Spg030114 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg030114
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRNase H domain-containing protein
Locationscaffold6: 13195783 .. 13196842 (+)
RNA-Seq ExpressionSpg030114
SyntenySpg030114
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGAAGAGAAATCTAGCGAAAGAGGACGAGATCATTTGGAGGAAGGATCCAAAGGGGAAGTTCTCTGTTAAGAGCGCCTATAACTTAGCTATCCAAATTGAGGCTCAAGGTTCAGCGTCTGAATCTGATTCCACAGAGTAAAAAAAGCTTTGGAAATCGGTTTGGAAGTTGGATTGTAGGCCTAAAGTGAAGATCAACCTTTGGTAAGCTATGAGCAATGCTCTCCCCACAGTGGAGAACATAAAGAAAAGAGGAATTCTTACTAATGATCGGTGTTATTTGTGCAGGAATAAAAAAAAAAGATGTGGAACATGTGTTCTGGAACTACAAAGTGGTAAGATCTTTGTGGAAGATTTTATTCCCTAAACTTAATATATTATTCCACAATTGCAAGAGTTGGTGGAAGTTCAAAGACTTCTGGGATGGAGCCTCGCGAATTCTGGATGCTAAAGACGCAAGTGCGGCTAGCCACTTAATTTGGGAAATTTGGCAGAAGAGGAACAATTTGAAGCAGTCAAAAGGCAACAAAGACACAAAGCAAATTTTGCAAGACATTCTCAGTGCAGCACACAGATTCATGATCGAAAACGAGTGGGAGAAGACTAACCATGGCAAAAGGTCAGAGACCCTCCCGAGTCACGGGCGTTGGATCCCGCCGGGCAGTTCGCAATGGAAGCTGAATGTGGACGTAGCCTGGTTTGAAGCCTCAAACTCAAGCGGAGTGGGGTGGATAGTCCGGGACTCCGAAGGTTCTCTGATAGGAGCGGGCTCCAAGAAGACAAGTGGAAGATTAAAGATAAAGATGCTTGAAGCCATGACCGTGGTCGAAGGTTTCTCTATTGTATCGGAAAGATTCAGAAATTACCCGGATTGCTCGCTGCATGAGGTCGTCATTGAATCAGATGCCGCTGAAATTGTGAGGTTGATTAACGGAGTTTCCAAAGATTGCTCAGAGATCTCCCTCCTGATCAACGAGATTCGCGAGACAGCGAATCAAATGATCTCGGTTTCGTTCGTGCATTGCCCACGGGCTTCGAACTTTTTGGCCATTCTCTAG

mRNA sequence

ATGCCGAAGAGAAATCTAGCGAAAGAGGACGAGATCATTTGGAGGAAGGATCCAAAGGGGAAGTTCTCTGTTAAGAGCGCCTATAACTTAGCTATCCAAATTGAGGCTCAAGGAATAAAAAAAAAAGATGTGGAACATGTGTTCTGGAACTACAAAGTGGTAAGATCTTTGTGGAAGATTTTATTCCCTAAACTTAATATATTATTCCACAATTGCAAGAGTTGGTGGAAGTTCAAAGACTTCTGGGATGGAGCCTCGCGAATTCTGGATGCTAAAGACGCAAGTGCGGCTAGCCACTTAATTTGGGAAATTTGGCAGAAGAGGAACAATTTGAAGCAGTCAAAAGGCAACAAAGACACAAAGCAAATTTTGCAAGACATTCTCAGTGCAGCACACAGATTCATGATCGAAAACGAGTGGGAGAAGACTAACCATGGCAAAAGGTCAGAGACCCTCCCGAGTCACGGGCGTTGGATCCCGCCGGGCAGTTCGCAATGGAAGCTGAATGTGGACGTAGCCTGGTTTGAAGCCTCAAACTCAAGCGGAGTGGGGTGGATAGTCCGGGACTCCGAAGGTTCTCTGATAGGAGCGGGCTCCAAGAAGACAAGTGGAAGATTAAAGATAAAGATGCTTGAAGCCATGACCGTGGTCGAAGGTTTCTCTATTGTATCGGAAAGATTCAGAAATTACCCGGATTGCTCGCTGCATGAGGTCGTCATTGAATCAGATGCCGCTGAAATTGTGAGGTTGATTAACGGAGTTTCCAAAGATTGCTCAGAGATCTCCCTCCTGATCAACGAGATTCGCGAGACAGCGAATCAAATGATCTCGGTTTCGTTCGTGCATTGCCCACGGGCTTCGAACTTTTTGGCCATTCTCTAG

Coding sequence (CDS)

ATGCCGAAGAGAAATCTAGCGAAAGAGGACGAGATCATTTGGAGGAAGGATCCAAAGGGGAAGTTCTCTGTTAAGAGCGCCTATAACTTAGCTATCCAAATTGAGGCTCAAGGAATAAAAAAAAAAGATGTGGAACATGTGTTCTGGAACTACAAAGTGGTAAGATCTTTGTGGAAGATTTTATTCCCTAAACTTAATATATTATTCCACAATTGCAAGAGTTGGTGGAAGTTCAAAGACTTCTGGGATGGAGCCTCGCGAATTCTGGATGCTAAAGACGCAAGTGCGGCTAGCCACTTAATTTGGGAAATTTGGCAGAAGAGGAACAATTTGAAGCAGTCAAAAGGCAACAAAGACACAAAGCAAATTTTGCAAGACATTCTCAGTGCAGCACACAGATTCATGATCGAAAACGAGTGGGAGAAGACTAACCATGGCAAAAGGTCAGAGACCCTCCCGAGTCACGGGCGTTGGATCCCGCCGGGCAGTTCGCAATGGAAGCTGAATGTGGACGTAGCCTGGTTTGAAGCCTCAAACTCAAGCGGAGTGGGGTGGATAGTCCGGGACTCCGAAGGTTCTCTGATAGGAGCGGGCTCCAAGAAGACAAGTGGAAGATTAAAGATAAAGATGCTTGAAGCCATGACCGTGGTCGAAGGTTTCTCTATTGTATCGGAAAGATTCAGAAATTACCCGGATTGCTCGCTGCATGAGGTCGTCATTGAATCAGATGCCGCTGAAATTGTGAGGTTGATTAACGGAGTTTCCAAAGATTGCTCAGAGATCTCCCTCCTGATCAACGAGATTCGCGAGACAGCGAATCAAATGATCTCGGTTTCGTTCGTGCATTGCCCACGGGCTTCGAACTTTTTGGCCATTCTCTAG

Protein sequence

MPKRNLAKEDEIIWRKDPKGKFSVKSAYNLAIQIEAQGIKKKDVEHVFWNYKVVRSLWKILFPKLNILFHNCKSWWKFKDFWDGASRILDAKDASAASHLIWEIWQKRNNLKQSKGNKDTKQILQDILSAAHRFMIENEWEKTNHGKRSETLPSHGRWIPPGSSQWKLNVDVAWFEASNSSGVGWIVRDSEGSLIGAGSKKTSGRLKIKMLEAMTVVEGFSIVSERFRNYPDCSLHEVVIESDAAEIVRLINGVSKDCSEISLLINEIRETANQMISVSFVHCPRASNFLAIL
Homology
BLAST of Spg030114 vs. NCBI nr
Match: XP_022154990.1 (uncharacterized protein LOC111022134 isoform X1 [Momordica charantia])

HSP 1 Score: 102.8 bits (255), Expect = 5.0e-18
Identity = 57/221 (25.79%), Postives = 104/221 (47.06%), Query Frame = 0

Query: 40  KKKDVEHVFWNYKVVRSLWKILFPKLNILFHNCKSWWKFKDFWDGASRILDAKDASAASH 99
           K++  +H+ W  KV++ +W    P     F+  ++ W  K++W+        ++   +  
Sbjct: 133 KEETTQHILWECKVIKDIWINCTPIEANFFYIDRTNWTTKEYWEWLMDKAGEEERRRSMI 192

Query: 100 LIWEIWQKRNNLKQSKGNKDTKQILQDILSAAHRFMIENEWEKTNHGKRSETL------- 159
           +  +IW+ RN       + +T+    DI  A  R++I +  + TN  ++S+         
Sbjct: 193 IACQIWEMRNKSIFKGVHSETR----DIQLAIDRYIINSAGQDTNLKRKSKDFHPIRRIG 252

Query: 160 -PSHGRWIPPGSSQWKLNVDVAWFEASNSSGVGWIVRDSEGSLIGAGSKKTSGRLKIKML 219
             +  RW PP S+ WKLN D AW   +N+ G+GWI+RD +G +I  G +       I  L
Sbjct: 253 DNTRARWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYL 312

Query: 220 EAMTVVEGFSIVSERFRNYPDCSLHEVVIESDAAEIVRLIN 253
           E M + EG   + +            + +ESD+ E + L++
Sbjct: 313 EVMAICEGLRAIRQE-------HCRPIHLESDSLEAIHLLH 342

BLAST of Spg030114 vs. NCBI nr
Match: XP_024046732.1 (uncharacterized protein LOC112101057 [Citrus clementina])

HSP 1 Score: 97.8 bits (242), Expect = 1.6e-16
Identity = 78/312 (25.00%), Postives = 125/312 (40.06%), Query Frame = 0

Query: 1   MPKRNLAKEDEIIWRKDPKGKFSVKSAYNLAIQIEAQGIKKKDVEHVFWNYKVVRSLWKI 60
           +P     K DE +W  D KG ++VKS Y +A++++         E +F    +   + ++
Sbjct: 56  IPLPRYPKNDERVWHYDKKGFYTVKSGYQVALKLKYVEAPTSSTESLFRRKIMQEPICQV 115

Query: 61  LFPKLNILFH---NCK---SWWKFKDFWDGASRILDAKDASAASHLI------------- 120
               +   FH    CK     WK+         ++     S    LI             
Sbjct: 116 CKSGVEDAFHAFMECKVARKVWKYSYLASEMQEVVREGILSVMQSLIKKMSMREVEFVAV 175

Query: 121 --WEIWQKRNNLKQSKGNKDTKQILQDILSAAHRFMIENEWEKTNHGKRSETLPSHGRWI 180
             WEIW  RN L       +   ++    +A   +   +  E+T+  KRSE +    +W 
Sbjct: 176 VWWEIWHARNKLIFEGKRVNPMSLIAKAQAAVEAYQRVHGKEQTS--KRSEDVSKQDQWC 235

Query: 181 PPGSSQWKLNVDVAWFEASNSSGVGWIVRDSEGSLIGAGSKKTSGRLKIKMLEAMTVVEG 240
           PP    +K+NVD A       +G+G ++R+ +G +I    K T  +  +   EA  V  G
Sbjct: 236 PPPVGFYKVNVDAAVHTEQQLTGLGVVIRNPQGQVIVTAVKSTKFQDNVTAAEAEAVKWG 295

Query: 241 FSIVSERFRNYPDCSLHEVVIESDAAEIVRLINGVSKDCSEISLLINEIRETANQMISVS 292
             I  E         L  V+IE+D  E+  L N  +    EI   I++I     +  S+S
Sbjct: 296 LEIALE-------ARLAAVIIETDCIEVANLANSKTSSRKEIVWTISDIHSYKEKFQSLS 355

BLAST of Spg030114 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 97.8 bits (242), Expect = 1.6e-16
Identity = 63/203 (31.03%), Postives = 95/203 (46.80%), Query Frame = 0

Query: 100 LIWEIWQKRNNLKQSKGNKDTKQILQDILSAAHRFMIENEWEKTNHGKRSETLPSH---- 159
           + W+IW+ RN       + +T+    DI     R++I +    TN   +S     H    
Sbjct: 15  IAWQIWEMRNKSIFKGVHSETR----DIQLVIDRYIINSAGRDTNLKGKSANKDLHLIRR 74

Query: 160 ------GRWIPPGSSQWKLNVDVAWFEASNSSGVGWIVRDSEGSLIGAGSKKTSGRLKIK 219
                  RW PP S+ WKLN D AW   +N+ G+GWI+RD +G +I A  +       I 
Sbjct: 75  IGDNTGARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNIT 134

Query: 220 MLEAMTVVEGF-SIVSERFRNYPDCSLHEVVIESDAAEIVRLINGVSKDCSEISLLINEI 279
            LE M + EG  +I  E  R         + +ESD+ E + L++   +D +EI  L+ EI
Sbjct: 135 YLEVMAICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEI 194

Query: 280 RETANQMISVSFVHCPRASNFLA 292
            +    M  VS  H  R +N +A
Sbjct: 195 WQMMEDMKIVSMRHISREANKVA 213

BLAST of Spg030114 vs. NCBI nr
Match: CAB4262994.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 97.1 bits (240), Expect = 2.8e-16
Identity = 76/306 (24.84%), Postives = 133/306 (43.46%), Query Frame = 0

Query: 1    MPKRNLAKEDEIIWRKDPKGKFSVKSAYNLAIQIEAQGIKKKDVEHVFWNYKVVRSLWKI 60
            +P  +LA  D +IW  +  G +SVKS Y L  ++E   +  +    V  + +  + +W +
Sbjct: 1383 IPLVSLASHDCLIWHYERNGMYSVKSGYQLT-RLEKDIMCGESSAGVNLSSRFWKKIWAL 1442

Query: 61   LFP-KLN-ILFHNCKSWW------------KFKDFWDGASRILDAKDASAASHLIWEIWQ 120
              P K+   L+  C +               F++ W         ++    ++L W +W 
Sbjct: 1443 KIPNKIKFFLWRRCGATQLGGNVCEVSRVNSFRELWYAVQISSGGEEQGLFAYLRWGLWN 1502

Query: 121  KRNNLKQSKGNKDTKQILQDILSAAHRFMIENEWEKTNHGKRSETLPSHGRWIPPGSSQW 180
             R++L     ++   Q+L  + + A  F   N    T+HG++S    S   W PP    +
Sbjct: 1503 WRDSLIFEAKSETATQLLHRMHTLAQEFFDVNNSSHTSHGRQSSPQASLHVWRPPAVGIY 1562

Query: 181  KLNVDVAWFEASNSSGVGWIVRDSEGSLIGAGSKKTSGRLKIKMLEAMTVVEGFSIVSER 240
            K+NVD A     +  GVG +VR+  G  + A  ++       + +E M  +EG       
Sbjct: 1563 KINVDRALKSGDSIRGVGVVVRNENGDFMAACVRRIQASYGARQMELMATIEG------- 1622

Query: 241  FRNYPDCSLHEVVIESDAAEIVRLINGVSKDCSEI-SLLINEIRETANQMISVSFVHCPR 292
             R   D    + V+E DA + +  I  +S +C+ +  LLI E++   N   +V     PR
Sbjct: 1623 LRFAIDMGFTDAVLEMDAQDCIHSI--LSTECNGVDGLLIEEVKSLLNNFRAVGCQWTPR 1678

BLAST of Spg030114 vs. NCBI nr
Match: EEC72753.1 (hypothetical protein OsI_06384 [Oryza sativa Indica Group])

HSP 1 Score: 96.3 bits (238), Expect = 4.7e-16
Identity = 69/255 (27.06%), Postives = 116/255 (45.49%), Query Frame = 0

Query: 44  VEHVFWNYKVVRSLWKILFPKLNIL-----FHNCKSWWKFKDFWDGASRILDAKDASAAS 103
           +EHVF       S+WK +    NI        N + W    DF    S I +    +  +
Sbjct: 288 IEHVFLMCPFSDSVWKEIKQVYNIKLGRGDLANVRQW--IFDFLRRGSAIQN----TVLA 347

Query: 104 HLIWEIWQKRNNLKQSKGNKDTKQILQDILSAAHRFMIENEWEKTNHGKRSE--TLPSHG 163
              W IW+ RNN K +      ++++Q I+  A+  MI   +   N  + +E  +LPS  
Sbjct: 348 VTFWHIWEARNNAKNNVETLHPRRVVQKIV--AYVEMIMQHYGPGNVSRPAERISLPS-- 407

Query: 164 RWIPPGSSQWKLNVDVAWFEASNSSGVGWIVRDSEGSLIGAGSKKTSGRLKIKMLEAMTV 223
            W+PP +  + +N D A F+A    GVG ++RD  G+ + A + +  G    +  EA  +
Sbjct: 408 -WVPPPAGVFLINTDAAVFQAERQMGVGVVIRDQHGNCLLAANTRFMGITDPETAEACAI 467

Query: 224 VEGFSIVSERFRNYPDCSLHEVVIESDAAEIVRLINGVSKDCSEISLLINEIRETANQMI 283
                +  E          H V++  D   +++ +N   +D S+I  L+ +I++   + I
Sbjct: 468 RHALWLAKEE-------GFHNVIVACDCLSVIQKLNSPGRDRSKIGCLVEDIKKLGVEFI 524

Query: 284 SVSFVHCPRASNFLA 292
           SVSF+H    SN  A
Sbjct: 528 SVSFIHVSHCSNVAA 524

BLAST of Spg030114 vs. ExPASy TrEMBL
Match: A0A6J1DL64 (uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 2.4e-18
Identity = 57/221 (25.79%), Postives = 104/221 (47.06%), Query Frame = 0

Query: 40  KKKDVEHVFWNYKVVRSLWKILFPKLNILFHNCKSWWKFKDFWDGASRILDAKDASAASH 99
           K++  +H+ W  KV++ +W    P     F+  ++ W  K++W+        ++   +  
Sbjct: 133 KEETTQHILWECKVIKDIWINCTPIEANFFYIDRTNWTTKEYWEWLMDKAGEEERRRSMI 192

Query: 100 LIWEIWQKRNNLKQSKGNKDTKQILQDILSAAHRFMIENEWEKTNHGKRSETL------- 159
           +  +IW+ RN       + +T+    DI  A  R++I +  + TN  ++S+         
Sbjct: 193 IACQIWEMRNKSIFKGVHSETR----DIQLAIDRYIINSAGQDTNLKRKSKDFHPIRRIG 252

Query: 160 -PSHGRWIPPGSSQWKLNVDVAWFEASNSSGVGWIVRDSEGSLIGAGSKKTSGRLKIKML 219
             +  RW PP S+ WKLN D AW   +N+ G+GWI+RD +G +I  G +       I  L
Sbjct: 253 DNTRARWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYL 312

Query: 220 EAMTVVEGFSIVSERFRNYPDCSLHEVVIESDAAEIVRLIN 253
           E M + EG   + +            + +ESD+ E + L++
Sbjct: 313 EVMAICEGLRAIRQE-------HCRPIHLESDSLEAIHLLH 342

BLAST of Spg030114 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 7.8e-17
Identity = 63/203 (31.03%), Postives = 95/203 (46.80%), Query Frame = 0

Query: 100 LIWEIWQKRNNLKQSKGNKDTKQILQDILSAAHRFMIENEWEKTNHGKRSETLPSH---- 159
           + W+IW+ RN       + +T+    DI     R++I +    TN   +S     H    
Sbjct: 15  IAWQIWEMRNKSIFKGVHSETR----DIQLVIDRYIINSAGRDTNLKGKSANKDLHLIRR 74

Query: 160 ------GRWIPPGSSQWKLNVDVAWFEASNSSGVGWIVRDSEGSLIGAGSKKTSGRLKIK 219
                  RW PP S+ WKLN D AW   +N+ G+GWI+RD +G +I A  +       I 
Sbjct: 75  IGDNTGARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNIT 134

Query: 220 MLEAMTVVEGF-SIVSERFRNYPDCSLHEVVIESDAAEIVRLINGVSKDCSEISLLINEI 279
            LE M + EG  +I  E  R         + +ESD+ E + L++   +D +EI  L+ EI
Sbjct: 135 YLEVMAICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEI 194

Query: 280 RETANQMISVSFVHCPRASNFLA 292
            +    M  VS  H  R +N +A
Sbjct: 195 WQMMEDMKIVSMRHISREANKVA 213

BLAST of Spg030114 vs. ExPASy TrEMBL
Match: A0A2N9GAE6 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS24083 PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.7e-16
Identity = 74/261 (28.35%), Postives = 118/261 (45.21%), Query Frame = 0

Query: 35   EAQGIKKKDVEHVFWNYKVVRSLWKILFPKLNILFHNCKSWWKFKDFWDGASRILD-AKD 94
            E  G K +D  H  W+ K ++S+W       N ++          DF D  S++L   +D
Sbjct: 1141 EICGEKDEDAIHALWSCKQLQSVWS------NEVWAQSLQNSPMMDFADLLSKVLQHGRD 1200

Query: 95   ASAASHLI--WEIWQKRNNLKQSKGNKDTKQILQDILSAAHRFMIENEWEKTNHGKRSET 154
            +     +I  W +WQ+RN ++  +      Q+     S    +M ENE  K      S+ 
Sbjct: 1201 SEPELFIIICWALWQRRNKIRLHQEVDSINQVGLQAKSYLEEYMSENELSK------SKP 1260

Query: 155  LPSHG-RWIPPGSSQWKLNVDVAWFEASNSSGVGWIVRDSEGSLIGAGSKKTSGRLKIKM 214
             P+   RW+PP   ++K+N D A F+ +N +G+G IVRDS G ++ + ++K      +  
Sbjct: 1261 QPALAVRWMPPRKHKYKVNYDGAVFKETNEAGIGVIVRDSFGLVMASLTQKVRFPHSVPS 1320

Query: 215  LEAMTVVEGFSIVSERFRNYPDCSLHEVVIESDAAEIVRLINGVSKDCSEISLLINEIRE 274
            +EA  V      V E         L E   E D+  IV  +N      +   LLI + + 
Sbjct: 1321 IEAWAVKRSIQFVLE-------IGLTEAEFEGDSQTIVAALNDPKPSLAPFGLLITDAKV 1380

Query: 275  TANQMISVSFVHCPRASNFLA 292
             A+++ S SF H  R  N LA
Sbjct: 1381 LASKLQSYSFSHVKRQGNQLA 1382

BLAST of Spg030114 vs. ExPASy TrEMBL
Match: B8AE80 (Uncharacterized protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_06384 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 2.3e-16
Identity = 69/255 (27.06%), Postives = 116/255 (45.49%), Query Frame = 0

Query: 44  VEHVFWNYKVVRSLWKILFPKLNIL-----FHNCKSWWKFKDFWDGASRILDAKDASAAS 103
           +EHVF       S+WK +    NI        N + W    DF    S I +    +  +
Sbjct: 288 IEHVFLMCPFSDSVWKEIKQVYNIKLGRGDLANVRQW--IFDFLRRGSAIQN----TVLA 347

Query: 104 HLIWEIWQKRNNLKQSKGNKDTKQILQDILSAAHRFMIENEWEKTNHGKRSE--TLPSHG 163
              W IW+ RNN K +      ++++Q I+  A+  MI   +   N  + +E  +LPS  
Sbjct: 348 VTFWHIWEARNNAKNNVETLHPRRVVQKIV--AYVEMIMQHYGPGNVSRPAERISLPS-- 407

Query: 164 RWIPPGSSQWKLNVDVAWFEASNSSGVGWIVRDSEGSLIGAGSKKTSGRLKIKMLEAMTV 223
            W+PP +  + +N D A F+A    GVG ++RD  G+ + A + +  G    +  EA  +
Sbjct: 408 -WVPPPAGVFLINTDAAVFQAERQMGVGVVIRDQHGNCLLAANTRFMGITDPETAEACAI 467

Query: 224 VEGFSIVSERFRNYPDCSLHEVVIESDAAEIVRLINGVSKDCSEISLLINEIRETANQMI 283
                +  E          H V++  D   +++ +N   +D S+I  L+ +I++   + I
Sbjct: 468 RHALWLAKEE-------GFHNVIVACDCLSVIQKLNSPGRDRSKIGCLVEDIKKLGVEFI 524

Query: 284 SVSFVHCPRASNFLA 292
           SVSF+H    SN  A
Sbjct: 528 SVSFIHVSHCSNVAA 524

BLAST of Spg030114 vs. ExPASy TrEMBL
Match: A0A7N2N157 (RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 5.1e-16
Identity = 67/250 (26.80%), Postives = 110/250 (44.00%), Query Frame = 0

Query: 42  KDVEHVFWNYKVVRSLWKILFPKLNILFHNCKSWWKFKDFWDGASRILDAKDASAASHLI 101
           +D  H  W  +V++ +W       N L      +  F+D W G       K A   +++ 
Sbjct: 29  EDTIHALWGCQVLKEVWWNEHYLRNQL---STRYVDFRDLWIGIINSEVPKLAERFAYVA 88

Query: 102 WEIWQKRNNLKQSKGNKDTKQILQDILSAAHRFMIENEWEKTNHGKRSETLPSHGRWIPP 161
           W IW KRN  +    +    +I QD+      F    E       ++    P+H  W PP
Sbjct: 89  WSIWHKRNATRMQSSSLPFNRIYQDMCDRLQEFQAARELAIP---EQIPAGPTH--WSPP 148

Query: 162 GSSQWKLNVDVAWFEASNSSGVGWIVRDSEGSLIGAGSKKTSGRLKIKMLEAMTVVEGFS 221
             +Q K+N D A F  +  +G+G +VR+S G++ GA S +    + +  +EA+       
Sbjct: 149 QRTQSKVNYDGALFHDTKQAGIGVVVRNSGGTIQGALSDRCCLPMTVDEVEALACRSAVL 208

Query: 222 IVSERFRNYPDCSLHEVVIESDAAEIVRLINGVSKDCSEISLLINEIRETANQMISVSFV 281
            V +         L EVV E D+  I + +N  S   S    +I++++  A   +SVSF+
Sbjct: 209 YVIQ-------LGLEEVVFEGDSETITKALNSDSPCLSSFGHIIDDVKSLALNFVSVSFI 263

Query: 282 HCPRASNFLA 292
           H  R  N +A
Sbjct: 269 HVKRQGNVVA 263

BLAST of Spg030114 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 69.3 bits (168), Expect = 5.8e-12
Identity = 42/158 (26.58%), Postives = 75/158 (47.47%), Query Frame = 0

Query: 100 LIWEIWQKRNNLKQSKGNKDTKQILQDILSAAHRFMIENEWEKTNHGKRSETLPSHGRWI 159
           L+W IW+  N+L  +      +  ++  L+    ++      +  +G R+     + +W 
Sbjct: 47  LMWRIWKSGNDLVFNHTRTKFQTTVEMALNDTKEWLDNTMTNEQQNGNRNADPSRNTKWS 106

Query: 160 PPGSSQWKLNVDVAWFEASNSSGVGWIVRDSEGSLIGAGSKKTSGRLKIKMLEAMTVVEG 219
           PPG  + K N D +  E +  SG+GWI+R+S+G++I  G  K  GR+  +  E  T++  
Sbjct: 107 PPGRDKLKCNYDASHHERNTVSGLGWILRNSQGTVIECGMGKFQGRMTTEEAECSTLIWA 166

Query: 220 FSIVSERFRNYPDCSLHEVVIESDAAEIVRLINGVSKD 258
               S  F +       +V+ E D   I R+IN  S +
Sbjct: 167 IQ-ASYGFGH------KKVIFEGDNQTITRMINTKSSN 197

BLAST of Spg030114 vs. TAIR 10
Match: AT1G52990.1 (thioredoxin family protein )

HSP 1 Score: 46.2 bits (108), Expect = 5.2e-05
Identity = 31/126 (24.60%), Postives = 54/126 (42.86%), Query Frame = 0

Query: 163 SSQWKLNVDVAWFEASNSSGVGWIVRDSEGSLIGAGSKKTSGRLKIKMLEAMTVVEGFSI 222
           S + K N D +  E    SG+GW++R+S+G+++  G  K  GR+  +  E   ++     
Sbjct: 45  SCRVKCNYDASHHEGDVVSGLGWLIRNSQGTVLECGMGKFQGRMTPEEAECSALIWAIQA 104

Query: 223 VSERFRNYPDCSLHEVVIESDAAEIVRLINGVSKDCSEISLLINEIRETANQMISVSFVH 282
            S            +V+ E D + + RLIN    D   +   ++ I+       S  F+ 
Sbjct: 105 TSA-------FGYTKVIFEGDNSNVNRLIN-TKSDNPRLKHYLDTIKSWIPSFTSTEFIF 162

Query: 283 CPRASN 289
             R  N
Sbjct: 165 THREQN 162

BLAST of Spg030114 vs. TAIR 10
Match: AT3G23320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 46.2 bits (108), Expect = 5.2e-05
Identity = 40/160 (25.00%), Postives = 60/160 (37.50%), Query Frame = 0

Query: 135 MIENEWEKTNHG-----KRSETLPSH-GRWIPPGSSQWKLNVDVAWFEASNSSGVGWIVR 194
           M   EW    H      K  +T  S   +W  PG+   K N DV+       SG+ WI+R
Sbjct: 1   MDTKEWLDVKHKNDDNVKGGQTKSSRTDKWQKPGAEWVKCNYDVSNHAGRQDSGLRWIIR 60

Query: 195 DSEGSLIGAGSKKTSGRLKIKMLEAMTVVEGFSIVSERFRNYPDCSLHEVVIESDAAEIV 254
           +S+G+ +  G  K  GR  IK  E   ++              D     V  E D   + 
Sbjct: 61  NSQGTCLDCGCGKFQGRQTIKEAECTALIWAIQCAW-------DLGYRRVEFEGDNITVN 120

Query: 255 RLINGVSKDCSEISLLINEIRETANQMISVSFVHCPRASN 289
           RLI     +   +   +  I++ +    +V F    R  N
Sbjct: 121 RLIRNKETN-PRLRYYLEFIQQWSKAFTTVKFTFRHREQN 152

BLAST of Spg030114 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 45.4 bits (106), Expect = 8.9e-05
Identity = 45/203 (22.17%), Postives = 82/203 (40.39%), Query Frame = 0

Query: 100 LIWEIWQKRNNLKQSKGNKDTKQILQDILSAAHRFMIENEWEKTN----------HGKR- 159
           ++W +W+ RN L   + +   +  LQ   +         EWE TN          H  R 
Sbjct: 123 ILWRLWKSRNQLVFQQKSISWQNTLQRARNDV------QEWEDTNTYVQSLNQQVHSSRH 182

Query: 160 SETLPSHGRWIPPGSSQWKLNVDVAWFEASNSSGVGWIVRDSEGSLIGAG---SKKTSGR 219
            +   +  +W  P S+  K N D A+   + ++  GW++RD  G  +G+G      TS  
Sbjct: 183 QQPTMARTKWQRPPSTWIKYNYDGAFNHQTRNAKAGWLMRDENGVYMGSGQAIGSTTSDS 242

Query: 220 LKIKMLEAMTVVEGFSIVSERFRNYPDCSLHEVVIESDAAEIVRLINGVSKDCSEISLLI 279
           L+ +    +  ++     S+ +R        +V+ E D+ ++  L+N    +    +  I
Sbjct: 243 LESEFQALIIAMQ--HAWSQGYR--------KVIFEGDSKQVEELMNNEKLNFGRFN-WI 302

Query: 280 NEIRETANQMISVSFVHCPRASN 289
            E R    +     F   PR +N
Sbjct: 303 REGRFWQKRFEEAVFKWVPRTNN 308

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154990.15.0e-1825.79uncharacterized protein LOC111022134 isoform X1 [Momordica charantia][more]
XP_024046732.11.6e-1625.00uncharacterized protein LOC112101057 [Citrus clementina][more]
XP_022156777.11.6e-1631.03uncharacterized protein LOC111023608 [Momordica charantia][more]
CAB4262994.12.8e-1624.84unnamed protein product [Prunus armeniaca][more]
EEC72753.14.7e-1627.06hypothetical protein OsI_06384 [Oryza sativa Indica Group][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DL642.4e-1825.79uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1DSV17.8e-1731.03uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A2N9GAE61.7e-1628.35Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS24083 PE=4 SV=1[more]
B8AE802.3e-1627.06Uncharacterized protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_06384 PE=4... [more]
A0A7N2N1575.1e-1626.80RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G65005.15.8e-1226.58Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G52990.15.2e-0524.60thioredoxin family protein [more]
AT3G23320.15.2e-0525.00Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT3G25270.18.9e-0522.17Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 169..291
e-value: 7.0E-17
score: 61.4
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 163..292
e-value: 2.5E-9
score: 39.3
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 153..291
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 168..291
e-value: 1.97393E-16
score: 71.9616

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg030114.1Spg030114.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity