Spg030156 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg030156
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionD-2-hydroxyglutarate dehydrogenase, mitochondrial
Locationscaffold6: 9509853 .. 9510551 (-)
RNA-Seq ExpressionSpg030156
SyntenySpg030156
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TAATTTTAGACTTTGCTCTCATGGCGAATGTAACTATTTATGATTAAAATGATATTTATCTCAAAATTTTAATATGAAATATGAAAAAAAAATCATTCCGACCATTTCAGTTTTCGAAGTTAAACGAGTTTGAGAACGAAGCTTTAGATGATGACGACGACGATGAAGAAGAAAGCTACCAATTATCTCTTCAGATACTCCCGAATTTTTCACTCTGATCAGCGTTTGAATCTAAATTACAAGATTTCGCTTCTCCTTTCAGGTTCATTTTCATGTCTGGATGATTTCATTGATTGATATGCATTGCTAGTTTGTTTACCTGGTTTAATTTCCCCGTCATTTGTAGGAACTTCCTTGTGCTCTAAAATGCCTAACGTTAGGGACAGCACGCCAATCTCACAATATGTGGGTTCAATTCAAGGTTGCAGAAAAGCTTTTCACGCTTCTGCGATCAATCCTTTTAGAATGCCTTGCGGATTTCAGTCCAGACGCTTTGCTTCATTATCGCCAGCGGTTCAGAGGAACCCTTCATTTTCGAGGTTAAATTCTGATGATATCGACTTTTTCCGGAGTATTTTGGGGGAGAAAAATGTAGTTCAGGATGAGGATAGGCTTTTGGATGCAAATACAGATTGGATGCGGAAGTACAGAGGGTCAAGTAAGCTTTTACTACAACCTAGGAGCACAGAAGAGGTTTAA

mRNA sequence

ATGATGACGACGACGATGAAGAAGAAAGCTACCAATTATCTCTTCAGATACTCCCGAATTTTTCACTCTGATCAGCGTTTGAATCTAAATTACAAGATTTCGCTTCTCCTTTCAGGAACTTCCTTGTGCTCTAAAATGCCTAACGTTAGGGACAGCACGCCAATCTCACAATATGTGGGTTCAATTCAAGGTTGCAGAAAAGCTTTTCACGCTTCTGCGATCAATCCTTTTAGAATGCCTTGCGGATTTCAGTCCAGACGCTTTGCTTCATTATCGCCAGCGGTTCAGAGGAACCCTTCATTTTCGAGGTTAAATTCTGATGATATCGACTTTTTCCGGAGTATTTTGGGGGAGAAAAATGTAGTTCAGGATGAGGATAGGCTTTTGGATGCAAATACAGATTGGATGCGGAAGTACAGAGGGTCAAGTAAGCTTTTACTACAACCTAGGAGCACAGAAGAGGTTTAA

Coding sequence (CDS)

ATGATGACGACGACGATGAAGAAGAAAGCTACCAATTATCTCTTCAGATACTCCCGAATTTTTCACTCTGATCAGCGTTTGAATCTAAATTACAAGATTTCGCTTCTCCTTTCAGGAACTTCCTTGTGCTCTAAAATGCCTAACGTTAGGGACAGCACGCCAATCTCACAATATGTGGGTTCAATTCAAGGTTGCAGAAAAGCTTTTCACGCTTCTGCGATCAATCCTTTTAGAATGCCTTGCGGATTTCAGTCCAGACGCTTTGCTTCATTATCGCCAGCGGTTCAGAGGAACCCTTCATTTTCGAGGTTAAATTCTGATGATATCGACTTTTTCCGGAGTATTTTGGGGGAGAAAAATGTAGTTCAGGATGAGGATAGGCTTTTGGATGCAAATACAGATTGGATGCGGAAGTACAGAGGGTCAAGTAAGCTTTTACTACAACCTAGGAGCACAGAAGAGGTTTAA

Protein sequence

MMTTTMKKKATNYLFRYSRIFHSDQRLNLNYKISLLLSGTSLCSKMPNVRDSTPISQYVGSIQGCRKAFHASAINPFRMPCGFQSRRFASLSPAVQRNPSFSRLNSDDIDFFRSILGEKNVVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV
Homology
BLAST of Spg030156 vs. NCBI nr
Match: XP_022136645.1 (D-2-hydroxyglutarate dehydrogenase, mitochondrial isoform X3 [Momordica charantia])

HSP 1 Score: 244.6 bits (623), Expect = 5.7e-61
Identity = 124/155 (80.00%), Postives = 132/155 (85.16%), Query Frame = 0

Query: 1   MMTTTMKKKATNYLFRYSRIFHSDQRLNLNYKISLLLSGTSLCSKMPNVRDSTPISQYVG 60
           MMTT MK KATNY FRYSR FHSDQR NLNYK SLLLSG   CSK P +RD TP S ++G
Sbjct: 1   MMTTMMKNKATNYFFRYSRFFHSDQRFNLNYKNSLLLSGIPSCSKTPGIRDWTPNSPFLG 60

Query: 61  SIQGCRKAFHASAINPFRMPCGFQSRRFASLSPAVQRNPSFSRLNSDDIDFFRSILGEKN 120
           SI+GCR A +ASA++ FR+  GFQ RRF SLS  VQRNPSFSRLNSDDIDFFRSILGEKN
Sbjct: 61  SIRGCRNALNASALDHFRVGGGFQFRRFTSLSSEVQRNPSFSRLNSDDIDFFRSILGEKN 120

Query: 121 VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 156
           VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV
Sbjct: 121 VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 155

BLAST of Spg030156 vs. NCBI nr
Match: XP_022136643.1 (D-2-hydroxyglutarate dehydrogenase, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 244.6 bits (623), Expect = 5.7e-61
Identity = 124/155 (80.00%), Postives = 132/155 (85.16%), Query Frame = 0

Query: 1   MMTTTMKKKATNYLFRYSRIFHSDQRLNLNYKISLLLSGTSLCSKMPNVRDSTPISQYVG 60
           MMTT MK KATNY FRYSR FHSDQR NLNYK SLLLSG   CSK P +RD TP S ++G
Sbjct: 1   MMTTMMKNKATNYFFRYSRFFHSDQRFNLNYKNSLLLSGIPSCSKTPGIRDWTPNSPFLG 60

Query: 61  SIQGCRKAFHASAINPFRMPCGFQSRRFASLSPAVQRNPSFSRLNSDDIDFFRSILGEKN 120
           SI+GCR A +ASA++ FR+  GFQ RRF SLS  VQRNPSFSRLNSDDIDFFRSILGEKN
Sbjct: 61  SIRGCRNALNASALDHFRVGGGFQFRRFTSLSSEVQRNPSFSRLNSDDIDFFRSILGEKN 120

Query: 121 VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 156
           VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV
Sbjct: 121 VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 155

BLAST of Spg030156 vs. NCBI nr
Match: XP_022136644.1 (D-2-hydroxyglutarate dehydrogenase, mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 244.6 bits (623), Expect = 5.7e-61
Identity = 124/155 (80.00%), Postives = 132/155 (85.16%), Query Frame = 0

Query: 1   MMTTTMKKKATNYLFRYSRIFHSDQRLNLNYKISLLLSGTSLCSKMPNVRDSTPISQYVG 60
           MMTT MK KATNY FRYSR FHSDQR NLNYK SLLLSG   CSK P +RD TP S ++G
Sbjct: 1   MMTTMMKNKATNYFFRYSRFFHSDQRFNLNYKNSLLLSGIPSCSKTPGIRDWTPNSPFLG 60

Query: 61  SIQGCRKAFHASAINPFRMPCGFQSRRFASLSPAVQRNPSFSRLNSDDIDFFRSILGEKN 120
           SI+GCR A +ASA++ FR+  GFQ RRF SLS  VQRNPSFSRLNSDDIDFFRSILGEKN
Sbjct: 61  SIRGCRNALNASALDHFRVGGGFQFRRFTSLSSEVQRNPSFSRLNSDDIDFFRSILGEKN 120

Query: 121 VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 156
           VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV
Sbjct: 121 VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 155

BLAST of Spg030156 vs. NCBI nr
Match: XP_022985346.1 (D-2-hydroxyglutarate dehydrogenase, mitochondrial [Cucurbita maxima])

HSP 1 Score: 241.5 bits (615), Expect = 4.8e-60
Identity = 125/155 (80.65%), Postives = 132/155 (85.16%), Query Frame = 0

Query: 1   MMTTTMKKKATNYLFRYSRIFHSDQRLNLNYKISLLLSGTSLCSKMPNVRDSTPISQYVG 60
           M TTTMK+KA +YLFRYSR FHSDQRLNLN+  S LLSGT LC K P +RDSTPISQY  
Sbjct: 2   MTTTTMKRKAASYLFRYSRNFHSDQRLNLNHNNSRLLSGTFLCCKTPIIRDSTPISQY-- 61

Query: 61  SIQGCRKAFHASAINPFRMPCGFQSRRFASLSPAVQRNPSFSRLNSDDIDFFRSILGEKN 120
               CR A+HASAIN FRM  GFQ RRFASLS  VQRNPSFSRLNSDDIDFFRSILGEKN
Sbjct: 62  ----CRDAYHASAINHFRMASGFQFRRFASLSSVVQRNPSFSRLNSDDIDFFRSILGEKN 121

Query: 121 VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 156
           V+QDEDRLLDANTDW+RKYRGSSKLLLQPRSTEEV
Sbjct: 122 VIQDEDRLLDANTDWLRKYRGSSKLLLQPRSTEEV 150

BLAST of Spg030156 vs. NCBI nr
Match: XP_038896937.1 (D-2-hydroxyglutarate dehydrogenase, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 239.6 bits (610), Expect = 1.8e-59
Identity = 122/155 (78.71%), Postives = 133/155 (85.81%), Query Frame = 0

Query: 1   MMTTTMKKKATNYLFRYSRIFHSDQRLNLNYKISLLLSGTSLCSKMPNVRDSTPISQYVG 60
           MMTTTM+KK+ ++LFRYSRIFHSDQ L+LN+  SLL SGTSLC          PIS+Y+G
Sbjct: 2   MMTTTMRKKSASFLFRYSRIFHSDQPLSLNHNNSLLRSGTSLC---------MPISRYIG 61

Query: 61  SIQGCRKAFHASAINPFRMPCGFQSRRFASLSPAVQRNPSFSRLNSDDIDFFRSILGEKN 120
           S +GCRKAF ASAIN FRMPCGFQ RRF SLS  VQRNPSFSRLNSDDIDFFRSILGEKN
Sbjct: 62  SFRGCRKAFQASAINHFRMPCGFQFRRFTSLSSVVQRNPSFSRLNSDDIDFFRSILGEKN 121

Query: 121 VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 156
           VVQDEDRLLDANTDW+RKYRG+SKLLLQPRSTEEV
Sbjct: 122 VVQDEDRLLDANTDWLRKYRGTSKLLLQPRSTEEV 147

BLAST of Spg030156 vs. ExPASy Swiss-Prot
Match: B8B7X6 (Probable D-2-hydroxyglutarate dehydrogenase, mitochondrial OS=Oryza sativa subsp. indica OX=39946 GN=D2HGDH PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 2.5e-19
Identity = 52/103 (50.49%), Postives = 68/103 (66.02%), Query Frame = 0

Query: 61  SIQGCRKAFHASAIN--------PFRMPCGFQSRRFASLSPAVQRNPSFSRLNSDDIDFF 120
           SIQG +++ +    N          RM    Q R F+S +  VQRNP++S LNSDD+ +F
Sbjct: 45  SIQGQQRSLYGFRTNVETDDTQQSARMNFEVQKRSFSSAAAHVQRNPAYSVLNSDDVSYF 104

Query: 121 RSILGEKNVVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 156
           +SILG+  VVQDEDR+  AN DWM KY+GSS+LLL P+ST EV
Sbjct: 105 KSILGDSGVVQDEDRVSVANMDWMGKYKGSSQLLLLPKSTAEV 147

BLAST of Spg030156 vs. ExPASy Swiss-Prot
Match: Q7XI14 (Probable D-2-hydroxyglutarate dehydrogenase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=D2HGDH PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 2.5e-19
Identity = 52/103 (50.49%), Postives = 68/103 (66.02%), Query Frame = 0

Query: 61  SIQGCRKAFHASAIN--------PFRMPCGFQSRRFASLSPAVQRNPSFSRLNSDDIDFF 120
           SIQG +++ +    N          RM    Q R F+S +  VQRNP++S LNSDD+ +F
Sbjct: 45  SIQGQQRSLYGFRTNVETDDTQQSARMNFEVQKRSFSSAAAHVQRNPAYSVLNSDDVSYF 104

Query: 121 RSILGEKNVVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 156
           +SILG+  VVQDEDR+  AN DWM KY+GSS+LLL P+ST EV
Sbjct: 105 KSILGDSGVVQDEDRVSVANMDWMGKYKGSSQLLLLPKSTAEV 147

BLAST of Spg030156 vs. ExPASy Swiss-Prot
Match: O23240 (D-2-hydroxyglutarate dehydrogenase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=D2HGDH PE=1 SV=3)

HSP 1 Score: 91.7 bits (226), Expect = 8.1e-18
Identity = 55/128 (42.97%), Postives = 76/128 (59.38%), Query Frame = 0

Query: 41  SLCSKMPNVRD-STPISQYVGSIQGCRKAFHASAIN---PFRMPC-----GFQSRRF--- 100
           SL S  PN    S  +S +V   +   K F  S  N       PC     G   +++   
Sbjct: 19  SLISSRPNKDSVSRSVSGFVNHYKSKGKLFELSDGNYKTELHHPCISRNVGMLLQQYKCF 78

Query: 101 -ASLSPAVQRNPSFSRLNSDDIDFFRSILGEKNVVQDEDRLLDANTDWMRKYRGSSKLLL 156
            +S +  +QRNP FS L+S D+ +F+ ILGEKNVV+D++RL  ANTDWM KY+GSSKL+L
Sbjct: 79  GSSAASLIQRNPLFSSLDSKDVSYFKEILGEKNVVEDKERLETANTDWMHKYKGSSKLML 138

BLAST of Spg030156 vs. ExPASy Swiss-Prot
Match: P46681 (D-2-hydroxyglutarate--pyruvate transhydrogenase DLD2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DLD2 PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.3e-11
Identity = 33/74 (44.59%), Postives = 52/74 (70.27%), Query Frame = 0

Query: 84  QSRRFASLSPAVQRNPSFSRLNSDDIDFFRSILGEKNVVQ--DEDRLLDANTDWMRKYRG 143
           Q+R  +   P V R+P F +L SDD+++F+SIL E+ +++  + + L   N DWMRKY+G
Sbjct: 41  QTRLTSENYPDVHRDPRFKKLTSDDLNYFKSILSEQEILRASESEDLSFYNEDWMRKYKG 100

Query: 144 SSKLLLQPRSTEEV 156
            SKL+L+P+S E+V
Sbjct: 101 QSKLVLRPKSVEKV 114

BLAST of Spg030156 vs. ExPASy Swiss-Prot
Match: P39976 (D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DLD3 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 4.7e-10
Identity = 32/69 (46.38%), Postives = 45/69 (65.22%), Query Frame = 0

Query: 89  ASLSPAVQRNPSFSRLNSDDIDFFRSILGEKNVV--QDEDRLLDANTDWMRKYRGSSKLL 148
           A   P V+RNP+F  L+S+D+ +FRSIL    ++  Q  + L   N DWM+KYRG S L+
Sbjct: 12  AEAYPKVKRNPNFKVLDSEDLAYFRSILSNDEILNSQAPEELASFNQDWMKKYRGQSNLI 71

Query: 149 LQPRSTEEV 156
           L P ST++V
Sbjct: 72  LLPNSTDKV 80

BLAST of Spg030156 vs. ExPASy TrEMBL
Match: A0A6J1C4I6 (D-2-hydroxyglutarate dehydrogenase, mitochondrial isoform X3 OS=Momordica charantia OX=3673 GN=LOC111008299 PE=4 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.7e-61
Identity = 124/155 (80.00%), Postives = 132/155 (85.16%), Query Frame = 0

Query: 1   MMTTTMKKKATNYLFRYSRIFHSDQRLNLNYKISLLLSGTSLCSKMPNVRDSTPISQYVG 60
           MMTT MK KATNY FRYSR FHSDQR NLNYK SLLLSG   CSK P +RD TP S ++G
Sbjct: 1   MMTTMMKNKATNYFFRYSRFFHSDQRFNLNYKNSLLLSGIPSCSKTPGIRDWTPNSPFLG 60

Query: 61  SIQGCRKAFHASAINPFRMPCGFQSRRFASLSPAVQRNPSFSRLNSDDIDFFRSILGEKN 120
           SI+GCR A +ASA++ FR+  GFQ RRF SLS  VQRNPSFSRLNSDDIDFFRSILGEKN
Sbjct: 61  SIRGCRNALNASALDHFRVGGGFQFRRFTSLSSEVQRNPSFSRLNSDDIDFFRSILGEKN 120

Query: 121 VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 156
           VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV
Sbjct: 121 VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 155

BLAST of Spg030156 vs. ExPASy TrEMBL
Match: A0A6J1C845 (D-2-hydroxyglutarate dehydrogenase, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008299 PE=4 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.7e-61
Identity = 124/155 (80.00%), Postives = 132/155 (85.16%), Query Frame = 0

Query: 1   MMTTTMKKKATNYLFRYSRIFHSDQRLNLNYKISLLLSGTSLCSKMPNVRDSTPISQYVG 60
           MMTT MK KATNY FRYSR FHSDQR NLNYK SLLLSG   CSK P +RD TP S ++G
Sbjct: 1   MMTTMMKNKATNYFFRYSRFFHSDQRFNLNYKNSLLLSGIPSCSKTPGIRDWTPNSPFLG 60

Query: 61  SIQGCRKAFHASAINPFRMPCGFQSRRFASLSPAVQRNPSFSRLNSDDIDFFRSILGEKN 120
           SI+GCR A +ASA++ FR+  GFQ RRF SLS  VQRNPSFSRLNSDDIDFFRSILGEKN
Sbjct: 61  SIRGCRNALNASALDHFRVGGGFQFRRFTSLSSEVQRNPSFSRLNSDDIDFFRSILGEKN 120

Query: 121 VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 156
           VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV
Sbjct: 121 VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 155

BLAST of Spg030156 vs. ExPASy TrEMBL
Match: A0A6J1C4W8 (D-2-hydroxyglutarate dehydrogenase, mitochondrial isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008299 PE=4 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.7e-61
Identity = 124/155 (80.00%), Postives = 132/155 (85.16%), Query Frame = 0

Query: 1   MMTTTMKKKATNYLFRYSRIFHSDQRLNLNYKISLLLSGTSLCSKMPNVRDSTPISQYVG 60
           MMTT MK KATNY FRYSR FHSDQR NLNYK SLLLSG   CSK P +RD TP S ++G
Sbjct: 1   MMTTMMKNKATNYFFRYSRFFHSDQRFNLNYKNSLLLSGIPSCSKTPGIRDWTPNSPFLG 60

Query: 61  SIQGCRKAFHASAINPFRMPCGFQSRRFASLSPAVQRNPSFSRLNSDDIDFFRSILGEKN 120
           SI+GCR A +ASA++ FR+  GFQ RRF SLS  VQRNPSFSRLNSDDIDFFRSILGEKN
Sbjct: 61  SIRGCRNALNASALDHFRVGGGFQFRRFTSLSSEVQRNPSFSRLNSDDIDFFRSILGEKN 120

Query: 121 VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 156
           VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV
Sbjct: 121 VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 155

BLAST of Spg030156 vs. ExPASy TrEMBL
Match: A0A6J1J7W7 (D-2-hydroxyglutarate dehydrogenase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111483383 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.3e-60
Identity = 125/155 (80.65%), Postives = 132/155 (85.16%), Query Frame = 0

Query: 1   MMTTTMKKKATNYLFRYSRIFHSDQRLNLNYKISLLLSGTSLCSKMPNVRDSTPISQYVG 60
           M TTTMK+KA +YLFRYSR FHSDQRLNLN+  S LLSGT LC K P +RDSTPISQY  
Sbjct: 2   MTTTTMKRKAASYLFRYSRNFHSDQRLNLNHNNSRLLSGTFLCCKTPIIRDSTPISQY-- 61

Query: 61  SIQGCRKAFHASAINPFRMPCGFQSRRFASLSPAVQRNPSFSRLNSDDIDFFRSILGEKN 120
               CR A+HASAIN FRM  GFQ RRFASLS  VQRNPSFSRLNSDDIDFFRSILGEKN
Sbjct: 62  ----CRDAYHASAINHFRMASGFQFRRFASLSSVVQRNPSFSRLNSDDIDFFRSILGEKN 121

Query: 121 VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 156
           V+QDEDRLLDANTDW+RKYRGSSKLLLQPRSTEEV
Sbjct: 122 VIQDEDRLLDANTDWLRKYRGSSKLLLQPRSTEEV 150

BLAST of Spg030156 vs. ExPASy TrEMBL
Match: A0A6J1EUK5 (D-2-hydroxyglutarate dehydrogenase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111436053 PE=4 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.2e-59
Identity = 125/155 (80.65%), Postives = 130/155 (83.87%), Query Frame = 0

Query: 1   MMTTTMKKKATNYLFRYSRIFHSDQRLNLNYKISLLLSGTSLCSKMPNVRDSTPISQYVG 60
           M TTTMK+KA +YLFRYSR FHSDQR NLN   S LLSGT LC K P +RDSTPISQY  
Sbjct: 2   MTTTTMKRKAASYLFRYSRNFHSDQRFNLNRNNSRLLSGTFLCCKTPIIRDSTPISQY-- 61

Query: 61  SIQGCRKAFHASAINPFRMPCGFQSRRFASLSPAVQRNPSFSRLNSDDIDFFRSILGEKN 120
               CR A+HASAIN FRM  GFQ RRFASLS  VQRNPSFSRLNSDDIDFFRSILGEKN
Sbjct: 62  ----CRNAYHASAINHFRMASGFQFRRFASLSSVVQRNPSFSRLNSDDIDFFRSILGEKN 121

Query: 121 VVQDEDRLLDANTDWMRKYRGSSKLLLQPRSTEEV 156
           VVQDEDRLLDANTDW+RKYRGSSKLLLQPRSTEEV
Sbjct: 122 VVQDEDRLLDANTDWLRKYRGSSKLLLQPRSTEEV 150

BLAST of Spg030156 vs. TAIR 10
Match: AT4G36400.1 (FAD-linked oxidases family protein )

HSP 1 Score: 91.7 bits (226), Expect = 5.7e-19
Identity = 55/128 (42.97%), Postives = 76/128 (59.38%), Query Frame = 0

Query: 41  SLCSKMPNVRD-STPISQYVGSIQGCRKAFHASAIN---PFRMPC-----GFQSRRF--- 100
           SL S  PN    S  +S +V   +   K F  S  N       PC     G   +++   
Sbjct: 19  SLISSRPNKDSVSRSVSGFVNHYKSKGKLFELSDGNYKTELHHPCISRNVGMLLQQYKCF 78

Query: 101 -ASLSPAVQRNPSFSRLNSDDIDFFRSILGEKNVVQDEDRLLDANTDWMRKYRGSSKLLL 156
            +S +  +QRNP FS L+S D+ +F+ ILGEKNVV+D++RL  ANTDWM KY+GSSKL+L
Sbjct: 79  GSSAASLIQRNPLFSSLDSKDVSYFKEILGEKNVVEDKERLETANTDWMHKYKGSSKLML 138

BLAST of Spg030156 vs. TAIR 10
Match: AT4G36400.2 (FAD-linked oxidases family protein )

HSP 1 Score: 91.7 bits (226), Expect = 5.7e-19
Identity = 55/128 (42.97%), Postives = 76/128 (59.38%), Query Frame = 0

Query: 41  SLCSKMPNVRD-STPISQYVGSIQGCRKAFHASAIN---PFRMPC-----GFQSRRF--- 100
           SL S  PN    S  +S +V   +   K F  S  N       PC     G   +++   
Sbjct: 19  SLISSRPNKDSVSRSVSGFVNHYKSKGKLFELSDGNYKTELHHPCISRNVGMLLQQYKCF 78

Query: 101 -ASLSPAVQRNPSFSRLNSDDIDFFRSILGEKNVVQDEDRLLDANTDWMRKYRGSSKLLL 156
            +S +  +QRNP FS L+S D+ +F+ ILGEKNVV+D++RL  ANTDWM KY+GSSKL+L
Sbjct: 79  GSSAASLIQRNPLFSSLDSKDVSYFKEILGEKNVVEDKERLETANTDWMHKYKGSSKLML 138

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022136645.15.7e-6180.00D-2-hydroxyglutarate dehydrogenase, mitochondrial isoform X3 [Momordica charanti... [more]
XP_022136643.15.7e-6180.00D-2-hydroxyglutarate dehydrogenase, mitochondrial isoform X1 [Momordica charanti... [more]
XP_022136644.15.7e-6180.00D-2-hydroxyglutarate dehydrogenase, mitochondrial isoform X2 [Momordica charanti... [more]
XP_022985346.14.8e-6080.65D-2-hydroxyglutarate dehydrogenase, mitochondrial [Cucurbita maxima][more]
XP_038896937.11.8e-5978.71D-2-hydroxyglutarate dehydrogenase, mitochondrial isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
B8B7X62.5e-1950.49Probable D-2-hydroxyglutarate dehydrogenase, mitochondrial OS=Oryza sativa subsp... [more]
Q7XI142.5e-1950.49Probable D-2-hydroxyglutarate dehydrogenase, mitochondrial OS=Oryza sativa subsp... [more]
O232408.1e-1842.97D-2-hydroxyglutarate dehydrogenase, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
P466813.3e-1144.59D-2-hydroxyglutarate--pyruvate transhydrogenase DLD2 OS=Saccharomyces cerevisiae... [more]
P399764.7e-1046.38D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3 OS=Saccharomyces cerevisiae... [more]
Match NameE-valueIdentityDescription
A0A6J1C4I62.7e-6180.00D-2-hydroxyglutarate dehydrogenase, mitochondrial isoform X3 OS=Momordica charan... [more]
A0A6J1C8452.7e-6180.00D-2-hydroxyglutarate dehydrogenase, mitochondrial isoform X1 OS=Momordica charan... [more]
A0A6J1C4W82.7e-6180.00D-2-hydroxyglutarate dehydrogenase, mitochondrial isoform X2 OS=Momordica charan... [more]
A0A6J1J7W72.3e-6080.65D-2-hydroxyglutarate dehydrogenase, mitochondrial OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1EUK51.2e-5980.65D-2-hydroxyglutarate dehydrogenase, mitochondrial OS=Cucurbita moschata OX=3662 ... [more]
Match NameE-valueIdentityDescription
AT4G36400.15.7e-1942.97FAD-linked oxidases family protein [more]
AT4G36400.25.7e-1942.97FAD-linked oxidases family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 94..155
e-value: 3.7E-12
score: 48.0
NoneNo IPR availablePANTHERPTHR43716D-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIALcoord: 91..155
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 101..155

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg030156.1Spg030156.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006807 nitrogen compound metabolic process
biological_process GO:0009853 photorespiration
cellular_component GO:0005739 mitochondrion
molecular_function GO:0047545 2-hydroxyglutarate dehydrogenase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0051990 (R)-2-hydroxyglutarate dehydrogenase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding