Spg030128 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg030128
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionsubtilisin-like protease SBT3.4
Locationscaffold6: 8843199 .. 8849400 (+)
RNA-Seq ExpressionSpg030128
SyntenySpg030128
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCGGCGCAGGAGAATTGCTGAAGAAACTGCAGAAAGAGAAGGGGATTGTTCGTCATAGGGCAGAGTGGAAGGGGATTGGAAAACCCACGAGTTTTGTTGTGAGTTTCCCACTCTCTCTCGTGTTCCACTTTCTTCTTCCTCTGTTTTTGCCCCTTTCCTTCTTTTCAAACAATATTGAGAGCATCAATGGCGGCGTTCCAGTTACCTGTCCGTGGTGGAGGAAAGGGAAACGGTGACTGAGAAGATTGAAGCGACTGGGAGGAAAACGTATTTGGAAACTGCAGAATCTCTTCAAATCTCTTCAAAGCGACTGGTGGAGGAAGGAAACGGCGTGGGAAATTTGAGAACCTAAACCTAAAATTTTGTATTTAAGTGAAAGTTGAGAACCGAACCGAACCGAGTCGGTTCGGTTCGGGTCGGTCTTGATTTATGTATAAATCGAACCGAACGGAACCGAACCGGCCCGGTTCAGTTCGGTTCGGCGGTTCGGTTCGGTTTTCTTCACCCCTACCTAAGCCATCCTCTTTTATTCTAACCGCACGCAGCCCAAGGAAGCAAATTTTTAGCCTCCAAGCTTTCTCCTTCACGTGTACCAGCCAAGAGTTTCTTTTTTTTTTTTGGCAGCCCTTTTCTTCTTCCTTCGATTTGGCCAGCTCCATTCTCCTCCCTTGATCGGCGATTGGTGCTCGCGGTCAGCATCTCCGGCAAGCTTCTCCGAAGTCTCGACGGGGCGGCGTTCTCCGGCGAACAGGCCTTCCGCGGCAGCGCAGTAGCGTGCGACGACAGCTTGAGGTGGGTTCGATTTTGGCGCAGTTTTGGGTGTGTTTCTCTCGGGTTTTTCATCCTCAAGGTTGGGTTTTTGTTATCCCTTTCGTTCTCCATCTCTCACCCTCGGTTTCAGCAAGTTTCAGACTCAAAGAAGGTTGATTTTCACGTTTTTCCGACGACTGCATAGTGCTGGGCACGAACAGGTTGATTTTCACGTTTTTCAGGCGACTGCATAGTGCTGGGCACGAACAGCCTGTTTTGGGGCATTTTTCGGTGTTCTCCCGACGTTTTTAGTGAGTTTACAGTGTGGGTAAGTTGGATTAAGGGTTTAATTAGTTGGTTGTTATGTTTAATTGAATACCCACTAATTTTTATGTCCGTTCAGCAGTGTTTTCCAGCCAGTTCAATGTAGACACGCGAGCAGCAGCTGTTTGGGGCGTTTTTTAGCATTTTCGGCGAGTTTCAGCAGTGGGTAACTGAGGAAAATCAATTTTTGGTTGATTAATGTAGTTTGGATGAATACCCATTGGGTTTGGCTAATGTTTTCAAGGTACCCATAGCGTTTGGAGGTTTAACCGTCGATTTGGAGCGAAATTGGAACCCGAACAGCAGTTTAAAGTTCTGTTTTGCAGCAGTTTAAAGTTATGTTTTGATTGATTAAAACCTTGGAGTTAAGGAATTTGTTGAAGGTTAATCAAGTGTCTATTTGGCTAAGGTAAGTAGCTATTTGGATTCAAGTTCTATGTCTTAGACTAATTGCCCATTGTTTGTTAATTGAGAATTTAAAGTGGCTAGTTGGATTAAGGTTCCATTTTTAGACCCATTCTATTTGTGAATATTATATGCTAGCATGAATTGAAATCCTTAAGTTGGTCTTTAAGCTTAAGTAAAGTGTTACGTTGGGTTAACTTTTTGGAAGCATGAGTGTGGCATGGAAATGTTATAATGAGTTGTAGCATGTTGAGTTAGCCTTTACGTAAGTAGCTATTTGGGTGCAATTCCTATGTTTTAGGCACATTGCATGACTGATGGAGTTGGTATATAGCCATTGGATGGAGTTCTATGCTTTAGATTTATTTTTCTTTGAAAATTTGATATGCTAGCATGGACAAGATTTAAGCCAGGTGTTTTGCTTGTGAATTCATTTAAGTATGATGAGTTTCATTATAAGGCATGATGTCAGTATGAGGGTCTTAAAGTCAACTTAGCATCTTTTGATTTACTTGGAAGCATGTTTTGTTTGGATAAATTATGATTGGCTATGAATGTCTGCTTGAGTTGTGGATGTGAGATCTGTTGGGTTTTATGCCATAAAGCTCGTAGATAGTAAATGTACATTTGACCGAACATTAATAAAAGTGATGTATTATTCGATGTTTGTATTATATCTTGTCTTATCAACCCTAATCAAATAAACTAAACATCCAAGGCTAATAGAATGAGGCTTGAACTAGTATGTAGGGACATACGGGGATCAATGTTCAAGTTTTAGCTTAAAGGGTCTGTAGTATAGGGATAAGGCTGGGTACCTTATCCTAGTGACACTATGGATACGGCCCGCTTTGTATATTGATACAAACACAATGATCCAACGCGTTCATGTAGTTGACATGCGAGTGGGGGTATCCTGTGCAATGAGTTTGCACAAAGACCGGACCGCGAAATAGTTAACCACTAACTGTAACACCGTTAGTTAGCTCCGGTTTCTATTTCACTAGGATGACCTAGGCAACTTGGCCTTAATCCTGAGTGGATTATGGACTCCTGTCCATGAGGGATTGTCCTTTGATTTGTACGGGTGAGAGTGGCCTGTTCGCCGACTCAATAAGCCTACCATTTTGGGGACAAGACCGAGTGGGGAGTTGGGAACATAGTCTTACAAGATGGAATTCACTCCTTCCCGATATTAGGGTAAGTAGAGGTGTGTTCCCTTAAGTGGTATCTCCGGGTCTTGAACAAGGGCCCTACCCTCTCTATGGCACGAGAGGGATTTCTGTTTGTTGGTTGGACCTCAAACAGGTTGTTCATTAGAGGAGCACTGGTACTTAAGGACCAAGAGGTAGCCCAGGGGTAAAACGGTAATTTGACTTAGCTGGGGTACGAACACACGTGAAGGGCTAACTTGCTGTTGACGGTCGATATCCGTGGACACAGCAATATATCTGCAGTGAGAAGAGTGCAGTTGTGGTTCTATAGTGGAGTGAACCACAGTTAACGAATATTGACTAACTTGGTTAATGAGTTTAGCCAATTAATCTCATATCGTTGGAGCTTTTGATCTGTAAGTCCATTAGGTCCCACCGGTAGCTCACTAGGGGCGTTGAGGTAAAATTCTAAGGGTGAATCGAATATTCGAAGGTGTTCGAATATTAAACTTAAATATGAATTTGATTCATATTTAAAACTATAGGCTATAATTAATGCACATATGATGTATATTAATTCTATAGTTTATATGAGAGAAGTATAATTGAATATATGATATTTAATTATAATATTAGTTAATTGGTGAATTAATTAATATTTCCGTTTTAATTTAATTTAATTAATTTTTTTTAATTAAATTTAATTAAAACCATAGGATAAAAGGAAGGTTTGTATAAATATGTTTATACAAAAACACATTAATATTAAATTAAGGGTTAATTTAATTTAAAAGATTAATTAATATTAATTAATTTTTTCCATCAGTTAACCTAACTGATGGGAACGTGTCTTCCTCTTTTTGTACGTGGGTTGAGAGACGTTTAAGGGTGTGGTTGGCTTCTTTACGCAGATTTATTCATTCAGTTACGCTCTCTCTCGGAAAAAACACACAGAAAAGTTCTCTCATTCCTAGAGTTCTCGGCAATTCTTTTCCTAAGTGTACAGAGACCAAAAATCTCCCCAATTCCCTTAGAGTTTTTGCTCCCACAAGCACCCAACGCGCAATCCCTTTCGAGGATACCGGTGTCATCAAGTGGTGGTGTTCGTGGCTATTTTCCAGCGAGATCAAGGACCTCTTGCTGCTGTTTTTGAGAGCCATTCGGAGAAAATTTGGCAAAACGATCAAGAATCTCTTCAAGGGTAAGTGGTTCTTGACCTAGGATCATTAGGATCCAATTCTAGATTAGATCTAGAATTTTTATTTATTTTTCCAGCATGCTTTCATGTGTTTTACAGCATGTATATGTTTTTTTTTTGCTGTAAAATATTTATTTTTGCCAACGTATCGGTATTCCGATCATGGTTTGTTTTGGGAAAGAGGTCTCTGATATGTTTCCGCTGCGCAAGGGCTATTATCCCTTCAAGATCTGACTCGCTTTGGTTGTAAGGGTTTGGTGTACCGGAAGTGTGAGGACGTTGCGGTTGGGCAGTTTTGAGTATATAAGTGCTTTGTCTTGTTTTTAGGTGACGAGGAAAATTTGGGATTGACCATATGTATGTCGTTATGCTGCCGAATTTTCGGTAGCATTACAGTGGGCAAAGAGACTAAAGTTTAGAAGTATCCCTTAGATTATGATGTGCTAAATCAGCTGTCTGACTAGTATAAACTACAGGTTCTCCAAGCACGTTTTATTATGACTTTTAGAGGACCATGTATGAATGAGAATAGACTTAGAATAGGGACCTTAATCATGTTTTAGGGCCTCAGGTAGACTTAAAGGATCCTTAAGTCTTACGTTAGCTAGAGTGTCTTTCCTTCCCCATCTCAGGGCTGGTAGAACTCAGTTTGTCAGGCACGTTATCGTGCGTTCTATCAGTTGACTGTTAGAGCCTGGCTTCAGGCACGTTATCGTGCGCTCTCTCAGTTCAGGACGGTGTTGAGCTTGTGATCAGACACGTTATTCGTGCGCCAGATGGCACCAGACATGTTTTCATGCGTCAGTTGCTCCACCGTATTTATTATTTATATTTCAGGGGTGTTGTGTTGGGATAGGTGTAGTTCTGCTAGAGGTTGCCAGCTAGCCTCTAGTTTACTTGCCTTAACCGACTGACCCACACAACACTCTAGTTGGCACAGGTTGGGGATAGGTAAACACCCTAGGTAGAACGTCTCATGTACTCAGTTATGGCCCTAGGGTTAGAAACCCGAATGAATGATTTCAGTTAGGTCCCTATAAAAAGACTCAGAATCAAGTTTCATGCTAATAGATTTTATGTTATCAGATCTCATGCTTTCAGTTTCAGCCCTTAAGTGGGCTACTTACTGAGTATATTCTTATATACTCACTCATGCTACATGACTTTCCTTTTCAGGTGGTACTTGACGACATCAGGCGAGCGGCGAGAGCAGAGGCTTGGCTAGGCTATGGGATGCAGCTGCAAATCATGTCTCTAGAAGTCTAGGATCGAGTTTCCTAGTTTCATGCTTTCAGATGTTATTAGTTTTCAACTTTACGAAAATTGTCTATCATGTTTTGACTATTTTGTTATTGTAAACTGACTTATGGTTTGGGCCCAGTTTATTAAATAAAAGATTTTTATCTATGGTCTCTTCCGCCTTTAAAGTTTTAAAAGTTTTACGTTTTCATGCATGTAGTAGCGACTCCTTTGTATGTTTTTATTCCCTTTTTAGTCGGGTCGTTACATTTTTTCTTTATGCTTTATGGTAACTTTTGCTTCTCATTTAATTTGTGGGATTTTGTGAAGTGATCCAGTCGTAAGGTTGAGGCAAGCAAGAACCATCATCGGCAGAAACTTTTAGATGAGTAAATGGGCACAACCAACTTAATTAGTCCCTTGTCTAAAATAAATTATTAGGTGTTTCTGGTACAATGAAGCAATTAGTTTTGCATCGTTGGATGTAAGAGAGAGAAATTGTAAAAGTATTTTGATTTTGTATCCAATGGTTATTAGGGACGATAGACAAATTTGAAGATGTACATCGGTTTTAAAGAGGGAACCTTATTGTACGTGGAACACCTAATAATTTCTCCATGTCTAGAGTTTTGTGAATTTAGCCTAATTAAAATGAAAGGTTTAACTAAACAACCGGACATAGCCACTCCTGGATCCAACATTCTAGCCGCCATTTCACCCCACAGCAGTCTTAGCTACAAAGTGTTTGCAATACAATCAGGAACTTCCATGGCCGCACCTCATATCTCTGGCATTGTGGCTCTTCTTAAATCCCTACATCCTACTTGGTCACCCGCTGCCATTAAATCAACCCTCATCACCACTGGTATGTATGCACAAATAACCCTTTCTTAATTATTCTAACTCTAAACACAAATATCCTTATATTTATACACAAATTAAATGGCAACGCGTGCAAAGGACCCCTTTGGATTGCCCATTTTTGCTGAGGGCTCTCCTCCTAAAGTTGCTAACCCTTTCGACTACGGTGGTGGAGTAGTGGACCCCAAGGCTGTCGCCGACCCCAGGCCTCATCTACGATTTGGACACCACAGATTAAACATCTTACCTTTGTGCCATGGGTTATGA

mRNA sequence

ATGGTCGGCGCAGGAGAATTGCTGAAGAAACTGCAGAAAGAGAAGGGGATTGTTCGTCATAGGGCAGAGTGGAAGGGGATTGGAAAACCCACGAGTTTTGTTGTGAGTTTCCCACTCTCTCTCGTGTTCCACTTTCTTCTTCCTCTGTTTTTGCCCCTTTCCTTCTTTTCAAACAATATTGAGAGCATCAATGGCGGCGTTCCAGTTACCTCTCCATTCTCCTCCCTTGATCGGCGATTGGTGCTCGCGGTCAGCATCTCCGGCAAGCTTCTCCGAAGTCTCGACGGGGCGGCGTTCTCCGGCGAACAGGCCTTCCGCGGCAGCGCAGTAGCGTGCGACGACAGCTTGAGGCGACTGCATAGTGCTGGGCACGAACAGCCTGTTTTGGGGCATTTTTCGGTGTTCTCCCGACGTTTTTATTTGGATGAATACCCATTGGGTTTGGCTAATGTTTTCAAGGTACCCATAGCGTTTGGAGGTTTAACCGTCGATTTGGAGCGAAATTGGAACCCGAACAGCAGTTTAAAATTTATTCATTCAGTTACGCTCTCTCTCGGAAAAAACACACAGAAAAGTTCTCTCATTCCTAGAGTTCTCGGCAATTCTTTTCCTAAGTGTACAGAGACCAAAAATCTCCCCAATTCCCTTAGAGTTTTTGCTCCCACAAGCACCCAACGCGCAATCCCTTTCGAGGATACCGGTGTCATCAAGTGGTGGTGTTCGTGGCTATTTTCCAGCGAGATCAAGGACCTCTTGCTGCTGTTTTTGAGAGCCATTCGGAGAAAATTTGGCAAAACGATCAAGAATCTCTTCAAGGGTGACGAGGAAAATTTGGGATTGACCATATGCACGTTATCGTGCGCTCTCTCAGTTCAGGACGGTGTTGAGCTTGTGATCAGACACGTTATTCGTGCGCCAGATGGCACCAGACATGTGGTACTTGACGACATCAGGCGAGCGGCGAGAGCAGAGGCTTGGCTAGGCTATGGGATGCAGCTGCAAATCATCCTAATTAAAATGAAAGGTTTAACTAAACAACCGGACATAGCCACTCCTGGATCCAACATTCTAGCCGCCATTTCACCCCACAGCAGTCTTAGCTACAAAGTGTTTGCAATACAATCAGGAACTTCCATGGCCGCACCTCATATCTCTGGCATTGTGGCTCTTCTTAAATCCCTACATCCTACTTGGTCACCCGCTGCCATTAAATCAACCCTCATCACCACTGTTGCTAACCCTTTCGACTACGGTGGTGGAGTAGTGGACCCCAAGGCTGTCGCCGACCCCAGGCCTCATCTACGATTTGGACACCACAGATTAAACATCTTACCTTTGTGCCATGGGTTATGA

Coding sequence (CDS)

ATGGTCGGCGCAGGAGAATTGCTGAAGAAACTGCAGAAAGAGAAGGGGATTGTTCGTCATAGGGCAGAGTGGAAGGGGATTGGAAAACCCACGAGTTTTGTTGTGAGTTTCCCACTCTCTCTCGTGTTCCACTTTCTTCTTCCTCTGTTTTTGCCCCTTTCCTTCTTTTCAAACAATATTGAGAGCATCAATGGCGGCGTTCCAGTTACCTCTCCATTCTCCTCCCTTGATCGGCGATTGGTGCTCGCGGTCAGCATCTCCGGCAAGCTTCTCCGAAGTCTCGACGGGGCGGCGTTCTCCGGCGAACAGGCCTTCCGCGGCAGCGCAGTAGCGTGCGACGACAGCTTGAGGCGACTGCATAGTGCTGGGCACGAACAGCCTGTTTTGGGGCATTTTTCGGTGTTCTCCCGACGTTTTTATTTGGATGAATACCCATTGGGTTTGGCTAATGTTTTCAAGGTACCCATAGCGTTTGGAGGTTTAACCGTCGATTTGGAGCGAAATTGGAACCCGAACAGCAGTTTAAAATTTATTCATTCAGTTACGCTCTCTCTCGGAAAAAACACACAGAAAAGTTCTCTCATTCCTAGAGTTCTCGGCAATTCTTTTCCTAAGTGTACAGAGACCAAAAATCTCCCCAATTCCCTTAGAGTTTTTGCTCCCACAAGCACCCAACGCGCAATCCCTTTCGAGGATACCGGTGTCATCAAGTGGTGGTGTTCGTGGCTATTTTCCAGCGAGATCAAGGACCTCTTGCTGCTGTTTTTGAGAGCCATTCGGAGAAAATTTGGCAAAACGATCAAGAATCTCTTCAAGGGTGACGAGGAAAATTTGGGATTGACCATATGCACGTTATCGTGCGCTCTCTCAGTTCAGGACGGTGTTGAGCTTGTGATCAGACACGTTATTCGTGCGCCAGATGGCACCAGACATGTGGTACTTGACGACATCAGGCGAGCGGCGAGAGCAGAGGCTTGGCTAGGCTATGGGATGCAGCTGCAAATCATCCTAATTAAAATGAAAGGTTTAACTAAACAACCGGACATAGCCACTCCTGGATCCAACATTCTAGCCGCCATTTCACCCCACAGCAGTCTTAGCTACAAAGTGTTTGCAATACAATCAGGAACTTCCATGGCCGCACCTCATATCTCTGGCATTGTGGCTCTTCTTAAATCCCTACATCCTACTTGGTCACCCGCTGCCATTAAATCAACCCTCATCACCACTGTTGCTAACCCTTTCGACTACGGTGGTGGAGTAGTGGACCCCAAGGCTGTCGCCGACCCCAGGCCTCATCTACGATTTGGACACCACAGATTAAACATCTTACCTTTGTGCCATGGGTTATGA

Protein sequence

MVGAGELLKKLQKEKGIVRHRAEWKGIGKPTSFVVSFPLSLVFHFLLPLFLPLSFFSNNIESINGGVPVTSPFSSLDRRLVLAVSISGKLLRSLDGAAFSGEQAFRGSAVACDDSLRRLHSAGHEQPVLGHFSVFSRRFYLDEYPLGLANVFKVPIAFGGLTVDLERNWNPNSSLKFIHSVTLSLGKNTQKSSLIPRVLGNSFPKCTETKNLPNSLRVFAPTSTQRAIPFEDTGVIKWWCSWLFSSEIKDLLLLFLRAIRRKFGKTIKNLFKGDEENLGLTICTLSCALSVQDGVELVIRHVIRAPDGTRHVVLDDIRRAARAEAWLGYGMQLQIILIKMKGLTKQPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSTLITTVANPFDYGGGVVDPKAVADPRPHLRFGHHRLNILPLCHGL
Homology
BLAST of Spg030128 vs. NCBI nr
Match: XP_022159381.1 (subtilisin-like protease SBT3.4, partial [Momordica charantia])

HSP 1 Score: 125.9 bits (315), Expect = 8.5e-25
Identity = 78/160 (48.75%), Postives = 94/160 (58.75%), Query Frame = 0

Query: 302 VIRAPD-GTR--HVVLD---DIRRAARAEAWLGYGMQLQIILIKMKGLTK------QPDI 361
           ++  PD GT+  H  LD    + R  RA   +G  +   +     +G         +PDI
Sbjct: 361 IVLDPDVGTKFFHYXLDTSNPMVRIGRAXTIVGKPISTNVAFFSSRGPNSASPAILKPDI 420

Query: 362 ATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSTLIT 421
           A PGSNILAAISP +  S K FA QSGTSMAAPHISGIVALLKSLHPTWSPAAI+S + T
Sbjct: 421 AAPGSNILAAISPRNPFSDKGFAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSAJST 480

Query: 422 T-------------------VANPFDYGGGVVDPKAVADP 431
           T                   +A+PFDYGGG+V+P A ADP
Sbjct: 481 TARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADP 520

BLAST of Spg030128 vs. NCBI nr
Match: XP_023552781.1 (subtilisin-like protease SBT3.7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 116.7 bits (291), Expect = 5.2e-22
Identity = 64/104 (61.54%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDIA PGSNI+AA+ P+   S K FA  SGTSMAAPHISGIVAL+KSL PTWSPAAIKS
Sbjct: 509 KPDIAAPGSNIIAAVPPNHKGSDKGFAAMSGTSMAAPHISGIVALIKSLRPTWSPAAIKS 568

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            LITT                   VA+PFDYGGGVVD  A  DP
Sbjct: 569 ALITTARVEDPSGLPIFAEGSPPKVADPFDYGGGVVDANAAVDP 612

BLAST of Spg030128 vs. NCBI nr
Match: XP_004146562.1 (subtilisin-like protease SBT3.6 [Cucumis sativus] >KGN53334.1 hypothetical protein Csa_014762 [Cucumis sativus])

HSP 1 Score: 115.5 bits (288), Expect = 1.2e-21
Identity = 60/104 (57.69%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDI+ PGSNILAA+SPH   + K F + SGTSMA PHIS IVALLKS+HPTWSPAAIKS
Sbjct: 516 KPDISAPGSNILAAVSPHHIFNEKGFMLLSGTSMATPHISAIVALLKSVHPTWSPAAIKS 575

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            L+TT                   +A+PFDYGGG+VD  A  DP
Sbjct: 576 ALMTTARTEVSPGLPIFAEGTPPKMADPFDYGGGIVDANAAVDP 619

BLAST of Spg030128 vs. NCBI nr
Match: TYK16638.1 (subtilisin-like protease SBT3.7 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 114.4 bits (285), Expect = 2.6e-21
Identity = 59/104 (56.73%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDI+ PGSNIL+A+SPH   + K F++ SGTSMA PH+S IVALLKS+HPTWSPAAIKS
Sbjct: 415 KPDISAPGSNILSAVSPHYFFNEKGFSLMSGTSMATPHVSAIVALLKSVHPTWSPAAIKS 474

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            L+TT                   VA+PFDYG GVVD  A  DP
Sbjct: 475 ALMTTARTEVSPGLPIFADGTPPKVADPFDYGAGVVDANAAVDP 518

BLAST of Spg030128 vs. NCBI nr
Match: XP_016901145.1 (PREDICTED: subtilisin-like protease SBT3.7 isoform X2 [Cucumis melo])

HSP 1 Score: 114.4 bits (285), Expect = 2.6e-21
Identity = 59/104 (56.73%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDI+ PGSNIL+A+SPH   + K F++ SGTSMA PH+S IVALLKS+HPTWSPAAIKS
Sbjct: 441 KPDISAPGSNILSAVSPHYFFNEKGFSLMSGTSMATPHVSAIVALLKSVHPTWSPAAIKS 500

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            L+TT                   VA+PFDYG GVVD  A  DP
Sbjct: 501 ALMTTARTEVSPGLPIFADGTPPKVADPFDYGAGVVDANAAVDP 544

BLAST of Spg030128 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.6e-21
Identity = 54/104 (51.92%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDIA PG NILAAISP+SS++   FA+ SGTSMA P +SG+V LLKSLHP WSP+AIKS
Sbjct: 502 KPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLLKSLHPDWSPSAIKS 561

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            ++TT                   +A+PFDYGGG+++P+    P
Sbjct: 562 AIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKP 605

BLAST of Spg030128 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 1.0e-20
Identity = 53/104 (50.96%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDIA PG +ILAA SP+ +L+   F ++SGTSMAAP ISG++ALLKSLHP WSPAA +S
Sbjct: 519 KPDIAAPGVSILAATSPNDTLNAGGFVMRSGTSMAAPVISGVIALLKSLHPDWSPAAFRS 578

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            ++TT                   V +PFDYGGG+V+P+  A+P
Sbjct: 579 AIVTTAWRTDPFGEQIAAESSSLKVPDPFDYGGGLVNPEKAAEP 622

BLAST of Spg030128 vs. ExPASy Swiss-Prot
Match: Q9MAP4 (Subtilisin-like protease SBT3.2 OS=Arabidopsis thaliana OX=3702 GN=SBT3.2 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.3e-20
Identity = 54/104 (51.92%), Postives = 71/104 (68.27%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDIA PG NILAA SP+ +   K FA++SGTSM+AP ++GIVALLKS+HP WSPAAI+S
Sbjct: 480 KPDIAAPGVNILAATSPNDTFYDKGFAMKSGTSMSAPVVAGIVALLKSVHPHWSPAAIRS 539

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            ++TT                   +A+PFDYGGGVV+ +  A+P
Sbjct: 540 AIVTTAWRTDPSGEPIFADGSNRKLADPFDYGGGVVNSEKAANP 583

BLAST of Spg030128 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.7e-20
Identity = 52/104 (50.00%), Postives = 69/104 (66.35%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDIA PG  ILAA SP+ +L+   FA+ SGTSMA P ISG++ALLK+LHP WSPAA +S
Sbjct: 523 KPDIAAPGVRILAATSPNDTLNVGGFAMLSGTSMATPVISGVIALLKALHPEWSPAAFRS 582

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            ++TT                   V++PFDYGGG+V+P+  A+P
Sbjct: 583 AIVTTAWRTDPFGEQIFAEGSSRKVSDPFDYGGGIVNPEKAAEP 626

BLAST of Spg030128 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 2.3e-20
Identity = 53/104 (50.96%), Postives = 68/104 (65.38%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDI  PG +ILAA SP S+ S   F I +GTSMAAP ++G+VALLK+LHP WSPAA +S
Sbjct: 520 KPDIGAPGVSILAATSPDSNSSVGGFDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRS 579

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            ++TT                   VA+PFDYGGG+V+P+  ADP
Sbjct: 580 AIVTTAWRTDPFGEQIFAEGSSRKVADPFDYGGGIVNPEKAADP 623

BLAST of Spg030128 vs. ExPASy TrEMBL
Match: A0A6J1E3R4 (subtilisin-like protease SBT3.4 OS=Momordica charantia OX=3673 GN=LOC111025797 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 3.2e-25
Identity = 79/160 (49.38%), Postives = 94/160 (58.75%), Query Frame = 0

Query: 302 VIRAPD-GTR--HVVLD---DIRRAARAEAWLGYGMQLQIILIKMKGLTK------QPDI 361
           ++  PD GT+  H  LD    + R  RA   +G  +   +     +G         +PDI
Sbjct: 361 IVLDPDVGTKFFHYXLDTSNPMVRIGRAXTIVGKPISTNVAFFSSRGPNSASPAILKPDI 420

Query: 362 ATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKSTLIT 421
           A PGSNILAAISP +  S K FA QSGTSMAAPHISGIVALLKSLHPTWSPAAI+S L T
Sbjct: 421 AAPGSNILAAISPRNPFSDKGFAFQSGTSMAAPHISGIVALLKSLHPTWSPAAIRSALST 480

Query: 422 T-------------------VANPFDYGGGVVDPKAVADP 431
           T                   +A+PFDYGGG+V+P A ADP
Sbjct: 481 TARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADP 520

BLAST of Spg030128 vs. ExPASy TrEMBL
Match: A0A0A0KWS6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G047950 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 5.6e-22
Identity = 60/104 (57.69%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDI+ PGSNILAA+SPH   + K F + SGTSMA PHIS IVALLKS+HPTWSPAAIKS
Sbjct: 516 KPDISAPGSNILAAVSPHHIFNEKGFMLLSGTSMATPHISAIVALLKSVHPTWSPAAIKS 575

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            L+TT                   +A+PFDYGGG+VD  A  DP
Sbjct: 576 ALMTTARTEVSPGLPIFAEGTPPKMADPFDYGGGIVDANAAVDP 619

BLAST of Spg030128 vs. ExPASy TrEMBL
Match: A0A5A7TRR2 (Subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G001180 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.2e-21
Identity = 59/104 (56.73%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDI+ PGSNIL+A+SPH   + K F++ SGTSMA PH+S IVALLKS+HPTWSPAAIKS
Sbjct: 500 KPDISAPGSNILSAVSPHYFFNEKGFSLMSGTSMATPHVSAIVALLKSVHPTWSPAAIKS 559

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            L+TT                   VA+PFDYG GVVD  A  DP
Sbjct: 560 ALMTTARTEVSPGLPIFADGTPPKVADPFDYGAGVVDANAAVDP 603

BLAST of Spg030128 vs. ExPASy TrEMBL
Match: A0A5D3CZC2 (Subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004660 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.2e-21
Identity = 59/104 (56.73%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDI+ PGSNIL+A+SPH   + K F++ SGTSMA PH+S IVALLKS+HPTWSPAAIKS
Sbjct: 415 KPDISAPGSNILSAVSPHYFFNEKGFSLMSGTSMATPHVSAIVALLKSVHPTWSPAAIKS 474

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            L+TT                   VA+PFDYG GVVD  A  DP
Sbjct: 475 ALMTTARTEVSPGLPIFADGTPPKVADPFDYGAGVVDANAAVDP 518

BLAST of Spg030128 vs. ExPASy TrEMBL
Match: A0A7J6FVA3 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_020207 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.2e-21
Identity = 59/104 (56.73%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDIA PG NILAA SPH   +   + + SGTSM+ PHISGIVALLK+LHPTWSPAAIKS
Sbjct: 485 KPDIAAPGVNILAATSPHDQFADGEYVMHSGTSMSTPHISGIVALLKALHPTWSPAAIKS 544

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            LITT                   +A+PFDYGGG+V+P A A+P
Sbjct: 545 ALITTARRTSPSGSPIIAEGSPQKLADPFDYGGGIVNPNAAAEP 588

BLAST of Spg030128 vs. TAIR 10
Match: AT4G10520.1 (Subtilase family protein )

HSP 1 Score: 105.5 bits (262), Expect = 1.1e-22
Identity = 54/104 (51.92%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDIA PG NILAAISP+SS++   FA+ SGTSMA P +SG+V LLKSLHP WSP+AIKS
Sbjct: 502 KPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLLKSLHPDWSPSAIKS 561

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            ++TT                   +A+PFDYGGG+++P+    P
Sbjct: 562 AIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKP 605

BLAST of Spg030128 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 102.8 bits (255), Expect = 7.2e-22
Identity = 53/104 (50.96%), Postives = 70/104 (67.31%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDIA PG +ILAA SP+ +L+   F ++SGTSMAAP ISG++ALLKSLHP WSPAA +S
Sbjct: 519 KPDIAAPGVSILAATSPNDTLNAGGFVMRSGTSMAAPVISGVIALLKSLHPDWSPAAFRS 578

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            ++TT                   V +PFDYGGG+V+P+  A+P
Sbjct: 579 AIVTTAWRTDPFGEQIAAESSSLKVPDPFDYGGGLVNPEKAAEP 622

BLAST of Spg030128 vs. TAIR 10
Match: AT1G32970.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 102.4 bits (254), Expect = 9.4e-22
Identity = 54/104 (51.92%), Postives = 71/104 (68.27%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDIA PG NILAA SP+ +   K FA++SGTSM+AP ++GIVALLKS+HP WSPAAI+S
Sbjct: 480 KPDIAAPGVNILAATSPNDTFYDKGFAMKSGTSMSAPVVAGIVALLKSVHPHWSPAAIRS 539

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            ++TT                   +A+PFDYGGGVV+ +  A+P
Sbjct: 540 AIVTTAWRTDPSGEPIFADGSNRKLADPFDYGGGVVNSEKAANP 583

BLAST of Spg030128 vs. TAIR 10
Match: AT1G32960.1 (Subtilase family protein )

HSP 1 Score: 102.1 bits (253), Expect = 1.2e-21
Identity = 52/104 (50.00%), Postives = 69/104 (66.35%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDIA PG  ILAA SP+ +L+   FA+ SGTSMA P ISG++ALLK+LHP WSPAA +S
Sbjct: 523 KPDIAAPGVRILAATSPNDTLNVGGFAMLSGTSMATPVISGVIALLKALHPEWSPAAFRS 582

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            ++TT                   V++PFDYGGG+V+P+  A+P
Sbjct: 583 AIVTTAWRTDPFGEQIFAEGSSRKVSDPFDYGGGIVNPEKAAEP 626

BLAST of Spg030128 vs. TAIR 10
Match: AT1G32940.1 (Subtilase family protein )

HSP 1 Score: 101.7 bits (252), Expect = 1.6e-21
Identity = 53/104 (50.96%), Postives = 68/104 (65.38%), Query Frame = 0

Query: 346 QPDIATPGSNILAAISPHSSLSYKVFAIQSGTSMAAPHISGIVALLKSLHPTWSPAAIKS 405
           +PDI  PG +ILAA SP S+ S   F I +GTSMAAP ++G+VALLK+LHP WSPAA +S
Sbjct: 520 KPDIGAPGVSILAATSPDSNSSVGGFDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRS 579

Query: 406 TLITT-------------------VANPFDYGGGVVDPKAVADP 431
            ++TT                   VA+PFDYGGG+V+P+  ADP
Sbjct: 580 AIVTTAWRTDPFGEQIFAEGSSRKVADPFDYGGGIVNPEKAADP 623

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022159381.18.5e-2548.75subtilisin-like protease SBT3.4, partial [Momordica charantia][more]
XP_023552781.15.2e-2261.54subtilisin-like protease SBT3.7 [Cucurbita pepo subsp. pepo][more]
XP_004146562.11.2e-2157.69subtilisin-like protease SBT3.6 [Cucumis sativus] >KGN53334.1 hypothetical prote... [more]
TYK16638.12.6e-2156.73subtilisin-like protease SBT3.7 isoform X1 [Cucumis melo var. makuwa][more]
XP_016901145.12.6e-2156.73PREDICTED: subtilisin-like protease SBT3.7 isoform X2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9ZSB01.6e-2151.92Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 S... [more]
F4HPF11.0e-2050.96Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 S... [more]
Q9MAP41.3e-2051.92Subtilisin-like protease SBT3.2 OS=Arabidopsis thaliana OX=3702 GN=SBT3.2 PE=2 S... [more]
Q9MAP51.7e-2050.00Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 S... [more]
Q9MAP72.3e-2050.96Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1E3R43.2e-2549.38subtilisin-like protease SBT3.4 OS=Momordica charantia OX=3673 GN=LOC111025797 P... [more]
A0A0A0KWS65.6e-2257.69Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G047950 PE=3 SV=1[more]
A0A5A7TRR21.2e-2156.73Subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A5D3CZC21.2e-2156.73Subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A7J6FVA31.2e-2156.73Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_020207 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G10520.11.1e-2251.92Subtilase family protein [more]
AT1G32950.17.2e-2250.96Subtilase family protein [more]
AT1G32970.19.4e-2251.92Subtilisin-like serine endopeptidase family protein [more]
AT1G32960.11.2e-2150.00Subtilase family protein [more]
AT1G32940.11.6e-2150.96Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 343..438
e-value: 3.4E-17
score: 62.6
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 346..430
e-value: 2.0E-28
score: 101.4
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 344..428
NoneNo IPR availablePANTHERPTHR10795:SF377OS09G0530800 PROTEINcoord: 346..430
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 346..430
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 376..386

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg030128.1Spg030128.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity