Spg030032 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg030032
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionbasic helix-loop-helix (bHLH) DNA-binding superfamily protein
Locationscaffold6: 12171701 .. 12173119 (+)
RNA-Seq ExpressionSpg030032
SyntenySpg030032
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGCCAAGATTCAGCTCTGTTATTCCATCAGTATCAATTCAACAACAGCAACACCACGTGCGATTCACATGCATTCGACGAGTGGCGGGCTTGCGGCGGCCGGATAACGTCCATACCGGCGGCGGCGGCGGCGGCGTCTCAGATTATTTTGTTTGAGGAGGGCAAGTTTTGTCCGGCGGGTTTGGTGGAGCCAAAGGCTGAGATTTGTGGCTATGGGAACGGGGATTTTTGGCCGTTGATGAATGTCGCTGAAATGAGAAAGAAGAGATTGATGAGCAAAAATCCGCCGCGTGCTGACGATCACGTTCTGGCTGAGAGGAAACGCCGGGAGAAACTCAGCCAGAGATTCATCGCTCTTTCTGCTGTCTTACCTGGCCTTAAGAAGGTACTTCAATTAACTCATTTTAGGTTAAATTGTTTGTCTGTGTATTTTGACCTAGATTCTCAAACATACAGTGGGATTGTCTCTTTAAAACGGTCGGATGTAGATCTTAATGATTGTCTATCCCTAATAACTGTTGGATATAAAATCAATATACTCTTACCATTTCTCTCTTACCTCTAACGATACAAAACTATCGACCTTATTTTGACGGCAACATAGATAATAACTTCTTAGGCATAAGATTATTTAATTAAAAGGAAGACACTAATAAGTGATATAACATAGATTGTTCTATTGTATCCTTTTTCATGCTTATCTTTCTTTTTAACAACACTTTACATACTAGGGTTCAAAGTAATAAATTAAAGCTTGAGATGAAGATTTTTAGGCGAGGATTTGTTTTGGATCTCACCTCAAAAAGATAAGTGGAGATCTTACAGTTTATAAGATACATAGTTACTTCACTCATAGTCAATTGATTTTGAGATGAAACCTCATATTTATCTAATATGGTATCAGATTTTGAGATGAAACTCTCTAATAGAATTTTCATTGACGGGTTAAAAATTGAGATGAGATGGTAATTTTCTTTTTGAAACCAAACAGATGGACAAAGCCTCAGTTCTTGGAGATGCAATCGAGTACATAAAACAACTAAAAGAACGAGTGGAATTGCTGGAGGAGAAGGCCAAAAATAAGAGCATCGAATCTGGCATTTTGATAAAGAAATCCCATTCATATGAATATGATTGTGGAGAGGATGATGATCAGAAGCAGGTTACTAGATTTTCCGACCAAAAGCTACCGGAAATCGAAGTGAGAATCTCAGACAAACATGTTCTTGTTCGAATCCACTCTGAGAAACATGAGGGTTTGTTCGCAAAGTTATTAGGGGAAATGGAGAAGCTCAATTTTACCATCGTTAATGGCAATGTTTTCCCCTTTGGCACTTCAATGCAATATATGGTTTTTGTTGCTCAGGTATGTTTCCATTTATTTATTAGCTCCTGTAACGATTTTGGTTTTTGA

mRNA sequence

ATGGAAGGCCAAGATTCAGCTCTGTTATTCCATCAGTATCAATTCAACAACAGCAACACCACGTGCGATTCACATGCATTCGACGAGTGGCGGGCTTGCGGCGGCCGGATAACGTCCATACCGGCGGCGGCGGCGGCGGCGTCTCAGATTATTTTGTTTGAGGAGGGCAAGTTTTGTCCGGCGGGTTTGGTGGAGCCAAAGGCTGAGATTTGTGGCTATGGGAACGGGGATTTTTGGCCGTTGATGAATGTCGCTGAAATGAGAAAGAAGAGATTGATGAGCAAAAATCCGCCGCGTGCTGACGATCACGTTCTGGCTGAGAGGAAACGCCGGGAGAAACTCAGCCAGAGATTCATCGCTCTTTCTGCTGTCTTACCTGGCCTTAAGAAGATGGACAAAGCCTCAGTTCTTGGAGATGCAATCGAGTACATAAAACAACTAAAAGAACGAGTGGAATTGCTGGAGGAGAAGGCCAAAAATAAGAGCATCGAATCTGGCATTTTGATAAAGAAATCCCATTCATATGAATATGATTGTGGAGAGGATGATGATCAGAAGCAGGTTACTAGATTTTCCGACCAAAAGCTACCGGAAATCGAAGTGAGAATCTCAGACAAACATGTTCTTGTTCGAATCCACTCTGAGAAACATGAGGGTTTGTTCGCAAAGTTATTAGGGGAAATGGAGAAGCTCAATTTTACCATCGTTAATGGCAATGTTTTCCCCTTTGGCACTTCAATGCAATATATGGTTTTTGTTGCTCAGGTATGTTTCCATTTATTTATTAGCTCCTGTAACGATTTTGGTTTTTGA

Coding sequence (CDS)

ATGGAAGGCCAAGATTCAGCTCTGTTATTCCATCAGTATCAATTCAACAACAGCAACACCACGTGCGATTCACATGCATTCGACGAGTGGCGGGCTTGCGGCGGCCGGATAACGTCCATACCGGCGGCGGCGGCGGCGGCGTCTCAGATTATTTTGTTTGAGGAGGGCAAGTTTTGTCCGGCGGGTTTGGTGGAGCCAAAGGCTGAGATTTGTGGCTATGGGAACGGGGATTTTTGGCCGTTGATGAATGTCGCTGAAATGAGAAAGAAGAGATTGATGAGCAAAAATCCGCCGCGTGCTGACGATCACGTTCTGGCTGAGAGGAAACGCCGGGAGAAACTCAGCCAGAGATTCATCGCTCTTTCTGCTGTCTTACCTGGCCTTAAGAAGATGGACAAAGCCTCAGTTCTTGGAGATGCAATCGAGTACATAAAACAACTAAAAGAACGAGTGGAATTGCTGGAGGAGAAGGCCAAAAATAAGAGCATCGAATCTGGCATTTTGATAAAGAAATCCCATTCATATGAATATGATTGTGGAGAGGATGATGATCAGAAGCAGGTTACTAGATTTTCCGACCAAAAGCTACCGGAAATCGAAGTGAGAATCTCAGACAAACATGTTCTTGTTCGAATCCACTCTGAGAAACATGAGGGTTTGTTCGCAAAGTTATTAGGGGAAATGGAGAAGCTCAATTTTACCATCGTTAATGGCAATGTTTTCCCCTTTGGCACTTCAATGCAATATATGGTTTTTGTTGCTCAGGTATGTTTCCATTTATTTATTAGCTCCTGTAACGATTTTGGTTTTTGA

Protein sequence

MEGQDSALLFHQYQFNNSNTTCDSHAFDEWRACGGRITSIPAAAAAASQIILFEEGKFCPAGLVEPKAEICGYGNGDFWPLMNVAEMRKKRLMSKNPPRADDHVLAERKRREKLSQRFIALSAVLPGLKKMDKASVLGDAIEYIKQLKERVELLEEKAKNKSIESGILIKKSHSYEYDCGEDDDQKQVTRFSDQKLPEIEVRISDKHVLVRIHSEKHEGLFAKLLGEMEKLNFTIVNGNVFPFGTSMQYMVFVAQVCFHLFISSCNDFGF
Homology
BLAST of Spg030032 vs. NCBI nr
Match: XP_022985138.1 (transcription factor bHLH25-like [Cucurbita maxima])

HSP 1 Score: 281.6 bits (719), Expect = 7.3e-72
Identity = 169/269 (62.83%), Postives = 194/269 (72.12%), Query Frame = 0

Query: 2   EGQDSALLFH-QYQFNNSNTTCDS-HAFDEWRACGGRITSIPAAAAAASQIILFEEGKFC 61
           E QDS+LLFH QYQ  N+  T DS   F+ WR+ GG      A A+++SQ+ILF+EGKFC
Sbjct: 4   EEQDSSLLFHDQYQLFNATPTSDSPPLFNHWRSYGGS----AALASSSSQMILFQEGKFC 63

Query: 62  PAGLVEPKAEICGYGNGDFWPLMNVAEMRKKRLMSKNPPRADDHVLAERKRREKLSQRFI 121
           P   V   AEI        WPL+NV          +NPPRA DHVLAERKRREKLSQRFI
Sbjct: 64  P---VAAAAEI--------WPLVNV---------GRNPPRAKDHVLAERKRREKLSQRFI 123

Query: 122 ALSAVLPGLKKMDKASVLGDAIEYIKQLKERVELLEEKAKNKSIESGILIKKSH--SYEY 181
           ALSA+LPGLKKMDKAS+LGDAI+YIKQL+ERVE LEE  KN +IES ILIKKSH  +   
Sbjct: 124 ALSALLPGLKKMDKASILGDAIKYIKQLQERVESLEEMGKNSNIESAILIKKSHLCNNMN 183

Query: 182 DCGEDD--DQKQVTRFSDQKLPEIEVRISDKHVLVRIHSEKHEGLFAKLLGEMEKLNFTI 241
           DC + D  +Q   TRFSDQKL EIE +ISDKHVLVRIH EKHEGL A+LL EMEKLNF I
Sbjct: 184 DCDDIDGEEQNHKTRFSDQKLVEIEAKISDKHVLVRIHCEKHEGLLARLLEEMEKLNFRI 243

Query: 242 VNGNVFPFGTSMQYMVFVAQVCFHLFISS 265
           +N NVFPFG+S QYMVF+ Q   + FI S
Sbjct: 244 INANVFPFGSSTQYMVFLLQRNSNSFIDS 248

BLAST of Spg030032 vs. NCBI nr
Match: XP_023552894.1 (transcription factor bHLH25-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 280.8 bits (717), Expect = 1.2e-71
Identity = 164/263 (62.36%), Postives = 190/263 (72.24%), Query Frame = 0

Query: 1   MEG-QDSALLFH-QYQFNNSNTTCDS-HAFDEWRACGGRITSIPAAAAAASQIILFEEGK 60
           MEG QDS+LLFH QYQ  N+  T DS   F+ WR+CGG      A A+++SQ+ILF+EGK
Sbjct: 1   MEGEQDSSLLFHDQYQLFNATPTSDSPPLFNHWRSCGGS----AAVASSSSQMILFQEGK 60

Query: 61  FCPAGLVEPKAEICGYGNGDFWPLMNVAEMRKKRLMSKNPPRADDHVLAERKRREKLSQR 120
           FCPA               + WPL+NV          +NPPRA DHVLAERKRREKLSQR
Sbjct: 61  FCPAAAAA----------AEIWPLVNV---------GRNPPRAKDHVLAERKRREKLSQR 120

Query: 121 FIALSAVLPGLKKMDKASVLGDAIEYIKQLKERVELLEEKAKNKSIESGILIKKSH--SY 180
           FIALSA+LPGLKKMDKAS+LGDAI+YIKQL+ERVE LEE  KN +IE+ ILIKKSH  + 
Sbjct: 121 FIALSALLPGLKKMDKASILGDAIKYIKQLQERVESLEEMGKNSNIEAAILIKKSHLCNN 180

Query: 181 EYDCGED---DDQKQVTRFSDQKLPEIEVRISDKHVLVRIHSEKHEGLFAKLLGEMEKLN 240
             DC +D   ++    T FSDQKL EIE +ISDKHVLVRIH EKHEGL AKLL EMEKLN
Sbjct: 181 MNDCCDDIDGEEHNHKTGFSDQKLVEIEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLN 240

Query: 241 FTIVNGNVFPFGTSMQYMVFVAQ 256
           F I+N NVFPFG+S QYMVF+ Q
Sbjct: 241 FKIINVNVFPFGSSTQYMVFLLQ 240

BLAST of Spg030032 vs. NCBI nr
Match: XP_022929215.1 (transcription factor bHLH25-like [Cucurbita moschata])

HSP 1 Score: 278.9 bits (712), Expect = 4.7e-71
Identity = 161/261 (61.69%), Postives = 187/261 (71.65%), Query Frame = 0

Query: 2   EGQDSALLFH-QYQFNNSNTTCDS-HAFDEWRACGGRITSIPAAAAAASQIILFEEGKFC 61
           E QDS+LLFH QYQ  N+  T DS   F+ WR+CGG      A A+++SQ+ILF+EGKFC
Sbjct: 4   EEQDSSLLFHDQYQLFNATPTSDSPPLFNHWRSCGGS----AAVASSSSQMILFQEGKFC 63

Query: 62  PAGLVEPKAEICGYGNGDFWPLMNVAEMRKKRLMSKNPPRADDHVLAERKRREKLSQRFI 121
           PA               + WPL+NV          +NPPRA DHVLAERKRREKLSQRFI
Sbjct: 64  PAA-------------AEIWPLVNV---------GRNPPRAKDHVLAERKRREKLSQRFI 123

Query: 122 ALSAVLPGLKKMDKASVLGDAIEYIKQLKERVELLEEKAKNKSIESGILIKKSH--SYEY 181
           ALSA+LPGLKKMDKAS+LGDAI+YIKQL+ERVE LEE  KN +IES I IKKSH  +   
Sbjct: 124 ALSALLPGLKKMDKASILGDAIKYIKQLQERVESLEEMGKNSNIESAIFIKKSHLCNNMN 183

Query: 182 DCGED---DDQKQVTRFSDQKLPEIEVRISDKHVLVRIHSEKHEGLFAKLLGEMEKLNFT 241
            C +D   ++    T FSDQKL E+E +ISDKHVLVRIH EKHEGL AKLL EMEKLNF 
Sbjct: 184 GCCDDIDGEEHNHKTGFSDQKLVEMEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFK 238

Query: 242 IVNGNVFPFGTSMQYMVFVAQ 256
           I+NGNVFPFG+S QYMVF+ Q
Sbjct: 244 IINGNVFPFGSSTQYMVFLLQ 238

BLAST of Spg030032 vs. NCBI nr
Match: KAG7015418.1 (Transcription factor bHLH25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 231.1 bits (588), Expect = 1.1e-56
Identity = 127/186 (68.28%), Postives = 144/186 (77.42%), Query Frame = 0

Query: 75  NGDFWPLMNVAEMRKKRLMSKNPPRADDHVLAERKRREKLSQRFIALSAVLPGLKKMDKA 134
           + + WPL+NV          +NPPRA DHVLAERKRREKLSQRFIALSA+LPGLKKMDKA
Sbjct: 7   SAEIWPLVNV---------GRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKA 66

Query: 135 SVLGDAIEYIKQLKERVELLEEKAKNKSIESGILIKKSH--SYEYDCGED---DDQKQVT 194
           S+LGDAI+YIKQL+ERVE LEE  KN +IES I IKKSH  +   DC +D   ++    T
Sbjct: 67  SILGDAIKYIKQLQERVESLEEMGKNSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKT 126

Query: 195 RFSDQKLPEIEVRISDKHVLVRIHSEKHEGLFAKLLGEMEKLNFTIVNGNVFPFGTSMQY 254
            FSDQKL EIE +ISDKHVLVRIH EKHEGL AKLL EMEKLNF I+N NVFPFG+S QY
Sbjct: 127 GFSDQKLVEIEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQY 183

Query: 255 MVFVAQ 256
           MVF+ Q
Sbjct: 187 MVFLLQ 183

BLAST of Spg030032 vs. NCBI nr
Match: XP_022136753.1 (transcription factor bHLH18-like isoform X3 [Momordica charantia])

HSP 1 Score: 225.7 bits (574), Expect = 4.7e-55
Identity = 145/246 (58.94%), Postives = 163/246 (66.26%), Query Frame = 0

Query: 17  NSNTTCDSHAFDEWRACGGRITSIPAAAAAASQIILFEEGKFCPAGLVEPKAEICGYGNG 76
           N+N TCDS AF                  AAS     EE  F       P AEI    +G
Sbjct: 2   NNNNTCDSRAF-----------------VAAS-----EEPTFS-----RPTAEIICSSHG 61

Query: 77  ---DFWPLMNVAEMRKKRLMSKNPPRADDHVLAERKRREKLSQRFIALSAVLPGLKKMDK 136
               F P      + +KR  +     A DHVLAERKRREKLSQRFIALSA+LPGLKKMDK
Sbjct: 62  MHTSFSPPSINNNIPEKRKPTPTTAHAQDHVLAERKRREKLSQRFIALSALLPGLKKMDK 121

Query: 137 ASVLGDAIEYIKQLKERVELLEEKAKNKSIESGILIKKSHSYEYDCGEDDDQKQVTRFSD 196
           ASVLGDAI+YIKQ++ERV +LEEKAK +SIES IL+ KSH Y  DC E     +++RFSD
Sbjct: 122 ASVLGDAIKYIKQVEERVRVLEEKAKKRSIESAILVNKSHLYN-DCDE-----EISRFSD 181

Query: 197 QK-LPEIEVRISD--KHVLVRIHSEKHEGLFAKLLGEMEKLNFTIVNGNVFPFGTSMQYM 256
           QK LPEIE RISD   +VLVRIH EK+EGLFAKLLGEMEK+N TIVN N FPFGTS QYM
Sbjct: 182 QKQLPEIEARISDDKNNVLVRIHCEKNEGLFAKLLGEMEKMNLTIVNANFFPFGTSTQYM 214

BLAST of Spg030032 vs. ExPASy Swiss-Prot
Match: Q9T072 (Transcription factor bHLH25 OS=Arabidopsis thaliana OX=3702 GN=BHLH25 PE=2 SV=2)

HSP 1 Score: 151.8 bits (382), Expect = 1.1e-35
Identity = 82/164 (50.00%), Postives = 114/164 (69.51%), Query Frame = 0

Query: 88  RKKRLMSKNPPRADDHVLAERKRREKLSQRFIALSAVLPGLKKMDKASVLGDAIEYIKQL 147
           ++ +  S+N   A DH++AERKRREKL+QRF+ALSA++PGLKKMDKASVLGDA+++IK L
Sbjct: 138 KRAQPFSRNQSNAQDHIIAERKRREKLTQRFVALSALVPGLKKMDKASVLGDALKHIKYL 197

Query: 148 KERVELLEEKAKNKSIESGILIKKSHSYEYDCGEDDDQKQVTRFSDQKLPEIEVRISDKH 207
           +ERV  LEE+ K + +ES +L+KKS     D  +         FSD  LPEIEVR SD+ 
Sbjct: 198 QERVGELEEQKKERRLESMVLVKKSKLILDDNNQSFSSSCEDGFSDLDLPEIEVRFSDED 257

Query: 208 VLVRIHSEKHEGLFAKLLGEMEKLNFTIVNGNVFPFGTSMQYMV 252
           VL++I  EK +G  AK++ E+EKL+  I N +V  FG ++   +
Sbjct: 258 VLIKILCEKQKGHLAKIMAEIEKLHILITNSSVLNFGPTLDITI 301

BLAST of Spg030032 vs. ExPASy Swiss-Prot
Match: Q1PF17 (Transcription factor bHLH18 OS=Arabidopsis thaliana OX=3702 GN=BHLH18 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.3e-34
Identity = 82/171 (47.95%), Postives = 115/171 (67.25%), Query Frame = 0

Query: 88  RKKRLMSKNPPRADDHVLAERKRREKLSQRFIALSAVLPGLKKMDKASVLGDAIEYIKQL 147
           ++ + ++++   A DH+LAERKRREKL+QRF+ALSA++PGLKKMDKASVLGDAI++IK L
Sbjct: 112 KRAQSLTRSQSNAQDHILAERKRREKLTQRFVALSALIPGLKKMDKASVLGDAIKHIKYL 171

Query: 148 KERVELLEEKAKNKSIESGILIKKS-------HSYEYDCGEDDDQKQVTRFSDQKLPEIE 207
           +E V+  EE+ K K++ES +L+KKS       H        D ++      S   LPEIE
Sbjct: 172 QESVKEYEEQKKEKTMESVVLVKKSSLVLDENHQPSSSSSSDGNRNS----SSSNLPEIE 231

Query: 208 VRISDKHVLVRIHSEKHEGLFAKLLGEMEKLNFTIVNGNVFPFGTSMQYMV 252
           VR+S K VL++I  EK +G   K++GE+EKL  +I N NV PFG +    +
Sbjct: 232 VRVSGKDVLIKILCEKQKGNVIKIMGEIEKLGLSITNSNVLPFGPTFDISI 278

BLAST of Spg030032 vs. ExPASy Swiss-Prot
Match: Q8S3F1 (Transcription factor NAI1 OS=Arabidopsis thaliana OX=3702 GN=NAI1 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.5e-27
Identity = 75/166 (45.18%), Postives = 104/166 (62.65%), Query Frame = 0

Query: 95  KNPPRADDHVLAERKRREKLSQRFIALSAVLPGLKKMDKASVLGDAIEYIKQLKERVELL 154
           + P    +HVLAERKRR+KL++R IALSA+LPGLKK DKA+VL DAI+++KQL+ERV+ L
Sbjct: 125 REPHLLKEHVLAERKRRQKLNERLIALSALLPGLKKTDKATVLEDAIKHLKQLQERVKKL 184

Query: 155 EEK--AKNKSIESGILIKKSH--------SYEYDCGE----DDDQKQVTRFSDQKLPEIE 214
           EE+     K  +S IL+K+S         SY   C           +V+ F  Q +P IE
Sbjct: 185 EEERVVTKKMDQSIILVKRSQVYLDDDSSSYSSTCSAASPLSSSSDEVSIFK-QTMPMIE 244

Query: 215 VRISDKHVLVRIHSEKHEGLFAKLLGEMEKLNFTIVNGNVFPFGTS 247
            R+SD+ +L+R+H EK++G   K+L  +EK    +VN    PFG S
Sbjct: 245 ARVSDRDLLIRVHCEKNKGCMIKILSSLEKFRLEVVNSFTLPFGNS 289

BLAST of Spg030032 vs. ExPASy Swiss-Prot
Match: Q1PF16 (Transcription factor bHLH19 OS=Arabidopsis thaliana OX=3702 GN=BHLH19 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 7.4e-27
Identity = 75/164 (45.73%), Postives = 102/164 (62.20%), Query Frame = 0

Query: 94  SKNPPRADDHVLAERKRREKLSQRFIALSAVLPGLKKMDKASVLGDAIEYIKQLKERVEL 153
           +++P  A +HVLAERKRREKLS++FIALSA+LPGLKK DK ++L DAI  +KQL+E++  
Sbjct: 111 TRSPVLAKEHVLAERKRREKLSEKFIALSALLPGLKKADKVTILDDAISRMKQLQEQLRT 170

Query: 154 L-EEKAKNKSIESGILIKKSHSYEYDCGEDDDQKQVTRFSDQKLPEIEVRISDKHVLVRI 213
           L EEK   + +ES IL+KKS  +  +         V    DQ LPEIE +IS   +L+RI
Sbjct: 171 LKEEKEATRQMESMILVKKSKVFFDEEPNLSCSPSVHIEFDQALPEIEAKISQNDILIRI 230

Query: 214 HSEKHEGLFAKLLGEMEKLNFTIVNGNVFPFGTSMQYMVFVAQV 257
             EK +G    +L  +E     I N  V PFG S   +  +AQ+
Sbjct: 231 LCEKSKGCMINILNTIENFQLRIENSIVLPFGDSTLDITVLAQM 274

BLAST of Spg030032 vs. ExPASy Swiss-Prot
Match: Q9ZPY8 (Transcription factor ABA-INDUCIBLE bHLH-TYPE OS=Arabidopsis thaliana OX=3702 GN=AIB PE=2 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 1.5e-14
Identity = 52/191 (27.23%), Postives = 96/191 (50.26%), Query Frame = 0

Query: 81  LMNVAEMRKKRLMSKNPPRADD----HVLAERKRREKLSQRFIALSAVLPGLKKMDKASV 140
           +++V + ++ R   + P    +    HV AER+RREKL+QRF AL +V+P + KMDKAS+
Sbjct: 370 IVSVVDEKRPRKRGRKPANGREEPLNHVEAERQRREKLNQRFYALRSVVPNISKMDKASL 429

Query: 141 LGDAIEYIKQLKERVELLEEKAKNKSIESGILIKKSHSYEYDCGEDDDQKQVTRFSDQKL 200
           LGDAI YIK+L+E+V+++E++                      G D    +    + ++ 
Sbjct: 430 LGDAISYIKELQEKVKIMEDER--------------------VGTDKSLSESNTITVEES 489

Query: 201 PEIEVRISDKHVLVRIHSEKHEGLFAKLLGEMEKLNFTIVNGNVFPFGTSMQYMVFVAQV 260
           PE++++  ++ V+VR+ S       ++++  M   N +++             +      
Sbjct: 490 PEVDIQAMNEEVVVRVISPLDSHPASRIIQAMRNSNVSLMEAK----------LSLAEDT 530

Query: 261 CFHLFISSCND 268
            FH F+   N+
Sbjct: 550 MFHTFVIKSNN 530

BLAST of Spg030032 vs. ExPASy TrEMBL
Match: A0A6J1JCQ1 (transcription factor bHLH25-like OS=Cucurbita maxima OX=3661 GN=LOC111483221 PE=4 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 3.5e-72
Identity = 169/269 (62.83%), Postives = 194/269 (72.12%), Query Frame = 0

Query: 2   EGQDSALLFH-QYQFNNSNTTCDS-HAFDEWRACGGRITSIPAAAAAASQIILFEEGKFC 61
           E QDS+LLFH QYQ  N+  T DS   F+ WR+ GG      A A+++SQ+ILF+EGKFC
Sbjct: 4   EEQDSSLLFHDQYQLFNATPTSDSPPLFNHWRSYGGS----AALASSSSQMILFQEGKFC 63

Query: 62  PAGLVEPKAEICGYGNGDFWPLMNVAEMRKKRLMSKNPPRADDHVLAERKRREKLSQRFI 121
           P   V   AEI        WPL+NV          +NPPRA DHVLAERKRREKLSQRFI
Sbjct: 64  P---VAAAAEI--------WPLVNV---------GRNPPRAKDHVLAERKRREKLSQRFI 123

Query: 122 ALSAVLPGLKKMDKASVLGDAIEYIKQLKERVELLEEKAKNKSIESGILIKKSH--SYEY 181
           ALSA+LPGLKKMDKAS+LGDAI+YIKQL+ERVE LEE  KN +IES ILIKKSH  +   
Sbjct: 124 ALSALLPGLKKMDKASILGDAIKYIKQLQERVESLEEMGKNSNIESAILIKKSHLCNNMN 183

Query: 182 DCGEDD--DQKQVTRFSDQKLPEIEVRISDKHVLVRIHSEKHEGLFAKLLGEMEKLNFTI 241
           DC + D  +Q   TRFSDQKL EIE +ISDKHVLVRIH EKHEGL A+LL EMEKLNF I
Sbjct: 184 DCDDIDGEEQNHKTRFSDQKLVEIEAKISDKHVLVRIHCEKHEGLLARLLEEMEKLNFRI 243

Query: 242 VNGNVFPFGTSMQYMVFVAQVCFHLFISS 265
           +N NVFPFG+S QYMVF+ Q   + FI S
Sbjct: 244 INANVFPFGSSTQYMVFLLQRNSNSFIDS 248

BLAST of Spg030032 vs. ExPASy TrEMBL
Match: A0A6J1ETR4 (transcription factor bHLH25-like OS=Cucurbita moschata OX=3662 GN=LOC111435876 PE=4 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 2.3e-71
Identity = 161/261 (61.69%), Postives = 187/261 (71.65%), Query Frame = 0

Query: 2   EGQDSALLFH-QYQFNNSNTTCDS-HAFDEWRACGGRITSIPAAAAAASQIILFEEGKFC 61
           E QDS+LLFH QYQ  N+  T DS   F+ WR+CGG      A A+++SQ+ILF+EGKFC
Sbjct: 4   EEQDSSLLFHDQYQLFNATPTSDSPPLFNHWRSCGGS----AAVASSSSQMILFQEGKFC 63

Query: 62  PAGLVEPKAEICGYGNGDFWPLMNVAEMRKKRLMSKNPPRADDHVLAERKRREKLSQRFI 121
           PA               + WPL+NV          +NPPRA DHVLAERKRREKLSQRFI
Sbjct: 64  PAA-------------AEIWPLVNV---------GRNPPRAKDHVLAERKRREKLSQRFI 123

Query: 122 ALSAVLPGLKKMDKASVLGDAIEYIKQLKERVELLEEKAKNKSIESGILIKKSH--SYEY 181
           ALSA+LPGLKKMDKAS+LGDAI+YIKQL+ERVE LEE  KN +IES I IKKSH  +   
Sbjct: 124 ALSALLPGLKKMDKASILGDAIKYIKQLQERVESLEEMGKNSNIESAIFIKKSHLCNNMN 183

Query: 182 DCGED---DDQKQVTRFSDQKLPEIEVRISDKHVLVRIHSEKHEGLFAKLLGEMEKLNFT 241
            C +D   ++    T FSDQKL E+E +ISDKHVLVRIH EKHEGL AKLL EMEKLNF 
Sbjct: 184 GCCDDIDGEEHNHKTGFSDQKLVEMEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFK 238

Query: 242 IVNGNVFPFGTSMQYMVFVAQ 256
           I+NGNVFPFG+S QYMVF+ Q
Sbjct: 244 IINGNVFPFGSSTQYMVFLLQ 238

BLAST of Spg030032 vs. ExPASy TrEMBL
Match: A0A6J1C4D8 (transcription factor bHLH18-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008381 PE=4 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 2.3e-55
Identity = 147/254 (57.87%), Postives = 166/254 (65.35%), Query Frame = 0

Query: 17  NSNTTCDSHAFDEWRACGGRITSIPAAAAAASQIILFEEGKFCPAGLVEPKAEICGYGNG 76
           N+N TCDS AF                  AAS     EE  F       P AEI    +G
Sbjct: 2   NNNNTCDSRAF-----------------VAAS-----EEPTFS-----RPTAEIICSSHG 61

Query: 77  ---DFWPLMNVAEMRKKRLMSKNPPRADDHVLAERKRREKLSQRFIALSAVLPGLKKMDK 136
               F P      + +KR  +     A DHVLAERKRREKLSQRFIALSA+LPGLKKMDK
Sbjct: 62  MHTSFSPPSINNNIPEKRKPTPTTAHAQDHVLAERKRREKLSQRFIALSALLPGLKKMDK 121

Query: 137 ASVLGDAIEYIKQLKERVELLEEKAKNKSIESGILIKKSHSYEYDCGEDDDQKQVTRFSD 196
           ASVLGDAI+YIKQ++ERV +LEEKAK +SIES IL+ KSH Y  DC E     +++RFSD
Sbjct: 122 ASVLGDAIKYIKQVEERVRVLEEKAKKRSIESAILVNKSHLYN-DCDE-----EISRFSD 181

Query: 197 QK-LPEIEVRISD--KHVLVRIHSEKHEGLFAKLLGEMEKLNFTIVNGNVFPFGTSMQYM 256
           QK LPEIE RISD   +VLVRIH EK+EGLFAKLLGEMEK+N TIVN N FPFGTS QYM
Sbjct: 182 QKQLPEIEARISDDKNNVLVRIHCEKNEGLFAKLLGEMEKMNLTIVNANFFPFGTSTQYM 222

Query: 257 VFVAQVCFHLFISS 265
           V VAQ+   L I S
Sbjct: 242 VLVAQIDSELKIKS 222

BLAST of Spg030032 vs. ExPASy TrEMBL
Match: A0A6J1C590 (transcription factor bHLH18-like isoform X4 OS=Momordica charantia OX=3673 GN=LOC111008381 PE=4 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 2.3e-55
Identity = 147/254 (57.87%), Postives = 166/254 (65.35%), Query Frame = 0

Query: 17  NSNTTCDSHAFDEWRACGGRITSIPAAAAAASQIILFEEGKFCPAGLVEPKAEICGYGNG 76
           N+N TCDS AF                  AAS     EE  F       P AEI    +G
Sbjct: 2   NNNNTCDSRAF-----------------VAAS-----EEPTFS-----RPTAEIICSSHG 61

Query: 77  ---DFWPLMNVAEMRKKRLMSKNPPRADDHVLAERKRREKLSQRFIALSAVLPGLKKMDK 136
               F P      + +KR  +     A DHVLAERKRREKLSQRFIALSA+LPGLKKMDK
Sbjct: 62  MHTSFSPPSINNNIPEKRKPTPTTAHAQDHVLAERKRREKLSQRFIALSALLPGLKKMDK 121

Query: 137 ASVLGDAIEYIKQLKERVELLEEKAKNKSIESGILIKKSHSYEYDCGEDDDQKQVTRFSD 196
           ASVLGDAI+YIKQ++ERV +LEEKAK +SIES IL+ KSH Y  DC E     +++RFSD
Sbjct: 122 ASVLGDAIKYIKQVEERVRVLEEKAKKRSIESAILVNKSHLYN-DCDE-----EISRFSD 181

Query: 197 QK-LPEIEVRISD--KHVLVRIHSEKHEGLFAKLLGEMEKLNFTIVNGNVFPFGTSMQYM 256
           QK LPEIE RISD   +VLVRIH EK+EGLFAKLLGEMEK+N TIVN N FPFGTS QYM
Sbjct: 182 QKQLPEIEARISDDKNNVLVRIHCEKNEGLFAKLLGEMEKMNLTIVNANFFPFGTSTQYM 222

Query: 257 VFVAQVCFHLFISS 265
           V VAQ+   L I S
Sbjct: 242 VLVAQIDSELKIKS 222

BLAST of Spg030032 vs. ExPASy TrEMBL
Match: A0A6J1C8F2 (transcription factor bHLH18-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111008381 PE=4 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 2.3e-55
Identity = 145/246 (58.94%), Postives = 163/246 (66.26%), Query Frame = 0

Query: 17  NSNTTCDSHAFDEWRACGGRITSIPAAAAAASQIILFEEGKFCPAGLVEPKAEICGYGNG 76
           N+N TCDS AF                  AAS     EE  F       P AEI    +G
Sbjct: 2   NNNNTCDSRAF-----------------VAAS-----EEPTFS-----RPTAEIICSSHG 61

Query: 77  ---DFWPLMNVAEMRKKRLMSKNPPRADDHVLAERKRREKLSQRFIALSAVLPGLKKMDK 136
               F P      + +KR  +     A DHVLAERKRREKLSQRFIALSA+LPGLKKMDK
Sbjct: 62  MHTSFSPPSINNNIPEKRKPTPTTAHAQDHVLAERKRREKLSQRFIALSALLPGLKKMDK 121

Query: 137 ASVLGDAIEYIKQLKERVELLEEKAKNKSIESGILIKKSHSYEYDCGEDDDQKQVTRFSD 196
           ASVLGDAI+YIKQ++ERV +LEEKAK +SIES IL+ KSH Y  DC E     +++RFSD
Sbjct: 122 ASVLGDAIKYIKQVEERVRVLEEKAKKRSIESAILVNKSHLYN-DCDE-----EISRFSD 181

Query: 197 QK-LPEIEVRISD--KHVLVRIHSEKHEGLFAKLLGEMEKLNFTIVNGNVFPFGTSMQYM 256
           QK LPEIE RISD   +VLVRIH EK+EGLFAKLLGEMEK+N TIVN N FPFGTS QYM
Sbjct: 182 QKQLPEIEARISDDKNNVLVRIHCEKNEGLFAKLLGEMEKMNLTIVNANFFPFGTSTQYM 214

BLAST of Spg030032 vs. TAIR 10
Match: AT4G37850.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 151.8 bits (382), Expect = 8.1e-37
Identity = 82/164 (50.00%), Postives = 114/164 (69.51%), Query Frame = 0

Query: 88  RKKRLMSKNPPRADDHVLAERKRREKLSQRFIALSAVLPGLKKMDKASVLGDAIEYIKQL 147
           ++ +  S+N   A DH++AERKRREKL+QRF+ALSA++PGLKKMDKASVLGDA+++IK L
Sbjct: 138 KRAQPFSRNQSNAQDHIIAERKRREKLTQRFVALSALVPGLKKMDKASVLGDALKHIKYL 197

Query: 148 KERVELLEEKAKNKSIESGILIKKSHSYEYDCGEDDDQKQVTRFSDQKLPEIEVRISDKH 207
           +ERV  LEE+ K + +ES +L+KKS     D  +         FSD  LPEIEVR SD+ 
Sbjct: 198 QERVGELEEQKKERRLESMVLVKKSKLILDDNNQSFSSSCEDGFSDLDLPEIEVRFSDED 257

Query: 208 VLVRIHSEKHEGLFAKLLGEMEKLNFTIVNGNVFPFGTSMQYMV 252
           VL++I  EK +G  AK++ E+EKL+  I N +V  FG ++   +
Sbjct: 258 VLIKILCEKQKGHLAKIMAEIEKLHILITNSSVLNFGPTLDITI 301

BLAST of Spg030032 vs. TAIR 10
Match: AT2G22750.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 148.3 bits (373), Expect = 9.0e-36
Identity = 82/171 (47.95%), Postives = 115/171 (67.25%), Query Frame = 0

Query: 88  RKKRLMSKNPPRADDHVLAERKRREKLSQRFIALSAVLPGLKKMDKASVLGDAIEYIKQL 147
           ++ + ++++   A DH+LAERKRREKL+QRF+ALSA++PGLKKMDKASVLGDAI++IK L
Sbjct: 112 KRAQSLTRSQSNAQDHILAERKRREKLTQRFVALSALIPGLKKMDKASVLGDAIKHIKYL 171

Query: 148 KERVELLEEKAKNKSIESGILIKKS-------HSYEYDCGEDDDQKQVTRFSDQKLPEIE 207
           +E V+  EE+ K K++ES +L+KKS       H        D ++      S   LPEIE
Sbjct: 172 QESVKEYEEQKKEKTMESVVLVKKSSLVLDENHQPSSSSSSDGNRNS----SSSNLPEIE 231

Query: 208 VRISDKHVLVRIHSEKHEGLFAKLLGEMEKLNFTIVNGNVFPFGTSMQYMV 252
           VR+S K VL++I  EK +G   K++GE+EKL  +I N NV PFG +    +
Sbjct: 232 VRVSGKDVLIKILCEKQKGNVIKIMGEIEKLGLSITNSNVLPFGPTFDISI 278

BLAST of Spg030032 vs. TAIR 10
Match: AT2G22750.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 147.1 bits (370), Expect = 2.0e-35
Identity = 84/182 (46.15%), Postives = 118/182 (64.84%), Query Frame = 0

Query: 88  RKKRLMSKNPPRADDHVLAERKRREKLSQRFIALSAVLPGLKKMDKASVLGDAIEYIKQL 147
           ++ + ++++   A DH+LAERKRREKL+QRF+ALSA++PGLKKMDKASVLGDAI++IK L
Sbjct: 112 KRAQSLTRSQSNAQDHILAERKRREKLTQRFVALSALIPGLKKMDKASVLGDAIKHIKYL 171

Query: 148 KERVELLEEKAKNKSIESGILIKKS-------HSYEYDCGEDDDQKQVTRFSDQKLPEIE 207
           +E V+  EE+ K K++ES +L+KKS       H        D ++      S   LPEIE
Sbjct: 172 QESVKEYEEQKKEKTMESVVLVKKSSLVLDENHQPSSSSSSDGNRNS----SSSNLPEIE 231

Query: 208 VRISDKHVLVRIHSEKHEGLFAKLLGEMEKLNFTIVNGNVFPFGTSMQYMVFVAQVCFHL 263
           VR+S K VL++I  EK +G   K++GE+EKL  +I N NV PFG +    +      F +
Sbjct: 232 VRVSGKDVLIKILCEKQKGNVIKIMGEIEKLGLSITNSNVLPFGPTFDISIIAQNNNFDM 289

BLAST of Spg030032 vs. TAIR 10
Match: AT2G22770.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 124.8 bits (312), Expect = 1.1e-28
Identity = 75/166 (45.18%), Postives = 104/166 (62.65%), Query Frame = 0

Query: 95  KNPPRADDHVLAERKRREKLSQRFIALSAVLPGLKKMDKASVLGDAIEYIKQLKERVELL 154
           + P    +HVLAERKRR+KL++R IALSA+LPGLKK DKA+VL DAI+++KQL+ERV+ L
Sbjct: 125 REPHLLKEHVLAERKRRQKLNERLIALSALLPGLKKTDKATVLEDAIKHLKQLQERVKKL 184

Query: 155 EEK--AKNKSIESGILIKKSH--------SYEYDCGE----DDDQKQVTRFSDQKLPEIE 214
           EE+     K  +S IL+K+S         SY   C           +V+ F  Q +P IE
Sbjct: 185 EEERVVTKKMDQSIILVKRSQVYLDDDSSSYSSTCSAASPLSSSSDEVSIFK-QTMPMIE 244

Query: 215 VRISDKHVLVRIHSEKHEGLFAKLLGEMEKLNFTIVNGNVFPFGTS 247
            R+SD+ +L+R+H EK++G   K+L  +EK    +VN    PFG S
Sbjct: 245 ARVSDRDLLIRVHCEKNKGCMIKILSSLEKFRLEVVNSFTLPFGNS 289

BLAST of Spg030032 vs. TAIR 10
Match: AT2G22760.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 5.3e-28
Identity = 75/164 (45.73%), Postives = 102/164 (62.20%), Query Frame = 0

Query: 94  SKNPPRADDHVLAERKRREKLSQRFIALSAVLPGLKKMDKASVLGDAIEYIKQLKERVEL 153
           +++P  A +HVLAERKRREKLS++FIALSA+LPGLKK DK ++L DAI  +KQL+E++  
Sbjct: 111 TRSPVLAKEHVLAERKRREKLSEKFIALSALLPGLKKADKVTILDDAISRMKQLQEQLRT 170

Query: 154 L-EEKAKNKSIESGILIKKSHSYEYDCGEDDDQKQVTRFSDQKLPEIEVRISDKHVLVRI 213
           L EEK   + +ES IL+KKS  +  +         V    DQ LPEIE +IS   +L+RI
Sbjct: 171 LKEEKEATRQMESMILVKKSKVFFDEEPNLSCSPSVHIEFDQALPEIEAKISQNDILIRI 230

Query: 214 HSEKHEGLFAKLLGEMEKLNFTIVNGNVFPFGTSMQYMVFVAQV 257
             EK +G    +L  +E     I N  V PFG S   +  +AQ+
Sbjct: 231 LCEKSKGCMINILNTIENFQLRIENSIVLPFGDSTLDITVLAQM 274

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022985138.17.3e-7262.83transcription factor bHLH25-like [Cucurbita maxima][more]
XP_023552894.11.2e-7162.36transcription factor bHLH25-like [Cucurbita pepo subsp. pepo][more]
XP_022929215.14.7e-7161.69transcription factor bHLH25-like [Cucurbita moschata][more]
KAG7015418.11.1e-5668.28Transcription factor bHLH25, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_022136753.14.7e-5558.94transcription factor bHLH18-like isoform X3 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9T0721.1e-3550.00Transcription factor bHLH25 OS=Arabidopsis thaliana OX=3702 GN=BHLH25 PE=2 SV=2[more]
Q1PF171.3e-3447.95Transcription factor bHLH18 OS=Arabidopsis thaliana OX=3702 GN=BHLH18 PE=1 SV=1[more]
Q8S3F11.5e-2745.18Transcription factor NAI1 OS=Arabidopsis thaliana OX=3702 GN=NAI1 PE=2 SV=1[more]
Q1PF167.4e-2745.73Transcription factor bHLH19 OS=Arabidopsis thaliana OX=3702 GN=BHLH19 PE=1 SV=1[more]
Q9ZPY81.5e-1427.23Transcription factor ABA-INDUCIBLE bHLH-TYPE OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1JCQ13.5e-7262.83transcription factor bHLH25-like OS=Cucurbita maxima OX=3661 GN=LOC111483221 PE=... [more]
A0A6J1ETR42.3e-7161.69transcription factor bHLH25-like OS=Cucurbita moschata OX=3662 GN=LOC111435876 P... [more]
A0A6J1C4D82.3e-5557.87transcription factor bHLH18-like isoform X1 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1C5902.3e-5557.87transcription factor bHLH18-like isoform X4 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1C8F22.3e-5558.94transcription factor bHLH18-like isoform X3 OS=Momordica charantia OX=3673 GN=LO... [more]
Match NameE-valueIdentityDescription
AT4G37850.18.1e-3750.00basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT2G22750.29.0e-3647.95basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT2G22750.12.0e-3546.15basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT2G22770.11.1e-2845.18basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT2G22760.15.3e-2845.73basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 137..164
NoneNo IPR availablePANTHERPTHR45959BHLH TRANSCRIPTION FACTORcoord: 86..256
NoneNo IPR availablePANTHERPTHR45959:SF36TRANSCRIPTION FACTOR NAI1coord: 86..256
NoneNo IPR availableCDDcd11452bHLH_AtNAI1_likecoord: 100..172
e-value: 7.30233E-35
score: 118.335
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 104..153
e-value: 1.1E-14
score: 64.8
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 103..148
e-value: 4.6E-10
score: 39.3
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 98..147
score: 16.291254
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 89..162
e-value: 2.5E-16
score: 61.3
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 102..167

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg030032.1Spg030032.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0110165 cellular anatomical entity
molecular_function GO:0046983 protein dimerization activity