Spg030162 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg030162
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Phytocyanin domain-containing protein
Location	scaffold6: 8254409 .. 8257657 (+)
RNA-Seq Expression	Spg030162
Synteny	Spg030162

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTCTTGTCGTCCCCCTTCCCTTCGTTTCAAAACCAAGCCAGAGGAGGTCGGATACAGGGTCGTCTGAAGCAGAGAAGCCCCACGGCGGCGATGGTGGTAGCGGGAATGGCCAATTTGGTTCAGAGAAACACGAGCACGAACTCTCTCTCAAATCCTTTGTCCCCTTCACACTTTTTCCCCATCCCCATCTCTCACTCCAAATGCTTCTCGCCGTCCCCCTTTTCCCTTTCGTTTCAAAACCAAGCTAGAGGAGGTCGGATACAGGGTCGTCTGAAGCAGAGAAGCCCCACGGCGGCGATGGTGGCAGCAGGAATGGCCGATTTGGTTCAGAGAAGAGCCTTGGAGATCATGATCCCACCTGAGAAACACGAGCACGAAGACGAAGACGACATGCGGCATTCAAAGGCAGATCTGTAAATCGGAATCGATGGCTCGGACAAGAACAGAGTGGTTGCGAGCGGCATTCAAAGGCGTGGTGACGATGGTGACTATGGCTCCACGGTTTGCACTGCAGAGACGGAGAGAAAGAGAAGGGAAAGAGACGGAGACGGAGAGAAAGAGACGAACGACACAAACCCTAAAATTGTGGGAAAGAGACGAATCACGAACGAACGACACAAACCCTAAAATTGTCTATTTAACACAAAAGTGAGAACCGAACCGAACTAGTCGGTTCGGTTCGGTTCGGCCTGGATTTATGTAAAAATCGAACCGATTCGGTTTGGTTCGGTTTTTTCACCCCTAGTTTTCTCTAATTTATACTAGAGATATAGGATAAAACCTTGTAGATTTTATGTAGTAATATATAGGTCTCATTATTTGAATAGTTGGAATTTTCATAAAGGTATAGATCATTTACTATTCGAAGAATTTCGTTATTGTTTCAGAAAGAATATCAAGTCGATAGGGTAGAGCCGACCAACAACTTAGAAATAGCAAGATTTTAGCTATTAATGAACGAAGAGTAGAAGGAGATAATAGTGAAATCATTGCTCTAGAGTTTCGAGCACCTGACCTTTCTGTTTGTTAGTATCGGACTTCTAAGTAGATTAATTGAAATAGAATTTAAATTTCCTCCCATGTTTTATTAATAATAAAGTTGGTAATTAGGCTAAGCTATAAATCTGGCCCTAAATTTTCGAGTTATGTGTCTATTATTTTATTCATATATACTAATTCTAATAGATTTCTATATTTTCCATTTCGTGTTTAATAAGTCTGTGCATGTCAAGTCTTTAGTATAACATTTCTGCCACTAACACATGAACCTATATAAAATTAGTTTTCCATGTAATCCTTAAATAGTATGCTTTAGTATGTCTTGTATACATCAAACTATCACGTTTAATAATAAAAAAAAAAAAAAACTAATAAAAACAAAATTGAAAGTCTAATAACTTATTTATAATTTTTTTAAAGTTTAAAGATCAAATAAACACCCTTAAAAGTTCATACAAATTTTTGTAATTTAACAAAAAATTTAAGATATTGAATGGTAAGATGTTATATACAAACTAGACATATTAGACAGCAGTTAAGCTGATTGAATTTTTTTTTTTTTTTTTTTTTTGTTAAGTTTTTAAAACTCTAATTTTGTAATTCAAGTCAGATTAAAAAGGGAAAAGGTTGAATCTTTCAACAAACCAAACCTACTATTTTTAGTTTTTGAAACTTATTCTTAATTGTTAAAACAAATCATATAATAAAGAAAACCAATAGAAAGCCAAATATATGATGTTAGTTATAAAAGGACTGAAAATCAAAGGCCAAAACAGTTAAGAGAAAATAAAAAGAATTTAAAAAAAAAATGGTTAAGATCAATTGAGGTCCATTCATTCATTGTACTTAAAATAGCTTATTTCCTCAAAAAAATGAGGTAAAGAAAAAAAATAGGGGGAAAGATTGACAAAGTCAAAACAAAGAAGGGAAATCCATTGCCTATTTCTTCTCTTTTTCTCCCCATTGGTGTTGGAGGTGAAGAGCAAACATTAATTTTTGCACACAAAAATTTTCTCTCCCTCTCTTTTTTTTTTTTGTTTTTTTTTTCTTTTTGTCTCTCTCTTAAGCTCCAAAGAGAGAGTGGCCAAAGGGAAAACCAAAATGGACAAAATGTATGTGAGTTTGATGGTTGGAATTTTGAGTTGCTTAGTTTTGATGGGATCTGTCAATGCATTCACTCACATTGTTGGTGGAAGCAATGGTTGGAGAGTGCCTAATAATGTTACCTTTTTCGAACAATGGGCTAAACCTAGAACCTTCGGTGTCGGTGACAGGCTTGGTATGTATTTCTCTTCCTTCTTCCCTCTTGGTAATCATTCAACGTGATTAGCTAATGCCAATGCGGTTGGTTGTCTCTCTCTAAACCATTGAATGCATGATTTTCTAATGTTTGTGTCATGTTTGAATTTGGTGGGCAGTTTTCCCATACCGACCAGGTTGCAACAACTTATTGGCAGTAAATAAGGCGGACTTCGACACATGTGGTCAAGAGAAAGTGATCAACATGTACTTCCTTGGGCCAACCATCCTGAACCTGACCCAGCCAGGTGACTACTACTACTACGATGGAATTGGAAAGCACTGTGAAGCTGGCCAAAAGCTCCACATCAAAGTTGGCACCACCGAGGGATCTTCTGGCTCTGACCCTATGCCATTTAACCTCGAAACCTTTGGAATTCACACCACCCTGAAGATGGGCTTGCCCAATACCATCTCAGCCCCGGCTTCAGCCCCTGAAGCCCTCACAATCCTCGCAACCCCAGCTCCAGCCCCCGGACCATCCAGTGCCGCCGCCGCCACGCCCTTGGCTCACAAATTTGGCTTGGCCTCTGCACCAATGTTAATCATCGCCATGGCTTTCTCCATGTTTCTTTGATTCATCATCTTTAATATATTATTGTTCATCAATGCCTTTGCTTTTTAATAATGTGTATTATTATTGACGAGCTCCCACCAATTTCTTTTCTTGTCTTTTCAATGTTGAAAGGGCTATATATTTCTTGGGATTTTTGGGTCTTCAAATTTATATCTCTTGTTCAAAATTTGTTTCGAAAATAAGGTGGTACTGGTACCCTATATGGAAACTTGTTCTACAAATTAAAGTATTGGAAGTTTTTTTTTTTTTTTCTTTTTGGATTCAGAATTACCTTGTTTTCTAAATAATTTTTTTTTTGGAATATAATATTTGTTGAAATAAAAAAAATCCCATTTGTGTTCTTGTATTAAATATTTAAAATTAATTCCCA

mRNA sequence

ATGCTTCTTGTCGTCCCCCTTCCCTTCGTTTCAAAACCAAGCCAGAGGAGGTCGGATACAGGGTCGTCTGAAGCAGAGAAGCCCCACGGCGGCGATGGTGGTAGCGGGAATGGCCAATTTGGTTCAGAGAAACACGAGCACGAACTCTCTCTCAAATCCTTTGGTCGTCTGAAGCAGAGAAGCCCCACGGCGGCGATGGTGGCAGCAGGAATGGCCGATTTGGTTCAGAGAAGAGCCTTGGAGATCATGATCCCACCTGAGAAACACGAGCACGAAGACGAAGACGACATGCGGCATTCAAAGGCAGATCTAGTGCCTAATAATGTTACCTTTTTCGAACAATGGGCTAAACCTAGAACCTTCGGTGTCGGTGACAGGCTTGTTTTCCCATACCGACCAGGTTGCAACAACTTATTGGCAGTAAATAAGGCGGACTTCGACACATGTGGTCAAGAGAAAGTGATCAACATGTACTTCCTTGGGCCAACCATCCTGAACCTGACCCAGCCAGGTGACTACTACTACTACGATGGAATTGGAAAGCACTGTGAAGCTGGCCAAAAGCTCCACATCAAAGTTGGCACCACCGAGGGATCTTCTGGCTCTGACCCTATGCCATTTAACCTCGAAACCTTTGGAATTCACACCACCCTGAAGATGGGCTTGCCCAATACCATCTCAGCCCCGGCTTCAGCCCCTGAAGCCCTCACAATCCTCGCAACCCCAGCTCCAGCCCCCGGACCATCCAGTGCCGCCGCCGCCACGCCCTTGGCTCACAAATTTGGCTTGGCCTCTGCACCAATGTTAATCATCGCCATGGCTTTCTCCATGTTTCTTTGA

Coding sequence (CDS)

ATGCTTCTTGTCGTCCCCCTTCCCTTCGTTTCAAAACCAAGCCAGAGGAGGTCGGATACAGGGTCGTCTGAAGCAGAGAAGCCCCACGGCGGCGATGGTGGTAGCGGGAATGGCCAATTTGGTTCAGAGAAACACGAGCACGAACTCTCTCTCAAATCCTTTGGTCGTCTGAAGCAGAGAAGCCCCACGGCGGCGATGGTGGCAGCAGGAATGGCCGATTTGGTTCAGAGAAGAGCCTTGGAGATCATGATCCCACCTGAGAAACACGAGCACGAAGACGAAGACGACATGCGGCATTCAAAGGCAGATCTAGTGCCTAATAATGTTACCTTTTTCGAACAATGGGCTAAACCTAGAACCTTCGGTGTCGGTGACAGGCTTGTTTTCCCATACCGACCAGGTTGCAACAACTTATTGGCAGTAAATAAGGCGGACTTCGACACATGTGGTCAAGAGAAAGTGATCAACATGTACTTCCTTGGGCCAACCATCCTGAACCTGACCCAGCCAGGTGACTACTACTACTACGATGGAATTGGAAAGCACTGTGAAGCTGGCCAAAAGCTCCACATCAAAGTTGGCACCACCGAGGGATCTTCTGGCTCTGACCCTATGCCATTTAACCTCGAAACCTTTGGAATTCACACCACCCTGAAGATGGGCTTGCCCAATACCATCTCAGCCCCGGCTTCAGCCCCTGAAGCCCTCACAATCCTCGCAACCCCAGCTCCAGCCCCCGGACCATCCAGTGCCGCCGCCGCCACGCCCTTGGCTCACAAATTTGGCTTGGCCTCTGCACCAATGTTAATCATCGCCATGGCTTTCTCCATGTTTCTTTGA

Protein sequence

MLLVVPLPFVSKPSQRRSDTGSSEAEKPHGGDGGSGNGQFGSEKHEHELSLKSFGRLKQRSPTAAMVAAGMADLVQRRALEIMIPPEKHEHEDEDDMRHSKADLVPNNVTFFEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQEKVINMYFLGPTILNLTQPGDYYYYDGIGKHCEAGQKLHIKVGTTEGSSGSDPMPFNLETFGIHTTLKMGLPNTISAPASAPEALTILATPAPAPGPSSAAAATPLAHKFGLASAPMLIIAMAFSMFL

Homology

[Close]

BLAST of Spg030162 vs. NCBI nr
Match: XP_022982873.1 (umecyanin-like [Cucurbita maxima])

HSP 1 Score: 236.1 bits (601), Expect = 3.6e-58
Identity = 120/177 (67.80%), Postives = 142/177 (80.23%), Query Frame = 0


Query: 105 VPNNVTFFEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQ--EKVINMYFLGP 164
           VP+NVTFF++WAKPRTFGVGD+LVFPYRPG NNLLAV KAD++TCGQ  E VINMY+LGP
Sbjct: 37  VPDNVTFFDEWAKPRTFGVGDKLVFPYRPGANNLLAVKKADYETCGQGEEDVINMYYLGP 96

Query: 165 TILNLTQPGDYYYYDGIGKHCEAGQKLHIKVGTTEGSSGSDPMPFNLETFGIHTTLKMGL 224
           TILN+T PGDYYYYDGIGKHCEAGQKLH++VG+ EGSSGSDP+PFNL+TFGI T      
Sbjct: 97  TILNITHPGDYYYYDGIGKHCEAGQKLHVQVGSKEGSSGSDPLPFNLQTFGIPT----AF 156

Query: 225 PNTISAPASAPEALTILATPAPAPGPSSAAAATPLAHKFGLASAPMLIIAMAFSMFL 280
             T+  PA+AP     + TPA AP  + AAA   +AHKFGLAS+ MLI+ +  S+FL
Sbjct: 157 GTTVEPPAAAP-----VVTPAAAPVVTPAAA---MAHKFGLASSAMLIMPVLLSIFL 201

[Close]

BLAST of Spg030162 vs. NCBI nr
Match: XP_023518646.1 (umecyanin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 224.6 bits (571), Expect = 1.1e-54
Identity = 114/177 (64.41%), Postives = 136/177 (76.84%), Query Frame = 0


Query: 105 VPNNVTFFEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQ--EKVINMYFLGP 164
           VP+NVTFF++WAKPRTFGVGD+LVFPYRPG NNLLAV KAD++TCGQ  E VINMY+LGP
Sbjct: 37  VPDNVTFFDEWAKPRTFGVGDKLVFPYRPGANNLLAVKKADYETCGQGEEDVINMYYLGP 96

Query: 165 TILNLTQPGDYYYYDGIGKHCEAGQKLHIKVGTTEGSSGSDPMPFNLETFGIHTTLKMGL 224
           TILN+TQPGDYYYYDGIGKHCEAGQKLHI+VG+ EG+SGSDP+PFNL+TFGI T      
Sbjct: 97  TILNITQPGDYYYYDGIGKHCEAGQKLHIQVGSKEGTSGSDPLPFNLQTFGIPTAF---- 156

Query: 225 PNTISAPASAPEALTILATPAPAPGPSSAAAATPLAHKFGLASAPMLIIAMAFSMFL 280
             T+  P +A   +T+ A       P     A  +AHKFGLAS+ ML + +  S+FL
Sbjct: 157 -GTMVEPPAAAPVVTLAA-------PRVVTPAAAMAHKFGLASSAML-MPVLLSIFL 200

[Close]

BLAST of Spg030162 vs. NCBI nr
Match: XP_038878156.1 (stellacyanin-like [Benincasa hispida])

HSP 1 Score: 222.6 bits (566), Expect = 4.2e-54
Identity = 117/177 (66.10%), Postives = 132/177 (74.58%), Query Frame = 0


Query: 105 VPNNVTFFEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQEKVINMYFLGPTI 164
           VP N+TFF++W KPRTFGVGDRLVFPYRPG NNLLAV KAD+DTCG+E VI+MYFLGPT+
Sbjct: 34  VPENITFFDEWTKPRTFGVGDRLVFPYRPGANNLLAVKKADYDTCGEEAVIHMYFLGPTV 93

Query: 165 LNLTQPGDYYYYDGIGKHCEAGQKLHIKVGTTEGSSGSDPMPFNLETFGIHTTLKMGLPN 224
           LNLT+PGDYYY+DGIGKHCEAGQKLHI+VG+ EG+SGSDPMPFNLETFGIHT L      
Sbjct: 94  LNLTEPGDYYYFDGIGKHCEAGQKLHIQVGSKEGTSGSDPMPFNLETFGIHTNL------ 153

Query: 225 TISAPASAPEALTILATPAPAPGPSSAAAATPLAHKFGLASAPML--IIAMAFSMFL 280
               PA AP A T  A  A A  PS+A    P          P+L  IIA  FS+FL
Sbjct: 154 ---GPAMAPTAQTQAA--ANATHPSNAFLLPP----------PILPPIIAFLFSIFL 189

[Close]

BLAST of Spg030162 vs. NCBI nr
Match: KAG6600533.1 (hypothetical protein SDJN03_05766, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 221.9 bits (564), Expect = 7.1e-54
Identity = 115/177 (64.97%), Postives = 136/177 (76.84%), Query Frame = 0


Query: 105 VPNNVTFFEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQ--EKVINMYFLGP 164
           VP+NVTFF++WAKPRTFGVGD+LVFPYRPG NNLLAV KAD++TCGQ  E VINMY+LGP
Sbjct: 19  VPDNVTFFDEWAKPRTFGVGDKLVFPYRPGANNLLAVKKADYETCGQGEEDVINMYYLGP 78

Query: 165 TILNLTQPGDYYYYDGIGKHCEAGQKLHIKVGTTEGSSGSDPMPFNLETFGIHTTLKMGL 224
           TILN+T PGDYYYYDGIGKHCEAGQKLHI+VG  EG+SGSDP+PFNL+TFGI T      
Sbjct: 79  TILNITHPGDYYYYDGIGKHCEAGQKLHIQVGLKEGTSGSDPLPFNLQTFGIPT----AF 138

Query: 225 PNTISAPASAPEALTILATPAPAPGPSSAAAATPLAHKFGLASAPMLIIAMAFSMFL 280
             T+  PA+AP  +T+ A     P       A  +AHKFGLAS+ ML + +  S+FL
Sbjct: 139 GTTVEPPAAAP-VVTLDAARVVTP-------AAAMAHKFGLASSAML-MPVLLSIFL 182

[Close]

BLAST of Spg030162 vs. NCBI nr
Match: XP_022942920.1 (stellacyanin-like [Cucurbita moschata] >KAG7031173.1 Early nodulin-like protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 221.9 bits (564), Expect = 7.1e-54
Identity = 115/177 (64.97%), Postives = 136/177 (76.84%), Query Frame = 0


Query: 105 VPNNVTFFEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQ--EKVINMYFLGP 164
           VP+NVTFF++WAKPRTFGVGD+LVFPYRPG NNLLAV KAD++TCGQ  E VINMY+LGP
Sbjct: 37  VPDNVTFFDEWAKPRTFGVGDKLVFPYRPGANNLLAVKKADYETCGQGEEDVINMYYLGP 96

Query: 165 TILNLTQPGDYYYYDGIGKHCEAGQKLHIKVGTTEGSSGSDPMPFNLETFGIHTTLKMGL 224
           TILN+T PGDYYYYDGIGKHCEAGQKLHI+VG  EG+SGSDP+PFNL+TFGI T      
Sbjct: 97  TILNITHPGDYYYYDGIGKHCEAGQKLHIQVGLKEGTSGSDPLPFNLQTFGIPT----AF 156

Query: 225 PNTISAPASAPEALTILATPAPAPGPSSAAAATPLAHKFGLASAPMLIIAMAFSMFL 280
             T+  PA+AP  +T+ A     P       A  +AHKFGLAS+ ML + +  S+FL
Sbjct: 157 GTTVEPPAAAP-VVTLDAARVVTP-------AAAMAHKFGLASSAML-MPVLLSIFL 200

[Close]

BLAST of Spg030162 vs. ExPASy Swiss-Prot
Match: P42849 (Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.3e-12
Identity = 35/96 (36.46%), Postives = 48/96 (50.00%), Query Frame = 0


Query: 106 PNNVTFFEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQEKVINMYFLGPTIL 165
           P++  F+  WA  +TF VGD L F +  G +++  V K  FD C +E  I+     P  +
Sbjct: 14  PSDPKFYITWATGKTFRVGDELEFDFAAGMHDVAVVTKDAFDNCKKENPISHMTTPPVKI 73

Query: 166 NLTQPGDYYYYDGIGKHCEAGQKLHIKVGTTEGSSG 202
            L   G  YY   +G HC  GQKL I V    G+ G
Sbjct: 74  MLNTTGPQYYICTVGDHCRVGQKLSINVVGAGGAGG 109

[Close]

BLAST of Spg030162 vs. ExPASy Swiss-Prot
Match: P00302 (Stellacyanin OS=Toxicodendron vernicifluum OX=4013 PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 5.9e-11
Identity = 32/86 (37.21%), Postives = 47/86 (54.65%), Query Frame = 0


Query: 108 NVTFFEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQEKVINMYFLGPTILNL 167
           +V +  +WA  +TF +GD LVF Y    +N+  V + ++ +C     I  Y  G   +NL
Sbjct: 18  DVDYDWKWASNKTFHIGDVLVFKYDRRFHNVDKVTQKNYQSCNDTTPIASYNTGBBRINL 77

Query: 168 TQPGDYYYYDGIGKHCEAGQKLHIKV 194
              G  YY  G+ KHC+ GQK+HI V
Sbjct: 78  KTVGQKYYICGVPKHCDLGQKVHINV 103

[Close]

BLAST of Spg030162 vs. ExPASy Swiss-Prot
Match: O82081 (Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 5.9e-11
Identity = 44/153 (28.76%), Postives = 74/153 (48.37%), Query Frame = 0


Query: 115 WAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQEKVINMYFLGPTILNLTQPGDYY 174
           WA  +TF VGD LVF Y    ++++ V K +FD+C   K +  +  G +++ LT PG  Y
Sbjct: 43  WAAGQTFAVGDNLVFSYPAAFHDVVEVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRY 102

Query: 175 YYDGIGKHCEAGQKLHIKVGTTEGSSGSDPMPFNLETFG---------IHTTLKMGLPNT 234
           +  G+  HC  G KL + V  T   + + P+P  + +           I   L +     
Sbjct: 103 FICGMPGHCSQGMKLEVNVVPTATVAPTAPLPNTVPSLNAPSPSSVLPIQPLLPLNPVPV 162

Query: 235 ISAPASAP---EALTILATPAPAPGPSSAAAAT 256
           +S  +S P    +L ++   +PA  P++AA  +
Sbjct: 163 LSPSSSTPLPSSSLPLIPPLSPALSPATAAGTS 195

[Close]

BLAST of Spg030162 vs. ExPASy Swiss-Prot
Match: P80728 (Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.0e-10
Identity = 31/89 (34.83%), Postives = 46/89 (51.69%), Query Frame = 0


Query: 112 FEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQEKVINMYFLGPTILNLTQPG 171
           + +WA    F VGD L+F Y    +N+L V++  F +C        Y  G   + L +PG
Sbjct: 20  YAKWASSNKFHVGDSLLFNYNNKFHNVLQVDQEQFKSCNSSSPAASYTSGADSIPLKRPG 79

Query: 172 DYYYYDGIGKHCEAGQKLHIKVGTTEGSS 201
            +Y+  GI  HC+ GQK+ IKV     S+
Sbjct: 80  TFYFLCGIPGHCQLGQKVEIKVDPGSSSA 108

[Close]

BLAST of Spg030162 vs. ExPASy Swiss-Prot
Match: Q41001 (Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.5e-09
Identity = 45/150 (30.00%), Postives = 68/150 (45.33%), Query Frame = 0


Query: 112 FEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQEKVINMYFLGPTILNLTQPG 171
           +  WA  +TF VGD LVF Y  G + +  V ++D+ +C     I+    G T + L + G
Sbjct: 41  YSTWASDKTFAVGDSLVFNYGAGAHTVDEVKESDYKSCTSGNSISTDSTGATTIPLKKAG 100

Query: 172 DYYYYDGIGKHCEAGQKLHIKVGTTEGSSGSDPMPFNLETFGIHTTLKMGLPNTISAPAS 231
            +Y+  G+  H   G KL IKV  + GSS +             ++   G P++   PA 
Sbjct: 101 KHYFICGVPGHSTGGMKLSIKVKASSGSSAAPS--------ATPSSSGKGSPSSDDTPA- 160

Query: 232 APEALTILATPAPAPGPSSAAAATPLAHKF 262
              A T   TP      SSA + +P+   F
Sbjct: 161 ---ATTTTTTPT-KQNESSATSLSPIVALF 177

[Close]

BLAST of Spg030162 vs. ExPASy TrEMBL
Match: A0A6J1J638 (umecyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111481590 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 1.8e-58
Identity = 120/177 (67.80%), Postives = 142/177 (80.23%), Query Frame = 0


Query: 105 VPNNVTFFEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQ--EKVINMYFLGP 164
           VP+NVTFF++WAKPRTFGVGD+LVFPYRPG NNLLAV KAD++TCGQ  E VINMY+LGP
Sbjct: 37  VPDNVTFFDEWAKPRTFGVGDKLVFPYRPGANNLLAVKKADYETCGQGEEDVINMYYLGP 96

Query: 165 TILNLTQPGDYYYYDGIGKHCEAGQKLHIKVGTTEGSSGSDPMPFNLETFGIHTTLKMGL 224
           TILN+T PGDYYYYDGIGKHCEAGQKLH++VG+ EGSSGSDP+PFNL+TFGI T      
Sbjct: 97  TILNITHPGDYYYYDGIGKHCEAGQKLHVQVGSKEGSSGSDPLPFNLQTFGIPT----AF 156

Query: 225 PNTISAPASAPEALTILATPAPAPGPSSAAAATPLAHKFGLASAPMLIIAMAFSMFL 280
             T+  PA+AP     + TPA AP  + AAA   +AHKFGLAS+ MLI+ +  S+FL
Sbjct: 157 GTTVEPPAAAP-----VVTPAAAPVVTPAAA---MAHKFGLASSAMLIMPVLLSIFL 201

[Close]

BLAST of Spg030162 vs. ExPASy TrEMBL
Match: A0A6J1FX52 (stellacyanin-like OS=Cucurbita moschata OX=3662 GN=LOC111447806 PE=4 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 3.4e-54
Identity = 115/177 (64.97%), Postives = 136/177 (76.84%), Query Frame = 0


Query: 105 VPNNVTFFEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQ--EKVINMYFLGP 164
           VP+NVTFF++WAKPRTFGVGD+LVFPYRPG NNLLAV KAD++TCGQ  E VINMY+LGP
Sbjct: 37  VPDNVTFFDEWAKPRTFGVGDKLVFPYRPGANNLLAVKKADYETCGQGEEDVINMYYLGP 96

Query: 165 TILNLTQPGDYYYYDGIGKHCEAGQKLHIKVGTTEGSSGSDPMPFNLETFGIHTTLKMGL 224
           TILN+T PGDYYYYDGIGKHCEAGQKLHI+VG  EG+SGSDP+PFNL+TFGI T      
Sbjct: 97  TILNITHPGDYYYYDGIGKHCEAGQKLHIQVGLKEGTSGSDPLPFNLQTFGIPT----AF 156

Query: 225 PNTISAPASAPEALTILATPAPAPGPSSAAAATPLAHKFGLASAPMLIIAMAFSMFL 280
             T+  PA+AP  +T+ A     P       A  +AHKFGLAS+ ML + +  S+FL
Sbjct: 157 GTTVEPPAAAP-VVTLDAARVVTP-------AAAMAHKFGLASSAML-MPVLLSIFL 200

[Close]

BLAST of Spg030162 vs. ExPASy TrEMBL
Match: A0A6J1ETX6 (stellacyanin-like OS=Cucurbita moschata OX=3662 GN=LOC111437535 PE=4 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 4.2e-52
Identity = 108/175 (61.71%), Postives = 131/175 (74.86%), Query Frame = 0


Query: 105 VPNNVTFFEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQEKVINMYFLGPTI 164
           VP+NVTF+++WAKPRTFGVGD+LVFPYRPG NN++AV KAD++ CG+E VINMY+LGPTI
Sbjct: 33  VPDNVTFYDEWAKPRTFGVGDKLVFPYRPGANNIVAVKKADYEVCGEENVINMYYLGPTI 92

Query: 165 LNLTQPGDYYYYDGIGKHCEAGQKLHIKVGTTEGSSGSDPMPFNLETFGIHTTLKMGLPN 224
           LNLT+ GDYYY+DGIGKHCEAGQKLH++VG  EG+SG+DP+PFNLETFGIHT+L    P 
Sbjct: 93  LNLTEAGDYYYFDGIGKHCEAGQKLHVQVGLKEGTSGADPLPFNLETFGIHTSLD---PT 152

Query: 225 TISAPASAPEALTILATPAPAPGPSSAAAATPLAHKFGLASAPMLIIAMAFSMFL 280
              APA+AP A T           +S A A  L    GLA+     +AM FS  L
Sbjct: 153 QNVAPAAAPTAAT----------KASHANANALLVPAGLAA-----VAMFFSTLL 189

[Close]

BLAST of Spg030162 vs. ExPASy TrEMBL
Match: A0A6J1J846 (stellacyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111483431 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 3.6e-51
Identity = 107/175 (61.14%), Postives = 129/175 (73.71%), Query Frame = 0


Query: 105 VPNNVTFFEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQEKVINMYFLGPTI 164
           VP+NVTF+++WAKPRTFGVGD+LVFPYRPG NN++AV K D++ CG+E VINMY+LGPTI
Sbjct: 36  VPDNVTFYDEWAKPRTFGVGDKLVFPYRPGANNIVAVKKTDYEVCGEENVINMYYLGPTI 95

Query: 165 LNLTQPGDYYYYDGIGKHCEAGQKLHIKVGTTEGSSGSDPMPFNLETFGIHTTLKMGLPN 224
           LNLT+ GDYYY+DGIGKHCEAGQKLH++VG+ EG+SG+DP+PFNLETFGIHT L    P 
Sbjct: 96  LNLTEAGDYYYFDGIGKHCEAGQKLHVQVGSKEGTSGADPLPFNLETFGIHTNLD---PT 155

Query: 225 TISAPASAPEALTILATPAPAPGPSSAAAATPLAHKFGLASAPMLIIAMAFSMFL 280
               PA+AP A T           +  A A  L    GLA+     IAM FS FL
Sbjct: 156 QNVGPAAAPTAAT------KGSNANVNANANALLVPAGLAA-----IAMFFSTFL 196

[Close]

BLAST of Spg030162 vs. ExPASy TrEMBL
Match: A0A0A0KUU5 (Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G051370 PE=4 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 7.9e-51
Identity = 110/178 (61.80%), Postives = 129/178 (72.47%), Query Frame = 0


Query: 105 VPNNVTFFEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQEKVINMYFLGPTI 164
           VP N +FF+QWAKPRTFGVGDRLVFPYR G NNL+ V KAD+DTCG+E+VI MYFLGPT+
Sbjct: 38  VPENDSFFDQWAKPRTFGVGDRLVFPYRAGANNLVTVKKADYDTCGEEEVIYMYFLGPTV 97

Query: 165 LNLTQPGDYYYYDGIGKHCEAGQKLHIKVGTTEGSSGSDPMPFNLETFGIHTTLKMGLPN 224
           +NLT+ GDYYY+DGIGKHCEAGQKLHI+VGT EGSSGSDP+PFNLETFGIHT L      
Sbjct: 98  VNLTKAGDYYYFDGIGKHCEAGQKLHIQVGTKEGSSGSDPLPFNLETFGIHTNL------ 157

Query: 225 TISAPASAPEALTILATPAPAPGPSSAAAATPLAHKFGLASAPM---LIIAMAFSMFL 280
               PA +P+      + + A  PS   A    ++ F L   PM   LII   FS+FL
Sbjct: 158 ---GPALSPQGQMDAESVSQAQSPSGTPAHP--SNAFLLLPTPMLLALIIPTLFSIFL 204

[Close]

BLAST of Spg030162 vs. TAIR 10
Match: AT2G32300.1 (uclacyanin 1 )

HSP 1 Score: 69.7 bits (169), Expect = 4.2e-12
Identity = 44/153 (28.76%), Postives = 74/153 (48.37%), Query Frame = 0


Query: 115 WAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQEKVINMYFLGPTILNLTQPGDYY 174
           WA  +TF VGD LVF Y    ++++ V K +FD+C   K +  +  G +++ LT PG  Y
Sbjct: 43  WAAGQTFAVGDNLVFSYPAAFHDVVEVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRY 102

Query: 175 YYDGIGKHCEAGQKLHIKVGTTEGSSGSDPMPFNLETFG---------IHTTLKMGLPNT 234
           +  G+  HC  G KL + V  T   + + P+P  + +           I   L +     
Sbjct: 103 FICGMPGHCSQGMKLEVNVVPTATVAPTAPLPNTVPSLNAPSPSSVLPIQPLLPLNPVPV 162

Query: 235 ISAPASAP---EALTILATPAPAPGPSSAAAAT 256
           +S  +S P    +L ++   +PA  P++AA  +
Sbjct: 163 LSPSSSTPLPSSSLPLIPPLSPALSPATAAGTS 195

[Close]

BLAST of Spg030162 vs. TAIR 10
Match: AT5G26330.1 (Cupredoxin superfamily protein )

HSP 1 Score: 69.3 bits (168), Expect = 5.5e-12
Identity = 45/167 (26.95%), Postives = 79/167 (47.31%), Query Frame = 0


Query: 112 FEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQEKVINMYFLGPTILNLTQPG 171
           ++ WA  +TF +GD ++F Y P  +N++ V    + +C   K I+ +  G   + LT  G
Sbjct: 40  YKLWASTKTFHIGDTVLFEYNPQFHNVMRVTHPMYRSCNTSKPISTFTTGNDSITLTNHG 99

Query: 172 DYYYYDGIGKHCEAGQKLHIKVGTTEGSSGSDPMPFNLETFGIHTTLKMGLPNTISAPAS 231
            ++++ G+  HC AGQKL + V     S+     P                    ++ +S
Sbjct: 100 HHFFFCGVPGHCLAGQKLDLHVLLPASSTPLSDPP--------------------TSSSS 159

Query: 232 APEALTILATPAPAPGPSSAAAATPLAHKFGLASAPMLIIAMAFSMF 279
           +P + TI A   P P P S AA+ P      + +   L++++AF+ F
Sbjct: 160 SPPSTTIPAAGVPGPSP-SLAASLPSMVTAQIVAVVTLLVSLAFTNF 185

[Close]

BLAST of Spg030162 vs. TAIR 10
Match: AT3G18590.1 (early nodulin-like protein 5 )

HSP 1 Score: 68.6 bits (166), Expect = 9.3e-12
Identity = 43/135 (31.85%), Postives = 64/135 (47.41%), Query Frame = 0


Query: 104 LVPNNVTF---FEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQEKVINMYFL 163
           +VP + T    F QWA    F VGD L F Y    +++L V++ ++  C   K       
Sbjct: 36  IVPKSKTLGDAFNQWASDNRFKVGDTLRFKYTK--DSVLVVSEEEYKKCKATKPQLYSNN 95

Query: 164 GPTILNLTQPGDYYYYDGIGKHCEAGQKLHIKVGTTEGSSGSDPMPFNLETFGIHTTLKM 223
             T+  L +PG +Y+  G+  HCE GQK+ +KV  TE S+ S P            +   
Sbjct: 96  EDTVFKLDRPGLFYFISGVSGHCEKGQKMIVKVMETESSTESPP-----------PSSSS 155

Query: 224 GLPNTISAPASAPEA 236
              ++ S PAS P+A
Sbjct: 156 SSSSSSSLPASTPKA 157

[Close]

BLAST of Spg030162 vs. TAIR 10
Match: AT1G64640.1 (early nodulin-like protein 8 )

HSP 1 Score: 65.9 bits (159), Expect = 6.1e-11
Identity = 30/91 (32.97%), Postives = 48/91 (52.75%), Query Frame = 0


Query: 105 VPNNVTFFEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQEKVINMYFLGPTI 164
           +P +   + +W K  +F +GD L+F Y P  ++L+ V  ++F +C  +  I     G ++
Sbjct: 43  IPIDAKVYSKWPKSHSFKIGDSLLFLYPPSEDSLIQVTPSNFKSCNTKDPILYMNDGNSL 102

Query: 165 LNLTQPGDYYYYDGIGKHCEAGQKLHIKVGT 196
            NLTQ G  Y+      HC   QKL + VGT
Sbjct: 103 FNLTQNGTLYFTSANPGHCTKYQKLLVSVGT 133

[Close]

BLAST of Spg030162 vs. TAIR 10
Match: AT1G48940.1 (early nodulin-like protein 6 )

HSP 1 Score: 65.9 bits (159), Expect = 6.1e-11
Identity = 33/98 (33.67%), Postives = 48/98 (48.98%), Query Frame = 0


Query: 107 NNVTFFEQWAKPRTFGVGDRLVFPYRPGCNNLLAVNKADFDTCGQEKVINMYFLGPTILN 166
           N    F QWA    F VGD + F Y+   +++L V + ++  C   K         T+  
Sbjct: 41  NQSDIFNQWASKNRFKVGDTIRFKYKK--DSVLVVTEDEYKKCQTTKPELYSNHDDTVFK 100

Query: 167 LTQPGDYYYYDGIGKHCEAGQKLHIKVGTTEGSSGSDP 205
           L +PG +Y+  G+  HCE GQK+ IKV   E +  S P
Sbjct: 101 LDRPGLFYFISGVSGHCEQGQKMIIKVMEVESTPQSPP 136

The following BLAST results are available for this feature:

BLAST of Spg030162 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022982873.1	3.6e-58	67.80	umecyanin-like [Cucurbita maxima]	[more]
XP_023518646.1	1.1e-54	64.41	umecyanin-like [Cucurbita pepo subsp. pepo]	[more]
XP_038878156.1	4.2e-54	66.10	stellacyanin-like [Benincasa hispida]	[more]
KAG6600533.1	7.1e-54	64.97	hypothetical protein SDJN03_05766, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022942920.1	7.1e-54	64.97	stellacyanin-like [Cucurbita moschata] >KAG7031173.1 Early nodulin-like protein ...	[more]

BLAST of Spg030162 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42849	5.3e-12	36.46	Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1	[more]
P00302	5.9e-11	37.21	Stellacyanin OS=Toxicodendron vernicifluum OX=4013 PE=1 SV=1	[more]
O82081	5.9e-11	28.76	Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1	[more]
P80728	1.0e-10	34.83	Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1	[more]
Q41001	1.5e-09	30.00	Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1	[more]

BLAST of Spg030162 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J638	1.8e-58	67.80	umecyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111481590 PE=4 SV=1	[more]
A0A6J1FX52	3.4e-54	64.97	stellacyanin-like OS=Cucurbita moschata OX=3662 GN=LOC111447806 PE=4 SV=1	[more]
A0A6J1ETX6	4.2e-52	61.71	stellacyanin-like OS=Cucurbita moschata OX=3662 GN=LOC111437535 PE=4 SV=1	[more]
A0A6J1J846	3.6e-51	61.14	stellacyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111483431 PE=4 SV=1	[more]
A0A0A0KUU5	7.9e-51	61.80	Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G051370...	[more]

BLAST of Spg030162 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32300.1	4.2e-12	28.76	uclacyanin 1	[more]
AT5G26330.1	5.5e-12	26.95	Cupredoxin superfamily protein	[more]
AT3G18590.1	9.3e-12	31.85	early nodulin-like protein 5	[more]
AT1G64640.1	6.1e-11	32.97	early nodulin-like protein 8	[more]
AT1G48940.1	6.1e-11	33.67	early nodulin-like protein 6	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 105..194 e-value: 1.3E-21 score: 78.3
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 105..193
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 110..187 e-value: 9.7E-17 score: 60.8
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 87..195 score: 22.966213
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..49
None	No IPR available	PANTHER	PTHR33021:SF264	OS05G0570900 PROTEIN	coord: 105..217
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 105..217

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg030162.1	Spg030162.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0009055	electron transfer activity