Spg030252 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg030252
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationscaffold6: 14093805 .. 14096394 (+)
RNA-Seq ExpressionSpg030252
SyntenySpg030252
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACCAAAATAAAAAGTTAAAGAAATGGAAAAAAAATTCTAACCGAGTTTTAGAAGTTTAAATTGGATCGACTCAATTATCAAGTATTAATCATATTTTGTATGGGAAGTTTAAGGTTAAATTAGATCGACTCAAAAAAAAAAAAAAAAAAAACAAAAAAACAAAAAAATAGATACGACACGATACGAGTACAACCCTAAAACCTGTTCGTTCCTTTTCAAACTGTGAAGTGAGCGCTCAAAGAAAGCTGTCTAATAGAATCATCATTTCGCTGCAAATAGAGAAATCAAACAATCAAACTAGAATTCTTCCGGAATTGCAGCTTCCAAAGCTTCGTCCATGGCCGCGGCGTTGGCAATGTTCAAAATCTTGAGGGGTTCTTCTTCAGGCTGCACGGGAACGGCAAGAACAGAGACCGATGCATTTCCATTCTGTTTTCTGGCGTTGAGATTATATAGCGCGAGACGAAACTGCAACCGAAGAAACCTCTTCGCAAGGATTAGTCCTCTCGGTGCTCCTGAGCTTAGTGTAGTTCCGGTTCTTGATCAGTGGATTGAGGGGGAAGGCAGGAAGATCAAGGACTTTGAGCTTCGGAGAATCGTTCGCGACCTTCGTAATTGCCGGCGATATGGCCAAGCCCTTGAGGTGAGCGAAATCCATAAACAGACGCTAATTTCTGCTTGATTTTACCATTTCCCGTTAAAGGAATGCTTGTATTGTATTGTATATGAAGTCTGTTGGATGATGTAAAATTCTTTTGGATTTATTCCTCTGAATCTCAAATCTTTTTTTCGTGTTTTATTCCCATAATGCTTGATTGGTTGCTGAAAATATTGTGGAGGAAAGCAACGAACTGGAAATGTGCATTTTGAATTTTGAAGTAATCGCATGTGGTTAAGCTAATTCTGAGTTTACACGCTCACCTTCATTACAAAGTGATTCTATCAGTGGCAATGCATTAGTTGCAGTTTCACTAGAGGATGGCAAATTTTATGTTCTGTACCATTGTTGAATTCCTGTGTTTAGCTCTGCACTAGGTTGATGTCCTAAGCTTTTCTCATCCCATTTGATTTTTCATCAGTCCTAGATTTTATTTTTCAAATATCTATAAGATTCTGGATAGTTGATCATTTGTTCTTCTCTTGGATTATTCAAATTGAAGGTGTCTGAATGGATGAGTAGCAAGGGACTTTTTTCCCTTACAACAAGAGACTTTGCTGTACAGCTCGATTTGATTGGCCGAGTTCGGGGGCTGGATTCTGCAGAGAAGTATTTTGGCAGTGTTTCTAACCAACAGGAAATTGGTAAACTCTATGGTGCTCTTCTAAATTGTTATGTCAGGGAAGGCCTTGTAGATAAGTCTCTTTCCCATATGCAGAAGATGAAAGAGATGGGGTTTGCTTCCTCTCCCCTCTGCTACAATGATATAATGTGTCTGTATATGAACACTGGCCAGGTCGATAAAGTTCCGAATGTACTGTCTGAAATGAAGGAGAATGGTGTTCTTCCTGACAATTTTAGCTATAGAATTTGCATCAATTCTTATGGAGCTAGGTCTGATCTAATCAGTATGGAGAAGGTTTTGAAAGAAATGGAGAGTCAAACTCACATATCCATGGACTGGACTACTTATTCAATGGTTGCCAATTTTTTCATAAAAGCCAATATGCATGAGAAAGCAATGAATTACCTTCGGAAATGTGAGGACAAGGTCGATCAAGATGCACTCGGCCTCAATCATCTCATTTCACTCTACACCAGTCTGGGACGTAAAGACGAAGTAAGAAGATTGTGGGCTCTCCAGAAGAAGTGTAAGAAGCAAGTCAATAGGGATTATATAACCATGTTGGGTTCTTTGGTAAAGCTTGAGTTGCTTGAGGAAGCTGAGAACTTGCTGAAGGAGTGGGAGTCATCTTGCCAGTGTTATGATTTTCGAGTTCCGAACGTTCTTCTTATCGGATACTCACAAAAGGGGTTAATTGAAAGAGCTGAAAAGATGCTTCAAAACATCATCAGTGATGGGAGGATCCCACCACCAAATAGCTGGGGAATTATTGCAGCAGGGTACCTGGAGAAGCAGGAGCTGGAGAAAGCTTTCAAGTGCATGAAAGAAGCTGTAGCTGCACAAGAGCAGAACAAAGGGTGGAGGCCCAAACCTAGCGTTTTATCAAGCATTCTGCGATGGCTATCTGAAAATGGAAGATATGAGGAGCTAAAAGAGTTTTTGAACTCATTGAAGACTGTACCTTCCATGGATGGAAAACTAAGTGATGCCTTTGATAAGCTTCTGGAGACCTTGAAAAATGATGATGAAGTAACGACGATTGGCGAATTAGAGGTGAGGTGATTAAATGTTCTTATGAAATCACAACATTCTTTAAGAATTTGTAATATCAATTTGTAAACTTGCTGCCAATGTAGGAACATGTACATAAATTAAAGAGGAACTGTAGGGGTTACAATTTTCATTTACTTTTTATAGTCAGGTTTCAAGAATGTTTCTAAAGATTTTGTACCCTTGAAAAATGTACCGTTTAAGATACTGACAAGTTAGTTTTTATTAGGCAGGTATATGAACTTTAGGAAAAAC

mRNA sequence

ATGGCCGCGGCGTTGGCAATGTTCAAAATCTTGAGGGGTTCTTCTTCAGGCTGCACGGGAACGGCAAGAACAGAGACCGATGCATTTCCATTCTGTTTTCTGGCGTTGAGATTATATAGCGCGAGACGAAACTGCAACCGAAGAAACCTCTTCGCAAGGATTAGTCCTCTCGGTGCTCCTGAGCTTAGTGTAGTTCCGGTTCTTGATCAGTGGATTGAGGGGGAAGGCAGGAAGATCAAGGACTTTGAGCTTCGGAGAATCGTTCGCGACCTTCGTAATTGCCGGCGATATGGCCAAGCCCTTGAGGTGTCTGAATGGATGAGTAGCAAGGGACTTTTTTCCCTTACAACAAGAGACTTTGCTGTACAGCTCGATTTGATTGGCCGAGTTCGGGGGCTGGATTCTGCAGAGAAGTATTTTGGCAGTGTTTCTAACCAACAGGAAATTGGTAAACTCTATGGTGCTCTTCTAAATTGTTATGTCAGGGAAGGCCTTGTAGATAAGTCTCTTTCCCATATGCAGAAGATGAAAGAGATGGGGTTTGCTTCCTCTCCCCTCTGCTACAATGATATAATGTGTCTGTATATGAACACTGGCCAGGTCGATAAAGTTCCGAATGTACTGTCTGAAATGAAGGAGAATGGTGTTCTTCCTGACAATTTTAGCTATAGAATTTGCATCAATTCTTATGGAGCTAGGTCTGATCTAATCAGTATGGAGAAGGTTTTGAAAGAAATGGAGAGTCAAACTCACATATCCATGGACTGGACTACTTATTCAATGGTTGCCAATTTTTTCATAAAAGCCAATATGCATGAGAAAGCAATGAATTACCTTCGGAAATGTGAGGACAAGGTCGATCAAGATGCACTCGGCCTCAATCATCTCATTTCACTCTACACCAGTCTGGGACGTAAAGACGAAGTAAGAAGATTGTGGGCTCTCCAGAAGAAGTGTAAGAAGCAAGTCAATAGGGATTATATAACCATGTTGGGTTCTTTGGTAAAGCTTGAGTTGCTTGAGGAAGCTGAGAACTTGCTGAAGGAGTGGGAGTCATCTTGCCAGTGTTATGATTTTCGAGTTCCGAACGTTCTTCTTATCGGATACTCACAAAAGGGGTTAATTGAAAGAGCTGAAAAGATGCTTCAAAACATCATCAGTGATGGGAGGATCCCACCACCAAATAGCTGGGGAATTATTGCAGCAGGGTACCTGGAGAAGCAGGAGCTGGAGAAAGCTTTCAAGTGCATGAAAGAAGCTGTAGCTGCACAAGAGCAGAACAAAGGGTGGAGGCCCAAACCTAGCGTTTTATCAAGCATTCTGCGATGGCTATCTGAAAATGGAAGATATGAGGAGCTAAAAGAGTTTTTGAACTCATTGAAGACTGTACCTTCCATGGATGGAAAACTAAGTGATGCCTTTGATAAGCTTCTGGAGACCTTGAAAAATGATGATGAAGTAACGACGATTGGCGAATTAGAGGTGAGGTGA

Coding sequence (CDS)

ATGGCCGCGGCGTTGGCAATGTTCAAAATCTTGAGGGGTTCTTCTTCAGGCTGCACGGGAACGGCAAGAACAGAGACCGATGCATTTCCATTCTGTTTTCTGGCGTTGAGATTATATAGCGCGAGACGAAACTGCAACCGAAGAAACCTCTTCGCAAGGATTAGTCCTCTCGGTGCTCCTGAGCTTAGTGTAGTTCCGGTTCTTGATCAGTGGATTGAGGGGGAAGGCAGGAAGATCAAGGACTTTGAGCTTCGGAGAATCGTTCGCGACCTTCGTAATTGCCGGCGATATGGCCAAGCCCTTGAGGTGTCTGAATGGATGAGTAGCAAGGGACTTTTTTCCCTTACAACAAGAGACTTTGCTGTACAGCTCGATTTGATTGGCCGAGTTCGGGGGCTGGATTCTGCAGAGAAGTATTTTGGCAGTGTTTCTAACCAACAGGAAATTGGTAAACTCTATGGTGCTCTTCTAAATTGTTATGTCAGGGAAGGCCTTGTAGATAAGTCTCTTTCCCATATGCAGAAGATGAAAGAGATGGGGTTTGCTTCCTCTCCCCTCTGCTACAATGATATAATGTGTCTGTATATGAACACTGGCCAGGTCGATAAAGTTCCGAATGTACTGTCTGAAATGAAGGAGAATGGTGTTCTTCCTGACAATTTTAGCTATAGAATTTGCATCAATTCTTATGGAGCTAGGTCTGATCTAATCAGTATGGAGAAGGTTTTGAAAGAAATGGAGAGTCAAACTCACATATCCATGGACTGGACTACTTATTCAATGGTTGCCAATTTTTTCATAAAAGCCAATATGCATGAGAAAGCAATGAATTACCTTCGGAAATGTGAGGACAAGGTCGATCAAGATGCACTCGGCCTCAATCATCTCATTTCACTCTACACCAGTCTGGGACGTAAAGACGAAGTAAGAAGATTGTGGGCTCTCCAGAAGAAGTGTAAGAAGCAAGTCAATAGGGATTATATAACCATGTTGGGTTCTTTGGTAAAGCTTGAGTTGCTTGAGGAAGCTGAGAACTTGCTGAAGGAGTGGGAGTCATCTTGCCAGTGTTATGATTTTCGAGTTCCGAACGTTCTTCTTATCGGATACTCACAAAAGGGGTTAATTGAAAGAGCTGAAAAGATGCTTCAAAACATCATCAGTGATGGGAGGATCCCACCACCAAATAGCTGGGGAATTATTGCAGCAGGGTACCTGGAGAAGCAGGAGCTGGAGAAAGCTTTCAAGTGCATGAAAGAAGCTGTAGCTGCACAAGAGCAGAACAAAGGGTGGAGGCCCAAACCTAGCGTTTTATCAAGCATTCTGCGATGGCTATCTGAAAATGGAAGATATGAGGAGCTAAAAGAGTTTTTGAACTCATTGAAGACTGTACCTTCCATGGATGGAAAACTAAGTGATGCCTTTGATAAGCTTCTGGAGACCTTGAAAAATGATGATGAAGTAACGACGATTGGCGAATTAGAGGTGAGGTGA

Protein sequence

MAAALAMFKILRGSSSGCTGTARTETDAFPFCFLALRLYSARRNCNRRNLFARISPLGAPELSVVPVLDQWIEGEGRKIKDFELRRIVRDLRNCRRYGQALEVSEWMSSKGLFSLTTRDFAVQLDLIGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLVDKSLSHMQKMKEMGFASSPLCYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRICINSYGARSDLISMEKVLKEMESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDKVDQDALGLNHLISLYTSLGRKDEVRRLWALQKKCKKQVNRDYITMLGSLVKLELLEEAENLLKEWESSCQCYDFRVPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQELEKAFKCMKEAVAAQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTVPSMDGKLSDAFDKLLETLKNDDEVTTIGELEVR
Homology
BLAST of Spg030252 vs. NCBI nr
Match: KAG6600732.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 892.1 bits (2304), Expect = 2.2e-255
Identity = 442/488 (90.57%), Postives = 464/488 (95.08%), Query Frame = 0

Query: 1   MAAALAMFKILRGSSSGCTGTARTETDAFPFCFLALRLYSARRNCNRRNLFARISPLGAP 60
           +AAALAMFKILR  SSG T TARTETDA  FCF+ALRLYSARR CNRRNLFARISPLG+P
Sbjct: 49  LAAALAMFKILRSFSSGFTRTARTETDA--FCFVALRLYSARRTCNRRNLFARISPLGSP 108

Query: 61  ELSVVPVLDQWIEGEGRKIKDFELRRIVRDLRNCRRYGQALEVSEWMSSKGLFSLTTRDF 120
           ELSVVP+LDQWI+ EGR IKDFE+RRIVRDLRNCRRYGQAL+VSEWM SKGLFS TTRDF
Sbjct: 109 ELSVVPILDQWIQ-EGRMIKDFEMRRIVRDLRNCRRYGQALQVSEWMRSKGLFSFTTRDF 168

Query: 121 AVQLDLIGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLVDKSLSHMQKMKEMG 180
           AVQLDLIGRVRG+DSAEKYFGSVSNQ+EIGKLYGALLNCYVREGLVDK+LSHMQKMKEMG
Sbjct: 169 AVQLDLIGRVRGIDSAEKYFGSVSNQEEIGKLYGALLNCYVREGLVDKALSHMQKMKEMG 228

Query: 181 FASSPLCYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRICINSYGARSDLISME 240
           FASSPLCYNDIMCLY+NTGQVDKVPNVLSEMK NGVLPDN+SYRICI+SYGARSDLI M 
Sbjct: 229 FASSPLCYNDIMCLYLNTGQVDKVPNVLSEMKVNGVLPDNYSYRICISSYGARSDLIGML 288

Query: 241 KVLKEMESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDKVDQDALGLNHLISLY 300
           KVLKEMESQTHISMDWTTYSMVANFFIKA MHEKAM+YLRKCEDKV+QDALG NHLISLY
Sbjct: 289 KVLKEMESQTHISMDWTTYSMVANFFIKAGMHEKAMSYLRKCEDKVNQDALGFNHLISLY 348

Query: 301 TSLGRKDEVRRLWALQKKCKKQVNRDYITMLGSLVKLELLEEAENLLKEWESSCQCYDFR 360
           TSLGRKDEV RLWALQKKCKKQVNRDYITMLG LVKLE LEEAE L+KEWESSC+CYDFR
Sbjct: 349 TSLGRKDEVMRLWALQKKCKKQVNRDYITMLGCLVKLEFLEEAEKLVKEWESSCECYDFR 408

Query: 361 VPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQELEKAFKCMKEA 420
           VPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQ  E+AFKCMKEA
Sbjct: 409 VPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQNPERAFKCMKEA 468

Query: 421 VAAQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTVPSMDGKLSDAFDKLLET 480
           VA QEQNKGWRPKPSVLSSILRWLSENGRYEELKEFL+SLKTVPSMDGKLS+AFD+LLET
Sbjct: 469 VAVQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLSSLKTVPSMDGKLSNAFDELLET 528

Query: 481 LKNDDEVT 489
           LKN+DE T
Sbjct: 529 LKNNDETT 533

BLAST of Spg030252 vs. NCBI nr
Match: XP_023545982.1 (pentatricopeptide repeat-containing protein At4g21705, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 889.0 bits (2296), Expect = 1.8e-254
Identity = 440/488 (90.16%), Postives = 464/488 (95.08%), Query Frame = 0

Query: 1   MAAALAMFKILRGSSSGCTGTARTETDAFPFCFLALRLYSARRNCNRRNLFARISPLGAP 60
           +AAALAMFKILR  SSG T TARTETDA  FCF+ALRLYSARR CNRRNLFARISPLG+P
Sbjct: 38  LAAALAMFKILRSFSSGFTRTARTETDA--FCFVALRLYSARRTCNRRNLFARISPLGSP 97

Query: 61  ELSVVPVLDQWIEGEGRKIKDFELRRIVRDLRNCRRYGQALEVSEWMSSKGLFSLTTRDF 120
           ELSVVP+LDQWI+ EGR IKDFE+RRIVRDLRNCRRYGQALEVSEWM SKGLFS TTRDF
Sbjct: 98  ELSVVPILDQWIQ-EGRMIKDFEMRRIVRDLRNCRRYGQALEVSEWMRSKGLFSFTTRDF 157

Query: 121 AVQLDLIGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLVDKSLSHMQKMKEMG 180
           AVQLDLIGRV+GLDSAEKYF SVSNQ+EIGKLYGALLNCYVREGLVDK+LSHMQKMKEMG
Sbjct: 158 AVQLDLIGRVQGLDSAEKYFSSVSNQEEIGKLYGALLNCYVREGLVDKALSHMQKMKEMG 217

Query: 181 FASSPLCYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRICINSYGARSDLISME 240
           FASSPLCYNDIMCLY+NTGQVDKVPNVLSEMKENGVLPDN+SYRICI+SYGARSDLI M 
Sbjct: 218 FASSPLCYNDIMCLYLNTGQVDKVPNVLSEMKENGVLPDNYSYRICISSYGARSDLIGML 277

Query: 241 KVLKEMESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDKVDQDALGLNHLISLY 300
           KVL+EMESQTHISMDWTTYSMVANFFIKA MHE+AM+YLRKCEDKV+QDALG NHLISLY
Sbjct: 278 KVLREMESQTHISMDWTTYSMVANFFIKAGMHEQAMSYLRKCEDKVNQDALGFNHLISLY 337

Query: 301 TSLGRKDEVRRLWALQKKCKKQVNRDYITMLGSLVKLELLEEAENLLKEWESSCQCYDFR 360
           TSLGRKDEV RLWALQKKCKKQVNRDYITMLG LVKLE LEEAE L+KEWESSC+CYDFR
Sbjct: 338 TSLGRKDEVMRLWALQKKCKKQVNRDYITMLGCLVKLEFLEEAEKLVKEWESSCECYDFR 397

Query: 361 VPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQELEKAFKCMKEA 420
           VPNVLLIGYSQ+GLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQ  E+AFKCMKEA
Sbjct: 398 VPNVLLIGYSQRGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQNPERAFKCMKEA 457

Query: 421 VAAQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTVPSMDGKLSDAFDKLLET 480
           VA QEQNKGWRPKPSVLSSILRWLSENGRYEELKEFL+SLKTVPSMDGKLS+AFD+LLET
Sbjct: 458 VAVQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLSSLKTVPSMDGKLSNAFDELLET 517

Query: 481 LKNDDEVT 489
           LKN+DE T
Sbjct: 518 LKNNDETT 522

BLAST of Spg030252 vs. NCBI nr
Match: XP_022989754.1 (pentatricopeptide repeat-containing protein At4g21705, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 887.9 bits (2293), Expect = 4.1e-254
Identity = 441/488 (90.37%), Postives = 463/488 (94.88%), Query Frame = 0

Query: 1   MAAALAMFKILRGSSSGCTGTARTETDAFPFCFLALRLYSARRNCNRRNLFARISPLGAP 60
           +AAALAMFKILR  SSG T TARTETDA  FCF+ALRLYSARR CNRRNLFARISPLG+P
Sbjct: 45  LAAALAMFKILRSFSSGFTRTARTETDA--FCFVALRLYSARRTCNRRNLFARISPLGSP 104

Query: 61  ELSVVPVLDQWIEGEGRKIKDFELRRIVRDLRNCRRYGQALEVSEWMSSKGLFSLTTRDF 120
           ELSVVP+LDQWI+ EGR IKDFELRRIVRDLRNCRRYGQALEVSEWM SKGLFS TTRDF
Sbjct: 105 ELSVVPILDQWIQ-EGRMIKDFELRRIVRDLRNCRRYGQALEVSEWMRSKGLFSFTTRDF 164

Query: 121 AVQLDLIGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLVDKSLSHMQKMKEMG 180
           AVQLDLIGRV+GLDSAEKYF SVSNQ+E+GKLYGALLNCYVREGLVDK+LSHMQKMKEMG
Sbjct: 165 AVQLDLIGRVQGLDSAEKYFSSVSNQEEMGKLYGALLNCYVREGLVDKALSHMQKMKEMG 224

Query: 181 FASSPLCYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRICINSYGARSDLISME 240
           FASSPLCYNDIMCLY+NTGQVDKVPNVLSEMKENGVLPDN+SYRICI+SYGARSDLI M 
Sbjct: 225 FASSPLCYNDIMCLYLNTGQVDKVPNVLSEMKENGVLPDNYSYRICISSYGARSDLIGML 284

Query: 241 KVLKEMESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDKVDQDALGLNHLISLY 300
           KVLKEMESQTHISMDWTTYSMVANFFIKA MHEKAM+YLRKCEDKV+QDALG NHLISLY
Sbjct: 285 KVLKEMESQTHISMDWTTYSMVANFFIKAGMHEKAMSYLRKCEDKVNQDALGFNHLISLY 344

Query: 301 TSLGRKDEVRRLWALQKKCKKQVNRDYITMLGSLVKLELLEEAENLLKEWESSCQCYDFR 360
           TSLG KDEV RLWALQKKCKKQVNRDYITMLG LVKLE LEEAE L+KEW SSC+CYDFR
Sbjct: 345 TSLGCKDEVMRLWALQKKCKKQVNRDYITMLGCLVKLEFLEEAEKLVKEWVSSCECYDFR 404

Query: 361 VPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQELEKAFKCMKEA 420
           VPNVLLIGYS++GLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQ LEKAFKCMKEA
Sbjct: 405 VPNVLLIGYSKRGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQNLEKAFKCMKEA 464

Query: 421 VAAQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTVPSMDGKLSDAFDKLLET 480
           VA QEQNKGWRPKPSVLSSILRWLSENGRYEELKEFL+SLKTVPSMDGKLS+AFD+LLET
Sbjct: 465 VAVQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLSSLKTVPSMDGKLSNAFDELLET 524

Query: 481 LKNDDEVT 489
           LKN+DE T
Sbjct: 525 LKNNDETT 529

BLAST of Spg030252 vs. NCBI nr
Match: XP_022942045.1 (pentatricopeptide repeat-containing protein At4g21705, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 885.2 bits (2286), Expect = 2.6e-253
Identity = 437/488 (89.55%), Postives = 463/488 (94.88%), Query Frame = 0

Query: 1   MAAALAMFKILRGSSSGCTGTARTETDAFPFCFLALRLYSARRNCNRRNLFARISPLGAP 60
           +AAALAMFKILR  SSG T TARTETDA  FCF+ALRLYSARR CNRRNLFARISPLG+P
Sbjct: 49  LAAALAMFKILRSFSSGFTRTARTETDA--FCFVALRLYSARRTCNRRNLFARISPLGSP 108

Query: 61  ELSVVPVLDQWIEGEGRKIKDFELRRIVRDLRNCRRYGQALEVSEWMSSKGLFSLTTRDF 120
           ELSVVP+LDQWI+ EGR IKDFE+RRIVRDLRNCRRYGQAL+VSEWM SKGLFS TTRDF
Sbjct: 109 ELSVVPILDQWIQ-EGRMIKDFEMRRIVRDLRNCRRYGQALQVSEWMRSKGLFSFTTRDF 168

Query: 121 AVQLDLIGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLVDKSLSHMQKMKEMG 180
           AVQLDLIGRVRG+DSAEKYF SVSNQ+EIGKLYGALLNCYVREGLVDK+LSHMQKMKEMG
Sbjct: 169 AVQLDLIGRVRGIDSAEKYFSSVSNQEEIGKLYGALLNCYVREGLVDKALSHMQKMKEMG 228

Query: 181 FASSPLCYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRICINSYGARSDLISME 240
           FASSPLCYNDIMCLY+NTGQVDKVPNVLSEMK+NGVLPDN+SYRICI+SYGARSDLI M 
Sbjct: 229 FASSPLCYNDIMCLYLNTGQVDKVPNVLSEMKDNGVLPDNYSYRICISSYGARSDLIGML 288

Query: 241 KVLKEMESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDKVDQDALGLNHLISLY 300
           KVLKEMESQTHISMDWTTYSMVANFFIKA MHE+AM+YLRKCEDKV+QDALG NHLISLY
Sbjct: 289 KVLKEMESQTHISMDWTTYSMVANFFIKAGMHEQAMSYLRKCEDKVNQDALGFNHLISLY 348

Query: 301 TSLGRKDEVRRLWALQKKCKKQVNRDYITMLGSLVKLELLEEAENLLKEWESSCQCYDFR 360
           TSLGRKDEV RLWALQKKCKKQVNRDYITMLG LVKLE LEEAE L++EWESSC+CYDFR
Sbjct: 349 TSLGRKDEVMRLWALQKKCKKQVNRDYITMLGCLVKLEFLEEAEKLVEEWESSCECYDFR 408

Query: 361 VPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQELEKAFKCMKEA 420
           VPNVLLIGYSQ+GLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQ  E+AFKCMKEA
Sbjct: 409 VPNVLLIGYSQRGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQNPERAFKCMKEA 468

Query: 421 VAAQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTVPSMDGKLSDAFDKLLET 480
           VA QEQNKGWRPKPSVLSSILRWLSENGRYEELKEFL+SLK VPSMDGKLS+AFD+LLET
Sbjct: 469 VAVQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLSSLKAVPSMDGKLSNAFDELLET 528

Query: 481 LKNDDEVT 489
           LKN+DE T
Sbjct: 529 LKNNDETT 533

BLAST of Spg030252 vs. NCBI nr
Match: KAG7031372.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 884.8 bits (2285), Expect = 3.5e-253
Identity = 436/482 (90.46%), Postives = 459/482 (95.23%), Query Frame = 0

Query: 7   MFKILRGSSSGCTGTARTETDAFPFCFLALRLYSARRNCNRRNLFARISPLGAPELSVVP 66
           MFKILR  SSG T TARTETDA  FCF+ALRLYSARR CNRRNLFARISPLG+PELSVVP
Sbjct: 1   MFKILRSFSSGFTRTARTETDA--FCFVALRLYSARRTCNRRNLFARISPLGSPELSVVP 60

Query: 67  VLDQWIEGEGRKIKDFELRRIVRDLRNCRRYGQALEVSEWMSSKGLFSLTTRDFAVQLDL 126
           +LDQWI+ EGR IKDFE+RRIVRDLRNCRRYGQAL+VSEWM SKGLFS TTRDFAVQLDL
Sbjct: 61  ILDQWIQ-EGRMIKDFEMRRIVRDLRNCRRYGQALQVSEWMRSKGLFSFTTRDFAVQLDL 120

Query: 127 IGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLVDKSLSHMQKMKEMGFASSPL 186
           IGRVRG+DSAEKYFGSVSNQ+EIGKLYGALLNCYVREGLVDK+LSHMQKMKEMGFASSPL
Sbjct: 121 IGRVRGIDSAEKYFGSVSNQEEIGKLYGALLNCYVREGLVDKALSHMQKMKEMGFASSPL 180

Query: 187 CYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRICINSYGARSDLISMEKVLKEM 246
           CYNDIMCLY+NTGQVDKVPNVLSEMK+NGVLPDN+SYRICI+SYGARSDLI M KVLKEM
Sbjct: 181 CYNDIMCLYLNTGQVDKVPNVLSEMKDNGVLPDNYSYRICISSYGARSDLIGMLKVLKEM 240

Query: 247 ESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDKVDQDALGLNHLISLYTSLGRK 306
           ESQTHISMDWTTYSMVANFFIKA MHEKAM+YLRKCEDKV+QDALG NHLISLYTSLGRK
Sbjct: 241 ESQTHISMDWTTYSMVANFFIKAGMHEKAMSYLRKCEDKVNQDALGFNHLISLYTSLGRK 300

Query: 307 DEVRRLWALQKKCKKQVNRDYITMLGSLVKLELLEEAENLLKEWESSCQCYDFRVPNVLL 366
           DEV RLWALQKKCKKQVNRDYITMLG LVKLE LEEAE L++EWESSC+CYDFRVPNVLL
Sbjct: 301 DEVMRLWALQKKCKKQVNRDYITMLGCLVKLEFLEEAEKLVEEWESSCECYDFRVPNVLL 360

Query: 367 IGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQELEKAFKCMKEAVAAQEQ 426
           IGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQ  E+AFKCMKEAVA QEQ
Sbjct: 361 IGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQNPERAFKCMKEAVAVQEQ 420

Query: 427 NKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTVPSMDGKLSDAFDKLLETLKNDDE 486
           NKGWRPKPSVLSSILRWLSENGRYEELKEFL+SLKTVPSMDGKLS+AFD+LLETLKN+DE
Sbjct: 421 NKGWRPKPSVLSSILRWLSENGRYEELKEFLSSLKTVPSMDGKLSNAFDELLETLKNNDE 479

Query: 487 VT 489
            T
Sbjct: 481 TT 479

BLAST of Spg030252 vs. ExPASy Swiss-Prot
Match: Q84JR3 (Pentatricopeptide repeat-containing protein At4g21705, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g21705 PE=2 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 4.1e-116
Identity = 217/479 (45.30%), Postives = 312/479 (65.14%), Query Frame = 0

Query: 30  PFCFLALRLYSARRNCNRRNLFARISPLGAPELSVVPVLDQWIEGEGRKIKDFELRRIVR 89
           P   +A R Y   R   +  L+++ISPLG P+ SV P L  W++  G+K+   EL RIV 
Sbjct: 8   PANLIASRYYYTNR-VKKTTLYSKISPLGDPKSSVYPELQNWVQC-GKKVSVAELIRIVH 67

Query: 90  DLRNCRRYGQALEVSEWMSSKGLFSLTTRDFAVQLDLIGRVRGLDSAEKYFGSVSNQQEI 149
           DLR  +R+  ALEVS+WM+  G+   +  + AV LDLIGRV G  +AE+YF ++  Q + 
Sbjct: 68  DLRRRKRFLHALEVSKWMNETGVCVFSPTEHAVHLDLIGRVYGFVTAEEYFENLKEQYKN 127

Query: 150 GKLYGALLNCYVREGLVDKSLSHMQKMKEMGFASSPLCYNDIMCLYMNTGQVDKVPNVLS 209
            K YGALLNCYVR+  V+KSL H +KMKEMGF +S L YN+IMCLY N GQ +KVP VL 
Sbjct: 128 DKTYGALLNCYVRQQNVEKSLLHFEKMKEMGFVTSSLTYNNIMCLYTNIGQHEKVPKVLE 187

Query: 210 EMKENGVLPDNFSYRICINSYGARSDLISMEKVLKEMESQTHISMDWTTYSMVANFFIKA 269
           EMKE  V PDN+SYRICIN++GA  DL  +   L++ME +  I+MDW TY++ A F+I  
Sbjct: 188 EMKEENVAPDNYSYRICINAFGAMYDLERIGGTLRDMERRQDITMDWNTYAVAAKFYIDG 247

Query: 270 NMHEKAMNYLRKCEDKVD-QDALGLNHLISLYTSLGRKDEVRRLWALQKK-CKKQVNRDY 329
              ++A+  L+  E++++ +D  G NHLI+LY  LG+K EV RLW L+K  CK+++N+DY
Sbjct: 248 GDCDRAVELLKMSENRLEKKDGEGYNHLITLYARLGKKIEVLRLWDLEKDVCKRRINQDY 307

Query: 330 ITMLGSLVKLELLEEAENLLKEWESSCQCYDFRVPNVLLIGYSQKGLIERAEKMLQNIIS 389
           +T+L SLVK++ L EAE +L EW+SS  CYDFRVPN ++ GY  K + E+AE ML+++  
Sbjct: 308 LTVLQSLVKIDALVEAEEVLTEWKSSGNCYDFRVPNTVIRGYIGKSMEEKAEAMLEDLAR 367

Query: 390 DGRIPPPNSWGIIAAGYLEKQELEKAFKCMKEAVAAQEQNKGWRPKPSVLSSILRWLSEN 449
            G+   P SW ++A  Y EK  LE AFKCMK A+  +  ++ WRP  ++++S+L W+ + 
Sbjct: 368 RGKATTPESWELVATAYAEKGTLENAFKCMKTALGVEVGSRKWRPGLTLVTSVLSWVGDE 427

Query: 450 GRYEELKEFLNSLKTVPSMDGKLSDA------------FDKLLETLKND----DEVTTI 491
           G  +E++ F+ SL+    ++ ++  A             D LL+ +K+D    DE TT+
Sbjct: 428 GSLKEVESFVASLRNCIGVNKQMYHALVKADIREGGRNIDTLLQRMKDDKIEIDEETTV 484

BLAST of Spg030252 vs. ExPASy Swiss-Prot
Match: Q9SKU6 (Pentatricopeptide repeat-containing protein At2g20710, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g20710 PE=2 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 9.3e-76
Identity = 153/428 (35.75%), Postives = 252/428 (58.88%), Query Frame = 0

Query: 53  RISPLGAPELSVVPVLDQWIEGEGRKIKDFELRRIVRDLRNCRRYGQALEVSEWMSSKGL 112
           R++  G P  S++ VLD W++ +G  +K  EL  I++ LR   R+  AL++S+WMS   +
Sbjct: 43  RVARSGDPSASIIKVLDGWLD-QGNLVKTSELHSIIKMLRKFSRFSHALQISDWMSEHRV 102

Query: 113 FSLTTRDFAVQLDLIGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLVDKSLSH 172
             ++  D A++LDLI +V GL  AEK+F ++  ++    LYGALLNCY  + ++ K+   
Sbjct: 103 HEISEGDVAIRLDLIAKVGGLGEAEKFFETIPMERRNYHLYGALLNCYASKKVLHKAEQV 162

Query: 173 MQKMKEMGFASSPLCYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRICINSYGA 232
            Q+MKE+GF    L YN ++ LY+ TG+   V  +L EM++  V PD F+    +++Y  
Sbjct: 163 FQEMKELGFLKGCLPYNVMLNLYVRTGKYTMVEKLLREMEDETVKPDIFTVNTRLHAYSV 222

Query: 233 RSDLISMEKVLKEMESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDKVDQDAL- 292
            SD+  MEK L   E+   + +DW TY+  AN +IKA + EKA+  LRK E  V+     
Sbjct: 223 VSDVEGMEKFLMRCEADQGLHLDWRTYADTANGYIKAGLTEKALEMLRKSEQMVNAQKRK 282

Query: 293 -GLNHLISLYTSLGRKDEVRRLWALQKKCKKQVNRDYITMLGSLVKLELLEEAENLLKEW 352
                L+S Y + G+K+EV RLW+L K+     N  YI+++ +L+K++ +EE E +++EW
Sbjct: 283 HAYEVLMSFYGAAGKKEEVYRLWSLYKELDGFYNTGYISVISALLKMDDIEEVEKIMEEW 342

Query: 353 ESSCQCYDFRVPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQEL 412
           E+    +D R+P++L+ GY +KG++E+AE+++  ++   R+   ++W  +A GY    ++
Sbjct: 343 EAGHSLFDIRIPHLLITGYCKKGMMEKAEEVVNILVQKWRVEDTSTWERLALGYKMAGKM 402

Query: 413 EKAFKCMKEAVAAQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTVPSMDGKL 472
           EKA +  K A+  +    GWRP   VL S + +L      E L++ L  L    S  G +
Sbjct: 403 EKAVEKWKRAI--EVSKPGWRPHQVVLMSCVDYLEGQRDMEGLRKILRLL----SERGHI 461

Query: 473 SDAFDKLL 479
           S  +D+LL
Sbjct: 463 S--YDQLL 461

BLAST of Spg030252 vs. ExPASy Swiss-Prot
Match: Q8LPS6 (Pentatricopeptide repeat-containing protein At1g02150 OS=Arabidopsis thaliana OX=3702 GN=At1g02150 PE=2 SV=2)

HSP 1 Score: 273.1 bits (697), Expect = 6.2e-72
Identity = 157/449 (34.97%), Postives = 257/449 (57.24%), Query Frame = 0

Query: 50  LFARISPLGAPELSVVPVLDQWIEGEGRKIKDFELRRIVRDLRNCRRYGQALEVSEWMSS 109
           ++ +IS +  PEL    VL+QW E  GRK+  +EL R+V++LR  +R  QALEV +WM++
Sbjct: 69  IYKKISLMEKPELGAASVLNQW-EKAGRKLTKWELCRVVKELRKYKRANQALEVYDWMNN 128

Query: 110 KG-LFSLTTRDFAVQLDLIGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLVDK 169
           +G  F L+  D A+QLDLIG+VRG+  AE++F  +    +  ++YG+LLN YVR    +K
Sbjct: 129 RGERFRLSASDAAIQLDLIGKVRGIPDAEEFFLQLPENFKDRRVYGSLLNAYVRAKSREK 188

Query: 170 SLSHMQKMKEMGFASSPLCYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRICIN 229
           + + +  M++ G+A  PL +N +M LYMN  + DKV  ++ EMK+  +  D +SY I ++
Sbjct: 189 AEALLNTMRDKGYALHPLPFNVMMTLYMNLREYDKVDAMVFEMKQKDIRLDIYSYNIWLS 248

Query: 230 SYGARSDLISMEKVLKEMESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDKV-D 289
           S G+   +  ME V ++M+S   I  +WTT+S +A  +IK    EKA + LRK E ++  
Sbjct: 249 SCGSLGSVEKMELVYQQMKSDVSIYPNWTTFSTMATMYIKMGETEKAEDALRKVEARITG 308

Query: 290 QDALGLNHLISLYTSLGRKDEVRRLWALQKKCKKQV-NRDYITMLGSLVKLELLEEAENL 349
           ++ +  ++L+SLY SLG K E+ R+W + K     + N  Y  ++ SLV++  +E AE +
Sbjct: 309 RNRIPYHYLLSLYGSLGNKKELYRVWHVYKSVVPSIPNLGYHALVSSLVRMGDIEGAEKV 368

Query: 350 LKEWESSCQCYDFRVPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLE 409
            +EW      YD R+PN+L+  Y +   +E AE +  +++  G  P  ++W I+A G+  
Sbjct: 369 YEEWLPVKSSYDPRIPNLLMNAYVKNDQLETAEGLFDHMVEMGGKPSSSTWEILAVGHTR 428

Query: 410 KQELEKAFKCMKEAVAAQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTVPSM 469
           K+ + +A  C++ A +A E +  WRPK  +LS   +   E       +  L  L+     
Sbjct: 429 KRCISEALTCLRNAFSA-EGSSNWRPKVLMLSGFFKLCEEESDVTSKEAVLELLR----- 488

Query: 470 DGKLSDAFDKLLETLKNDDEVTTIGELEV 496
             +  D  DK    L + DE  T+   E+
Sbjct: 489 --QSGDLEDKSYLALIDVDENRTVNNSEI 508

BLAST of Spg030252 vs. ExPASy Swiss-Prot
Match: O22714 (Pentatricopeptide repeat-containing protein At1g60770 OS=Arabidopsis thaliana OX=3702 GN=At1g60770 PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 3.5e-67
Identity = 145/458 (31.66%), Postives = 251/458 (54.80%), Query Frame = 0

Query: 34  LALRLYSARRNCNRRN--------LFARISPLGAPELSVVPVLDQWIEGEGRKIKDFELR 93
           +A+R  S  R+  +R+        L+ R+   G  E+ V   L+Q+++G     K +E+ 
Sbjct: 1   MAMRHLSRSRDVTKRSTKKYIEEPLYNRLFKDGGTEVKVRQQLNQFLKGTKHVFK-WEVG 60

Query: 94  RIVRDLRNCRRYGQALEVSEWMSSKGLFSLTTRDFAVQLDLIGRVRGLDSAEKYFGSVSN 153
             ++ LRN   Y  AL++SE M  +G+ + T  D A+ LDL+ + R + + E YF  +  
Sbjct: 61  DTIKKLRNRGLYYPALKLSEVMEERGM-NKTVSDQAIHLDLVAKAREITAGENYFVDLPE 120

Query: 154 QQEIGKLYGALLNCYVREGLVDKSLSHMQKMKEMGFASSPLCYNDIMCLYMNTGQVDKVP 213
             +    YG+LLNCY +E L +K+   + KMKE+    S + YN +M LY  TG+ +KVP
Sbjct: 121 TSKTELTYGSLLNCYCKELLTEKAEGLLNKMKELNITPSSMSYNSLMTLYTKTGETEKVP 180

Query: 214 NVLSEMKENGVLPDNFSYRICINSYGARSDLISMEKVLKEMESQTHISMDWTTYSMVANF 273
            ++ E+K   V+PD+++Y + + +  A +D+  +E+V++EM     ++ DWTTYS +A+ 
Sbjct: 181 AMIQELKAENVMPDSYTYNVWMRALAATNDISGVERVIEEMNRDGRVAPDWTTYSNMASI 240

Query: 274 FIKANMHEKAMNYLRKCEDK-VDQDALGLNHLISLYTSLGRKDEVRRLW-ALQKKCKKQV 333
           ++ A + +KA   L++ E K   +D      LI+LY  LG+  EV R+W +L+    K  
Sbjct: 241 YVDAGLSQKAEKALQELEMKNTQRDFTAYQFLITLYGRLGKLTEVYRIWRSLRLAIPKTS 300

Query: 334 NRDYITMLGSLVKLELLEEAENLLKEWESSCQCYDFRVPNVLLIGYSQKGLIERAEKMLQ 393
           N  Y+ M+  LVKL  L  AE L KEW+++C  YD R+ NVL+  Y+Q+GLI++A ++ +
Sbjct: 301 NVAYLNMIQVLVKLNDLPGAETLFKEWQANCSTYDIRIVNVLIGAYAQEGLIQKANELKE 360

Query: 394 NIISDGRIPPPNSWGIIAAGYLEKQELEKAFKCMKEAVAAQEQNKG-WRPKPSVLSSILR 453
                G      +W I    Y++  ++ +A +CM +AV+  + + G W P P  + +++ 
Sbjct: 361 KAPRRGGKLNAKTWEIFMDYYVKSGDMARALECMSKAVSIGKGDGGKWLPSPETVRALMS 420

Query: 454 WLSENGRYEELKEFLNSLKTVPSMDGKLSDAFDKLLET 481
           +  +       +  L  LK     D   ++ F+ L+ T
Sbjct: 421 YFEQKKDVNGAENLLEILKN--GTDNIGAEIFEPLIRT 454

BLAST of Spg030252 vs. ExPASy Swiss-Prot
Match: Q9SY07 (Pentatricopeptide repeat-containing protein At4g02820, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g02820 PE=2 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 4.5e-62
Identity = 147/449 (32.74%), Postives = 244/449 (54.34%), Query Frame = 0

Query: 47  RRNLFARISPLGAPELSVVPVLDQWIEGEGRKIKDFELRRIVRDLRNCRRYGQALEVSEW 106
           R  L  R+  L   + S V  + +W E EG  ++ +EL RIVR+LR  +RY  ALE+ EW
Sbjct: 60  RDTLGGRLLSLVYTKRSAVVTIRKWKE-EGHSVRKYELNRIVRELRKIKRYKHALEICEW 119

Query: 107 MSSKGLFSLTTRDFAVQLDLIGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLV 166
           M  +    L   D+AV LDLI ++RGL+SAEK+F  + +Q        +LL+ YV+  L 
Sbjct: 120 MVVQEDIKLQAGDYAVHLDLISKIRGLNSAEKFFEDMPDQMRGHAACTSLLHSYVQNKLS 179

Query: 167 DKSLSHMQKMKEMGFASSPLCYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRIC 226
           DK+ +  +KM E GF  S L YN ++ +Y++ GQ +KVP ++ E+K     PD  +Y + 
Sbjct: 180 DKAEALFEKMGECGFLKSCLPYNHMLSMYISRGQFEKVPVLIKELKIR-TSPDIVTYNLW 239

Query: 227 INSYGARSDLISMEKV-LKEMESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDK 286
           + ++ + +D+   EKV LK  E +  ++ DW TYS++ N + K +  EKA   L++ E  
Sbjct: 240 LTAFASGNDVEGAEKVYLKAKEEK--LNPDWVTYSVLTNLYAKTDNVEKARLALKEMEKL 299

Query: 287 VD-QDALGLNHLISLYTSLGRKDEVRRLWALQKKCKKQVN-RDYITMLGSLVKLELLEEA 346
           V  ++ +    LISL+ +LG KD V   W   K   K++N  +Y++M+ ++VKL   E+A
Sbjct: 300 VSKKNRVAYASLISLHANLGDKDGVNLTWKKVKSSFKKMNDAEYLSMISAVVKLGEFEQA 359

Query: 347 ENLLKEWESSCQCYDFRVPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAG 406
           + L  EWES     D R+PN++L  Y  +  +   EK  + I+  G  P  ++W I+   
Sbjct: 360 KGLYDEWESVSGTGDARIPNLILAEYMNRDEVLLGEKFYERIVEKGINPSYSTWEILTWA 419

Query: 407 YLEKQELEKAFKCMKEAVAAQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTV 466
           YL+++++EK   C  +A+   +  K W     ++    + L E G  +  ++ +  L+  
Sbjct: 420 YLKRKDMEKVLDCFGKAI---DSVKKWTVNVRLVKGACKELEEQGNVKGAEKLMTLLQKA 479

Query: 467 PSMDGKLSDAFDKLLETLKNDDEVTTIGE 493
             ++ +L   ++ LL T     E+  I E
Sbjct: 480 GYVNTQL---YNSLLRTYAKAGEMALIVE 498

BLAST of Spg030252 vs. ExPASy TrEMBL
Match: A0A6J1JQ72 (pentatricopeptide repeat-containing protein At4g21705, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111486809 PE=4 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 2.0e-254
Identity = 441/488 (90.37%), Postives = 463/488 (94.88%), Query Frame = 0

Query: 1   MAAALAMFKILRGSSSGCTGTARTETDAFPFCFLALRLYSARRNCNRRNLFARISPLGAP 60
           +AAALAMFKILR  SSG T TARTETDA  FCF+ALRLYSARR CNRRNLFARISPLG+P
Sbjct: 45  LAAALAMFKILRSFSSGFTRTARTETDA--FCFVALRLYSARRTCNRRNLFARISPLGSP 104

Query: 61  ELSVVPVLDQWIEGEGRKIKDFELRRIVRDLRNCRRYGQALEVSEWMSSKGLFSLTTRDF 120
           ELSVVP+LDQWI+ EGR IKDFELRRIVRDLRNCRRYGQALEVSEWM SKGLFS TTRDF
Sbjct: 105 ELSVVPILDQWIQ-EGRMIKDFELRRIVRDLRNCRRYGQALEVSEWMRSKGLFSFTTRDF 164

Query: 121 AVQLDLIGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLVDKSLSHMQKMKEMG 180
           AVQLDLIGRV+GLDSAEKYF SVSNQ+E+GKLYGALLNCYVREGLVDK+LSHMQKMKEMG
Sbjct: 165 AVQLDLIGRVQGLDSAEKYFSSVSNQEEMGKLYGALLNCYVREGLVDKALSHMQKMKEMG 224

Query: 181 FASSPLCYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRICINSYGARSDLISME 240
           FASSPLCYNDIMCLY+NTGQVDKVPNVLSEMKENGVLPDN+SYRICI+SYGARSDLI M 
Sbjct: 225 FASSPLCYNDIMCLYLNTGQVDKVPNVLSEMKENGVLPDNYSYRICISSYGARSDLIGML 284

Query: 241 KVLKEMESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDKVDQDALGLNHLISLY 300
           KVLKEMESQTHISMDWTTYSMVANFFIKA MHEKAM+YLRKCEDKV+QDALG NHLISLY
Sbjct: 285 KVLKEMESQTHISMDWTTYSMVANFFIKAGMHEKAMSYLRKCEDKVNQDALGFNHLISLY 344

Query: 301 TSLGRKDEVRRLWALQKKCKKQVNRDYITMLGSLVKLELLEEAENLLKEWESSCQCYDFR 360
           TSLG KDEV RLWALQKKCKKQVNRDYITMLG LVKLE LEEAE L+KEW SSC+CYDFR
Sbjct: 345 TSLGCKDEVMRLWALQKKCKKQVNRDYITMLGCLVKLEFLEEAEKLVKEWVSSCECYDFR 404

Query: 361 VPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQELEKAFKCMKEA 420
           VPNVLLIGYS++GLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQ LEKAFKCMKEA
Sbjct: 405 VPNVLLIGYSKRGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQNLEKAFKCMKEA 464

Query: 421 VAAQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTVPSMDGKLSDAFDKLLET 480
           VA QEQNKGWRPKPSVLSSILRWLSENGRYEELKEFL+SLKTVPSMDGKLS+AFD+LLET
Sbjct: 465 VAVQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLSSLKTVPSMDGKLSNAFDELLET 524

Query: 481 LKNDDEVT 489
           LKN+DE T
Sbjct: 525 LKNNDETT 529

BLAST of Spg030252 vs. ExPASy TrEMBL
Match: A0A6J1FVG8 (pentatricopeptide repeat-containing protein At4g21705, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111447235 PE=4 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.3e-253
Identity = 437/488 (89.55%), Postives = 463/488 (94.88%), Query Frame = 0

Query: 1   MAAALAMFKILRGSSSGCTGTARTETDAFPFCFLALRLYSARRNCNRRNLFARISPLGAP 60
           +AAALAMFKILR  SSG T TARTETDA  FCF+ALRLYSARR CNRRNLFARISPLG+P
Sbjct: 49  LAAALAMFKILRSFSSGFTRTARTETDA--FCFVALRLYSARRTCNRRNLFARISPLGSP 108

Query: 61  ELSVVPVLDQWIEGEGRKIKDFELRRIVRDLRNCRRYGQALEVSEWMSSKGLFSLTTRDF 120
           ELSVVP+LDQWI+ EGR IKDFE+RRIVRDLRNCRRYGQAL+VSEWM SKGLFS TTRDF
Sbjct: 109 ELSVVPILDQWIQ-EGRMIKDFEMRRIVRDLRNCRRYGQALQVSEWMRSKGLFSFTTRDF 168

Query: 121 AVQLDLIGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLVDKSLSHMQKMKEMG 180
           AVQLDLIGRVRG+DSAEKYF SVSNQ+EIGKLYGALLNCYVREGLVDK+LSHMQKMKEMG
Sbjct: 169 AVQLDLIGRVRGIDSAEKYFSSVSNQEEIGKLYGALLNCYVREGLVDKALSHMQKMKEMG 228

Query: 181 FASSPLCYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRICINSYGARSDLISME 240
           FASSPLCYNDIMCLY+NTGQVDKVPNVLSEMK+NGVLPDN+SYRICI+SYGARSDLI M 
Sbjct: 229 FASSPLCYNDIMCLYLNTGQVDKVPNVLSEMKDNGVLPDNYSYRICISSYGARSDLIGML 288

Query: 241 KVLKEMESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDKVDQDALGLNHLISLY 300
           KVLKEMESQTHISMDWTTYSMVANFFIKA MHE+AM+YLRKCEDKV+QDALG NHLISLY
Sbjct: 289 KVLKEMESQTHISMDWTTYSMVANFFIKAGMHEQAMSYLRKCEDKVNQDALGFNHLISLY 348

Query: 301 TSLGRKDEVRRLWALQKKCKKQVNRDYITMLGSLVKLELLEEAENLLKEWESSCQCYDFR 360
           TSLGRKDEV RLWALQKKCKKQVNRDYITMLG LVKLE LEEAE L++EWESSC+CYDFR
Sbjct: 349 TSLGRKDEVMRLWALQKKCKKQVNRDYITMLGCLVKLEFLEEAEKLVEEWESSCECYDFR 408

Query: 361 VPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQELEKAFKCMKEA 420
           VPNVLLIGYSQ+GLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQ  E+AFKCMKEA
Sbjct: 409 VPNVLLIGYSQRGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQNPERAFKCMKEA 468

Query: 421 VAAQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTVPSMDGKLSDAFDKLLET 480
           VA QEQNKGWRPKPSVLSSILRWLSENGRYEELKEFL+SLK VPSMDGKLS+AFD+LLET
Sbjct: 469 VAVQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLSSLKAVPSMDGKLSNAFDELLET 528

Query: 481 LKNDDEVT 489
           LKN+DE T
Sbjct: 529 LKNNDETT 533

BLAST of Spg030252 vs. ExPASy TrEMBL
Match: A0A6J1CNQ2 (pentatricopeptide repeat-containing protein At4g21705, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111012782 PE=4 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 4.1e-244
Identity = 429/497 (86.32%), Postives = 457/497 (91.95%), Query Frame = 0

Query: 1   MAAALAMFKILRGSSSGCTGTARTETDAFPFCFLALRLYSARRNCNRRNLFARISPLGAP 60
           MAAALAMFKIL   SS    T RTETD+  FC L LRLYS RRNCNRRNLFARISPLG P
Sbjct: 1   MAAALAMFKIL-SRSSNFERTVRTETDS--FCSLVLRLYSTRRNCNRRNLFARISPLGDP 60

Query: 61  ELSVVPVLDQWIEGEGRKIKDFELRRIVRDLRNCRRYGQALEVSEWMSSKGLFSLTTRDF 120
           ELSVV +LDQWIE EGRKIKDFELRRIVRDLR+CRRYGQALEVSEWMSSKGLF LTTRDF
Sbjct: 61  ELSVVQILDQWIE-EGRKIKDFELRRIVRDLRSCRRYGQALEVSEWMSSKGLFPLTTRDF 120

Query: 121 AVQLDLIGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLVDKSLSHMQKMKEMG 180
           AVQLDLIGRVRGLDSAEKYF SVSNQ+EIGKLYGALLNCYVREGLVDKSL+HMQ+MK+MG
Sbjct: 121 AVQLDLIGRVRGLDSAEKYFSSVSNQEEIGKLYGALLNCYVREGLVDKSLTHMQEMKQMG 180

Query: 181 FASSPLCYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRICINSYGARSDLISME 240
           FAS+PL YNDIMCLY+NTG VDKVPNVLSEMKENGVLPDNFSYRICINSYGARSDLI+ME
Sbjct: 181 FASTPLNYNDIMCLYLNTGHVDKVPNVLSEMKENGVLPDNFSYRICINSYGARSDLITME 240

Query: 241 KVLKEMESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDKVDQDALGLNHLISLY 300
           KVLKEMESQ+HISMDWTTYSMVANFFIKA+MHE+A+NYLRKCEDKVD+DALG NHLISLY
Sbjct: 241 KVLKEMESQSHISMDWTTYSMVANFFIKASMHEEALNYLRKCEDKVDRDALGFNHLISLY 300

Query: 301 TSLGRKDEVRRLWALQK-KCKKQVNRDYITMLGSLVKLELLEEAENLLKEWESSCQCYDF 360
           TSLG  DEV RLWALQK KCKKQVNRDYITMLGSLVKLE LEEAENLLKEWESSCQCYDF
Sbjct: 301 TSLGHNDEVMRLWALQKEKCKKQVNRDYITMLGSLVKLERLEEAENLLKEWESSCQCYDF 360

Query: 361 RVPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQELEKAFKCMKE 420
           RVPNVLLIGYSQKGLIERAEKML++I  +GRIPPPNSW IIAAGYLEKQ LEKAFKCM E
Sbjct: 361 RVPNVLLIGYSQKGLIERAEKMLRSIAREGRIPPPNSWAIIAAGYLEKQNLEKAFKCMNE 420

Query: 421 AVAAQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTVPSMDGKLSDAFDKLLE 480
           A+A +EQN GWRPKPSV+SSILRWLSENGRY ELKEFL+SLKTVPSMDGKL +A D+L+E
Sbjct: 421 ALAVKEQNNGWRPKPSVVSSILRWLSENGRYGELKEFLSSLKTVPSMDGKLHNALDELVE 480

Query: 481 TLKNDDEVTTIGELEVR 497
           TL+ND E+   GEL+VR
Sbjct: 481 TLENDGEIAMTGELQVR 493

BLAST of Spg030252 vs. ExPASy TrEMBL
Match: A0A0A0L7Y2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G104890 PE=4 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 2.4e-236
Identity = 412/496 (83.06%), Postives = 451/496 (90.93%), Query Frame = 0

Query: 1   MAAALAMFKILRGSSSGCTGTARTETDAFPFCFLALRLYSARRNCNRRNLFARISPLGAP 60
           MAAA AMFKIL  SSSGCT T R ETDA  FCF+ALRLYS RR+C+RRNL+ARISPLG P
Sbjct: 1   MAAASAMFKILSRSSSGCTRTLRPETDA--FCFVALRLYSTRRSCDRRNLYARISPLGDP 60

Query: 61  ELSVVPVLDQWIEGEGRKIKDFELRRIVRDLRNCRRYGQALEVSEWMSSKGLFSLTTRDF 120
           E +VVPVL+QWIE EGR IKDFELRRIVRDLR CRRY QALEVSEWM SKGLFSLTTRDF
Sbjct: 61  ECTVVPVLNQWIE-EGRNIKDFELRRIVRDLRTCRRYRQALEVSEWMCSKGLFSLTTRDF 120

Query: 121 AVQLDLIGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLVDKSLSHMQKMKEMG 180
           A+QLDLIG+VRGLDSAEKYFGSVSNQ+EIGKLYGALLNCYVREGL+DKSL+HMQKMKEMG
Sbjct: 121 AIQLDLIGQVRGLDSAEKYFGSVSNQKEIGKLYGALLNCYVREGLIDKSLAHMQKMKEMG 180

Query: 181 FASSPLCYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRICINSYGARSDLISME 240
            ASSPLCYNDIMCLY+NTGQ DKVPNVLSEMKENGVLPDNFSYRICI+SYGARSD+ISME
Sbjct: 181 LASSPLCYNDIMCLYLNTGQADKVPNVLSEMKENGVLPDNFSYRICISSYGARSDVISME 240

Query: 241 KVLKEMESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDKVDQDALGLNHLISLY 300
            VLKEME QTHISMDWTTYSMVA FFIKA MH+KAMNYLRKCEDKVD+DALG NHLIS Y
Sbjct: 241 NVLKEMEGQTHISMDWTTYSMVAGFFIKAGMHDKAMNYLRKCEDKVDEDALGFNHLISHY 300

Query: 301 TSLGRKDEVRRLWALQKKCKKQVNRDYITMLGSLVKLELLEEAENLLKEWESSCQCYDFR 360
           T+LG K+EV RLWAL KK KKQ+NRDYITMLGSLVKLELLEEAENL+ EWESSCQCYDFR
Sbjct: 301 TNLGHKNEVMRLWALLKKGKKQLNRDYITMLGSLVKLELLEEAENLVMEWESSCQCYDFR 360

Query: 361 VPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQELEKAFKCMKEA 420
           VPNV+LIGYSQKGLIE+AEKML+NII +G IP PNSWGIIA+GYLEKQ LEKAF+CMKEA
Sbjct: 361 VPNVVLIGYSQKGLIEKAEKMLRNIIVNGMIPSPNSWGIIASGYLEKQNLEKAFECMKEA 420

Query: 421 VAAQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTVPSMDGKLSDAFDKLLET 480
           +A + QNK WRPKP+VLSSILRWLSEN RYEE+KEF++SLKTVPSMD KL++A D+LLE 
Sbjct: 421 LAVKGQNKVWRPKPNVLSSILRWLSENRRYEEMKEFMSSLKTVPSMDEKLNNALDELLEI 480

Query: 481 LKNDDEVTTIGELEVR 497
           + NDD ++   ELEV+
Sbjct: 481 MANDDGISK-DELEVK 492

BLAST of Spg030252 vs. ExPASy TrEMBL
Match: A0A1S4DZ16 (pentatricopeptide repeat-containing protein At4g21705, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC103493335 PE=4 SV=1)

HSP 1 Score: 810.4 bits (2092), Expect = 4.0e-231
Identity = 401/485 (82.68%), Postives = 439/485 (90.52%), Query Frame = 0

Query: 1   MAAALAMFKILRGSSSGCTGTARTETDAFPFCFLALRLYSARRNCNRRNLFARISPLGAP 60
           MAAA AMFKIL  SSSGCT T R ETDA  FCF+ALRLYS RR+CNRR L+A ISPLG P
Sbjct: 1   MAAASAMFKILSRSSSGCTRTPRPETDA--FCFVALRLYSTRRSCNRRKLYAMISPLGDP 60

Query: 61  ELSVVPVLDQWIEGEGRKIKDFELRRIVRDLRNCRRYGQALEVSEWMSSKGLFSLTTRDF 120
           + SVVPVL+QWI+ EGRKIKDFELRRIVRDLR CRRY QALEVSEWM SKG FSLTTRDF
Sbjct: 61  DSSVVPVLNQWIK-EGRKIKDFELRRIVRDLRTCRRYRQALEVSEWMCSKGRFSLTTRDF 120

Query: 121 AVQLDLIGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLVDKSLSHMQKMKEMG 180
           A+QLDLIG+VRGLDSAEKYFGSVS Q+EIGKLYG+LLNCYVREGL+DKSL+HMQKMKEMG
Sbjct: 121 AIQLDLIGQVRGLDSAEKYFGSVSKQKEIGKLYGSLLNCYVREGLIDKSLAHMQKMKEMG 180

Query: 181 FASSPLCYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRICINSYGARSDLISME 240
           FASSPLCYNDIMCLY+NTGQ DKVPNVLSEMKENGVLPDNFSYRICI+SYGARSD+ISME
Sbjct: 181 FASSPLCYNDIMCLYLNTGQADKVPNVLSEMKENGVLPDNFSYRICISSYGARSDVISME 240

Query: 241 KVLKEMESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDKVDQDALGLNHLISLY 300
            VLKEMESQTHISMDW TYSMVA FFIK  MH+KA NYLRKCED+VDQDALG NHLIS Y
Sbjct: 241 NVLKEMESQTHISMDWITYSMVAGFFIKVVMHDKARNYLRKCEDRVDQDALGFNHLISHY 300

Query: 301 TSLGRKDEVRRLWALQKKCKKQVNRDYITMLGSLVKLELLEEAENLLKEWESSCQCYDFR 360
           T+LG K+EV RLWALQKK KKQ+NRDYITMLGSLVKL+LLEEAENL+ EWESSCQC DFR
Sbjct: 301 TNLGHKNEVMRLWALQKKAKKQLNRDYITMLGSLVKLDLLEEAENLVMEWESSCQCNDFR 360

Query: 361 VPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQELEKAFKCMKEA 420
           VPNV+LIGYSQ GLIE+AEKML+NII +G IP PNSWGIIA+GYLEKQ LEKAF+CMKEA
Sbjct: 361 VPNVVLIGYSQNGLIEKAEKMLRNIIVNGMIPSPNSWGIIASGYLEKQNLEKAFECMKEA 420

Query: 421 VAAQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTVPSMDGKLSDAFDKLLET 480
           +A + QNK WRPKP+VLSSILRWLSEN RYEE+KEF++SLKTVPSMD KL+ A D+LLE 
Sbjct: 421 LAVKGQNKVWRPKPNVLSSILRWLSENRRYEEMKEFMSSLKTVPSMDEKLNSALDELLEI 480

Query: 481 LKNDD 486
           ++NDD
Sbjct: 481 MENDD 482

BLAST of Spg030252 vs. TAIR 10
Match: AT4G21705.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 419.9 bits (1078), Expect = 2.9e-117
Identity = 217/479 (45.30%), Postives = 312/479 (65.14%), Query Frame = 0

Query: 30  PFCFLALRLYSARRNCNRRNLFARISPLGAPELSVVPVLDQWIEGEGRKIKDFELRRIVR 89
           P   +A R Y   R   +  L+++ISPLG P+ SV P L  W++  G+K+   EL RIV 
Sbjct: 8   PANLIASRYYYTNR-VKKTTLYSKISPLGDPKSSVYPELQNWVQC-GKKVSVAELIRIVH 67

Query: 90  DLRNCRRYGQALEVSEWMSSKGLFSLTTRDFAVQLDLIGRVRGLDSAEKYFGSVSNQQEI 149
           DLR  +R+  ALEVS+WM+  G+   +  + AV LDLIGRV G  +AE+YF ++  Q + 
Sbjct: 68  DLRRRKRFLHALEVSKWMNETGVCVFSPTEHAVHLDLIGRVYGFVTAEEYFENLKEQYKN 127

Query: 150 GKLYGALLNCYVREGLVDKSLSHMQKMKEMGFASSPLCYNDIMCLYMNTGQVDKVPNVLS 209
            K YGALLNCYVR+  V+KSL H +KMKEMGF +S L YN+IMCLY N GQ +KVP VL 
Sbjct: 128 DKTYGALLNCYVRQQNVEKSLLHFEKMKEMGFVTSSLTYNNIMCLYTNIGQHEKVPKVLE 187

Query: 210 EMKENGVLPDNFSYRICINSYGARSDLISMEKVLKEMESQTHISMDWTTYSMVANFFIKA 269
           EMKE  V PDN+SYRICIN++GA  DL  +   L++ME +  I+MDW TY++ A F+I  
Sbjct: 188 EMKEENVAPDNYSYRICINAFGAMYDLERIGGTLRDMERRQDITMDWNTYAVAAKFYIDG 247

Query: 270 NMHEKAMNYLRKCEDKVD-QDALGLNHLISLYTSLGRKDEVRRLWALQKK-CKKQVNRDY 329
              ++A+  L+  E++++ +D  G NHLI+LY  LG+K EV RLW L+K  CK+++N+DY
Sbjct: 248 GDCDRAVELLKMSENRLEKKDGEGYNHLITLYARLGKKIEVLRLWDLEKDVCKRRINQDY 307

Query: 330 ITMLGSLVKLELLEEAENLLKEWESSCQCYDFRVPNVLLIGYSQKGLIERAEKMLQNIIS 389
           +T+L SLVK++ L EAE +L EW+SS  CYDFRVPN ++ GY  K + E+AE ML+++  
Sbjct: 308 LTVLQSLVKIDALVEAEEVLTEWKSSGNCYDFRVPNTVIRGYIGKSMEEKAEAMLEDLAR 367

Query: 390 DGRIPPPNSWGIIAAGYLEKQELEKAFKCMKEAVAAQEQNKGWRPKPSVLSSILRWLSEN 449
            G+   P SW ++A  Y EK  LE AFKCMK A+  +  ++ WRP  ++++S+L W+ + 
Sbjct: 368 RGKATTPESWELVATAYAEKGTLENAFKCMKTALGVEVGSRKWRPGLTLVTSVLSWVGDE 427

Query: 450 GRYEELKEFLNSLKTVPSMDGKLSDA------------FDKLLETLKND----DEVTTI 491
           G  +E++ F+ SL+    ++ ++  A             D LL+ +K+D    DE TT+
Sbjct: 428 GSLKEVESFVASLRNCIGVNKQMYHALVKADIREGGRNIDTLLQRMKDDKIEIDEETTV 484

BLAST of Spg030252 vs. TAIR 10
Match: AT2G20710.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 285.8 bits (730), Expect = 6.6e-77
Identity = 153/428 (35.75%), Postives = 252/428 (58.88%), Query Frame = 0

Query: 53  RISPLGAPELSVVPVLDQWIEGEGRKIKDFELRRIVRDLRNCRRYGQALEVSEWMSSKGL 112
           R++  G P  S++ VLD W++ +G  +K  EL  I++ LR   R+  AL++S+WMS   +
Sbjct: 43  RVARSGDPSASIIKVLDGWLD-QGNLVKTSELHSIIKMLRKFSRFSHALQISDWMSEHRV 102

Query: 113 FSLTTRDFAVQLDLIGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLVDKSLSH 172
             ++  D A++LDLI +V GL  AEK+F ++  ++    LYGALLNCY  + ++ K+   
Sbjct: 103 HEISEGDVAIRLDLIAKVGGLGEAEKFFETIPMERRNYHLYGALLNCYASKKVLHKAEQV 162

Query: 173 MQKMKEMGFASSPLCYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRICINSYGA 232
            Q+MKE+GF    L YN ++ LY+ TG+   V  +L EM++  V PD F+    +++Y  
Sbjct: 163 FQEMKELGFLKGCLPYNVMLNLYVRTGKYTMVEKLLREMEDETVKPDIFTVNTRLHAYSV 222

Query: 233 RSDLISMEKVLKEMESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDKVDQDAL- 292
            SD+  MEK L   E+   + +DW TY+  AN +IKA + EKA+  LRK E  V+     
Sbjct: 223 VSDVEGMEKFLMRCEADQGLHLDWRTYADTANGYIKAGLTEKALEMLRKSEQMVNAQKRK 282

Query: 293 -GLNHLISLYTSLGRKDEVRRLWALQKKCKKQVNRDYITMLGSLVKLELLEEAENLLKEW 352
                L+S Y + G+K+EV RLW+L K+     N  YI+++ +L+K++ +EE E +++EW
Sbjct: 283 HAYEVLMSFYGAAGKKEEVYRLWSLYKELDGFYNTGYISVISALLKMDDIEEVEKIMEEW 342

Query: 353 ESSCQCYDFRVPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLEKQEL 412
           E+    +D R+P++L+ GY +KG++E+AE+++  ++   R+   ++W  +A GY    ++
Sbjct: 343 EAGHSLFDIRIPHLLITGYCKKGMMEKAEEVVNILVQKWRVEDTSTWERLALGYKMAGKM 402

Query: 413 EKAFKCMKEAVAAQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTVPSMDGKL 472
           EKA +  K A+  +    GWRP   VL S + +L      E L++ L  L    S  G +
Sbjct: 403 EKAVEKWKRAI--EVSKPGWRPHQVVLMSCVDYLEGQRDMEGLRKILRLL----SERGHI 461

Query: 473 SDAFDKLL 479
           S  +D+LL
Sbjct: 463 S--YDQLL 461

BLAST of Spg030252 vs. TAIR 10
Match: AT1G02150.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 273.1 bits (697), Expect = 4.4e-73
Identity = 157/449 (34.97%), Postives = 257/449 (57.24%), Query Frame = 0

Query: 50  LFARISPLGAPELSVVPVLDQWIEGEGRKIKDFELRRIVRDLRNCRRYGQALEVSEWMSS 109
           ++ +IS +  PEL    VL+QW E  GRK+  +EL R+V++LR  +R  QALEV +WM++
Sbjct: 69  IYKKISLMEKPELGAASVLNQW-EKAGRKLTKWELCRVVKELRKYKRANQALEVYDWMNN 128

Query: 110 KG-LFSLTTRDFAVQLDLIGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLVDK 169
           +G  F L+  D A+QLDLIG+VRG+  AE++F  +    +  ++YG+LLN YVR    +K
Sbjct: 129 RGERFRLSASDAAIQLDLIGKVRGIPDAEEFFLQLPENFKDRRVYGSLLNAYVRAKSREK 188

Query: 170 SLSHMQKMKEMGFASSPLCYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRICIN 229
           + + +  M++ G+A  PL +N +M LYMN  + DKV  ++ EMK+  +  D +SY I ++
Sbjct: 189 AEALLNTMRDKGYALHPLPFNVMMTLYMNLREYDKVDAMVFEMKQKDIRLDIYSYNIWLS 248

Query: 230 SYGARSDLISMEKVLKEMESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDKV-D 289
           S G+   +  ME V ++M+S   I  +WTT+S +A  +IK    EKA + LRK E ++  
Sbjct: 249 SCGSLGSVEKMELVYQQMKSDVSIYPNWTTFSTMATMYIKMGETEKAEDALRKVEARITG 308

Query: 290 QDALGLNHLISLYTSLGRKDEVRRLWALQKKCKKQV-NRDYITMLGSLVKLELLEEAENL 349
           ++ +  ++L+SLY SLG K E+ R+W + K     + N  Y  ++ SLV++  +E AE +
Sbjct: 309 RNRIPYHYLLSLYGSLGNKKELYRVWHVYKSVVPSIPNLGYHALVSSLVRMGDIEGAEKV 368

Query: 350 LKEWESSCQCYDFRVPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGYLE 409
            +EW      YD R+PN+L+  Y +   +E AE +  +++  G  P  ++W I+A G+  
Sbjct: 369 YEEWLPVKSSYDPRIPNLLMNAYVKNDQLETAEGLFDHMVEMGGKPSSSTWEILAVGHTR 428

Query: 410 KQELEKAFKCMKEAVAAQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTVPSM 469
           K+ + +A  C++ A +A E +  WRPK  +LS   +   E       +  L  L+     
Sbjct: 429 KRCISEALTCLRNAFSA-EGSSNWRPKVLMLSGFFKLCEEESDVTSKEAVLELLR----- 488

Query: 470 DGKLSDAFDKLLETLKNDDEVTTIGELEV 496
             +  D  DK    L + DE  T+   E+
Sbjct: 489 --QSGDLEDKSYLALIDVDENRTVNNSEI 508

BLAST of Spg030252 vs. TAIR 10
Match: AT1G60770.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 2.5e-68
Identity = 145/458 (31.66%), Postives = 251/458 (54.80%), Query Frame = 0

Query: 34  LALRLYSARRNCNRRN--------LFARISPLGAPELSVVPVLDQWIEGEGRKIKDFELR 93
           +A+R  S  R+  +R+        L+ R+   G  E+ V   L+Q+++G     K +E+ 
Sbjct: 1   MAMRHLSRSRDVTKRSTKKYIEEPLYNRLFKDGGTEVKVRQQLNQFLKGTKHVFK-WEVG 60

Query: 94  RIVRDLRNCRRYGQALEVSEWMSSKGLFSLTTRDFAVQLDLIGRVRGLDSAEKYFGSVSN 153
             ++ LRN   Y  AL++SE M  +G+ + T  D A+ LDL+ + R + + E YF  +  
Sbjct: 61  DTIKKLRNRGLYYPALKLSEVMEERGM-NKTVSDQAIHLDLVAKAREITAGENYFVDLPE 120

Query: 154 QQEIGKLYGALLNCYVREGLVDKSLSHMQKMKEMGFASSPLCYNDIMCLYMNTGQVDKVP 213
             +    YG+LLNCY +E L +K+   + KMKE+    S + YN +M LY  TG+ +KVP
Sbjct: 121 TSKTELTYGSLLNCYCKELLTEKAEGLLNKMKELNITPSSMSYNSLMTLYTKTGETEKVP 180

Query: 214 NVLSEMKENGVLPDNFSYRICINSYGARSDLISMEKVLKEMESQTHISMDWTTYSMVANF 273
            ++ E+K   V+PD+++Y + + +  A +D+  +E+V++EM     ++ DWTTYS +A+ 
Sbjct: 181 AMIQELKAENVMPDSYTYNVWMRALAATNDISGVERVIEEMNRDGRVAPDWTTYSNMASI 240

Query: 274 FIKANMHEKAMNYLRKCEDK-VDQDALGLNHLISLYTSLGRKDEVRRLW-ALQKKCKKQV 333
           ++ A + +KA   L++ E K   +D      LI+LY  LG+  EV R+W +L+    K  
Sbjct: 241 YVDAGLSQKAEKALQELEMKNTQRDFTAYQFLITLYGRLGKLTEVYRIWRSLRLAIPKTS 300

Query: 334 NRDYITMLGSLVKLELLEEAENLLKEWESSCQCYDFRVPNVLLIGYSQKGLIERAEKMLQ 393
           N  Y+ M+  LVKL  L  AE L KEW+++C  YD R+ NVL+  Y+Q+GLI++A ++ +
Sbjct: 301 NVAYLNMIQVLVKLNDLPGAETLFKEWQANCSTYDIRIVNVLIGAYAQEGLIQKANELKE 360

Query: 394 NIISDGRIPPPNSWGIIAAGYLEKQELEKAFKCMKEAVAAQEQNKG-WRPKPSVLSSILR 453
                G      +W I    Y++  ++ +A +CM +AV+  + + G W P P  + +++ 
Sbjct: 361 KAPRRGGKLNAKTWEIFMDYYVKSGDMARALECMSKAVSIGKGDGGKWLPSPETVRALMS 420

Query: 454 WLSENGRYEELKEFLNSLKTVPSMDGKLSDAFDKLLET 481
           +  +       +  L  LK     D   ++ F+ L+ T
Sbjct: 421 YFEQKKDVNGAENLLEILKN--GTDNIGAEIFEPLIRT 454

BLAST of Spg030252 vs. TAIR 10
Match: AT2G20710.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 242.3 bits (617), Expect = 8.4e-64
Identity = 133/374 (35.56%), Postives = 218/374 (58.29%), Query Frame = 0

Query: 107 MSSKGLFSLTTRDFAVQLDLIGRVRGLDSAEKYFGSVSNQQEIGKLYGALLNCYVREGLV 166
           MS   +  ++  D A++LDLI +V GL  AEK+F ++  ++    LYGALLNCY  + ++
Sbjct: 1   MSEHRVHEISEGDVAIRLDLIAKVGGLGEAEKFFETIPMERRNYHLYGALLNCYASKKVL 60

Query: 167 DKSLSHMQKMKEMGFASSPLCYNDIMCLYMNTGQVDKVPNVLSEMKENGVLPDNFSYRIC 226
            K+    Q+MKE+GF    L YN ++ LY+ TG+   V  +L EM++  V PD F+    
Sbjct: 61  HKAEQVFQEMKELGFLKGCLPYNVMLNLYVRTGKYTMVEKLLREMEDETVKPDIFTVNTR 120

Query: 227 INSYGARSDLISMEKVLKEMESQTHISMDWTTYSMVANFFIKANMHEKAMNYLRKCEDKV 286
           +++Y   SD+  MEK L   E+   + +DW TY+  AN +IKA + EKA+  LRK E  V
Sbjct: 121 LHAYSVVSDVEGMEKFLMRCEADQGLHLDWRTYADTANGYIKAGLTEKALEMLRKSEQMV 180

Query: 287 DQDAL--GLNHLISLYTSLGRKDEVRRLWALQKKCKKQVNRDYITMLGSLVKLELLEEAE 346
           +          L+S Y + G+K+EV RLW+L K+     N  YI+++ +L+K++ +EE E
Sbjct: 181 NAQKRKHAYEVLMSFYGAAGKKEEVYRLWSLYKELDGFYNTGYISVISALLKMDDIEEVE 240

Query: 347 NLLKEWESSCQCYDFRVPNVLLIGYSQKGLIERAEKMLQNIISDGRIPPPNSWGIIAAGY 406
            +++EWE+    +D R+P++L+ GY +KG++E+AE+++  ++   R+   ++W  +A GY
Sbjct: 241 KIMEEWEAGHSLFDIRIPHLLITGYCKKGMMEKAEEVVNILVQKWRVEDTSTWERLALGY 300

Query: 407 LEKQELEKAFKCMKEAVAAQEQNKGWRPKPSVLSSILRWLSENGRYEELKEFLNSLKTVP 466
               ++EKA +  K A+  +    GWRP   VL S + +L      E L++ L  L    
Sbjct: 301 KMAGKMEKAVEKWKRAI--EVSKPGWRPHQVVLMSCVDYLEGQRDMEGLRKILRLL---- 360

Query: 467 SMDGKLSDAFDKLL 479
           S  G +S  +D+LL
Sbjct: 361 SERGHIS--YDQLL 366

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6600732.12.2e-25590.57Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
XP_023545982.11.8e-25490.16pentatricopeptide repeat-containing protein At4g21705, mitochondrial-like [Cucur... [more]
XP_022989754.14.1e-25490.37pentatricopeptide repeat-containing protein At4g21705, mitochondrial-like [Cucur... [more]
XP_022942045.12.6e-25389.55pentatricopeptide repeat-containing protein At4g21705, mitochondrial-like [Cucur... [more]
KAG7031372.13.5e-25390.46Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
Match NameE-valueIdentityDescription
Q84JR34.1e-11645.30Pentatricopeptide repeat-containing protein At4g21705, mitochondrial OS=Arabidop... [more]
Q9SKU69.3e-7635.75Pentatricopeptide repeat-containing protein At2g20710, mitochondrial OS=Arabidop... [more]
Q8LPS66.2e-7234.97Pentatricopeptide repeat-containing protein At1g02150 OS=Arabidopsis thaliana OX... [more]
O227143.5e-6731.66Pentatricopeptide repeat-containing protein At1g60770 OS=Arabidopsis thaliana OX... [more]
Q9SY074.5e-6232.74Pentatricopeptide repeat-containing protein At4g02820, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1JQ722.0e-25490.37pentatricopeptide repeat-containing protein At4g21705, mitochondrial-like OS=Cuc... [more]
A0A6J1FVG81.3e-25389.55pentatricopeptide repeat-containing protein At4g21705, mitochondrial-like OS=Cuc... [more]
A0A6J1CNQ24.1e-24486.32pentatricopeptide repeat-containing protein At4g21705, mitochondrial-like OS=Mom... [more]
A0A0A0L7Y22.4e-23683.06Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G104890 PE=4 SV=1[more]
A0A1S4DZ164.0e-23182.68pentatricopeptide repeat-containing protein At4g21705, mitochondrial-like OS=Cuc... [more]
Match NameE-valueIdentityDescription
AT4G21705.12.9e-11745.30Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G20710.16.6e-7735.75Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G02150.14.4e-7334.97Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G60770.12.5e-6831.66Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G20710.28.4e-6435.56Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 407..427
NoneNo IPR availablePANTHERPTHR45717OS12G0527900 PROTEINcoord: 37..477
NoneNo IPR availablePANTHERPTHR45717:SF7PPR CONTAINING PLANT-LIKE PROTEINcoord: 37..477
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 313..463
e-value: 5.0E-14
score: 54.0
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 81..270
e-value: 3.0E-23
score: 84.7
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 160..429
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 153..181
e-value: 2.1E-4
score: 21.3
coord: 363..389
e-value: 0.035
score: 14.3
coord: 327..352
e-value: 0.18
score: 12.2
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 152..181
e-value: 8.4E-4
score: 17.4
coord: 187..219
e-value: 2.0E-5
score: 22.5
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 187..230
e-value: 5.7E-9
score: 36.1
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 184..218
score: 9.865228
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 149..183
score: 8.560833

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg030252.1Spg030252.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005515 protein binding