Spg030358 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg030358
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Reverse transcriptase
Location	scaffold6: 25368045 .. 25369769 (+)
RNA-Seq Expression	Spg030358
Synteny	Spg030358

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTGAGATATTGAGCCCGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGCGGGCCCCACTACGACAAAGACGATGGGAGTGCTGGGCGGGCCCCACTACATCGTAGGACTAGTAAACGTTGGTTGTACTGGGCGTGCCCTACACAACGTAGATCGGTCATGTTAGTTAAAACGTTTGATGTACTTGATATGCCTTGCTAGATTTTTAATGACGAACTTGCGAGATATTTTAGAAAGCCTTTTTCATTACACGTTTATGTTAAACGCTTTGATTGAGCATATTTATTCGTTTAAAGGTTTATCACGTGTTTGACTTCCGGATTTAGAGTTTTAAAGTTAGTCACTCACTGGGCTTCGTAGCTCATCCGTTCAAAATGTTTTTCCACTTTCAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCTAGATCAAGCTCCAGTACGTGGTTGGAGTTGTATATTGAGTCCGTTCATATTGTAAATGTTTTGTAAGGGTTGTATAATATAGTCCTAGAGGTCGTGGATGTATAACCCTAGTTGTATATGTTTGGTCGTGATATCTATATCAGGTTACTTTCCAAACCAGGTTATGTCAGGGCTGCACCATGTGTATGTTGAAAGGTTTCCGTGGATTCCGCTGTGTGGTGTTTCGTATAGAATGTTTTATACCTATGATTAGTATGTTTATCAGGTTCAGCGTTTCAATAGGGTCAACAGGGATCGTTAGAGGTGACGATGTCTGTTGGCTTCACGCCGTCTTTCGGGCTAACCTAGCAGGTGGTCCGGGAGGGGGTGTGACAATCTTCAATTTCATAAATCTAGAAGTAGTTATAAGCGGATTTCTCTTTCAAGCTCCTCCACATCTCATACTAACATAACTAATTCATGTGTCCATCTCCTTAGTTAATCATAGTATGGCATTAGGCAACCTCATAATCAATTTCTAACAATTCTCTTTCACTCTCGTGTTCCAAGCAATTATTAGCCATGCAATTTGGATCTAATCTAAATTCTCTCTGTCGAGTATCAATTTAGACCAACATAACAATTAATCCGTGATCAAGAGATTAAAAGCATTAAGAATGAATTGAATAGATGAAATATGAAGAAATTCATTACATAATCCAAGTAATTGCGCTACAATATCCAATTAGCTAGAGTAACGACCCTAGGTCTCAAGATCTACTCACAATTGGAAATAATTTTGCCAAAATGATCTTGATCCATGGGCCTAACTCAAATTACAATAAGAAATTATAAAGAAGTAAGGAAGAAATATCCGATAATTTGATCCCAAGCTCCCGCTACGAACATCTCGAATCCGAGCCTTGGAATCTCGCTCAATTCCTCCTTTTTCCTCCCAACTCTTCTCTCTTTTACTCTCTATTGAGCTAGGGGAGAGCTCCCCAAAATGGGATGATTTGAACCTTGGGAGAGGGGGCTATTTATAGGAGCAAGGGCTGGACATTTCTTGCTGAACAAGTCGCCAGCGTCGAGACGCTACGTCACAGCGTCGAGACGCCGTGACGTCAATTTTCTTAGAGCCGAAGTTGTCGCAGCGTCGCGACGCTGTCGCGATTCTGGATTTTTCCTCGCTGATCACCTAGCGTCGAGACACTCCAGTCCTAGCGTCGAGACGCTCTAG

mRNA sequence

ATGACTGAGATATTGAGCCCGAGGCTATATGGTACCGTGTGCACACAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCTAGATCAAGCTCCAGTACGTGGTTGGAGTTGTATATTGAGTCCGTTACTTTCCAAACCAGGTTATGTCAGGGCTGCACCATGTGTATGTTGAAAGGTTTCCGTGGATTCCGCTGTGTGGTGTTTCGTATAGAATGTTTTATACCTATGATTAGTATGTTTATCAGGTTCAGCGTTTCAATAGGGTCAACAGGGATCGTTAGAGGTGACGATGTCTGTTGGCTTCACGCCGTCTTTCGGGCTAACCTAGCAGGTGGTCCGGGAGGGGGAGCAAGGGCTGGACATTTCTTGCTGAACAAGTCGCCAGCGTCGAGACGCTACGTCACAGCGTCGAGACGCCGTGACGTCAATTTTCTTAGAGCCGAAGTTGTCGCAGCGTCGCGACGCTGTCGCGATTCTGGATTTTTCCTCGCTGATCACCTAGCGTCGAGACACTCCAGTCCTAGCGTCGAGACGCTCTAG

Coding sequence (CDS)

ATGACTGAGATATTGAGCCCGAGGCTATATGGTACCGTGTGCACACAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCTAGATCAAGCTCCAGTACGTGGTTGGAGTTGTATATTGAGTCCGTTACTTTCCAAACCAGGTTATGTCAGGGCTGCACCATGTGTATGTTGAAAGGTTTCCGTGGATTCCGCTGTGTGGTGTTTCGTATAGAATGTTTTATACCTATGATTAGTATGTTTATCAGGTTCAGCGTTTCAATAGGGTCAACAGGGATCGTTAGAGGTGACGATGTCTGTTGGCTTCACGCCGTCTTTCGGGCTAACCTAGCAGGTGGTCCGGGAGGGGGAGCAAGGGCTGGACATTTCTTGCTGAACAAGTCGCCAGCGTCGAGACGCTACGTCACAGCGTCGAGACGCCGTGACGTCAATTTTCTTAGAGCCGAAGTTGTCGCAGCGTCGCGACGCTGTCGCGATTCTGGATTTTTCCTCGCTGATCACCTAGCGTCGAGACACTCCAGTCCTAGCGTCGAGACGCTCTAG

Protein sequence

MTEILSPRLYGTVCTQVEIELPVPDTLPTSAESSRSSSSTWLELYIESVTFQTRLCQGCTMCMLKGFRGFRCVVFRIECFIPMISMFIRFSVSIGSTGIVRGDDVCWLHAVFRANLAGGPGGGARAGHFLLNKSPASRRYVTASRRRDVNFLRAEVVAASRRCRDSGFFLADHLASRHSSPSVETL

Homology

[Close]

BLAST of Spg030358 vs. NCBI nr
Match: KAA0054353.1 (uncharacterized protein E6C27_scaffold24G00800 [Cucumis melo var. makuwa] >TYK12390.1 uncharacterized protein E5676_scaffold302G001720 [Cucumis melo var. makuwa])

HSP 1 Score: 124.4 bits (311), Expect = 1.0e-24
Identity = 69/113 (61.06%), Postives = 81/113 (71.68%), Query Frame = 0


Query: 17  VEIELPVPDTLPTSAESSRSSSSTWLELYIESVTFQ------TRLCQGCTMCMLKGFRGF 76
           VEIELPV DTL TSAESS S+SSTWL+LY ES+  +      + +     +C  +GFRGF
Sbjct: 97  VEIELPVLDTLATSAESSGSNSSTWLKLYFESLHVEMLFYKPSDVMTTSRVCW-EGFRGF 156

Query: 77  RCVVFRIECFIPMISMFIRFSVSIGSTGIVRGDDVCWLHAVFRANLAGGPGGG 124
           RCVVF +E  I  I MFIRF+VS+GSTGIVRG+DV W H VFRA  AGG GGG
Sbjct: 157 RCVVFHVEYSISKIGMFIRFNVSVGSTGIVRGEDVRWFHVVFRAKTAGGSGGG 208

[Close]

BLAST of Spg030358 vs. NCBI nr
Match: KAA0062407.1 (uncharacterized protein E6C27_scaffold154G002090 [Cucumis melo var. makuwa] >TYK26574.1 uncharacterized protein E5676_scaffold313G001830 [Cucumis melo var. makuwa])

HSP 1 Score: 121.3 bits (303), Expect = 8.7e-24
Identity = 69/124 (55.65%), Postives = 79/124 (63.71%), Query Frame = 0


Query: 1   MTEILSPRLYGTVCTQVEIELPVPDTLPTSAESSRSSSSTWLELYIESVTFQTRLCQGCT 60
           MTEILS RLYGTVCTQV+IELP+PDTLPTSAESS S+ +TWLELY ESV  +        
Sbjct: 1   MTEILSLRLYGTVCTQVKIELPMPDTLPTSAESSESNFNTWLELYFESVHVE-------- 60

Query: 61  MCMLKGFRGFRCVVFRIECFIP---MISMFIRFSVSIGSTGIVRGDDVCWLHAVFRANLA 120
                           I C+     M  + + F+VS+GSTGIVRGDDVCWLHAVFR   A
Sbjct: 61  ----------------IFCYFTNQVMSGLHLVFNVSVGSTGIVRGDDVCWLHAVFRDKTA 100

Query: 121 GGPG 122
             PG
Sbjct: 121 VDPG 100

[Close]

BLAST of Spg030358 vs. NCBI nr
Match: TYK27998.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 110.5 bits (275), Expect = 1.5e-20
Identity = 70/140 (50.00%), Postives = 73/140 (52.14%), Query Frame = 0


Query: 1   MTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSRSSSSTWLE 60
           MTEILSPRLYGTVCTQ                 VEIELPVPDTLPTSAESS S+SSTWLE
Sbjct: 1   MTEILSPRLYGTVCTQVIFPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLE 60

Query: 61  LYIESVTFQTRLCQGCTMCMLKGFRGFRCVVFRIECFIPMISMFIRFSVSIGSTGIVRGD 120
           LY+E V                           +E F                 GIVR D
Sbjct: 61  LYVEFV--------------------------HVEMF--------------WLAGIVRED 100

Query: 121 DVCWLHAVFRANLAGGPGGG 124
           DVCWLHAVFRA  AGGPGGG
Sbjct: 121 DVCWLHAVFRAKTAGGPGGG 100

[Close]

BLAST of Spg030358 vs. NCBI nr
Match: KAA0066851.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 110.5 bits (275), Expect = 1.5e-20
Identity = 70/140 (50.00%), Postives = 73/140 (52.14%), Query Frame = 0


Query: 1   MTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSRSSSSTWLE 60
           MTEILSPRLYGTVCTQ                 VEIELPVPDTLPTSAESS S+SSTWLE
Sbjct: 1   MTEILSPRLYGTVCTQVIFPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLE 60

Query: 61  LYIESVTFQTRLCQGCTMCMLKGFRGFRCVVFRIECFIPMISMFIRFSVSIGSTGIVRGD 120
           LY+E V                           +E F                 GIVR D
Sbjct: 61  LYVEFV--------------------------HVEMF--------------WLAGIVRED 100

Query: 121 DVCWLHAVFRANLAGGPGGG 124
           DVCWLHAVFRA  AGGPGGG
Sbjct: 121 DVCWLHAVFRAKTAGGPGGG 100

[Close]

BLAST of Spg030358 vs. NCBI nr
Match: KAA0062409.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 107.8 bits (268), Expect = 9.9e-20
Identity = 61/107 (57.01%), Postives = 67/107 (62.62%), Query Frame = 0


Query: 17  VEIELPVPDTLPTSAESSRSSSSTWLELYIESVTFQTRLCQGCTMCMLKGFRGFRCVVFR 76
           VEIELPVPDTLPTSAESS S+SSTWLELY ESV  +   C      M +           
Sbjct: 94  VEIELPVPDTLPTSAESSESNSSTWLELYFESVHVE-MFCYFTNQVMSR----------- 153

Query: 77  IECFIPMISMFIRFSVSIGSTGIVRGDDVCWLHAVFRANLAGGPGGG 124
                    + + FSV +GSTGIVRGDDVCWLHAVFRA   GGPGGG
Sbjct: 154 ---------LHLVFSVLVGSTGIVRGDDVCWLHAVFRAKTVGGPGGG 179

[Close]

BLAST of Spg030358 vs. ExPASy TrEMBL
Match: A0A5A7UEC1 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G001720 PE=4 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 5.0e-25
Identity = 69/113 (61.06%), Postives = 81/113 (71.68%), Query Frame = 0


Query: 17  VEIELPVPDTLPTSAESSRSSSSTWLELYIESVTFQ------TRLCQGCTMCMLKGFRGF 76
           VEIELPV DTL TSAESS S+SSTWL+LY ES+  +      + +     +C  +GFRGF
Sbjct: 97  VEIELPVLDTLATSAESSGSNSSTWLKLYFESLHVEMLFYKPSDVMTTSRVCW-EGFRGF 156

Query: 77  RCVVFRIECFIPMISMFIRFSVSIGSTGIVRGDDVCWLHAVFRANLAGGPGGG 124
           RCVVF +E  I  I MFIRF+VS+GSTGIVRG+DV W H VFRA  AGG GGG
Sbjct: 157 RCVVFHVEYSISKIGMFIRFNVSVGSTGIVRGEDVRWFHVVFRAKTAGGSGGG 208

[Close]

BLAST of Spg030358 vs. ExPASy TrEMBL
Match: A0A5A7V638 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G001830 PE=4 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 4.2e-24
Identity = 69/124 (55.65%), Postives = 79/124 (63.71%), Query Frame = 0


Query: 1   MTEILSPRLYGTVCTQVEIELPVPDTLPTSAESSRSSSSTWLELYIESVTFQTRLCQGCT 60
           MTEILS RLYGTVCTQV+IELP+PDTLPTSAESS S+ +TWLELY ESV  +        
Sbjct: 1   MTEILSLRLYGTVCTQVKIELPMPDTLPTSAESSESNFNTWLELYFESVHVE-------- 60

Query: 61  MCMLKGFRGFRCVVFRIECFIP---MISMFIRFSVSIGSTGIVRGDDVCWLHAVFRANLA 120
                           I C+     M  + + F+VS+GSTGIVRGDDVCWLHAVFR   A
Sbjct: 61  ----------------IFCYFTNQVMSGLHLVFNVSVGSTGIVRGDDVCWLHAVFRDKTA 100

Query: 121 GGPG 122
             PG
Sbjct: 121 VDPG 100

[Close]

BLAST of Spg030358 vs. ExPASy TrEMBL
Match: A0A5D3DX39 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G002140 PE=4 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 7.4e-21
Identity = 70/140 (50.00%), Postives = 73/140 (52.14%), Query Frame = 0


Query: 1   MTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSRSSSSTWLE 60
           MTEILSPRLYGTVCTQ                 VEIELPVPDTLPTSAESS S+SSTWLE
Sbjct: 1   MTEILSPRLYGTVCTQVIFPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLE 60

Query: 61  LYIESVTFQTRLCQGCTMCMLKGFRGFRCVVFRIECFIPMISMFIRFSVSIGSTGIVRGD 120
           LY+E V                           +E F                 GIVR D
Sbjct: 61  LYVEFV--------------------------HVEMF--------------WLAGIVRED 100

Query: 121 DVCWLHAVFRANLAGGPGGG 124
           DVCWLHAVFRA  AGGPGGG
Sbjct: 121 DVCWLHAVFRAKTAGGPGGG 100

[Close]

BLAST of Spg030358 vs. ExPASy TrEMBL
Match: A0A5A7VMG9 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold271G002220 PE=4 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 7.4e-21
Identity = 70/140 (50.00%), Postives = 73/140 (52.14%), Query Frame = 0


Query: 1   MTEILSPRLYGTVCTQ-----------------VEIELPVPDTLPTSAESSRSSSSTWLE 60
           MTEILSPRLYGTVCTQ                 VEIELPVPDTLPTSAESS S+SSTWLE
Sbjct: 1   MTEILSPRLYGTVCTQVIFPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSESNSSTWLE 60

Query: 61  LYIESVTFQTRLCQGCTMCMLKGFRGFRCVVFRIECFIPMISMFIRFSVSIGSTGIVRGD 120
           LY+E V                           +E F                 GIVR D
Sbjct: 61  LYVEFV--------------------------HVEMF--------------WLAGIVRED 100

Query: 121 DVCWLHAVFRANLAGGPGGG 124
           DVCWLHAVFRA  AGGPGGG
Sbjct: 121 DVCWLHAVFRAKTAGGPGGG 100

[Close]

BLAST of Spg030358 vs. ExPASy TrEMBL
Match: A0A5A7V9N3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold154G002110 PE=4 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 4.8e-20
Identity = 61/107 (57.01%), Postives = 67/107 (62.62%), Query Frame = 0


Query: 17  VEIELPVPDTLPTSAESSRSSSSTWLELYIESVTFQTRLCQGCTMCMLKGFRGFRCVVFR 76
           VEIELPVPDTLPTSAESS S+SSTWLELY ESV  +   C      M +           
Sbjct: 94  VEIELPVPDTLPTSAESSESNSSTWLELYFESVHVE-MFCYFTNQVMSR----------- 153

Query: 77  IECFIPMISMFIRFSVSIGSTGIVRGDDVCWLHAVFRANLAGGPGGG 124
                    + + FSV +GSTGIVRGDDVCWLHAVFRA   GGPGGG
Sbjct: 154 ---------LHLVFSVLVGSTGIVRGDDVCWLHAVFRAKTVGGPGGG 179

The following BLAST results are available for this feature:

BLAST of Spg030358 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0054353.1	1.0e-24	61.06	uncharacterized protein E6C27_scaffold24G00800 [Cucumis melo var. makuwa] >TYK12...	[more]
KAA0062407.1	8.7e-24	55.65	uncharacterized protein E6C27_scaffold154G002090 [Cucumis melo var. makuwa] >TYK...	[more]
TYK27998.1	1.5e-20	50.00	reverse transcriptase [Cucumis melo var. makuwa]	[more]
KAA0066851.1	1.5e-20	50.00	reverse transcriptase [Cucumis melo var. makuwa]	[more]
KAA0062409.1	9.9e-20	57.01	reverse transcriptase [Cucumis melo var. makuwa]	[more]

BLAST of Spg030358 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg030358 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UEC1	5.0e-25	61.06	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A5A7V638	4.2e-24	55.65	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A5D3DX39	7.4e-21	50.00	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold38...	[more]
A0A5A7VMG9	7.4e-21	50.00	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27...	[more]
A0A5A7V9N3	4.8e-20	57.01	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold15...	[more]

BLAST of Spg030358 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg030358.1	Spg030358.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043170	macromolecule metabolic process
biological_process	GO:0006807	nitrogen compound metabolic process
biological_process	GO:0044238	primary metabolic process
molecular_function	GO:0003824	catalytic activity