Spg030489 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg030489
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionReverse transcriptase domain-containing protein
Locationscaffold6: 33678414 .. 33681054 (-)
RNA-Seq ExpressionSpg030489
SyntenySpg030489
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAATTGATTAATGCCAAAATGGGGTGCCAATGTAAACCCAGGACTTCTCTAGGATCATTTTCCCTTGTCATCTATTGTTGGTTTAAATTGTTGGGATTACTAAGTTAAAAGATGAAAGTAAAGAAAAGGAAAAGGAAAAGAAAAATGGAAGGAAAAGAAAAAAAAAAACAGAACAAATGTGAAAGAAAAAAAAAGAGAAAAGAAGGAAGTGGGAATGGACAGCAAGGAAACCCACCACCGACAACCACTCTATTCAGCCCATTTTCATTCCATTCCTCATCCTTTTCCATTAAAACTTTCAGAGCACTTTAAGAAGAGAGTGAAGGAGGAGGAAGAAGAAGAATTTCGTGGTTTGAGGTTGAAGAAGATAGGGAAAAGCTCATGCAAAGCCAGCCATTGCAGAATCTGGGTTCAACCTGTCAACCTCTGGTGTTTTAGTCGGTTTGGGCTACACAGAAGGAATCAAGAGGTGAGGTTTGGTTTTCTTGAAAGTTAGTTCATTTTCTAACAAACTTCATGAAGGAAGCTTGACCTAAATCGAATGAGAAGTTGATGGTTTTTGGATTGGAAAACAGAGCACAGGATTTTCGGGCAAACCAGGAGGATCTCTGGTGTTCTCTGATGTTTTGAGTATTGTGGAGTGCACAAGTCGAATCCGAAGCTCTATCTGGTATGGTTAGAAAGGGTATTCAATATCCTACAACATTTGTGAAGATTCCGTGAGCCAAAAATGACTGTATTTAGGTTTAATTTAAGGAGAAAGATAAGGGGTAACAGAGAAGCTCGAATCTGTGTTGTCTGATTTCGGGGCATTTCTGGACAAATTGGTTGGGCATCTCGTTTTCATTTCTTCAGGTAAGTTCTAGAGTGTTCGAAAATGAAGAGTTTGATGTATATTTCATTAATTTTGGTTAGTTATTGAGGAAGTTATGGGTGTTGGAAGTTAGGGTATAGAATCTGGAAAATCTGGAAAGTAGAATGTGAATGTGATTCTTAATCCAATTGTTTAGAAAATTCATGAGCATGGAAAGACAAAGCTATCTATGGTTCTAATGCTCGGTTTTCGTTATTTGACAGGCCAAGAGGAAATAGGCCGAGTCTACAAACCGGGGAACTAGATAAGACAGTGAGTGACAAAACAAATTTTGAAAATGTTCTTAAACGGTTGTTCATGATTATATGTTTTACATGCTTTTCTATGAATGATACATGGTTTTTGTGAACGGTTTCAAGCATGAGTTTTGAGCTCAGATTTTCTAATGAGGTTTATATACGAGCACGTGGCTAATGGCTTTATGAAAAGTAGTTGAAACGATATTTTTTTTAAAGTGAAGCATTGATAGCGAGCATAGCTAGCTATCTTTAATTATTTAATTTAGCTAGTCTTTTATGATTTTTGGGCCGTTTTATTGTGCTTTAATGTTTATTTTGTAGGAAAAGGTTGACTGGAGCTAAAATGAAAAAGTGGAGTTAATCGGGTGCTCGGGATGCGAAAAGATGCAAAAGAAGGAAAAGAATCAAAAGGGAAAAAAGTCAAAATTCGGTCAAAAGGTGACTAGCGTCTCGACGCTAGCATTCCTTATTCGGATAGGCGCGAAAACGTCGCAGCGTCGAGACGCTGCGACCTTAGCGTCTCGACATGTGTTAGCAAATATTGTTTAATGATTTAAAGTTTTTCACGAGCAAGCTGAGCAAGCTTGAGTTTTACGAAAGATAGCGATAAGCAGGCATGTTTAAATGTTTTCATGTGATTTTCTGAGATTTCTATTGACTACATGACTGAGATATTGAGCCCGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCCGTTGTCGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGCGGGCCCCACTACATCGTAGGACTAGAAACGTTGGTTGTACTGGGCTTGCCCTACACAACGTAGATCGGTCATGTTAGTTAAAACGTTTGATGTATTTCATATGCCTTGCTAGATTTTTATTGACGAATTTGCGAGATATTTTAGAAAGCCTTTTCCATTACACGTCTATGTTAAACGCTTTGATTGAGCATATTTATATGTTTAAAGGTTTATCACATGTTTTTGACTTCCGGATTTAGAGTTTTAAGATAGTCACTCACTGGGCTTCGTAGCTAATTCTTTATAAATGTTTTCCACTTTTCAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCCGGATCAAGCTTCAGTACGTGGGTGGAGTTGTAAATTGAGTCTGTTCATATTGTAAATGTTTTGTAAGGATTGGATAATATAGTCCTAGAGGTCGTGGTTGTATAAATCCTAGTTGTACATGTCTTTGGTTATGATATCTATATCAGGGTATTTCCAAACCGAGATATGTCATGGCGACACCTCGTGTATGTTGATATGTTTTCGGGGTTTCCGTTGTGTTGTGGTTCATATGGAATATTTTATATCCATGATTAGTCTGTTTATCAGGTTCAACGTTTCACTAGGGTCAACAGGGATCGTTAGAGGTGACGATGTCTGTTGGCTTCAAGTCGTCTTTCGGGCTAAGCTAGCAGGTGGTCCGGGAGGGGGTGTGACAGCCAAGGGGTGCCTTTTATAG

mRNA sequence

ATGGACAGCAAGGAAACCCACCACCGACAACCACTCTATTCAGCCCATTTTCATTCCATTCCTCATCCTTTTCCATTAAAACTTTCAGAGCACTTTAAGAAGAGAGTGAAGGAGGAGGAAGAAGAAGAATTTCGTGGTTTGAGGTTGAAGAAGATAGGGAAAAGCTCATGCAAAGCCAGCCATTGCAGAATCTGGGTTCAACCTGTCAACCTCTGGTGTTTTAGTCGGTTTGGGCTACACAGAAGGAATCAAGAGAGCACAGGATTTTCGGGCAAACCAGGAGGATCTCTGGTGTTCTCTGATGTTTTGAGTATTGTGGAGTGCACAAGTCGAATCCGAAGCTCTATCTGGCCAAGAGGAAATAGGCCGAGTCTACAAACCGGGGAACTAGATAAGACAGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCCGGATCAAGCTTCAGGTCAACAGGGATCGTTAGAGGTGACGATGTCTGTTGGCTTCAAGTCGTCTTTCGGGCTAAGCTAGCAGGTGGTCCGGGAGGGGGTGTGACAGCCAAGGGGTGCCTTTTATAG

Coding sequence (CDS)

ATGGACAGCAAGGAAACCCACCACCGACAACCACTCTATTCAGCCCATTTTCATTCCATTCCTCATCCTTTTCCATTAAAACTTTCAGAGCACTTTAAGAAGAGAGTGAAGGAGGAGGAAGAAGAAGAATTTCGTGGTTTGAGGTTGAAGAAGATAGGGAAAAGCTCATGCAAAGCCAGCCATTGCAGAATCTGGGTTCAACCTGTCAACCTCTGGTGTTTTAGTCGGTTTGGGCTACACAGAAGGAATCAAGAGAGCACAGGATTTTCGGGCAAACCAGGAGGATCTCTGGTGTTCTCTGATGTTTTGAGTATTGTGGAGTGCACAAGTCGAATCCGAAGCTCTATCTGGCCAAGAGGAAATAGGCCGAGTCTACAAACCGGGGAACTAGATAAGACAGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCCGGATCAAGCTTCAGGTCAACAGGGATCGTTAGAGGTGACGATGTCTGTTGGCTTCAAGTCGTCTTTCGGGCTAAGCTAGCAGGTGGTCCGGGAGGGGGTGTGACAGCCAAGGGGTGCCTTTTATAG

Protein sequence

MDSKETHHRQPLYSAHFHSIPHPFPLKLSEHFKKRVKEEEEEEFRGLRLKKIGKSSCKASHCRIWVQPVNLWCFSRFGLHRRNQESTGFSGKPGGSLVFSDVLSIVECTSRIRSSIWPRGNRPSLQTGELDKTVEIELPVPDTLPTSAESSGSSFRSTGIVRGDDVCWLQVVFRAKLAGGPGGGVTAKGCLL
Homology
BLAST of Spg030489 vs. NCBI nr
Match: TYK08801.1 (hypothetical protein E5676_scaffold796G00040 [Cucumis melo var. makuwa])

HSP 1 Score: 155.2 bits (391), Expect = 5.6e-34
Identity = 116/302 (38.41%), Postives = 130/302 (43.05%), Query Frame = 0

Query: 4   KETHHRQPLYSAHFHSIPHPFPLKLSEHFKKRVKEEEEEEFRGLRLKKIGKSSCKASHCR 63
           K  H +  L+  +F S  H FP KLSEHF++RVK EEEEEFRGLRLKKIGKSSCKA+HCR
Sbjct: 48  KVHHCKLILFQPNFISFLHHFPSKLSEHFEERVK-EEEEEFRGLRLKKIGKSSCKANHCR 107

Query: 64  IWVQPVNLWCFSRFGLHRRNQESTGFSGKPGGSLVFSDVLSIVECTSRIRSSIW------ 123
           IW                    STGFS KP G LVFSD LSI+E     +   W      
Sbjct: 108 IW--------------------STGFSSKPRGFLVFSDALSILEVQKSNQKLYWDIESGK 167

Query: 124 -------PRGNRPSLQTGELDK-------------------------------------- 183
                  PRGNR SLQTG+LDK                                      
Sbjct: 168 SGKWDVMPRGNRSSLQTGQLDKIEISIDYMTEILSLRLYGTVCPQVIFPLSTLSVLRDND 227

Query: 184 -----------------------------------------TVEIELPVPDTLPTSAESS 187
                                                    ++EIEL VPDTLPTSAESS
Sbjct: 228 AVVSVGQTHYDKDDGVPGGPHYIVGLVNVGCTGRVLHHIDRSLEIELLVPDTLPTSAESS 287

BLAST of Spg030489 vs. NCBI nr
Match: KAA0060199.1 (uncharacterized protein E6C27_scaffold386G00120 [Cucumis melo var. makuwa] >TYJ95571.1 uncharacterized protein E5676_scaffold767G00770 [Cucumis melo var. makuwa])

HSP 1 Score: 145.2 bits (365), Expect = 5.8e-31
Identity = 86/143 (60.14%), Postives = 93/143 (65.03%), Query Frame = 0

Query: 69  VNLWCFSRFGLHRRNQESTGFSGKPGGSLVFSDVLSIVEC--TSRIRSSIWPRGNRPSLQ 128
           VNLW F           ST FS KPGG LVFSD LSI+E   +++    + PRGNRPSLQ
Sbjct: 13  VNLWLFC---------SSTRFSSKPGGFLVFSDALSILEMHKSNQKLYLVRPRGNRPSLQ 72

Query: 129 TGELDKT-----------------VEIELPVPDTLPTSAESSGSSFRSTGIVRGDDVCWL 188
           TG+LDKT                 VEIELPVPDTLPTSAES  S+ RSTGIVRGDDVCWL
Sbjct: 73  TGKLDKTVIFSLSTLSVLRDNDAVVEIELPVPDTLPTSAESFESNSRSTGIVRGDDVCWL 132

Query: 189 QVVFRAKLAGGPGGGVT---AKG 190
             VFRAK AGGPGGGVT   AKG
Sbjct: 133 HAVFRAKTAGGPGGGVTFNIAKG 146

BLAST of Spg030489 vs. NCBI nr
Match: KAA0064296.1 (putative DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa] >TYK01032.1 putative DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 129.8 bits (325), Expect = 2.5e-26
Identity = 86/164 (52.44%), Postives = 93/164 (56.71%), Query Frame = 0

Query: 69  VNLWCFSRFGL-HRRNQESTGFSGKPGGSLVFSDVLSIVECTSRIR---------SSIWP 128
           VNLW F  FGL ++   +STGFS KPG  LVFSD LSI++     +          S  P
Sbjct: 497 VNLW-FVLFGLSYQEGTKSTGFSSKPGRFLVFSDALSILKVPKSNQKLYLDIESGKSGKP 556

Query: 129 RGNRPSLQTGELDKT-----------------VEIELPVPDTLPTSAESSGS-------- 187
           RGN+PSLQTGELDKT                 VEIEL VPDTLPTSAES GS        
Sbjct: 557 RGNKPSLQTGELDKTVIFPLSTFSVLHDNDAVVEIELSVPDTLPTSAESYGSNSSYFTNQ 616

BLAST of Spg030489 vs. NCBI nr
Match: TYK11835.1 (uncharacterized protein E5676_scaffold152G00520 [Cucumis melo var. makuwa])

HSP 1 Score: 128.6 bits (322), Expect = 5.6e-26
Identity = 85/171 (49.71%), Postives = 92/171 (53.80%), Query Frame = 0

Query: 69  VNLWCFSRFGL-HRRNQESTGFSGKPGGSLVFSDVLSIVECTSRIRSSIW--PRGNRPSL 128
           VNLW F  F L +    +STGFS KPGG LV SD LSI+E   ++   ++  PRGNR SL
Sbjct: 13  VNLW-FVLFSLSYPEGTKSTGFSSKPGGFLVLSDALSILE-IHKLNQKLYLGPRGNRLSL 72

Query: 129 QTGELDKTV---------------EIELPVPDTLPTSAESSGS----------------- 187
           QTGELDKTV                IELPVPDTLPTSAESSGS                 
Sbjct: 73  QTGELDKTVIFSLSTLSVLRDNNAVIELPVPDTLPTSAESSGSNSSTWLELYFESVHVEM 132

BLAST of Spg030489 vs. NCBI nr
Match: TYK25935.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 127.1 bits (318), Expect = 1.6e-25
Identity = 81/165 (49.09%), Postives = 87/165 (52.73%), Query Frame = 0

Query: 81  RRNQE----STGFSGKPGGSLVFSDVLSIVE---CTSRIRSSI---WPRGNRPSLQTGEL 140
           RRN+     STGFS KPGG LVFSD LSI E      ++   I   WPRGNRP LQTGEL
Sbjct: 256 RRNRTLLDMSTGFSSKPGGFLVFSDALSIQEVHKSNQKLYLEIDKEWPRGNRPGLQTGEL 315

Query: 141 DK-----------------TVEIELPVPDTLPTSAESSGSSFR----------------- 187
           DK                  VEIELP+PDTLPTSAESSGS+                   
Sbjct: 316 DKIVIFPLSTLSVLRDNDAVVEIELPLPDTLPTSAESSGSNSSTWLELYFESVHVEMLFY 375

BLAST of Spg030489 vs. ExPASy TrEMBL
Match: A0A5D3CAC6 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold796G00040 PE=4 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 2.7e-34
Identity = 116/302 (38.41%), Postives = 130/302 (43.05%), Query Frame = 0

Query: 4   KETHHRQPLYSAHFHSIPHPFPLKLSEHFKKRVKEEEEEEFRGLRLKKIGKSSCKASHCR 63
           K  H +  L+  +F S  H FP KLSEHF++RVK EEEEEFRGLRLKKIGKSSCKA+HCR
Sbjct: 48  KVHHCKLILFQPNFISFLHHFPSKLSEHFEERVK-EEEEEFRGLRLKKIGKSSCKANHCR 107

Query: 64  IWVQPVNLWCFSRFGLHRRNQESTGFSGKPGGSLVFSDVLSIVECTSRIRSSIW------ 123
           IW                    STGFS KP G LVFSD LSI+E     +   W      
Sbjct: 108 IW--------------------STGFSSKPRGFLVFSDALSILEVQKSNQKLYWDIESGK 167

Query: 124 -------PRGNRPSLQTGELDK-------------------------------------- 183
                  PRGNR SLQTG+LDK                                      
Sbjct: 168 SGKWDVMPRGNRSSLQTGQLDKIEISIDYMTEILSLRLYGTVCPQVIFPLSTLSVLRDND 227

Query: 184 -----------------------------------------TVEIELPVPDTLPTSAESS 187
                                                    ++EIEL VPDTLPTSAESS
Sbjct: 228 AVVSVGQTHYDKDDGVPGGPHYIVGLVNVGCTGRVLHHIDRSLEIELLVPDTLPTSAESS 287

BLAST of Spg030489 vs. ExPASy TrEMBL
Match: A0A5D3BAD1 (Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold767G00770 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 2.8e-31
Identity = 86/143 (60.14%), Postives = 93/143 (65.03%), Query Frame = 0

Query: 69  VNLWCFSRFGLHRRNQESTGFSGKPGGSLVFSDVLSIVEC--TSRIRSSIWPRGNRPSLQ 128
           VNLW F           ST FS KPGG LVFSD LSI+E   +++    + PRGNRPSLQ
Sbjct: 13  VNLWLFC---------SSTRFSSKPGGFLVFSDALSILEMHKSNQKLYLVRPRGNRPSLQ 72

Query: 129 TGELDKT-----------------VEIELPVPDTLPTSAESSGSSFRSTGIVRGDDVCWL 188
           TG+LDKT                 VEIELPVPDTLPTSAES  S+ RSTGIVRGDDVCWL
Sbjct: 73  TGKLDKTVIFSLSTLSVLRDNDAVVEIELPVPDTLPTSAESFESNSRSTGIVRGDDVCWL 132

Query: 189 QVVFRAKLAGGPGGGVT---AKG 190
             VFRAK AGGPGGGVT   AKG
Sbjct: 133 HAVFRAKTAGGPGGGVTFNIAKG 146

BLAST of Spg030489 vs. ExPASy TrEMBL
Match: A0A5A7VD33 (Putative DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold264G00370 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 1.2e-26
Identity = 86/164 (52.44%), Postives = 93/164 (56.71%), Query Frame = 0

Query: 69  VNLWCFSRFGL-HRRNQESTGFSGKPGGSLVFSDVLSIVECTSRIR---------SSIWP 128
           VNLW F  FGL ++   +STGFS KPG  LVFSD LSI++     +          S  P
Sbjct: 497 VNLW-FVLFGLSYQEGTKSTGFSSKPGRFLVFSDALSILKVPKSNQKLYLDIESGKSGKP 556

Query: 129 RGNRPSLQTGELDKT-----------------VEIELPVPDTLPTSAESSGS-------- 187
           RGN+PSLQTGELDKT                 VEIEL VPDTLPTSAES GS        
Sbjct: 557 RGNKPSLQTGELDKTVIFPLSTFSVLHDNDAVVEIELSVPDTLPTSAESYGSNSSYFTNQ 616

BLAST of Spg030489 vs. ExPASy TrEMBL
Match: A0A5D3CJX3 (CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00520 PE=4 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 2.7e-26
Identity = 85/171 (49.71%), Postives = 92/171 (53.80%), Query Frame = 0

Query: 69  VNLWCFSRFGL-HRRNQESTGFSGKPGGSLVFSDVLSIVECTSRIRSSIW--PRGNRPSL 128
           VNLW F  F L +    +STGFS KPGG LV SD LSI+E   ++   ++  PRGNR SL
Sbjct: 13  VNLW-FVLFSLSYPEGTKSTGFSSKPGGFLVLSDALSILE-IHKLNQKLYLGPRGNRLSL 72

Query: 129 QTGELDKTV---------------EIELPVPDTLPTSAESSGS----------------- 187
           QTGELDKTV                IELPVPDTLPTSAESSGS                 
Sbjct: 73  QTGELDKTVIFSLSTLSVLRDNNAVIELPVPDTLPTSAESSGSNSSTWLELYFESVHVEM 132

BLAST of Spg030489 vs. ExPASy TrEMBL
Match: A0A5A7T9P7 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1164G00060 PE=4 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 7.9e-26
Identity = 81/165 (49.09%), Postives = 87/165 (52.73%), Query Frame = 0

Query: 81  RRNQE----STGFSGKPGGSLVFSDVLSIVE---CTSRIRSSI---WPRGNRPSLQTGEL 140
           RRN+     STGFS KPGG LVFSD LSI E      ++   I   WPRGNRP LQTGEL
Sbjct: 256 RRNRTLLDMSTGFSSKPGGFLVFSDALSIQEVHKSNQKLYLEIDKEWPRGNRPGLQTGEL 315

Query: 141 DK-----------------TVEIELPVPDTLPTSAESSGSSFR----------------- 187
           DK                  VEIELP+PDTLPTSAESSGS+                   
Sbjct: 316 DKIVIFPLSTLSVLRDNDAVVEIELPLPDTLPTSAESSGSNSSTWLELYFESVHVEMLFY 375

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK08801.15.6e-3438.41hypothetical protein E5676_scaffold796G00040 [Cucumis melo var. makuwa][more]
KAA0060199.15.8e-3160.14uncharacterized protein E6C27_scaffold386G00120 [Cucumis melo var. makuwa] >TYJ9... [more]
KAA0064296.12.5e-2652.44putative DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa] >TYK... [more]
TYK11835.15.6e-2649.71uncharacterized protein E5676_scaffold152G00520 [Cucumis melo var. makuwa][more]
TYK25935.11.6e-2549.09gag/pol protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3CAC62.7e-3438.41Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5D3BAD12.8e-3160.14Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5A7VD331.2e-2652.44Putative DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=... [more]
A0A5D3CJX32.7e-2649.71CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A5A7T9P77.9e-2649.09Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1164G000... [more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg030489.1Spg030489.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043170 macromolecule metabolic process
biological_process GO:0006807 nitrogen compound metabolic process
biological_process GO:0044238 primary metabolic process
molecular_function GO:0003824 catalytic activity