Spg029862 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg029862
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionMembrane-associated kinase regulator 5
Locationscaffold6: 8509132 .. 8510625 (+)
RNA-Seq ExpressionSpg029862
SyntenySpg029862
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAGCTTCAGCTTGCTCAAATACTGGAGAGGGGGCGGCGGGGCTGCTGCTGCTGCTGCAGCTGCTGATTTTGCTTTCAACAGCAATTCCTCCGCCGGCACCGCCACTGTTGTCACCGCCGTCGCGCAGAACCAGGCCGACACTGATGAGGAGGAGGATGGCGATGATGGGCCTTTTTTTGATTTGGAGTTTGCTGTTCCTGATGAGGATGAGGGTGGTCGGAAACAGGGGGGAAATGGCGAGGGGAGAAACTGTGTTGATGGAAGTGAGGAGGAGAATGAGGAAGATGAAGAAGAAGAAGAAGAAGATGGGGATTCTGATGGGGAGAGAGAGTTCAACTTCACGCTCTCATCTGGCTCCAGCAATGAACATTCTGATGCCAATCTCACGTTTTCTCCTTCGGATGACTTGTTTTTCAAGGGAAAGCTTGTCCCTGTTGAAGCTTCCTCCAATGGCGGTTCTGAGCCGAACACCAGGCCGCAGCTTCGTGTCACATTGTTGAAATCAGCCGCCAAGTTTAGGGTATTCATGTCGGGTCTCAAGAAATCGAAATTAAATGGGGAGAAGAAAACAGAAACGGATGGATCTGTTGGGGAAACGACTAAAAGCGAGACAAAAGAAGAGGCGCCGGAAAAACCACCGGAGAAGAACGAGAACCAACCGGAGAAAAACCAGAACCAAACGAAAAGCAAGTTTTTTGCTGTGAAATTTAAGGTGGAGGAAGTTCCGATGATGTCTCTGTTTACCAGAGACAACAGTTCGAGAGGCTCCGGTGGAATTACCACCACCACCAACAACAACAAACCACAGAAACAAAGCTCCGATGAATCGACTTCTGAGGAAAATCGATTCTCAAAAGATGTAATAATGCAAAAGTACTTGAAAATGGTGAAGCCTCTGTACATTCGGGTTTCAAGGAGATACGGCGAGAAGTTCAGGTTATCCGGGCAGCTGATTCTGGGGGCAGCCGGAGCAAAGTCCGGCGCGCCGCCGTCAATAGCAGCGCCACCCACGTCCCCCCCGGTGGAGAAGGCCGTGACAGAGACAGAAGCTGTGCAGGTGCAAGCGCCAACAACCACCGCCAACGCCAAGGGCCGGAACATGCCGGCGAAGCTAAGAGTGGTTTGCAAGCATTTGGGGAAAAGTCGGTCAGCTTCCTCCGCCGTAGCGGCGGCGCCACCGGGGCAGGCGCCATCGAGAAGGGACGATTCCCTTCTGCAGCAACAAGACGGAATCCAGAGCGCCATTCTTCACTGCAAGAGATCCTTCAACGCTTCTCGAGGTAAAAAGAAGAAACTGTTCACTATTCTGTTACTTGTATTAAAAGTTTCACCTTTTTTCCTCAACTTCATTAATGGATCATTTCTTTTCTGTTTCTGCTCTGCTTACACCAAAACATCAACAGATGCAGAGTCTTCCCTGCTGTCAAGGTCAGTAAGCGACACTCTGCGTGAGAAACCTTCAGAATTAACAGAAAAAGCCATTGGATGA

mRNA sequence

ATGGAAAGCTTCAGCTTGCTCAAATACTGGAGAGGGGGCGGCGGGGCTGCTGCTGCTGCTGCAGCTGCTGATTTTGCTTTCAACAGCAATTCCTCCGCCGGCACCGCCACTGTTGTCACCGCCGTCGCGCAGAACCAGGCCGACACTGATGAGGAGGAGGATGGCGATGATGGGCCTTTTTTTGATTTGGAGTTTGCTGTTCCTGATGAGGATGAGGGTGGTCGGAAACAGGGGGGAAATGGCGAGGGGAGAAACTGTGTTGATGGAAGTGAGGAGGAGAATGAGGAAGATGAAGAAGAAGAAGAAGAAGATGGGGATTCTGATGGGGAGAGAGAGTTCAACTTCACGCTCTCATCTGGCTCCAGCAATGAACATTCTGATGCCAATCTCACGTTTTCTCCTTCGGATGACTTGTTTTTCAAGGGAAAGCTTGTCCCTGTTGAAGCTTCCTCCAATGGCGGTTCTGAGCCGAACACCAGGCCGCAGCTTCGTGTCACATTGTTGAAATCAGCCGCCAAGTTTAGGGTATTCATGTCGGGTCTCAAGAAATCGAAATTAAATGGGGAGAAGAAAACAGAAACGGATGGATCTGTTGGGGAAACGACTAAAAGCGAGACAAAAGAAGAGGCGCCGGAAAAACCACCGGAGAAGAACGAGAACCAACCGGAGAAAAACCAGAACCAAACGAAAAGCAAGTTTTTTGCTGTGAAATTTAAGGTGGAGGAAGTTCCGATGATGTCTCTGTTTACCAGAGACAACAGTTCGAGAGGCTCCGGTGGAATTACCACCACCACCAACAACAACAAACCACAGAAACAAAGCTCCGATGAATCGACTTCTGAGGAAAATCGATTCTCAAAAGATGTAATAATGCAAAAGTACTTGAAAATGGTGAAGCCTCTGTACATTCGGGTTTCAAGGAGATACGGCGAGAAGTTCAGGTTATCCGGGCAGCTGATTCTGGGGGCAGCCGGAGCAAAGTCCGGCGCGCCGCCGTCAATAGCAGCGCCACCCACGTCCCCCCCGGTGGAGAAGGCCGTGACAGAGACAGAAGCTGTGCAGGTGCAAGCGCCAACAACCACCGCCAACGCCAAGGGCCGGAACATGCCGGCGAAGCTAAGAGTGGTTTGCAAGCATTTGGGGAAAAGTCGGTCAGCTTCCTCCGCCGTAGCGGCGGCGCCACCGGGGCAGGCGCCATCGAGAAGGGACGATTCCCTTCTGCAGCAACAAGACGGAATCCAGAGCGCCATTCTTCACTGCAAGAGATCCTTCAACGCTTCTCGAGGTAAAAAGAAGAAACTGTTCACTATTCTGTTACTTGTATTAAAAGTTTCACCTTTTTTCCTCAACTTCATTAATGGATCATTTCTTTTCTGTTTCTGCTCTGCTTACACCAAAACATCAACAGATGCAGAGTCTTCCCTGCTGTCAAGGTCAGTAAGCGACACTCTGCGTGAGAAACCTTCAGAATTAACAGAAAAAGCCATTGGATGA

Coding sequence (CDS)

ATGGAAAGCTTCAGCTTGCTCAAATACTGGAGAGGGGGCGGCGGGGCTGCTGCTGCTGCTGCAGCTGCTGATTTTGCTTTCAACAGCAATTCCTCCGCCGGCACCGCCACTGTTGTCACCGCCGTCGCGCAGAACCAGGCCGACACTGATGAGGAGGAGGATGGCGATGATGGGCCTTTTTTTGATTTGGAGTTTGCTGTTCCTGATGAGGATGAGGGTGGTCGGAAACAGGGGGGAAATGGCGAGGGGAGAAACTGTGTTGATGGAAGTGAGGAGGAGAATGAGGAAGATGAAGAAGAAGAAGAAGAAGATGGGGATTCTGATGGGGAGAGAGAGTTCAACTTCACGCTCTCATCTGGCTCCAGCAATGAACATTCTGATGCCAATCTCACGTTTTCTCCTTCGGATGACTTGTTTTTCAAGGGAAAGCTTGTCCCTGTTGAAGCTTCCTCCAATGGCGGTTCTGAGCCGAACACCAGGCCGCAGCTTCGTGTCACATTGTTGAAATCAGCCGCCAAGTTTAGGGTATTCATGTCGGGTCTCAAGAAATCGAAATTAAATGGGGAGAAGAAAACAGAAACGGATGGATCTGTTGGGGAAACGACTAAAAGCGAGACAAAAGAAGAGGCGCCGGAAAAACCACCGGAGAAGAACGAGAACCAACCGGAGAAAAACCAGAACCAAACGAAAAGCAAGTTTTTTGCTGTGAAATTTAAGGTGGAGGAAGTTCCGATGATGTCTCTGTTTACCAGAGACAACAGTTCGAGAGGCTCCGGTGGAATTACCACCACCACCAACAACAACAAACCACAGAAACAAAGCTCCGATGAATCGACTTCTGAGGAAAATCGATTCTCAAAAGATGTAATAATGCAAAAGTACTTGAAAATGGTGAAGCCTCTGTACATTCGGGTTTCAAGGAGATACGGCGAGAAGTTCAGGTTATCCGGGCAGCTGATTCTGGGGGCAGCCGGAGCAAAGTCCGGCGCGCCGCCGTCAATAGCAGCGCCACCCACGTCCCCCCCGGTGGAGAAGGCCGTGACAGAGACAGAAGCTGTGCAGGTGCAAGCGCCAACAACCACCGCCAACGCCAAGGGCCGGAACATGCCGGCGAAGCTAAGAGTGGTTTGCAAGCATTTGGGGAAAAGTCGGTCAGCTTCCTCCGCCGTAGCGGCGGCGCCACCGGGGCAGGCGCCATCGAGAAGGGACGATTCCCTTCTGCAGCAACAAGACGGAATCCAGAGCGCCATTCTTCACTGCAAGAGATCCTTCAACGCTTCTCGAGGTAAAAAGAAGAAACTGTTCACTATTCTGTTACTTGTATTAAAAGTTTCACCTTTTTTCCTCAACTTCATTAATGGATCATTTCTTTTCTGTTTCTGCTCTGCTTACACCAAAACATCAACAGATGCAGAGTCTTCCCTGCTGTCAAGGTCAGTAAGCGACACTCTGCGTGAGAAACCTTCAGAATTAACAGAAAAAGCCATTGGATGA

Protein sequence

MESFSLLKYWRGGGGAAAAAAAADFAFNSNSSAGTATVVTAVAQNQADTDEEEDGDDGPFFDLEFAVPDEDEGGRKQGGNGEGRNCVDGSEEENEEDEEEEEEDGDSDGEREFNFTLSSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNTRPQLRVTLLKSAAKFRVFMSGLKKSKLNGEKKTETDGSVGETTKSETKEEAPEKPPEKNENQPEKNQNQTKSKFFAVKFKVEEVPMMSLFTRDNSSRGSGGITTTTNNNKPQKQSSDESTSEENRFSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGAPPSIAAPPTSPPVEKAVTETEAVQVQAPTTTANAKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQSAILHCKRSFNASRGKKKKLFTILLLVLKVSPFFLNFINGSFLFCFCSAYTKTSTDAESSLLSRSVSDTLREKPSELTEKAIG
Homology
BLAST of Spg029862 vs. NCBI nr
Match: KAG6600544.1 (putative membrane-associated kinase regulator 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031183.1 putative membrane-associated kinase regulator 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 679.1 bits (1751), Expect = 2.9e-191
Identity = 400/510 (78.43%), Postives = 422/510 (82.75%), Query Frame = 0

Query: 1   MESFSLLKYWR---GGGGAAAAAAAADFAFNSNSSAGTATVVTAVAQNQADTDEEEDGDD 60
           MESFSLLKYWR   GGGG A+A+AAADFAF+S SSA TATVVTAVA+NQA+TDE+ED DD
Sbjct: 1   MESFSLLKYWRGGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDD 60

Query: 61  GPFFDLEFAVPDEDEGGRKQG------GNGEGRNCVDGSEEENEEDEEEEEEDGDSDGER 120
           GPFFDLEFAVPDEDE GRKQG      G+G+GRNC DGSEEEN++DEEEEEEDGDSDGER
Sbjct: 61  GPFFDLEFAVPDEDE-GRKQGGDGDGDGDGDGRNCDDGSEEENDDDEEEEEEDGDSDGER 120

Query: 121 EFNFTLSSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNTRPQLRVTLLKSA 180
           EF+FTLSSGS+NEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPN++PQLRVTLLKSA
Sbjct: 121 EFDFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSA 180

Query: 181 AKFRVFMSGLKKSKLNGEKKTETDGSVGETTKSETKEEAPEKPPEKNENQPEKNQNQTKS 240
           AKFRVFMSGLKKSK NGEKKTE DGSVGE TKSET E  PE PPEKNENQ +K+QNQTKS
Sbjct: 181 AKFRVFMSGLKKSKSNGEKKTEVDGSVGEPTKSETTELTPENPPEKNENQSDKSQNQTKS 240

Query: 241 KFFAVKFKVEEVPMMSLFTRDNSSRGSGGITTTTNN----NKPQKQSSDESTSEENRFSK 300
           K FAVK KVEEVP+MSLFTRDNSSRGS G TTTTNN    NK QKQSSDE TSEENRFSK
Sbjct: 241 KLFAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTNNNNNSNKAQKQSSDELTSEENRFSK 300

Query: 301 DVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGAPPSIAAPPTSPPVEKAV 360
           DVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAA AKSGA P+IAAPPTSPPVEK V
Sbjct: 301 DVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVEKTV 360

Query: 361 TETEAVQVQAPTTTANAKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLL 420
           T+TE V+VQA TTT N KGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLL
Sbjct: 361 TDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLL 420

Query: 421 QQQDGIQSAILHCKRSFNASRGKKKKLFTILLLVLKVSPFFLNFINGSFLFCFCSAYTKT 480
           QQQDGIQSAILHCKRSFNASR                                       
Sbjct: 421 QQQDGIQSAILHCKRSFNASR--------------------------------------- 462

Query: 481 STDAESSLLSRSVSDTLREKPSELTEKAIG 498
             D+ESSLLS      LREKPS+ TEKAIG
Sbjct: 481 --DSESSLLS------LREKPSDFTEKAIG 462

BLAST of Spg029862 vs. NCBI nr
Match: XP_023529752.1 (probable membrane-associated kinase regulator 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 676.8 bits (1745), Expect = 1.4e-190
Identity = 398/503 (79.13%), Postives = 420/503 (83.50%), Query Frame = 0

Query: 1   MESFSLLKYWR--GGGGAAAAAAAADFAFNSNSSAGTATVVTAVAQNQADTDEEEDGDDG 60
           MESFSLLKYWR  GGGG A+A+AAADFAF+S SSA TATVVTAVA+NQA+TDE+ED DDG
Sbjct: 1   MESFSLLKYWRGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG 60

Query: 61  PFFDLEFAVPDEDEGGRKQGGNGEGRNCVDGSEEENEED-EEEEEEDGDSDGEREFNFTL 120
           PFFDLEFAVPDEDE GRKQGG+G+ RNC DGSEEEN+ED EEEEEEDGDSDGEREF+FTL
Sbjct: 61  PFFDLEFAVPDEDE-GRKQGGDGDDRNCDDGSEEENDEDEEEEEEEDGDSDGEREFDFTL 120

Query: 121 SSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNTRPQLRVTLLKSAAKFRVF 180
           SSGS+NEHSDANLTFSPSDDLFFKG+L+PVEASSNGGSEPN++PQLRVTLLKSAAKFRVF
Sbjct: 121 SSGSTNEHSDANLTFSPSDDLFFKGRLLPVEASSNGGSEPNSKPQLRVTLLKSAAKFRVF 180

Query: 181 MSGLKKSKLNGEKKTETDGSVGETTKSETKEEAPEKPPEKNENQPEKNQNQTKSKFFAVK 240
           MSGLKKSK NGEKKTE DGSVGE TK ET E  PE PPEKNENQ +K+QNQTKSK FAVK
Sbjct: 181 MSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKLFAVK 240

Query: 241 FKVEEVPMMSLFTRDNSSRGSGGITTTT---NNNKPQKQSSDESTSEENRFSKDVIMQKY 300
            KVEEVP+MSLFTRDNSSRGS G TTTT   NNNK QKQSSDE TSEE RFSKDVIMQKY
Sbjct: 241 LKVEEVPIMSLFTRDNSSRGSAGTTTTTTTNNNNKAQKQSSDELTSEETRFSKDVIMQKY 300

Query: 301 LKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGAPPSIAAPPTSPPVEKAVTETEAVQ 360
           LKMVKPLYIRVSRRYGEKFRLSGQLILGAA AKSGA PSIAAPPTSPPVEKAVT+TE V+
Sbjct: 301 LKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPSIAAPPTSPPVEKAVTDTELVE 360

Query: 361 VQAPTTTANAKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQ 420
           VQA TTT N KGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQ
Sbjct: 361 VQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQ 420

Query: 421 SAILHCKRSFNASRGKKKKLFTILLLVLKVSPFFLNFINGSFLFCFCSAYTKTSTDAESS 480
           SAILHCKRSFNASR                                         D+ESS
Sbjct: 421 SAILHCKRSFNASR-----------------------------------------DSESS 455

Query: 481 LLSRSVSDTLREKPSELTEKAIG 498
           LLS      LREKPS+ TEKAIG
Sbjct: 481 LLS------LREKPSDFTEKAIG 455

BLAST of Spg029862 vs. NCBI nr
Match: XP_022942302.1 (probable membrane-associated kinase regulator 2 [Cucurbita moschata])

HSP 1 Score: 670.6 bits (1729), Expect = 1.0e-188
Identity = 398/512 (77.73%), Postives = 421/512 (82.23%), Query Frame = 0

Query: 1   MESFSLLKYWR--GGGGAAAAAAAADFAFNSNSSAGTATVVTAVAQNQADTDEEEDGDDG 60
           MESFSLLKYWR  GGGG A+A+AAADFAF+S SSA TATVVTAVA+NQA+TDE+ED DDG
Sbjct: 1   MESFSLLKYWRGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG 60

Query: 61  PFFDLEFAVPDEDEGGRKQG----GNGEGRNCVDGSEEENEED--EEEEEEDGDSDGERE 120
           PFFDLEFAVPDEDE GRKQG    G+G+GRNC DGSEEEN++D  EEEEEEDGDSDGERE
Sbjct: 61  PFFDLEFAVPDEDE-GRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGERE 120

Query: 121 FNFTLSSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNTRPQLRVTLLKSAA 180
           F+FTLSSGS+NEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPN++PQLRVTLLKSAA
Sbjct: 121 FDFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAA 180

Query: 181 KFRVFMSGLKKSKLNGEKKTETDGSVGETTKSETKEEAPEKPPEKNENQPEKNQNQTKSK 240
           KFRVFMSGLKKSK NGEKKTE DGSVGE TK ET E  PE PPEKNENQ +K+QNQTKSK
Sbjct: 181 KFRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSK 240

Query: 241 FFAVKFKVEEVPMMSLFTRDNSSRGSGGITTTT-------NNNKPQKQSSDESTSEENRF 300
            FAVK KVEEVP+MSLFTRDNSSRGS G TTTT       N+NK QKQSSDE TSEENRF
Sbjct: 241 LFAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSDELTSEENRF 300

Query: 301 SKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGAPPSIAAPPTSPPVEK 360
           SKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAA AKSGA P+IAAPPTSPPVEK
Sbjct: 301 SKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVEK 360

Query: 361 AVTETEAVQVQAPTTTANAKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDS 420
            VT+TE V+VQA TTT N KGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDS
Sbjct: 361 TVTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDS 420

Query: 421 LLQQQDGIQSAILHCKRSFNASRGKKKKLFTILLLVLKVSPFFLNFINGSFLFCFCSAYT 480
           LLQQQDGIQSAILHCKRSFNASR                                     
Sbjct: 421 LLQQQDGIQSAILHCKRSFNASR------------------------------------- 464

Query: 481 KTSTDAESSLLSRSVSDTLREKPSELTEKAIG 498
               D+ESSLLS      LREKPS+ TEKAIG
Sbjct: 481 ----DSESSLLS------LREKPSDFTEKAIG 464

BLAST of Spg029862 vs. NCBI nr
Match: XP_022980889.1 (probable membrane-associated kinase regulator 2 [Cucurbita maxima])

HSP 1 Score: 667.2 bits (1720), Expect = 1.1e-187
Identity = 393/502 (78.29%), Postives = 414/502 (82.47%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGAAA-AAAAADFAFNSNSSAGTATVVTAVAQNQADTDEEEDGDDGP 60
           MESFSLLKYWRGGGG  A A+AAADFAF+S SSA TATVVTAVA+NQA+TDE+ED DDGP
Sbjct: 1   MESFSLLKYWRGGGGGGAPASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDGP 60

Query: 61  FFDLEFAVPDEDEGGRKQGGNGEGRNCVDGSEEENEEDEEEEEEDGDSDGEREFNFTLSS 120
           FFDLEFAVP+EDE GRKQGG+G+GRNC DGSEEEN     ++EEDGDSDGEREF+FTLSS
Sbjct: 61  FFDLEFAVPEEDE-GRKQGGDGDGRNCDDGSEEEN-----DDEEDGDSDGEREFDFTLSS 120

Query: 121 GSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNTRPQLRVTLLKSAAKFRVFMS 180
           GS+NEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPN++PQLRVTLLKSAAKFRVFMS
Sbjct: 121 GSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAKFRVFMS 180

Query: 181 GLKKSKLNGEKKTETDGSVGETTKSETKEEAPEKPPEKNENQPEKNQNQTKSKFFAVKFK 240
           GLKKSK NGEKKTETDGSVGE TK ET E  PE PPEKNENQ +K+QNQTKSK FAVK K
Sbjct: 181 GLKKSKSNGEKKTETDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKLFAVKLK 240

Query: 241 VEEVPMMSLFTRDNSSRGSGGITTTT-----NNNKPQKQSSDESTSEENRFSKDVIMQKY 300
           VEEVP+MSLFTRDNSSRGS G TTTT     NNNK QKQSSDE TSEENRFSKDVIMQKY
Sbjct: 241 VEEVPIMSLFTRDNSSRGSAGTTTTTNNNNNNNNKAQKQSSDELTSEENRFSKDVIMQKY 300

Query: 301 LKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGAPPSIAAPPTSPPVEKAVTETEAVQ 360
           LKMVKPLYIRVSRRYGEKFRLSGQLILGAA AKSG  PSIAAPPTSPPVEKAVT+TE V+
Sbjct: 301 LKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGXLPSIAAPPTSPPVEKAVTDTELVE 360

Query: 361 VQAPTTTANAKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQ 420
           VQA TTT N KGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQ
Sbjct: 361 VQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQ 420

Query: 421 SAILHCKRSFNASRGKKKKLFTILLLVLKVSPFFLNFINGSFLFCFCSAYTKTSTDAESS 480
           SAILHCKRSFNASR                                         D+ESS
Sbjct: 421 SAILHCKRSFNASR-----------------------------------------DSESS 449

Query: 481 LLSRSVSDTLREKPSELTEKAI 497
           LLS      LREK S+ TEKAI
Sbjct: 481 LLS------LREKASDFTEKAI 449

BLAST of Spg029862 vs. NCBI nr
Match: KAA0044853.1 (putative membrane-associated kinase regulator 2 [Cucumis melo var. makuwa])

HSP 1 Score: 663.3 bits (1710), Expect = 1.6e-186
Identity = 391/500 (78.20%), Postives = 412/500 (82.40%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGAAAAAAAADFAFNSNSSA-GTATVVTAVAQNQADTDEEE-DGDDG 60
           MESFSLLKYWRGGG  A A AAADFAFNSNSSA  TATVVTAV + + +TDEEE D DDG
Sbjct: 1   MESFSLLKYWRGGGAGAGATAAADFAFNSNSSASATATVVTAVPRKEVETDEEENDNDDG 60

Query: 61  PFFDLEFAVPDEDEGGRKQGGNGEGRNCVDGSEEENEEDEEEEEEDGDSDG-EREFNFTL 120
           PFFDLEFAVPDEDE GRKQ     G +C+DGSEEE EED++++E+D D DG EREFNFTL
Sbjct: 61  PFFDLEFAVPDEDE-GRKQ-----GTDCIDGSEEEGEEDDDDDEDDEDGDGDEREFNFTL 120

Query: 121 SSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNTRPQLRVTLLKSAAKFRVF 180
           SSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPN RPQLRVTLLKSAAKFRVF
Sbjct: 121 SSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNVRPQLRVTLLKSAAKFRVF 180

Query: 181 MSGLKKSKLNGEKKTETDGSVGETTKSETKEEAPEKPPEKNENQPEKNQNQTKSKFFAVK 240
           MSGLKKSK NGEKKTE DGSVGET KSETKEE    P EKNENQ EK+QNQTK+KFFAVK
Sbjct: 181 MSGLKKSKSNGEKKTEMDGSVGETAKSETKEETTGNPSEKNENQLEKDQNQTKNKFFAVK 240

Query: 241 FKVEEVPMMSLFTRDNSSRGSGGITTTTNNNKPQKQSSDESTSEENRFSKDVIMQKYLKM 300
           FKVEEVP+MSLFTRDNSSRGSG     T NNKPQKQ+SDEST EE RFSKDV+MQKYLKM
Sbjct: 241 FKVEEVPIMSLFTRDNSSRGSG----VTTNNKPQKQTSDESTPEETRFSKDVLMQKYLKM 300

Query: 301 VKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGAPPSIAAPPTSPPVEKAVTETEAVQVQA 360
           VKPLYIRVSRRYGEKF+LSGQLILGAAGAKS APPSIAAPPTSP  EK+V ETEAVQV+ 
Sbjct: 301 VKPLYIRVSRRYGEKFKLSGQLILGAAGAKSSAPPSIAAPPTSPSTEKSVVETEAVQVKP 360

Query: 361 PTTTANAKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQSAI 420
           PTTT N+KGRNMPAKLRVVCKHLGKSRSASSAVAAAPPG APSRRDDSLLQQQDGIQSAI
Sbjct: 361 PTTTTNSKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGVAPSRRDDSLLQQQDGIQSAI 420

Query: 421 LHCKRSFNASRGKKKKLFTILLLVLKVSPFFLNFINGSFLFCFCSAYTKTSTDAESSLLS 480
           LHCKRSFNASRG                                         +ESSLLS
Sbjct: 421 LHCKRSFNASRG-----------------------------------------SESSLLS 449

Query: 481 RSVSDTLREKPSELTEKAIG 498
           RSVSDTLREKPS+LTEKAIG
Sbjct: 481 RSVSDTLREKPSDLTEKAIG 449

BLAST of Spg029862 vs. ExPASy Swiss-Prot
Match: Q9SH58 (Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 GN=MAKR2 PE=2 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 8.5e-53
Identity = 186/453 (41.06%), Postives = 243/453 (53.64%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGA-------AAAAAAADFAFNSNSSAGTATVVTAVAQNQADTDEEE 60
           ME+FSLL YW+  GGA       + +++++   +++  S GT T+VT+V + + D + +E
Sbjct: 1   MEAFSLLNYWKNSGGAVTFLPHSSESSSSSLCRYSAGESIGTTTIVTSVTETE-DEEADE 60

Query: 61  DGDDGPFFDLEFAVPDEDEGGRKQGGNGEGRNCVDGSEEENEEDEEEEEEDGD-SDGERE 120
           + D+GPFFDLEFA+P E+E     GG G                    E D D +DG  E
Sbjct: 61  NDDEGPFFDLEFALPAEEED--TDGGVG-------------------REGDSDCTDGGCE 120

Query: 121 FNFTLSSGSSNEHSDANLTFSPSDDLFFKGKLV-----PVEASSNGGSEPNTRPQLRVTL 180
           F FTLSS S  E     +     DD+FFK  +V     P  +     S      QL  ++
Sbjct: 121 FKFTLSSCSGGED---RVDPDSLDDVFFKETIVEEVEPPTSSEQQTCSVKAPAAQLSASI 180

Query: 181 LKSAAKFRVFMSGLKKSKLNGEKKTETDGSVGETTKSETKEEAPEKPPEKNENQPEKNQN 240
           LK+A K RVFM G+KKSKL   K     GS  E    +T       PP      PE  Q 
Sbjct: 181 LKTATKLRVFMLGMKKSKLIQAKSEANFGS--EDLDKQT------HPPPPLSLSPESQQK 240

Query: 241 QTKSKFFAVKFKVEEVPMMSLFTRDNSSRGSGGITTTTNNNKP--QKQSSDES-TSEENR 300
            T      V  KVEEVP++SLF+R+NSS+ S   +++++++    +KQ+ +ES  S+E R
Sbjct: 241 ST----VTVNLKVEEVPIISLFSRENSSKNSSSSSSSSSSSSSSLKKQNGNESVVSDEKR 300

Query: 301 FSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGAPPSIAAPPTSPPVE 360
           F   V+MQKYLK VKPLYIRVSRRYGEK R SGQL                   T+P   
Sbjct: 301 F---VMMQKYLKKVKPLYIRVSRRYGEKLRHSGQL-------------------TAPSSA 360

Query: 361 KAVTETEAVQVQAPTTTANAKGRNMPAKLRVVCKHLGKSRSAS------SAVAAAPPGQA 420
           ++  E     V   T        N+PA L+VV KHLGKSRS+S      S+  A    Q+
Sbjct: 361 RSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTATTPSSTTATVTTQS 394

Query: 421 PS-RRDDSLLQQQDGIQSAILHCKRSFNASRGK 431
            S RRDDSLLQQQD IQSAILHCKRSFN+SR K
Sbjct: 421 ESRRRDDSLLQQQDSIQSAILHCKRSFNSSRDK 394

BLAST of Spg029862 vs. ExPASy Swiss-Prot
Match: Q9FLX4 (Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 4.3e-04
Identity = 79/322 (24.53%), Postives = 131/322 (40.68%), Query Frame = 0

Query: 173 KFRVFMSGLKKSKLNGEKKTETDGSVGETTKSETKEEAPEKPPEKNENQPEKNQNQTKSK 232
           + ++ +S  K  K   +++ ET  +    + S   +  P         Q      ++  K
Sbjct: 52  ELKISLSDFKTPK--EKQRLETTTTTTTYSVSNKSKVLPFVDISSKPQQSPTTLLKSGQK 111

Query: 233 FFAVKFKVEEVPMMSLFTRDNSSRGSGGI-------TTTTNNNKPQKQSSDESTSEENRF 292
           F A  FK  E    +   ++ ++R S  +       TTTT+  K    +  + T  ++  
Sbjct: 112 FRAFSFKKSEKSTTTEKKKEENNRTSLNVRFRVDDETTTTSFRKTASIARSQQT--DDTM 171

Query: 293 SKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGAPPSIAAPPTSPPVEK 352
             D + +++  ++KPLY +                      K  +  +I +P +SP    
Sbjct: 172 FDDSVSKRFFSLIKPLYTK--------------------STKKQSSSTITSPTSSP---- 231

Query: 353 AVTETEAVQVQAPTTTANAKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDS 412
                          T   +  N+P+ +R V + LGKSRSAS+A+    P    +R D+S
Sbjct: 232 --------------ATREKQRSNIPSGMRSVRRQLGKSRSASAAIGGMSPA---NRIDES 291

Query: 413 LLQQQDGIQSAILHCKRSFNASRGKKKKLFTILLLVLKVSPFFLNFINGSFLFCFCSAYT 472
           L  QQDGIQSAILHCK+SF+ SR                     + ++ S          
Sbjct: 292 LQVQQDGIQSAILHCKKSFHGSRES-------------------SLLSRSTSESSSQEKL 309

Query: 473 KTSTDAESSLLSRSVSDTLREK 488
            TS+  +S L SR  SD++ EK
Sbjct: 352 STSSSEDSYLFSRISSDSISEK 309

BLAST of Spg029862 vs. ExPASy TrEMBL
Match: A0A6J1FPV4 (probable membrane-associated kinase regulator 2 OS=Cucurbita moschata OX=3662 GN=LOC111447389 PE=4 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 5.0e-189
Identity = 398/512 (77.73%), Postives = 421/512 (82.23%), Query Frame = 0

Query: 1   MESFSLLKYWR--GGGGAAAAAAAADFAFNSNSSAGTATVVTAVAQNQADTDEEEDGDDG 60
           MESFSLLKYWR  GGGG A+A+AAADFAF+S SSA TATVVTAVA+NQA+TDE+ED DDG
Sbjct: 1   MESFSLLKYWRGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG 60

Query: 61  PFFDLEFAVPDEDEGGRKQG----GNGEGRNCVDGSEEENEED--EEEEEEDGDSDGERE 120
           PFFDLEFAVPDEDE GRKQG    G+G+GRNC DGSEEEN++D  EEEEEEDGDSDGERE
Sbjct: 61  PFFDLEFAVPDEDE-GRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGERE 120

Query: 121 FNFTLSSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNTRPQLRVTLLKSAA 180
           F+FTLSSGS+NEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPN++PQLRVTLLKSAA
Sbjct: 121 FDFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAA 180

Query: 181 KFRVFMSGLKKSKLNGEKKTETDGSVGETTKSETKEEAPEKPPEKNENQPEKNQNQTKSK 240
           KFRVFMSGLKKSK NGEKKTE DGSVGE TK ET E  PE PPEKNENQ +K+QNQTKSK
Sbjct: 181 KFRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSK 240

Query: 241 FFAVKFKVEEVPMMSLFTRDNSSRGSGGITTTT-------NNNKPQKQSSDESTSEENRF 300
            FAVK KVEEVP+MSLFTRDNSSRGS G TTTT       N+NK QKQSSDE TSEENRF
Sbjct: 241 LFAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSDELTSEENRF 300

Query: 301 SKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGAPPSIAAPPTSPPVEK 360
           SKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAA AKSGA P+IAAPPTSPPVEK
Sbjct: 301 SKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVEK 360

Query: 361 AVTETEAVQVQAPTTTANAKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDS 420
            VT+TE V+VQA TTT N KGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDS
Sbjct: 361 TVTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDS 420

Query: 421 LLQQQDGIQSAILHCKRSFNASRGKKKKLFTILLLVLKVSPFFLNFINGSFLFCFCSAYT 480
           LLQQQDGIQSAILHCKRSFNASR                                     
Sbjct: 421 LLQQQDGIQSAILHCKRSFNASR------------------------------------- 464

Query: 481 KTSTDAESSLLSRSVSDTLREKPSELTEKAIG 498
               D+ESSLLS      LREKPS+ TEKAIG
Sbjct: 481 ----DSESSLLS------LREKPSDFTEKAIG 464

BLAST of Spg029862 vs. ExPASy TrEMBL
Match: A0A6J1J0G8 (probable membrane-associated kinase regulator 2 OS=Cucurbita maxima OX=3661 GN=LOC111480174 PE=4 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 5.5e-188
Identity = 393/502 (78.29%), Postives = 414/502 (82.47%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGAAA-AAAAADFAFNSNSSAGTATVVTAVAQNQADTDEEEDGDDGP 60
           MESFSLLKYWRGGGG  A A+AAADFAF+S SSA TATVVTAVA+NQA+TDE+ED DDGP
Sbjct: 1   MESFSLLKYWRGGGGGGAPASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDGP 60

Query: 61  FFDLEFAVPDEDEGGRKQGGNGEGRNCVDGSEEENEEDEEEEEEDGDSDGEREFNFTLSS 120
           FFDLEFAVP+EDE GRKQGG+G+GRNC DGSEEEN     ++EEDGDSDGEREF+FTLSS
Sbjct: 61  FFDLEFAVPEEDE-GRKQGGDGDGRNCDDGSEEEN-----DDEEDGDSDGEREFDFTLSS 120

Query: 121 GSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNTRPQLRVTLLKSAAKFRVFMS 180
           GS+NEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPN++PQLRVTLLKSAAKFRVFMS
Sbjct: 121 GSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAKFRVFMS 180

Query: 181 GLKKSKLNGEKKTETDGSVGETTKSETKEEAPEKPPEKNENQPEKNQNQTKSKFFAVKFK 240
           GLKKSK NGEKKTETDGSVGE TK ET E  PE PPEKNENQ +K+QNQTKSK FAVK K
Sbjct: 181 GLKKSKSNGEKKTETDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKLFAVKLK 240

Query: 241 VEEVPMMSLFTRDNSSRGSGGITTTT-----NNNKPQKQSSDESTSEENRFSKDVIMQKY 300
           VEEVP+MSLFTRDNSSRGS G TTTT     NNNK QKQSSDE TSEENRFSKDVIMQKY
Sbjct: 241 VEEVPIMSLFTRDNSSRGSAGTTTTTNNNNNNNNKAQKQSSDELTSEENRFSKDVIMQKY 300

Query: 301 LKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGAPPSIAAPPTSPPVEKAVTETEAVQ 360
           LKMVKPLYIRVSRRYGEKFRLSGQLILGAA AKSG  PSIAAPPTSPPVEKAVT+TE V+
Sbjct: 301 LKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGXLPSIAAPPTSPPVEKAVTDTELVE 360

Query: 361 VQAPTTTANAKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQ 420
           VQA TTT N KGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQ
Sbjct: 361 VQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQ 420

Query: 421 SAILHCKRSFNASRGKKKKLFTILLLVLKVSPFFLNFINGSFLFCFCSAYTKTSTDAESS 480
           SAILHCKRSFNASR                                         D+ESS
Sbjct: 421 SAILHCKRSFNASR-----------------------------------------DSESS 449

Query: 481 LLSRSVSDTLREKPSELTEKAI 497
           LLS      LREK S+ TEKAI
Sbjct: 481 LLS------LREKASDFTEKAI 449

BLAST of Spg029862 vs. ExPASy TrEMBL
Match: A0A5A7TMX4 (Putative membrane-associated kinase regulator 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G001450 PE=4 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 7.9e-187
Identity = 391/500 (78.20%), Postives = 412/500 (82.40%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGAAAAAAAADFAFNSNSSA-GTATVVTAVAQNQADTDEEE-DGDDG 60
           MESFSLLKYWRGGG  A A AAADFAFNSNSSA  TATVVTAV + + +TDEEE D DDG
Sbjct: 1   MESFSLLKYWRGGGAGAGATAAADFAFNSNSSASATATVVTAVPRKEVETDEEENDNDDG 60

Query: 61  PFFDLEFAVPDEDEGGRKQGGNGEGRNCVDGSEEENEEDEEEEEEDGDSDG-EREFNFTL 120
           PFFDLEFAVPDEDE GRKQ     G +C+DGSEEE EED++++E+D D DG EREFNFTL
Sbjct: 61  PFFDLEFAVPDEDE-GRKQ-----GTDCIDGSEEEGEEDDDDDEDDEDGDGDEREFNFTL 120

Query: 121 SSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNTRPQLRVTLLKSAAKFRVF 180
           SSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPN RPQLRVTLLKSAAKFRVF
Sbjct: 121 SSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNVRPQLRVTLLKSAAKFRVF 180

Query: 181 MSGLKKSKLNGEKKTETDGSVGETTKSETKEEAPEKPPEKNENQPEKNQNQTKSKFFAVK 240
           MSGLKKSK NGEKKTE DGSVGET KSETKEE    P EKNENQ EK+QNQTK+KFFAVK
Sbjct: 181 MSGLKKSKSNGEKKTEMDGSVGETAKSETKEETTGNPSEKNENQLEKDQNQTKNKFFAVK 240

Query: 241 FKVEEVPMMSLFTRDNSSRGSGGITTTTNNNKPQKQSSDESTSEENRFSKDVIMQKYLKM 300
           FKVEEVP+MSLFTRDNSSRGSG     T NNKPQKQ+SDEST EE RFSKDV+MQKYLKM
Sbjct: 241 FKVEEVPIMSLFTRDNSSRGSG----VTTNNKPQKQTSDESTPEETRFSKDVLMQKYLKM 300

Query: 301 VKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGAPPSIAAPPTSPPVEKAVTETEAVQVQA 360
           VKPLYIRVSRRYGEKF+LSGQLILGAAGAKS APPSIAAPPTSP  EK+V ETEAVQV+ 
Sbjct: 301 VKPLYIRVSRRYGEKFKLSGQLILGAAGAKSSAPPSIAAPPTSPSTEKSVVETEAVQVKP 360

Query: 361 PTTTANAKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQSAI 420
           PTTT N+KGRNMPAKLRVVCKHLGKSRSASSAVAAAPPG APSRRDDSLLQQQDGIQSAI
Sbjct: 361 PTTTTNSKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGVAPSRRDDSLLQQQDGIQSAI 420

Query: 421 LHCKRSFNASRGKKKKLFTILLLVLKVSPFFLNFINGSFLFCFCSAYTKTSTDAESSLLS 480
           LHCKRSFNASRG                                         +ESSLLS
Sbjct: 421 LHCKRSFNASRG-----------------------------------------SESSLLS 449

Query: 481 RSVSDTLREKPSELTEKAIG 498
           RSVSDTLREKPS+LTEKAIG
Sbjct: 481 RSVSDTLREKPSDLTEKAIG 449

BLAST of Spg029862 vs. ExPASy TrEMBL
Match: A0A5D3CXJ8 (Putative membrane-associated kinase regulator 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004410 PE=4 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 3.9e-186
Identity = 392/500 (78.40%), Postives = 412/500 (82.40%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGAAAAAAAADFAFNSNSSA-GTATVVTAVAQNQADTDEEE-DGDDG 60
           MESFSLLKYWRGGG  A A AAADFAFNSNSSA  TATVVTAV + + +TDEEE D DDG
Sbjct: 1   MESFSLLKYWRGGGAGAGATAAADFAFNSNSSASATATVVTAVPRKEVETDEEENDNDDG 60

Query: 61  PFFDLEFAVPDEDEGGRKQGGNGEGRNCVDGSEEENEE-DEEEEEEDGDSDGEREFNFTL 120
           PFFDLEFAVPDEDE GRKQ     G +C+DGSEEE EE D++E++EDGD D EREFNFTL
Sbjct: 61  PFFDLEFAVPDEDE-GRKQ-----GTDCIDGSEEEGEEDDDDEDDEDGDGD-EREFNFTL 120

Query: 121 SSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNTRPQLRVTLLKSAAKFRVF 180
           SSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPN RPQLRVTLLKSAAKFRVF
Sbjct: 121 SSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNVRPQLRVTLLKSAAKFRVF 180

Query: 181 MSGLKKSKLNGEKKTETDGSVGETTKSETKEEAPEKPPEKNENQPEKNQNQTKSKFFAVK 240
           MSGLKKSK NGEKKTE DGSVGET KSET EE  E P EKNENQ EK+QNQTK+KFFAVK
Sbjct: 181 MSGLKKSKSNGEKKTEMDGSVGETAKSETNEETTENPSEKNENQLEKDQNQTKNKFFAVK 240

Query: 241 FKVEEVPMMSLFTRDNSSRGSGGITTTTNNNKPQKQSSDESTSEENRFSKDVIMQKYLKM 300
           FKVEEVP+MSLFTRDNSSRGSG     T NNKPQKQ+SDEST EE RFSKDV+MQKYLKM
Sbjct: 241 FKVEEVPIMSLFTRDNSSRGSG----VTTNNKPQKQTSDESTPEEARFSKDVLMQKYLKM 300

Query: 301 VKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGAPPSIAAPPTSPPVEKAVTETEAVQVQA 360
           VKPLYIRVSRRYGEKF+LSGQLILGAAGAKS APPSIAAPPTSP  EK+V ETEAVQV+ 
Sbjct: 301 VKPLYIRVSRRYGEKFKLSGQLILGAAGAKSSAPPSIAAPPTSPSTEKSVVETEAVQVKP 360

Query: 361 PTTTANAKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQSAI 420
           PTTT N+KGRNMPAKLRVVCKHLGKSRSASSAVAAAPPG APSRRDDSLLQQQDGIQSAI
Sbjct: 361 PTTTTNSKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGVAPSRRDDSLLQQQDGIQSAI 420

Query: 421 LHCKRSFNASRGKKKKLFTILLLVLKVSPFFLNFINGSFLFCFCSAYTKTSTDAESSLLS 480
           LHCKRSFNASRG                                         +ESSLLS
Sbjct: 421 LHCKRSFNASRG-----------------------------------------SESSLLS 448

Query: 481 RSVSDTLREKPSELTEKAIG 498
           RSVSDTLREKPS+LTEKAIG
Sbjct: 481 RSVSDTLREKPSDLTEKAIG 448

BLAST of Spg029862 vs. ExPASy TrEMBL
Match: A0A1S3BTL4 (probable membrane-associated kinase regulator 2 OS=Cucumis melo OX=3656 GN=LOC103493135 PE=4 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 3.9e-186
Identity = 392/500 (78.40%), Postives = 412/500 (82.40%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGAAAAAAAADFAFNSNSSA-GTATVVTAVAQNQADTDEEE-DGDDG 60
           MESFSLLKYWRGGG  A A AAADFAFNSNSSA  TATVVTAV + + +TDEEE D DDG
Sbjct: 1   MESFSLLKYWRGGGAGAGATAAADFAFNSNSSASATATVVTAVPRKEVETDEEENDNDDG 60

Query: 61  PFFDLEFAVPDEDEGGRKQGGNGEGRNCVDGSEEENEE-DEEEEEEDGDSDGEREFNFTL 120
           PFFDLEFAVPDEDE GRKQ     G +C+DGSEEE EE D++E++EDGD D EREFNFTL
Sbjct: 61  PFFDLEFAVPDEDE-GRKQ-----GTDCIDGSEEEGEEDDDDEDDEDGDGD-EREFNFTL 120

Query: 121 SSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNTRPQLRVTLLKSAAKFRVF 180
           SSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPN RPQLRVTLLKSAAKFRVF
Sbjct: 121 SSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNVRPQLRVTLLKSAAKFRVF 180

Query: 181 MSGLKKSKLNGEKKTETDGSVGETTKSETKEEAPEKPPEKNENQPEKNQNQTKSKFFAVK 240
           MSGLKKSK NGEKKTE DGSVGET KSET EE  E P EKNENQ EK+QNQTK+KFFAVK
Sbjct: 181 MSGLKKSKSNGEKKTEMDGSVGETAKSETNEETTENPSEKNENQLEKDQNQTKNKFFAVK 240

Query: 241 FKVEEVPMMSLFTRDNSSRGSGGITTTTNNNKPQKQSSDESTSEENRFSKDVIMQKYLKM 300
           FKVEEVP+MSLFTRDNSSRGSG     T NNKPQKQ+SDEST EE RFSKDV+MQKYLKM
Sbjct: 241 FKVEEVPIMSLFTRDNSSRGSG----VTTNNKPQKQTSDESTPEEARFSKDVLMQKYLKM 300

Query: 301 VKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGAPPSIAAPPTSPPVEKAVTETEAVQVQA 360
           VKPLYIRVSRRYGEKF+LSGQLILGAAGAKS APPSIAAPPTSP  EK+V ETEAVQV+ 
Sbjct: 301 VKPLYIRVSRRYGEKFKLSGQLILGAAGAKSSAPPSIAAPPTSPSTEKSVVETEAVQVKP 360

Query: 361 PTTTANAKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQSAI 420
           PTTT N+KGRNMPAKLRVVCKHLGKSRSASSAVAAAPPG APSRRDDSLLQQQDGIQSAI
Sbjct: 361 PTTTTNSKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGVAPSRRDDSLLQQQDGIQSAI 420

Query: 421 LHCKRSFNASRGKKKKLFTILLLVLKVSPFFLNFINGSFLFCFCSAYTKTSTDAESSLLS 480
           LHCKRSFNASRG                                         +ESSLLS
Sbjct: 421 LHCKRSFNASRG-----------------------------------------SESSLLS 448

Query: 481 RSVSDTLREKPSELTEKAIG 498
           RSVSDTLREKPS+LTEKAIG
Sbjct: 481 RSVSDTLREKPSDLTEKAIG 448

BLAST of Spg029862 vs. TAIR 10
Match: AT1G64080.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G52870.1); Has 1546 Blast hits to 738 proteins in 176 species: Archae - 0; Bacteria - 143; Metazoa - 278; Fungi - 131; Plants - 95; Viruses - 4; Other Eukaryotes - 895 (source: NCBI BLink). )

HSP 1 Score: 209.5 bits (532), Expect = 6.0e-54
Identity = 186/453 (41.06%), Postives = 243/453 (53.64%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGA-------AAAAAAADFAFNSNSSAGTATVVTAVAQNQADTDEEE 60
           ME+FSLL YW+  GGA       + +++++   +++  S GT T+VT+V + + D + +E
Sbjct: 1   MEAFSLLNYWKNSGGAVTFLPHSSESSSSSLCRYSAGESIGTTTIVTSVTETE-DEEADE 60

Query: 61  DGDDGPFFDLEFAVPDEDEGGRKQGGNGEGRNCVDGSEEENEEDEEEEEEDGD-SDGERE 120
           + D+GPFFDLEFA+P E+E     GG G                    E D D +DG  E
Sbjct: 61  NDDEGPFFDLEFALPAEEED--TDGGVG-------------------REGDSDCTDGGCE 120

Query: 121 FNFTLSSGSSNEHSDANLTFSPSDDLFFKGKLV-----PVEASSNGGSEPNTRPQLRVTL 180
           F FTLSS S  E     +     DD+FFK  +V     P  +     S      QL  ++
Sbjct: 121 FKFTLSSCSGGED---RVDPDSLDDVFFKETIVEEVEPPTSSEQQTCSVKAPAAQLSASI 180

Query: 181 LKSAAKFRVFMSGLKKSKLNGEKKTETDGSVGETTKSETKEEAPEKPPEKNENQPEKNQN 240
           LK+A K RVFM G+KKSKL   K     GS  E    +T       PP      PE  Q 
Sbjct: 181 LKTATKLRVFMLGMKKSKLIQAKSEANFGS--EDLDKQT------HPPPPLSLSPESQQK 240

Query: 241 QTKSKFFAVKFKVEEVPMMSLFTRDNSSRGSGGITTTTNNNKP--QKQSSDES-TSEENR 300
            T      V  KVEEVP++SLF+R+NSS+ S   +++++++    +KQ+ +ES  S+E R
Sbjct: 241 ST----VTVNLKVEEVPIISLFSRENSSKNSSSSSSSSSSSSSSLKKQNGNESVVSDEKR 300

Query: 301 FSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGAPPSIAAPPTSPPVE 360
           F   V+MQKYLK VKPLYIRVSRRYGEK R SGQL                   T+P   
Sbjct: 301 F---VMMQKYLKKVKPLYIRVSRRYGEKLRHSGQL-------------------TAPSSA 360

Query: 361 KAVTETEAVQVQAPTTTANAKGRNMPAKLRVVCKHLGKSRSAS------SAVAAAPPGQA 420
           ++  E     V   T        N+PA L+VV KHLGKSRS+S      S+  A    Q+
Sbjct: 361 RSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTATTPSSTTATVTTQS 394

Query: 421 PS-RRDDSLLQQQDGIQSAILHCKRSFNASRGK 431
            S RRDDSLLQQQD IQSAILHCKRSFN+SR K
Sbjct: 421 ESRRRDDSLLQQQDSIQSAILHCKRSFNSSRDK 394

BLAST of Spg029862 vs. TAIR 10
Match: AT5G52870.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64080.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 47.8 bits (112), Expect = 3.0e-05
Identity = 79/322 (24.53%), Postives = 131/322 (40.68%), Query Frame = 0

Query: 173 KFRVFMSGLKKSKLNGEKKTETDGSVGETTKSETKEEAPEKPPEKNENQPEKNQNQTKSK 232
           + ++ +S  K  K   +++ ET  +    + S   +  P         Q      ++  K
Sbjct: 52  ELKISLSDFKTPK--EKQRLETTTTTTTYSVSNKSKVLPFVDISSKPQQSPTTLLKSGQK 111

Query: 233 FFAVKFKVEEVPMMSLFTRDNSSRGSGGI-------TTTTNNNKPQKQSSDESTSEENRF 292
           F A  FK  E    +   ++ ++R S  +       TTTT+  K    +  + T  ++  
Sbjct: 112 FRAFSFKKSEKSTTTEKKKEENNRTSLNVRFRVDDETTTTSFRKTASIARSQQT--DDTM 171

Query: 293 SKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSGAPPSIAAPPTSPPVEK 352
             D + +++  ++KPLY +                      K  +  +I +P +SP    
Sbjct: 172 FDDSVSKRFFSLIKPLYTK--------------------STKKQSSSTITSPTSSP---- 231

Query: 353 AVTETEAVQVQAPTTTANAKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDS 412
                          T   +  N+P+ +R V + LGKSRSAS+A+    P    +R D+S
Sbjct: 232 --------------ATREKQRSNIPSGMRSVRRQLGKSRSASAAIGGMSPA---NRIDES 291

Query: 413 LLQQQDGIQSAILHCKRSFNASRGKKKKLFTILLLVLKVSPFFLNFINGSFLFCFCSAYT 472
           L  QQDGIQSAILHCK+SF+ SR                     + ++ S          
Sbjct: 292 LQVQQDGIQSAILHCKKSFHGSRES-------------------SLLSRSTSESSSQEKL 309

Query: 473 KTSTDAESSLLSRSVSDTLREK 488
            TS+  +S L SR  SD++ EK
Sbjct: 352 STSSSEDSYLFSRISSDSISEK 309

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6600544.12.9e-19178.43putative membrane-associated kinase regulator 2, partial [Cucurbita argyrosperma... [more]
XP_023529752.11.4e-19079.13probable membrane-associated kinase regulator 2 [Cucurbita pepo subsp. pepo][more]
XP_022942302.11.0e-18877.73probable membrane-associated kinase regulator 2 [Cucurbita moschata][more]
XP_022980889.11.1e-18778.29probable membrane-associated kinase regulator 2 [Cucurbita maxima][more]
KAA0044853.11.6e-18678.20putative membrane-associated kinase regulator 2 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9SH588.5e-5341.06Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9FLX44.3e-0424.53Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FPV45.0e-18977.73probable membrane-associated kinase regulator 2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1J0G85.5e-18878.29probable membrane-associated kinase regulator 2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A5A7TMX47.9e-18778.20Putative membrane-associated kinase regulator 2 OS=Cucumis melo var. makuwa OX=1... [more]
A0A5D3CXJ83.9e-18678.40Putative membrane-associated kinase regulator 2 OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3BTL43.9e-18678.40probable membrane-associated kinase regulator 2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
AT1G64080.16.0e-5441.06unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT5G52870.13.0e-0524.53unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..109
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 179..230
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 249..274
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 249..284
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 47..67
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..137
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 112..133
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 184..220
NoneNo IPR availablePANTHERPTHR33929:SF1MEMBRANE-ASSOCIATED KINASE REGULATOR 2-RELATEDcoord: 1..492
IPR039619Membrane-associated kinase regulator 2/5PANTHERPTHR33929MEMBRANE-ASSOCIATED KINASE REGULATOR 2-RELATEDcoord: 1..492

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg029862.1Spg029862.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
cellular_component GO:0005886 plasma membrane
molecular_function GO:0016301 kinase activity