCCAGTTCCGGTAGTTCGAGCTCCGACCCGAAGTTACAATCGCCGAAAAACAGCGGTTCTCGTGTGTTTTACGGGAGTAGGGCGTTCTTCTGA

Protein sequence

MNSDVAWFEESSRGGVAWVIRDSDGSSICFGMKHIKRRWDINSLEALAIWEGLKCLSYFRKEEHLPLIIESDALEVVSCLNDQSQFLSKTKNIIDAIRSICSDWGSVEVVHCPRVENRVAHSIARLVAELSASFENTCTLTFSCEFLRLLFFFFCLFALGFYDIQFGSYILCRTVFLAVFLMASPVKNLAVSEEQKKECTVSNGKESEEVVGVLEVFVHQARDIHNICIYHKQDVYARLSFTTDPKYSVATKTINGGGRNPVFNESVRLDVRSIDTLLKCEIWMLSRVKNYLEDQLLGFALIPLTEVVVVNGKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPVMTAAPLETIAAVEDSNLIKSHPSDLDMIEFPDPKIENEDQIMVSEYFGISCSNLDSESSESLAISEGENHGISAVESYSITNEKSSEDSKIDSPPSSVQNDAVSSPSSESSEAPASSKSTTQEHVSAPNATSEKEQNAENGEVNGSSSEAAIDTITKPVVSVSIEPEDKVVQQDIVDMYMKSMQQFTESLAKMKLPLDIDTNGPPSSGSSSSDPKLQSPKNSGSRVFYGSRAFF
Homology
BLAST of Spg030603 vs. NCBI nr
Match: XP_038902970.1 (uncharacterized protein LOC120089682 [Benincasa hispida] >XP_038902971.1 uncharacterized protein LOC120089682 [Benincasa hispida])

HSP 1 Score: 620.9 bits (1600), Expect = 1.1e-173
Identity = 342/405 (84.44%), Postives = 364/405 (89.88%), Query Frame = 0

Query: 182 MASPVKNLAVSEEQKKECTVSNGKESEEVVGVLEVFVHQARDIHNICIYHKQDVYARLSF 241
           MASPV+ L V EE+KKEC V N KE +EVVGVLEVFVHQARDIHNICIYHKQDVYARLSF
Sbjct: 1   MASPVEKLGVLEEEKKECVVGNEKEGKEVVGVLEVFVHQARDIHNICIYHKQDVYARLSF 60

Query: 242 TTDPKYSVATKTINGGGRNPVFNESVRLDVRSIDTLLKCEIWMLSRVKNYLEDQLLGFAL 301
           TTDP  SV+TKTINGGGRNPVFNESVRLDVRSIDT+LKCEIWMLSRVKNYLEDQLLGFAL
Sbjct: 61  TTDPANSVSTKTINGGGRNPVFNESVRLDVRSIDTMLKCEIWMLSRVKNYLEDQLLGFAL 120

Query: 302 IPLTEVVVVNGKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPVMTAAPLETIA 361
           IPLTEVV V+ KLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAI       +ET  
Sbjct: 121 IPLTEVVAVDNKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAI-----RTIETTV 180

Query: 362 AVEDSNLIKSHPSDLDMIEFPDPKIENEDQIMVSEYFGISCSNLDSESSESLAISEGENH 421
            VEDS+L KSHPSDLDMIEFPDPKI NEDQIMVSEYFGI CSNLDSESSESL ISEGEN 
Sbjct: 181 GVEDSDLTKSHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLPISEGENQ 240

Query: 422 GISAVESYSITNEKSSEDSKIDSPPSSVQNDAVSSPSSESSEAPASSK-STTQEHVSAPN 481
           GI  V+++S++N+KSSE+SKIDSPPS+VQNDAVSSP+S  SEAPASSK +TTQEHVSAPN
Sbjct: 241 GILVVKNFSMSNDKSSEESKIDSPPSNVQNDAVSSPTS-PSEAPASSKCTTTQEHVSAPN 300

Query: 482 ATSEKEQNAENGEVNGSSSEAAIDTITKPVVSVSIEPEDKVVQQDIVDMYMKSMQQFTES 541
            TSEKEQN ENGEVN SS+EA   TITKP+VSVSIE EDKVVQQDIVDMYMKSMQQFTES
Sbjct: 301 ETSEKEQNGENGEVNDSSTEA---TITKPLVSVSIEAEDKVVQQDIVDMYMKSMQQFTES 360

Query: 542 LAKMKLPLDIDTNGPPSSGSSSSDPKLQSPKNSGSRVFYGSRAFF 586
           LAKMKLPLDIDTNG P+S +SSSDP LQSPKNSGSRVFYGSRAFF
Sbjct: 361 LAKMKLPLDIDTNGSPTSATSSSDPNLQSPKNSGSRVFYGSRAFF 396

BLAST of Spg030603 vs. NCBI nr
Match: XP_022156158.1 (uncharacterized protein LOC111023111 [Momordica charantia] >XP_022156159.1 uncharacterized protein LOC111023111 [Momordica charantia])

HSP 1 Score: 585.9 bits (1509), Expect = 3.9e-163
Identity = 333/416 (80.05%), Postives = 359/416 (86.30%), Query Frame = 0

Query: 182 MASPVKNLAVSEEQKKECTVSNGKESEEVVGVLEVFVHQARDIHNICIYHKQDVYARLSF 241
           MASPV NL VSE +K+EC VSNGKE E VVGVLEV VHQARDIHNICIYHKQDVYARLSF
Sbjct: 1   MASPVNNLGVSEHEKQECVVSNGKEGEGVVGVLEVLVHQARDIHNICIYHKQDVYARLSF 60

Query: 242 TTDPKYSVATKTINGGGRNPVFNESVRLDVRSIDTLLKCEIWMLSRVKNYLEDQLLGFAL 301
           T+DP  SV+TKTINGGGRNPVFNE+VRL VRS DT LKCEIWMLSRVKNYLEDQLLGFAL
Sbjct: 61  TSDPSNSVSTKTINGGGRNPVFNENVRLSVRSNDTSLKCEIWMLSRVKNYLEDQLLGFAL 120

Query: 302 IPLTEVV-VVNGKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPVMTAAPLETI 361
           IPL EVV  VNGKLEKEFSLSS+DLFHSPAGFVQLSLSYNGASPEVM IP      LETI
Sbjct: 121 IPLAEVVAAVNGKLEKEFSLSSSDLFHSPAGFVQLSLSYNGASPEVMEIPA-----LETI 180

Query: 362 AAVEDSNLIK-SHPSDLDMIEFPDPKIENEDQIMVSEYFGISCSNLDSESSESLAISEGE 421
           A V+DS++ K SHPSDLDMIEFPDPKI NEDQ+MVSEY GI CS+LDSE SESLAIS+GE
Sbjct: 181 ATVQDSDVNKLSHPSDLDMIEFPDPKIVNEDQMMVSEYIGIPCSDLDSERSESLAISDGE 240

Query: 422 NH-------GISAVESYSITNEKSSEDSKIDSPPSSVQNDAVSS--PSSESSEAPASSKS 481
           NH       GI  VE +S+ NEKS++ SKIDSPPSSV  DAVSS   SSESSEA ASSK+
Sbjct: 241 NHHLSPGIGGIPVVEKFSMPNEKSNQASKIDSPPSSVPTDAVSSLPASSESSEAAASSKA 300

Query: 482 TTQ-EHVSAPNATSEKEQNAENGEVNGSSSEAAIDTITKPVVSVSIEPEDKVVQQDIVDM 541
           TTQ E+VSA NA+SEKEQN ENGE+N S SEAA +TIT P+VSV+IEP+DKVVQQDIVDM
Sbjct: 301 TTQDEYVSAANASSEKEQNTENGEINDSCSEAAGETITNPIVSVNIEPDDKVVQQDIVDM 360

Query: 542 YMKSMQQFTESLAKMKLPLDIDTNGPPSSGSSSSDPKLQSPKNSGSRVFYGSRAFF 586
           YMKSMQQFTESLAKMKLPLDID +GPPSSGSS+SDP LQSPKNSG RVFYGSRAFF
Sbjct: 361 YMKSMQQFTESLAKMKLPLDID-SGPPSSGSSTSDPNLQSPKNSGPRVFYGSRAFF 410

BLAST of Spg030603 vs. NCBI nr
Match: XP_023005433.1 (uncharacterized protein LOC111498421 [Cucurbita maxima] >XP_023005434.1 uncharacterized protein LOC111498421 [Cucurbita maxima])

HSP 1 Score: 584.7 bits (1506), Expect = 8.7e-163
Identity = 327/409 (79.95%), Postives = 351/409 (85.82%), Query Frame = 0

Query: 182 MASPVKNLAVSEEQKKECTVSNGKESEEVVGVLEVFVHQARDIHNICIYHKQDVYARLSF 241
           MAS  KN+AVSEEQKKEC V NGKE++EVVGVLEV VHQARDIHNICIYHKQDVYARLSF
Sbjct: 1   MASEAKNMAVSEEQKKECVVENGKENKEVVGVLEVLVHQARDIHNICIYHKQDVYARLSF 60

Query: 242 TTDPKYSVATKTINGGGRNPVFNESVRLDVRSIDTLLKCEIWMLSRVKNYLEDQLLGFAL 301
           T DP+ SV+TKTINGGGRNPVFN+SVRLDVRSIDT LKCE+WMLSRVKNYLEDQLLGFAL
Sbjct: 61  TADPENSVSTKTINGGGRNPVFNDSVRLDVRSIDTSLKCELWMLSRVKNYLEDQLLGFAL 120

Query: 302 IPLTEVVVVNGKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPVMTAAPLETIA 361
           IPL E VVVNGKLEKEFSLSSTDLFHSPAGFVQLS+SYNGASPE MAIP +  APLE IA
Sbjct: 121 IPLRE-VVVNGKLEKEFSLSSTDLFHSPAGFVQLSISYNGASPEFMAIPAIATAPLEAIA 180

Query: 362 AVEDSNLIKSHPSDLDMIEFPDPKIENEDQIMVSEYFGISCSNLDSESSESLAISEGENH 421
             EDS+L KS PSDLDM+EFPD KI NEDQIMVSEY GI+CSNLDSESSE+L I +GEN 
Sbjct: 181 EGEDSSLTKSRPSDLDMMEFPDLKIANEDQIMVSEYIGITCSNLDSESSENLTIPKGEN- 240

Query: 422 GISAVESYSITNEKSSEDSKIDSPPSSVQNDAVSSP----SSESSEAPASSKSTTQEHVS 481
                  + ++NEKSSE SKIDSPP+SV NDAVSSP     SESSE  ASSK  TQEHVS
Sbjct: 241 -----PVFPMSNEKSSEASKIDSPPNSVSNDAVSSPPLPTCSESSETSASSKPATQEHVS 300

Query: 482 APNATSEKEQNAENGEVNGSSSEAAIDTITKPVVSVSIEP-EDKVVQQDIVDMYMKSMQQ 541
           AP        NA+NGEVN S S+AA DT+TKPVV VSIEP E+KVVQQDIVDMYMKSMQQ
Sbjct: 301 AP--------NAKNGEVNDSCSKAASDTVTKPVVCVSIEPEEEKVVQQDIVDMYMKSMQQ 360

Query: 542 FTESLAKMKLPLDIDTNGPPSSGSSSSDPKLQSPKNSGSRVFYGSRAFF 586
           FTESLAKMKLPLDIDTNGPPSSGSSSS+P LQSPKN+GSRVFYGSRAFF
Sbjct: 361 FTESLAKMKLPLDIDTNGPPSSGSSSSNPNLQSPKNNGSRVFYGSRAFF 394

BLAST of Spg030603 vs. NCBI nr
Match: KAG6596924.1 (hypothetical protein SDJN03_10104, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 583.6 bits (1503), Expect = 1.9e-162
Identity = 331/436 (75.92%), Postives = 359/436 (82.34%), Query Frame = 0

Query: 155 CLFALGFYDIQFGSYILCRTVFLAVFLMASPVKNLAVSEEQKKECTVSNGKESEEVVGVL 214
           C +  GF D          +V ++  LMA   KN+AVSEEQKKEC V NGKE++EVVGVL
Sbjct: 18  CFWIWGFSD----------SVLISGVLMAFEAKNMAVSEEQKKECVVENGKENKEVVGVL 77

Query: 215 EVFVHQARDIHNICIYHKQDVYARLSFTTDPKYSVATKTINGGGRNPVFNESVRLDVRSI 274
           EV VHQARDIHNICIYHKQDVYARLSFTTDP  SV+TKTINGGGRNPVFN+SVRLDVRSI
Sbjct: 78  EVLVHQARDIHNICIYHKQDVYARLSFTTDPANSVSTKTINGGGRNPVFNDSVRLDVRSI 137

Query: 275 DTLLKCEIWMLSRVKNYLEDQLLGFALIPLTEVVVVNGKLEKEFSLSSTDLFHSPAGFVQ 334
           DT LKCE+WMLSRVKNYLEDQLLGFALIPL EVVVVNGKLEKEFSLSSTDLFHSPAGFVQ
Sbjct: 138 DTSLKCELWMLSRVKNYLEDQLLGFALIPLREVVVVNGKLEKEFSLSSTDLFHSPAGFVQ 197

Query: 335 LSLSYNGASPEVMAIPVMTAAPLETIAAVEDSNLIKSHPSDLDMIEFPDPKIENEDQIMV 394
           LS+SY GASPE MAIP +  APLE IA  EDS+L KSHP+DLDM+EFPD KI NEDQIMV
Sbjct: 198 LSISYIGASPEFMAIPAIATAPLEAIAEGEDSSLTKSHPNDLDMMEFPDLKIANEDQIMV 257

Query: 395 SEYFGISCSNLDSESSESLAISEGENHGISAVESYSITNEKSSEDSKIDSPPSSVQNDAV 454
           SEY GI+CSNLDSESSE+L I +GEN        + ++NEKSSE SKIDSPP+SV NDAV
Sbjct: 258 SEYIGITCSNLDSESSENLTIPKGEN------PVFPMSNEKSSEASKIDSPPNSVSNDAV 317

Query: 455 SSPS----SESSEAPASSKSTTQEHVSAPNATSEKEQNAENGEVNGSSSEAAIDTITKPV 514
           SSPS    SES E  ASSK  TQ+HVSAP        NA+N EVN S SEAA DT+TKP 
Sbjct: 318 SSPSLPTCSESLETSASSKPATQDHVSAP--------NAKNSEVNYSCSEAASDTVTKPE 377

Query: 515 VSVSIEP-EDKVVQQDIVDMYMKSMQQFTESLAKMKLPLDIDTNGPPSSGSSSSDPKLQS 574
           V VSIEP E+KVVQQDIVDMYMKSMQQFTESLAKMKLPLDIDTNGPPSSGSSSS+P LQS
Sbjct: 378 VCVSIEPEEEKVVQQDIVDMYMKSMQQFTESLAKMKLPLDIDTNGPPSSGSSSSNPNLQS 429

Query: 575 PKNSGSRVFYGSRAFF 586
           PKN+GSRVFYGSRAFF
Sbjct: 438 PKNNGSRVFYGSRAFF 429

BLAST of Spg030603 vs. NCBI nr
Match: KAG7028404.1 (hypothetical protein SDJN02_09585, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 582.8 bits (1501), Expect = 3.3e-162
Identity = 325/410 (79.27%), Postives = 350/410 (85.37%), Query Frame = 0

Query: 181 LMASPVKNLAVSEEQKKECTVSNGKESEEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 240
           LMA   KN+AVSEEQKKEC V NGK+++EVVGVLEV VHQARDIHNICIYHKQDVYARLS
Sbjct: 8   LMAFEAKNMAVSEEQKKECVVENGKDNKEVVGVLEVLVHQARDIHNICIYHKQDVYARLS 67

Query: 241 FTTDPKYSVATKTINGGGRNPVFNESVRLDVRSIDTLLKCEIWMLSRVKNYLEDQLLGFA 300
           FTTDP  SV+TKTINGGGRNPVFN+SVRLDVRSIDT LKCE+WMLSRVKNYLEDQLLGFA
Sbjct: 68  FTTDPANSVSTKTINGGGRNPVFNDSVRLDVRSIDTSLKCELWMLSRVKNYLEDQLLGFA 127

Query: 301 LIPLTEVVVVNGKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPVMTAAPLETI 360
           LIPL EVVVVNGKLEKEFSLSSTDLFHSPAGFVQLS+SY GASPE MAIP +  APLE I
Sbjct: 128 LIPLREVVVVNGKLEKEFSLSSTDLFHSPAGFVQLSISYIGASPEFMAIPAIATAPLEAI 187

Query: 361 AAVEDSNLIKSHPSDLDMIEFPDPKIENEDQIMVSEYFGISCSNLDSESSESLAISEGEN 420
           A  EDS+L KSHP+DLDM+EFPD KI NEDQIMVSEY GI+CSNLDSESSE+L I +GEN
Sbjct: 188 AEGEDSSLTKSHPNDLDMMEFPDLKIANEDQIMVSEYIGITCSNLDSESSENLTIPKGEN 247

Query: 421 HGISAVESYSITNEKSSEDSKIDSPPSSVQNDAVSSPS----SESSEAPASSKSTTQEHV 480
                   + ++NEKSSE SKIDSPP+SV NDAVSSPS    SES E  ASSK  TQ+HV
Sbjct: 248 ------PVFPMSNEKSSEASKIDSPPNSVSNDAVSSPSLPTCSESLETSASSKPATQDHV 307

Query: 481 SAPNATSEKEQNAENGEVNGSSSEAAIDTITKPVVSVSIEP-EDKVVQQDIVDMYMKSMQ 540
           SAP        NA+N EVN S SEAA DT+TKP V VSIEP E+KVVQQDIVDMYMKSMQ
Sbjct: 308 SAP--------NAKNSEVNYSCSEAASDTVTKPEVCVSIEPEEEKVVQQDIVDMYMKSMQ 367

Query: 541 QFTESLAKMKLPLDIDTNGPPSSGSSSSDPKLQSPKNSGSRVFYGSRAFF 586
           QFTESLAKMKLPLDIDTNGPPSSGSSSS+P LQSPKN+GSRVFYGSRAFF
Sbjct: 368 QFTESLAKMKLPLDIDTNGPPSSGSSSSNPNLQSPKNNGSRVFYGSRAFF 403

BLAST of Spg030603 vs. ExPASy TrEMBL
Match: A0A6J1DSI6 (uncharacterized protein LOC111023111 OS=Momordica charantia OX=3673 GN=LOC111023111 PE=4 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.9e-163
Identity = 333/416 (80.05%), Postives = 359/416 (86.30%), Query Frame = 0

Query: 182 MASPVKNLAVSEEQKKECTVSNGKESEEVVGVLEVFVHQARDIHNICIYHKQDVYARLSF 241
           MASPV NL VSE +K+EC VSNGKE E VVGVLEV VHQARDIHNICIYHKQDVYARLSF
Sbjct: 1   MASPVNNLGVSEHEKQECVVSNGKEGEGVVGVLEVLVHQARDIHNICIYHKQDVYARLSF 60

Query: 242 TTDPKYSVATKTINGGGRNPVFNESVRLDVRSIDTLLKCEIWMLSRVKNYLEDQLLGFAL 301
           T+DP  SV+TKTINGGGRNPVFNE+VRL VRS DT LKCEIWMLSRVKNYLEDQLLGFAL
Sbjct: 61  TSDPSNSVSTKTINGGGRNPVFNENVRLSVRSNDTSLKCEIWMLSRVKNYLEDQLLGFAL 120

Query: 302 IPLTEVV-VVNGKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPVMTAAPLETI 361
           IPL EVV  VNGKLEKEFSLSS+DLFHSPAGFVQLSLSYNGASPEVM IP      LETI
Sbjct: 121 IPLAEVVAAVNGKLEKEFSLSSSDLFHSPAGFVQLSLSYNGASPEVMEIPA-----LETI 180

Query: 362 AAVEDSNLIK-SHPSDLDMIEFPDPKIENEDQIMVSEYFGISCSNLDSESSESLAISEGE 421
           A V+DS++ K SHPSDLDMIEFPDPKI NEDQ+MVSEY GI CS+LDSE SESLAIS+GE
Sbjct: 181 ATVQDSDVNKLSHPSDLDMIEFPDPKIVNEDQMMVSEYIGIPCSDLDSERSESLAISDGE 240

Query: 422 NH-------GISAVESYSITNEKSSEDSKIDSPPSSVQNDAVSS--PSSESSEAPASSKS 481
           NH       GI  VE +S+ NEKS++ SKIDSPPSSV  DAVSS   SSESSEA ASSK+
Sbjct: 241 NHHLSPGIGGIPVVEKFSMPNEKSNQASKIDSPPSSVPTDAVSSLPASSESSEAAASSKA 300

Query: 482 TTQ-EHVSAPNATSEKEQNAENGEVNGSSSEAAIDTITKPVVSVSIEPEDKVVQQDIVDM 541
           TTQ E+VSA NA+SEKEQN ENGE+N S SEAA +TIT P+VSV+IEP+DKVVQQDIVDM
Sbjct: 301 TTQDEYVSAANASSEKEQNTENGEINDSCSEAAGETITNPIVSVNIEPDDKVVQQDIVDM 360

Query: 542 YMKSMQQFTESLAKMKLPLDIDTNGPPSSGSSSSDPKLQSPKNSGSRVFYGSRAFF 586
           YMKSMQQFTESLAKMKLPLDID +GPPSSGSS+SDP LQSPKNSG RVFYGSRAFF
Sbjct: 361 YMKSMQQFTESLAKMKLPLDID-SGPPSSGSSTSDPNLQSPKNSGPRVFYGSRAFF 410

BLAST of Spg030603 vs. ExPASy TrEMBL
Match: A0A6J1L264 (uncharacterized protein LOC111498421 OS=Cucurbita maxima OX=3661 GN=LOC111498421 PE=4 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 4.2e-163
Identity = 327/409 (79.95%), Postives = 351/409 (85.82%), Query Frame = 0

Query: 182 MASPVKNLAVSEEQKKECTVSNGKESEEVVGVLEVFVHQARDIHNICIYHKQDVYARLSF 241
           MAS  KN+AVSEEQKKEC V NGKE++EVVGVLEV VHQARDIHNICIYHKQDVYARLSF
Sbjct: 1   MASEAKNMAVSEEQKKECVVENGKENKEVVGVLEVLVHQARDIHNICIYHKQDVYARLSF 60

Query: 242 TTDPKYSVATKTINGGGRNPVFNESVRLDVRSIDTLLKCEIWMLSRVKNYLEDQLLGFAL 301
           T DP+ SV+TKTINGGGRNPVFN+SVRLDVRSIDT LKCE+WMLSRVKNYLEDQLLGFAL
Sbjct: 61  TADPENSVSTKTINGGGRNPVFNDSVRLDVRSIDTSLKCELWMLSRVKNYLEDQLLGFAL 120

Query: 302 IPLTEVVVVNGKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPVMTAAPLETIA 361
           IPL E VVVNGKLEKEFSLSSTDLFHSPAGFVQLS+SYNGASPE MAIP +  APLE IA
Sbjct: 121 IPLRE-VVVNGKLEKEFSLSSTDLFHSPAGFVQLSISYNGASPEFMAIPAIATAPLEAIA 180

Query: 362 AVEDSNLIKSHPSDLDMIEFPDPKIENEDQIMVSEYFGISCSNLDSESSESLAISEGENH 421
             EDS+L KS PSDLDM+EFPD KI NEDQIMVSEY GI+CSNLDSESSE+L I +GEN 
Sbjct: 181 EGEDSSLTKSRPSDLDMMEFPDLKIANEDQIMVSEYIGITCSNLDSESSENLTIPKGEN- 240

Query: 422 GISAVESYSITNEKSSEDSKIDSPPSSVQNDAVSSP----SSESSEAPASSKSTTQEHVS 481
                  + ++NEKSSE SKIDSPP+SV NDAVSSP     SESSE  ASSK  TQEHVS
Sbjct: 241 -----PVFPMSNEKSSEASKIDSPPNSVSNDAVSSPPLPTCSESSETSASSKPATQEHVS 300

Query: 482 APNATSEKEQNAENGEVNGSSSEAAIDTITKPVVSVSIEP-EDKVVQQDIVDMYMKSMQQ 541
           AP        NA+NGEVN S S+AA DT+TKPVV VSIEP E+KVVQQDIVDMYMKSMQQ
Sbjct: 301 AP--------NAKNGEVNDSCSKAASDTVTKPVVCVSIEPEEEKVVQQDIVDMYMKSMQQ 360

Query: 542 FTESLAKMKLPLDIDTNGPPSSGSSSSDPKLQSPKNSGSRVFYGSRAFF 586
           FTESLAKMKLPLDIDTNGPPSSGSSSS+P LQSPKN+GSRVFYGSRAFF
Sbjct: 361 FTESLAKMKLPLDIDTNGPPSSGSSSSNPNLQSPKNNGSRVFYGSRAFF 394

BLAST of Spg030603 vs. ExPASy TrEMBL
Match: A0A6J1HDW1 (uncharacterized protein LOC111462642 OS=Cucurbita moschata OX=3662 GN=LOC111462642 PE=4 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 4.0e-161
Identity = 323/409 (78.97%), Postives = 349/409 (85.33%), Query Frame = 0

Query: 182 MASPVKNLAVSEEQKKECTVSNGKESEEVVGVLEVFVHQARDIHNICIYHKQDVYARLSF 241
           MAS  K++AVSEEQKKEC V NGK+++EVVGVLEV VHQARDIHNICIYHKQDVYARLSF
Sbjct: 1   MASEAKSMAVSEEQKKECVVENGKDNKEVVGVLEVLVHQARDIHNICIYHKQDVYARLSF 60

Query: 242 TTDPKYSVATKTINGGGRNPVFNESVRLDVRSIDTLLKCEIWMLSRVKNYLEDQLLGFAL 301
           TTDP  SV+TKTINGGGRNPVFN+SVRLDVRSIDT LKCE+WMLSRVKNYLEDQLLGFAL
Sbjct: 61  TTDPANSVSTKTINGGGRNPVFNDSVRLDVRSIDTSLKCELWMLSRVKNYLEDQLLGFAL 120

Query: 302 IPLTEVVVVNGKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPVMTAAPLETIA 361
           IPL EVVVVNGKLEKEFSLSSTDLFHSPAGFVQLS+SY GASPE MAIP +  APLE IA
Sbjct: 121 IPLREVVVVNGKLEKEFSLSSTDLFHSPAGFVQLSISYIGASPEFMAIPAIATAPLEAIA 180

Query: 362 AVEDSNLIKSHPSDLDMIEFPDPKIENEDQIMVSEYFGISCSNLDSESSESLAISEGENH 421
             EDS+L KSHP+DLDM+EFPD KI NEDQIMVSEY GI+CSNLDSESSE+  I + EN 
Sbjct: 181 EGEDSSLTKSHPNDLDMMEFPDLKIANEDQIMVSEYIGITCSNLDSESSENSTIPKCEN- 240

Query: 422 GISAVESYSITNEKSSEDSKIDSPPSSVQNDAVSSPS----SESSEAPASSKSTTQEHVS 481
                  + ++NEKSSE SKIDSPP+SV NDAVSSPS    SESS+  ASSK  TQEHVS
Sbjct: 241 -----PVFPMSNEKSSEASKIDSPPNSVSNDAVSSPSLPTCSESSKTSASSKPETQEHVS 300

Query: 482 APNATSEKEQNAENGEVNGSSSEAAIDTITKPVVSVSIEP-EDKVVQQDIVDMYMKSMQQ 541
           AP        NA+N EVN   SEAA DT+TKPVV VSIEP E+KVVQQDIVDMYMKSMQQ
Sbjct: 301 AP--------NAKNSEVNDLCSEAASDTVTKPVVCVSIEPEEEKVVQQDIVDMYMKSMQQ 360

Query: 542 FTESLAKMKLPLDIDTNGPPSSGSSSSDPKLQSPKNSGSRVFYGSRAFF 586
           FTESLAKMKLPLDIDTNGPPSSGSSSS+P LQSPKN+GSRVFYGSRAFF
Sbjct: 361 FTESLAKMKLPLDIDTNGPPSSGSSSSNPDLQSPKNNGSRVFYGSRAFF 395

BLAST of Spg030603 vs. ExPASy TrEMBL
Match: A0A5A7VFA2 (C2 calcium-dependent membrane targeting OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001780 PE=4 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 1.1e-155
Identity = 324/409 (79.22%), Postives = 347/409 (84.84%), Query Frame = 0

Query: 182 MASPVKNLAVSEEQKKEC-TVSNGKESEEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 241
           MA+PV+ L V EE+KKEC  V N KE  EVVGVLEVFVHQARDIHNICIYHKQDVYARLS
Sbjct: 1   MATPVEKLGVLEEEKKECVVVGNEKERNEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 60

Query: 242 FTTDPKYSVATKTINGGGRNPVFNESVRLDVRSIDTLLKCEIWMLSRVKNYLEDQLLGFA 301
           FTTDP  SV+TKTINGGGRNPVFN++VRLDVRSIDT LKCEIWMLSRVKNYLEDQLLGFA
Sbjct: 61  FTTDPTNSVSTKTINGGGRNPVFNDTVRLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA 120

Query: 302 LIPLTEVVVVNGKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPVMTAAPLETI 361
           LIPLTEVV V+ KLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVM IP      +ET 
Sbjct: 121 LIPLTEVVAVDNKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMVIPT-----IETT 180

Query: 362 AAVEDSNLIK-SHPSDLDMIEFPDPKIENEDQIMVSEYFGISCSNLDSESSESLAISEGE 421
             VE+S+LIK SHPSDLDMIEFPDPKI NEDQIMVSEYFGI CSNLDSESSESL +SE E
Sbjct: 181 VGVENSDLIKSSHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLPVSESE 240

Query: 422 NHG-ISAVESYSITNEKSSEDSKIDSPPSSVQNDAV-SSPSSESSEAPASSKSTTQEHVS 481
           NH  ISAVES   +N+K SED+KIDSP S +QN+AV SSPS  + EAP +SK TTQE VS
Sbjct: 241 NHHVISAVES---SNDKLSEDTKIDSPLSKMQNNAVSSSPSPSALEAPVNSKCTTQEEVS 300

Query: 482 APNATSEKEQNAENGEVNGSSSEAAIDTITKPVVSVSIEPEDKVVQQDIVDMYMKSMQQF 541
           APN TSEKE+  +NGEVN   S    DTI KP+VSVSIE E+KVVQQDIVD YMKSMQQF
Sbjct: 301 APNGTSEKEE--KNGEVNDCCS----DTIRKPLVSVSIEAENKVVQQDIVDKYMKSMQQF 360

Query: 542 TESLAKMKLPLDIDTN-GPPSSGSSSSDPKLQSPKNSGSRVFYGSRAFF 586
           TESLAKMKLPLDIDTN G PS  +SSSDP LQSPKNSGSRVFYGSRAFF
Sbjct: 361 TESLAKMKLPLDIDTNGGSPSPATSSSDPNLQSPKNSGSRVFYGSRAFF 395

BLAST of Spg030603 vs. ExPASy TrEMBL
Match: A0A1S3BL87 (uncharacterized protein LOC103491056 OS=Cucumis melo OX=3656 GN=LOC103491056 PE=4 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.9e-155
Identity = 325/409 (79.46%), Postives = 344/409 (84.11%), Query Frame = 0

Query: 182 MASPVKNLAVSEEQKKEC-TVSNGKESEEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 241
           MA+PV+ L V EE+KKEC  V N KE  EVVGVLEVFVHQARDIHNICIYHKQDVYARLS
Sbjct: 1   MATPVEKLGVLEEEKKECVVVGNEKERNEVVGVLEVFVHQARDIHNICIYHKQDVYARLS 60

Query: 242 FTTDPKYSVATKTINGGGRNPVFNESVRLDVRSIDTLLKCEIWMLSRVKNYLEDQLLGFA 301
           FTTDP  SV+TKTINGGGRNPVFN++VRLDVRSIDT LKCEIWMLSRVKNYLEDQLLGFA
Sbjct: 61  FTTDPTNSVSTKTINGGGRNPVFNDTVRLDVRSIDTTLKCEIWMLSRVKNYLEDQLLGFA 120

Query: 302 LIPLTEVVVVNGKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPVMTAAPLETI 361
           LIPLTEVV V+ KLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVM IP      +ET 
Sbjct: 121 LIPLTEVVAVDNKLEKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMVIPT-----IETT 180

Query: 362 AAVEDSNLIK-SHPSDLDMIEFPDPKIENEDQIMVSEYFGISCSNLDSESSESLAISEGE 421
             VE+S+LIK SHPSDLDMIEFPDPKI NEDQIMVSEYFGI CSNLDSESSESL +SE E
Sbjct: 181 VGVENSDLIKSSHPSDLDMIEFPDPKIANEDQIMVSEYFGIPCSNLDSESSESLPVSESE 240

Query: 422 NHG-ISAVESYSITNEKSSEDSKIDSPPSSVQNDAV-SSPSSESSEAPASSKSTTQEHVS 481
           NH  ISAVES       SSED+KIDSP S VQNDAV SSPS  + EAP +SK TTQE VS
Sbjct: 241 NHHVISAVES------SSSEDTKIDSPLSKVQNDAVSSSPSPSALEAPVNSKCTTQEEVS 300

Query: 482 APNATSEKEQNAENGEVNGSSSEAAIDTITKPVVSVSIEPEDKVVQQDIVDMYMKSMQQF 541
           APN TSEKE+  +NGEVN   S    DTI KP+VSVSIE E+KVVQQDIVD YMKSMQQF
Sbjct: 301 APNGTSEKEE--KNGEVNDCCS----DTIRKPLVSVSIEAENKVVQQDIVDKYMKSMQQF 360

Query: 542 TESLAKMKLPLDIDTN-GPPSSGSSSSDPKLQSPKNSGSRVFYGSRAFF 586
           TESLAKMKLPLDIDTN G PS  +SSSDP LQSPKNSGSRVFYGSRAFF
Sbjct: 361 TESLAKMKLPLDIDTNGGSPSPATSSSDPNLQSPKNSGSRVFYGSRAFF 392

BLAST of Spg030603 vs. TAIR 10
Match: AT5G55530.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 384.4 bits (986), Expect = 1.6e-106
Identity = 226/391 (57.80%), Postives = 290/391 (74.17%), Query Frame = 0

Query: 202 SNGKES-----EEVVGVLEVFVHQARDIHNICIYHKQDVYARLSFTTDPKYSVATKTING 261
           SNGK+S     +++VG LEV+VHQARDIHNICIYHKQDVYA+L  T+DP  SV+TK ING
Sbjct: 36  SNGKDSKSCGRQDLVGALEVYVHQARDIHNICIYHKQDVYAKLCLTSDPDKSVSTKIING 95

Query: 262 GGRNPVFNESVRLDVRSIDTLLKCEIWMLSRVKNYLEDQLLGFALIPLTEVVVVNGKLEK 321
           GGRNPVF+++V+LDVR +DT LKCEI+M+SRVKNYLEDQLLGF L+P++E++  NGKLEK
Sbjct: 96  GGRNPVFDDNVKLDVRVLDTSLKCEIYMMSRVKNYLEDQLLGFTLVPMSELLFKNGKLEK 155

Query: 322 EFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPVMTAAPLETIAAVEDSNLIKSHPSDL 381
           EFSLSSTDL+HSPAGFVQLSLSY G+ P+VMAIP M ++ +      +D    +S P +L
Sbjct: 156 EFSLSSTDLYHSPAGFVQLSLSYYGSYPDVMAIPSMPSS-VSIDETTKDPEGSESVPGEL 215

Query: 382 DMIEFPDPKIENEDQIMVSEYFGISCSNLDSESSESLAISEGENHGISAVESYSITNEKS 441
           D IEFPDP + NE++ MVSEYFGISCS +DSE+S+SL  S+ ENH  ++V S        
Sbjct: 216 DKIEFPDPNVANENEKMVSEYFGISCSTIDSETSDSLVTSDAENHVTNSVTSI------- 275

Query: 442 SEDSKIDSPPSSVQNDAVSSPSSESSEAPASSKSTTQEHVSAPNATSEKEQNAENGEVNG 501
               K DSP SS   +A +  +S  + A +++++   EH+S  N+ +  +++    E +G
Sbjct: 276 ---LKQDSPESS---NATNGAASPHASAHSATETPNHEHLSVVNSKASSQES--ESEASG 335

Query: 502 SSSEAAIDTITKPVVSVSIEPEDKVVQQDIVDMYMKSMQQFTESLAKMKLPLDIDTNGPP 561
            +SE   +   K V++V +EPE KVVQQDIVDMYMKSMQQFT+SLAKMKLPLDID+  P 
Sbjct: 336 ETSE---EKTVKSVLTVKVEPESKVVQQDIVDMYMKSMQQFTDSLAKMKLPLDIDS--PT 395

Query: 562 SSGSSSSD-PKLQSPK-NSGSRVFYGSRAFF 586
            S +SSSD  KL +PK N+GSRVFYGSR FF
Sbjct: 396 KSENSSSDSQKLPTPKSNNGSRVFYGSRPFF 405

BLAST of Spg030603 vs. TAIR 10
Match: AT5G55530.3 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 384.4 bits (986), Expect = 1.6e-106
Identity = 226/391 (57.80%), Postives = 290/391 (74.17%), Query Frame = 0

Query: 202 SNGKES-----EEVVGVLEVFVHQARDIHNICIYHKQDVYARLSFTTDPKYSVATKTING 261
           SNGK+S     +++VG LEV+VHQARDIHNICIYHKQDVYA+L  T+DP  SV+TK ING
Sbjct: 36  SNGKDSKSCGRQDLVGALEVYVHQARDIHNICIYHKQDVYAKLCLTSDPDKSVSTKIING 95

Query: 262 GGRNPVFNESVRLDVRSIDTLLKCEIWMLSRVKNYLEDQLLGFALIPLTEVVVVNGKLEK 321
           GGRNPVF+++V+LDVR +DT LKCEI+M+SRVKNYLEDQLLGF L+P++E++  NGKLEK
Sbjct: 96  GGRNPVFDDNVKLDVRVLDTSLKCEIYMMSRVKNYLEDQLLGFTLVPMSELLFKNGKLEK 155

Query: 322 EFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPVMTAAPLETIAAVEDSNLIKSHPSDL 381
           EFSLSSTDL+HSPAGFVQLSLSY G+ P+VMAIP M ++ +      +D    +S P +L
Sbjct: 156 EFSLSSTDLYHSPAGFVQLSLSYYGSYPDVMAIPSMPSS-VSIDETTKDPEGSESVPGEL 215

Query: 382 DMIEFPDPKIENEDQIMVSEYFGISCSNLDSESSESLAISEGENHGISAVESYSITNEKS 441
           D IEFPDP + NE++ MVSEYFGISCS +DSE+S+SL  S+ ENH  ++V S        
Sbjct: 216 DKIEFPDPNVANENEKMVSEYFGISCSTIDSETSDSLVTSDAENHVTNSVTSI------- 275

Query: 442 SEDSKIDSPPSSVQNDAVSSPSSESSEAPASSKSTTQEHVSAPNATSEKEQNAENGEVNG 501
               K DSP SS   +A +  +S  + A +++++   EH+S  N+ +  +++    E +G
Sbjct: 276 ---LKQDSPESS---NATNGAASPHASAHSATETPNHEHLSVVNSKASSQES--ESEASG 335

Query: 502 SSSEAAIDTITKPVVSVSIEPEDKVVQQDIVDMYMKSMQQFTESLAKMKLPLDIDTNGPP 561
            +SE   +   K V++V +EPE KVVQQDIVDMYMKSMQQFT+SLAKMKLPLDID+  P 
Sbjct: 336 ETSE---EKTVKSVLTVKVEPESKVVQQDIVDMYMKSMQQFTDSLAKMKLPLDIDS--PT 395

Query: 562 SSGSSSSD-PKLQSPK-NSGSRVFYGSRAFF 586
            S +SSSD  KL +PK N+GSRVFYGSR FF
Sbjct: 396 KSENSSSDSQKLPTPKSNNGSRVFYGSRPFF 405

BLAST of Spg030603 vs. TAIR 10
Match: AT5G55530.2 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 384.4 bits (986), Expect = 1.6e-106
Identity = 226/391 (57.80%), Postives = 290/391 (74.17%), Query Frame = 0

Query: 202 SNGKES-----EEVVGVLEVFVHQARDIHNICIYHKQDVYARLSFTTDPKYSVATKTING 261
           SNGK+S     +++VG LEV+VHQARDIHNICIYHKQDVYA+L  T+DP  SV+TK ING
Sbjct: 36  SNGKDSKSCGRQDLVGALEVYVHQARDIHNICIYHKQDVYAKLCLTSDPDKSVSTKIING 95

Query: 262 GGRNPVFNESVRLDVRSIDTLLKCEIWMLSRVKNYLEDQLLGFALIPLTEVVVVNGKLEK 321
           GGRNPVF+++V+LDVR +DT LKCEI+M+SRVKNYLEDQLLGF L+P++E++  NGKLEK
Sbjct: 96  GGRNPVFDDNVKLDVRVLDTSLKCEIYMMSRVKNYLEDQLLGFTLVPMSELLFKNGKLEK 155

Query: 322 EFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPVMTAAPLETIAAVEDSNLIKSHPSDL 381
           EFSLSSTDL+HSPAGFVQLSLSY G+ P+VMAIP M ++ +      +D    +S P +L
Sbjct: 156 EFSLSSTDLYHSPAGFVQLSLSYYGSYPDVMAIPSMPSS-VSIDETTKDPEGSESVPGEL 215

Query: 382 DMIEFPDPKIENEDQIMVSEYFGISCSNLDSESSESLAISEGENHGISAVESYSITNEKS 441
           D IEFPDP + NE++ MVSEYFGISCS +DSE+S+SL  S+ ENH  ++V S        
Sbjct: 216 DKIEFPDPNVANENEKMVSEYFGISCSTIDSETSDSLVTSDAENHVTNSVTSI------- 275

Query: 442 SEDSKIDSPPSSVQNDAVSSPSSESSEAPASSKSTTQEHVSAPNATSEKEQNAENGEVNG 501
               K DSP SS   +A +  +S  + A +++++   EH+S  N+ +  +++    E +G
Sbjct: 276 ---LKQDSPESS---NATNGAASPHASAHSATETPNHEHLSVVNSKASSQES--ESEASG 335

Query: 502 SSSEAAIDTITKPVVSVSIEPEDKVVQQDIVDMYMKSMQQFTESLAKMKLPLDIDTNGPP 561
            +SE   +   K V++V +EPE KVVQQDIVDMYMKSMQQFT+SLAKMKLPLDID+  P 
Sbjct: 336 ETSE---EKTVKSVLTVKVEPESKVVQQDIVDMYMKSMQQFTDSLAKMKLPLDIDS--PT 395

Query: 562 SSGSSSSD-PKLQSPK-NSGSRVFYGSRAFF 586
            S +SSSD  KL +PK N+GSRVFYGSR FF
Sbjct: 396 KSENSSSDSQKLPTPKSNNGSRVFYGSRPFF 405

BLAST of Spg030603 vs. TAIR 10
Match: AT1G50570.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 337.0 bits (863), Expect = 3.0e-92
Identity = 202/392 (51.53%), Postives = 266/392 (67.86%), Query Frame = 0

Query: 195 QKKECTVSNGKESEEVVGVLEVFVHQARDIHNICIYHKQDVYARLSFTTDPKYSVATKTI 254
           + K   +    +S+  +GVLEVFVHQARDIHNICIYHKQDVYA+L  T DP+ S++TK I
Sbjct: 22  ETKTKNIVMSSDSDSFIGVLEVFVHQARDIHNICIYHKQDVYAKLCLTNDPENSLSTKII 81

Query: 255 NGGGRNPVFNESVRLDVRSIDTLLKCEIWMLSRVKNYLEDQLLGFALIPLTEVVVVNGKL 314
           NGGG+NPVF+++++ DV+++D  LKCEI+M+SRVKNYLEDQLLGF+L+PL+EV+V NGKL
Sbjct: 82  NGGGQNPVFDDTLQFDVKNLDCSLKCEIFMMSRVKNYLEDQLLGFSLVPLSEVIVRNGKL 141

Query: 315 EKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPVMTAAPLETIAAVEDSNLIKSHPS 374
           EKEFSLSSTDL+HSPAGFV+LSLSY G SP+VM IP +  A    +A           P 
Sbjct: 142 EKEFSLSSTDLYHSPAGFVELSLSYAGDSPDVMHIPAVPTADETELA-----------PI 201

Query: 375 DLDMIEFPDPKIENEDQIMVSEYFGISCSNLDSESSESLAISEGENHGISAVESYSITNE 434
           + D  EF DPKI  E+  MVS+YF  +CS+ D  +S      E  N  +SAV   ++   
Sbjct: 202 EFDESEFLDPKIVCENNQMVSKYFSTTCSDSDDFASSETGFVE-VNSILSAVVETAVDEA 261

Query: 435 KSSEDSKIDSPPSSVQNDAVSSPSSESSEAPASSKSTTQEHVSAPNATSEKEQNAENGEV 494
                    +P +SV  + +SSPS+  S   + +   +++     N+ SE+E       +
Sbjct: 262 ---------APANSVSTNGISSPSTAVSSGSSGTHDVSKQSSEGNNSDSEQEAKKPTDII 321

Query: 495 -NGSSSEAAIDTITKPVVSVSIEPEDKVVQQDIVDMYMKSMQQFTESLAKMKLPLDIDTN 554
            +G   +   + + KPV++V+IEPE KVVQQDIVDMY KS+QQFTESLAKMKLPLDID+ 
Sbjct: 322 KSGDLDKTDEEAVVKPVLTVNIEPEQKVVQQDIVDMYTKSLQQFTESLAKMKLPLDIDS- 381

Query: 555 GPPSSGSSSSDPKLQSPKNSGSRVFYGSRAFF 586
            P  S +SSS    Q+PK++ SRVFYGSRAFF
Sbjct: 382 -PTQSENSSSSQ--QTPKSASSRVFYGSRAFF 388

BLAST of Spg030603 vs. TAIR 10
Match: AT1G50570.2 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 337.0 bits (863), Expect = 3.0e-92
Identity = 202/392 (51.53%), Postives = 266/392 (67.86%), Query Frame = 0

Query: 195 QKKECTVSNGKESEEVVGVLEVFVHQARDIHNICIYHKQDVYARLSFTTDPKYSVATKTI 254
           + K   +    +S+  +GVLEVFVHQARDIHNICIYHKQDVYA+L  T DP+ S++TK I
Sbjct: 22  ETKTKNIVMSSDSDSFIGVLEVFVHQARDIHNICIYHKQDVYAKLCLTNDPENSLSTKII 81

Query: 255 NGGGRNPVFNESVRLDVRSIDTLLKCEIWMLSRVKNYLEDQLLGFALIPLTEVVVVNGKL 314
           NGGG+NPVF+++++ DV+++D  LKCEI+M+SRVKNYLEDQLLGF+L+PL+EV+V NGKL
Sbjct: 82  NGGGQNPVFDDTLQFDVKNLDCSLKCEIFMMSRVKNYLEDQLLGFSLVPLSEVIVRNGKL 141

Query: 315 EKEFSLSSTDLFHSPAGFVQLSLSYNGASPEVMAIPVMTAAPLETIAAVEDSNLIKSHPS 374
           EKEFSLSSTDL+HSPAGFV+LSLSY G SP+VM IP +  A    +A           P 
Sbjct: 142 EKEFSLSSTDLYHSPAGFVELSLSYAGDSPDVMHIPAVPTADETELA-----------PI 201

Query: 375 DLDMIEFPDPKIENEDQIMVSEYFGISCSNLDSESSESLAISEGENHGISAVESYSITNE 434
           + D  EF DPKI  E+  MVS+YF  +CS+ D  +S      E  N  +SAV   ++   
Sbjct: 202 EFDESEFLDPKIVCENNQMVSKYFSTTCSDSDDFASSETGFVE-VNSILSAVVETAVDEA 261

Query: 435 KSSEDSKIDSPPSSVQNDAVSSPSSESSEAPASSKSTTQEHVSAPNATSEKEQNAENGEV 494
                    +P +SV  + +SSPS+  S   + +   +++     N+ SE+E       +
Sbjct: 262 ---------APANSVSTNGISSPSTAVSSGSSGTHDVSKQSSEGNNSDSEQEAKKPTDII 321

Query: 495 -NGSSSEAAIDTITKPVVSVSIEPEDKVVQQDIVDMYMKSMQQFTESLAKMKLPLDIDTN 554
            +G   +   + + KPV++V+IEPE KVVQQDIVDMY KS+QQFTESLAKMKLPLDID+ 
Sbjct: 322 KSGDLDKTDEEAVVKPVLTVNIEPEQKVVQQDIVDMYTKSLQQFTESLAKMKLPLDIDS- 381

Query: 555 GPPSSGSSSSDPKLQSPKNSGSRVFYGSRAFF 586
            P  S +SSS    Q+PK++ SRVFYGSRAFF
Sbjct: 382 -PTQSENSSSSQ--QTPKSASSRVFYGSRAFF 388

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902970.11.1e-17384.44uncharacterized protein LOC120089682 [Benincasa hispida] >XP_038902971.1 unchara... [more]
XP_022156158.13.9e-16380.05uncharacterized protein LOC111023111 [Momordica charantia] >XP_022156159.1 uncha... [more]
XP_023005433.18.7e-16379.95uncharacterized protein LOC111498421 [Cucurbita maxima] >XP_023005434.1 uncharac... [more]
KAG6596924.11.9e-16275.92hypothetical protein SDJN03_10104, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7028404.13.3e-16279.27hypothetical protein SDJN02_09585, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DSI61.9e-16380.05uncharacterized protein LOC111023111 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1L2644.2e-16379.95uncharacterized protein LOC111498421 OS=Cucurbita maxima OX=3661 GN=LOC111498421... [more]
A0A6J1HDW14.0e-16178.97uncharacterized protein LOC111462642 OS=Cucurbita moschata OX=3662 GN=LOC1114626... [more]
A0A5A7VFA21.1e-15579.22C2 calcium-dependent membrane targeting OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3BL871.9e-15579.46uncharacterized protein LOC103491056 OS=Cucumis melo OX=3656 GN=LOC103491056 PE=... [more]
Match NameE-valueIdentityDescription
AT5G55530.11.6e-10657.80Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT5G55530.31.6e-10657.80Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT5G55530.21.6e-10657.80Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT1G50570.13.0e-9251.53Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT1G50570.23.0e-9251.53Calcium-dependent lipid-binding (CaLB domain) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000008C2 domainSMARTSM00239C2_3ccoord: 213..316
e-value: 6.9E-6
score: 35.6
IPR000008C2 domainPFAMPF00168C2coord: 212..308
e-value: 2.8E-8
score: 34.0
IPR000008C2 domainPROSITEPS50004C2coord: 195..318
score: 13.226864
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 2..125
e-value: 3.2E-20
score: 72.2
IPR035892C2 domain superfamilyGENE3D2.60.40.150C2 domaincoord: 207..348
e-value: 5.9E-15
score: 57.6
IPR035892C2 domain superfamilySUPERFAMILY49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 209..342
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..133
e-value: 5.8E-14
score: 54.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 546..585
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 442..505
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 553..576
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 432..518
NoneNo IPR availablePANTHERPTHR31208EXPRESSED PROTEINcoord: 193..585
NoneNo IPR availablePANTHERPTHR31208:SF2DOMAIN-CONTAINING PROTEIN, PUTATIVE, EXPRESSED-RELATEDcoord: 193..585
NoneNo IPR availableCDDcd00030C2coord: 214..320
e-value: 1.33115E-11
score: 59.3879
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 2..125
e-value: 1.22373E-25
score: 100.081
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 2..130

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg030603.1Spg030603.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity