Spg030021 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg030021
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	subtilisin-like protease SBT3.7
Location	scaffold6: 8786657 .. 8795553 (+)
RNA-Seq Expression	Spg030021
Synteny	Spg030021

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAACAGCGAAGCTCCTTTGATCGTTTCTGTTATGAGATTTTATGTTTTGTTTTCAATGTTTGCTTACAAATCCATGGCAGAAGAAGCAGACCAAAACCGCAAGGTACGTACGCCCACCACCCACTGTAATTCAACTCTTTTATTATTATTTCTTTGTTTTTTGCTTTTGGTGGATGCCCATATTGTAGATTAAATTTGATCAATTGGATTCCTGGATGTGTTCAGGTTCACATCGTTTACCTTGGAGAAAGGCAATACGATGATATTAATTTAACAACTGAGTCTCACCATAATTTACTGGCCAAAGTATTGGGAAGGTAACCTCTTTGGTTCATTTTTTTAAAATTTTTTTTGTTGATTTAATTTGCTGACAAACTTTTTGTACAAAAAAAAATGTGTTCGTTTTGATTTGGATAATGAAGCAAGGAGAAGTCATTGGAATCAATGGTGTACAGTTATAGACATGGCTTTTCTGGGTTTGCAGCCAAGCTCACCAAGTCTCAGGCTCAAAAGCTAGTCGGTAAACAACAATACTTTGATTGCATACCATACCAGTGTTGAGGTCCACCTACAACACCACAATTAAAATGTTGATAAGTTGGAGTGATAATTTTTTTTTTTTTTTTTGATCATTTTCTACTTTTCTCTTTCTTGGTTGTCAAGATTTTAATGGAGTGTGTTGTATATGCAGTGAGTATTATTCATATACATATATATGTATAACATAAGGAAGTTGACCAGATTGATTTTTTTTTTTTTTTAGTGACCAAATCGAGAGTTAGTCAATGCATTAACCATCACTAAATTTTCCAACTTTTCTATTTGGTTCCATAGTTAAATATTTCTTGTAAGTCCTAAAGTTTTTAAGTTGATGTCTATGTGGTTGTTATACTTTAAAAATAGTTTTTTAAAAGGTTTTTTGAAGTTCCAAGGATTGTATCTAAATGATATTTTAGTGCACTTCAAAGCCACATCCATCTAGATTCTTATTTTTTTTTCTTTTAAATTTTACGATTAATCCGTTTTTAAACTTTCAATTCTACTATTATGAACGACGAACGAAACTACAGAAATACCAGGGGTGATTCGAGTTTTTCCAAGTTCACTTTACAAATTGCATACTACAAGAAGTTGGGATTTTCTCGGCCTCTCCTCTTCTCCCTTTGAGTCATCCAACCTTCTTCATCGTGCTAAAATGGGCGACAATGTCATTATAGGTGTCATTGATACAGGTACATCTTTGCCTCTAGTCTCTCAAAATAATTCACCACCTCATATAATATGGTGAAACTTCTTTGATATTTATCAAAATTGAAAAAGACGAGTTGGAGAGAAAAATTTTAAGGTTAAATTACAAGTTCGGTCCCTGAACTTTCAAGATTGTATCAATTTAGTCCCTAAACTTTAAAAAATGTCTAATAGGTCCTTAAACTTTCAATTTTGTGCTTAATAGGTTTCTAAACTTTAAAAATGTCTAATAGGTTCCTGACATATTTAAGATAGAACAATACTTGGTAGGATCTCCCTCCACATACCTCATTAAAATCTTGGCAACTAAGAAAGAGAAGGATTTCTCTCCTAGTTATGTCAACATTTTAAGGGTGGTATGGAGGGAGATCACTGTTATGCAACCAAGTATTTTCCTTTTAAAAATTAACATAAAAAATTGAATTTTATGTCTATTGGGATCCTAAACTTTCAATTTTGTATCTAGTAGGCTTGTAAATTAAAAAAAAAATTGAACAGGTCAGGAATTTATTAGACACAAAATTGAAAGCTGAGAGACCTATTGAACACAACAAAAGTGAAATTATAAGAACCTACAAAATACTTTTTAAAGTTCAGGGACTTATGGGACACAAAATTGAAAGTTTAAGGAACTATTTGACACTTTTTAAAGTTTAGAGATCAAATTGACAAAACCAAGTTTAGGGACTAAACTTGTAACTGAACTAAATTTTAAAGTCGACTAACCTTGTTAAAGTATTCAAATAAAATTAAGTTTACCGTAACCGATTAGCTTAAGCTTTTGGGTTTAGTGGTGATTTAAGATGGTATTCTAGTAGGAGGTTCTGTGTTCGGACTCCTTGTAATGTCATTTCTTGTCCAATTAATATGAATTTTTGGAAAGTCTTAAAATATTAATATAAATTCAAGTTTACCGTTTAAGTTTTGAGTTCAGTGGTGATTTCACACAGTCGACTTGATTAGGATTTTGGCCGGAGTCCGCGTCTTTTGCAGACAAAGGAATGGGACCAGTACCATCACGATGGAAAGGCAAATGCAAATCAGGAGAATTGTTCAATTCCACAAACTGCAACAAGAAAGTCATAGGAGCACGCTGGTTCGTGAAGGGCTTCATCGCCGATTATGGGCGGGAGGCACTAGCCAACGAATGCTTATCCCCACGAGACACCCATGGACACGGAACCCACACAGCCAGCACAGCTGCAGGTTCATTTGTAACAAATGTTAGCTACCACGGCCTTGGTGCCGGTACGTTGAGGGGCGGTGCGCCACTCGCACGGTTGGCCATATACAAGGTAGGGTGGACCGGTGGTGGCGGGTCGATGGCGGACATATTGAAGGGCATAGATGTGGCCATTCACGACGGTGTGGATGTGCTGTCTATATCGATGGGGCTGCCACTTCCTTTGGTTCCAGAGTTGGACCAAGCAAATGGAATTGGCATTGGGTCATTTCATGCTATTGCGAGGGGGATTCCTGTTGTGTGTTCGGGTGGCAATGAAGGCCCTTATGAGCAGACGGTGGGAAACACTGCGCCTTGGATTTTGACTGTGGCTGCCTCTACAATGGATAGAGCCTTTCTTTCATCAATTACTCTTCTTGAAGATAATACCACTTATTTGGTATATACAAATATATAGTTATATTGCCCCATTTATGTGTCACAGCCTTTTAAAGTTGTTGCATTGAAAGTGGAAGGTTTTTACTTGACACTTGACAGGGCCAAAGCTTCTTCTATTCCAAAAAGGATCTTGTTGCTGAGTTGACCGTTCCGCCAGATAGAAGGTTGGTTTTGGTTAACCTTTTTTTTTCTTTTTTTGGTTTAATTTTGCATTTTGATAGTTCAAACTTTTCAGTTTATTTCTTCATTTTTGTTTTTTTTTTTCCAAATAGATAATTTTATTATTATTTTTTTTTTTGACAACCACGAATAGATAAAATAGTTTGTGTTGTATATTAGAGTGAAAAAAACAACAGGCAACTATTGCATATCTTTTAAAAGTATAATTAAATGTTTTCTTTTTCTTTTGATGTAATAGATACTTCAAAAGTTTGTGAATAAAAATAGTTATTTAAAGAAAATAGAGATTGAAATAGATGATTTGAAAGTTAACCACCACATCACAACTTAAAGATAAAATTTAAGCAATTGTTGTATTATTGGATTAGTTACATCTTTACATATATATTGTATGTGTTTCATTGGATTTAATTTGGAGAGCAGATGTGATCGCATTTCGGGAAATGAGAATTTTAGTGGGAAAGTTGTTCTCTGCTTCTCTGATAAAGTTAATCTGTCGGCGGCAACGGCGGTGAAAAAAGCAAATGGGAGTGGGATAATTGTCGCCGGCGAAAACATCGATATATTGTATGAGTTTGGCGAATCCTTTCCATGCGTCGTAGTGGATACCGATGTTGGCACAAAATTGTTCTTTCAAGCTTTAGACGACAGGTAATTCAATCATTCATTCAAGAGGAAACAAAATGAATGACATAGATTAAAAATATACAAAAATGTCTATGAATTTTAGGGTAGATTTCAATAATGTTCTTCAACTTTCAAAAGTTTCACATTAAATTCAACTTTTTCATTAAAATGGAGAAAAAATTTACGTGATAGTAAGTTGTAAAAATAGACGAAGAATTACACACAAGCACAAATTAGCCAAGTTTCAATCATATATTCCTAATCTTTGTAGTGAGTTTCATACTTGTGATTTCAAAAGTTTTATTTTGATTTTAACCATTGAAATTGTTTCAAAACAACCCTTATGTATTGGTTCTGTTGTTAAAATAAAGATGGATATATGATATGTCGTAGTGATCTATAAAAACTCAAGGAAGGAATAAGCAAAGAAACTCAAGACAATGAAACATAGGGACAACTAATTACCACTACGGATTCACGCTTTTCATCGACTCTTCATCAATCAATAGTTTTCTATATTCTTCTTCTTACTTCTATGGATTCACGCTTTCCACCGACTCTTCAACAAAATTTCTCACATAAACATATTTTTCTCCTCTTTTTTTGGAAAACTTATTAATTGTTCTTGTATAATGAGACTCCATTTTTTAAATCGTCCGATGTAAATCTTCAAATTTGTTTTTCCCTAATAACCATTTGATAGAAAATCAAGATACTTTTGTTATTTCTCTTTCTTACATCTAACGGTACAAAACTATCGGTTTCATTGCACTGAGAGCACCTAAAAGTTTCTCCCTTTTTTTGACCTATCTTTGACCGTAGCTTTCCATGATATTCTTGGCTTTCATAGTCGATGAGGTCATTGATTTTTTTTTTCGACCCTTCTTTGACCGTAGCCCTTCCAATATTTTTTACCCTTCTTTGACTGTAGTTGTGTTGTTAAACTAAAGATGGAAATTGATTTTCTGTTTTTTAAAATTGTTTTTGAAATTATAAACAAAATTTAAAAAAAAAAAAAATGTAGTTTTTAAAAACAGATTTCTGTTTTACAAAAACTCATTTTTTCTTCTATAGCTCATTTTCTCTCTTTACATATCTTTTGCCAACAAATTCTTTTTCTATCTTTCTCTCTCTAAAACGGTTTTTTCTCTACAAATTTTCTCTCTCTATAAAATTTTATCTCAAAATAAATTTTTATGTCACTCTCTTTCTAAAAAATTCATCTTTACTTACTTTTTTTTCTATAAAATTTTTAACTTTATTTAACTTTCATTTCTATTTCTTTTCTAATAACATATCTCTCTAATTTATCTCCTTAAAAATCAAATATTTTTCTTTTCCGTAAATTTTTCTCTTTCAAAACTTTTTTTTTCTCTACAACTTATCTATTTTTTTAACATTTCTCGATTACTTTTCTCTCCTTGATAAACATATCTCTAACTTTTCTGTTGAAAAAACAAATTCCCTAAATTTTGTCTTGCTACAATTTTTATTTAATTTTCTAATTTATTGTTATTTCTTTAATTAATATATTTTTTACTCTTAACCTTACTATCTCTCTAAAAGTTCTCTTTTTATTTAATTTTTCTCTCTAAAATTTATCTTTTCTACTCTAACTTTTTGTTTTTTTTTCTCAAACTAATATTTTTTTATGGAGTCTAGCTAATAACTCTCCCTCCAAATATTTAATTCCATGTCTCTAAATTACTATGTTTAGCTTTTCTCTTGAAAACATTCATATTCATGTCTCTAAATTTTTATTTAAATATCTCCACACATTTTTCTATCTCAAAAACATCTTTTCATCTCTTTAATTTTTTAAAAACTAAAAATTAAAAACAGTTATCAAATAAGTTTGGTTTTCTGTTTTTTAGAAACAGAAAAAAAAAAAACAGTTATCAAACATATATAGTTTTTGTGTTTTAAAAACAGAAAAAAGAAAACAAAAAACCAAAAATAAAAAATTGAAATGGTTATTATATGGAACCAGAAACTTGTTAACACTTAAAGAATTATTTGACCTTCCAAAAAAAAAAAAAAGAATTATTTGACAAAAGATTAAAAATTTAGGAACTATTATTAGATACTTATTAAAATTCGTAGGGTAAGCACAAACTTGACCATTTAGTTTACACATCTTATTAAAAATCTCCCAAAATTTTCTTTCGCTACAACTTTTCTTTATCAAATTTTCTTTTTCTTTAGCATATCTTTTACTCTTAACCTTACTATCACTCTAAAATTTATCTTTTCTACTCTAAATTTTTTCTTTCTTTCTCTAACTAACATCTTTTATGGAGTCTAACTAATATTCTCTCTCTAAACATTATCTTTCATGTCTCTAAATTTTCTATGTTTAACTTTTCTCTCAAAAACATTTATATTCGAGTCTCTAAATTTTTGTTTCAATGTCTCTACATATTGTTCTCTCTCCAAAATATCTTTTGATCTCTCTAATTTTTTAAAAACTGAAAACCAAAAACAGTTATCAAACAAGTTTGGTTTTCTGTTTTTAAAAACAAAAAACAAAAAACAGTTATAAAACATATATGGTTTTTGTTTTTAAAAAACAAAGAATCAACCAAAAACAGAAAAACCAAAAACCCAAAACACAGAAATGGTTATCAAAGGGGCCTTAGTGTTTGGTTTTTAGTTTTTGAAAATTAAAACTTACAAAAACACTACTTTTTTATGTCAGTTGAGCTATGTTCATGTTGGCTATAAGTTGTTTGTTTTGTCATCTACCTTATTTTCGTGTTTTCAAAAACCAAGTTAAAATTTGAAACAAAAAAAAAGTATTAAAGTAATTTTCAAAATCTTGTTTTTGTTTTTGGAATTTGGCTAAGAATTCAAGTGTGACCTTATGTTAAGATGAAAGTTAGTGTAAGAAAATTTGGAAAAAAAAACAAGAATAATTTCCAAAAACCGAAAACTAGATGGTTATTAAACGGACCTATTAGAGACGACTAAACTTGTAATTTAAGTATATGAATCTAATTTTATGAATGTTGTATGTGGTATCGTTTGCCTCTAATTTAATTTTTGCAAAATTGTAAAGTAATCCAATGGTAAGGTTGAGGCATGCAAAAACCATCATCGGCAAGCCCATATCAACCAGGATTGCCCATTTCTCATCTAGAGGTCCAAATTCTGCTTCACCTGCAATTCTCAAGGTGCATTATATTTTTCTCTCATCCTTTTCATTTGTGCTCCTTAAGTTTAAATTTTTAAATTTTATCTTTAAACTTTGATATTTCTCCATGTACTTCTTAAACTTTAAAAATGATCCATTATATACTTTTCAAAAAAAAAAAAAAACCATTATATATCTCTCTCTATGTATATATTATTTTACTGTTATTCTAATAACGTTGACAGACATATTTACTAATCTAGAGGATGGGGGATTGAATCTCTGATCTCTTGGTCATGATACAAACTTTATGTCAGTTAAGTCATGCTTTGGCAGACAATAAAAAAGAATTTTATGATAAGACGGGACCAAATACACAATCTAATTAAATTGATATTTTTACCCACCCACCCTTATGTTATCTATTTTTTTATGGTGTATTTTATATAGTTGATATACTCTCTAGTATCAAATCCCTATAATATCATCAATATATTGTCTAATAACATATCACAAAGCTAATGAATGATACTAGAAATATCAACTTATTAGAATTTAAATTAAAAGCTAAGGTCTCGTTTGATAATCATTTGGTTTGAAAATTATGCTTGTTTTTTTTTTTTTCCAATTTTCCTTCAATGATTTTCATCTTTTGTTGAGGAGACACATTTGAAGTCTTAGCCAAATTCCAAAAAAAAAAAAAAAAATGGAAACTACTTTTTTTTAGTTTTCAAAACTTGAATTGGTTTTTGAAATCTTTGAAAGAAGATAGATAACAAAACAAAGAAACTTATAGGTTGGAGAAATGTTATAAGCTTAAATTTTCAAAAACGAAAATCTAAAAAGTAAATGGTTATCAAACGGTACCTAATTGTCTTGTACATGAGCAGCCAGACATAGCCTCTCCTGGATCCAACATTCTAGCTGCCGTTCCTCCTGACTTTAGTTCAAACGACGAAGGGTTTGGCATGATGTCAGGAACTTCCATGGCTGCACCTCATGTCTCTGGCATCGTAGCTCTTCTTAAATCCCTACATCCTACTTGGTCACCTGCCGCCATTAAATCAGCCATCATCACCACGGGTATGTACATGTTATCAATAATTTAATTTATTAAGAAAACTATACAACTATTTTTTTTAAAACAAACTAATGCGTCCTTTGAAGAGACGCAATTTTGGGGATGAATGTGGTTGCATCCTTCTAAGATGGAAAATTACTATGTATGTCTGTGCAATGAGACTCTCTCTTTAAAACTGTTGGATGTAAATCTTTAAATTTGTTGGCCCAGTAACAGTTGGATACAAAATTGCAACTGGAGCACCTAAAAATTTCTCTTCTAAGAGACACAACACACGATTTTGTAAAATAGTTGTAAGACTATTTTTTTAATATAATTTTTGAAAAAATAAATTTGTGTAATCTTAATAACCCTGTAAATAACAAATTGTAGTAATATACACCTTTAAAAAAAATCGTAATTTAATTTATACACTTTGCATTTAATTTCCCACTTTTCACAAATGTAGCACGTGTAAAGAACCCCTCAGGATTGCCCATTTTTGCCAGCGGCAGTCCTCCCAAAGTCGCCGACCCATTTGACTACGGTGGTGGAGTGGTGGATCCCAACGCCGCCGCCGACCCAGGTCTGATCTACGATCTGGGCACCAGTGATTACATATATTATTACCTCTGCGCCATGGGTTATAAGGTTTCAGACATCTCTCACCTAACCAAGAAAAAAACAGAGTGTTCCTTGCAGAGACCTTCGATGTTGGACTTGAACCTGCCGACCATTACAGTCCCTGAACTCACAAACTCCACCACCGTGACTCGAACTGTGACCAATGTAGGGAACTTGACCTCCATTTATAGGGCAGTGGTCGAGCCTCCCCCTAGCACCAAGGTCAGTGTGGAGCCCCAGGTGTTGGCCTTCAACTCCAAAGTGAAAAAGATTTCCTTTAAGGTTACGATCTCCACCACCCTGTCTACGAACTACGGGTACTCTTTTGGAACCTTGACATGGACTGATGGAGTGCATCTTGTTAAAAGTCCCTTGTCTGTGCGAATTGATTTCCTTTGA

mRNA sequence

ATGGTGAACAGCGAAGCTCCTTTGATCGTTTCTGTTATGAGATTTTATGTTTTGTTTTCAATGTTTGCTTACAAATCCATGGCAGAAGAAGCAGACCAAAACCGCAAGGTTCACATCGTTTACCTTGGAGAAAGGCAATACGATGATATTAATTTAACAACTGAGTCTCACCATAATTTACTGGCCAAAGTATTGGGAAGTTATAGACATGGCTTTTCTGGGTTTGCAGCCAAGCTCACCAAGTCTCAGGCTCAAAAGCTAGTCGGTGTCATTGATACAGGATTTTGGCCGGAGTCCGCGTCTTTTGCAGACAAAGGAATGGGACCAGTACCATCACGATGGAAAGGCAAATGCAAATCAGGAGAATTGTTCAATTCCACAAACTGCAACAAGAAAGTCATAGGAGCACGCTGGTTCGTGAAGGGCTTCATCGCCGATTATGGGCGGGAGGCACTAGCCAACGAATGCTTATCCCCACGAGACACCCATGGACACGGAACCCACACAGCCAGCACAGCTGCAGGTTCATTTGTAACAAATGTTAGCTACCACGGCCTTGGTGCCGGTACGTTGAGGGGCGGTGCGCCACTCGCACGGTTGGCCATATACAAGGTAGGGTGGACCGGTGGTGGCGGGTCGATGGCGGACATATTGAAGGGCATAGATGTGGCCATTCACGACGGTGTGGATGTGCTGTCTATATCGATGGGGCTGCCACTTCCTTTGGTTCCAGAGTTGGACCAAGCAAATGGAATTGGCATTGGGTCATTTCATGCTATTGCGAGGGGGATTCCTGTTGTGTGTTCGGGTGGCAATGAAGGCCCTTATGAGCAGACGGTGGGAAACACTGCGCCTTGGATTTTGACTGTGGCTGCCTCTACAATGGATAGAGCCTTTCTTTCATCAATTACTCTTCTTGAAGATAATACCACTTATTTGGGCCAAAGCTTCTTCTATTCCAAAAAGGATCTTGTTGCTGAGTTGACCGTTCCGCCAGATAGAAGATGTGATCGCATTTCGGGAAATGAGAATTTTAGTGGGAAAGTTGTTCTCTGCTTCTCTGATAAAGTTAATCTGTCGGCGGCAACGGCGGTGAAAAAAGCAAATGGGAGTGGGATAATTGTCGCCGGCGAAAACATCGATATATTGTATGAGTTTGGCGAATCCTTTCCATGCGTCGTAGTGGATACCGATGTTGGCACAAAATTGTTCTTTCAAGCTTTAGACGACAGTAATCCAATGGTAAGGTTGAGGCATGCAAAAACCATCATCGGCAAGCCCATATCAACCAGGATTGCCCATTTCTCATCTAGAGGTCCAAATTCTGCTTCACCTGCAATTCTCAAGCCAGACATAGCCTCTCCTGGATCCAACATTCTAGCTGCCGTTCCTCCTGACTTTAGTTCAAACGACGAAGGGTTTGGCATGATGTCAGGAACTTCCATGGCTGCACCTCATGTCTCTGGCATCGTAGCTCTTCTTAAATCCCTACATCCTACTTGGTCACCTGCCGCCATTAAATCAGCCATCATCACCACGGCACGTGTAAAGAACCCCTCAGGATTGCCCATTTTTGCCAGCGGCAGTCCTCCCAAAGTCGCCGACCCATTTGACTACGGTGGTGGAGTGGTGGATCCCAACGCCGCCGCCGACCCAGGTCTGATCTACGATCTGGGCACCAGTGATTACATATATTATTACCTCTGCGCCATGGGTTATAAGGTTTCAGACATCTCTCACCTAACCAAGAAAAAAACAGAGTGTTCCTTGCAGAGACCTTCGATGTTGGACTTGAACCTGCCGACCATTACAGTCCCTGAACTCACAAACTCCACCACCGTGACTCGAACTGTGACCAATGTAGGGAACTTGACCTCCATTTATAGGGCAGTGGTCGAGCCTCCCCCTAGCACCAAGGTCAGTGTGGAGCCCCAGGTGTTGGCCTTCAACTCCAAAGTGAAAAAGATTTCCTTTAAGGTTACGATCTCCACCACCCTGTCTACGAACTACGGGTACTCTTTTGGAACCTTGACATGGACTGATGGAGTGCATCTTGTTAAAAGTCCCTTGTCTGTGCGAATTGATTTCCTTTGA

Coding sequence (CDS)

ATGGTGAACAGCGAAGCTCCTTTGATCGTTTCTGTTATGAGATTTTATGTTTTGTTTTCAATGTTTGCTTACAAATCCATGGCAGAAGAAGCAGACCAAAACCGCAAGGTTCACATCGTTTACCTTGGAGAAAGGCAATACGATGATATTAATTTAACAACTGAGTCTCACCATAATTTACTGGCCAAAGTATTGGGAAGTTATAGACATGGCTTTTCTGGGTTTGCAGCCAAGCTCACCAAGTCTCAGGCTCAAAAGCTAGTCGGTGTCATTGATACAGGATTTTGGCCGGAGTCCGCGTCTTTTGCAGACAAAGGAATGGGACCAGTACCATCACGATGGAAAGGCAAATGCAAATCAGGAGAATTGTTCAATTCCACAAACTGCAACAAGAAAGTCATAGGAGCACGCTGGTTCGTGAAGGGCTTCATCGCCGATTATGGGCGGGAGGCACTAGCCAACGAATGCTTATCCCCACGAGACACCCATGGACACGGAACCCACACAGCCAGCACAGCTGCAGGTTCATTTGTAACAAATGTTAGCTACCACGGCCTTGGTGCCGGTACGTTGAGGGGCGGTGCGCCACTCGCACGGTTGGCCATATACAAGGTAGGGTGGACCGGTGGTGGCGGGTCGATGGCGGACATATTGAAGGGCATAGATGTGGCCATTCACGACGGTGTGGATGTGCTGTCTATATCGATGGGGCTGCCACTTCCTTTGGTTCCAGAGTTGGACCAAGCAAATGGAATTGGCATTGGGTCATTTCATGCTATTGCGAGGGGGATTCCTGTTGTGTGTTCGGGTGGCAATGAAGGCCCTTATGAGCAGACGGTGGGAAACACTGCGCCTTGGATTTTGACTGTGGCTGCCTCTACAATGGATAGAGCCTTTCTTTCATCAATTACTCTTCTTGAAGATAATACCACTTATTTGGGCCAAAGCTTCTTCTATTCCAAAAAGGATCTTGTTGCTGAGTTGACCGTTCCGCCAGATAGAAGATGTGATCGCATTTCGGGAAATGAGAATTTTAGTGGGAAAGTTGTTCTCTGCTTCTCTGATAAAGTTAATCTGTCGGCGGCAACGGCGGTGAAAAAAGCAAATGGGAGTGGGATAATTGTCGCCGGCGAAAACATCGATATATTGTATGAGTTTGGCGAATCCTTTCCATGCGTCGTAGTGGATACCGATGTTGGCACAAAATTGTTCTTTCAAGCTTTAGACGACAGTAATCCAATGGTAAGGTTGAGGCATGCAAAAACCATCATCGGCAAGCCCATATCAACCAGGATTGCCCATTTCTCATCTAGAGGTCCAAATTCTGCTTCACCTGCAATTCTCAAGCCAGACATAGCCTCTCCTGGATCCAACATTCTAGCTGCCGTTCCTCCTGACTTTAGTTCAAACGACGAAGGGTTTGGCATGATGTCAGGAACTTCCATGGCTGCACCTCATGTCTCTGGCATCGTAGCTCTTCTTAAATCCCTACATCCTACTTGGTCACCTGCCGCCATTAAATCAGCCATCATCACCACGGCACGTGTAAAGAACCCCTCAGGATTGCCCATTTTTGCCAGCGGCAGTCCTCCCAAAGTCGCCGACCCATTTGACTACGGTGGTGGAGTGGTGGATCCCAACGCCGCCGCCGACCCAGGTCTGATCTACGATCTGGGCACCAGTGATTACATATATTATTACCTCTGCGCCATGGGTTATAAGGTTTCAGACATCTCTCACCTAACCAAGAAAAAAACAGAGTGTTCCTTGCAGAGACCTTCGATGTTGGACTTGAACCTGCCGACCATTACAGTCCCTGAACTCACAAACTCCACCACCGTGACTCGAACTGTGACCAATGTAGGGAACTTGACCTCCATTTATAGGGCAGTGGTCGAGCCTCCCCCTAGCACCAAGGTCAGTGTGGAGCCCCAGGTGTTGGCCTTCAACTCCAAAGTGAAAAAGATTTCCTTTAAGGTTACGATCTCCACCACCCTGTCTACGAACTACGGGTACTCTTTTGGAACCTTGACATGGACTGATGGAGTGCATCTTGTTAAAAGTCCCTTGTCTGTGCGAATTGATTTCCTTTGA

Protein sequence

MVNSEAPLIVSVMRFYVLFSMFAYKSMAEEADQNRKVHIVYLGERQYDDINLTTESHHNLLAKVLGSYRHGFSGFAAKLTKSQAQKLVGVIDTGFWPESASFADKGMGPVPSRWKGKCKSGELFNSTNCNKKVIGARWFVKGFIADYGREALANECLSPRDTHGHGTHTASTAAGSFVTNVSYHGLGAGTLRGGAPLARLAIYKVGWTGGGGSMADILKGIDVAIHDGVDVLSISMGLPLPLVPELDQANGIGIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMDRAFLSSITLLEDNTTYLGQSFFYSKKDLVAELTVPPDRRCDRISGNENFSGKVVLCFSDKVNLSAATAVKKANGSGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDDSNPMVRLRHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSNDEGFGMMSGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPPKVADPFDYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQRPSMLDLNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNSKVKKISFKVTISTTLSTNYGYSFGTLTWTDGVHLVKSPLSVRIDFL

Homology

[Close]

BLAST of Spg030021 vs. NCBI nr
Match: XP_023552781.1 (subtilisin-like protease SBT3.7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 883.6 bits (2282), Expect = 1.1e-252
Identity = 477/765 (62.35%), Postives = 558/765 (72.94%), Query Frame = 0


Query: 1   MVNSEAP---LIVSVMRFYVLFSMFAYKSMAEEADQNRKVHIVYLGERQYDDINLTTESH 60
           M N++AP   L+V+V+  Y +FS     +MAE  DQN KVHIVYLGER YDD+ LTT+SH
Sbjct: 1   MENNQAPSFMLVVAVVTIYGVFS-----AMAEADDQNSKVHIVYLGERPYDDVKLTTDSH 60

Query: 61  HNLLAKVLG-----------SYRHGFSGFAAKLTKSQAQKL------------------- 120
           H LL  VLG           SYRHGFSGFAAKLT SQAQKL                   
Sbjct: 61  HELLESVLGSKEKSLESIVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSPLYKMRT 120

Query: 121 ------------------------------VGVIDTGFWPESASFADKGMGPVPSRWKGK 180
                                         +GVIDTGFWPES SF DKGMGP+PSRWKG 
Sbjct: 121 TRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGI 180

Query: 181 CKSGELFNSTNCNKKVIGARWFVKGFIADYGREALANECLSPRDTHGHGTHTASTAAGSF 240
           C+SGE FNS++CNKKVIGARWF    +AD+G EA+  + LS RD  GHGTHTASTA G+F
Sbjct: 181 CESGEDFNSSHCNKKVIGARWFASALVADHGEEAVFMDYLSARDNQGHGTHTASTAGGAF 240

Query: 241 VTNVSYHGLGAGTLRGGAPLARLAIYKVGWTGGG-GSMADILKGIDVAIHDGVDVLSISM 300
           V NVS  G G GTLRGGAPLARLAIYKV W+    GS ADILKGID AIHDGVDVLS+S+
Sbjct: 241 VRNVSLFGNGRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSI 300

Query: 301 GLPLPLVPELDQANGIGIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMD 360
           G  +PL  ++++ N + +GSFHAIA+GI VVC+GGNEG  +QTV N APW+ TVAAST+D
Sbjct: 301 GKSIPLFSDVNELNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTID 360

Query: 361 RAFLSSITLLEDNTTYLGQSFFYSKKDLVAELTVPPDRRCDRISGNENFSGKVV-LCFSD 420
           RAFL+SIT L DN TYLGQ+F   KKD+V  L     R    +  N + SG VV LCF+D
Sbjct: 361 RAFLASITTLGDNATYLGQTFL--KKDIVGMLLAMDGRCAGLLGSNIHISGNVVLLCFTD 420

Query: 421 KVNLSAATAV----KKANGSGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDDS 480
               + A+ V    K+A   GII AG++ DIL       PC+ VDT VGTKLF   L+D 
Sbjct: 421 LAKKAGASNVVMPGKQAKVVGIIYAGQHNDILGPC--DTPCIHVDTHVGTKLFTYYLNDE 480

Query: 481 NPMVRLRHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSND 540
              +RLR  +TI GKPIS+RIA+FSSRGPNS SP ILKPDIA+PGSNI+AAVPP+   +D
Sbjct: 481 KAFIRLRATRTITGKPISSRIAYFSSRGPNSISPGILKPDIAAPGSNIIAAVPPNHKGSD 540

Query: 541 EGFGMMSGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPP 600
           +GF  MSGTSMAAPH+SGIVAL+KSL PTWSPAAIKSA+ITTARV++PSGLPIFA GSPP
Sbjct: 541 KGFAAMSGTSMAAPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPP 600

Query: 601 KVADPFDYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQ 660
           KVADPFDYGGGVVD NAA DPGLIYDLG +DYIYYYLC+MGY  ++ISHL+++KT C  +
Sbjct: 601 KVADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEAEISHLSQQKTVCPSK 660

Query: 661 RPSMLDLNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNS 697
           R S+LDLNLPTITVP LTNSTTVTRTVTNVGNLT++Y+AV++ PP +KV V+P+VLAFNS
Sbjct: 661 RASVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLAFNS 720

[Close]

BLAST of Spg030021 vs. NCBI nr
Match: XP_022984814.1 (subtilisin-like protease SBT3.9 [Cucurbita maxima])

HSP 1 Score: 882.9 bits (2280), Expect = 1.8e-252
Identity = 479/764 (62.70%), Postives = 555/764 (72.64%), Query Frame = 0


Query: 1   MVNSEAP-LIVSVMRFYVLFSMFAYKSMAEEADQNRKVHIVYLGERQYDDINLTTESHHN 60
           M N+ AP  I++V+  Y +FS      MAE  DQN KVHIVYLGER YDD+ LTT+SHH 
Sbjct: 1   MENNRAPSFILAVVTIYAVFS-----DMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHE 60

Query: 61  LLAKVLG-----------SYRHGFSGFAAKLTKSQAQKL--------------------- 120
           LL  VLG           SYRHGFSGFAAKLT SQAQKL                     
Sbjct: 61  LLESVLGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTR 120

Query: 121 ----------------------------VGVIDTGFWPESASFADKGMGPVPSRWKGKCK 180
                                       +GVIDTGFWPES SF DKGMGP+PSRWKG C+
Sbjct: 121 SWDFLGLSSSPSESSNLFHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQ 180

Query: 181 SGELFNSTNCNKKVIGARWFVKGFIADYGREALANECLSPRDTHGHGTHTASTAAGSFVT 240
           SGE FNS++CNKKVIGARWF    IAD+G EA+  + LS RD  GHGTHTASTA G+FV 
Sbjct: 181 SGEDFNSSHCNKKVIGARWFASALIADHGEEAVFKDYLSARDNEGHGTHTASTAGGAFVR 240

Query: 241 NVSYHGLGAGTLRGGAPLARLAIYKVGWTGGG-GSMADILKGIDVAIHDGVDVLSISMGL 300
           NVSY G G GTLRGGAPLARLAIYKV W+    GS ADILKGID AIHDGVDVLS+S+G 
Sbjct: 241 NVSYFGNGRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIGK 300

Query: 301 PLPLVPELDQANGIGIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMDRA 360
            +PL P+++  N + +GSFHAIA+GI VVC+GGNEG  +QTV N APW+ TVAAST+DRA
Sbjct: 301 SIPLFPDVNDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRA 360

Query: 361 FLSSITLLEDNTTYLGQSFFYSKKDLVAELTVPPDRRCDRISGNE-NFSGKVV-LCFSDK 420
           FL SIT L DN TYLGQ F   +KD+V  L V  D RC  I G +   SG VV LCF+D 
Sbjct: 361 FLVSITTLGDNATYLGQMFL--RKDIVGML-VAMDGRCAGILGGDIPISGNVVLLCFTDL 420

Query: 421 VNLS-AATAV---KKANGSGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDDSN 480
              + A+ AV   K+A   G+I AG+  D L       PC+ VDT VGTKLF   L+D +
Sbjct: 421 AKTAPASNAVMPGKQAKVVGVIYAGQQTDNLVPC--DIPCIHVDTHVGTKLFTYLLNDDD 480

Query: 481 PMVRLRHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSNDE 540
            ++RLR  +TIIGKPIS+RIA+FSSRGPNS SP ILKPDIA+PG+NI+AAVPP+    D+
Sbjct: 481 ALIRLRATRTIIGKPISSRIAYFSSRGPNSFSPGILKPDIAAPGANIIAAVPPNHKGGDK 540

Query: 541 GFGMMSGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPPK 600
           GF  MSGTSMA PH+SGIV L+KSLHPTWSPAAIKSA+ITTARV++PSG+PIFA GSPPK
Sbjct: 541 GFATMSGTSMATPHISGIVTLIKSLHPTWSPAAIKSALITTARVEDPSGMPIFAEGSPPK 600

Query: 601 VADPFDYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQR 660
           VADPFDYGGGVVD NAA DPGLIYDLG +DYIYYYLC+MGY  +DISHL+++KT C  +R
Sbjct: 601 VADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKR 660

Query: 661 PSMLDLNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNSK 697
            S+LDLNLPTITVP L NSTTVTRTVTNVGNLT++Y+AV++ PP +KV V+P+VL FNS 
Sbjct: 661 ASILDLNLPTITVPALINSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSN 720

[Close]

BLAST of Spg030021 vs. NCBI nr
Match: XP_008452040.1 (PREDICTED: subtilisin-like protease SBT3.7 isoform X1 [Cucumis melo])

HSP 1 Score: 877.5 bits (2266), Expect = 7.8e-251
Identity = 475/764 (62.17%), Postives = 545/764 (71.34%), Query Frame = 0


Query: 2   VNSE-APLIVSVMRFYVLFSMFAYKSMAEEADQNRKVHIVYLGERQYDDINLTTESHHNL 61
           +NS+ A LIV  + FY L        +AE  DQN KVHIVYLGE+ + D   TT+SHH L
Sbjct: 3   INSQLAHLIVGFIIFYGLCEPI----LAESNDQNPKVHIVYLGEKPHHDTKFTTDSHHQL 62

Query: 62  LAKVLG-----------SYRHGFSGFAAKLTKSQAQKL---------------------- 121
           LA +LG           SY+HGFSGFAAKLTKS+AQKL                      
Sbjct: 63  LAAILGSKEKSLEAMVYSYKHGFSGFAAKLTKSEAQKLSEMSRVVRVVPSSLYKVHTTRS 122

Query: 122 --------------------------VGVIDTGFWPESASFADKGMGPVPSRWKGKCKSG 181
                                     +GVID+G WPES SF DKG+GP+PSRWKG C+SG
Sbjct: 123 WDFLGLSSSPSESSNLLHRAKGDDVIIGVIDSGIWPESESFKDKGLGPIPSRWKGTCESG 182

Query: 182 ELFNSTNCNKKVIGARWFVKGFIADYGREALANECLSPRDTHGHGTHTASTAAGSFVTNV 241
           E FNSTNCNKK+IGARWFVK F+ADYGREALA E LSPRD HGHGTHTASTAAGSFV N+
Sbjct: 183 EQFNSTNCNKKIIGARWFVKAFVADYGREALAKEYLSPRDLHGHGTHTASTAAGSFVANI 242

Query: 242 SYHGLGAGTLRGGAPLARLAIYKVGWTGGG-GSMADILKGIDVAIHDGVDVLSISMGLPL 301
           +YH   AGT RGGAPLARLAIYK  WT  G GS ADILK ID AIHDGVDVLSIS+G   
Sbjct: 243 NYHNNAAGTARGGAPLARLAIYKALWTNRGVGSSADILKAIDEAIHDGVDVLSISIGGSP 302

Query: 302 PLVPELDQANGIGIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMDRAFL 361
           P  PE  + + I  GSFHAI +GI VVC+ GN GP  Q V N APWI TVA +T+DRAFL
Sbjct: 303 PFYPEFTELSDIAFGSFHAITKGISVVCAAGNSGPSPQMVDNVAPWIFTVATNTIDRAFL 362

Query: 362 SSITLLEDNTTYLGQSFFYSKKDLVAELTVPPDRRCDRISGNENF-SGKVVLCFSDKVNL 421
           SSIT L DNTT++GQS   SKKDLVAEL      RCD++SGNE F +GKVVLCF    + 
Sbjct: 363 SSITTLRDNTTFMGQSLLESKKDLVAELV--SWERCDQLSGNEAFINGKVVLCFPKLADY 422

Query: 422 S----AATAVKKANGSGIIVAGENID--ILYEFGESFPCVVVDTDVGTKLFFQALDDS-N 481
           S    AA  V +ANG+GIIVAG+ +D  +L       PC++VDT VG+KLFF  L +S +
Sbjct: 423 STISKAAEVVVRANGTGIIVAGQQVDNNLLACISSPIPCILVDTIVGSKLFFYFLQNSDH 482

Query: 482 PMVRLRHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSNDE 541
           P+V LR A+TIIGKPI+  IAHFSSRGPNS SP ILKPDI++PGSNIL+AV P +  N++
Sbjct: 483 PVVMLRAARTIIGKPIAPTIAHFSSRGPNSVSPPILKPDISAPGSNILSAVSPHYFFNEK 542

Query: 542 GFGMMSGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPPK 601
           GF +MSGTSMA PHVS IVALLKS+HPTWSPAAIKSA++TTAR +   GLPIFA G+PPK
Sbjct: 543 GFSLMSGTSMATPHVSAIVALLKSVHPTWSPAAIKSALMTTARTEVSPGLPIFADGTPPK 602

Query: 602 VADPFDYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQR 661
           VADPFDYG GVVD NAA DPGLIYD+G  DYI YYLC MGY+  DISHLT +KTEC LQR
Sbjct: 603 VADPFDYGAGVVDANAAVDPGLIYDMGRKDYIDYYLCGMGYEDKDISHLTHRKTECPLQR 662

Query: 662 PSMLDLNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNSK 697
            S+LDLNLP IT+P L NST VTRTVTNVGNL+ +Y+A +E P   KVSV PQVL FNS+
Sbjct: 663 LSLLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAKIEAPFGCKVSVNPQVLVFNSQ 722

[Close]

BLAST of Spg030021 vs. NCBI nr
Match: KAG6577195.1 (Subtilisin-like protease 3.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 876.3 bits (2263), Expect = 1.7e-250
Identity = 473/765 (61.83%), Postives = 556/765 (72.68%), Query Frame = 0


Query: 1   MVNSEAP---LIVSVMRFYVLFSMFAYKSMAEEADQNRKVHIVYLGERQYDDINLTTESH 60
           M N++AP   L+V+V+  YV+FS     +MAE  DQN KVHIVYLGER YDD+ LTT+SH
Sbjct: 1   MENNQAPSFMLVVAVVTIYVVFS-----AMAEADDQNSKVHIVYLGERPYDDVKLTTDSH 60

Query: 61  HNLLAKVLG-----------SYRHGFSGFAAKLTKSQAQKL------------------- 120
           H LL  V+G           SY+HGFSGFAAKLT SQAQKL                   
Sbjct: 61  HELLESVMGSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSPLYKMRT 120

Query: 121 ------------------------------VGVIDTGFWPESASFADKGMGPVPSRWKGK 180
                                         +GVIDTGFWPES SF DKGMGP+PSRWKG 
Sbjct: 121 TRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGI 180

Query: 181 CKSGELFNSTNCNKKVIGARWFVKGFIADYGREALANECLSPRDTHGHGTHTASTAAGSF 240
           C+SGE FNS++CNKKVIGARWF    +AD+G EA+  + LS RD  GHGTHTASTA G+F
Sbjct: 181 CQSGEDFNSSHCNKKVIGARWFASALVADHGEEAVFMDYLSARDNEGHGTHTASTAGGAF 240

Query: 241 VTNVSYHGLGAGTLRGGAPLARLAIYKVGWTGGG-GSMADILKGIDVAIHDGVDVLSISM 300
           V NVS+ G G GTLRGGAPLARLAIYKV W+    GS ADILKGID AIHDGVDVLS+S+
Sbjct: 241 VRNVSFFGNGRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSI 300

Query: 301 GLPLPLVPELDQANGIGIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMD 360
           G  +PL  ++++ N + +GSFHAIA+GI VVCSGGNEG  +QTV N APW+ TVAAST+D
Sbjct: 301 GKSIPLFSDVNELNPVAVGSFHAIAKGISVVCSGGNEGSIQQTVENVAPWLFTVAASTID 360

Query: 361 RAFLSSITLLEDNTTYLGQSFFYSKKDLVAELTVPPDRRCDRISGNENFSGKVV-LCFSD 420
           RAFL+SIT L DN TYLGQ+F   KKD+V  L     R    +  N + SG VV LCF+D
Sbjct: 361 RAFLASITTLGDNATYLGQTFL--KKDIVGMLLAMDGRCAGLLGSNIHISGNVVLLCFTD 420

Query: 421 KVNLSAATAV----KKANGSGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDDS 480
               + A+ V    K+A   GII AG++ DIL       PC+ VDT VGTKLF   L+D 
Sbjct: 421 LAKKAGASNVVMPGKQAKVVGIIYAGQHNDILGPC--DTPCIHVDTHVGTKLFTYYLNDE 480

Query: 481 NPMVRLRHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSND 540
              +R+R  +TI GKPIS+RIA+FSSRGPNS SP ILKPDIA+PG+NI+AAVPP+    D
Sbjct: 481 KAFIRVRATRTITGKPISSRIAYFSSRGPNSISPGILKPDIAAPGANIIAAVPPNHEGGD 540

Query: 541 EGFGMMSGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPP 600
           +GF +MSGTSMAAPH+SGIVAL+KSL PTWSPAAIKSA+ITTARV++ SGLPIFA GSPP
Sbjct: 541 KGFAVMSGTSMAAPHISGIVALIKSLRPTWSPAAIKSALITTARVEDLSGLPIFAEGSPP 600

Query: 601 KVADPFDYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQ 660
           KVAD FDYGGGVVD NAA DPGLIYDL  +DYIYYYLC+MGY  +DISHL+++KT C  +
Sbjct: 601 KVADSFDYGGGVVDANAAIDPGLIYDLEVTDYIYYYLCSMGYTEADISHLSQQKTVCPSK 660

Query: 661 RPSMLDLNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNS 697
           R S+LDLNLPTITVP LTNSTTVTRTVTNVGNLT++Y+AV++ PP +KV V+P+VL FNS
Sbjct: 661 RTSILDLNLPTITVPTLTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNS 720

[Close]

BLAST of Spg030021 vs. NCBI nr
Match: XP_022931500.1 (subtilisin-like protease SBT3.7 [Cucurbita moschata])

HSP 1 Score: 875.9 bits (2262), Expect = 2.3e-250
Identity = 476/766 (62.14%), Postives = 558/766 (72.85%), Query Frame = 0


Query: 1   MVNSEAP---LIVSVMRFYVLFSMFAYKSMAEEADQNRKVHIVYLGERQYDDINLTTESH 60
           M N++AP   L+V+V+  YV+FS     +M E  DQN KVHIVYLGER YDD+ LTT+SH
Sbjct: 1   MENNQAPSFMLVVAVVTIYVVFS-----AMGEADDQNSKVHIVYLGERPYDDVTLTTDSH 60

Query: 61  HNLLAKVLG-----------SYRHGFSGFAAKLTKSQAQKL------------------- 120
           H LL  VLG           SYRHGFSGFAAKLT SQAQKL                   
Sbjct: 61  HELLESVLGSKEKSLESIVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSPLYKMRT 120

Query: 121 ------------------------------VGVIDTGFWPESASFADKGMGPVPSRWKGK 180
                                         +GVIDTGFWPES SF DKGMGP+PSRWKG 
Sbjct: 121 TRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGI 180

Query: 181 CKSGELFNSTNCNKKVIGARWFVKGFIADYGREALANECLSPRDTHGHGTHTASTAAGSF 240
           C+SGE FNS++CNKKVIGARWF    +AD+G EA+  + LS RD  GHGTHTASTA G+F
Sbjct: 181 CQSGEDFNSSHCNKKVIGARWFASALVADHGEEAVFMDYLSARDNEGHGTHTASTAGGAF 240

Query: 241 VTNVSYHGLGAGTLRGGAPLARLAIYKVGWTGGG-GSMADILKGIDVAIHDGVDVLSISM 300
           V NVSY G G GTLRGGAPLARLAIYKV W+    GS ADILKGID AIHDGVDVLS+S+
Sbjct: 241 VRNVSYFGNGRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSI 300

Query: 301 GLPLPLVPELDQANGIGIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMD 360
           G  +PL  ++++ N + +GSFHAIA+GI VVC+GGNEG  +QTV N APW+ TVAA+T+D
Sbjct: 301 GKSIPLFSDVNELNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTID 360

Query: 361 RAFLSSITLLEDNTTYLGQSFFYSKKDLVAELTVPPDRRCDRISG-NENFSGKVV-LCFS 420
           RAFL+SIT L DN TYLGQ+F   KKD+V  L +  DRRC  + G N   SG VV LCF+
Sbjct: 361 RAFLASITTLGDNATYLGQTFL--KKDIVGML-LAMDRRCAGLLGSNIPISGNVVLLCFT 420

Query: 421 DKVNLSAATAV----KKANGSGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDD 480
           D    + A+ V    K A   GII AG++ DIL       PC+ VDT VGT+LF   L+D
Sbjct: 421 DLAKKAGASNVVMPGKPAKVVGIIYAGQHNDILGPC--DTPCIHVDTHVGTQLFTYYLND 480

Query: 481 SNPMVRLRHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSN 540
               +R+R  +TI GKPIS+RIA+FSSRGPNS SP ILKPDIA+PG+NI+AAVPP+    
Sbjct: 481 EKAFIRVRATRTITGKPISSRIAYFSSRGPNSISPGILKPDIAAPGANIIAAVPPNHEGG 540

Query: 541 DEGFGMMSGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSP 600
           D+GF +MSGTSMAAPH+SGIVAL+KSL PTWSPA IKSA+ITTARV++ SGLPIFA GSP
Sbjct: 541 DKGFAVMSGTSMAAPHISGIVALIKSLRPTWSPAVIKSALITTARVEDLSGLPIFAEGSP 600

Query: 601 PKVADPFDYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSL 660
           PKVADPFDYGGGVVD NAA DPGLIYDL  +DYIYYYLC+MGY  +DISHL+++KT C  
Sbjct: 601 PKVADPFDYGGGVVDANAAIDPGLIYDLEVTDYIYYYLCSMGYTEADISHLSQQKTVCPS 660

Query: 661 QRPSMLDLNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFN 697
           +R S+LDLNLPTITVP LTNSTTVTRTVTNVGNLT++Y+AV++ PP +KV V+P+VL FN
Sbjct: 661 KRTSILDLNLPTITVPTLTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFN 720

[Close]

BLAST of Spg030021 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 1.0e-192
Identity = 374/766 (48.83%), Postives = 484/766 (63.19%), Query Frame = 0


Query: 16  YVLFSMFAY----KSMAEEADQNRKVHIVYLGERQYDDINLTTESHHNLLAKVLG----- 75
           YV+ S+  +    +S   E+   RKVHIVYLGE+Q+DD    TESHH +L  +LG     
Sbjct: 9   YVVLSLVIFLNVQRSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDA 68

Query: 76  ------SYRHGFSGFAAKLTKSQAQK---------------------------------- 135
                 SYRHGFSGFAAKLT+SQA+K                                  
Sbjct: 69  NDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANP 128

Query: 136 -------------LVGVIDTGFWPESASFADKGMGPVPSRWKGKCKSGELFNSTNCNKKV 195
                        ++GVIDTG WPES  F D G GPVPS WKG C++GE FNS+NCNKK+
Sbjct: 129 KSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKL 188

Query: 196 IGARWFVKGFIADYGREALAN--ECLSPRDTHGHGTHTASTAAGSFVTNVSYHGLGAGTL 255
           IGA++F+ GF+A+       N  + +SPRD  GHGTH ++ A GSFV N+SY GL  GT+
Sbjct: 189 IGAKYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTV 248

Query: 256 RGGAPLARLAIYKVGWTGGGG-----SMADILKGIDVAIHDGVDVLSISMGLPLPLVPEL 315
           RGGAP A +A+YK  W          S ADILK +D A+HDGVDVLSIS+G  +PL  E 
Sbjct: 249 RGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGET 308

Query: 316 DQANGIGIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMDRAFLSSITLL 375
           D  +GI  G+FHA+ +GI VVCSGGN GP   TV NTAPWI+TVAA+T+DR+F + +T L
Sbjct: 309 DIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLT-L 368

Query: 376 EDNTTYLGQSFFYSKKDLVAELTVP--PDRRCDRISG---------NENFSGKVVLCFSD 435
            +N   LGQ+ +         L  P  P    +  SG         N    GKVVLCF+ 
Sbjct: 369 GNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVVLCFTT 428

Query: 436 K----VNLSAATAVKKANGSGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDDS 495
                  LSAA  VK+A G G+I+A      +    + FPCV VD ++GT +        
Sbjct: 429 SPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSG 488

Query: 496 NPMVRLRHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSND 555
           +P+V+++ +KT++G+P+ T++A FSSRGPNS +PAILKPDIA+PG +ILAA   + + +D
Sbjct: 489 SPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAAT-TNTTFSD 548

Query: 556 EGFGMMSGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPP 615
           +GF M+SGTSMAAP +SG+ ALLK+LH  WSPAAI+SAI+TTA   +P G  IFA GSPP
Sbjct: 549 QGFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPP 608

Query: 616 KVADPFDYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQ 675
           K+ADPFDYGGG+V+P  +A+PGL+YD+G  DY+  Y+C++GY  + IS L  K T CS  
Sbjct: 609 KLADPFDYGGGLVNPEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQLIGKTTVCSNP 668

Query: 676 RPSMLDLNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNS 698
           +PS+LD NLP+IT+P L +  T+TRTVTNVG L S+YR  VEPP   +V+V P+ L FNS
Sbjct: 669 KPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNS 728

[Close]

BLAST of Spg030021 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 3.2e-191
Identity = 378/760 (49.74%), Postives = 482/760 (63.42%), Query Frame = 0


Query: 17  VLFSMFAYKSMAEEADQNRKVHIVYLGERQYDDINLTTESHHNLLAKVLG---------- 76
           V+ S+    ++A  A    KVHIVYLGE+Q+DD    TESHH +L+ +LG          
Sbjct: 10  VVLSLIIVLNVA-RASAKSKVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMV 69

Query: 77  -SYRHGFSGFAAKLTKSQAQK--------------------------------------- 136
            SYRHGFSGFAAKLTKSQA+K                                       
Sbjct: 70  YSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYELATTRIWDYLGPSADNSKNLVS 129

Query: 137 --------LVGVIDTGFWPESASFADKGMGPVPSRWKGKCKSGELFNSTNCNKKVIGARW 196
                   ++GVIDTG WPES SF D G+GPVPS WKG C+ GE F STNCN+K+IGA++
Sbjct: 130 DTNMGDQTIIGVIDTGVWPESESFNDYGVGPVPSHWKGGCEPGENFISTNCNRKLIGAKY 189

Query: 197 FVKGFIADYGREALAN-ECLSPRDTHGHGTHTASTAAGSFVTNVSYHGLGAGTLRGGAPL 256
           F+ GF+A+    A  + + +S RD  GHGTH AS A GSFV NVSY GLG GTLRGGAP 
Sbjct: 190 FINGFLAENQFNATESPDYISARDFDGHGTHVASIAGGSFVPNVSYKGLGRGTLRGGAPR 249

Query: 257 ARLAIYKVGW-----TGGGGSMADILKGIDVAIHDGVDVLSISMGLPLPLVPELDQANGI 316
           AR+A+YK  W      G   S +DI+K ID AIHDGVDVLSIS+G  +PL  E D  +GI
Sbjct: 250 ARIAMYKACWYINELDGVTCSFSDIMKAIDEAIHDGVDVLSISLGGRVPLNSETDLRDGI 309

Query: 317 GIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMDRAFLSSITLLEDNTTY 376
             G+FHA+A+GI VVC+GGN GP  QTV NTAPWILTVAA+T+DR+F + I +L +N   
Sbjct: 310 ATGAFHAVAKGIVVVCAGGNAGPSSQTVVNTAPWILTVAATTLDRSFATPI-ILGNNQVI 369

Query: 377 LGQSFFYSKKDLVAELTVP--PDRRCDRISG---------NENFSGKVVLCFSD----KV 436
           LGQ+ +   +     L  P  P    D  SG         N   +GKVVLCF+      V
Sbjct: 370 LGQAMYIGPELGFTSLVYPEDPGNSIDTFSGVCESLNLNSNRTMAGKVVLCFTTARDFTV 429

Query: 437 NLSAATAVKKANGSGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDDSNPMVRL 496
             +AA+ VK A G G+I+A      L    + FPCV +D ++GT + F      +P+V++
Sbjct: 430 VSTAASIVKAAGGLGLIIARNPGYNLAPCSDDFPCVAIDNELGTDILFYIRYTGSPVVKI 489

Query: 497 RHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSNDEGFGMM 556
           + ++T++G+P+ T++A FSSRGPNS SPAILKPDIA+PG +ILAA  P+ + N  GF M 
Sbjct: 490 QPSRTLVGEPVGTKVATFSSRGPNSISPAILKPDIAAPGVSILAATSPNDTLNAGGFVMR 549

Query: 557 SGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPPKVADPF 616
           SGTSMAAP +SG++ALLKSLHP WSPAA +SAI+TTA   +P G  I A  S  KV DPF
Sbjct: 550 SGTSMAAPVISGVIALLKSLHPDWSPAAFRSAIVTTAWRTDPFGEQIAAESSSLKVPDPF 609

Query: 617 DYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQRPSMLD 676
           DYGGG+V+P  AA+PGLI D+ + DY+  YLC+ GY  S IS L  K T CS  +PS+LD
Sbjct: 610 DYGGGLVNPEKAAEPGLILDMDSQDYV-LYLCSAGYNDSSISRLVGKVTVCSNPKPSVLD 669

Query: 677 LNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNSKVKKIS 698
           +NLP+IT+P L +  T+TRTVTNVG + S+Y+ +VEPP   +V V P+ L FNSK K +S
Sbjct: 670 INLPSITIPNLKDEVTLTRTVTNVGPVDSVYKVLVEPPLGIQVVVTPETLVFNSKTKSVS 729

[Close]

BLAST of Spg030021 vs. ExPASy Swiss-Prot
Match: Q9SZY2 (Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 SV=2)

HSP 1 Score: 668.3 bits (1723), Expect = 9.4e-191
Identity = 365/746 (48.93%), Postives = 482/746 (64.61%), Query Frame = 0


Query: 31  ADQNRKVHIVYLGERQYDDINLTTESHHNLLAKVLG-----------SYRHGFSGFAAKL 90
           A    KVHIVYLGE+Q+DD    TESHH +L  +LG           S+RHGFSGFAAKL
Sbjct: 28  AGAESKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEEAHGSMVHSFRHGFSGFAAKL 87

Query: 91  TKSQAQK-----------------------------------------------LVGVID 150
           T+SQA+K                                               ++G+ID
Sbjct: 88  TESQAKKIADLPEVVHVIPDRFYKPATTRTWDYLGLSPTNPKNLLNQTNMGEQMIIGIID 147

Query: 151 TGFWPESASFADKGMGPVPSRWKGKCKSGELFNSTNCNKKVIGARWFVKGFIADYG--RE 210
           +G WPES  F D  +GPVPS WKG C+SGE FNS++CNKK+IGA++F+  F+A +     
Sbjct: 148 SGVWPESEVFNDNEIGPVPSHWKGGCESGEDFNSSHCNKKLIGAKYFINAFLATHESFNS 207

Query: 211 ALANECLSPRDTHGHGTHTASTAAGSFVTNVSYHGLGAGTLRGGAPLARLAIYKVGW--- 270
           + + + +SPR  +GHGTH A+ A GS+V N SY GL  GT+RGGAP AR+A+YK  W   
Sbjct: 208 SESLDFISPRGYNGHGTHVATIAGGSYVPNTSYKGLAGGTVRGGAPRARIAVYKTCWYLD 267

Query: 271 -TGGGGSMADILKGIDVAIHDGVDVLSISMGLPLPLVPELDQANGIGIGSFHAIARGIPV 330
                 S ADILK +D AIHDGVDVLS+S+G   PL PE D  +GI  G+FHA+ +GI V
Sbjct: 268 LDIAACSSADILKAMDEAIHDGVDVLSLSLGFE-PLYPETDVRDGIATGAFHAVLKGITV 327

Query: 331 VCSGGNEGPYEQTVGNTAPWILTVAASTMDRAFLSSITLLEDNTTYLGQSFFYSKKDLVA 390
           VC+ GN GP  QTVGNTAPWILTVAA+T+DR+F++ +T L +N   LGQ+ +   +    
Sbjct: 328 VCAAGNAGPAAQTVGNTAPWILTVAATTLDRSFVTPMT-LGNNKVILGQAIYTGTEVGFT 387

Query: 391 ELTVPPD---------RRCDR--ISGNENFSGKVVLCFSD---KVNLS-AATAVKKANGS 450
            L  P +           C+R  I+ N   +GKVVLCF++    ++++ AA  VK+A G 
Sbjct: 388 SLVYPENPGNSNESFSGTCERLLINSNRTMAGKVVLCFTESPYSISVTRAAHYVKRAGGL 447

Query: 451 GIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDDSNPMVRLRHAKTIIGKPISTR 510
           G+I+AG+  ++L    + FPCV VD ++GT + F    + +P+V+++ ++T+IG+P+ T+
Sbjct: 448 GVIIAGQPGNVLRPCLDDFPCVAVDYELGTYILFYIRSNGSPVVKIQPSRTLIGQPVGTK 507

Query: 511 IAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSNDEGFGMMSGTSMAAPHVSGIV 570
           +A FSSRGPN  S AILKPDIA+PG +ILAA   + + ND GF  +SGTSMA P +SGIV
Sbjct: 508 VASFSSRGPNPISAAILKPDIAAPGVSILAATTTNTTFNDRGFIFLSGTSMATPTISGIV 567

Query: 571 ALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPPKVADPFDYGGGVVDPNAAAD 630
           ALLK+LHP WSPAAI+SAI+TTA   +P G  IFA GSP K ADPFDYGGG+V+P  A  
Sbjct: 568 ALLKALHPDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKPADPFDYGGGLVNPEKATK 627

Query: 631 PGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQRPSMLDLNLPTITVPELTNS 690
           PGL+YDLG  DY+  Y+C++GY  + IS L  K T CS  +PS+LD NLP+IT+P L   
Sbjct: 628 PGLVYDLGLEDYV-LYMCSVGYNETSISQLVGKGTVCSYPKPSVLDFNLPSITIPNLKEE 687

Query: 691 TTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNSKVKKISFKVTISTTLSTNYG 698
            T+ RT+TNVG L S+YR  VEPP  T+V+V P+ L FNS  K++SFKV++STT   N G
Sbjct: 688 VTLPRTLTNVGPLESVYRVAVEPPLGTQVTVTPETLVFNSTTKRVSFKVSVSTTHKINTG 747

[Close]

BLAST of Spg030021 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 666.8 bits (1719), Expect = 2.7e-190
Identity = 371/764 (48.56%), Postives = 476/764 (62.30%), Query Frame = 0


Query: 16  YVLFSMFAYKSMAEEADQNRKVHIVYLGERQYDDINLTTESHHNLLAKVLG--------- 75
           +V   +    +    A    KVHIVYLGE+Q+DD    TESHH +L  +LG         
Sbjct: 9   FVAIILNGLSTFVAHAGAESKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDAHSSM 68

Query: 76  --SYRHGFSGFAAKLTKSQAQKL------------------------------------- 135
             SYRHGFSGFAAKLTKSQA+KL                                     
Sbjct: 69  VHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDYLGLSVANPKNLL 128

Query: 136 ----------VGVIDTGFWPESASFADKGMGPVPSRWKGKCKSGELFNSTNCNKKVIGAR 195
                     +G++D+G WPES  F D G+GPVPS WKG C SGE F S+ CNKK+IGA+
Sbjct: 129 NDTNMGEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENFTSSQCNKKLIGAK 188

Query: 196 WFVKGFIADY----GREALANECLSPRDTHGHGTHTASTAAGSFVTNVSYHGLGAGTLRG 255
           +F+ GF+A +      E+L  + +SPRD  GHGTH A+ A GS+V ++SY GL  GT+RG
Sbjct: 189 YFINGFLATHESFNSTESL--DFISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRG 248

Query: 256 GAPLARLAIYKVGW-----TGGGGSMADILKGIDVAIHDGVDVLSISMGLPLPLVPELDQ 315
           GAP AR+A+YK  W          S ADILK +D A+HDGVDVLS+S+G   P  PE D 
Sbjct: 249 GAPRARIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSIGYRFPYFPETDV 308

Query: 316 ANGIGIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMDRAFLSSITLLED 375
              I  G+FHA+ +GI VVCSGGN GP  QTVGNTAPWILTVAA+T+DR+F + IT L +
Sbjct: 309 RAVIATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPIT-LGN 368

Query: 376 NTTYLGQSFFYSKKDLVAELTVP--PDRRCDRISG---------NENFSGKVVLCFSDKV 435
           N   LGQ+ +   +     L  P  P    +  SG         N   +GKVVLCF+   
Sbjct: 369 NKLILGQAMYTGPELGFTSLVYPENPGNSNESFSGDCELLFFNSNHTMAGKVVLCFTTST 428

Query: 436 NL----SAATAVKKANGSGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDDSNP 495
                 SA + VK+A G G+IVA    D L    + FPCV VD ++GT +         P
Sbjct: 429 RYITVSSAVSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGTDILLYIRSTGLP 488

Query: 496 MVRLRHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSNDEG 555
           +V+++ +KT++G+P+ T++A FSSRGPNS  PAILKPDIA+PG +ILAA   + + ND G
Sbjct: 489 VVKIQPSKTLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILAATTTNKTFNDRG 548

Query: 556 FGMMSGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPPKV 615
           F  +SGTSMAAP +SG+VALLK+LH  WSPAAI+SAI+TTA   +P G  IFA GSP K+
Sbjct: 549 FIFLSGTSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKL 608

Query: 616 ADPFDYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQRP 675
           ADPFDYGGG+V+P  AA PGL+YDLG  DY+  Y+C++GY  + IS L  K T CS  +P
Sbjct: 609 ADPFDYGGGLVNPEKAAKPGLVYDLGLEDYV-LYMCSVGYNETSISQLVGKGTVCSNPKP 668

Query: 676 SMLDLNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNSKV 698
           S+LD NLP+IT+P L +  T+TRT+TNVG L S+Y+ V+EPP   +V+V P+ L FNS  
Sbjct: 669 SVLDFNLPSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVTPETLLFNSTT 728

[Close]

BLAST of Spg030021 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 3.9e-189
Identity = 365/747 (48.86%), Postives = 475/747 (63.59%), Query Frame = 0


Query: 31  ADQNRKVHIVYLGERQYDDINLTTESHHNLLAKVLG-----------SYRHGFSGFAAKL 90
           A    KVHIVYLGE+Q+DD    +ESHH +L+ +LG           SYRHGFSGFAAKL
Sbjct: 23  ASDESKVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAHESMVYSYRHGFSGFAAKL 82

Query: 91  TKSQAQKL-----------------------------------------------VGVID 150
           T+SQA+KL                                               +G ID
Sbjct: 83  TESQAKKLADSPEVVHVMADSFYELATTRTWDYLGLSVANPNNLLNDTNMGDQVIIGFID 142

Query: 151 TGFWPESASFADKGMGPVPSRWKGKCKSGELFNSTNCNKKVIGARWFVKGFIADYG--RE 210
           TG WPES SF D G+GP+PS WKG C+SGE F STNCN+K+IGA++F+ GF+A+      
Sbjct: 143 TGVWPESESFNDNGVGPIPSHWKGGCESGEKFISTNCNRKLIGAKYFINGFLAENEGFNT 202

Query: 211 ALANECLSPRDTHGHGTHTASTAAGSFVTNVSYHGLGAGTLRGGAPLARLAIYKVGW--- 270
             + + +S RD  GHGTHTAS A GSFV N+SY GL  G LRGGAP AR+AIYK  W   
Sbjct: 203 TESRDYISARDFIGHGTHTASIAGGSFVPNISYKGLAGGNLRGGAPRARIAIYKACWYVD 262

Query: 271 --TGGGGSMADILKGIDVAIHDGVDVLSISMGLPLPLVPELDQANGIGIGSFHAIARGIP 330
                  S +DILK +D ++HDGVDVLS+S+G  +PL PE D  + I  G+FHA+A+GI 
Sbjct: 263 QLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPETDLRDRIATGAFHAVAKGII 322

Query: 331 VVCSGGNEGPYEQTVGNTAPWILTVAASTMDRAFLSSITLLEDNTTYLGQSFFYSKKDLV 390
           VVC+GGN GP  QTV NTAPWI+TVAA+T+DR+F + IT L +    LGQ+ +  ++   
Sbjct: 323 VVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPIT-LGNRKVILGQALYTGQELGF 382

Query: 391 AELTVPPDRR---------CDRISGNEN--FSGKVVLCFSDKVNLS----AATAVKKANG 450
             L  P +           C+R++ N N   +GKVVLCF+     +    AA+ VK A G
Sbjct: 383 TSLVYPENAGFTNETFSGVCERLNLNPNRTMAGKVVLCFTTNTLFTAVSRAASYVKAAGG 442

Query: 451 SGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDDSNPMVRLRHAKTIIGKPIST 510
            G+I+A      L    + FPCV +D ++GT +        +P+V+++ ++T++G+P+ T
Sbjct: 443 LGVIIARNPGYNLTPCRDDFPCVAIDYELGTDVLLYIRSTRSPVVKIQPSRTLVGQPVGT 502

Query: 511 RIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSNDEGFGMMSGTSMAAPHVSGI 570
           ++A FSSRGPNS SPAILKPDI +PG +ILAA  PD +S+  GF +++GTSMAAP V+G+
Sbjct: 503 KVATFSSRGPNSISPAILKPDIGAPGVSILAATSPDSNSSVGGFDILAGTSMAAPVVAGV 562

Query: 571 VALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPPKVADPFDYGGGVVDPNAAA 630
           VALLK+LHP WSPAA +SAI+TTA   +P G  IFA GS  KVADPFDYGGG+V+P  AA
Sbjct: 563 VALLKALHPNWSPAAFRSAIVTTAWRTDPFGEQIFAEGSSRKVADPFDYGGGIVNPEKAA 622

Query: 631 DPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQRPSMLDLNLPTITVPELTN 690
           DPGLIYD+G  DYI  YLC+ GY  S I+ L    T CS  + S+LD+NLP+IT+P+L +
Sbjct: 623 DPGLIYDMGPRDYI-LYLCSAGYNDSSITQLVGNVTVCSTPKTSVLDVNLPSITIPDLKD 682

Query: 691 STTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNSKVKKISFKVTISTTLSTNY 698
             T+TRTVTNVG + S+Y+ VVEPP   +V V P+ L FNSK K +SF V +STT   N 
Sbjct: 683 EVTLTRTVTNVGTVDSVYKVVVEPPLGIQVVVAPETLVFNSKTKNVSFTVRVSTTHKINT 742

[Close]

BLAST of Spg030021 vs. ExPASy TrEMBL
Match: A0A6J1JBL4 (subtilisin-like protease SBT3.9 OS=Cucurbita maxima OX=3661 GN=LOC111482985 PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 8.9e-253
Identity = 479/764 (62.70%), Postives = 555/764 (72.64%), Query Frame = 0


Query: 1   MVNSEAP-LIVSVMRFYVLFSMFAYKSMAEEADQNRKVHIVYLGERQYDDINLTTESHHN 60
           M N+ AP  I++V+  Y +FS      MAE  DQN KVHIVYLGER YDD+ LTT+SHH 
Sbjct: 1   MENNRAPSFILAVVTIYAVFS-----DMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHE 60

Query: 61  LLAKVLG-----------SYRHGFSGFAAKLTKSQAQKL--------------------- 120
           LL  VLG           SYRHGFSGFAAKLT SQAQKL                     
Sbjct: 61  LLESVLGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTR 120

Query: 121 ----------------------------VGVIDTGFWPESASFADKGMGPVPSRWKGKCK 180
                                       +GVIDTGFWPES SF DKGMGP+PSRWKG C+
Sbjct: 121 SWDFLGLSSSPSESSNLFHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQ 180

Query: 181 SGELFNSTNCNKKVIGARWFVKGFIADYGREALANECLSPRDTHGHGTHTASTAAGSFVT 240
           SGE FNS++CNKKVIGARWF    IAD+G EA+  + LS RD  GHGTHTASTA G+FV 
Sbjct: 181 SGEDFNSSHCNKKVIGARWFASALIADHGEEAVFKDYLSARDNEGHGTHTASTAGGAFVR 240

Query: 241 NVSYHGLGAGTLRGGAPLARLAIYKVGWTGGG-GSMADILKGIDVAIHDGVDVLSISMGL 300
           NVSY G G GTLRGGAPLARLAIYKV W+    GS ADILKGID AIHDGVDVLS+S+G 
Sbjct: 241 NVSYFGNGRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIGK 300

Query: 301 PLPLVPELDQANGIGIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMDRA 360
            +PL P+++  N + +GSFHAIA+GI VVC+GGNEG  +QTV N APW+ TVAAST+DRA
Sbjct: 301 SIPLFPDVNDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRA 360

Query: 361 FLSSITLLEDNTTYLGQSFFYSKKDLVAELTVPPDRRCDRISGNE-NFSGKVV-LCFSDK 420
           FL SIT L DN TYLGQ F   +KD+V  L V  D RC  I G +   SG VV LCF+D 
Sbjct: 361 FLVSITTLGDNATYLGQMFL--RKDIVGML-VAMDGRCAGILGGDIPISGNVVLLCFTDL 420

Query: 421 VNLS-AATAV---KKANGSGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDDSN 480
              + A+ AV   K+A   G+I AG+  D L       PC+ VDT VGTKLF   L+D +
Sbjct: 421 AKTAPASNAVMPGKQAKVVGVIYAGQQTDNLVPC--DIPCIHVDTHVGTKLFTYLLNDDD 480

Query: 481 PMVRLRHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSNDE 540
            ++RLR  +TIIGKPIS+RIA+FSSRGPNS SP ILKPDIA+PG+NI+AAVPP+    D+
Sbjct: 481 ALIRLRATRTIIGKPISSRIAYFSSRGPNSFSPGILKPDIAAPGANIIAAVPPNHKGGDK 540

Query: 541 GFGMMSGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPPK 600
           GF  MSGTSMA PH+SGIV L+KSLHPTWSPAAIKSA+ITTARV++PSG+PIFA GSPPK
Sbjct: 541 GFATMSGTSMATPHISGIVTLIKSLHPTWSPAAIKSALITTARVEDPSGMPIFAEGSPPK 600

Query: 601 VADPFDYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQR 660
           VADPFDYGGGVVD NAA DPGLIYDLG +DYIYYYLC+MGY  +DISHL+++KT C  +R
Sbjct: 601 VADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKR 660

Query: 661 PSMLDLNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNSK 697
            S+LDLNLPTITVP L NSTTVTRTVTNVGNLT++Y+AV++ PP +KV V+P+VL FNS 
Sbjct: 661 ASILDLNLPTITVPALINSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSN 720

[Close]

BLAST of Spg030021 vs. ExPASy TrEMBL
Match: A0A1S3BU23 (subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493167 PE=3 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 3.8e-251
Identity = 475/764 (62.17%), Postives = 545/764 (71.34%), Query Frame = 0


Query: 2   VNSE-APLIVSVMRFYVLFSMFAYKSMAEEADQNRKVHIVYLGERQYDDINLTTESHHNL 61
           +NS+ A LIV  + FY L        +AE  DQN KVHIVYLGE+ + D   TT+SHH L
Sbjct: 3   INSQLAHLIVGFIIFYGLCEPI----LAESNDQNPKVHIVYLGEKPHHDTKFTTDSHHQL 62

Query: 62  LAKVLG-----------SYRHGFSGFAAKLTKSQAQKL---------------------- 121
           LA +LG           SY+HGFSGFAAKLTKS+AQKL                      
Sbjct: 63  LAAILGSKEKSLEAMVYSYKHGFSGFAAKLTKSEAQKLSEMSRVVRVVPSSLYKVHTTRS 122

Query: 122 --------------------------VGVIDTGFWPESASFADKGMGPVPSRWKGKCKSG 181
                                     +GVID+G WPES SF DKG+GP+PSRWKG C+SG
Sbjct: 123 WDFLGLSSSPSESSNLLHRAKGDDVIIGVIDSGIWPESESFKDKGLGPIPSRWKGTCESG 182

Query: 182 ELFNSTNCNKKVIGARWFVKGFIADYGREALANECLSPRDTHGHGTHTASTAAGSFVTNV 241
           E FNSTNCNKK+IGARWFVK F+ADYGREALA E LSPRD HGHGTHTASTAAGSFV N+
Sbjct: 183 EQFNSTNCNKKIIGARWFVKAFVADYGREALAKEYLSPRDLHGHGTHTASTAAGSFVANI 242

Query: 242 SYHGLGAGTLRGGAPLARLAIYKVGWTGGG-GSMADILKGIDVAIHDGVDVLSISMGLPL 301
           +YH   AGT RGGAPLARLAIYK  WT  G GS ADILK ID AIHDGVDVLSIS+G   
Sbjct: 243 NYHNNAAGTARGGAPLARLAIYKALWTNRGVGSSADILKAIDEAIHDGVDVLSISIGGSP 302

Query: 302 PLVPELDQANGIGIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMDRAFL 361
           P  PE  + + I  GSFHAI +GI VVC+ GN GP  Q V N APWI TVA +T+DRAFL
Sbjct: 303 PFYPEFTELSDIAFGSFHAITKGISVVCAAGNSGPSPQMVDNVAPWIFTVATNTIDRAFL 362

Query: 362 SSITLLEDNTTYLGQSFFYSKKDLVAELTVPPDRRCDRISGNENF-SGKVVLCFSDKVNL 421
           SSIT L DNTT++GQS   SKKDLVAEL      RCD++SGNE F +GKVVLCF    + 
Sbjct: 363 SSITTLRDNTTFMGQSLLESKKDLVAELV--SWERCDQLSGNEAFINGKVVLCFPKLADY 422

Query: 422 S----AATAVKKANGSGIIVAGENID--ILYEFGESFPCVVVDTDVGTKLFFQALDDS-N 481
           S    AA  V +ANG+GIIVAG+ +D  +L       PC++VDT VG+KLFF  L +S +
Sbjct: 423 STISKAAEVVVRANGTGIIVAGQQVDNNLLACISSPIPCILVDTIVGSKLFFYFLQNSDH 482

Query: 482 PMVRLRHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSNDE 541
           P+V LR A+TIIGKPI+  IAHFSSRGPNS SP ILKPDI++PGSNIL+AV P +  N++
Sbjct: 483 PVVMLRAARTIIGKPIAPTIAHFSSRGPNSVSPPILKPDISAPGSNILSAVSPHYFFNEK 542

Query: 542 GFGMMSGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPPK 601
           GF +MSGTSMA PHVS IVALLKS+HPTWSPAAIKSA++TTAR +   GLPIFA G+PPK
Sbjct: 543 GFSLMSGTSMATPHVSAIVALLKSVHPTWSPAAIKSALMTTARTEVSPGLPIFADGTPPK 602

Query: 602 VADPFDYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQR 661
           VADPFDYG GVVD NAA DPGLIYD+G  DYI YYLC MGY+  DISHLT +KTEC LQR
Sbjct: 603 VADPFDYGAGVVDANAAVDPGLIYDMGRKDYIDYYLCGMGYEDKDISHLTHRKTECPLQR 662

Query: 662 PSMLDLNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNSK 697
            S+LDLNLP IT+P L NST VTRTVTNVGNL+ +Y+A +E P   KVSV PQVL FNS+
Sbjct: 663 LSLLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAKIEAPFGCKVSVNPQVLVFNSQ 722

[Close]

BLAST of Spg030021 vs. ExPASy TrEMBL
Match: A0A6J1EZL0 (subtilisin-like protease SBT3.7 OS=Cucurbita moschata OX=3662 GN=LOC111437655 PE=3 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 1.1e-250
Identity = 476/766 (62.14%), Postives = 558/766 (72.85%), Query Frame = 0


Query: 1   MVNSEAP---LIVSVMRFYVLFSMFAYKSMAEEADQNRKVHIVYLGERQYDDINLTTESH 60
           M N++AP   L+V+V+  YV+FS     +M E  DQN KVHIVYLGER YDD+ LTT+SH
Sbjct: 1   MENNQAPSFMLVVAVVTIYVVFS-----AMGEADDQNSKVHIVYLGERPYDDVTLTTDSH 60

Query: 61  HNLLAKVLG-----------SYRHGFSGFAAKLTKSQAQKL------------------- 120
           H LL  VLG           SYRHGFSGFAAKLT SQAQKL                   
Sbjct: 61  HELLESVLGSKEKSLESIVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSPLYKMRT 120

Query: 121 ------------------------------VGVIDTGFWPESASFADKGMGPVPSRWKGK 180
                                         +GVIDTGFWPES SF DKGMGP+PSRWKG 
Sbjct: 121 TRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGI 180

Query: 181 CKSGELFNSTNCNKKVIGARWFVKGFIADYGREALANECLSPRDTHGHGTHTASTAAGSF 240
           C+SGE FNS++CNKKVIGARWF    +AD+G EA+  + LS RD  GHGTHTASTA G+F
Sbjct: 181 CQSGEDFNSSHCNKKVIGARWFASALVADHGEEAVFMDYLSARDNEGHGTHTASTAGGAF 240

Query: 241 VTNVSYHGLGAGTLRGGAPLARLAIYKVGWTGGG-GSMADILKGIDVAIHDGVDVLSISM 300
           V NVSY G G GTLRGGAPLARLAIYKV W+    GS ADILKGID AIHDGVDVLS+S+
Sbjct: 241 VRNVSYFGNGRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSI 300

Query: 301 GLPLPLVPELDQANGIGIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMD 360
           G  +PL  ++++ N + +GSFHAIA+GI VVC+GGNEG  +QTV N APW+ TVAA+T+D
Sbjct: 301 GKSIPLFSDVNELNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTID 360

Query: 361 RAFLSSITLLEDNTTYLGQSFFYSKKDLVAELTVPPDRRCDRISG-NENFSGKVV-LCFS 420
           RAFL+SIT L DN TYLGQ+F   KKD+V  L +  DRRC  + G N   SG VV LCF+
Sbjct: 361 RAFLASITTLGDNATYLGQTFL--KKDIVGML-LAMDRRCAGLLGSNIPISGNVVLLCFT 420

Query: 421 DKVNLSAATAV----KKANGSGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDD 480
           D    + A+ V    K A   GII AG++ DIL       PC+ VDT VGT+LF   L+D
Sbjct: 421 DLAKKAGASNVVMPGKPAKVVGIIYAGQHNDILGPC--DTPCIHVDTHVGTQLFTYYLND 480

Query: 481 SNPMVRLRHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSN 540
               +R+R  +TI GKPIS+RIA+FSSRGPNS SP ILKPDIA+PG+NI+AAVPP+    
Sbjct: 481 EKAFIRVRATRTITGKPISSRIAYFSSRGPNSISPGILKPDIAAPGANIIAAVPPNHEGG 540

Query: 541 DEGFGMMSGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSP 600
           D+GF +MSGTSMAAPH+SGIVAL+KSL PTWSPA IKSA+ITTARV++ SGLPIFA GSP
Sbjct: 541 DKGFAVMSGTSMAAPHISGIVALIKSLRPTWSPAVIKSALITTARVEDLSGLPIFAEGSP 600

Query: 601 PKVADPFDYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSL 660
           PKVADPFDYGGGVVD NAA DPGLIYDL  +DYIYYYLC+MGY  +DISHL+++KT C  
Sbjct: 601 PKVADPFDYGGGVVDANAAIDPGLIYDLEVTDYIYYYLCSMGYTEADISHLSQQKTVCPS 660

Query: 661 QRPSMLDLNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFN 697
           +R S+LDLNLPTITVP LTNSTTVTRTVTNVGNLT++Y+AV++ PP +KV V+P+VL FN
Sbjct: 661 KRTSILDLNLPTITVPTLTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFN 720

[Close]

BLAST of Spg030021 vs. ExPASy TrEMBL
Match: A0A6J1EYU3 (subtilisin-like protease SBT3.7 isoform X6 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 872.1 bits (2252), Expect = 1.6e-249
Identity = 472/762 (61.94%), Postives = 554/762 (72.70%), Query Frame = 0


Query: 1   MVNSEAP---LIVSVMRFYVLFSMFAYKSMAEEADQNRKVHIVYLGERQYDDINLTTESH 60
           M N++AP   L V+++  Y +FS     +MAE  DQN KVHIVYLGER YDD+ LTT SH
Sbjct: 1   MENNQAPSFMLAVAIVTIYAVFS-----AMAEVDDQNSKVHIVYLGERPYDDVKLTTHSH 60

Query: 61  HNLLAKVLG-----------SYRHGFSGFAAKLTKSQAQKL------------------- 120
           H LL  VL            SY+HGFSGFAAKLT SQAQKL                   
Sbjct: 61  HELLESVLRSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLALARVFPSSLYKMHTTRSW 120

Query: 121 --------------------------VGVIDTGFWPESASFADKGMGPVPSRWKGKCKSG 180
                                     +GVID+GFWPES SF DKGMGP+PSRWKG C+ G
Sbjct: 121 DFLGLSSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQLG 180

Query: 181 ELFNSTNCNKKVIGARWFVKGFIADYGREALANECLSPRDTHGHGTHTASTAAGSFVTNV 240
           E FNS +CNKKVIGARWF +  IAD+G EA+  + LS RD  GHGTHTASTA G+FV NV
Sbjct: 181 EDFNSFHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNV 240

Query: 241 SYHGLGAGTLRGGAPLARLAIYKVGWTGGG-GSMADILKGIDVAIHDGVDVLSISMGLPL 300
           SY G   GTLRGGAPLARLAIYKV W+    GS ADILKGID AIHDGVDVLS+S+G  +
Sbjct: 241 SYFGNRRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIGGSI 300

Query: 301 PLVPELDQANGIGIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMDRAFL 360
           PL P++ + N + IGSFHAIARG+ VVC+GGNEG  +QTV N APW+ TVAAST+DRAFL
Sbjct: 301 PLPPDVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAFL 360

Query: 361 SSITLLEDNTTYLGQSFFYSKKDLVAELTVPPDRRCDRISGNENFSG--KVVLCFSDKVN 420
           +SIT L DN TYLGQ+F   KKD+V +L V  DRRC    G++  +G   V+LCF D   
Sbjct: 361 TSITTLGDNATYLGQTFL--KKDVVGKLVV-MDRRCAGHLGSDIPTGGNVVLLCFIDLAM 420

Query: 421 LSAAT----AVKKANGSGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDDSNPM 480
            +AA+     VK+A   G+I AG++ DIL       PC+ VDT VGTKL    L D   +
Sbjct: 421 KAAASNPVMPVKQAKVVGVIYAGQHTDILGPC--DVPCIHVDTHVGTKLLTYILSDEKAL 480

Query: 481 VRLRHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSNDEGF 540
           +RL+  +TI+GKPIS+RIA+FSSRGPNS SP ILKPDIA+PGSNI+AAVPP+    D+GF
Sbjct: 481 IRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDKGF 540

Query: 541 GMMSGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPPKVA 600
             MSGTSMA PH+SGIVAL+KSL PTWSPAAIKSA+ITTARV++PSGLPIFA GSPPKVA
Sbjct: 541 AAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPKVA 600

Query: 601 DPFDYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQRPS 660
           DPFDYGGGVVD NAA DPGLIYDLG +DYIYYY+C+MGY  ++ISHL+++K  C  +R S
Sbjct: 601 DPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRAS 660

Query: 661 MLDLNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNSKVK 697
           +LDLNLPTITVP LTNSTTVTRTVTNVGNLT++Y+AV++ PP +KV V P+VL FNS VK
Sbjct: 661 VLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNVK 720

[Close]

BLAST of Spg030021 vs. ExPASy TrEMBL
Match: A0A6J1EZK4 (subtilisin-like protease SBT3.7 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 870.5 bits (2248), Expect = 4.6e-249
Identity = 472/766 (61.62%), Postives = 554/766 (72.32%), Query Frame = 0


Query: 1   MVNSEAP---LIVSVMRFYVLFSMFAYKSMAEEADQNRKVHIVYLGERQYDDINLTTESH 60
           M N++AP   L V+++  Y +FS     +MAE  DQN KVHIVYLGER YDD+ LTT SH
Sbjct: 1   MENNQAPSFMLAVAIVTIYAVFS-----AMAEVDDQNSKVHIVYLGERPYDDVKLTTHSH 60

Query: 61  HNLLAKVLG-----------SYRHGFSGFAAKLTKSQAQKL------------------- 120
           H LL  VL            SY+HGFSGFAAKLT SQAQKL                   
Sbjct: 61  HELLESVLRSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHT 120

Query: 121 ------------------------------VGVIDTGFWPESASFADKGMGPVPSRWKGK 180
                                         +GVID+GFWPES SF DKGMGP+PSRWKG 
Sbjct: 121 TRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGI 180

Query: 181 CKSGELFNSTNCNKKVIGARWFVKGFIADYGREALANECLSPRDTHGHGTHTASTAAGSF 240
           C+ GE FNS +CNKKVIGARWF +  IAD+G EA+  + LS RD  GHGTHTASTA G+F
Sbjct: 181 CQLGEDFNSFHCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAF 240

Query: 241 VTNVSYHGLGAGTLRGGAPLARLAIYKVGWTGGG-GSMADILKGIDVAIHDGVDVLSISM 300
           V NVSY G   GTLRGGAPLARLAIYKV W+    GS ADILKGID AIHDGVDVLS+S+
Sbjct: 241 VRNVSYFGNRRGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSI 300

Query: 301 GLPLPLVPELDQANGIGIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMD 360
           G  +PL P++ + N + IGSFHAIARG+ VVC+GGNEG  +QTV N APW+ TVAAST+D
Sbjct: 301 GGSIPLPPDVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTID 360

Query: 361 RAFLSSITLLEDNTTYLGQSFFYSKKDLVAELTVPPDRRCDRISGNENFSG--KVVLCFS 420
           RAFL+SIT L DN TYLGQ+F   KKD+V +L V  DRRC    G++  +G   V+LCF 
Sbjct: 361 RAFLTSITTLGDNATYLGQTFL--KKDVVGKLVV-MDRRCAGHLGSDIPTGGNVVLLCFI 420

Query: 421 DKVNLSAAT----AVKKANGSGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDD 480
           D    +AA+     VK+A   G+I AG++ DIL       PC+ VDT VGTKL    L D
Sbjct: 421 DLAMKAAASNPVMPVKQAKVVGVIYAGQHTDILGPC--DVPCIHVDTHVGTKLLTYILSD 480

Query: 481 SNPMVRLRHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSN 540
              ++RL+  +TI+GKPIS+RIA+FSSRGPNS SP ILKPDIA+PGSNI+AAVPP+    
Sbjct: 481 EKALIRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGG 540

Query: 541 DEGFGMMSGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSP 600
           D+GF  MSGTSMA PH+SGIVAL+KSL PTWSPAAIKSA+ITTARV++PSGLPIFA GSP
Sbjct: 541 DKGFAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSP 600

Query: 601 PKVADPFDYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSL 660
           PKVADPFDYGGGVVD NAA DPGLIYDLG +DYIYYY+C+MGY  ++ISHL+++K  C  
Sbjct: 601 PKVADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPS 660

Query: 661 QRPSMLDLNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFN 697
           +R S+LDLNLPTITVP LTNSTTVTRTVTNVGNLT++Y+AV++ PP +KV V P+VL FN
Sbjct: 661 KRASVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFN 720

[Close]

BLAST of Spg030021 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 674.9 bits (1740), Expect = 7.1e-194
Identity = 374/766 (48.83%), Postives = 484/766 (63.19%), Query Frame = 0


Query: 16  YVLFSMFAY----KSMAEEADQNRKVHIVYLGERQYDDINLTTESHHNLLAKVLG----- 75
           YV+ S+  +    +S   E+   RKVHIVYLGE+Q+DD    TESHH +L  +LG     
Sbjct: 9   YVVLSLVIFLNVQRSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDA 68

Query: 76  ------SYRHGFSGFAAKLTKSQAQK---------------------------------- 135
                 SYRHGFSGFAAKLT+SQA+K                                  
Sbjct: 69  NDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANP 128

Query: 136 -------------LVGVIDTGFWPESASFADKGMGPVPSRWKGKCKSGELFNSTNCNKKV 195
                        ++GVIDTG WPES  F D G GPVPS WKG C++GE FNS+NCNKK+
Sbjct: 129 KSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKL 188

Query: 196 IGARWFVKGFIADYGREALAN--ECLSPRDTHGHGTHTASTAAGSFVTNVSYHGLGAGTL 255
           IGA++F+ GF+A+       N  + +SPRD  GHGTH ++ A GSFV N+SY GL  GT+
Sbjct: 189 IGAKYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTV 248

Query: 256 RGGAPLARLAIYKVGWTGGGG-----SMADILKGIDVAIHDGVDVLSISMGLPLPLVPEL 315
           RGGAP A +A+YK  W          S ADILK +D A+HDGVDVLSIS+G  +PL  E 
Sbjct: 249 RGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGET 308

Query: 316 DQANGIGIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMDRAFLSSITLL 375
           D  +GI  G+FHA+ +GI VVCSGGN GP   TV NTAPWI+TVAA+T+DR+F + +T L
Sbjct: 309 DIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLT-L 368

Query: 376 EDNTTYLGQSFFYSKKDLVAELTVP--PDRRCDRISG---------NENFSGKVVLCFSD 435
            +N   LGQ+ +         L  P  P    +  SG         N    GKVVLCF+ 
Sbjct: 369 GNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVVLCFTT 428

Query: 436 K----VNLSAATAVKKANGSGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDDS 495
                  LSAA  VK+A G G+I+A      +    + FPCV VD ++GT +        
Sbjct: 429 SPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSG 488

Query: 496 NPMVRLRHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSND 555
           +P+V+++ +KT++G+P+ T++A FSSRGPNS +PAILKPDIA+PG +ILAA   + + +D
Sbjct: 489 SPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAAT-TNTTFSD 548

Query: 556 EGFGMMSGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPP 615
           +GF M+SGTSMAAP +SG+ ALLK+LH  WSPAAI+SAI+TTA   +P G  IFA GSPP
Sbjct: 549 QGFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPP 608

Query: 616 KVADPFDYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQ 675
           K+ADPFDYGGG+V+P  +A+PGL+YD+G  DY+  Y+C++GY  + IS L  K T CS  
Sbjct: 609 KLADPFDYGGGLVNPEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQLIGKTTVCSNP 668

Query: 676 RPSMLDLNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNS 698
           +PS+LD NLP+IT+P L +  T+TRTVTNVG L S+YR  VEPP   +V+V P+ L FNS
Sbjct: 669 KPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNS 728

[Close]

BLAST of Spg030021 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 674.5 bits (1739), Expect = 9.3e-194
Identity = 371/753 (49.27%), Postives = 478/753 (63.48%), Query Frame = 0


Query: 25  KSMAEEADQNRKVHIVYLGERQYDDINLTTESHHNLLAKVLG-----------SYRHGFS 84
           +S   E+   RKVHIVYLGE+Q+DD    TESHH +L  +LG           SYRHGFS
Sbjct: 38  RSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFS 97

Query: 85  GFAAKLTKSQAQK----------------------------------------------- 144
           GFAAKLT+SQA+K                                               
Sbjct: 98  GFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKSLLHETNMGEQI 157

Query: 145 LVGVIDTGFWPESASFADKGMGPVPSRWKGKCKSGELFNSTNCNKKVIGARWFVKGFIAD 204
           ++GVIDTG WPES  F D G GPVPS WKG C++GE FNS+NCNKK+IGA++F+ GF+A+
Sbjct: 158 IIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKLIGAKYFINGFLAE 217

Query: 205 YGREALAN--ECLSPRDTHGHGTHTASTAAGSFVTNVSYHGLGAGTLRGGAPLARLAIYK 264
                  N  + +SPRD  GHGTH ++ A GSFV N+SY GL  GT+RGGAP A +A+YK
Sbjct: 218 NESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAMYK 277

Query: 265 VGWTGGGG-----SMADILKGIDVAIHDGVDVLSISMGLPLPLVPELDQANGIGIGSFHA 324
             W          S ADILK +D A+HDGVDVLSIS+G  +PL  E D  +GI  G+FHA
Sbjct: 278 ACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGETDIRDGITTGAFHA 337

Query: 325 IARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMDRAFLSSITLLEDNTTYLGQSFFY 384
           + +GI VVCSGGN GP   TV NTAPWI+TVAA+T+DR+F + +T L +N   LGQ+ + 
Sbjct: 338 VLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLT-LGNNKVILGQAMYT 397

Query: 385 SKKDLVAELTVP--PDRRCDRISG---------NENFSGKVVLCFSDK----VNLSAATA 444
                   L  P  P    +  SG         N    GKVVLCF+        LSAA  
Sbjct: 398 GPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVVLCFTTSPYGGAVLSAARY 457

Query: 445 VKKANGSGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDDSNPMVRLRHAKTII 504
           VK+A G G+I+A      +    + FPCV VD ++GT +        +P+V+++ +KT++
Sbjct: 458 VKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKTLV 517

Query: 505 GKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSNDEGFGMMSGTSMAA 564
           G+P+ T++A FSSRGPNS +PAILKPDIA+PG +ILAA   + + +D+GF M+SGTSMAA
Sbjct: 518 GQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAAT-TNTTFSDQGFIMLSGTSMAA 577

Query: 565 PHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPPKVADPFDYGGGVV 624
           P +SG+ ALLK+LH  WSPAAI+SAI+TTA   +P G  IFA GSPPK+ADPFDYGGG+V
Sbjct: 578 PAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPPKLADPFDYGGGLV 637

Query: 625 DPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQRPSMLDLNLPTIT 684
           +P  +A+PGL+YD+G  DY+  Y+C++GY  + IS L  K T CS  +PS+LD NLP+IT
Sbjct: 638 NPEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQLIGKTTVCSNPKPSVLDFNLPSIT 697

Query: 685 VPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNSKVKKISFKVTIST 698
           +P L +  T+TRTVTNVG L S+YR  VEPP   +V+V P+ L FNS  KK+ FKV +ST
Sbjct: 698 IPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNSTTKKVYFKVKVST 757

[Close]

BLAST of Spg030021 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 669.8 bits (1727), Expect = 2.3e-192
Identity = 378/760 (49.74%), Postives = 482/760 (63.42%), Query Frame = 0


Query: 17  VLFSMFAYKSMAEEADQNRKVHIVYLGERQYDDINLTTESHHNLLAKVLG---------- 76
           V+ S+    ++A  A    KVHIVYLGE+Q+DD    TESHH +L+ +LG          
Sbjct: 10  VVLSLIIVLNVA-RASAKSKVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMV 69

Query: 77  -SYRHGFSGFAAKLTKSQAQK--------------------------------------- 136
            SYRHGFSGFAAKLTKSQA+K                                       
Sbjct: 70  YSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYELATTRIWDYLGPSADNSKNLVS 129

Query: 137 --------LVGVIDTGFWPESASFADKGMGPVPSRWKGKCKSGELFNSTNCNKKVIGARW 196
                   ++GVIDTG WPES SF D G+GPVPS WKG C+ GE F STNCN+K+IGA++
Sbjct: 130 DTNMGDQTIIGVIDTGVWPESESFNDYGVGPVPSHWKGGCEPGENFISTNCNRKLIGAKY 189

Query: 197 FVKGFIADYGREALAN-ECLSPRDTHGHGTHTASTAAGSFVTNVSYHGLGAGTLRGGAPL 256
           F+ GF+A+    A  + + +S RD  GHGTH AS A GSFV NVSY GLG GTLRGGAP 
Sbjct: 190 FINGFLAENQFNATESPDYISARDFDGHGTHVASIAGGSFVPNVSYKGLGRGTLRGGAPR 249

Query: 257 ARLAIYKVGW-----TGGGGSMADILKGIDVAIHDGVDVLSISMGLPLPLVPELDQANGI 316
           AR+A+YK  W      G   S +DI+K ID AIHDGVDVLSIS+G  +PL  E D  +GI
Sbjct: 250 ARIAMYKACWYINELDGVTCSFSDIMKAIDEAIHDGVDVLSISLGGRVPLNSETDLRDGI 309

Query: 317 GIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMDRAFLSSITLLEDNTTY 376
             G+FHA+A+GI VVC+GGN GP  QTV NTAPWILTVAA+T+DR+F + I +L +N   
Sbjct: 310 ATGAFHAVAKGIVVVCAGGNAGPSSQTVVNTAPWILTVAATTLDRSFATPI-ILGNNQVI 369

Query: 377 LGQSFFYSKKDLVAELTVP--PDRRCDRISG---------NENFSGKVVLCFSD----KV 436
           LGQ+ +   +     L  P  P    D  SG         N   +GKVVLCF+      V
Sbjct: 370 LGQAMYIGPELGFTSLVYPEDPGNSIDTFSGVCESLNLNSNRTMAGKVVLCFTTARDFTV 429

Query: 437 NLSAATAVKKANGSGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDDSNPMVRL 496
             +AA+ VK A G G+I+A      L    + FPCV +D ++GT + F      +P+V++
Sbjct: 430 VSTAASIVKAAGGLGLIIARNPGYNLAPCSDDFPCVAIDNELGTDILFYIRYTGSPVVKI 489

Query: 497 RHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSNDEGFGMM 556
           + ++T++G+P+ T++A FSSRGPNS SPAILKPDIA+PG +ILAA  P+ + N  GF M 
Sbjct: 490 QPSRTLVGEPVGTKVATFSSRGPNSISPAILKPDIAAPGVSILAATSPNDTLNAGGFVMR 549

Query: 557 SGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPPKVADPF 616
           SGTSMAAP +SG++ALLKSLHP WSPAA +SAI+TTA   +P G  I A  S  KV DPF
Sbjct: 550 SGTSMAAPVISGVIALLKSLHPDWSPAAFRSAIVTTAWRTDPFGEQIAAESSSLKVPDPF 609

Query: 617 DYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQRPSMLD 676
           DYGGG+V+P  AA+PGLI D+ + DY+  YLC+ GY  S IS L  K T CS  +PS+LD
Sbjct: 610 DYGGGLVNPEKAAEPGLILDMDSQDYV-LYLCSAGYNDSSISRLVGKVTVCSNPKPSVLD 669

Query: 677 LNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNSKVKKIS 698
           +NLP+IT+P L +  T+TRTVTNVG + S+Y+ +VEPP   +V V P+ L FNSK K +S
Sbjct: 670 INLPSITIPNLKDEVTLTRTVTNVGPVDSVYKVLVEPPLGIQVVVTPETLVFNSKTKSVS 729

[Close]

BLAST of Spg030021 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 666.8 bits (1719), Expect = 1.9e-191
Identity = 371/764 (48.56%), Postives = 476/764 (62.30%), Query Frame = 0


Query: 16  YVLFSMFAYKSMAEEADQNRKVHIVYLGERQYDDINLTTESHHNLLAKVLG--------- 75
           +V   +    +    A    KVHIVYLGE+Q+DD    TESHH +L  +LG         
Sbjct: 9   FVAIILNGLSTFVAHAGAESKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDAHSSM 68

Query: 76  --SYRHGFSGFAAKLTKSQAQKL------------------------------------- 135
             SYRHGFSGFAAKLTKSQA+KL                                     
Sbjct: 69  VHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDYLGLSVANPKNLL 128

Query: 136 ----------VGVIDTGFWPESASFADKGMGPVPSRWKGKCKSGELFNSTNCNKKVIGAR 195
                     +G++D+G WPES  F D G+GPVPS WKG C SGE F S+ CNKK+IGA+
Sbjct: 129 NDTNMGEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENFTSSQCNKKLIGAK 188

Query: 196 WFVKGFIADY----GREALANECLSPRDTHGHGTHTASTAAGSFVTNVSYHGLGAGTLRG 255
           +F+ GF+A +      E+L  + +SPRD  GHGTH A+ A GS+V ++SY GL  GT+RG
Sbjct: 189 YFINGFLATHESFNSTESL--DFISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRG 248

Query: 256 GAPLARLAIYKVGW-----TGGGGSMADILKGIDVAIHDGVDVLSISMGLPLPLVPELDQ 315
           GAP AR+A+YK  W          S ADILK +D A+HDGVDVLS+S+G   P  PE D 
Sbjct: 249 GAPRARIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSIGYRFPYFPETDV 308

Query: 316 ANGIGIGSFHAIARGIPVVCSGGNEGPYEQTVGNTAPWILTVAASTMDRAFLSSITLLED 375
              I  G+FHA+ +GI VVCSGGN GP  QTVGNTAPWILTVAA+T+DR+F + IT L +
Sbjct: 309 RAVIATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPIT-LGN 368

Query: 376 NTTYLGQSFFYSKKDLVAELTVP--PDRRCDRISG---------NENFSGKVVLCFSDKV 435
           N   LGQ+ +   +     L  P  P    +  SG         N   +GKVVLCF+   
Sbjct: 369 NKLILGQAMYTGPELGFTSLVYPENPGNSNESFSGDCELLFFNSNHTMAGKVVLCFTTST 428

Query: 436 NL----SAATAVKKANGSGIIVAGENIDILYEFGESFPCVVVDTDVGTKLFFQALDDSNP 495
                 SA + VK+A G G+IVA    D L    + FPCV VD ++GT +         P
Sbjct: 429 RYITVSSAVSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGTDILLYIRSTGLP 488

Query: 496 MVRLRHAKTIIGKPISTRIAHFSSRGPNSASPAILKPDIASPGSNILAAVPPDFSSNDEG 555
           +V+++ +KT++G+P+ T++A FSSRGPNS  PAILKPDIA+PG +ILAA   + + ND G
Sbjct: 489 VVKIQPSKTLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILAATTTNKTFNDRG 548

Query: 556 FGMMSGTSMAAPHVSGIVALLKSLHPTWSPAAIKSAIITTARVKNPSGLPIFASGSPPKV 615
           F  +SGTSMAAP +SG+VALLK+LH  WSPAAI+SAI+TTA   +P G  IFA GSP K+
Sbjct: 549 FIFLSGTSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKL 608

Query: 616 ADPFDYGGGVVDPNAAADPGLIYDLGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQRP 675
           ADPFDYGGG+V+P  AA PGL+YDLG  DY+  Y+C++GY  + IS L  K T CS  +P
Sbjct: 609 ADPFDYGGGLVNPEKAAKPGLVYDLGLEDYV-LYMCSVGYNETSISQLVGKGTVCSNPKP 668

Query: 676 SMLDLNLPTITVPELTNSTTVTRTVTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNSKV 698
           S+LD NLP+IT+P L +  T+TRT+TNVG L S+Y+ V+EPP   +V+V P+ L FNS  
Sbjct: 669 SVLDFNLPSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVTPETLLFNSTT 728

[Close]

BLAST of Spg030021 vs. TAIR 10
Match: AT4G10510.1 (Subtilase family protein )

HSP 1 Score: 665.6 bits (1716), Expect = 4.3e-191
Identity = 363/740 (49.05%), Postives = 480/740 (64.86%), Query Frame = 0


Query: 37  VHIVYLGERQYDDINLTTESHHNLLAKVLG-----------SYRHGFSGFAAKLTKSQAQ 96
           VHIVYLGE+Q+DD    TESHH +L  +LG           S+RHGFSGFAAKLT+SQA+
Sbjct: 22  VHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEEAHGSMVHSFRHGFSGFAAKLTESQAK 81

Query: 97  K-----------------------------------------------LVGVIDTGFWPE 156
           K                                               ++G+ID+G WPE
Sbjct: 82  KIADLPEVVHVIPDRFYKPATTRTWDYLGLSPTNPKNLLNQTNMGEQMIIGIIDSGVWPE 141

Query: 157 SASFADKGMGPVPSRWKGKCKSGELFNSTNCNKKVIGARWFVKGFIADYG--REALANEC 216
           S  F D  +GPVPS WKG C+SGE FNS++CNKK+IGA++F+  F+A +     + + + 
Sbjct: 142 SEVFNDNEIGPVPSHWKGGCESGEDFNSSHCNKKLIGAKYFINAFLATHESFNSSESLDF 201

Query: 217 LSPRDTHGHGTHTASTAAGSFVTNVSYHGLGAGTLRGGAPLARLAIYKVGW----TGGGG 276
           +SPR  +GHGTH A+ A GS+V N SY GL  GT+RGGAP AR+A+YK  W         
Sbjct: 202 ISPRGYNGHGTHVATIAGGSYVPNTSYKGLAGGTVRGGAPRARIAVYKTCWYLDLDIAAC 261

Query: 277 SMADILKGIDVAIHDGVDVLSISMGLPLPLVPELDQANGIGIGSFHAIARGIPVVCSGGN 336
           S ADILK +D AIHDGVDVLS+S+G   PL PE D  +GI  G+FHA+ +GI VVC+ GN
Sbjct: 262 SSADILKAMDEAIHDGVDVLSLSLGFE-PLYPETDVRDGIATGAFHAVLKGITVVCAAGN 321

Query: 337 EGPYEQTVGNTAPWILTVAASTMDRAFLSSITLLEDNTTYLGQSFFYSKKDLVAELTVPP 396
            GP  QTVGNTAPWILTVAA+T+DR+F++ +T L +N   LGQ+ +   +     L  P 
Sbjct: 322 AGPAAQTVGNTAPWILTVAATTLDRSFVTPMT-LGNNKVILGQAIYTGTEVGFTSLVYPE 381

Query: 397 D---------RRCDR--ISGNENFSGKVVLCFSD---KVNLS-AATAVKKANGSGIIVAG 456
           +           C+R  I+ N   +GKVVLCF++    ++++ AA  VK+A G G+I+AG
Sbjct: 382 NPGNSNESFSGTCERLLINSNRTMAGKVVLCFTESPYSISVTRAAHYVKRAGGLGVIIAG 441

Query: 457 ENIDILYEFGESFPCVVVDTDVGTKLFFQALDDSNPMVRLRHAKTIIGKPISTRIAHFSS 516
           +  ++L    + FPCV VD ++GT + F    + +P+V+++ ++T+IG+P+ T++A FSS
Sbjct: 442 QPGNVLRPCLDDFPCVAVDYELGTYILFYIRSNGSPVVKIQPSRTLIGQPVGTKVASFSS 501

Query: 517 RGPNSASPAILKPDIASPGSNILAAVPPDFSSNDEGFGMMSGTSMAAPHVSGIVALLKSL 576
           RGPN  S AILKPDIA+PG +ILAA   + + ND GF  +SGTSMA P +SGIVALLK+L
Sbjct: 502 RGPNPISAAILKPDIAAPGVSILAATTTNTTFNDRGFIFLSGTSMATPTISGIVALLKAL 561

Query: 577 HPTWSPAAIKSAIITTARVKNPSGLPIFASGSPPKVADPFDYGGGVVDPNAAADPGLIYD 636
           HP WSPAAI+SAI+TTA   +P G  IFA GSP K ADPFDYGGG+V+P  A  PGL+YD
Sbjct: 562 HPDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKPADPFDYGGGLVNPEKATKPGLVYD 621

Query: 637 LGTSDYIYYYLCAMGYKVSDISHLTKKKTECSLQRPSMLDLNLPTITVPELTNSTTVTRT 696
           LG  DY+  Y+C++GY  + IS L  K T CS  +PS+LD NLP+IT+P L    T+ RT
Sbjct: 622 LGLEDYV-LYMCSVGYNETSISQLVGKGTVCSYPKPSVLDFNLPSITIPNLKEEVTLPRT 681

Query: 697 VTNVGNLTSIYRAVVEPPPSTKVSVEPQVLAFNSKVKKISFKVTISTTLSTNYGYSFGTL 698
           +TNVG L S+YR  VEPP  T+V+V P+ L FNS  K++SFKV++STT   N GY FG+L
Sbjct: 682 LTNVGPLESVYRVAVEPPLGTQVTVTPETLVFNSTTKRVSFKVSVSTTHKINTGYYFGSL 741

The following BLAST results are available for this feature:

BLAST of Spg030021 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023552781.1	1.1e-252	62.35	subtilisin-like protease SBT3.7 [Cucurbita pepo subsp. pepo]	[more]
XP_022984814.1	1.8e-252	62.70	subtilisin-like protease SBT3.9 [Cucurbita maxima]	[more]
XP_008452040.1	7.8e-251	62.17	PREDICTED: subtilisin-like protease SBT3.7 isoform X1 [Cucumis melo]	[more]
KAG6577195.1	1.7e-250	61.83	Subtilisin-like protease 3.8, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022931500.1	2.3e-250	62.14	subtilisin-like protease SBT3.7 [Cucurbita moschata]	[more]

BLAST of Spg030021 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L7I2	1.0e-192	48.83	Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 S...	[more]
F4HPF1	3.2e-191	49.74	Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 S...	[more]
Q9SZY2	9.4e-191	48.93	Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 S...	[more]
Q9SZY3	2.7e-190	48.56	Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 S...	[more]
Q9MAP7	3.9e-189	48.86	Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 S...	[more]

BLAST of Spg030021 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JBL4	8.9e-253	62.70	subtilisin-like protease SBT3.9 OS=Cucurbita maxima OX=3661 GN=LOC111482985 PE=3...	[more]
A0A1S3BU23	3.8e-251	62.17	subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034931...	[more]
A0A6J1EZL0	1.1e-250	62.14	subtilisin-like protease SBT3.7 OS=Cucurbita moschata OX=3662 GN=LOC111437655 PE...	[more]
A0A6J1EYU3	1.6e-249	61.94	subtilisin-like protease SBT3.7 isoform X6 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1EZK4	4.6e-249	61.62	subtilisin-like protease SBT3.7 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]

BLAST of Spg030021 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10550.1	7.1e-194	48.83	Subtilase family protein	[more]
AT4G10550.3	9.3e-194	49.27	Subtilase family protein	[more]
AT1G32950.1	2.3e-192	49.74	Subtilase family protein	[more]
AT4G10540.1	1.9e-191	48.56	Subtilase family protein	[more]
AT4G10510.1	4.3e-191	49.05	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015500	Peptidase S8, subtilisin-related	PRINTS	PR00723	SUBTILISIN	coord: 83..102 score: 24.53 coord: 478..494 score: 61.53 coord: 161..174 score: 49.36
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 51..87 e-value: 9.4E-5 score: 23.0
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 90..563 e-value: 3.4E-153 score: 512.8
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 87..554
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 87..516 e-value: 2.6E-45 score: 154.9
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 597..692 e-value: 9.0E-28 score: 96.4
None	No IPR available	GENE3D	3.50.30.30		coord: 296..424 e-value: 3.4E-153 score: 512.8
None	No IPR available	GENE3D	2.60.40.2310		coord: 568..694 e-value: 5.4E-37 score: 128.4
None	No IPR available	PANTHER	PTHR43806	PEPTIDASE S8	coord: 26..87 coord: 86..694
None	No IPR available	PANTHER	PTHR43806:SF50	SUBTILISIN-LIKE PROTEASE SBT3.1-RELATED	coord: 26..87 coord: 86..694
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 58..550 score: 26.873055
None	No IPR available	CDD	cd02120	PA_subtilisin_like	coord: 306..417 e-value: 6.87995E-11 score: 58.1939
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 22..89 e-value: 8.4E-11 score: 44.2
IPR023828	Peptidase S8, subtilisin, Ser-active site	PROSITE	PS00138	SUBTILASE_SER	coord: 479..489
IPR034197	Cucumisin-like catalytic domain	CDD	cd04852	Peptidases_S8_3	coord: 88..514 e-value: 2.4921E-126 score: 375.398

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg030021.1	Spg030021.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity