Spg030004 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg030004 vs. NCBI nr
Match: XP_023530877.1 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 4.3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1063.9 bits (2750), Expect = 5.1e-307
Identity = 553/614 (90.07%), Postives = 577/614 (93.97%), Query Frame = 0

Query: 1   MEMEMDIETTRRDHFKGEIITHSLPSDQDLTVDWRGRPSSPVTHGGMRAAAFVLGLQAFE 60
           MEM+MD++  RR        TH +PS QDLTVDWRGRPS+PV HGGMRAAAFVLGLQ+FE
Sbjct: 5   MEMDMDMDIERRRD------THLMPS-QDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFE 64

Query: 61  IMAIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLI 120
           IMAIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLAL+GGFLSDSYLGSFWTMLI
Sbjct: 65  IMAIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLI 124

Query: 121 FGFVELSGFILLSVQAHLPQLKPPQCNMLSEGEECVEAKGLKALIFFVALYLVALGSGCV 180
           FGFVELSGFILLSVQAHLPQLKPPQCNMLSEG+ CVEAKG+KALIFFVALYLVALGSGCV
Sbjct: 125 FGFVELSGFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCV 184

Query: 181 KPNMIAHGADQFTQQNPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGV 240
           KPNMIAHGADQFT Q+PSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGV
Sbjct: 185 KPNMIAHGADQFTPQHPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGV 244

Query: 241 SAALMAMGLISLVAGIFYYRNKPPQGAIFIPILQ--------RKQVCPSNPQMLHGSQTS 300
           SAA+MAMGLISLV GIFYYRNKPPQGAIFIPILQ        RKQVCPSNPQMLHGSQTS
Sbjct: 245 SAAVMAMGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTS 304

Query: 301 NDKVVALSSDSANLVPTQKFRFLDKACIKSQDGATKESPWRLCTPTQVEQVKILISVVPI 360
           N K VALSS SANL+PTQKFRFLDKAC+KSQDG T+ESPWRLCTPTQVEQVKILISVVPI
Sbjct: 305 NHK-VALSSHSANLIPTQKFRFLDKACVKSQDGTTRESPWRLCTPTQVEQVKILISVVPI 364

Query: 361 FACTIIFNTILAQLQTFSVQQGSLMDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFV 420
           FACTIIFNTILAQLQTFSVQQGSLM+TQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFV
Sbjct: 365 FACTIIFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFV 424

Query: 421 PFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKRRRDSAVNLNQTISIFWITPQF 480
           PFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEK+RRDSAV+ NQTISIFWITPQF
Sbjct: 425 PFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVDSNQTISIFWITPQF 484

Query: 481 LIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSS 540
           LIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKITSSS+
Sbjct: 485 LIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSST 544

Query: 541 NGGWLHDNNLNKDRLDLFYWLLAALSFLNFFNYLFWSTWFEEHPSLSQKPQHDNNLHHGE 600
           NGGWLHDNNLNKDRLDLFYWLLAALSFLNF NYLFWS+WF++ PSLSQK Q D NLHHGE
Sbjct: 545 NGGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQD-NLHHGE 604

Query: 601 SHHIYNFDSSKNNV 607
           + HIYN + SKNNV
Sbjct: 605 NXHIYNNNFSKNNV 609

BLAST of Spg030004 vs. NCBI nr
Match: XP_038903187.1 (protein NRT1/ PTR FAMILY 4.3-like [Benincasa hispida])

HSP 1 Score: 1056.6 bits (2731), Expect = 8.2e-305
Identity = 545/613 (88.91%), Postives = 576/613 (93.96%), Query Frame = 0

Query: 7   IETTRRDHFKGEIITHSLPSDQDLTVDWRGRPSSPVTHGGMRAAAFVLGLQAFEIMAIAA 66
           +E  R+  FKGE  +H++PS +D++VDWRGRPS+P +HGGMRAAAFVLGLQAFEIMAIAA
Sbjct: 1   MEMERKRDFKGE--SHTIPS-EDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAA 60

Query: 67  VGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVEL 126
           VGNNLITY+INEMHFSLSKSAN+VTNFVGTVFLLAL+GGFLSDSYLGSFWT+LIFGFVEL
Sbjct: 61  VGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVEL 120

Query: 127 SGFILLSVQAHLPQLKPPQCNMLSEGEECVEAKGLKALIFFVALYLVALGSGCVKPNMIA 186
           SGFILLSVQAH+PQLKPPQCNML + +ECVEAKG+KALIFFVALYLVALGSGCVKPNMIA
Sbjct: 121 SGFILLSVQAHVPQLKPPQCNMLLKSDECVEAKGVKALIFFVALYLVALGSGCVKPNMIA 180

Query: 187 HGADQFTQQNPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAALMA 246
           HGADQF+ QN SQSK LS YFNAAYFAFSIGELIALTL+VWIQTHSGMDVGFGVSAA+MA
Sbjct: 181 HGADQFSPQNSSQSKSLSKYFNAAYFAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMA 240

Query: 247 MGLISLVAGIFYYRNKPPQGAIFIPILQ--------RKQVCPSNPQMLHGSQTSNDKVVA 306
           +GLI LVAGIFYYRNKPPQG IFIPILQ        RKQVCPSN  MLHGSQTSNDK VA
Sbjct: 241 LGLIILVAGIFYYRNKPPQGPIFIPILQVFVAALLKRKQVCPSNSHMLHGSQTSNDK-VA 300

Query: 307 LSSDSANLVPTQKFRFLDKACIKSQDGATKESPWRLCTPTQVEQVKILISVVPIFACTII 366
           LSSDSANL+PTQKFRFLDKAC KSQDGATKESPWRLCTP QVEQVKILISVVPIFACTII
Sbjct: 301 LSSDSANLIPTQKFRFLDKACFKSQDGATKESPWRLCTPNQVEQVKILISVVPIFACTII 360

Query: 367 FNTILAQLQTFSVQQGSLMDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKF 426
           FNTILAQLQTFSVQQGSLM+TQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKF
Sbjct: 361 FNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKF 420

Query: 427 TGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKRRRDSAVNLNQTISIFWITPQFLIFGLS 486
           TGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKRRRDSAVNLNQT+SIFWITPQFLIFGLS
Sbjct: 421 TGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKRRRDSAVNLNQTMSIFWITPQFLIFGLS 480

Query: 487 EMFTAVGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLH 546
           EMFTAVGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLH
Sbjct: 481 EMFTAVGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLH 540

Query: 547 DNNLNKDRLDLFYWLLAALSFLNFFNYLFWSTWFEEHPSLSQKPQHDNNLHHGESHHIYN 606
           DNNLNKDRLDLFYWLLAALSFLNF NYLFWSTWF+EHPSLSQK Q+D+N H GE + I+N
Sbjct: 541 DNNLNKDRLDLFYWLLAALSFLNFLNYLFWSTWFDEHPSLSQKLQNDDNPHQGEDYCIHN 600

Query: 607 FDSSKNNVADNVP 612
           F+SSKNNV D VP
Sbjct: 601 FNSSKNNVDDYVP 609

BLAST of Spg030004 vs. NCBI nr
Match: XP_023552004.1 (protein NRT1/ PTR FAMILY 4.3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1054.7 bits (2726), Expect = 3.1e-304
Identity = 543/616 (88.15%), Postives = 575/616 (93.34%), Query Frame = 0

Query: 7   IETTRRDHFKGE--IITHSLPSDQDLTVDWRGRPSSPVTHGGMRAAAFVLGLQAFEIMAI 66
           +E  RR  FKGE     +++PS +D+TVDWRGRPS+ V+HGGMRAAAFVLGLQAFEIMAI
Sbjct: 1   MEMERRRDFKGENNANANAMPS-EDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAI 60

Query: 67  AAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFV 126
           AAVGNNLITY+INEMHFSLSKSAN+VTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFV
Sbjct: 61  AAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFV 120

Query: 127 ELSGFILLSVQAHLPQLKPPQCNML-SEGEECVEAKGLKALIFFVALYLVALGSGCVKPN 186
           ELSGFILLSVQAH+PQLKPPQCNML S+G ECVEAKGLKALIFFVALYLVALGSGCVKPN
Sbjct: 121 ELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKPN 180

Query: 187 MIAHGADQFTQQNPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAA 246
           MIAHGADQF+ Q+PSQSK LSTYFNAAY AFSIGELIALTL+VWIQTHSGMDVGFGVSAA
Sbjct: 181 MIAHGADQFSPQSPSQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAA 240

Query: 247 LMAMGLISLVAGIFYYRNKPPQGAIFIPILQ--------RKQVCPSNPQMLHGSQTSNDK 306
           +MA+GLISLVAGIFYYRNKPPQG IF PILQ        RKQVCPSNP MLHGSQTSNDK
Sbjct: 241 VMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSNDK 300

Query: 307 VVALSSDSANLVPTQKFRFLDKACIKSQDGATKESPWRLCTPTQVEQVKILISVVPIFAC 366
            V LSS SANL+PTQKFRFL+KACIK+QD A KESPWRLCTP QVEQVKIL+SVVPIFAC
Sbjct: 301 -VGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360

Query: 367 TIIFNTILAQLQTFSVQQGSLMDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFA 426
           TI+FNTILAQLQTFSVQQGSLM+TQLTKSFHIPPASLQSIPYIMLI IVPLYDTFFVPFA
Sbjct: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA 420

Query: 427 RKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKRRRDSAVNLNQTISIFWITPQFLIF 486
           RKFTGHTSGISPLKRIGFGLFLS FSMV+AAIMEK+RRDSAVNLNQTISIFWITPQFLIF
Sbjct: 421 RKFTGHTSGISPLKRIGFGLFLSAFSMVAAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480

Query: 487 GLSEMFTAVGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 546
           GLSEMFTAVGLIEFFYKQS+KGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG
Sbjct: 481 GLSEMFTAVGLIEFFYKQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540

Query: 547 WLHDNNLNKDRLDLFYWLLAALSFLNFFNYLFWSTWFEEHPSLSQKPQHDNNLHHGESHH 606
           WLHDNNLNKDRLDLFYWLLAALSFLNF NYLFWS WF+EHPS+SQKPQH++N HHGE +H
Sbjct: 541 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600

Query: 607 IYNFDSSKNNVADNVP 612
           IYNF+SSKNNV D VP
Sbjct: 601 IYNFNSSKNNVDDYVP 614

BLAST of Spg030004 vs. NCBI nr
Match: XP_022929221.1 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1052.0 bits (2719), Expect = 2.0e-303
Identity = 542/618 (87.70%), Postives = 574/618 (92.88%), Query Frame = 0

Query: 7   IETTRRDHFKGE----IITHSLPSDQDLTVDWRGRPSSPVTHGGMRAAAFVLGLQAFEIM 66
           +E  RR  FKGE       +++PS +D+TVDWRGRPS+ V+HGGMRAAAFVLGLQAFEIM
Sbjct: 1   MEMERRRDFKGENNANANANAMPS-EDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIM 60

Query: 67  AIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFG 126
           AIAAVGNNLITY+INEMHFSLSKSAN+VTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFG
Sbjct: 61  AIAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFG 120

Query: 127 FVELSGFILLSVQAHLPQLKPPQCNML-SEGEECVEAKGLKALIFFVALYLVALGSGCVK 186
           FVELSGFILLSVQAH+PQLKPPQCNML S+G ECVEAKGLKALIFFVALYLVALGSGCVK
Sbjct: 121 FVELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVK 180

Query: 187 PNMIAHGADQFTQQNPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVS 246
           PNMIAHGADQF+ Q+P+QSK LSTYFNAAY AFSIGELIALTL+VWIQTHSGMDVGFGVS
Sbjct: 181 PNMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVS 240

Query: 247 AALMAMGLISLVAGIFYYRNKPPQGAIFIPILQ--------RKQVCPSNPQMLHGSQTSN 306
           AA+MA+GLISLVAGIFYYRNKPPQG IF PILQ        RKQVCPSNP MLHGSQTSN
Sbjct: 241 AAVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSN 300

Query: 307 DKVVALSSDSANLVPTQKFRFLDKACIKSQDGATKESPWRLCTPTQVEQVKILISVVPIF 366
           DK V LSS SANL+PTQKFRFL+KACIK+QD A KESPWRLCTP QVEQVKIL+SVVPIF
Sbjct: 301 DK-VGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIF 360

Query: 367 ACTIIFNTILAQLQTFSVQQGSLMDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVP 426
           ACTI+FNTILAQLQTFSVQQGSLM+TQLTKSFHIPPASLQSIPYIMLI IVPLYDTFFVP
Sbjct: 361 ACTIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVP 420

Query: 427 FARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKRRRDSAVNLNQTISIFWITPQFL 486
           FARKFTGHTSGISPLKRIGFGLFLS FSMVSAAIMEK+RRDSAVNLNQTISIFWITPQFL
Sbjct: 421 FARKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFL 480

Query: 487 IFGLSEMFTAVGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSN 546
           IFGLSEMFTAVGLIEFFY QS+KGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSN
Sbjct: 481 IFGLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSN 540

Query: 547 GGWLHDNNLNKDRLDLFYWLLAALSFLNFFNYLFWSTWFEEHPSLSQKPQHDNNLHHGES 606
           GGWLHDNNLNKDRLDLFYWLLAALSFLNF NYLFWS WF+EHPS+SQKPQH++N HHGE 
Sbjct: 541 GGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGED 600

Query: 607 HHIYNFDSSKNNVADNVP 612
           +HIYNF+SSKNNV D VP
Sbjct: 601 YHIYNFNSSKNNVDDYVP 616

BLAST of Spg030004 vs. NCBI nr
Match: XP_022993122.1 (protein NRT1/ PTR FAMILY 4.3-like [Cucurbita maxima])

HSP 1 Score: 1050.0 bits (2714), Expect = 7.7e-303
Identity = 547/604 (90.56%), Postives = 569/604 (94.21%), Query Frame = 0

Query: 1   MEMEMDIETTRRDHFKGEIITHSLPSDQDLTVDWRGRPSSPVTHGGMRAAAFVLGLQAFE 60
           MEMEMDIE  RRD       TH +PS +DLTVDWRGRPS+PV HGGMRAAAFVLGLQ+FE
Sbjct: 3   MEMEMDIE-RRRD-------THLMPS-EDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFE 62

Query: 61  IMAIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLI 120
           IMAIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLAL+GGFLSDSYLGSFWTMLI
Sbjct: 63  IMAIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLI 122

Query: 121 FGFVELSGFILLSVQAHLPQLKPPQCNMLSEGEECVEAKGLKALIFFVALYLVALGSGCV 180
           FGFVELSGFILLSVQAHLPQLKPPQCNMLSEG++CVEAKG+KALIFFVALYLVALGSGCV
Sbjct: 123 FGFVELSGFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCV 182

Query: 181 KPNMIAHGADQFTQQNPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGV 240
           KPNMIAHGADQFT Q+PSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGV
Sbjct: 183 KPNMIAHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGV 242

Query: 241 SAALMAMGLISLVAGIFYYRNKPPQGAIFIPILQ--------RKQVCPSNPQMLHGSQTS 300
           SAA+MAMGLISLV GIFYYRNKPPQGA+FIPILQ        RKQVCPSNPQMLHGSQTS
Sbjct: 243 SAAVMAMGLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTS 302

Query: 301 NDKVVALSSDSANLVPTQKFRFLDKACIKSQDGATKESPWRLCTPTQVEQVKILISVVPI 360
           N K VALSS SANL+PTQKFRFLDKACIKS DG T+ESPWRLCTPTQVEQVKILISVVPI
Sbjct: 303 NHK-VALSSHSANLIPTQKFRFLDKACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPI 362

Query: 361 FACTIIFNTILAQLQTFSVQQGSLMDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFV 420
           FACTIIFNTILAQLQTFSVQQGSLM+TQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFV
Sbjct: 363 FACTIIFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFV 422

Query: 421 PFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKRRRDSAVNLNQTISIFWITPQF 480
           PFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEK+RRDSAVN NQTISIFWITPQF
Sbjct: 423 PFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQF 482

Query: 481 LIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSS 540
           LIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKITSSS+
Sbjct: 483 LIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSST 542

Query: 541 NGGWLHDNNLNKDRLDLFYWLLAALSFLNFFNYLFWSTWFEEHPSLSQKPQHDNNLHHGE 597
           NGGWLHDNNLNKDRLDLFYWLLAALSFLNF NYLFWS+WF++ P LSQK Q D NLHHGE
Sbjct: 543 NGGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQD-NLHHGE 595

BLAST of Spg030004 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 6.6e-233
Identity = 414/553 (74.86%), Postives = 476/553 (86.08%), Query Frame = 0

Query: 28  QDLTVDWRGRPSSPVTHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYVINEMHFSLSKSA 87
           ++ +VDWRGRPS+P  HGGMRAA FVLGLQAFEIM IAAVGNNLITYVINEMHF LSK+A
Sbjct: 26  EEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAA 85

Query: 88  NVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCN 147
           N+VTNFVGT+F+ AL+GG+LSD++LGSFWT++IFGFVELSGFILLSVQAHLPQLKPP+CN
Sbjct: 86  NIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCN 145

Query: 148 MLSEGEECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFTQQNPSQSKRLSTYF 207
            L + + C EAKG KA+IFF+ALYLVALGSGCVKPNMIAHGADQF+Q +P QSKRLS+YF
Sbjct: 146 PLID-QTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYF 205

Query: 208 NAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAALMAMGLISLVAGIFYYRNKPPQGA 267
           NAAYFAFS+GELIALTL+VW+QTHSGMD+GFGVSAA M MG+ISLV+G  Y+RNK P+ +
Sbjct: 206 NAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRS 265

Query: 268 IFIP--------ILQRKQVCPSNPQMLHGSQTSNDKVVALSSDSANLVPTQKFRFLDKAC 327
           IF P        IL+RK   PS+P+MLHG     + VV     S+ L  T +FRFLDKAC
Sbjct: 266 IFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVANDVV----PSSTLPHTPRFRFLDKAC 325

Query: 328 IKSQDGATKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMDT 387
           IK QD  TKESPWRLCT TQVEQVK LIS+VPIFA TI+FNTILAQLQTFSVQQGS M+T
Sbjct: 326 IKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNT 385

Query: 388 QLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLST 447
           +L+ SFHIPPASLQ+IPYIMLIF+VPLYD+F VPFARK TGH SGI PL RIG GLFLST
Sbjct: 386 RLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLST 445

Query: 448 FSMVSAAIMEKRRRDSAVNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGMQ 507
           FSMVSAA++EK+RRDS+V   + +SIFWITPQFLIFG+SEMFTAVGLIEFFYKQS KGM+
Sbjct: 446 FSMVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGME 505

Query: 508 AFLTAMTYCSYSFGFYLSSLLVSMVNKITSSS-SNGGWLHDNNLNKDRLDLFYWLLAALS 567
           +FL A+TYCSYSFGFY SS+LVS+VNKITS+S  + GWL +N+LNKDRLDLFYWLLA LS
Sbjct: 506 SFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLS 565

Query: 568 FLNFFNYLFWSTW 572
            LNF +YLFWS W
Sbjct: 566 LLNFLSYLFWSRW 573

BLAST of Spg030004 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 5.3e-222
Identity = 411/613 (67.05%), Postives = 487/613 (79.45%), Query Frame = 0

Query: 5   MDIETTRRDHFKGEIITHSLPSDQDLTVDWRGRPSSPVTHGGMRAAAFVLGLQAFEIMAI 64
           MD+     +  +G I T S  S  DL VD+RGRP  P  HGG RAA FVLG QAFE+MAI
Sbjct: 1   MDVHDLSEEAKRGVIHT-SEESLDDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAI 60

Query: 65  AAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFV 124
           AAVGNNLITYV NEMHF LSKSAN+VTNF+GTVFLL+L+GGFLSDSYLGSF TML+FG +
Sbjct: 61  AAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVI 120

Query: 125 ELSGFILLSVQAHLPQLKPPQCNMLSEGEECVEAKGLKALIFFVALYLVALGSGCVKPNM 184
           E+SGFILLSVQAHLP+L+PP+CNM S    CVEA G KA   + AL LVALGSGC+KPN+
Sbjct: 121 EISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYKAATLYTALCLVALGSGCLKPNI 180

Query: 185 IAHGADQFTQQNPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAL 244
           I+HGA+QF +++    ++LS++FNAAYFAFS+G+LIALTL+VW+QTHSGMDVGFGVSAA+
Sbjct: 181 ISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAV 240

Query: 245 MAMGLISLVAGIFYYRNKPPQGAIFIPILQ--------RKQVCPSNPQMLHGSQTSNDKV 304
           MA G+ISLVAG  +YRNKPP G+IF PI Q        RKQ+CPSNP M+H  Q S D V
Sbjct: 241 MAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITKRKQICPSNPNMVH--QPSTDLV 300

Query: 305 VALSSDSANLVPTQKFRFLDKACIKSQDGATKESPWRLCTPTQVEQVKILISVVPIFACT 364
                    L+ + KFRFLDKACIK+Q G   ESPWRLCT  QV QVKIL+SV+PIFACT
Sbjct: 301 RV-----KPLLHSNKFRFLDKACIKTQ-GKAMESPWRLCTIEQVHQVKILLSVIPIFACT 360

Query: 365 IIFNTILAQLQTFSVQQGSLMDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFAR 424
           IIFNTILAQLQTFSVQQGS M+T +TK+F IPPASLQ+IPYI+LIF VPLY+TFFVP AR
Sbjct: 361 IIFNTILAQLQTFSVQQGSSMNTHITKTFQIPPASLQAIPYIILIFFVPLYETFFVPLAR 420

Query: 425 KFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKRRRDSAVNLNQTISIFWITPQFLIFG 484
           K TG+ SGISPL+RIG GLFL+TFSMV+AA++EK+RR+S +  N  +SIFWI PQFLIFG
Sbjct: 421 KLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQNVMLSIFWIAPQFLIFG 480

Query: 485 LSEMFTAVGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNG-- 544
           LSEMFTAVGL+EFFYKQS + MQ+FLTAMTYCSYSFGFYLSS+LVS VN++TSS+ +G  
Sbjct: 481 LSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTK 540

Query: 545 -GWLHDNNLNKDRLDLFYWLLAALSFLNFFNYLFWSTWFEEHPSLSQKPQHDNNLHHGES 604
            GWL DN+LNKDRLD FYWLLA+LSF+NFFNYLFWS W+   PS +      N+L   E+
Sbjct: 541 EGWLGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSRWYSCDPSATHHSAEVNSLEALEN 600

Query: 605 HHIYNFDSSKNNV 607
             I +  + K  +
Sbjct: 601 GEIKDSTTEKPRI 601

BLAST of Spg030004 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.9e-107
Identity = 230/566 (40.64%), Postives = 338/566 (59.72%), Query Frame = 0

Query: 33  DWRGRPSSPVTHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYVINEMHFSLSKSANVVTN 92
           DWR R +    HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH S SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 93  FVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSEG 152
           F+GT FLLAL+GGFLSD++  +F   LI   +E  G I+L++QA  P L PP C    + 
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSC----DS 134

Query: 153 EECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFTQQNPSQSKRLSTYFNAAYF 212
             C E  G KA + FV LYLVALG G +K ++ +HGA+QF +  P   K+ ST+FN   F
Sbjct: 135 PTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVF 194

Query: 213 AFSIGELIALTLVVWIQTHSGMDVGFGVSAALMAMGLISLVAGIFYYRNKPPQGAIFIPI 272
             + G L+A+T VVW++ + G + GFGVS   + + ++  ++G  +YRNK P G+    I
Sbjct: 195 CLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTI 254

Query: 273 LQRKQVCPSNPQMLHGSQTSNDKVVALSSDSANLV---------------PTQKFRFLDK 332
           L  K +  ++ +      +SN       S S + V               P Q+     +
Sbjct: 255 L--KVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPR 314

Query: 333 A----CIKSQDGATKESP-WRL--CTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFS 392
           A     +K  +GA  E P  RL  CT  QVE VKI++ ++PIFACTI+ N  LAQL TFS
Sbjct: 315 AQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFS 374

Query: 393 VQQGSLMDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKR 452
           VQQ + M+T++  S  IPPASL   P + ++ + P+YD   +PFARK T   +G++ L+R
Sbjct: 375 VQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQR 434

Query: 453 IGFGLFLSTFSMVSAAIMEKRRRDSA-----VNLNQT--ISIFWITPQFLIFGLSEMFTA 512
           IG GL LS  +M  AA++E +R+  A     ++  +T  ++  WI  Q+L  G +++FT 
Sbjct: 435 IGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTL 494

Query: 513 VGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLN 570
            GL+E+F+ ++   M++  T++++ S + G+YLSS++VS+VN IT SS N  WL   ++N
Sbjct: 495 AGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKSIN 554

BLAST of Spg030004 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 5.8e-104
Identity = 218/561 (38.86%), Postives = 336/561 (59.89%), Query Frame = 0

Query: 18  EIITHSLPSDQDLTVDWRGRPSSPVTHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYVIN 77
           E+  H   S  +   DWR + +    HGGM AA+FVL ++  E +A  A  +NL+ Y+ N
Sbjct: 2   EVEMHGDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKN 61

Query: 78  EMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAH 137
            MH SL++S++ VT F+ T FLLAL+GGFL+D++  +F   LI   +E  G ILL++QA 
Sbjct: 62  FMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQAR 121

Query: 138 LPQLKPPQCNMLSEGEECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFTQQNP 197
            P L PP C   S    C    G KA   FV LYLV+LG G +K ++ +HGA+QF +  P
Sbjct: 122 RPSLMPPPCKS-SAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTP 181

Query: 198 SQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAALMAMGLISLVAGIF 257
              K+ ST+FN   F  S G L+A+T VVWI+ + G + GFGVS   + + ++  + G  
Sbjct: 182 KGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSR 241

Query: 258 YYRNKPPQGAIFIPILQRKQVCPSNPQMLHGSQTSNDKVVA--LSSDSANLVPTQKFRFL 317
           +Y+NK P+G+   P+    +V  +   +   S+TS++   +  + S+     P+Q     
Sbjct: 242 FYKNKIPRGS---PLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSLTNS 301

Query: 318 DKACIKSQDGATKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGS 377
                K+ +G T    W  CT  QVE VKI++ ++PIF CTI+ N  LAQL T+SV Q +
Sbjct: 302 LTCLNKAIEGKTHHI-WLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAA 361

Query: 378 LMDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGL 437
            M+ ++  +F++P ASL   P + ++ + P YD   +PFARK T    GI+ L+RIG GL
Sbjct: 362 TMNRKIV-NFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGL 421

Query: 438 FLSTFSMVSAAIMEKRRRDSA-----VNLNQT--ISIFWITPQFLIFGLSEMFTAVGLIE 497
            LS  +M  AA++E +R+  A     ++  +T  I+  WI  Q+L  G +++FT  GL+E
Sbjct: 422 VLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLE 481

Query: 498 FFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLNKDRLD 557
           FF+ ++   M++  T++++ S + G+YLSS++V +VN++T S+    WL +  LN++RLD
Sbjct: 482 FFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGE-KLNRNRLD 541

Query: 558 LFYWLLAALSFLNFFNYLFWS 570
           LFYWL+  LS +NF +YLFW+
Sbjct: 542 LFYWLMCVLSVVNFLHYLFWA 555

BLAST of Spg030004 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.3e-100
Identity = 212/559 (37.92%), Postives = 336/559 (60.11%), Query Frame = 0

Query: 28  QDLTVDWRGRPSSPVTHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYVINEMHFSLSKSA 87
           QD TVD    P++    G  +A  F+LG +  E +A   +G NL+ Y+ + ++   + +A
Sbjct: 9   QDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAA 68

Query: 88  NVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCN 147
           N VTN+ GT ++  L+G F++D+YLG +WT+  F F+ +SG  LL++ A +P LKP  CN
Sbjct: 69  NNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCN 128

Query: 148 MLSEGEECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFTQQNPSQSKRLSTYF 207
                 +       +  +FFVALY++ALG+G +KP + + GADQF + + ++  + S++F
Sbjct: 129 -----ADTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 188

Query: 208 NAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAALMAMGLISLVAGIFYYRNKPPQGA 267
           N  YF+ ++G LIA T++VWIQ + G   GFGV    M + +     G  +YR + P G+
Sbjct: 189 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 248

Query: 268 IFIPILQ------RK--QVCPSNPQMLHGSQTSNDKVVALSSDSANLVPTQKFRFLDKAC 327
               I Q      RK     P +  +L   +T++D+  +    S  LV T   +F DKA 
Sbjct: 249 PLTRIFQVIVAAFRKISVKVPEDKSLLF--ETADDE--SNIKGSRKLVHTDNLKFFDKAA 308

Query: 328 IKSQDGATKE---SPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSL 387
           ++SQ  + K+   +PWRLC+ TQVE++K +I+++P++A  I+F T+ +Q+ T  V QG+ 
Sbjct: 309 VESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNT 368

Query: 388 MDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF 447
           MD  + K+F IP ASL     + ++F  P+YD F +P ARKFT +  G + L+R+G GL 
Sbjct: 369 MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 428

Query: 448 LSTFSMVSAAIMEKRRRDSAVNLNQ------TISIFWITPQFLIFGLSEMFTAVGLIEFF 507
           +S F+M++A ++E  R D     N        +SIFW  PQ+L+ G +E+FT +G +EFF
Sbjct: 429 VSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFF 488

Query: 508 YKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLNKDRLDLF 567
           Y Q+   M++  +A++  + + G YLS++LV++V KIT  +   GW+ D NLN+  LD F
Sbjct: 489 YDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPD-NLNRGHLDYF 548

Query: 568 YWLLAALSFLNFFNYLFWS 570
           ++LLA LSFLNF  YL+ S
Sbjct: 549 FYLLATLSFLNFLVYLWIS 557

BLAST of Spg030004 vs. ExPASy TrEMBL
Match: A0A6J1EMI3 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435878 PE=3 SV=1)

HSP 1 Score: 1052.0 bits (2719), Expect = 9.8e-304
Identity = 542/618 (87.70%), Postives = 574/618 (92.88%), Query Frame = 0

Query: 7   IETTRRDHFKGE----IITHSLPSDQDLTVDWRGRPSSPVTHGGMRAAAFVLGLQAFEIM 66
           +E  RR  FKGE       +++PS +D+TVDWRGRPS+ V+HGGMRAAAFVLGLQAFEIM
Sbjct: 1   MEMERRRDFKGENNANANANAMPS-EDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIM 60

Query: 67  AIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFG 126
           AIAAVGNNLITY+INEMHFSLSKSAN+VTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFG
Sbjct: 61  AIAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFG 120

Query: 127 FVELSGFILLSVQAHLPQLKPPQCNML-SEGEECVEAKGLKALIFFVALYLVALGSGCVK 186
           FVELSGFILLSVQAH+PQLKPPQCNML S+G ECVEAKGLKALIFFVALYLVALGSGCVK
Sbjct: 121 FVELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVK 180

Query: 187 PNMIAHGADQFTQQNPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVS 246
           PNMIAHGADQF+ Q+P+QSK LSTYFNAAY AFSIGELIALTL+VWIQTHSGMDVGFGVS
Sbjct: 181 PNMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVS 240

Query: 247 AALMAMGLISLVAGIFYYRNKPPQGAIFIPILQ--------RKQVCPSNPQMLHGSQTSN 306
           AA+MA+GLISLVAGIFYYRNKPPQG IF PILQ        RKQVCPSNP MLHGSQTSN
Sbjct: 241 AAVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSN 300

Query: 307 DKVVALSSDSANLVPTQKFRFLDKACIKSQDGATKESPWRLCTPTQVEQVKILISVVPIF 366
           DK V LSS SANL+PTQKFRFL+KACIK+QD A KESPWRLCTP QVEQVKIL+SVVPIF
Sbjct: 301 DK-VGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIF 360

Query: 367 ACTIIFNTILAQLQTFSVQQGSLMDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVP 426
           ACTI+FNTILAQLQTFSVQQGSLM+TQLTKSFHIPPASLQSIPYIMLI IVPLYDTFFVP
Sbjct: 361 ACTIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVP 420

Query: 427 FARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKRRRDSAVNLNQTISIFWITPQFL 486
           FARKFTGHTSGISPLKRIGFGLFLS FSMVSAAIMEK+RRDSAVNLNQTISIFWITPQFL
Sbjct: 421 FARKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFL 480

Query: 487 IFGLSEMFTAVGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSN 546
           IFGLSEMFTAVGLIEFFY QS+KGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSN
Sbjct: 481 IFGLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSN 540

Query: 547 GGWLHDNNLNKDRLDLFYWLLAALSFLNFFNYLFWSTWFEEHPSLSQKPQHDNNLHHGES 606
           GGWLHDNNLNKDRLDLFYWLLAALSFLNF NYLFWS WF+EHPS+SQKPQH++N HHGE 
Sbjct: 541 GGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGED 600

Query: 607 HHIYNFDSSKNNVADNVP 612
           +HIYNF+SSKNNV D VP
Sbjct: 601 YHIYNFNSSKNNVDDYVP 616

BLAST of Spg030004 vs. ExPASy TrEMBL
Match: A0A6J1JRW4 (protein NRT1/ PTR FAMILY 4.3-like OS=Cucurbita maxima OX=3661 GN=LOC111489235 PE=3 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 3.7e-303
Identity = 547/604 (90.56%), Postives = 569/604 (94.21%), Query Frame = 0

Query: 1   MEMEMDIETTRRDHFKGEIITHSLPSDQDLTVDWRGRPSSPVTHGGMRAAAFVLGLQAFE 60
           MEMEMDIE  RRD       TH +PS +DLTVDWRGRPS+PV HGGMRAAAFVLGLQ+FE
Sbjct: 3   MEMEMDIE-RRRD-------THLMPS-EDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFE 62

Query: 61  IMAIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLI 120
           IMAIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLAL+GGFLSDSYLGSFWTMLI
Sbjct: 63  IMAIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLI 122

Query: 121 FGFVELSGFILLSVQAHLPQLKPPQCNMLSEGEECVEAKGLKALIFFVALYLVALGSGCV 180
           FGFVELSGFILLSVQAHLPQLKPPQCNMLSEG++CVEAKG+KALIFFVALYLVALGSGCV
Sbjct: 123 FGFVELSGFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCV 182

Query: 181 KPNMIAHGADQFTQQNPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGV 240
           KPNMIAHGADQFT Q+PSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGV
Sbjct: 183 KPNMIAHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGV 242

Query: 241 SAALMAMGLISLVAGIFYYRNKPPQGAIFIPILQ--------RKQVCPSNPQMLHGSQTS 300
           SAA+MAMGLISLV GIFYYRNKPPQGA+FIPILQ        RKQVCPSNPQMLHGSQTS
Sbjct: 243 SAAVMAMGLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTS 302

Query: 301 NDKVVALSSDSANLVPTQKFRFLDKACIKSQDGATKESPWRLCTPTQVEQVKILISVVPI 360
           N K VALSS SANL+PTQKFRFLDKACIKS DG T+ESPWRLCTPTQVEQVKILISVVPI
Sbjct: 303 NHK-VALSSHSANLIPTQKFRFLDKACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPI 362

Query: 361 FACTIIFNTILAQLQTFSVQQGSLMDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFV 420
           FACTIIFNTILAQLQTFSVQQGSLM+TQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFV
Sbjct: 363 FACTIIFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFV 422

Query: 421 PFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKRRRDSAVNLNQTISIFWITPQF 480
           PFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEK+RRDSAVN NQTISIFWITPQF
Sbjct: 423 PFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQF 482

Query: 481 LIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSS 540
           LIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKITSSS+
Sbjct: 483 LIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSST 542

Query: 541 NGGWLHDNNLNKDRLDLFYWLLAALSFLNFFNYLFWSTWFEEHPSLSQKPQHDNNLHHGE 597
           NGGWLHDNNLNKDRLDLFYWLLAALSFLNF NYLFWS+WF++ P LSQK Q D NLHHGE
Sbjct: 543 NGGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQD-NLHHGE 595

BLAST of Spg030004 vs. ExPASy TrEMBL
Match: A0A6J1FR74 (protein NRT1/ PTR FAMILY 4.3-like OS=Cucurbita moschata OX=3662 GN=LOC111447459 PE=3 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 3.7e-303
Identity = 546/604 (90.40%), Postives = 568/604 (94.04%), Query Frame = 0

Query: 1   MEMEMDIETTRRDHFKGEIITHSLPSDQDLTVDWRGRPSSPVTHGGMRAAAFVLGLQAFE 60
           MEM+MD++  RR        TH +PS QDLTVDWRGRPS+PV HGGMRAAAFVLGLQ+FE
Sbjct: 3   MEMDMDMDIERRRD------THLMPS-QDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFE 62

Query: 61  IMAIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLI 120
           IMAIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLAL+GGFLSDSYLGSFWTMLI
Sbjct: 63  IMAIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLI 122

Query: 121 FGFVELSGFILLSVQAHLPQLKPPQCNMLSEGEECVEAKGLKALIFFVALYLVALGSGCV 180
           FGFVELSGFILLSVQAHLPQLKPPQCNMLSEG+ CVEAKG+KALIFFVALYLVALGSGCV
Sbjct: 123 FGFVELSGFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCV 182

Query: 181 KPNMIAHGADQFTQQNPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGV 240
           KPNMIAHGADQFT Q+PSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGV
Sbjct: 183 KPNMIAHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGV 242

Query: 241 SAALMAMGLISLVAGIFYYRNKPPQGAIFIPILQ--------RKQVCPSNPQMLHGSQTS 300
           SAA+MAMGLISLV GIFYYRNKPPQGAIFIPILQ        RKQVCPSNPQMLHGSQTS
Sbjct: 243 SAAVMAMGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTS 302

Query: 301 NDKVVALSSDSANLVPTQKFRFLDKACIKSQDGATKESPWRLCTPTQVEQVKILISVVPI 360
           N K VALSS SANL+PTQKFRFLDKACIKSQDG T+ESPWRLCT TQVEQVKILISVVPI
Sbjct: 303 NHK-VALSSHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPI 362

Query: 361 FACTIIFNTILAQLQTFSVQQGSLMDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFV 420
           FACTIIFNTILAQLQTFSVQQGSLM+TQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFV
Sbjct: 363 FACTIIFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFV 422

Query: 421 PFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKRRRDSAVNLNQTISIFWITPQF 480
           PFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEK+RRDSAVN NQTISIFWITPQF
Sbjct: 423 PFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQF 482

Query: 481 LIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSS 540
           LIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKITSSS+
Sbjct: 483 LIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSST 542

Query: 541 NGGWLHDNNLNKDRLDLFYWLLAALSFLNFFNYLFWSTWFEEHPSLSQKPQHDNNLHHGE 597
           NGGWLHDNNLNKDRLDLFYWLLAALSFLNF NYLFWS+WF++ PSLSQK Q D NLHHGE
Sbjct: 543 NGGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQD-NLHHGE 597

BLAST of Spg030004 vs. ExPASy TrEMBL
Match: A0A6J1JCZ7 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483371 PE=3 SV=1)

HSP 1 Score: 1048.9 bits (2711), Expect = 8.3e-303
Identity = 541/616 (87.82%), Postives = 572/616 (92.86%), Query Frame = 0

Query: 7   IETTRRDHFKGE--IITHSLPSDQDLTVDWRGRPSSPVTHGGMRAAAFVLGLQAFEIMAI 66
           +E  RR  FKGE     +++PS +D+TVDWRGRPS+ V+HGGMRAAAFVLGLQAFEIMAI
Sbjct: 1   MEMERRRDFKGENNANANAMPS-EDVTVDWRGRPSNSVSHGGMRAAAFVLGLQAFEIMAI 60

Query: 67  AAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFV 126
           AAVGNNLITY+INEMHFSLSKSAN+VTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFV
Sbjct: 61  AAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFV 120

Query: 127 ELSGFILLSVQAHLPQLKPPQCNML-SEGEECVEAKGLKALIFFVALYLVALGSGCVKPN 186
           ELSGFILLSVQAH+PQLKPPQCNML S+G ECVEAKGLKALIFFVALYLVALGSGCVKPN
Sbjct: 121 ELSGFILLSVQAHVPQLKPPQCNMLKSDGNECVEAKGLKALIFFVALYLVALGSGCVKPN 180

Query: 187 MIAHGADQFTQQNPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAA 246
           MIAHGADQF+ Q+PSQSK LSTYFNAAY AFSIGELIALTL+VWIQTHSGMDVGFGVSAA
Sbjct: 181 MIAHGADQFSPQSPSQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAA 240

Query: 247 LMAMGLISLVAGIFYYRNKPPQGAIFIPILQ--------RKQVCPSNPQMLHGSQTSNDK 306
           +MA+GLISLVAGIFYYRNKPPQG IF PILQ        RKQVCPSNP MLHGSQTSNDK
Sbjct: 241 VMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSNDK 300

Query: 307 VVALSSDSANLVPTQKFRFLDKACIKSQDGATKESPWRLCTPTQVEQVKILISVVPIFAC 366
            V LSS SANL+PTQKFRFL+KACIK+QD A KESPWRLCTP QVEQVKIL+SVVPIFAC
Sbjct: 301 -VGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360

Query: 367 TIIFNTILAQLQTFSVQQGSLMDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFA 426
           TI+FNTILAQLQTFSVQQGSLM+TQLTKS HIPPASLQSIPYIMLI IVPLYDTFFVPFA
Sbjct: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSLHIPPASLQSIPYIMLILIVPLYDTFFVPFA 420

Query: 427 RKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKRRRDSAVNLNQTISIFWITPQFLIF 486
           RK TGHTSGISPLKRIGFGLFLS FSMVSAAIMEK+RRDSAVNLNQTISIFWITPQFLIF
Sbjct: 421 RKITGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480

Query: 487 GLSEMFTAVGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 546
           GLSEMFTAVGLIEFFYKQS+KGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG
Sbjct: 481 GLSEMFTAVGLIEFFYKQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540

Query: 547 WLHDNNLNKDRLDLFYWLLAALSFLNFFNYLFWSTWFEEHPSLSQKPQHDNNLHHGESHH 606
           WLHDNNLNKDRLDLFYWLLAALSFLN  NYLFWS WF+EHPS+SQKPQH++N HHGE +H
Sbjct: 541 WLHDNNLNKDRLDLFYWLLAALSFLNLLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600

Query: 607 IYNFDSSKNNVADNVP 612
           IYNF+SSKNNV D VP
Sbjct: 601 IYNFNSSKNNVDDYVP 614

BLAST of Spg030004 vs. ExPASy TrEMBL
Match: A0A6J1C7K1 (protein NRT1/ PTR FAMILY 4.3-like OS=Momordica charantia OX=3673 GN=LOC111008146 PE=3 SV=1)

HSP 1 Score: 1046.6 bits (2705), Expect = 4.1e-302
Identity = 542/622 (87.14%), Postives = 570/622 (91.64%), Query Frame = 0

Query: 1   MEMEMDIETTRRDHFKGEIITHSLPSDQD-LTVDWRGRPSSPVTHGGMRAAAFVLGLQAF 60
           MEMEM+    RR  FKG+        D+D +TVDWRGRPS+P++HGGMRAAAFVLGLQAF
Sbjct: 1   MEMEME----RRKDFKGD-------EDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAF 60

Query: 61  EIMAIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTML 120
           EIMAIAAVGNNLITY INEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTML
Sbjct: 61  EIMAIAAVGNNLITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTML 120

Query: 121 IFGFVELSGFILLSVQAHLPQLKPPQCNMLSE-GEECVEAKGLKALIFFVALYLVALGSG 180
           +FGFVELSGFILLSVQAHLPQLKPPQCNMLSE GE C EAKGLKALIFFVALYLVALGSG
Sbjct: 121 VFGFVELSGFILLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSG 180

Query: 181 CVKPNMIAHGADQFT-QQNPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVG 240
           CVKPNMIAHGADQF+ Q NP  SK+LSTYFNAAYFAFS+GELIALTLVVWIQTHSGMDVG
Sbjct: 181 CVKPNMIAHGADQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVG 240

Query: 241 FGVSAALMAMGLISLVAGIFYYRNKPPQGAIFIPILQ--------RKQVCPSNPQMLHGS 300
           FGVSAA+MAMGLISLV GIFYYRNKPPQG IF PILQ        RKQVCPSNP MLHGS
Sbjct: 241 FGVSAAVMAMGLISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGS 300

Query: 301 QTSNDKVVALSSDSANLVPTQKFRFLDKACIKSQDGATKESPWRLCTPTQVEQVKILISV 360
           Q + D  VALSSDSANL+PTQKFRFLDKACIKSQDG TKESPWRLCTPTQVEQVKILISV
Sbjct: 301 QNNVDHKVALSSDSANLIPTQKFRFLDKACIKSQDGETKESPWRLCTPTQVEQVKILISV 360

Query: 361 VPIFACTIIFNTILAQLQTFSVQQGSLMDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDT 420
           VPIFACTIIFNTILAQLQTFSVQQGSLM+T+LTKSFHIPPASLQ+IPYIMLIFIVPLYDT
Sbjct: 361 VPIFACTIIFNTILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDT 420

Query: 421 FFVPFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKRRRDSAVNLNQTISIFWIT 480
           FFVPFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEK+RRDSA+NLNQT+SIFWIT
Sbjct: 421 FFVPFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWIT 480

Query: 481 PQFLIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITS 540
           PQFLIFGLSEMFTAVGLIEFFYKQS KGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKIT+
Sbjct: 481 PQFLIFGLSEMFTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITA 540

Query: 541 SSSNGGWLHDNNLNKDRLDLFYWLLAALSFLNFFNYLFWSTWFEEHPSLSQKPQHDNNLH 600
           +SSNGGWLHDNNLNKDRLDLFYWLLA LSFLNF NYLFWSTWF+EHPSLSQK  HD N H
Sbjct: 541 TSSNGGWLHDNNLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPH 600

Query: 601 HGESHHIYNFDSSKNNVADNVP 612
           HGE +H YNF+SSK N+ + VP
Sbjct: 601 HGEDYHSYNFNSSKTNIDEYVP 611

BLAST of Spg030004 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 808.1 bits (2086), Expect = 4.7e-234
Identity = 414/553 (74.86%), Postives = 476/553 (86.08%), Query Frame = 0

Query: 28  QDLTVDWRGRPSSPVTHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYVINEMHFSLSKSA 87
           ++ +VDWRGRPS+P  HGGMRAA FVLGLQAFEIM IAAVGNNLITYVINEMHF LSK+A
Sbjct: 26  EEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAA 85

Query: 88  NVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCN 147
           N+VTNFVGT+F+ AL+GG+LSD++LGSFWT++IFGFVELSGFILLSVQAHLPQLKPP+CN
Sbjct: 86  NIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCN 145

Query: 148 MLSEGEECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFTQQNPSQSKRLSTYF 207
            L + + C EAKG KA+IFF+ALYLVALGSGCVKPNMIAHGADQF+Q +P QSKRLS+YF
Sbjct: 146 PLID-QTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYF 205

Query: 208 NAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAALMAMGLISLVAGIFYYRNKPPQGA 267
           NAAYFAFS+GELIALTL+VW+QTHSGMD+GFGVSAA M MG+ISLV+G  Y+RNK P+ +
Sbjct: 206 NAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRS 265

Query: 268 IFIP--------ILQRKQVCPSNPQMLHGSQTSNDKVVALSSDSANLVPTQKFRFLDKAC 327
           IF P        IL+RK   PS+P+MLHG     + VV     S+ L  T +FRFLDKAC
Sbjct: 266 IFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVANDVV----PSSTLPHTPRFRFLDKAC 325

Query: 328 IKSQDGATKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMDT 387
           IK QD  TKESPWRLCT TQVEQVK LIS+VPIFA TI+FNTILAQLQTFSVQQGS M+T
Sbjct: 326 IKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNT 385

Query: 388 QLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLST 447
           +L+ SFHIPPASLQ+IPYIMLIF+VPLYD+F VPFARK TGH SGI PL RIG GLFLST
Sbjct: 386 RLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLST 445

Query: 448 FSMVSAAIMEKRRRDSAVNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGMQ 507
           FSMVSAA++EK+RRDS+V   + +SIFWITPQFLIFG+SEMFTAVGLIEFFYKQS KGM+
Sbjct: 446 FSMVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGME 505

Query: 508 AFLTAMTYCSYSFGFYLSSLLVSMVNKITSSS-SNGGWLHDNNLNKDRLDLFYWLLAALS 567
           +FL A+TYCSYSFGFY SS+LVS+VNKITS+S  + GWL +N+LNKDRLDLFYWLLA LS
Sbjct: 506 SFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLS 565

Query: 568 FLNFFNYLFWSTW 572
            LNF +YLFWS W
Sbjct: 566 LLNFLSYLFWSRW 573

BLAST of Spg030004 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 771.9 bits (1992), Expect = 3.8e-223
Identity = 411/613 (67.05%), Postives = 487/613 (79.45%), Query Frame = 0

Query: 5   MDIETTRRDHFKGEIITHSLPSDQDLTVDWRGRPSSPVTHGGMRAAAFVLGLQAFEIMAI 64
           MD+     +  +G I T S  S  DL VD+RGRP  P  HGG RAA FVLG QAFE+MAI
Sbjct: 1   MDVHDLSEEAKRGVIHT-SEESLDDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAI 60

Query: 65  AAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFV 124
           AAVGNNLITYV NEMHF LSKSAN+VTNF+GTVFLL+L+GGFLSDSYLGSF TML+FG +
Sbjct: 61  AAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVI 120

Query: 125 ELSGFILLSVQAHLPQLKPPQCNMLSEGEECVEAKGLKALIFFVALYLVALGSGCVKPNM 184
           E+SGFILLSVQAHLP+L+PP+CNM S    CVEA G KA   + AL LVALGSGC+KPN+
Sbjct: 121 EISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYKAATLYTALCLVALGSGCLKPNI 180

Query: 185 IAHGADQFTQQNPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAL 244
           I+HGA+QF +++    ++LS++FNAAYFAFS+G+LIALTL+VW+QTHSGMDVGFGVSAA+
Sbjct: 181 ISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAV 240

Query: 245 MAMGLISLVAGIFYYRNKPPQGAIFIPILQ--------RKQVCPSNPQMLHGSQTSNDKV 304
           MA G+ISLVAG  +YRNKPP G+IF PI Q        RKQ+CPSNP M+H  Q S D V
Sbjct: 241 MAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITKRKQICPSNPNMVH--QPSTDLV 300

Query: 305 VALSSDSANLVPTQKFRFLDKACIKSQDGATKESPWRLCTPTQVEQVKILISVVPIFACT 364
                    L+ + KFRFLDKACIK+Q G   ESPWRLCT  QV QVKIL+SV+PIFACT
Sbjct: 301 RV-----KPLLHSNKFRFLDKACIKTQ-GKAMESPWRLCTIEQVHQVKILLSVIPIFACT 360

Query: 365 IIFNTILAQLQTFSVQQGSLMDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFAR 424
           IIFNTILAQLQTFSVQQGS M+T +TK+F IPPASLQ+IPYI+LIF VPLY+TFFVP AR
Sbjct: 361 IIFNTILAQLQTFSVQQGSSMNTHITKTFQIPPASLQAIPYIILIFFVPLYETFFVPLAR 420

Query: 425 KFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKRRRDSAVNLNQTISIFWITPQFLIFG 484
           K TG+ SGISPL+RIG GLFL+TFSMV+AA++EK+RR+S +  N  +SIFWI PQFLIFG
Sbjct: 421 KLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQNVMLSIFWIAPQFLIFG 480

Query: 485 LSEMFTAVGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNG-- 544
           LSEMFTAVGL+EFFYKQS + MQ+FLTAMTYCSYSFGFYLSS+LVS VN++TSS+ +G  
Sbjct: 481 LSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTK 540

Query: 545 -GWLHDNNLNKDRLDLFYWLLAALSFLNFFNYLFWSTWFEEHPSLSQKPQHDNNLHHGES 604
            GWL DN+LNKDRLD FYWLLA+LSF+NFFNYLFWS W+   PS +      N+L   E+
Sbjct: 541 EGWLGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSRWYSCDPSATHHSAEVNSLEALEN 600

Query: 605 HHIYNFDSSKNNV 607
             I +  + K  +
Sbjct: 601 GEIKDSTTEKPRI 601

BLAST of Spg030004 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 391.3 bits (1004), Expect = 1.4e-108
Identity = 230/566 (40.64%), Postives = 338/566 (59.72%), Query Frame = 0

Query: 33  DWRGRPSSPVTHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYVINEMHFSLSKSANVVTN 92
           DWR R +    HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH S SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 93  FVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSEG 152
           F+GT FLLAL+GGFLSD++  +F   LI   +E  G I+L++QA  P L PP C    + 
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSC----DS 134

Query: 153 EECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFTQQNPSQSKRLSTYFNAAYF 212
             C E  G KA + FV LYLVALG G +K ++ +HGA+QF +  P   K+ ST+FN   F
Sbjct: 135 PTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVF 194

Query: 213 AFSIGELIALTLVVWIQTHSGMDVGFGVSAALMAMGLISLVAGIFYYRNKPPQGAIFIPI 272
             + G L+A+T VVW++ + G + GFGVS   + + ++  ++G  +YRNK P G+    I
Sbjct: 195 CLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTI 254

Query: 273 LQRKQVCPSNPQMLHGSQTSNDKVVALSSDSANLV---------------PTQKFRFLDK 332
           L  K +  ++ +      +SN       S S + V               P Q+     +
Sbjct: 255 L--KVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPR 314

Query: 333 A----CIKSQDGATKESP-WRL--CTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFS 392
           A     +K  +GA  E P  RL  CT  QVE VKI++ ++PIFACTI+ N  LAQL TFS
Sbjct: 315 AQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFS 374

Query: 393 VQQGSLMDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKR 452
           VQQ + M+T++  S  IPPASL   P + ++ + P+YD   +PFARK T   +G++ L+R
Sbjct: 375 VQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQR 434

Query: 453 IGFGLFLSTFSMVSAAIMEKRRRDSA-----VNLNQT--ISIFWITPQFLIFGLSEMFTA 512
           IG GL LS  +M  AA++E +R+  A     ++  +T  ++  WI  Q+L  G +++FT 
Sbjct: 435 IGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTL 494

Query: 513 VGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLN 570
            GL+E+F+ ++   M++  T++++ S + G+YLSS++VS+VN IT SS N  WL   ++N
Sbjct: 495 AGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKSIN 554

BLAST of Spg030004 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 379.8 bits (974), Expect = 4.1e-105
Identity = 218/561 (38.86%), Postives = 336/561 (59.89%), Query Frame = 0

Query: 18  EIITHSLPSDQDLTVDWRGRPSSPVTHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYVIN 77
           E+  H   S  +   DWR + +    HGGM AA+FVL ++  E +A  A  +NL+ Y+ N
Sbjct: 2   EVEMHGDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKN 61

Query: 78  EMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAH 137
            MH SL++S++ VT F+ T FLLAL+GGFL+D++  +F   LI   +E  G ILL++QA 
Sbjct: 62  FMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQAR 121

Query: 138 LPQLKPPQCNMLSEGEECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFTQQNP 197
            P L PP C   S    C    G KA   FV LYLV+LG G +K ++ +HGA+QF +  P
Sbjct: 122 RPSLMPPPCKS-SAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTP 181

Query: 198 SQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAALMAMGLISLVAGIF 257
              K+ ST+FN   F  S G L+A+T VVWI+ + G + GFGVS   + + ++  + G  
Sbjct: 182 KGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSR 241

Query: 258 YYRNKPPQGAIFIPILQRKQVCPSNPQMLHGSQTSNDKVVA--LSSDSANLVPTQKFRFL 317
           +Y+NK P+G+   P+    +V  +   +   S+TS++   +  + S+     P+Q     
Sbjct: 242 FYKNKIPRGS---PLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSLTNS 301

Query: 318 DKACIKSQDGATKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGS 377
                K+ +G T    W  CT  QVE VKI++ ++PIF CTI+ N  LAQL T+SV Q +
Sbjct: 302 LTCLNKAIEGKTHHI-WLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAA 361

Query: 378 LMDTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGL 437
            M+ ++  +F++P ASL   P + ++ + P YD   +PFARK T    GI+ L+RIG GL
Sbjct: 362 TMNRKIV-NFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGL 421

Query: 438 FLSTFSMVSAAIMEKRRRDSA-----VNLNQT--ISIFWITPQFLIFGLSEMFTAVGLIE 497
            LS  +M  AA++E +R+  A     ++  +T  I+  WI  Q+L  G +++FT  GL+E
Sbjct: 422 VLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLE 481

Query: 498 FFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLNKDRLD 557
           FF+ ++   M++  T++++ S + G+YLSS++V +VN++T S+    WL +  LN++RLD
Sbjct: 482 FFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGE-KLNRNRLD 541

Query: 558 LFYWLLAALSFLNFFNYLFWS 570
           LFYWL+  LS +NF +YLFW+
Sbjct: 542 LFYWLMCVLSVVNFLHYLFWA 555

BLAST of Spg030004 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 377.9 bits (969), Expect = 1.6e-104
Identity = 215/546 (39.38%), Postives = 331/546 (60.62%), Query Frame = 0

Query: 33  DWRGRPSSPVTHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYVINEMHFSLSKSANVVTN 92
           DWR + +    HGGM AA+FVL ++  E +A  A  +NL+ Y+ N MH SL++S++ VT 
Sbjct: 13  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 72

Query: 93  FVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSEG 152
           F+ T FLLAL+GGFL+D++  +F   LI   +E  G ILL++QA  P L PP C   S  
Sbjct: 73  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKS-SAA 132

Query: 153 EECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFTQQNPSQSKRLSTYFNAAYF 212
             C    G KA   FV LYLV+LG G +K ++ +HGA+QF +  P   K+ ST+FN   F
Sbjct: 133 LRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVF 192

Query: 213 AFSIGELIALTLVVWIQTHSGMDVGFGVSAALMAMGLISLVAGIFYYRNKPPQGAIFIPI 272
             S G L+A+T VVWI+ + G + GFGVS   + + ++  + G  +Y+NK P+G+   P+
Sbjct: 193 CLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGS---PL 252

Query: 273 LQRKQVCPSNPQMLHGSQTSNDKVVA--LSSDSANLVPTQKFRFLDKACIKSQDGATKES 332
               +V  +   +   S+TS++   +  + S+     P+Q          K+ +G T   
Sbjct: 253 TTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAIEGKTHHI 312

Query: 333 PWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMDTQLTKSFHIPPA 392
            W  CT  QVE VKI++ ++PIF CTI+ N  LAQL T+SV Q + M+ ++  +F++P A
Sbjct: 313 -WLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIV-NFNVPSA 372

Query: 393 SLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEK 452
           SL   P + ++ + P YD   +PFARK T    GI+ L+RIG GL LS  +M  AA++E 
Sbjct: 373 SLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVEL 432

Query: 453 RRRDSA-----VNLNQT--ISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGMQAFLT 512
           +R+  A     ++  +T  I+  WI  Q+L  G +++FT  GL+EFF+ ++   M++  T
Sbjct: 433 KRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLAT 492

Query: 513 AMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLNKDRLDLFYWLLAALSFLNFF 570
           ++++ S + G+YLSS++V +VN++T S+    WL +  LN++RLDLFYWL+  LS +NF 
Sbjct: 493 SLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGE-KLNRNRLDLFYWLMCVLSVVNFL 551