Spg030521 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg030521
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionDNA mismatch repair protein MSH5
Locationscaffold6: 30021518 .. 30022411 (+)
RNA-Seq ExpressionSpg030521
SyntenySpg030521
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGTGCTCTGATACCAATTTGTTAGGTATCTTCTCTCAATATCCTCTGAAACAATCAGAATAATACACACAAATACCCAACAACAGCAATTTGACACTGTTATATTGATATGGATGCAATACACAGTGCAACATCAAGAGTCTAGCATCAACAAAAGAGAAATAAGCCAGAAAACAACCAAAGAAACAACCAAACACTACCCAGCTATTTCGAGAGAGTTGGGATGTCTCCCAAGGCTCTCCTCTCAGCCGAAAACAACTTTCCCCCACAAAGATTCCCTACACCCCTACAAAACCCCTCCCAACCAACAAACAAACTGTACAAGCAACACTCCACACTACATTTCTGTAACAGTCCCCACCAGCCTCCCTCAAACATATGCATTTCCATCTTTACCCTTTCTCTTGTATGTGTGTATAATCAGAGGCCTAACAAACACTCAACCCAAATATTTCCAAAGTTAATGCTTAGTTTGCTTTAGAATTTTCCCGTCAAAAAGAAGCTTGTGGTACTGTAAATTACAGTGATATTCTTGCTCCCATTACTGCTGCAAACTGATCCTATTACTTGTATAGGCGTTCCAGATGAGGTTATTAAGAGAGCAGCCTCTGTTTTGGATGCCATAGGGAATAATAAGCATGTTGAGCGGCTGCACAACGAGAATTTATCCGCTCATGATAAGCTATACCAGGTCTTTTATGACTTTGAATTTTCCTTGGGTTGTAGTTATTTGTGTAGTTTGCAATACTTATGATTCCTTATCAGTTGTTGACAGAATGATGATGTGATTGAACTCTGAACTAAATACAGGATGCGGTCGACAAGTTGCTGGGGCTTGATGTTAACAAGTGTGATCTTAGCCTTTTCTTTCGGGACATATTTCCTTCGTAA

mRNA sequence

ATGACGTGCTCTGATACCAATTTGTTAGGCGTTCCAGATGAGGTTATTAAGAGAGCAGCCTCTGTTTTGGATGCCATAGGGAATAATAAGCATGTTGAGCGGCTGCACAACGAGAATTTATCCGCTCATGATAAGCTATACCAGGATGCGGTCGACAAGTTGCTGGGGCTTGATGTTAACAAGTGTGATCTTAGCCTTTTCTTTCGGGACATATTTCCTTCGTAA

Coding sequence (CDS)

ATGACGTGCTCTGATACCAATTTGTTAGGCGTTCCAGATGAGGTTATTAAGAGAGCAGCCTCTGTTTTGGATGCCATAGGGAATAATAAGCATGTTGAGCGGCTGCACAACGAGAATTTATCCGCTCATGATAAGCTATACCAGGATGCGGTCGACAAGTTGCTGGGGCTTGATGTTAACAAGTGTGATCTTAGCCTTTTCTTTCGGGACATATTTCCTTCGTAA

Protein sequence

MTCSDTNLLGVPDEVIKRAASVLDAIGNNKHVERLHNENLSAHDKLYQDAVDKLLGLDVNKCDLSLFFRDIFPS
Homology
BLAST of Spg030521 vs. NCBI nr
Match: XP_023553301.1 (DNA mismatch repair protein MSH5 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023553302.1 DNA mismatch repair protein MSH5 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 122.5 bits (306), Expect = 1.6e-24
Identity = 60/67 (89.55%), Postives = 62/67 (92.54%), Query Frame = 0

Query: 8   LLGVPDEVIKRAASVLDAIGNNKHVERLHNENLSAHDKLYQDAVDKLLGLDVNKCDLSLF 67
           L GVPDEVIKRAA VLDA+GNNKHVERLHNENLSA DKLYQDAVDKLLGLDVNKCDLS F
Sbjct: 742 LAGVPDEVIKRAAFVLDAMGNNKHVERLHNENLSAQDKLYQDAVDKLLGLDVNKCDLSRF 801

Query: 68  FRDIFPS 75
           F+ IFPS
Sbjct: 802 FQGIFPS 808

BLAST of Spg030521 vs. NCBI nr
Match: XP_023007704.1 (DNA mismatch repair protein MSH5 isoform X7 [Cucurbita maxima])

HSP 1 Score: 122.5 bits (306), Expect = 1.6e-24
Identity = 60/67 (89.55%), Postives = 62/67 (92.54%), Query Frame = 0

Query: 8   LLGVPDEVIKRAASVLDAIGNNKHVERLHNENLSAHDKLYQDAVDKLLGLDVNKCDLSLF 67
           L GVPDEVIKRAA VLDA+GNNKHVERLHNENLSA DKLYQDAVDKLLGLDVNKCDLS F
Sbjct: 680 LAGVPDEVIKRAAFVLDAMGNNKHVERLHNENLSAQDKLYQDAVDKLLGLDVNKCDLSRF 739

Query: 68  FRDIFPS 75
           F+ IFPS
Sbjct: 740 FQGIFPS 746

BLAST of Spg030521 vs. NCBI nr
Match: XP_023007699.1 (DNA mismatch repair protein MSH5 isoform X2 [Cucurbita maxima])

HSP 1 Score: 122.5 bits (306), Expect = 1.6e-24
Identity = 60/67 (89.55%), Postives = 62/67 (92.54%), Query Frame = 0

Query: 8   LLGVPDEVIKRAASVLDAIGNNKHVERLHNENLSAHDKLYQDAVDKLLGLDVNKCDLSLF 67
           L GVPDEVIKRAA VLDA+GNNKHVERLHNENLSA DKLYQDAVDKLLGLDVNKCDLS F
Sbjct: 759 LAGVPDEVIKRAAFVLDAMGNNKHVERLHNENLSAQDKLYQDAVDKLLGLDVNKCDLSRF 818

Query: 68  FRDIFPS 75
           F+ IFPS
Sbjct: 819 FQGIFPS 825

BLAST of Spg030521 vs. NCBI nr
Match: XP_023007696.1 (DNA mismatch repair protein MSH5 isoform X1 [Cucurbita maxima] >XP_023007698.1 DNA mismatch repair protein MSH5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 122.5 bits (306), Expect = 1.6e-24
Identity = 60/67 (89.55%), Postives = 62/67 (92.54%), Query Frame = 0

Query: 8   LLGVPDEVIKRAASVLDAIGNNKHVERLHNENLSAHDKLYQDAVDKLLGLDVNKCDLSLF 67
           L GVPDEVIKRAA VLDA+GNNKHVERLHNENLSA DKLYQDAVDKLLGLDVNKCDLS F
Sbjct: 760 LAGVPDEVIKRAAFVLDAMGNNKHVERLHNENLSAQDKLYQDAVDKLLGLDVNKCDLSRF 819

Query: 68  FRDIFPS 75
           F+ IFPS
Sbjct: 820 FQGIFPS 826

BLAST of Spg030521 vs. NCBI nr
Match: XP_022932275.1 (DNA mismatch repair protein MSH5 isoform X6 [Cucurbita moschata])

HSP 1 Score: 122.5 bits (306), Expect = 1.6e-24
Identity = 60/67 (89.55%), Postives = 62/67 (92.54%), Query Frame = 0

Query: 8   LLGVPDEVIKRAASVLDAIGNNKHVERLHNENLSAHDKLYQDAVDKLLGLDVNKCDLSLF 67
           L GVPDEVIKRAA VLDA+GNNKHVERLHNENLSA DKLYQDAVDKLLGLDVNKCDLS F
Sbjct: 680 LAGVPDEVIKRAAFVLDAMGNNKHVERLHNENLSAQDKLYQDAVDKLLGLDVNKCDLSRF 739

Query: 68  FRDIFPS 75
           F+ IFPS
Sbjct: 740 FQGIFPS 746

BLAST of Spg030521 vs. ExPASy Swiss-Prot
Match: F4JEP5 (DNA mismatch repair protein MSH5 OS=Arabidopsis thaliana OX=3702 GN=MSH5 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.4e-12
Identity = 34/67 (50.75%), Postives = 50/67 (74.63%), Query Frame = 0

Query: 8   LLGVPDEVIKRAASVLDAIGNNKHVERLHNENLSAHDKLYQDAVDKLLGLDVNKCDLSLF 67
           L GVP+EV+KRAA VLDA  +N +V++L  + +S+ D+ ++DAVDK   LD++K D+  F
Sbjct: 741 LAGVPEEVVKRAAIVLDAFESNNNVDKLSLDKISSQDQAFKDAVDKFAELDISKGDIHAF 800

Query: 68  FRDIFPS 75
           F+DIF S
Sbjct: 801 FQDIFTS 807

BLAST of Spg030521 vs. ExPASy Swiss-Prot
Match: Q6L4V0 (DNA mismatch repair protein MSH5 OS=Oryza sativa subsp. japonica OX=39947 GN=MSH5 PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 3.0e-10
Identity = 33/67 (49.25%), Postives = 46/67 (68.66%), Query Frame = 0

Query: 8   LLGVPDEVIKRAASVLDAIGNNKHVERLHNENLSAHDKLYQDAVDKLLGLDVNKCDLSLF 67
           L GVP EV++RA +VL  I + + + R+  E L+A D+ YQDAV KLL  D +K DL  F
Sbjct: 743 LAGVPSEVVQRAVTVLGDIHSKRPIRRMVWEKLAAKDQQYQDAVTKLLAFDPHKGDLVNF 802

Query: 68  FRDIFPS 75
           F+++FPS
Sbjct: 803 FQEVFPS 809

BLAST of Spg030521 vs. ExPASy TrEMBL
Match: A0A6J1L1D5 (DNA mismatch repair protein MSH5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500234 PE=4 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 7.5e-25
Identity = 60/67 (89.55%), Postives = 62/67 (92.54%), Query Frame = 0

Query: 8   LLGVPDEVIKRAASVLDAIGNNKHVERLHNENLSAHDKLYQDAVDKLLGLDVNKCDLSLF 67
           L GVPDEVIKRAA VLDA+GNNKHVERLHNENLSA DKLYQDAVDKLLGLDVNKCDLS F
Sbjct: 760 LAGVPDEVIKRAAFVLDAMGNNKHVERLHNENLSAQDKLYQDAVDKLLGLDVNKCDLSRF 819

Query: 68  FRDIFPS 75
           F+ IFPS
Sbjct: 820 FQGIFPS 826

BLAST of Spg030521 vs. ExPASy TrEMBL
Match: A0A6J1F1R7 (DNA mismatch repair protein MSH5 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111438624 PE=4 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 7.5e-25
Identity = 60/67 (89.55%), Postives = 62/67 (92.54%), Query Frame = 0

Query: 8   LLGVPDEVIKRAASVLDAIGNNKHVERLHNENLSAHDKLYQDAVDKLLGLDVNKCDLSLF 67
           L GVPDEVIKRAA VLDA+GNNKHVERLHNENLSA DKLYQDAVDKLLGLDVNKCDLS F
Sbjct: 742 LAGVPDEVIKRAAFVLDAMGNNKHVERLHNENLSAQDKLYQDAVDKLLGLDVNKCDLSRF 801

Query: 68  FRDIFPS 75
           F+ IFPS
Sbjct: 802 FQGIFPS 808

BLAST of Spg030521 vs. ExPASy TrEMBL
Match: A0A6J1L5R0 (DNA mismatch repair protein MSH5 isoform X7 OS=Cucurbita maxima OX=3661 GN=LOC111500234 PE=4 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 7.5e-25
Identity = 60/67 (89.55%), Postives = 62/67 (92.54%), Query Frame = 0

Query: 8   LLGVPDEVIKRAASVLDAIGNNKHVERLHNENLSAHDKLYQDAVDKLLGLDVNKCDLSLF 67
           L GVPDEVIKRAA VLDA+GNNKHVERLHNENLSA DKLYQDAVDKLLGLDVNKCDLS F
Sbjct: 680 LAGVPDEVIKRAAFVLDAMGNNKHVERLHNENLSAQDKLYQDAVDKLLGLDVNKCDLSRF 739

Query: 68  FRDIFPS 75
           F+ IFPS
Sbjct: 740 FQGIFPS 746

BLAST of Spg030521 vs. ExPASy TrEMBL
Match: A0A6J1L3Q2 (DNA mismatch repair protein MSH5 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111500234 PE=4 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 7.5e-25
Identity = 60/67 (89.55%), Postives = 62/67 (92.54%), Query Frame = 0

Query: 8   LLGVPDEVIKRAASVLDAIGNNKHVERLHNENLSAHDKLYQDAVDKLLGLDVNKCDLSLF 67
           L GVPDEVIKRAA VLDA+GNNKHVERLHNENLSA DKLYQDAVDKLLGLDVNKCDLS F
Sbjct: 742 LAGVPDEVIKRAAFVLDAMGNNKHVERLHNENLSAQDKLYQDAVDKLLGLDVNKCDLSRF 801

Query: 68  FRDIFPS 75
           F+ IFPS
Sbjct: 802 FQGIFPS 808

BLAST of Spg030521 vs. ExPASy TrEMBL
Match: A0A6J1L8E4 (DNA mismatch repair protein MSH5 isoform X6 OS=Cucurbita maxima OX=3661 GN=LOC111500234 PE=4 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 7.5e-25
Identity = 60/67 (89.55%), Postives = 62/67 (92.54%), Query Frame = 0

Query: 8   LLGVPDEVIKRAASVLDAIGNNKHVERLHNENLSAHDKLYQDAVDKLLGLDVNKCDLSLF 67
           L GVPDEVIKRAA VLDA+GNNKHVERLHNENLSA DKLYQDAVDKLLGLDVNKCDLS F
Sbjct: 696 LAGVPDEVIKRAAFVLDAMGNNKHVERLHNENLSAQDKLYQDAVDKLLGLDVNKCDLSRF 755

Query: 68  FRDIFPS 75
           F+ IFPS
Sbjct: 756 FQGIFPS 762

BLAST of Spg030521 vs. TAIR 10
Match: AT3G20475.1 (MUTS-homologue 5 )

HSP 1 Score: 73.2 bits (178), Expect = 1.0e-13
Identity = 34/67 (50.75%), Postives = 50/67 (74.63%), Query Frame = 0

Query: 8   LLGVPDEVIKRAASVLDAIGNNKHVERLHNENLSAHDKLYQDAVDKLLGLDVNKCDLSLF 67
           L GVP+EV+KRAA VLDA  +N +V++L  + +S+ D+ ++DAVDK   LD++K D+  F
Sbjct: 741 LAGVPEEVVKRAAIVLDAFESNNNVDKLSLDKISSQDQAFKDAVDKFAELDISKGDIHAF 800

Query: 68  FRDIFPS 75
           F+DIF S
Sbjct: 801 FQDIFTS 807

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023553301.11.6e-2489.55DNA mismatch repair protein MSH5 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023... [more]
XP_023007704.11.6e-2489.55DNA mismatch repair protein MSH5 isoform X7 [Cucurbita maxima][more]
XP_023007699.11.6e-2489.55DNA mismatch repair protein MSH5 isoform X2 [Cucurbita maxima][more]
XP_023007696.11.6e-2489.55DNA mismatch repair protein MSH5 isoform X1 [Cucurbita maxima] >XP_023007698.1 D... [more]
XP_022932275.11.6e-2489.55DNA mismatch repair protein MSH5 isoform X6 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
F4JEP51.4e-1250.75DNA mismatch repair protein MSH5 OS=Arabidopsis thaliana OX=3702 GN=MSH5 PE=2 SV... [more]
Q6L4V03.0e-1049.25DNA mismatch repair protein MSH5 OS=Oryza sativa subsp. japonica OX=39947 GN=MSH... [more]
Match NameE-valueIdentityDescription
A0A6J1L1D57.5e-2589.55DNA mismatch repair protein MSH5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1F1R77.5e-2589.55DNA mismatch repair protein MSH5 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1L5R07.5e-2589.55DNA mismatch repair protein MSH5 isoform X7 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1L3Q27.5e-2589.55DNA mismatch repair protein MSH5 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1L8E47.5e-2589.55DNA mismatch repair protein MSH5 isoform X6 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT3G20475.11.0e-1350.75MUTS-homologue 5 [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg030521.1Spg030521.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006298 mismatch repair
molecular_function GO:0005524 ATP binding
molecular_function GO:0030983 mismatched DNA binding