Spg030254 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg030254
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Calcium-binding EF-hand family protein, putative
Location	scaffold6: 15538192 .. 15538470 (+)
RNA-Seq Expression	Spg030254
Synteny	Spg030254

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCATCGCGAAGTCGAAACGTACGAGCAGAGGCCGAAGCTATCAAGAGAGCAACTATGCCTAATCTTCAGAAACCATGATGGCGACCGTGACGGCCGCCTTAGCTTCGCCGAGCTCACTGAAGCTTTTCGGTACATCGGAGCCCTTCTACCGCCGTTTAGAGCTTTCTGCGCTCTTCTTCATGTTGATTCTGATAGAGATGGATTCATCTTCATCGACCAGAAGGATTTCGAATTGCTTGTCAATTATGCCGAGGAACGGAAATACACCGTCATTTGA

mRNA sequence

ATGCATCGCGAAGTCGAAACGTACGAGCAGAGGCCGAAGCTATCAAGAGAGCAACTATGCCTAATCTTCAGAAACCATGATGGCGACCGTGACGGCCGCCTTAGCTTCGCCGAGCTCACTGAAGCTTTTCGGTACATCGGAGCCCTTCTACCGCCGTTTAGAGCTTTCTGCGCTCTTCTTCATGTTGATTCTGATAGAGATGGATTCATCTTCATCGACCAGAAGGATTTCGAATTGCTTGTCAATTATGCCGAGGAACGGAAATACACCGTCATTTGA

Coding sequence (CDS)

ATGCATCGCGAAGTCGAAACGTACGAGCAGAGGCCGAAGCTATCAAGAGAGCAACTATGCCTAATCTTCAGAAACCATGATGGCGACCGTGACGGCCGCCTTAGCTTCGCCGAGCTCACTGAAGCTTTTCGGTACATCGGAGCCCTTCTACCGCCGTTTAGAGCTTTCTGCGCTCTTCTTCATGTTGATTCTGATAGAGATGGATTCATCTTCATCGACCAGAAGGATTTCGAATTGCTTGTCAATTATGCCGAGGAACGGAAATACACCGTCATTTGA

Protein sequence

MHREVETYEQRPKLSREQLCLIFRNHDGDRDGRLSFAELTEAFRYIGALLPPFRAFCALLHVDSDRDGFIFIDQKDFELLVNYAEERKYTVI

Homology

[Close]

BLAST of Spg030254 vs. NCBI nr
Match: KAG7024248.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 134.4 bits (337), Expect = 4.9e-28
Identity = 68/92 (73.91%), Postives = 75/92 (81.52%), Query Frame = 0


Query: 1  MHREVETYEQRPKLSREQLCLIFRNHDGDRDGRLSFAELTEAFRYIGALLPPFRAFCALL 60
          M R+ +TY+ RPKLSREQL +IFRNHDGD DGRLS  ELT AFRYIGA LP +RA  ALL
Sbjct: 1  MARKSKTYQPRPKLSREQLSVIFRNHDGDGDGRLSTTELTAAFRYIGAFLPSYRALHALL 60

Query: 61 HVDSDRDGFIFIDQKDFELLVNYAEERKYTVI 93
          H DSD DG  FI +KDFELLVNYAE+RKYTVI
Sbjct: 61 HTDSDGDG--FIGEKDFELLVNYAEKRKYTVI 90

[Close]

BLAST of Spg030254 vs. NCBI nr
Match: KAA0064376.1 (putative calcium-binding protein CML27 [Cucumis melo var. makuwa] >TYK20211.1 putative calcium-binding protein CML27 [Cucumis melo var. makuwa])

HSP 1 Score: 134.4 bits (337), Expect = 4.9e-28
Identity = 67/92 (72.83%), Postives = 74/92 (80.43%), Query Frame = 0


Query: 1  MHREVETYEQRPKLSREQLCLIFRNHDGDRDGRLSFAELTEAFRYIGALLPPFRAFCALL 60
          M R  +TY  R +LSREQL LIFRNHD D+DGRLS  ELTEAFRYIGA LPP+RA  AL 
Sbjct: 1  MSRRSKTYHPRARLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPPYRARHALF 60

Query: 61 HVDSDRDGFIFIDQKDFELLVNYAEERKYTVI 93
          H DSDRDG  FI +KDFELLVNYAE+R+YTVI
Sbjct: 61 HADSDRDG--FISEKDFELLVNYAEQRQYTVI 90

[Close]

BLAST of Spg030254 vs. NCBI nr
Match: KAG6591370.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 131.3 bits (329), Expect = 4.2e-27
Identity = 66/90 (73.33%), Postives = 73/90 (81.11%), Query Frame = 0


Query: 1  MHREVETYEQRPKLSREQLCLIFRNHDGDRDGRLSFAELTEAFRYIGALLPPFRAFCALL 60
          M R+ +TY+ RPKLSREQL +IFRNHDGD DGRLS  ELT AFRYIGA LP +RA  ALL
Sbjct: 1  MARKSKTYQPRPKLSREQLSVIFRNHDGDGDGRLSTTELTAAFRYIGAFLPSYRALHALL 60

Query: 61 HVDSDRDGFIFIDQKDFELLVNYAEERKYT 91
          H DSD DG  FI +KDFELLVNYAE+RKYT
Sbjct: 61 HTDSDGDG--FIGEKDFELLVNYAEKRKYT 88

[Close]

BLAST of Spg030254 vs. NCBI nr
Match: KGN55185.1 (hypothetical protein Csa_012915 [Cucumis sativus])

HSP 1 Score: 128.6 bits (322), Expect = 2.7e-26
Identity = 65/92 (70.65%), Postives = 73/92 (79.35%), Query Frame = 0


Query: 1  MHREVETYEQRPKLSREQLCLIFRNHDGDRDGRLSFAELTEAFRYIGALLPPFRAFCALL 60
          M R+ + Y  R KLSREQL LIFRNHD D+DGRLS  ELTEAFRYIGA LP +RA  ALL
Sbjct: 1  MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 61 HVDSDRDGFIFIDQKDFELLVNYAEERKYTVI 93
          H DSDRDG  FI +KDFE LVNYAE+R+YT+I
Sbjct: 61 HADSDRDG--FIGEKDFESLVNYAEQRQYTII 90

[Close]

BLAST of Spg030254 vs. NCBI nr
Match: KAG6600743.1 (Calcium-binding protein CML38, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031384.1 Calcium-binding protein CML38, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 127.9 bits (320), Expect = 4.6e-26
Identity = 64/92 (69.57%), Postives = 76/92 (82.61%), Query Frame = 0


Query: 1  MHREVETYEQRPKLSREQLCLIFRNHDGDRDGRLSFAELTEAFRYIGALLPPFRAFCALL 60
          M R+ + Y QRPK+SR+QL +IFRNHD D DGRLS AELT+AF YIGALLP FRA  ALL
Sbjct: 1  MPRKTKMYHQRPKISRQQLRVIFRNHDRDGDGRLSSAELTKAFHYIGALLPSFRACHALL 60

Query: 61 HVDSDRDGFIFIDQKDFELLVNYAEERKYTVI 93
          + DSDRDG  FID+KDFE L++YAE+R+YTVI
Sbjct: 61 YTDSDRDG--FIDEKDFEQLIDYAEKRQYTVI 90

[Close]

BLAST of Spg030254 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 5.1e-04
Identity = 20/53 (37.74%), Postives = 31/53 (58.49%), Query Frame = 0


Query: 22 IFRNHDGDRDGRLSFAELTEAFRYIGALLPPFRAFCALLHVDSDRDGFIFIDQ 75
          +FR +D + DG++S  EL    R +GA L P      +  +DSDRDGF+ + +
Sbjct: 9  VFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSE 61

[Close]

BLAST of Spg030254 vs. ExPASy TrEMBL
Match: A0A5A7VDB9 (Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003480 PE=4 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.4e-28
Identity = 67/92 (72.83%), Postives = 74/92 (80.43%), Query Frame = 0


Query: 1  MHREVETYEQRPKLSREQLCLIFRNHDGDRDGRLSFAELTEAFRYIGALLPPFRAFCALL 60
          M R  +TY  R +LSREQL LIFRNHD D+DGRLS  ELTEAFRYIGA LPP+RA  AL 
Sbjct: 1  MSRRSKTYHPRARLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPPYRARHALF 60

Query: 61 HVDSDRDGFIFIDQKDFELLVNYAEERKYTVI 93
          H DSDRDG  FI +KDFELLVNYAE+R+YTVI
Sbjct: 61 HADSDRDG--FISEKDFELLVNYAEQRQYTVI 90

[Close]

BLAST of Spg030254 vs. ExPASy TrEMBL
Match: A0A0A0KZR6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639730 PE=4 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.3e-26
Identity = 65/92 (70.65%), Postives = 73/92 (79.35%), Query Frame = 0


Query: 1  MHREVETYEQRPKLSREQLCLIFRNHDGDRDGRLSFAELTEAFRYIGALLPPFRAFCALL 60
          M R+ + Y  R KLSREQL LIFRNHD D+DGRLS  ELTEAFRYIGA LP +RA  ALL
Sbjct: 1  MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 61 HVDSDRDGFIFIDQKDFELLVNYAEERKYTVI 93
          H DSDRDG  FI +KDFE LVNYAE+R+YT+I
Sbjct: 61 HADSDRDG--FIGEKDFESLVNYAEQRQYTII 90

[Close]

BLAST of Spg030254 vs. ExPASy TrEMBL
Match: A0A6J1CS20 (calmodulin-like protein 5 OS=Momordica charantia OX=3673 GN=LOC111014054 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 6.5e-10
Identity = 38/80 (47.50%), Postives = 52/80 (65.00%), Query Frame = 0


Query: 13 KLSREQLCLIFRNHDGDRDGRLSFAELTEAFRYIGALLPPFRAFCALLHVDSDRDGFIFI 72
          KL RE++  IFR HD + DG LS  EL+ AF  +GALLP +RA C +L  D+D DG   I
Sbjct: 13 KLLREEVKAIFRAHDINGDGHLSIKELSRAFGSLGALLPLYRAVCGILAADADGDG--LI 72

Query: 73 DQKDFELLVNYAEERKYTVI 93
            ++ E +V+YA + KY +I
Sbjct: 73 TDEELEKVVDYAVKCKYDII 90

[Close]

BLAST of Spg030254 vs. ExPASy TrEMBL
Match: A0A5N6RQU3 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_018534 PE=4 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 8.5e-10
Identity = 39/78 (50.00%), Postives = 50/78 (64.10%), Query Frame = 0


Query: 14 LSREQLCLIFRNHDGDRDGRLSFAELTEAFRYIGALLPPFRAFCALLHVDSDRDGFIFID 73
          L+ EQL  IFR HD +RDG LS AEL  AF+ +GAL+P  RAF  L   D++ DG  FI 
Sbjct: 13 LNEEQLREIFRQHDVNRDGLLSKAELKSAFKSLGALIPSLRAFLGLRQADANGDG--FIG 72

Query: 74 QKDFELLVNYAEERKYTV 92
          +++   LV YA +  YTV
Sbjct: 73 EEELNGLVKYASQLGYTV 88

[Close]

BLAST of Spg030254 vs. ExPASy TrEMBL
Match: A5AWP8 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_023174 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 2.5e-09
Identity = 37/86 (43.02%), Postives = 56/86 (65.12%), Query Frame = 0


Query: 6  ETYEQRPKLSREQLCLIFRNHDGDRDGRLSFAELTEAFRYIGALLPPFRAFCALLHVDSD 65
          +T   +  L  +QL  IF+ HD + DG+L+ AEL +AF+++GAL+P +RA  AL H D+D
Sbjct: 7  KTIPSKSPLPEQQLKKIFKQHDTNGDGQLNKAELKQAFQHLGALIPGWRAHRALHHADTD 66

Query: 66 RDGFIFIDQKDFELLVNYAEERKYTV 92
           DG   I +K+ + LV YA +  YT+
Sbjct: 67 GDG--CISEKEMKDLVEYAAKFGYTI 90

[Close]

BLAST of Spg030254 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 41.2 bits (95), Expect = 5.3e-04
Identity = 23/71 (32.39%), Postives = 38/71 (53.52%), Query Frame = 0


Query: 18  QLCLIFRNHDGDRDGRLSFAELTEAFRYIGALLPPFRAFCALLHVDSDRDGFIFIDQKDF 77
           +L  +F   D +RDGR+S  EL ++F  +G  L    A  A+   D+D DG +  ++   
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 78  ELLVNYAEERK 89
            + V+  EE+K
Sbjct: 103 LIKVDDEEEKK 113

[Close]

BLAST of Spg030254 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 41.2 bits (95), Expect = 5.3e-04
Identity = 23/71 (32.39%), Postives = 38/71 (53.52%), Query Frame = 0


Query: 18  QLCLIFRNHDGDRDGRLSFAELTEAFRYIGALLPPFRAFCALLHVDSDRDGFIFIDQKDF 77
           +L  +F   D +RDGR+S  EL ++F  +G  L    A  A+   D+D DG +  ++   
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 78  ELLVNYAEERK 89
            + V+  EE+K
Sbjct: 103 LIKVDDEEEKK 113

[Close]

BLAST of Spg030254 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 40.4 bits (93), Expect = 9.0e-04
Identity = 24/71 (33.80%), Postives = 37/71 (52.11%), Query Frame = 0


Query: 22 IFRNHDGDRDGRLSFAELTEAFRYIGALLPPFRAFCALLHVDSDRDGFIFIDQKDFELLV 81
          +F   D +RDGR+S  EL ++F+ +G  +    A  A+   D D DG   +D  +F LL+
Sbjct: 26 VFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDG--MLDINEFALLI 85

Query: 82 ----NYAEERK 89
               + EE K
Sbjct: 86 KGNDEFTEEEK 94

The following BLAST results are available for this feature:

BLAST of Spg030254 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7024248.1	4.9e-28	73.91	Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAA0064376.1	4.9e-28	72.83	putative calcium-binding protein CML27 [Cucumis melo var. makuwa] >TYK20211.1 pu...	[more]
KAG6591370.1	4.2e-27	73.33	Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KGN55185.1	2.7e-26	70.65	hypothetical protein Csa_012915 [Cucumis sativus]	[more]
KAG6600743.1	4.6e-26	69.57	Calcium-binding protein CML38, partial [Cucurbita argyrosperma subsp. sororia] >...	[more]

BLAST of Spg030254 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0DJV6	5.1e-04	37.74	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Spg030254 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VDB9	2.4e-28	72.83	Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0KZR6	1.3e-26	70.65	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639730 PE=4 SV=1	[more]
A0A6J1CS20	6.5e-10	47.50	calmodulin-like protein 5 OS=Momordica charantia OX=3673 GN=LOC111014054 PE=4 SV...	[more]
A0A5N6RQU3	8.5e-10	50.00	Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_018534 PE=4 SV=1	[more]
A5AWP8	2.5e-09	43.02	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_023174 PE=4 SV=1	[more]

BLAST of Spg030254 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G76650.1	5.3e-04	32.39	calmodulin-like 38	[more]
AT1G76650.3	5.3e-04	32.39	calmodulin-like 38	[more]
AT1G76640.1	9.0e-04	33.80	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.238.10		coord: 1..88 e-value: 4.4E-8 score: 35.2
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 27..39
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 14..49 score: 12.114401
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 16..80

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg030254.1	Spg030254.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding