Spg030301 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg030301 vs. NCBI nr
Match: XP_008458669.1 (PREDICTED: uncharacterized protein LOC103497997 [Cucumis melo] >TYK24762.1 uncharacterized protein E5676_scaffold266G003250 [Cucumis melo var. makuwa])

HSP 1 Score: 621.3 bits (1601), Expect = 6.7e-174
Identity = 291/463 (62.85%), Postives = 358/463 (77.32%), Query Frame = 0

Query: 1   MAVPRFSAFKSSHNGKYLNCVPADDNTIPNYVQYGSDEIATTYTKFSIEAA-STGKDHVH 60
           M VPRF+ F+SS N KY+N V +DD  IPN+VQY SD +AT+ TKFS  A+  T K+  H
Sbjct: 1   MVVPRFAGFRSSKNQKYMNVVKSDDEKIPNFVQYSSDNLATSTTKFSFVASHDTAKNLYH 60

Query: 61  IRSIYNQKYLKVESTSEQWITATAVEANEEQSDWACTLFRIVYNSVTGAYQIIHVHYGKP 120
           I  IYNQKYL+ ES+S +WI ATA+E NE+QSDW CTLFRI + S    Y + H++YG+P
Sbjct: 61  IVCIYNQKYLRAESSSSRWICATALEPNEDQSDWGCTLFRITWGSTEQRYDLFHMYYGQP 120

Query: 121 VDLSNDLASRPNCLYIYTDSSSHENHAVLDWETMVLFPQHVVFKGDNGKYLKIHNQDYHD 180
           VDL ND +S PNCLYIY  SS+  NH+VLDWE M + PQHVVFKGDN KYLKIHNQD HD
Sbjct: 121 VDLKND-SSHPNCLYIYDQSSTIYNHSVLDWEVMSILPQHVVFKGDNNKYLKIHNQDSHD 180

Query: 181 FLRFYETDPHVSSVQQEVFVTSDGKLRIKGPNNKFWRAAPNWIWVDSTDETSNNSYTLFQ 240
           FLRF   DP  SSVQQEV +TSDGK+RIKGPNNKFWRA+PNWIW+DSTD +SNN  T+FQ
Sbjct: 181 FLRFLLDDPQDSSVQQEVVITSDGKIRIKGPNNKFWRASPNWIWIDSTDTSSNNQATVFQ 240

Query: 241 PYKINKNVVALRNLGMNYFCKRLTADWKEDCLDAIDSSMSKYTRMTVEEAILSRSVYNVQ 300
           P KI+ NV+ LRNLG N FC R +ADWKE CL A D +MS Y+R+TVEEA+LSR +YNVQ
Sbjct: 241 PCKISNNVICLRNLGNNMFCSRTSADWKESCLSANDHAMSNYSRLTVEEAVLSREIYNVQ 300

Query: 301 YRLSEGRIYGESIIAMDFQGAVNNSNETNTLSLEFFYTKEESQNWSAILAAELSYTMTMD 360
           YRLS+GRIYGE+I+A+DFQGA+N S+E N +   F Y +EESQ WS+ +  +L+Y+MT++
Sbjct: 301 YRLSDGRIYGENILAVDFQGAMNGSDEVNHMDFNFSYEEEESQCWSSTVGMKLAYSMTIE 360

Query: 361 AGIPLIVDAQIEISGQFSGSYTWGETHSTSEELGDTHEIEILPRTAVKVGLVATKGYCDV 420
           AGIPLI+D  I +SG+ S SYTWG+T+  S     ++ +E+ PRT+VKV  + TKGYCDV
Sbjct: 361 AGIPLIMDTNIVMSGEISESYTWGQTNKKSTPKRTSYSLEVQPRTSVKVSAMLTKGYCDV 420

Query: 421 PFSYTRRDVLVSGEVRITEHDDGLFTGANSYNFHYDAGDVKRL 463
           PFSYT+RD+LV G+ R TEHDDGLFTG N YNFHY + DV  L
Sbjct: 421 PFSYTQRDILVDGQTRTTEHDDGLFTGFNCYNFHYQSEDVTSL 462

BLAST of Spg030301 vs. NCBI nr
Match: XP_022156054.1 (uncharacterized protein LOC111023023 [Momordica charantia])

HSP 1 Score: 419.9 bits (1078), Expect = 3.0e-113
Identity = 212/462 (45.89%), Postives = 296/462 (64.07%), Query Frame = 0

Query: 3   VPRFSAFKSSHNGKYLNCVPADDNTIPNYVQYGSDEIATTYTKFSIEAASTGKDHVHIRS 62
           +P++   +S  +  YL  V  +D  I  YV+Y S ++ T YTK+ +E A  GK +V+IR 
Sbjct: 4   LPKYVGLRSLADDLYLRFV-KEDVQIHGYVKYDSHDVVTPYTKYEVEPAKVGKRYVNIRC 63

Query: 63  IYNQKYLKVESTSEQWITATAVEANEEQSDWACTLFRIVYNSVTGAYQIIHVHYGKPVDL 122
            Y+ KY    S + +++ A A E NE++S+W+CTLF  +++S   AY+I HV  G     
Sbjct: 64  CYSNKYWVRPSMNSRYVVAAADEPNEDESNWSCTLFEPIHDSGNQAYRIKHVQLGFNTCS 123

Query: 123 SNDLASRPNCLYIYTDSSSHEN----HAVLDWETMVLFPQHVVFKGDN-GKYLKIHNQDY 182
           S D +    CL+I T S+   N    H V+DWE+  +FP+HV FKG N GK+LK      
Sbjct: 124 SRDGSIHNECLFIMTSSAPPRNFGDLHIVMDWESFFIFPKHVAFKGGNRGKFLKAIWLQR 183

Query: 183 HDFLRFYETDPHVSSVQQEVFVTSDGKLRIKGPN-NKFWRAAPNWIWVDSTDETSNNSYT 242
           H++  F   D    +V  EV  T  G +RIK  +  KFWR +PNWIW DSTD T+NN +T
Sbjct: 184 HEYQEFSANDMADPAVGNEVSFTRHGDVRIKSNHFGKFWRLSPNWIWADSTDPTANNKHT 243

Query: 243 LFQPYKINKNVVALRNLGMNYFCKRLTADWKEDCLDAIDSSMSKYTRMTVEEAILSRSVY 302
           LF+P K+  N+VALRNLG N+FC  LT + K DCL+A  S+++   RM + E +LSRS+Y
Sbjct: 244 LFKPVKVANNIVALRNLGNNHFCIGLTTEGKTDCLNAWVSTITNEARMEIHELVLSRSIY 303

Query: 303 NVQYRLSEGRIYGESIIAMDFQGAVNNSNETNTLSLEFFYTKEESQNWSAILAAELSYTM 362
           NV + L + RIYGE+IIAM    AVNN+NE NT+++ F YT   +  WS+ L+A+L    
Sbjct: 304 NVDFHLFDARIYGENIIAMANGNAVNNTNEPNTITIRFSYTNRVTNTWSSTLSAKLGVKA 363

Query: 363 TMDAGIPLIVDAQIEISGQFSGSYTWGETHSTSEELGDTHEIEILPRTAVKVGLVATKGY 422
           T  AGIP+I   ++ IS +FSG YTWGET  T++E+  TH + + P T + V L+AT+GY
Sbjct: 364 TFRAGIPVIAQGRVTISAEFSGEYTWGETRETTDEIETTHTVVVPPMTKMTVSLLATQGY 423

Query: 423 CDVPFSYTRRDVLVSGEVRITEHDDGLFTGANSYNFHYDAGD 459
           CDVPFSY +RDVL++GE  I  ++DGL+TGAN YNF Y+A D
Sbjct: 424 CDVPFSYYQRDVLMNGETVIELYEDGLYTGANCYNFKYEAKD 464

BLAST of Spg030301 vs. NCBI nr
Match: XP_023544075.1 (uncharacterized protein LOC111803769 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 401.7 bits (1031), Expect = 8.3e-108
Identity = 198/459 (43.14%), Postives = 293/459 (63.83%), Query Frame = 0

Query: 1   MAVPRFSAFKSSHNGKYLNCVPADDNTIPNYVQYGSDEIATTYTKFSIEAASTGKDHVHI 60
           M +PR+ AFKS  +  YL  V  +D  I  Y++Y +  + T +TK+ +E A  GK +V+I
Sbjct: 1   MVLPRYVAFKSKADDLYLKYV-KEDVQIHGYIKYDAHNVMTPHTKYEVEPAKVGKGYVNI 60

Query: 61  RSIYNQKYLKVESTSEQWITATAVEANEEQSDWACTLFRIVYNSVTGAYQIIHVHYGKPV 120
           R  YN KY    S + +++ A A E NE+QS+WACTLF  +++    AY+I HV+ G   
Sbjct: 61  RCCYNNKYWVCPSINTRYVVAVADEPNEDQSNWACTLFEPIFDPDHQAYRIKHVYLGYNT 120

Query: 121 DLSNDLASRPNCLYI---YTDSSSHENHAVLDWETMVLFPQHVVFKGDNGKYLKIHNQDY 180
               D  +R  CLY+     D++  + H  +DWE+  + P++V FKGDNG YL+    + 
Sbjct: 121 CSWRDGLTRDKCLYMGYSTPDANLCDLHVAIDWESFYVLPKYVAFKGDNGSYLQACWVEG 180

Query: 181 HDFLRFYETDPHVSSVQQEVFVTSDGKLRIKGPN-NKFWRAAPNWIWVDSTDETSNNSYT 240
             +L+F   D    +V  EV  T+DG +RIK  +  KFWR +PNWIW DS D T+N+  T
Sbjct: 181 FPYLQFSSNDIGDPTVGNEVSTTNDGNVRIKSNHFEKFWRRSPNWIWADSHDTTANDKDT 240

Query: 241 LFQPYKINKNVVALRNLGMNYFCKRLTADWKEDCLDAIDSSMSKYTRMTVEEAILSRSVY 300
           LF P K++ N+VALRNLG N+FCKRLT + K  CL+A   +++K  R+ ++E +LSRS+Y
Sbjct: 241 LFWPVKVDNNIVALRNLGNNHFCKRLTTEGKTSCLNAGVPTITKEARLEIQELVLSRSIY 300

Query: 301 NVQYRLSEGRIYGESIIAMDFQGAVNNSNETNTLSLEFFYTKEESQNWSAILAAELSYTM 360
           NV + L + RIYG+ +I M    A N+SN+ NT+ ++  YT  ES  WS+ ++A+L    
Sbjct: 301 NVIFHLLDARIYGQHVITMANGDASNSSNKENTIDIKLRYTISESNTWSSTVSAKLGVKT 360

Query: 361 TMDAGIPLIVDAQIEISGQFSGSYTWGETHSTSEELGDTHEIEILPRTAVKVGLVATKGY 420
           T+  GIPLI + +IE+S +FSG YTWGET S S E+   +++ + P + V+V L+ATKG+
Sbjct: 361 TIQTGIPLIAEGKIEMSAEFSGGYTWGETQSKSSEIETVYKVTVPPMSRVRVSLLATKGH 420

Query: 421 CDVPFSYTRRDVLVSGEVRITEHDDGLFTGANSYNFHYD 456
           CDVP+SYT+RD L++G+     +DDG++T  N YNF Y+
Sbjct: 421 CDVPYSYTQRDTLMNGDQVTNYYDDGIYTAVNCYNFKYE 458

BLAST of Spg030301 vs. NCBI nr
Match: KAG6603266.1 (hypothetical protein SDJN03_03875, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 400.6 bits (1028), Expect = 1.9e-107
Identity = 198/459 (43.14%), Postives = 291/459 (63.40%), Query Frame = 0

Query: 1   MAVPRFSAFKSSHNGKYLNCVPADDNTIPNYVQYGSDEIATTYTKFSIEAASTGKDHVHI 60
           M  PR+ AFKS  +  YL  V  +D  I  Y++Y +  + T +TK+ +E A  GK +V+I
Sbjct: 1   MVFPRYVAFKSKADDLYLKYV-KEDVQIHGYIKYDAHNVMTPHTKYEVEPAKVGKGYVNI 60

Query: 61  RSIYNQKYLKVESTSEQWITATAVEANEEQSDWACTLFRIVYNSVTGAYQIIHVHYGKPV 120
           R  YN KY    S + +++ A A E NE+QS+WACTLF  +++    AY+I HV+ G   
Sbjct: 61  RCCYNNKYWVCPSINTRYVVAVADEPNEDQSNWACTLFEPIFDPDHQAYRIKHVYLGYNT 120

Query: 121 DLSNDLASRPNCLYI---YTDSSSHENHAVLDWETMVLFPQHVVFKGDNGKYLKIHNQDY 180
               D  +R  CLY+     D++  + H  +DWE+  + P++V FKGDNG YL+    + 
Sbjct: 121 CSWRDGLTRDKCLYMGYSTPDANLCDLHIAIDWESFYVLPKYVAFKGDNGSYLQACWIEG 180

Query: 181 HDFLRFYETDPHVSSVQQEVFVTSDGKLRIKGPN-NKFWRAAPNWIWVDSTDETSNNSYT 240
             +L+F   D    +V  EV  T+DG +RIK  +  KFWR +PNWIW DS D T+NN  T
Sbjct: 181 FPYLQFSSNDIGDPTVGNEVSTTNDGNVRIKSNHFEKFWRRSPNWIWADSNDTTANNKDT 240

Query: 241 LFQPYKINKNVVALRNLGMNYFCKRLTADWKEDCLDAIDSSMSKYTRMTVEEAILSRSVY 300
           LF P K++ N+VALRN G N+FCKRLT + K  CL+A   +++K  R+ ++E +LSRS+Y
Sbjct: 241 LFWPVKVDNNIVALRNSGNNHFCKRLTTEGKTSCLNAGVPTITKEARLEIQELVLSRSIY 300

Query: 301 NVQYRLSEGRIYGESIIAMDFQGAVNNSNETNTLSLEFFYTKEESQNWSAILAAELSYTM 360
           NV + L + RIYG+ +I M    A N+SN+ NT+ ++  YT  ES  WS+ ++A+L    
Sbjct: 301 NVIFHLLDARIYGQHVITMANGDASNSSNKENTIDIKLRYTISESNTWSSTVSAKLGVKT 360

Query: 361 TMDAGIPLIVDAQIEISGQFSGSYTWGETHSTSEELGDTHEIEILPRTAVKVGLVATKGY 420
           T+  GIPLI + +IE+S +FSG YTWGET S S E+   +++ + P + V+V L+ATKG+
Sbjct: 361 TIQTGIPLIAEGKIEMSAEFSGGYTWGETQSKSSEIETVYKVTVPPMSRVRVSLLATKGH 420

Query: 421 CDVPFSYTRRDVLVSGEVRITEHDDGLFTGANSYNFHYD 456
           CDVP+SYT+RD L++G+     +DDG++T  N YNF Y+
Sbjct: 421 CDVPYSYTQRDTLMNGDQVTNYYDDGIYTAVNCYNFKYE 458

BLAST of Spg030301 vs. NCBI nr
Match: XP_022967680.1 (uncharacterized protein LOC111467133 [Cucurbita maxima])

HSP 1 Score: 400.2 bits (1027), Expect = 2.4e-107
Identity = 197/459 (42.92%), Postives = 292/459 (63.62%), Query Frame = 0

Query: 1   MAVPRFSAFKSSHNGKYLNCVPADDNTIPNYVQYGSDEIATTYTKFSIEAASTGKDHVHI 60
           M +PR+ AFKS  +  YL  V  +D  I  Y++Y +  + T +TK+ +E A  GK +V+I
Sbjct: 1   MVLPRYVAFKSKADDLYLKYV-KEDVQIHGYIKYDAHNVVTPHTKYEVEPAKVGKGYVNI 60

Query: 61  RSIYNQKYLKVESTSEQWITATAVEANEEQSDWACTLFRIVYNSVTGAYQIIHVHYGKPV 120
           R  YN KY    S + +++ A A E NE+QS+WACTLF  +++    AY+I HV++G   
Sbjct: 61  RCCYNNKYWVCPSINTRYVVAVADEPNEDQSNWACTLFEPIFDPDHQAYRIKHVYFGYNT 120

Query: 121 DLSNDLASRPNCLYI---YTDSSSHENHAVLDWETMVLFPQHVVFKGDNGKYLKIHNQDY 180
               D  +R  CLY+     D++  + H  +DWE+  + P+HV FKGDNG YL+    + 
Sbjct: 121 CSWRDGLARDRCLYMGYSTPDANLCDLHIAIDWESFYVLPKHVAFKGDNGSYLQACWIEG 180

Query: 181 HDFLRFYETDPHVSSVQQEVFVTSDGKLRIKGPN-NKFWRAAPNWIWVDSTDETSNNSYT 240
             +L+F   D    +V  EV  T+DG +RIK  +  KFWR + NWIW DS D T++N  T
Sbjct: 181 FPYLQFSSHDIGDPTVGNEVSTTNDGNVRIKSNHFEKFWRRSSNWIWADSNDTTASNKDT 240

Query: 241 LFQPYKINKNVVALRNLGMNYFCKRLTADWKEDCLDAIDSSMSKYTRMTVEEAILSRSVY 300
           LF P ++  N+VALRNLG N+FCKRLT + K  CL+A  S+++K  R+ ++E +LSRS+Y
Sbjct: 241 LFWPVRVENNIVALRNLGNNHFCKRLTTEGKTSCLNAGVSTITKEARLEIQELVLSRSIY 300

Query: 301 NVQYRLSEGRIYGESIIAMDFQGAVNNSNETNTLSLEFFYTKEESQNWSAILAAELSYTM 360
           NV + L + RIYG+ +I M    A N+SN+ NT+ ++  YT  E+  WS+ ++A+L    
Sbjct: 301 NVIFHLLDARIYGQHVITMANGDASNSSNKENTIDIKLRYTISENNTWSSTVSAKLGVKT 360

Query: 361 TMDAGIPLIVDAQIEISGQFSGSYTWGETHSTSEELGDTHEIEILPRTAVKVGLVATKGY 420
           T+  GIPLI   +IE+S +FSG YTWGET S S E+   +++ + P + V+V L+ATKG+
Sbjct: 361 TIQTGIPLIAKGKIEMSAEFSGGYTWGETRSKSSEIETVYKVTVPPMSRVRVSLLATKGH 420

Query: 421 CDVPFSYTRRDVLVSGEVRITEHDDGLFTGANSYNFHYD 456
           CDVP+SYT+RD L++G+     +DDG++T  N YNF Y+
Sbjct: 421 CDVPYSYTQRDTLMNGDQVTNYYDDGIYTAVNCYNFKYE 458

BLAST of Spg030301 vs. ExPASy Swiss-Prot
Match: Q66S21 (Natterin-2 OS=Thalassophryne nattereri OX=289382 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 2.3e-04
Identity = 38/158 (24.05%), Postives = 77/158 (48.73%), Query Frame = 0

Query: 272 DAIDSSMSKYTRMTVEEAILSRSVYNVQYRLSEGRIYGESIIAMDFQGAVNNSN---ETN 331
           D  ++    Y  +T+ + ++ +++ +V+Y+ +EG    +    +  +  VNN +    T 
Sbjct: 156 DGAETWTKTYQALTMNKDVIEQAMKDVKYQ-TEGVTVIKGKPEVMRRSTVNNQHCKEVTK 215

Query: 332 TLSLEFFYTKEES--QNWSAILAAELSYTMTMDAGIPLIVDAQIEISGQFSGSYTWGETH 391
           T++L    TK+ S  + W    +     T T+ AGIP +  A +EIS Q +  +  G + 
Sbjct: 216 TVTL----TKDISTDERWDVTNSVTFGVTTTVTAGIPDVSSASLEISMQATMDFAHGASK 275

Query: 392 STSEELGDTHEIEILPRTAVKVGLVATKGYCDVPFSYT 425
           + ++    T  + + P+ +  V +VA     D+PF+ T
Sbjct: 276 TETQSYMVTVSVPVPPKQSCTVSMVAQVNKADIPFTAT 308

BLAST of Spg030301 vs. ExPASy TrEMBL
Match: A0A5D3DML5 (Agglutinin domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G003250 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 3.2e-174
Identity = 291/463 (62.85%), Postives = 358/463 (77.32%), Query Frame = 0

Query: 1   MAVPRFSAFKSSHNGKYLNCVPADDNTIPNYVQYGSDEIATTYTKFSIEAA-STGKDHVH 60
           M VPRF+ F+SS N KY+N V +DD  IPN+VQY SD +AT+ TKFS  A+  T K+  H
Sbjct: 1   MVVPRFAGFRSSKNQKYMNVVKSDDEKIPNFVQYSSDNLATSTTKFSFVASHDTAKNLYH 60

Query: 61  IRSIYNQKYLKVESTSEQWITATAVEANEEQSDWACTLFRIVYNSVTGAYQIIHVHYGKP 120
           I  IYNQKYL+ ES+S +WI ATA+E NE+QSDW CTLFRI + S    Y + H++YG+P
Sbjct: 61  IVCIYNQKYLRAESSSSRWICATALEPNEDQSDWGCTLFRITWGSTEQRYDLFHMYYGQP 120

Query: 121 VDLSNDLASRPNCLYIYTDSSSHENHAVLDWETMVLFPQHVVFKGDNGKYLKIHNQDYHD 180
           VDL ND +S PNCLYIY  SS+  NH+VLDWE M + PQHVVFKGDN KYLKIHNQD HD
Sbjct: 121 VDLKND-SSHPNCLYIYDQSSTIYNHSVLDWEVMSILPQHVVFKGDNNKYLKIHNQDSHD 180

Query: 181 FLRFYETDPHVSSVQQEVFVTSDGKLRIKGPNNKFWRAAPNWIWVDSTDETSNNSYTLFQ 240
           FLRF   DP  SSVQQEV +TSDGK+RIKGPNNKFWRA+PNWIW+DSTD +SNN  T+FQ
Sbjct: 181 FLRFLLDDPQDSSVQQEVVITSDGKIRIKGPNNKFWRASPNWIWIDSTDTSSNNQATVFQ 240

Query: 241 PYKINKNVVALRNLGMNYFCKRLTADWKEDCLDAIDSSMSKYTRMTVEEAILSRSVYNVQ 300
           P KI+ NV+ LRNLG N FC R +ADWKE CL A D +MS Y+R+TVEEA+LSR +YNVQ
Sbjct: 241 PCKISNNVICLRNLGNNMFCSRTSADWKESCLSANDHAMSNYSRLTVEEAVLSREIYNVQ 300

Query: 301 YRLSEGRIYGESIIAMDFQGAVNNSNETNTLSLEFFYTKEESQNWSAILAAELSYTMTMD 360
           YRLS+GRIYGE+I+A+DFQGA+N S+E N +   F Y +EESQ WS+ +  +L+Y+MT++
Sbjct: 301 YRLSDGRIYGENILAVDFQGAMNGSDEVNHMDFNFSYEEEESQCWSSTVGMKLAYSMTIE 360

Query: 361 AGIPLIVDAQIEISGQFSGSYTWGETHSTSEELGDTHEIEILPRTAVKVGLVATKGYCDV 420
           AGIPLI+D  I +SG+ S SYTWG+T+  S     ++ +E+ PRT+VKV  + TKGYCDV
Sbjct: 361 AGIPLIMDTNIVMSGEISESYTWGQTNKKSTPKRTSYSLEVQPRTSVKVSAMLTKGYCDV 420

Query: 421 PFSYTRRDVLVSGEVRITEHDDGLFTGANSYNFHYDAGDVKRL 463
           PFSYT+RD+LV G+ R TEHDDGLFTG N YNFHY + DV  L
Sbjct: 421 PFSYTQRDILVDGQTRTTEHDDGLFTGFNCYNFHYQSEDVTSL 462

BLAST of Spg030301 vs. ExPASy TrEMBL
Match: A0A1S3C8I1 (uncharacterized protein LOC103497997 OS=Cucumis melo OX=3656 GN=LOC103497997 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 3.2e-174
Identity = 291/463 (62.85%), Postives = 358/463 (77.32%), Query Frame = 0

Query: 1   MAVPRFSAFKSSHNGKYLNCVPADDNTIPNYVQYGSDEIATTYTKFSIEAA-STGKDHVH 60
           M VPRF+ F+SS N KY+N V +DD  IPN+VQY SD +AT+ TKFS  A+  T K+  H
Sbjct: 1   MVVPRFAGFRSSKNQKYMNVVKSDDEKIPNFVQYSSDNLATSTTKFSFVASHDTAKNLYH 60

Query: 61  IRSIYNQKYLKVESTSEQWITATAVEANEEQSDWACTLFRIVYNSVTGAYQIIHVHYGKP 120
           I  IYNQKYL+ ES+S +WI ATA+E NE+QSDW CTLFRI + S    Y + H++YG+P
Sbjct: 61  IVCIYNQKYLRAESSSSRWICATALEPNEDQSDWGCTLFRITWGSTEQRYDLFHMYYGQP 120

Query: 121 VDLSNDLASRPNCLYIYTDSSSHENHAVLDWETMVLFPQHVVFKGDNGKYLKIHNQDYHD 180
           VDL ND +S PNCLYIY  SS+  NH+VLDWE M + PQHVVFKGDN KYLKIHNQD HD
Sbjct: 121 VDLKND-SSHPNCLYIYDQSSTIYNHSVLDWEVMSILPQHVVFKGDNNKYLKIHNQDSHD 180

Query: 181 FLRFYETDPHVSSVQQEVFVTSDGKLRIKGPNNKFWRAAPNWIWVDSTDETSNNSYTLFQ 240
           FLRF   DP  SSVQQEV +TSDGK+RIKGPNNKFWRA+PNWIW+DSTD +SNN  T+FQ
Sbjct: 181 FLRFLLDDPQDSSVQQEVVITSDGKIRIKGPNNKFWRASPNWIWIDSTDTSSNNQATVFQ 240

Query: 241 PYKINKNVVALRNLGMNYFCKRLTADWKEDCLDAIDSSMSKYTRMTVEEAILSRSVYNVQ 300
           P KI+ NV+ LRNLG N FC R +ADWKE CL A D +MS Y+R+TVEEA+LSR +YNVQ
Sbjct: 241 PCKISNNVICLRNLGNNMFCSRTSADWKESCLSANDHAMSNYSRLTVEEAVLSREIYNVQ 300

Query: 301 YRLSEGRIYGESIIAMDFQGAVNNSNETNTLSLEFFYTKEESQNWSAILAAELSYTMTMD 360
           YRLS+GRIYGE+I+A+DFQGA+N S+E N +   F Y +EESQ WS+ +  +L+Y+MT++
Sbjct: 301 YRLSDGRIYGENILAVDFQGAMNGSDEVNHMDFNFSYEEEESQCWSSTVGMKLAYSMTIE 360

Query: 361 AGIPLIVDAQIEISGQFSGSYTWGETHSTSEELGDTHEIEILPRTAVKVGLVATKGYCDV 420
           AGIPLI+D  I +SG+ S SYTWG+T+  S     ++ +E+ PRT+VKV  + TKGYCDV
Sbjct: 361 AGIPLIMDTNIVMSGEISESYTWGQTNKKSTPKRTSYSLEVQPRTSVKVSAMLTKGYCDV 420

Query: 421 PFSYTRRDVLVSGEVRITEHDDGLFTGANSYNFHYDAGDVKRL 463
           PFSYT+RD+LV G+ R TEHDDGLFTG N YNFHY + DV  L
Sbjct: 421 PFSYTQRDILVDGQTRTTEHDDGLFTGFNCYNFHYQSEDVTSL 462

BLAST of Spg030301 vs. ExPASy TrEMBL
Match: A0A6J1DS59 (uncharacterized protein LOC111023023 OS=Momordica charantia OX=3673 GN=LOC111023023 PE=4 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 1.4e-113
Identity = 212/462 (45.89%), Postives = 296/462 (64.07%), Query Frame = 0

Query: 3   VPRFSAFKSSHNGKYLNCVPADDNTIPNYVQYGSDEIATTYTKFSIEAASTGKDHVHIRS 62
           +P++   +S  +  YL  V  +D  I  YV+Y S ++ T YTK+ +E A  GK +V+IR 
Sbjct: 4   LPKYVGLRSLADDLYLRFV-KEDVQIHGYVKYDSHDVVTPYTKYEVEPAKVGKRYVNIRC 63

Query: 63  IYNQKYLKVESTSEQWITATAVEANEEQSDWACTLFRIVYNSVTGAYQIIHVHYGKPVDL 122
            Y+ KY    S + +++ A A E NE++S+W+CTLF  +++S   AY+I HV  G     
Sbjct: 64  CYSNKYWVRPSMNSRYVVAAADEPNEDESNWSCTLFEPIHDSGNQAYRIKHVQLGFNTCS 123

Query: 123 SNDLASRPNCLYIYTDSSSHEN----HAVLDWETMVLFPQHVVFKGDN-GKYLKIHNQDY 182
           S D +    CL+I T S+   N    H V+DWE+  +FP+HV FKG N GK+LK      
Sbjct: 124 SRDGSIHNECLFIMTSSAPPRNFGDLHIVMDWESFFIFPKHVAFKGGNRGKFLKAIWLQR 183

Query: 183 HDFLRFYETDPHVSSVQQEVFVTSDGKLRIKGPN-NKFWRAAPNWIWVDSTDETSNNSYT 242
           H++  F   D    +V  EV  T  G +RIK  +  KFWR +PNWIW DSTD T+NN +T
Sbjct: 184 HEYQEFSANDMADPAVGNEVSFTRHGDVRIKSNHFGKFWRLSPNWIWADSTDPTANNKHT 243

Query: 243 LFQPYKINKNVVALRNLGMNYFCKRLTADWKEDCLDAIDSSMSKYTRMTVEEAILSRSVY 302
           LF+P K+  N+VALRNLG N+FC  LT + K DCL+A  S+++   RM + E +LSRS+Y
Sbjct: 244 LFKPVKVANNIVALRNLGNNHFCIGLTTEGKTDCLNAWVSTITNEARMEIHELVLSRSIY 303

Query: 303 NVQYRLSEGRIYGESIIAMDFQGAVNNSNETNTLSLEFFYTKEESQNWSAILAAELSYTM 362
           NV + L + RIYGE+IIAM    AVNN+NE NT+++ F YT   +  WS+ L+A+L    
Sbjct: 304 NVDFHLFDARIYGENIIAMANGNAVNNTNEPNTITIRFSYTNRVTNTWSSTLSAKLGVKA 363

Query: 363 TMDAGIPLIVDAQIEISGQFSGSYTWGETHSTSEELGDTHEIEILPRTAVKVGLVATKGY 422
           T  AGIP+I   ++ IS +FSG YTWGET  T++E+  TH + + P T + V L+AT+GY
Sbjct: 364 TFRAGIPVIAQGRVTISAEFSGEYTWGETRETTDEIETTHTVVVPPMTKMTVSLLATQGY 423

Query: 423 CDVPFSYTRRDVLVSGEVRITEHDDGLFTGANSYNFHYDAGD 459
           CDVPFSY +RDVL++GE  I  ++DGL+TGAN YNF Y+A D
Sbjct: 424 CDVPFSYYQRDVLMNGETVIELYEDGLYTGANCYNFKYEAKD 464

BLAST of Spg030301 vs. ExPASy TrEMBL
Match: A0A6J1HV52 (uncharacterized protein LOC111467133 OS=Cucurbita maxima OX=3661 GN=LOC111467133 PE=3 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 1.2e-107
Identity = 197/459 (42.92%), Postives = 292/459 (63.62%), Query Frame = 0

Query: 1   MAVPRFSAFKSSHNGKYLNCVPADDNTIPNYVQYGSDEIATTYTKFSIEAASTGKDHVHI 60
           M +PR+ AFKS  +  YL  V  +D  I  Y++Y +  + T +TK+ +E A  GK +V+I
Sbjct: 1   MVLPRYVAFKSKADDLYLKYV-KEDVQIHGYIKYDAHNVVTPHTKYEVEPAKVGKGYVNI 60

Query: 61  RSIYNQKYLKVESTSEQWITATAVEANEEQSDWACTLFRIVYNSVTGAYQIIHVHYGKPV 120
           R  YN KY    S + +++ A A E NE+QS+WACTLF  +++    AY+I HV++G   
Sbjct: 61  RCCYNNKYWVCPSINTRYVVAVADEPNEDQSNWACTLFEPIFDPDHQAYRIKHVYFGYNT 120

Query: 121 DLSNDLASRPNCLYI---YTDSSSHENHAVLDWETMVLFPQHVVFKGDNGKYLKIHNQDY 180
               D  +R  CLY+     D++  + H  +DWE+  + P+HV FKGDNG YL+    + 
Sbjct: 121 CSWRDGLARDRCLYMGYSTPDANLCDLHIAIDWESFYVLPKHVAFKGDNGSYLQACWIEG 180

Query: 181 HDFLRFYETDPHVSSVQQEVFVTSDGKLRIKGPN-NKFWRAAPNWIWVDSTDETSNNSYT 240
             +L+F   D    +V  EV  T+DG +RIK  +  KFWR + NWIW DS D T++N  T
Sbjct: 181 FPYLQFSSHDIGDPTVGNEVSTTNDGNVRIKSNHFEKFWRRSSNWIWADSNDTTASNKDT 240

Query: 241 LFQPYKINKNVVALRNLGMNYFCKRLTADWKEDCLDAIDSSMSKYTRMTVEEAILSRSVY 300
           LF P ++  N+VALRNLG N+FCKRLT + K  CL+A  S+++K  R+ ++E +LSRS+Y
Sbjct: 241 LFWPVRVENNIVALRNLGNNHFCKRLTTEGKTSCLNAGVSTITKEARLEIQELVLSRSIY 300

Query: 301 NVQYRLSEGRIYGESIIAMDFQGAVNNSNETNTLSLEFFYTKEESQNWSAILAAELSYTM 360
           NV + L + RIYG+ +I M    A N+SN+ NT+ ++  YT  E+  WS+ ++A+L    
Sbjct: 301 NVIFHLLDARIYGQHVITMANGDASNSSNKENTIDIKLRYTISENNTWSSTVSAKLGVKT 360

Query: 361 TMDAGIPLIVDAQIEISGQFSGSYTWGETHSTSEELGDTHEIEILPRTAVKVGLVATKGY 420
           T+  GIPLI   +IE+S +FSG YTWGET S S E+   +++ + P + V+V L+ATKG+
Sbjct: 361 TIQTGIPLIAKGKIEMSAEFSGGYTWGETRSKSSEIETVYKVTVPPMSRVRVSLLATKGH 420

Query: 421 CDVPFSYTRRDVLVSGEVRITEHDDGLFTGANSYNFHYD 456
           CDVP+SYT+RD L++G+     +DDG++T  N YNF Y+
Sbjct: 421 CDVPYSYTQRDTLMNGDQVTNYYDDGIYTAVNCYNFKYE 458

BLAST of Spg030301 vs. ExPASy TrEMBL
Match: A0A6J1DPH8 (uncharacterized protein LOC111023006 OS=Momordica charantia OX=3673 GN=LOC111023006 PE=4 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 9.9e-107
Identity = 198/459 (43.14%), Postives = 284/459 (61.87%), Query Frame = 0

Query: 1   MAVPRFSAFKSSHNGKYLNCVPADDNTIPNYVQYGSDEIATTYTKFSIEAASTGKDHVHI 60
           M +PR+ AFKS  +  YL  +  +D  I  YV+Y +  + T+YTK+ +E A  GK +V+I
Sbjct: 1   MVLPRYVAFKSKADDLYLKFI-KEDVQIHGYVKYDAHSVVTSYTKYEVEPAKVGKGYVNI 60

Query: 61  RSIYNQKYLKVESTSEQWITATAVEANEEQSDWACTLFRIVYNSVTGAYQIIHVHYGKPV 120
           R  YN KY    S   Q++ A A E NE+QS+WACTLF   Y+    AY+I HV+ G   
Sbjct: 61  RCCYNNKYWVCPSMYTQYVVAAADERNEDQSNWACTLFEPTYDPYHKAYRIKHVYLGYNT 120

Query: 121 DLSNDLASRPNCL---YIYTDSSSHENHAVLDWETMVLFPQHVVFKGDNGKYLKIHNQDY 180
               D  +R  CL   +   D++  + H  +DWE+  + P+HV FKGDNG YL     + 
Sbjct: 121 CSWRDGTTRDRCLNMGFSNPDANLCDLHIAVDWESFFVLPKHVAFKGDNGSYLSAQWIED 180

Query: 181 HDFLRFYETDPHVSSVQQEVFVTSDGKLRIKGPN-NKFWRAAPNWIWVDSTDETSNNSYT 240
             +L+F   D     V  EV +TSDG +RIK     KFWR +PNWIW D+TD TSN+   
Sbjct: 181 LPYLQFSLDDIGDPKVGNEVLITSDGNVRIKSNYFGKFWRRSPNWIWADTTDTTSNDQDI 240

Query: 241 LFQPYKINKNVVALRNLGMNYFCKRLTADWKEDCLDAIDSSMSKYTRMTVEEAILSRSVY 300
           LF P ++  NVVALRNLG N FCKRLT D K +CL+A   ++++  R+ +EE +LSRS+Y
Sbjct: 241 LFWPVRVENNVVALRNLGNNSFCKRLTTDGKTNCLNAAVPTITREARLVIEELVLSRSIY 300

Query: 301 NVQYRLSEGRIYGESIIAMDFQGAVNNSNETNTLSLEFFYTKEESQNWSAILAAELSYTM 360
           N+ + L + RIY  S+I M    A N+S+E NT+ ++  YT   S  WS+ L+A+L    
Sbjct: 301 NIIFHLLDARIYDASVITMANGDASNDSSEANTIDIKLSYTNSVSNTWSSTLSAKLGVKT 360

Query: 361 TMDAGIPLIVDAQIEISGQFSGSYTWGETHSTSEELGDTHEIEILPRTAVKVGLVATKGY 420
           +M  GIPLI + +I+I  +FSGSY+WGET   S  +  T+++ + P T V+V L+AT+ +
Sbjct: 361 SMQTGIPLIAEGKIQICAEFSGSYSWGETRLKSSVIETTYKVTVPPMTRVRVSLLATQAH 420

Query: 421 CDVPFSYTRRDVLVSGEVRITEHDDGLFTGANSYNFHYD 456
           CD+P+SYT+RD L++G+     +DDG++T  N YNF Y+
Sbjct: 421 CDIPYSYTQRDTLINGDQVTNYYDDGIYTSVNCYNFKYE 458