Spg030209 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg030209 vs. NCBI nr
Match: XP_022929340.1 (fibroin heavy chain-like [Cucurbita moschata])

HSP 1 Score: 190.3 bits (482), Expect = 2.0e-44
Identity = 130/257 (50.58%), Postives = 154/257 (59.92%), Query Frame = 0

Query: 1   MSSLKYLLLCPFVFLCLSSTFANKLLNSNYGHGSGPDIGFG----PRAGPGVEGDAINIG 60
           M SLKY LL PFVFLCLS TFAN++ NS+   GSG D+G G    P AGPGVE    N+ 
Sbjct: 1   MGSLKYFLLSPFVFLCLSCTFANRVPNSD--DGSGFDVGAGPGAIPTAGPGVEKGVSNVR 60

Query: 61  AGPKAGPWAGPGGKGVSNFGAGPKAGPGGVSHVG-------GGPRAGPGIEGRVSSVGAG 120
           AGP A  W      G+    AGPKAGPG    V         GP+AGPG EG VS+V AG
Sbjct: 61  AGPAAEGWVNDVWAGLK---AGPKAGPGAEGWVSDVKAGLRAGPKAGPGAEGWVSNVKAG 120

Query: 121 PEIERGVSVGAGPRAGP-----------GARRGVDPIVNGVGVGIGV--GYRPGF----- 180
           P +        GPRA P           G RR VDP++NG+G+G+GV  GYR GF     
Sbjct: 121 PTV--------GPRAWPGTEGGVSSSEGGVRRDVDPMINGLGLGLGVDIGYRSGFRAGLG 180

Query: 181 -GPGWNGPGI---GGGLYGDCILGYVCPANERRECNKFAYESCESFNFHPLTASMDLHEV 224
            G  W GPGI   GGG+  +C LGYVCP   RR C+KF+Y +C+++ FHPL ASM LHEV
Sbjct: 181 GGEHWFGPGIGIGGGGVSNECTLGYVCPTYGRRGCDKFSYGNCDTYGFHPLMASMHLHEV 240

BLAST of Spg030209 vs. NCBI nr
Match: KAG6577377.1 (hypothetical protein SDJN03_24951, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 187.2 bits (474), Expect = 1.7e-43
Identity = 145/292 (49.66%), Postives = 167/292 (57.19%), Query Frame = 0

Query: 1   MSSLKYLLLCPFVFLCLSSTFANKLLNSNYGHGSGPDIGFG----PRAGPGVEGDAINIG 60
           M SLKY LL PFVFLCLS TFAN++ NS+   GSG D+G G    P AGPGVE    N+ 
Sbjct: 1   MGSLKYFLLSPFVFLCLSCTFANRVPNSD--DGSGFDVGAGPGAIPTAGPGVEKGVSNVR 60

Query: 61  AGP-------------KAGPWAGPGGKG-VSNF--------GAGPKAGPGG---VSHVGG 120
           AGP             KAGP AGPG +G VS+          AGPKAGPG    VS V  
Sbjct: 61  AGPAAEGWVNDVRAGLKAGPKAGPGAEGWVSDVKAGLRAGPKAGPKAGPGAEEWVSDVKA 120

Query: 121 GPR----AGPGIEGRVSSV--------GAGPEIERGVS-VGAGPRAGP------------ 180
           GPR    AGPG EG VS V         AGP  E  VS V AGP  GP            
Sbjct: 121 GPRAGPKAGPGAEGWVSDVKAGLRAGPKAGPGAEGWVSNVKAGPTVGPRAWPGTEGGVSS 180

Query: 181 ---GARRGVDPIVNGVGVGIGV--GYRPGF------GPGWNGPGI---GGGLYGDCILGY 224
              G RR VDP++NG+G+G+GV  GYR GF      G  W GPGI   GGG+  +C LGY
Sbjct: 181 SEGGVRRDVDPMINGLGLGLGVDIGYRSGFRAGLGGGEHWFGPGIGIGGGGVSNECTLGY 240

BLAST of Spg030209 vs. NCBI nr
Match: KGN56231.1 (hypothetical protein Csa_011503 [Cucumis sativus])

HSP 1 Score: 186.0 bits (471), Expect = 3.7e-43
Identity = 126/249 (50.60%), Postives = 155/249 (62.25%), Query Frame = 0

Query: 1   MSSLKYLLLCPFVFLCLSSTFANKLLNSNYGHGSGPDIGFGPRAGPGVEGDAINIGAGPK 60
           M+SLKY LL PF+FLCLS TFAN + N + G      +GFG  + P  +           
Sbjct: 1   MASLKYFLLSPFLFLCLSYTFANGVFNYDDG------LGFGSMSSPTPD----------- 60

Query: 61  AGPWAGPGGKGVSNFGAGPKAGP------GGVSHVGG----GPRAGPGIEGRVSSVGAGP 120
             P AGP  +GVSNFG GPKAGP      GG+S+V      GP+AGPG++  +S+VGAGP
Sbjct: 61  --PSAGPVDRGVSNFGIGPKAGPRAGLGVGGISNVDDGSDPGPKAGPGVKEEMSNVGAGP 120

Query: 121 EIER-GV-SVGAGPRAGPGARRGVDPIVNGVGVGIGV-----------GYRPGFGPGWNG 180
            + + GV S+ AGPRAGP   +GVDPIV G+GVG+GV           G RPG G GW G
Sbjct: 121 RVPKLGVSSIEAGPRAGP---KGVDPIVTGLGVGVGVNLPPIFGGPKMGIRPGPG-GWYG 180

Query: 181 PG-IGGGLYGDCILGYVCPANERRECNKFAYESCESFNFHPLTASMDLHEVEINWAKSKP 226
           PG I    Y +C+LGYVCP N    C K  Y  CES+NF PL+AS +LH+V+INWAKSK 
Sbjct: 181 PGPIIQEPYNNCMLGYVCPTNRPWACGKVGYGLCESYNFRPLSASTELHDVKINWAKSKS 226

BLAST of Spg030209 vs. NCBI nr
Match: XP_023551823.1 (fibroin heavy chain-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 160.6 bits (405), Expect = 1.7e-35
Identity = 110/216 (50.93%), Postives = 134/216 (62.04%), Query Frame = 0

Query: 41  GPRAGPGVEGDAINIGAGPKAGPWAGPGG-KGVSNFGAGPKAGP-------GGVSHVGGG 100
           GP+AGPG EG   ++ AGP+AG  AGPG  + VS+  AGP+AGP       G VS V  G
Sbjct: 46  GPKAGPGAEGWVSDVKAGPRAGLKAGPGAEEWVSDVKAGPRAGPKAGPGAEGWVSDVKAG 105

Query: 101 PR----AGPGIEGRVSSVGAGPEIERGVSVGAGPRAGP-----------GARRGVDPIVN 160
           PR    AGPG EG V++V AGP +        GPRA P           G RR VDP++N
Sbjct: 106 PRAGPKAGPGAEGWVNNVKAGPTV--------GPRAWPGTEGGVSSSEGGVRRDVDPMIN 165

Query: 161 GVGVGIGV--GYRPGF------GPGWNGPGIGG-GLYGDCILGYVCPANERRECNKFAYE 220
           G+G+G+GV  GYR GF      G  W GPGIGG G+  +C LGYVCP   RR C+KF+Y 
Sbjct: 166 GLGLGLGVDIGYRSGFRAGVGGGEHWFGPGIGGRGVSNECTLGYVCPTYGRRGCDKFSYG 225

Query: 221 SCESFNFHPLTASMDLHEVEINWAK-SKPVAMAQHG 224
           +C+S+ FHPL ASM LHEVE+ WAK SKP A  Q+G
Sbjct: 226 NCDSYGFHPLMASMQLHEVEMKWAKGSKPAATPQNG 253

BLAST of Spg030209 vs. NCBI nr
Match: KAA0060661.1 (hypothetical protein E6C27_scaffold22G005540 [Cucumis melo var. makuwa] >TYK02214.1 hypothetical protein E5676_scaffold18G00010 [Cucumis melo var. makuwa])

HSP 1 Score: 157.5 bits (397), Expect = 1.4e-34
Identity = 119/240 (49.58%), Postives = 141/240 (58.75%), Query Frame = 0

Query: 1   MSSLKYLLLCPFVFLCLSSTFANKLLNSNYG--HGSGPDIGFGPRAGPGVEGDAINIGAG 60
           M+SLKY LL PF+FLCLS TFA+ + N ++G   GS       P AGPGV+    NIG G
Sbjct: 1   MASLKYFLLSPFLFLCLSYTFADGVFNYDHGLDFGSMSSPTPDPSAGPGVDIGVSNIGIG 60

Query: 61  PKAGPWAGPG-GKGVSNFGAGPKAGPGGVSHVGGGPRAGPGIEGRVSSVGAGPEIERGVS 120
           PKAGP AG G G G+S+    P            GP+AGP   G       G ++  GVS
Sbjct: 61  PKAGPRAGLGIGGGISDVDDEP------------GPKAGPKASG-------GHKL--GVS 120

Query: 121 -VGAGPRAGPGARRGVDPIVNGVGVGIGVGYRPGFG----------PGWNGPG-IGGGLY 180
            + AGPRAGP         VNG GVG+GV   P FG           GW  PG I    Y
Sbjct: 121 GIEAGPRAGPKG-------VNGFGVGVGVDLPPVFGGPKIGLKPGPGGWYRPGPIIQEPY 180

Query: 181 GDCILGYVCPANERRECNKFAYESCESFNFHPLTASMDLHEVEINWAKSKPVAMAQHGKS 226
           G+C+LGYVCP N    C+KFAY  C+S+NFHPL+AS DLHEV+INWAKSKP A AQHG+S
Sbjct: 181 GNCMLGYVCP-NRPWACSKFAYGLCDSYNFHPLSASTDLHEVKINWAKSKPDATAQHGES 211

BLAST of Spg030209 vs. ExPASy TrEMBL
Match: A0A6J1EU53 (fibroin heavy chain-like OS=Cucurbita moschata OX=3662 GN=LOC111435943 PE=4 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 9.5e-45
Identity = 130/257 (50.58%), Postives = 154/257 (59.92%), Query Frame = 0

Query: 1   MSSLKYLLLCPFVFLCLSSTFANKLLNSNYGHGSGPDIGFG----PRAGPGVEGDAINIG 60
           M SLKY LL PFVFLCLS TFAN++ NS+   GSG D+G G    P AGPGVE    N+ 
Sbjct: 1   MGSLKYFLLSPFVFLCLSCTFANRVPNSD--DGSGFDVGAGPGAIPTAGPGVEKGVSNVR 60

Query: 61  AGPKAGPWAGPGGKGVSNFGAGPKAGPGGVSHVG-------GGPRAGPGIEGRVSSVGAG 120
           AGP A  W      G+    AGPKAGPG    V         GP+AGPG EG VS+V AG
Sbjct: 61  AGPAAEGWVNDVWAGLK---AGPKAGPGAEGWVSDVKAGLRAGPKAGPGAEGWVSNVKAG 120

Query: 121 PEIERGVSVGAGPRAGP-----------GARRGVDPIVNGVGVGIGV--GYRPGF----- 180
           P +        GPRA P           G RR VDP++NG+G+G+GV  GYR GF     
Sbjct: 121 PTV--------GPRAWPGTEGGVSSSEGGVRRDVDPMINGLGLGLGVDIGYRSGFRAGLG 180

Query: 181 -GPGWNGPGI---GGGLYGDCILGYVCPANERRECNKFAYESCESFNFHPLTASMDLHEV 224
            G  W GPGI   GGG+  +C LGYVCP   RR C+KF+Y +C+++ FHPL ASM LHEV
Sbjct: 181 GGEHWFGPGIGIGGGGVSNECTLGYVCPTYGRRGCDKFSYGNCDTYGFHPLMASMHLHEV 240

BLAST of Spg030209 vs. ExPASy TrEMBL
Match: A0A0A0L7X7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G104850 PE=4 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 1.8e-43
Identity = 126/249 (50.60%), Postives = 155/249 (62.25%), Query Frame = 0

Query: 1   MSSLKYLLLCPFVFLCLSSTFANKLLNSNYGHGSGPDIGFGPRAGPGVEGDAINIGAGPK 60
           M+SLKY LL PF+FLCLS TFAN + N + G      +GFG  + P  +           
Sbjct: 1   MASLKYFLLSPFLFLCLSYTFANGVFNYDDG------LGFGSMSSPTPD----------- 60

Query: 61  AGPWAGPGGKGVSNFGAGPKAGP------GGVSHVGG----GPRAGPGIEGRVSSVGAGP 120
             P AGP  +GVSNFG GPKAGP      GG+S+V      GP+AGPG++  +S+VGAGP
Sbjct: 61  --PSAGPVDRGVSNFGIGPKAGPRAGLGVGGISNVDDGSDPGPKAGPGVKEEMSNVGAGP 120

Query: 121 EIER-GV-SVGAGPRAGPGARRGVDPIVNGVGVGIGV-----------GYRPGFGPGWNG 180
            + + GV S+ AGPRAGP   +GVDPIV G+GVG+GV           G RPG G GW G
Sbjct: 121 RVPKLGVSSIEAGPRAGP---KGVDPIVTGLGVGVGVNLPPIFGGPKMGIRPGPG-GWYG 180

Query: 181 PG-IGGGLYGDCILGYVCPANERRECNKFAYESCESFNFHPLTASMDLHEVEINWAKSKP 226
           PG I    Y +C+LGYVCP N    C K  Y  CES+NF PL+AS +LH+V+INWAKSK 
Sbjct: 181 PGPIIQEPYNNCMLGYVCPTNRPWACGKVGYGLCESYNFRPLSASTELHDVKINWAKSKS 226

BLAST of Spg030209 vs. ExPASy TrEMBL
Match: A0A5A7V4J6 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold18G00010 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 6.8e-35
Identity = 119/240 (49.58%), Postives = 141/240 (58.75%), Query Frame = 0

Query: 1   MSSLKYLLLCPFVFLCLSSTFANKLLNSNYG--HGSGPDIGFGPRAGPGVEGDAINIGAG 60
           M+SLKY LL PF+FLCLS TFA+ + N ++G   GS       P AGPGV+    NIG G
Sbjct: 1   MASLKYFLLSPFLFLCLSYTFADGVFNYDHGLDFGSMSSPTPDPSAGPGVDIGVSNIGIG 60

Query: 61  PKAGPWAGPG-GKGVSNFGAGPKAGPGGVSHVGGGPRAGPGIEGRVSSVGAGPEIERGVS 120
           PKAGP AG G G G+S+    P            GP+AGP   G       G ++  GVS
Sbjct: 61  PKAGPRAGLGIGGGISDVDDEP------------GPKAGPKASG-------GHKL--GVS 120

Query: 121 -VGAGPRAGPGARRGVDPIVNGVGVGIGVGYRPGFG----------PGWNGPG-IGGGLY 180
            + AGPRAGP         VNG GVG+GV   P FG           GW  PG I    Y
Sbjct: 121 GIEAGPRAGPKG-------VNGFGVGVGVDLPPVFGGPKIGLKPGPGGWYRPGPIIQEPY 180

Query: 181 GDCILGYVCPANERRECNKFAYESCESFNFHPLTASMDLHEVEINWAKSKPVAMAQHGKS 226
           G+C+LGYVCP N    C+KFAY  C+S+NFHPL+AS DLHEV+INWAKSKP A AQHG+S
Sbjct: 181 GNCMLGYVCP-NRPWACSKFAYGLCDSYNFHPLSASTDLHEVKINWAKSKPDATAQHGES 211

BLAST of Spg030209 vs. ExPASy TrEMBL
Match: A0A7R9G286 (Hypothetical protein OS=Timema shepardi OX=629360 GN=TSIB3V08_LOCUS8372 PE=4 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.1e-08
Identity = 69/149 (46.31%), Postives = 72/149 (48.32%), Query Frame = 0

Query: 31  GHGSGPDIGFGPRAGPGVEGDAINIGAGPKAGPWAGPGGKGV-----SNFGAGPKAGPGG 90
           G G GP +G GP  GPGV G     G G   GP  GPGG G      S  G GP  GPGG
Sbjct: 558 GPGYGPGVGGGPGYGPGV-GGGPGYGPGVGGGPGYGPGGVGSVPGYGSGVGGGPGYGPGG 617

Query: 91  V--------SHVGGGPRAGPGIEGRVS---SVGAGPEIERGVSVGAGPRAGPGARRGVDP 150
           V          VGGGP  GPG  G       VG GP    G  VG GP  GPG   G   
Sbjct: 618 VGGGPSYGSGGVGGGPGYGPGARGGPGYGPGVGGGPGY--GPGVGGGPGYGPGGAGGGPG 677

Query: 151 IVNGVGVGIGVGYRPGFGPGWNGPGIGGG 164
              GVGVG G G   G GPG+ GPG+GGG
Sbjct: 678 YGPGVGVGPGYGPGVGGGPGY-GPGVGGG 702

BLAST of Spg030209 vs. ExPASy TrEMBL
Match: U3KJI4 (Uncharacterized protein OS=Ficedula albicollis OX=59894 PE=4 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.1e-08
Identity = 60/116 (51.72%), Postives = 63/116 (54.31%), Query Frame = 0

Query: 39  GFGPRAGPGVEGDAINIGAGPKAGPWAGPGGKGVSNFGAGPKAGPGGVSHVGGGPRAGPG 98
           G GP AGPG  G     GAGP AGP AGPG    +  GAGP AGPG  +  G GP AGPG
Sbjct: 1   GAGPGAGPGA-GPGAGPGAGPGAGPGAGPGAGPGAGPGAGPGAGPG--AGPGAGPGAGPG 60

Query: 99  IEGRVSSVGAGPEIERGVSVGAGPRAGPGARRGVDPIVNGVGVGIGVGYRPGFGPG 155
                +  GAGP    G   GAGP AGPGA  G  P   G G G G G  PG GPG
Sbjct: 61  -----AGPGAGPGAGPGAGPGAGPGAGPGAGPGAGP---GAGPGAGPGAGPGAGPG 105