Spg029776 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg029776 vs. NCBI nr
Match: XP_004146562.1 (subtilisin-like protease SBT3.6 [Cucumis sativus] >KGN53334.1 hypothetical protein Csa_014762 [Cucumis sativus])

HSP 1 Score: 1087.8 bits (2812), Expect = 0.0e+00
Identity = 547/762 (71.78%), Postives = 632/762 (82.94%), Query Frame = 0

Query: 6   GRLIVGVMTIYALFSMFAYKP-MAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDLLATV 65
           G LIVG +    LF     KP +AEADDQN KVHIVYLGE+ H DTK T  SHH LL+T+
Sbjct: 8   GHLIVGFIIFDCLF-----KPILAEADDQNPKVHIVYLGEKPHHDTKFTIDSHHQLLSTI 67

Query: 66  LGSKEKSSESMVYSYRHGFSGFAAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRSWDFL 125
           LGSKEKS E+MVYSY+HGFSGFAAKLT+SQAQKL+E+  V+RV PSSLYK+HTTRSWDFL
Sbjct: 68  LGSKEKSMEAMVYSYKHGFSGFAAKLTKSQAQKLSEMSRVVRVVPSSLYKVHTTRSWDFL 127

Query: 126 GLSSSPSESSNLLHRAKMGDNVIIGVVDSGYWPESESFGDKGLGPVPSRWKGTCESGEEF 185
           GLSSSP ESSNLLHRA+MG+NVIIGV+D+G WPESESF DKG+G +PSRWKGTCESGE+F
Sbjct: 128 GLSSSPFESSNLLHRAQMGENVIIGVIDTGIWPESESFKDKGVGSIPSRWKGTCESGEQF 187

Query: 186 NSTNCNRKVIGARWYMKGFVADNGREALADEYLSPRDINGHGTHTASTAAGSFVTNVSYH 245
           NSTNCN+K+IGARW+MKGFVAD GR+ALA EYLSPRD+NGHGTHTAS AAGSFV N++YH
Sbjct: 188 NSTNCNKKIIGARWFMKGFVADLGRDALAKEYLSPRDLNGHGTHTASIAAGSFVANINYH 247

Query: 246 GLGVGTLRGGAPLARLAIYKALWTSDCVGSAADILKAIDEAIHDGVDVLSISIGRFIPLF 305
               GT+RGGAPLARLAIYKALWT D VGS ADILKAIDEAI+DGVDVLS+SIG   P  
Sbjct: 248 NNAAGTVRGGAPLARLAIYKALWTKDAVGSTADILKAIDEAINDGVDVLSMSIGSLTPFL 307

Query: 306 PEFNEENEIAIGSFHAVARGISVVCAGGNDGPLQQTVQNTAPWILTVAASTMDRAFLSSI 365
           PEFNE N+IA GSFHA+A+GISVVCA GN GP  QTV+N APWI TVAA+T+DRAFL+SI
Sbjct: 308 PEFNEANDIAFGSFHAIAKGISVVCAAGNSGPTPQTVENVAPWIFTVAANTIDRAFLASI 367

Query: 366 -TLPDNTTYLGQSFYDSEKELVAKLTTASNGRCSDILGNETFISGKVVLCFSDIADKTAA 425
            TLPDNTT+LGQS  DS+K+LVA+L T   GRC D+LGNETFI+GKVV+CFS++AD    
Sbjct: 368 TTLPDNTTFLGQSLLDSKKDLVAELETLDTGRCDDLLGNETFINGKVVMCFSNLADHNTI 427

Query: 426 NMAAAAAVKANGSGIIVAGQQDDDLFAC-KQPFPCILVDNTVGTKLLF-HVLEGS-NPMV 485
             AA A  +ANG+GIIVAGQQDDDLF+C   P PCILVD  VG+KL F ++L+ S NP+V
Sbjct: 428 YDAAMAVARANGTGIIVAGQQDDDLFSCIPSPIPCILVDTDVGSKLFFINLLQNSTNPVV 487

Query: 486 RLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKRDIAAPGSNILAAISPHHSSNDKGFG 545
           RL   +TIIGKPI+  I YFSSRGPNS S  ILK DI+APGSNILAA+SPHH  N+KGF 
Sbjct: 488 RLRATRTIIGKPITPAISYFSSRGPNSVSNPILKPDISAPGSNILAAVSPHHIFNEKGFM 547

Query: 546 MMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITTARAKSPSGLPIFANGSPPKAAD 605
           ++SGTSMA PHIS IVALL+S+ PTWSPAAIKSAL+TTAR +   GLPIFA G+PPK AD
Sbjct: 548 LLSGTSMATPHISAIVALLKSVHPTWSPAAIKSALMTTARTEVSPGLPIFAEGTPPKMAD 607

Query: 606 PFDHGGGVVDPNAAIDPGLIYDLDTIEYI-YYLCGMGYKGSDISQLTQQKTECPFPRPSV 665
           PFD+GGG+VD NAA+DPGL+YD+   +YI YYLCGMGYK  DIS LTQ+KT CP  R SV
Sbjct: 608 PFDYGGGIVDANAAVDPGLVYDMGRKDYIDYYLCGMGYKDEDISHLTQRKTVCPLQRLSV 667

Query: 666 LDLNLPTITVPELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVLAFNSKVRK 725
           LDLNLP IT+P L NST VTRTVTNVGN++ +Y+A IE+P G KV+V PQVL FNS+V+K
Sbjct: 668 LDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAEIESPFGCKVSVNPQVLVFNSQVKK 727

Query: 726 ISFKVTFSTALQNNYGYSFGSLTWTDGVHLVKTPLTVRIDFF 762
           ISFKV F T +Q NYGYSFG LTWTDG+H+VK PL+VR  FF
Sbjct: 728 ISFKVMFFTQVQRNYGYSFGRLTWTDGIHVVKIPLSVRFGFF 764

BLAST of Spg029776 vs. NCBI nr
Match: XP_022984814.1 (subtilisin-like protease SBT3.9 [Cucurbita maxima])

HSP 1 Score: 1047.7 bits (2708), Expect = 4.7e-302
Identity = 533/762 (69.95%), Postives = 616/762 (80.84%), Query Frame = 0

Query: 3   NSQGRLIVGVMTIYALFSMFAYKPMAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDLLA 62
           N     I+ V+TIYA+FS      MAEADDQN KVHIVYLGER +DD KLTT SHH+LL 
Sbjct: 4   NRAPSFILAVVTIYAVFS-----DMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHELLE 63

Query: 63  TVLGSKEKSSESMVYSYRHGFSGFAAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRSWD 122
           +VLGSKEKS ESMVYSYRHGFSGFAAKLT SQAQKLA +P V RVFPSSLYK+ TTRSWD
Sbjct: 64  SVLGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTRSWD 123

Query: 123 FLGLSSSPSESSNLLHRAKMGDNVIIGVVDSGYWPESESFGDKGLGPVPSRWKGTCESGE 182
           FLGLSSSPSESSNL HRAKMGDNVIIGV+D+G+WPESESF DKG+GP+PSRWKG C+SGE
Sbjct: 124 FLGLSSSPSESSNLFHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGE 183

Query: 183 EFNSTNCNRKVIGARWYMKGFVADNGREALADEYLSPRDINGHGTHTASTAAGSFVTNVS 242
           +FNS++CN+KVIGARW+    +AD+G EA+  +YLS RD  GHGTHTASTA G+FV NVS
Sbjct: 184 DFNSSHCNKKVIGARWFASALIADHGEEAVFKDYLSARDNEGHGTHTASTAGGAFVRNVS 243

Query: 243 YHGLGVGTLRGGAPLARLAIYKALWTSDCVGSAADILKAIDEAIHDGVDVLSISIGRFIP 302
           Y G G GTLRGGAPLARLAIYK LW+   +GS ADILK IDEAIHDGVDVLS+SIG+ IP
Sbjct: 244 YFGNGRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIGKSIP 303

Query: 303 LFPEFNEENEIAIGSFHAVARGISVVCAGGNDGPLQQTVQNTAPWILTVAASTMDRAFLS 362
           LFP+ N+ N +A+GSFHA+A+GISVVCAGGN+G +QQTV+N APW+ TVAAST+DRAFL 
Sbjct: 304 LFPDVNDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLV 363

Query: 363 SI-TLPDNTTYLGQSFYDSEKELVAKLTTASNGRCSDILGNETFISGKVV-LCFSDIADK 422
           SI TL DN TYLGQ F    K++V  L  A +GRC+ ILG +  ISG VV LCF+D+A  
Sbjct: 364 SITTLGDNATYLGQMFL--RKDIVGML-VAMDGRCAGILGGDIPISGNVVLLCFTDLAKT 423

Query: 423 TAANMAAAAAVKANGSGIIVAGQQDDDLFACKQPFPCILVDNTVGTKLLFHVLEGSNPMV 482
             A+ A     +A   G+I AGQQ D+L  C    PCI VD  VGTKL  ++L   + ++
Sbjct: 424 APASNAVMPGKQAKVVGVIYAGQQTDNLVPC--DIPCIHVDTHVGTKLFTYLLNDDDALI 483

Query: 483 RLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKRDIAAPGSNILAAISPHHSSNDKGFG 542
           RL   +TIIGKPIS+RI YFSSRGPNS SP ILK DIAAPG+NI+AA+ P+H   DKGF 
Sbjct: 484 RLRATRTIIGKPISSRIAYFSSRGPNSFSPGILKPDIAAPGANIIAAVPPNHKGGDKGFA 543

Query: 543 MMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITTARAKSPSGLPIFANGSPPKAAD 602
            MSGTSMA PHISGIV L++SL PTWSPAAIKSALITTAR + PSG+PIFA GSPPK AD
Sbjct: 544 TMSGTSMATPHISGIVTLIKSLHPTWSPAAIKSALITTARVEDPSGMPIFAEGSPPKVAD 603

Query: 603 PFDHGGGVVDPNAAIDPGLIYDLDTIEYI-YYLCGMGYKGSDISQLTQQKTECPFPRPSV 662
           PFD+GGGVVD NAA+DPGLIYDL   +YI YYLC MGY  +DIS L+QQKT CP  R S+
Sbjct: 604 PFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASI 663

Query: 663 LDLNLPTITVPELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVLAFNSKVRK 722
           LDLNLPTITVP L NSTTVTRTVTNVGN+T++Y+A+I+APPGSKV V+P+VL FNS V+K
Sbjct: 664 LDLNLPTITVPALINSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKK 723

Query: 723 ISFKVTFSTALQNNYGYSFGSLTWTDGVHLVKTPLTVRIDFF 762
           ISFKV FS+ LQ NYGYSFGSLTWTDGVHLVK+PL+VR DFF
Sbjct: 724 ISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDFF 755

BLAST of Spg029776 vs. NCBI nr
Match: XP_008452040.1 (PREDICTED: subtilisin-like protease SBT3.7 isoform X1 [Cucumis melo])

HSP 1 Score: 1043.1 bits (2696), Expect = 1.2e-300
Identity = 533/766 (69.58%), Postives = 624/766 (81.46%), Query Frame = 0

Query: 2   VNSQ-GRLIVGVMTIYALFSMFAYKPMAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDL 61
           +NSQ   LIVG +  Y L        +AE++DQN KVHIVYLGE+ H DTK TT SHH L
Sbjct: 3   INSQLAHLIVGFIIFYGLCEPI----LAESNDQNPKVHIVYLGEKPHHDTKFTTDSHHQL 62

Query: 62  LATVLGSKEKSSESMVYSYRHGFSGFAAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRS 121
           LA +LGSKEKS E+MVYSY+HGFSGFAAKLT+S+AQKL+E+  V+RV PSSLYK+HTTRS
Sbjct: 63  LAAILGSKEKSLEAMVYSYKHGFSGFAAKLTKSEAQKLSEMSRVVRVVPSSLYKVHTTRS 122

Query: 122 WDFLGLSSSPSESSNLLHRAKMGDNVIIGVVDSGYWPESESFGDKGLGPVPSRWKGTCES 181
           WDFLGLSSSPSESSNLLHRAK GD+VIIGV+DSG WPESESF DKGLGP+PSRWKGTCES
Sbjct: 123 WDFLGLSSSPSESSNLLHRAK-GDDVIIGVIDSGIWPESESFKDKGLGPIPSRWKGTCES 182

Query: 182 GEEFNSTNCNRKVIGARWYMKGFVADNGREALADEYLSPRDINGHGTHTASTAAGSFVTN 241
           GE+FNSTNCN+K+IGARW++K FVAD GREALA EYLSPRD++GHGTHTASTAAGSFV N
Sbjct: 183 GEQFNSTNCNKKIIGARWFVKAFVADYGREALAKEYLSPRDLHGHGTHTASTAAGSFVAN 242

Query: 242 VSYHGLGVGTLRGGAPLARLAIYKALWTSDCVGSAADILKAIDEAIHDGVDVLSISIGRF 301
           ++YH    GT RGGAPLARLAIYKALWT+  VGS+ADILKAIDEAIHDGVDVLSISIG  
Sbjct: 243 INYHNNAAGTARGGAPLARLAIYKALWTNRGVGSSADILKAIDEAIHDGVDVLSISIGGS 302

Query: 302 IPLFPEFNEENEIAIGSFHAVARGISVVCAGGNDGPLQQTVQNTAPWILTVAASTMDRAF 361
            P +PEF E ++IA GSFHA+ +GISVVCA GN GP  Q V N APWI TVA +T+DRAF
Sbjct: 303 PPFYPEFTELSDIAFGSFHAITKGISVVCAAGNSGPSPQMVDNVAPWIFTVATNTIDRAF 362

Query: 362 LSSI-TLPDNTTYLGQSFYDSEKELVAKLTTASNGRCSDILGNETFISGKVVLCFSDIAD 421
           LSSI TL DNTT++GQS  +S+K+LVA+L   S  RC  + GNE FI+GKVVLCF  +AD
Sbjct: 363 LSSITTLRDNTTFMGQSLLESKKDLVAEL--VSWERCDQLSGNEAFINGKVVLCFPKLAD 422

Query: 422 KTAANMAAAAAVKANGSGIIVAGQQ-DDDLFAC-KQPFPCILVDNTVGTKLLFHVLEGS- 481
            +  + AA   V+ANG+GIIVAGQQ D++L AC   P PCILVD  VG+KL F+ L+ S 
Sbjct: 423 YSTISKAAEVVVRANGTGIIVAGQQVDNNLLACISSPIPCILVDTIVGSKLFFYFLQNSD 482

Query: 482 NPMVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKRDIAAPGSNILAAISPHHSSND 541
           +P+V L  A+TIIGKPI+  I +FSSRGPNS SP ILK DI+APGSNIL+A+SPH+  N+
Sbjct: 483 HPVVMLRAARTIIGKPIAPTIAHFSSRGPNSVSPPILKPDISAPGSNILSAVSPHYFFNE 542

Query: 542 KGFGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITTARAKSPSGLPIFANGSPP 601
           KGF +MSGTSMA PH+S IVALL+S+ PTWSPAAIKSAL+TTAR +   GLPIFA+G+PP
Sbjct: 543 KGFSLMSGTSMATPHVSAIVALLKSVHPTWSPAAIKSALMTTARTEVSPGLPIFADGTPP 602

Query: 602 KAADPFDHGGGVVDPNAAIDPGLIYDLDTIEYI-YYLCGMGYKGSDISQLTQQKTECPFP 661
           K ADPFD+G GVVD NAA+DPGLIYD+   +YI YYLCGMGY+  DIS LT +KTECP  
Sbjct: 603 KVADPFDYGAGVVDANAAVDPGLIYDMGRKDYIDYYLCGMGYEDKDISHLTHRKTECPLQ 662

Query: 662 RPSVLDLNLPTITVPELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVLAFNS 721
           R S+LDLNLP IT+P L NST VTRTVTNVGN++ +Y+A IEAP G KV+V PQVL FNS
Sbjct: 663 RLSLLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAKIEAPFGCKVSVNPQVLVFNS 722

Query: 722 KVRKISFKVTFSTALQNNYGYSFGSLTWTDGVHLVKTPLTVRIDFF 762
           +V+KISFKV F T +Q NYGYSFG LTW+DGVH+VK PL+VR  FF
Sbjct: 723 QVKKISFKVMFFTQVQRNYGYSFGRLTWSDGVHVVKIPLSVRFGFF 761

BLAST of Spg029776 vs. NCBI nr
Match: XP_023552781.1 (subtilisin-like protease SBT3.7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1043.1 bits (2696), Expect = 1.2e-300
Identity = 535/757 (70.67%), Postives = 616/757 (81.37%), Query Frame = 0

Query: 8   LIVGVMTIYALFSMFAYKPMAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDLLATVLGS 67
           L+V V+TIY +FS      MAEADDQN KVHIVYLGER +DD KLTT SHH+LL +VLGS
Sbjct: 11  LVVAVVTIYGVFS-----AMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHELLESVLGS 70

Query: 68  KEKSSESMVYSYRHGFSGFAAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRSWDFLGLS 127
           KEKS ES+VYSYRHGFSGFAAKLT SQAQKLA +P V RVFPS LYK+ TTRSWDFLGLS
Sbjct: 71  KEKSLESIVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSPLYKMRTTRSWDFLGLS 130

Query: 128 SSPSESSNLLHRAKMGDNVIIGVVDSGYWPESESFGDKGLGPVPSRWKGTCESGEEFNST 187
           SSPS SSNLLHRAKMGDNVIIGV+D+G+WPESESF DKG+GP+PSRWKG CESGE+FNS+
Sbjct: 131 SSPSASSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICESGEDFNSS 190

Query: 188 NCNRKVIGARWYMKGFVADNGREALADEYLSPRDINGHGTHTASTAAGSFVTNVSYHGLG 247
           +CN+KVIGARW+    VAD+G EA+  +YLS RD  GHGTHTASTA G+FV NVS  G G
Sbjct: 191 HCNKKVIGARWFASALVADHGEEAVFMDYLSARDNQGHGTHTASTAGGAFVRNVSLFGNG 250

Query: 248 VGTLRGGAPLARLAIYKALWTSDCVGSAADILKAIDEAIHDGVDVLSISIGRFIPLFPEF 307
            GTLRGGAPLARLAIYK LW+   +GS ADILK IDEAIHDGVDVLS+SIG+ IPLF + 
Sbjct: 251 RGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIGKSIPLFSDV 310

Query: 308 NEENEIAIGSFHAVARGISVVCAGGNDGPLQQTVQNTAPWILTVAASTMDRAFLSSI-TL 367
           NE N +A+GSFHA+A+GISVVCAGGN+G +QQTV+N APW+ TVAAST+DRAFL+SI TL
Sbjct: 311 NELNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLASITTL 370

Query: 368 PDNTTYLGQSFYDSEKELVAKLTTASNGRCSDILGNETFISGKVV-LCFSDIADKTAANM 427
            DN TYLGQ+F   +K++V  L  A +GRC+ +LG+   ISG VV LCF+D+A K  A+ 
Sbjct: 371 GDNATYLGQTFL--KKDIVGML-LAMDGRCAGLLGSNIHISGNVVLLCFTDLAKKAGASN 430

Query: 428 AAAAAVKANGSGIIVAGQQDDDLFACKQPFPCILVDNTVGTKLLFHVLEGSNPMVRLGRA 487
                 +A   GII AGQ +D L  C    PCI VD  VGTKL  + L      +RL   
Sbjct: 431 VVMPGKQAKVVGIIYAGQHNDILGPCDT--PCIHVDTHVGTKLFTYYLNDEKAFIRLRAT 490

Query: 488 KTIIGKPISTRIVYFSSRGPNSASPAILKRDIAAPGSNILAAISPHHSSNDKGFGMMSGT 547
           +TI GKPIS+RI YFSSRGPNS SP ILK DIAAPGSNI+AA+ P+H  +DKGF  MSGT
Sbjct: 491 RTITGKPISSRIAYFSSRGPNSISPGILKPDIAAPGSNIIAAVPPNHKGSDKGFAAMSGT 550

Query: 548 SMAAPHISGIVALLRSLRPTWSPAAIKSALITTARAKSPSGLPIFANGSPPKAADPFDHG 607
           SMAAPHISGIVAL++SLRPTWSPAAIKSALITTAR + PSGLPIFA GSPPK ADPFD+G
Sbjct: 551 SMAAPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPKVADPFDYG 610

Query: 608 GGVVDPNAAIDPGLIYDLDTIEYI-YYLCGMGYKGSDISQLTQQKTECPFPRPSVLDLNL 667
           GGVVD NAA+DPGLIYDL   +YI YYLC MGY  ++IS L+QQKT CP  R SVLDLNL
Sbjct: 611 GGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEAEISHLSQQKTVCPSKRASVLDLNL 670

Query: 668 PTITVPELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVLAFNSKVRKISFKV 727
           PTITVP L NSTTVTRTVTNVGN+T++Y+A+I+APPGSKV V+P+VLAFNS V+KISFKV
Sbjct: 671 PTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLAFNSNVKKISFKV 730

Query: 728 TFSTALQNNYGYSFGSLTWTDGVHLVKTPLTVRIDFF 762
            FS++LQ NYGYSFGSLTWTDGVHLVK+PL+VR DFF
Sbjct: 731 KFSSSLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDFF 757

BLAST of Spg029776 vs. NCBI nr
Match: KAG6577195.1 (Subtilisin-like protease 3.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1033.9 bits (2672), Expect = 7.1e-298
Identity = 528/757 (69.75%), Postives = 614/757 (81.11%), Query Frame = 0

Query: 8   LIVGVMTIYALFSMFAYKPMAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDLLATVLGS 67
           L+V V+TIY +FS      MAEADDQN KVHIVYLGER +DD KLTT SHH+LL +V+GS
Sbjct: 11  LVVAVVTIYVVFS-----AMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHELLESVMGS 70

Query: 68  KEKSSESMVYSYRHGFSGFAAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRSWDFLGLS 127
           KEKS ESMVYSY+HGFSGFAAKLT SQAQKLA +P V RVFPS LYK+ TTRSWDFLGLS
Sbjct: 71  KEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSPLYKMRTTRSWDFLGLS 130

Query: 128 SSPSESSNLLHRAKMGDNVIIGVVDSGYWPESESFGDKGLGPVPSRWKGTCESGEEFNST 187
           SSPS SSNLLHRAKMGDNVIIGV+D+G+WPESESF DKG+GP+PSRWKG C+SGE+FNS+
Sbjct: 131 SSPSASSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGEDFNSS 190

Query: 188 NCNRKVIGARWYMKGFVADNGREALADEYLSPRDINGHGTHTASTAAGSFVTNVSYHGLG 247
           +CN+KVIGARW+    VAD+G EA+  +YLS RD  GHGTHTASTA G+FV NVS+ G G
Sbjct: 191 HCNKKVIGARWFASALVADHGEEAVFMDYLSARDNEGHGTHTASTAGGAFVRNVSFFGNG 250

Query: 248 VGTLRGGAPLARLAIYKALWTSDCVGSAADILKAIDEAIHDGVDVLSISIGRFIPLFPEF 307
            GTLRGGAPLARLAIYK LW+   +GS ADILK IDEAIHDGVDVLS+SIG+ IPLF + 
Sbjct: 251 RGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIGKSIPLFSDV 310

Query: 308 NEENEIAIGSFHAVARGISVVCAGGNDGPLQQTVQNTAPWILTVAASTMDRAFLSSI-TL 367
           NE N +A+GSFHA+A+GISVVC+GGN+G +QQTV+N APW+ TVAAST+DRAFL+SI TL
Sbjct: 311 NELNPVAVGSFHAIAKGISVVCSGGNEGSIQQTVENVAPWLFTVAASTIDRAFLASITTL 370

Query: 368 PDNTTYLGQSFYDSEKELVAKLTTASNGRCSDILGNETFISGKVV-LCFSDIADKTAANM 427
            DN TYLGQ+F   +K++V  L  A +GRC+ +LG+   ISG VV LCF+D+A K  A+ 
Sbjct: 371 GDNATYLGQTFL--KKDIVGML-LAMDGRCAGLLGSNIHISGNVVLLCFTDLAKKAGASN 430

Query: 428 AAAAAVKANGSGIIVAGQQDDDLFACKQPFPCILVDNTVGTKLLFHVLEGSNPMVRLGRA 487
                 +A   GII AGQ +D L  C    PCI VD  VGTKL  + L      +R+   
Sbjct: 431 VVMPGKQAKVVGIIYAGQHNDILGPCDT--PCIHVDTHVGTKLFTYYLNDEKAFIRVRAT 490

Query: 488 KTIIGKPISTRIVYFSSRGPNSASPAILKRDIAAPGSNILAAISPHHSSNDKGFGMMSGT 547
           +TI GKPIS+RI YFSSRGPNS SP ILK DIAAPG+NI+AA+ P+H   DKGF +MSGT
Sbjct: 491 RTITGKPISSRIAYFSSRGPNSISPGILKPDIAAPGANIIAAVPPNHEGGDKGFAVMSGT 550

Query: 548 SMAAPHISGIVALLRSLRPTWSPAAIKSALITTARAKSPSGLPIFANGSPPKAADPFDHG 607
           SMAAPHISGIVAL++SLRPTWSPAAIKSALITTAR +  SGLPIFA GSPPK AD FD+G
Sbjct: 551 SMAAPHISGIVALIKSLRPTWSPAAIKSALITTARVEDLSGLPIFAEGSPPKVADSFDYG 610

Query: 608 GGVVDPNAAIDPGLIYDLDTIEYI-YYLCGMGYKGSDISQLTQQKTECPFPRPSVLDLNL 667
           GGVVD NAAIDPGLIYDL+  +YI YYLC MGY  +DIS L+QQKT CP  R S+LDLNL
Sbjct: 611 GGVVDANAAIDPGLIYDLEVTDYIYYYLCSMGYTEADISHLSQQKTVCPSKRTSILDLNL 670

Query: 668 PTITVPELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVLAFNSKVRKISFKV 727
           PTITVP L NSTTVTRTVTNVGN+T++Y+A+I+APPGSKV V+P+VL FNS V+KISFKV
Sbjct: 671 PTITVPTLTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKV 730

Query: 728 TFSTALQNNYGYSFGSLTWTDGVHLVKTPLTVRIDFF 762
            FS+ LQ NYGYSFGSLTWTDGVHLVK+PL+VR DFF
Sbjct: 731 KFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDFF 757

BLAST of Spg029776 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 3.7e-225
Identity = 406/759 (53.49%), Postives = 527/759 (69.43%), Query Frame = 0

Query: 14  TIYALFSMFAYKPMAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDLLATVLGSKEKSSE 73
           ++  + S+     +A A  ++ KVHIVYLGE+QHDD K  T+SHH +L+++LGSK+ + E
Sbjct: 7   SVLVVLSLIIVLNVARASAKS-KVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHE 66

Query: 74  SMVYSYRHGFSGFAAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRSWDFLGLSSSPSES 133
           SMVYSYRHGFSGFAAKLT+SQA+K+A+ PEVI V P S Y+L TTR WD+LG   S   S
Sbjct: 67  SMVYSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYELATTRIWDYLG--PSADNS 126

Query: 134 SNLLHRAKMGDNVIIGVVDSGYWPESESFGDKGLGPVPSRWKGTCESGEEFNSTNCNRKV 193
            NL+    MGD  IIGV+D+G WPESESF D G+GPVPS WKG CE GE F STNCNRK+
Sbjct: 127 KNLVSDTNMGDQTIIGVIDTGVWPESESFNDYGVGPVPSHWKGGCEPGENFISTNCNRKL 186

Query: 194 IGARWYMKGFVADNGREAL-ADEYLSPRDINGHGTHTASTAAGSFVTNVSYHGLGVGTLR 253
           IGA++++ GF+A+N   A  + +Y+S RD +GHGTH AS A GSFV NVSY GLG GTLR
Sbjct: 187 IGAKYFINGFLAENQFNATESPDYISARDFDGHGTHVASIAGGSFVPNVSYKGLGRGTLR 246

Query: 254 GGAPLARLAIYKALW----TSDCVGSAADILKAIDEAIHDGVDVLSISIGRFIPLFPEFN 313
           GGAP AR+A+YKA W          S +DI+KAIDEAIHDGVDVLSIS+G  +PL  E +
Sbjct: 247 GGAPRARIAMYKACWYINELDGVTCSFSDIMKAIDEAIHDGVDVLSISLGGRVPLNSETD 306

Query: 314 EENEIAIGSFHAVARGISVVCAGGNDGPLQQTVQNTAPWILTVAASTMDRAFLSSITLPD 373
             + IA G+FHAVA+GI VVCAGGN GP  QTV NTAPWILTVAA+T+DR+F + I L +
Sbjct: 307 LRDGIATGAFHAVAKGIVVVCAGGNAGPSSQTVVNTAPWILTVAATTLDRSFATPIILGN 366

Query: 374 NTTYLGQSFYDSEK---------ELVAKLTTASNGRCSDI-LGNETFISGKVVLCFSDIA 433
           N   LGQ+ Y   +         E         +G C  + L +   ++GKVVLCF+   
Sbjct: 367 NQVILGQAMYIGPELGFTSLVYPEDPGNSIDTFSGVCESLNLNSNRTMAGKVVLCFTTAR 426

Query: 434 DKTAANMAAAAAVKANGSGIIVAGQQDDDLFACKQPFPCILVDNTVGTKLLFHVLEGSNP 493
           D T  + AA+    A G G+I+A     +L  C   FPC+ +DN +GT +LF++    +P
Sbjct: 427 DFTVVSTAASIVKAAGGLGLIIARNPGYNLAPCSDDFPCVAIDNELGTDILFYIRYTGSP 486

Query: 494 MVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKRDIAAPGSNILAAISPHHSSNDKG 553
           +V++  ++T++G+P+ T++  FSSRGPNS SPAILK DIAAPG +ILAA SP+ + N  G
Sbjct: 487 VVKIQPSRTLVGEPVGTKVATFSSRGPNSISPAILKPDIAAPGVSILAATSPNDTLNAGG 546

Query: 554 FGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITTARAKSPSGLPIFANGSPPKA 613
           F M SGTSMAAP ISG++ALL+SL P WSPAA +SA++TTA    P G  I A  S  K 
Sbjct: 547 FVMRSGTSMAAPVISGVIALLKSLHPDWSPAAFRSAIVTTAWRTDPFGEQIAAESSSLKV 606

Query: 614 ADPFDHGGGVVDPNAAIDPGLIYDLDTIEYIYYLCGMGYKGSDISQLTQQKTECPFPRPS 673
            DPFD+GGG+V+P  A +PGLI D+D+ +Y+ YLC  GY  S IS+L  + T C  P+PS
Sbjct: 607 PDPFDYGGGLVNPEKAAEPGLILDMDSQDYVLYLCSAGYNDSSISRLVGKVTVCSNPKPS 666

Query: 674 VLDLNLPTITVPELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVLAFNSKVR 733
           VLD+NLP+IT+P L++  T+TRTVTNVG V S+Y+ ++E P G +V V P+ L FNSK +
Sbjct: 667 VLDINLPSITIPNLKDEVTLTRTVTNVGPVDSVYKVLVEPPLGIQVVVTPETLVFNSKTK 726

Query: 734 KISFKVTFSTALQNNYGYSFGSLTWTDGVHLVKTPLTVR 758
            +SF V  ST  + N G+ FGSLTWTD +H V  P++VR
Sbjct: 727 SVSFTVIVSTTHKINTGFYFGSLTWTDSIHNVVIPVSVR 762

BLAST of Spg029776 vs. ExPASy Swiss-Prot
Match: Q9SZY2 (Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 SV=2)

HSP 1 Score: 778.9 bits (2010), Expect = 5.4e-224
Identity = 401/774 (51.81%), Postives = 538/774 (69.51%), Query Frame = 0

Query: 1   MVNSQGRLIVGVMTIYALFSMFAYKPMAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDL 60
           M N +  + V +  +  L     + P A A+    KVHIVYLGE+QHDD +  T+SHH +
Sbjct: 1   MRNHRTSIFVVLSLVIILNGQSGFLPRAGAES---KVHIVYLGEKQHDDPEFVTESHHRM 60

Query: 61  LATVLGSKEKSSESMVYSYRHGFSGFAAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRS 120
           L ++LGSKE++  SMV+S+RHGFSGFAAKLT SQA+K+A++PEV+ V P   YK  TTR+
Sbjct: 61  LWSLLGSKEEAHGSMVHSFRHGFSGFAAKLTESQAKKIADLPEVVHVIPDRFYKPATTRT 120

Query: 121 WDFLGLSSSPSESSNLLHRAKMGDNVIIGVVDSGYWPESESFGDKGLGPVPSRWKGTCES 180
           WD+LGL  SP+   NLL++  MG+ +IIG++DSG WPESE F D  +GPVPS WKG CES
Sbjct: 121 WDYLGL--SPTNPKNLLNQTNMGEQMIIGIIDSGVWPESEVFNDNEIGPVPSHWKGGCES 180

Query: 181 GEEFNSTNCNRKVIGARWYMKGFVAD----NGREALADEYLSPRDINGHGTHTASTAAGS 240
           GE+FNS++CN+K+IGA++++  F+A     N  E+L  +++SPR  NGHGTH A+ A GS
Sbjct: 181 GEDFNSSHCNKKLIGAKYFINAFLATHESFNSSESL--DFISPRGYNGHGTHVATIAGGS 240

Query: 241 FVTNVSYHGLGVGTLRGGAPLARLAIYKALWTSD---CVGSAADILKAIDEAIHDGVDVL 300
           +V N SY GL  GT+RGGAP AR+A+YK  W  D      S+ADILKA+DEAIHDGVDVL
Sbjct: 241 YVPNTSYKGLAGGTVRGGAPRARIAVYKTCWYLDLDIAACSSADILKAMDEAIHDGVDVL 300

Query: 301 SISIGRFIPLFPEFNEENEIAIGSFHAVARGISVVCAGGNDGPLQQTVQNTAPWILTVAA 360
           S+S+G F PL+PE +  + IA G+FHAV +GI+VVCA GN GP  QTV NTAPWILTVAA
Sbjct: 301 SLSLG-FEPLYPETDVRDGIATGAFHAVLKGITVVCAAGNAGPAAQTVGNTAPWILTVAA 360

Query: 361 STMDRAFLSSITLPDNTTYLGQSFYDSEKELVAKLTTASN---------GRCSDILGNET 420
           +T+DR+F++ +TL +N   LGQ+ Y   +     L    N         G C  +L N  
Sbjct: 361 TTLDRSFVTPMTLGNNKVILGQAIYTGTEVGFTSLVYPENPGNSNESFSGTCERLLINSN 420

Query: 421 -FISGKVVLCFSDIADKTAANMAAAAAVKANGSGIIVAGQQDDDLFACKQPFPCILVDNT 480
             ++GKVVLCF++     +   AA    +A G G+I+AGQ  + L  C   FPC+ VD  
Sbjct: 421 RTMAGKVVLCFTESPYSISVTRAAHYVKRAGGLGVIIAGQPGNVLRPCLDDFPCVAVDYE 480

Query: 481 VGTKLLFHVLEGSNPMVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKRDIAAPGSN 540
           +GT +LF++    +P+V++  ++T+IG+P+ T++  FSSRGPN  S AILK DIAAPG +
Sbjct: 481 LGTYILFYIRSNGSPVVKIQPSRTLIGQPVGTKVASFSSRGPNPISAAILKPDIAAPGVS 540

Query: 541 ILAAISPHHSSNDKGFGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITTARAKS 600
           ILAA + + + ND+GF  +SGTSMA P ISGIVALL++L P WSPAAI+SA++TTA    
Sbjct: 541 ILAATTTNTTFNDRGFIFLSGTSMATPTISGIVALLKALHPDWSPAAIRSAIVTTAWRTD 600

Query: 601 PSGLPIFANGSPPKAADPFDHGGGVVDPNAAIDPGLIYDLDTIEYIYYLCGMGYKGSDIS 660
           P G  IFA GSP K ADPFD+GGG+V+P  A  PGL+YDL   +Y+ Y+C +GY  + IS
Sbjct: 601 PFGEQIFAEGSPRKPADPFDYGGGLVNPEKATKPGLVYDLGLEDYVLYMCSVGYNETSIS 660

Query: 661 QLTQQKTECPFPRPSVLDLNLPTITVPELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSK 720
           QL  + T C +P+PSVLD NLP+IT+P L+   T+ RT+TNVG + S+YR  +E P G++
Sbjct: 661 QLVGKGTVCSYPKPSVLDFNLPSITIPNLKEEVTLPRTLTNVGPLESVYRVAVEPPLGTQ 720

Query: 721 VTVEPQVLAFNSKVRKISFKVTFSTALQNNYGYSFGSLTWTDGVHLVKTPLTVR 758
           VTV P+ L FNS  +++SFKV+ ST  + N GY FGSLTW+D +H V  PL+VR
Sbjct: 721 VTVTPETLVFNSTTKRVSFKVSVSTTHKINTGYYFGSLTWSDSLHNVTIPLSVR 766

BLAST of Spg029776 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 9.2e-224
Identity = 397/747 (53.15%), Postives = 527/747 (70.55%), Query Frame = 0

Query: 27  MAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDLLATVLGSKEKSSESMVYSYRHGFSGF 86
           +  A D++ KVHIVYLGE+QHDD +  ++SHH +L+++LGSK  + ESMVYSYRHGFSGF
Sbjct: 20  VVRASDES-KVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAHESMVYSYRHGFSGF 79

Query: 87  AAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRSWDFLGLSSSPSESSNLLHRAKMGDNV 146
           AAKLT SQA+KLA+ PEV+ V   S Y+L TTR+WD+LGL  S +  +NLL+   MGD V
Sbjct: 80  AAKLTESQAKKLADSPEVVHVMADSFYELATTRTWDYLGL--SVANPNNLLNDTNMGDQV 139

Query: 147 IIGVVDSGYWPESESFGDKGLGPVPSRWKGTCESGEEFNSTNCNRKVIGARWYMKGFVAD 206
           IIG +D+G WPESESF D G+GP+PS WKG CESGE+F STNCNRK+IGA++++ GF+A+
Sbjct: 140 IIGFIDTGVWPESESFNDNGVGPIPSHWKGGCESGEKFISTNCNRKLIGAKYFINGFLAE 199

Query: 207 NG--REALADEYLSPRDINGHGTHTASTAAGSFVTNVSYHGLGVGTLRGGAPLARLAIYK 266
           N       + +Y+S RD  GHGTHTAS A GSFV N+SY GL  G LRGGAP AR+AIYK
Sbjct: 200 NEGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISYKGLAGGNLRGGAPRARIAIYK 259

Query: 267 ALWTSDCVG----SAADILKAIDEAIHDGVDVLSISIGRFIPLFPEFNEENEIAIGSFHA 326
           A W  D +G    S++DILKA+DE++HDGVDVLS+S+G  IPL+PE +  + IA G+FHA
Sbjct: 260 ACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPETDLRDRIATGAFHA 319

Query: 327 VARGISVVCAGGNDGPLQQTVQNTAPWILTVAASTMDRAFLSSITLPDNTTYLGQSFYDS 386
           VA+GI VVCAGGN GP  QTV NTAPWI+TVAA+T+DR+F + ITL +    LGQ+ Y  
Sbjct: 320 VAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPITLGNRKVILGQALYTG 379

Query: 387 EKELVAKLTTASN---------GRCSDI-LGNETFISGKVVLCFSDIADKTAANMAAAAA 446
           ++     L    N         G C  + L     ++GKVVLCF+     TA + AA+  
Sbjct: 380 QELGFTSLVYPENAGFTNETFSGVCERLNLNPNRTMAGKVVLCFTTNTLFTAVSRAASYV 439

Query: 447 VKANGSGIIVAGQQDDDLFACKQPFPCILVDNTVGTKLLFHVLEGSNPMVRLGRAKTIIG 506
             A G G+I+A     +L  C+  FPC+ +D  +GT +L ++    +P+V++  ++T++G
Sbjct: 440 KAAGGLGVIIARNPGYNLTPCRDDFPCVAIDYELGTDVLLYIRSTRSPVVKIQPSRTLVG 499

Query: 507 KPISTRIVYFSSRGPNSASPAILKRDIAAPGSNILAAISPHHSSNDKGFGMMSGTSMAAP 566
           +P+ T++  FSSRGPNS SPAILK DI APG +ILAA SP  +S+  GF +++GTSMAAP
Sbjct: 500 QPVGTKVATFSSRGPNSISPAILKPDIGAPGVSILAATSPDSNSSVGGFDILAGTSMAAP 559

Query: 567 HISGIVALLRSLRPTWSPAAIKSALITTARAKSPSGLPIFANGSPPKAADPFDHGGGVVD 626
            ++G+VALL++L P WSPAA +SA++TTA    P G  IFA GS  K ADPFD+GGG+V+
Sbjct: 560 VVAGVVALLKALHPNWSPAAFRSAIVTTAWRTDPFGEQIFAEGSSRKVADPFDYGGGIVN 619

Query: 627 PNAAIDPGLIYDLDTIEYIYYLCGMGYKGSDISQLTQQKTECPFPRPSVLDLNLPTITVP 686
           P  A DPGLIYD+   +YI YLC  GY  S I+QL    T C  P+ SVLD+NLP+IT+P
Sbjct: 620 PEKAADPGLIYDMGPRDYILYLCSAGYNDSSITQLVGNVTVCSTPKTSVLDVNLPSITIP 679

Query: 687 ELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVLAFNSKVRKISFKVTFSTAL 746
           +L++  T+TRTVTNVG V S+Y+ ++E P G +V V P+ L FNSK + +SF V  ST  
Sbjct: 680 DLKDEVTLTRTVTNVGTVDSVYKVVVEPPLGIQVVVAPETLVFNSKTKNVSFTVRVSTTH 739

Query: 747 QNNYGYSFGSLTWTDGVHLVKTPLTVR 758
           + N G+ FG+L WTD +H V  P++VR
Sbjct: 740 KINTGFYFGNLIWTDSMHNVTIPVSVR 763

BLAST of Spg029776 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 5.9e-223
Identity = 394/766 (51.44%), Postives = 533/766 (69.58%), Query Frame = 0

Query: 14  TIYALFSMFAY----KPMAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDLLATVLGSKE 73
           +IY + S+  +    +         RKVHIVYLGE+QHDD +  T+SHH +L ++LGSKE
Sbjct: 7   SIYVVLSLVIFLNVQRSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKE 66

Query: 74  KSSESMVYSYRHGFSGFAAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRSWDFLGLSSS 133
            +++SMVYSYRHGFSGFAAKLT SQA+K+A++P+V+ V P S YKL TTR+WD+LGLS++
Sbjct: 67  DANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAA 126

Query: 134 PSESSNLLHRAKMGDNVIIGVVDSGYWPESESFGDKGLGPVPSRWKGTCESGEEFNSTNC 193
             +S  LLH   MG+ +IIGV+D+G WPESE F D G GPVPS WKG CE+GE FNS+NC
Sbjct: 127 NPKS--LLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNC 186

Query: 194 NRKVIGARWYMKGFVADNG--REALADEYLSPRDINGHGTHTASTAAGSFVTNVSYHGLG 253
           N+K+IGA++++ GF+A+N       + +++SPRD++GHGTH ++ A GSFV N+SY GL 
Sbjct: 187 NKKLIGAKYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLA 246

Query: 254 VGTLRGGAPLARLAIYKALW------TSDCVGSAADILKAIDEAIHDGVDVLSISIGRFI 313
            GT+RGGAP A +A+YKA W      T+ C  S+ADILKA+DEA+HDGVDVLSIS+G  +
Sbjct: 247 GGTVRGGAPRAHIAMYKACWYLDDDDTTTC--SSADILKAMDEAMHDGVDVLSISLGSSV 306

Query: 314 PLFPEFNEENEIAIGSFHAVARGISVVCAGGNDGPLQQTVQNTAPWILTVAASTMDRAFL 373
           PL+ E +  + I  G+FHAV +GI+VVC+GGN GP   TV NTAPWI+TVAA+T+DR+F 
Sbjct: 307 PLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFA 366

Query: 374 SSITLPDNTTYLGQSFYDSEKELVAKLTTASN---------GRCSDILGNET-FISGKVV 433
           + +TL +N   LGQ+ Y         L    N         G C ++L N    + GKVV
Sbjct: 367 TPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVV 426

Query: 434 LCFSDIADKTAANMAAAAAVKANGSGIIVAGQQDDDLFACKQPFPCILVDNTVGTKLLFH 493
           LCF+      A   AA    +A G G+I+A      +  C   FPC+ VD  +GT +L +
Sbjct: 427 LCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLY 486

Query: 494 VLEGSNPMVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKRDIAAPGSNILAAISPH 553
                +P+V++  +KT++G+P+ T++  FSSRGPNS +PAILK DIAAPG +ILAA + +
Sbjct: 487 TRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAA-TTN 546

Query: 554 HSSNDKGFGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITTARAKSPSGLPIFA 613
            + +D+GF M+SGTSMAAP ISG+ ALL++L   WSPAAI+SA++TTA    P G  IFA
Sbjct: 547 TTFSDQGFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFA 606

Query: 614 NGSPPKAADPFDHGGGVVDPNAAIDPGLIYDLDTIEYIYYLCGMGYKGSDISQLTQQKTE 673
            GSPPK ADPFD+GGG+V+P  + +PGL+YD+   +Y+ Y+C +GY  + ISQL  + T 
Sbjct: 607 EGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIGKTTV 666

Query: 674 CPFPRPSVLDLNLPTITVPELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVL 733
           C  P+PSVLD NLP+IT+P L++  T+TRTVTNVG + S+YR  +E P G +VTV P+ L
Sbjct: 667 CSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETL 726

Query: 734 AFNSKVRKISFKVTFSTALQNNYGYSFGSLTWTDGVHLVKTPLTVR 758
            FNS  +K+ FKV  ST  + N GY FGSLTW+D +H V  PL+VR
Sbjct: 727 VFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVR 767

BLAST of Spg029776 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 7.8e-223
Identity = 400/729 (54.87%), Postives = 521/729 (71.47%), Query Frame = 0

Query: 36  KVHIVYLGERQHDDTKLTTQSHHDLLATVLGSKEKSSESMVYSYRHGFSGFAAKLTRSQA 95
           KV++VYLGE++HD+ +  T+SHH +L ++LGSKE   +S+VYSYRHGFSGFAAKLT SQA
Sbjct: 28  KVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKLTESQA 87

Query: 96  QKLAEIPEVIRVFPSSLYKLHTTRSWDFLGLSSSPSESSNLLHRAKMGDNVIIGVVDSGY 155
           Q+++E+PEV++V P++LY++ TTR+WD+LG+  SP  S +LL +A MG NVI+GV+DSG 
Sbjct: 88  QQISELPEVVQVIPNTLYEMTTTRTWDYLGV--SPGNSDSLLQKANMGYNVIVGVIDSGV 147

Query: 156 WPESESFGDKGLGPVPSRWKGTCESGEEFN-STNCNRKVIGARWYMKGFVADNG--REAL 215
           WPESE F DKG GP+PSRWKG CESGE FN S +CNRK+IGA++++ G VA+ G      
Sbjct: 148 WPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFGVVNRTQ 207

Query: 216 ADEYLSPRDINGHGTHTASTAAGSFVTNVSYHGLGVGTLRGGAPLARLAIYKALWTSDCV 275
             EYLSPRD  GHGTH AST  GSF+ NVSY GLG GT RGGAP   +A+YKA W+  C 
Sbjct: 208 NPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKACWSGYC- 267

Query: 276 GSAADILKAIDEAIHDGVDVLSISIGRFIPLFPEFNEENEIAIGSFHAVARGISVVCAGG 335
            S AD+LKA+DEAIHDGVD+LS+S+G  +PLFP   E    ++G+FHAVA+GI VV A G
Sbjct: 268 -SGADVLKAMDEAIHDGVDILSLSLGPSVPLFP---ETEHTSVGAFHAVAKGIPVVIAAG 327

Query: 336 NDGPLQQTVQNTAPWILTVAASTMDRAFLSSITLPDNTTYLGQSFYDSEKELVAKLT--- 395
           N GP  QT+ N APW+LTVAA+T DR+F ++ITL +N T LGQ+ Y   +     LT   
Sbjct: 328 NAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIYGGPELGFVGLTYPE 387

Query: 396 TASNGRCSDILGN-ETFISGKVVLCFSDIADKTAANMAAAAAVKANGSGIIVAGQQDDDL 455
           +  +G C  +  N  + + GKVVLCF   A  T +N A AA + A G G+I+A      L
Sbjct: 388 SPLSGDCEKLSANPNSTMEGKVVLCF---AASTPSNAAIAAVINAGGLGLIMAKNPTHSL 447

Query: 456 FACKQPFPCILVDNTVGTKLLFHVLEGSNPMVRLGRAKTIIGKPISTRIVYFSSRGPNSA 515
              ++ FP + +D  +GT +LF++    +P+V++  +KT+ G+ +ST++  FSSRGPNS 
Sbjct: 448 TPTRK-FPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSVSTKVATFSSRGPNSV 507

Query: 516 SPAILKRDIAAPGSNILAAISPHHSSNDKGFGMMSGTSMAAPHISGIVALLRSLRPTWSP 575
           SPAILK DIAAPG NILAAISP+ S ND GF MMSGTSMA P +SG+V LL+SL P WSP
Sbjct: 508 SPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLLKSLHPDWSP 567

Query: 576 AAIKSALITTARAKSPSGLPIFANGSPPKAADPFDHGGGVVDPNAAIDPGLIYDLDTIEY 635
           +AIKSA++TTA    PSG PIFA+GS  K ADPFD+GGG+++P  A+ PGLIYD+ T +Y
Sbjct: 568 SAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLIYDMTTDDY 627

Query: 636 IYYLCGMGYKGSDISQLTQQKTECPFPRPSVLDLNLPTITVPELRNSTTVTRTVTNVGNV 695
           + Y+C + Y    IS++  + T CP P+PSVLDLNLP+IT+P LR   T+TRTVTNVG V
Sbjct: 628 VMYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTLTRTVTNVGPV 687

Query: 696 TSIYRAMIEAPPGSKVTVEPQVLAFNSKVRKISFKVTFSTALQNNYGYSFGSLTWTDGVH 755
            S+Y+ +I+ P G  V V P  L F+    K SF V  ST  + N GY FGSLTWTD +H
Sbjct: 688 NSVYKVVIDPPTGINVAVTPAELVFDYTTTKRSFTVRVSTTHKVNTGYYFGSLTWTDNMH 745

Query: 756 LVKTPLTVR 758
            V  P++VR
Sbjct: 748 NVAIPVSVR 745

BLAST of Spg029776 vs. ExPASy TrEMBL
Match: A0A0A0KWS6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G047950 PE=3 SV=1)

HSP 1 Score: 1087.8 bits (2812), Expect = 0.0e+00
Identity = 547/762 (71.78%), Postives = 632/762 (82.94%), Query Frame = 0

Query: 6   GRLIVGVMTIYALFSMFAYKP-MAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDLLATV 65
           G LIVG +    LF     KP +AEADDQN KVHIVYLGE+ H DTK T  SHH LL+T+
Sbjct: 8   GHLIVGFIIFDCLF-----KPILAEADDQNPKVHIVYLGEKPHHDTKFTIDSHHQLLSTI 67

Query: 66  LGSKEKSSESMVYSYRHGFSGFAAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRSWDFL 125
           LGSKEKS E+MVYSY+HGFSGFAAKLT+SQAQKL+E+  V+RV PSSLYK+HTTRSWDFL
Sbjct: 68  LGSKEKSMEAMVYSYKHGFSGFAAKLTKSQAQKLSEMSRVVRVVPSSLYKVHTTRSWDFL 127

Query: 126 GLSSSPSESSNLLHRAKMGDNVIIGVVDSGYWPESESFGDKGLGPVPSRWKGTCESGEEF 185
           GLSSSP ESSNLLHRA+MG+NVIIGV+D+G WPESESF DKG+G +PSRWKGTCESGE+F
Sbjct: 128 GLSSSPFESSNLLHRAQMGENVIIGVIDTGIWPESESFKDKGVGSIPSRWKGTCESGEQF 187

Query: 186 NSTNCNRKVIGARWYMKGFVADNGREALADEYLSPRDINGHGTHTASTAAGSFVTNVSYH 245
           NSTNCN+K+IGARW+MKGFVAD GR+ALA EYLSPRD+NGHGTHTAS AAGSFV N++YH
Sbjct: 188 NSTNCNKKIIGARWFMKGFVADLGRDALAKEYLSPRDLNGHGTHTASIAAGSFVANINYH 247

Query: 246 GLGVGTLRGGAPLARLAIYKALWTSDCVGSAADILKAIDEAIHDGVDVLSISIGRFIPLF 305
               GT+RGGAPLARLAIYKALWT D VGS ADILKAIDEAI+DGVDVLS+SIG   P  
Sbjct: 248 NNAAGTVRGGAPLARLAIYKALWTKDAVGSTADILKAIDEAINDGVDVLSMSIGSLTPFL 307

Query: 306 PEFNEENEIAIGSFHAVARGISVVCAGGNDGPLQQTVQNTAPWILTVAASTMDRAFLSSI 365
           PEFNE N+IA GSFHA+A+GISVVCA GN GP  QTV+N APWI TVAA+T+DRAFL+SI
Sbjct: 308 PEFNEANDIAFGSFHAIAKGISVVCAAGNSGPTPQTVENVAPWIFTVAANTIDRAFLASI 367

Query: 366 -TLPDNTTYLGQSFYDSEKELVAKLTTASNGRCSDILGNETFISGKVVLCFSDIADKTAA 425
            TLPDNTT+LGQS  DS+K+LVA+L T   GRC D+LGNETFI+GKVV+CFS++AD    
Sbjct: 368 TTLPDNTTFLGQSLLDSKKDLVAELETLDTGRCDDLLGNETFINGKVVMCFSNLADHNTI 427

Query: 426 NMAAAAAVKANGSGIIVAGQQDDDLFAC-KQPFPCILVDNTVGTKLLF-HVLEGS-NPMV 485
             AA A  +ANG+GIIVAGQQDDDLF+C   P PCILVD  VG+KL F ++L+ S NP+V
Sbjct: 428 YDAAMAVARANGTGIIVAGQQDDDLFSCIPSPIPCILVDTDVGSKLFFINLLQNSTNPVV 487

Query: 486 RLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKRDIAAPGSNILAAISPHHSSNDKGFG 545
           RL   +TIIGKPI+  I YFSSRGPNS S  ILK DI+APGSNILAA+SPHH  N+KGF 
Sbjct: 488 RLRATRTIIGKPITPAISYFSSRGPNSVSNPILKPDISAPGSNILAAVSPHHIFNEKGFM 547

Query: 546 MMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITTARAKSPSGLPIFANGSPPKAAD 605
           ++SGTSMA PHIS IVALL+S+ PTWSPAAIKSAL+TTAR +   GLPIFA G+PPK AD
Sbjct: 548 LLSGTSMATPHISAIVALLKSVHPTWSPAAIKSALMTTARTEVSPGLPIFAEGTPPKMAD 607

Query: 606 PFDHGGGVVDPNAAIDPGLIYDLDTIEYI-YYLCGMGYKGSDISQLTQQKTECPFPRPSV 665
           PFD+GGG+VD NAA+DPGL+YD+   +YI YYLCGMGYK  DIS LTQ+KT CP  R SV
Sbjct: 608 PFDYGGGIVDANAAVDPGLVYDMGRKDYIDYYLCGMGYKDEDISHLTQRKTVCPLQRLSV 667

Query: 666 LDLNLPTITVPELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVLAFNSKVRK 725
           LDLNLP IT+P L NST VTRTVTNVGN++ +Y+A IE+P G KV+V PQVL FNS+V+K
Sbjct: 668 LDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAEIESPFGCKVSVNPQVLVFNSQVKK 727

Query: 726 ISFKVTFSTALQNNYGYSFGSLTWTDGVHLVKTPLTVRIDFF 762
           ISFKV F T +Q NYGYSFG LTWTDG+H+VK PL+VR  FF
Sbjct: 728 ISFKVMFFTQVQRNYGYSFGRLTWTDGIHVVKIPLSVRFGFF 764

BLAST of Spg029776 vs. ExPASy TrEMBL
Match: A0A6J1JBL4 (subtilisin-like protease SBT3.9 OS=Cucurbita maxima OX=3661 GN=LOC111482985 PE=3 SV=1)

HSP 1 Score: 1047.7 bits (2708), Expect = 2.3e-302
Identity = 533/762 (69.95%), Postives = 616/762 (80.84%), Query Frame = 0

Query: 3   NSQGRLIVGVMTIYALFSMFAYKPMAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDLLA 62
           N     I+ V+TIYA+FS      MAEADDQN KVHIVYLGER +DD KLTT SHH+LL 
Sbjct: 4   NRAPSFILAVVTIYAVFS-----DMAEADDQNSKVHIVYLGERPYDDVKLTTDSHHELLE 63

Query: 63  TVLGSKEKSSESMVYSYRHGFSGFAAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRSWD 122
           +VLGSKEKS ESMVYSYRHGFSGFAAKLT SQAQKLA +P V RVFPSSLYK+ TTRSWD
Sbjct: 64  SVLGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTRSWD 123

Query: 123 FLGLSSSPSESSNLLHRAKMGDNVIIGVVDSGYWPESESFGDKGLGPVPSRWKGTCESGE 182
           FLGLSSSPSESSNL HRAKMGDNVIIGV+D+G+WPESESF DKG+GP+PSRWKG C+SGE
Sbjct: 124 FLGLSSSPSESSNLFHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGE 183

Query: 183 EFNSTNCNRKVIGARWYMKGFVADNGREALADEYLSPRDINGHGTHTASTAAGSFVTNVS 242
           +FNS++CN+KVIGARW+    +AD+G EA+  +YLS RD  GHGTHTASTA G+FV NVS
Sbjct: 184 DFNSSHCNKKVIGARWFASALIADHGEEAVFKDYLSARDNEGHGTHTASTAGGAFVRNVS 243

Query: 243 YHGLGVGTLRGGAPLARLAIYKALWTSDCVGSAADILKAIDEAIHDGVDVLSISIGRFIP 302
           Y G G GTLRGGAPLARLAIYK LW+   +GS ADILK IDEAIHDGVDVLS+SIG+ IP
Sbjct: 244 YFGNGRGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIGKSIP 303

Query: 303 LFPEFNEENEIAIGSFHAVARGISVVCAGGNDGPLQQTVQNTAPWILTVAASTMDRAFLS 362
           LFP+ N+ N +A+GSFHA+A+GISVVCAGGN+G +QQTV+N APW+ TVAAST+DRAFL 
Sbjct: 304 LFPDVNDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFLV 363

Query: 363 SI-TLPDNTTYLGQSFYDSEKELVAKLTTASNGRCSDILGNETFISGKVV-LCFSDIADK 422
           SI TL DN TYLGQ F    K++V  L  A +GRC+ ILG +  ISG VV LCF+D+A  
Sbjct: 364 SITTLGDNATYLGQMFL--RKDIVGML-VAMDGRCAGILGGDIPISGNVVLLCFTDLAKT 423

Query: 423 TAANMAAAAAVKANGSGIIVAGQQDDDLFACKQPFPCILVDNTVGTKLLFHVLEGSNPMV 482
             A+ A     +A   G+I AGQQ D+L  C    PCI VD  VGTKL  ++L   + ++
Sbjct: 424 APASNAVMPGKQAKVVGVIYAGQQTDNLVPC--DIPCIHVDTHVGTKLFTYLLNDDDALI 483

Query: 483 RLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKRDIAAPGSNILAAISPHHSSNDKGFG 542
           RL   +TIIGKPIS+RI YFSSRGPNS SP ILK DIAAPG+NI+AA+ P+H   DKGF 
Sbjct: 484 RLRATRTIIGKPISSRIAYFSSRGPNSFSPGILKPDIAAPGANIIAAVPPNHKGGDKGFA 543

Query: 543 MMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITTARAKSPSGLPIFANGSPPKAAD 602
            MSGTSMA PHISGIV L++SL PTWSPAAIKSALITTAR + PSG+PIFA GSPPK AD
Sbjct: 544 TMSGTSMATPHISGIVTLIKSLHPTWSPAAIKSALITTARVEDPSGMPIFAEGSPPKVAD 603

Query: 603 PFDHGGGVVDPNAAIDPGLIYDLDTIEYI-YYLCGMGYKGSDISQLTQQKTECPFPRPSV 662
           PFD+GGGVVD NAA+DPGLIYDL   +YI YYLC MGY  +DIS L+QQKT CP  R S+
Sbjct: 604 PFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPSKRASI 663

Query: 663 LDLNLPTITVPELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVLAFNSKVRK 722
           LDLNLPTITVP L NSTTVTRTVTNVGN+T++Y+A+I+APPGSKV V+P+VL FNS V+K
Sbjct: 664 LDLNLPTITVPALINSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKK 723

Query: 723 ISFKVTFSTALQNNYGYSFGSLTWTDGVHLVKTPLTVRIDFF 762
           ISFKV FS+ LQ NYGYSFGSLTWTDGVHLVK+PL+VR DFF
Sbjct: 724 ISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDFF 755

BLAST of Spg029776 vs. ExPASy TrEMBL
Match: A0A1S3BU23 (subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493167 PE=3 SV=1)

HSP 1 Score: 1043.1 bits (2696), Expect = 5.6e-301
Identity = 533/766 (69.58%), Postives = 624/766 (81.46%), Query Frame = 0

Query: 2   VNSQ-GRLIVGVMTIYALFSMFAYKPMAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDL 61
           +NSQ   LIVG +  Y L        +AE++DQN KVHIVYLGE+ H DTK TT SHH L
Sbjct: 3   INSQLAHLIVGFIIFYGLCEPI----LAESNDQNPKVHIVYLGEKPHHDTKFTTDSHHQL 62

Query: 62  LATVLGSKEKSSESMVYSYRHGFSGFAAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRS 121
           LA +LGSKEKS E+MVYSY+HGFSGFAAKLT+S+AQKL+E+  V+RV PSSLYK+HTTRS
Sbjct: 63  LAAILGSKEKSLEAMVYSYKHGFSGFAAKLTKSEAQKLSEMSRVVRVVPSSLYKVHTTRS 122

Query: 122 WDFLGLSSSPSESSNLLHRAKMGDNVIIGVVDSGYWPESESFGDKGLGPVPSRWKGTCES 181
           WDFLGLSSSPSESSNLLHRAK GD+VIIGV+DSG WPESESF DKGLGP+PSRWKGTCES
Sbjct: 123 WDFLGLSSSPSESSNLLHRAK-GDDVIIGVIDSGIWPESESFKDKGLGPIPSRWKGTCES 182

Query: 182 GEEFNSTNCNRKVIGARWYMKGFVADNGREALADEYLSPRDINGHGTHTASTAAGSFVTN 241
           GE+FNSTNCN+K+IGARW++K FVAD GREALA EYLSPRD++GHGTHTASTAAGSFV N
Sbjct: 183 GEQFNSTNCNKKIIGARWFVKAFVADYGREALAKEYLSPRDLHGHGTHTASTAAGSFVAN 242

Query: 242 VSYHGLGVGTLRGGAPLARLAIYKALWTSDCVGSAADILKAIDEAIHDGVDVLSISIGRF 301
           ++YH    GT RGGAPLARLAIYKALWT+  VGS+ADILKAIDEAIHDGVDVLSISIG  
Sbjct: 243 INYHNNAAGTARGGAPLARLAIYKALWTNRGVGSSADILKAIDEAIHDGVDVLSISIGGS 302

Query: 302 IPLFPEFNEENEIAIGSFHAVARGISVVCAGGNDGPLQQTVQNTAPWILTVAASTMDRAF 361
            P +PEF E ++IA GSFHA+ +GISVVCA GN GP  Q V N APWI TVA +T+DRAF
Sbjct: 303 PPFYPEFTELSDIAFGSFHAITKGISVVCAAGNSGPSPQMVDNVAPWIFTVATNTIDRAF 362

Query: 362 LSSI-TLPDNTTYLGQSFYDSEKELVAKLTTASNGRCSDILGNETFISGKVVLCFSDIAD 421
           LSSI TL DNTT++GQS  +S+K+LVA+L   S  RC  + GNE FI+GKVVLCF  +AD
Sbjct: 363 LSSITTLRDNTTFMGQSLLESKKDLVAEL--VSWERCDQLSGNEAFINGKVVLCFPKLAD 422

Query: 422 KTAANMAAAAAVKANGSGIIVAGQQ-DDDLFAC-KQPFPCILVDNTVGTKLLFHVLEGS- 481
            +  + AA   V+ANG+GIIVAGQQ D++L AC   P PCILVD  VG+KL F+ L+ S 
Sbjct: 423 YSTISKAAEVVVRANGTGIIVAGQQVDNNLLACISSPIPCILVDTIVGSKLFFYFLQNSD 482

Query: 482 NPMVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKRDIAAPGSNILAAISPHHSSND 541
           +P+V L  A+TIIGKPI+  I +FSSRGPNS SP ILK DI+APGSNIL+A+SPH+  N+
Sbjct: 483 HPVVMLRAARTIIGKPIAPTIAHFSSRGPNSVSPPILKPDISAPGSNILSAVSPHYFFNE 542

Query: 542 KGFGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITTARAKSPSGLPIFANGSPP 601
           KGF +MSGTSMA PH+S IVALL+S+ PTWSPAAIKSAL+TTAR +   GLPIFA+G+PP
Sbjct: 543 KGFSLMSGTSMATPHVSAIVALLKSVHPTWSPAAIKSALMTTARTEVSPGLPIFADGTPP 602

Query: 602 KAADPFDHGGGVVDPNAAIDPGLIYDLDTIEYI-YYLCGMGYKGSDISQLTQQKTECPFP 661
           K ADPFD+G GVVD NAA+DPGLIYD+   +YI YYLCGMGY+  DIS LT +KTECP  
Sbjct: 603 KVADPFDYGAGVVDANAAVDPGLIYDMGRKDYIDYYLCGMGYEDKDISHLTHRKTECPLQ 662

Query: 662 RPSVLDLNLPTITVPELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVLAFNS 721
           R S+LDLNLP IT+P L NST VTRTVTNVGN++ +Y+A IEAP G KV+V PQVL FNS
Sbjct: 663 RLSLLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAKIEAPFGCKVSVNPQVLVFNS 722

Query: 722 KVRKISFKVTFSTALQNNYGYSFGSLTWTDGVHLVKTPLTVRIDFF 762
           +V+KISFKV F T +Q NYGYSFG LTW+DGVH+VK PL+VR  FF
Sbjct: 723 QVKKISFKVMFFTQVQRNYGYSFGRLTWSDGVHVVKIPLSVRFGFF 761

BLAST of Spg029776 vs. ExPASy TrEMBL
Match: A0A6J1EZL0 (subtilisin-like protease SBT3.7 OS=Cucurbita moschata OX=3662 GN=LOC111437655 PE=3 SV=1)

HSP 1 Score: 1026.9 bits (2654), Expect = 4.2e-296
Identity = 526/757 (69.48%), Postives = 610/757 (80.58%), Query Frame = 0

Query: 8   LIVGVMTIYALFSMFAYKPMAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDLLATVLGS 67
           L+V V+TIY +FS      M EADDQN KVHIVYLGER +DD  LTT SHH+LL +VLGS
Sbjct: 11  LVVAVVTIYVVFS-----AMGEADDQNSKVHIVYLGERPYDDVTLTTDSHHELLESVLGS 70

Query: 68  KEKSSESMVYSYRHGFSGFAAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRSWDFLGLS 127
           KEKS ES+VYSYRHGFSGFAAKLT SQAQKLA +P V RVFPS LYK+ TTRSWDFLGLS
Sbjct: 71  KEKSLESIVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSPLYKMRTTRSWDFLGLS 130

Query: 128 SSPSESSNLLHRAKMGDNVIIGVVDSGYWPESESFGDKGLGPVPSRWKGTCESGEEFNST 187
           SSPS SSNLLHRAKMGDNVIIGV+D+G+WPESESF DKG+GP+PSRWKG C+SGE+FNS+
Sbjct: 131 SSPSASSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGEDFNSS 190

Query: 188 NCNRKVIGARWYMKGFVADNGREALADEYLSPRDINGHGTHTASTAAGSFVTNVSYHGLG 247
           +CN+KVIGARW+    VAD+G EA+  +YLS RD  GHGTHTASTA G+FV NVSY G G
Sbjct: 191 HCNKKVIGARWFASALVADHGEEAVFMDYLSARDNEGHGTHTASTAGGAFVRNVSYFGNG 250

Query: 248 VGTLRGGAPLARLAIYKALWTSDCVGSAADILKAIDEAIHDGVDVLSISIGRFIPLFPEF 307
            GTLRGGAPLARLAIYK LW+   +GS ADILK IDEAIHDGVDVLS+SIG+ IPLF + 
Sbjct: 251 RGTLRGGAPLARLAIYKVLWSDSRLGSGADILKGIDEAIHDGVDVLSMSIGKSIPLFSDV 310

Query: 308 NEENEIAIGSFHAVARGISVVCAGGNDGPLQQTVQNTAPWILTVAASTMDRAFLSSI-TL 367
           NE N +A+GSFHA+A+GISVVCAGGN+G +QQTV+N APW+ TVAA+T+DRAFL+SI TL
Sbjct: 311 NELNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAATTIDRAFLASITTL 370

Query: 368 PDNTTYLGQSFYDSEKELVAKLTTASNGRCSDILGNETFISGKVV-LCFSDIADKTAANM 427
            DN TYLGQ+F   +K++V  L  A + RC+ +LG+   ISG VV LCF+D+A K  A+ 
Sbjct: 371 GDNATYLGQTFL--KKDIVGML-LAMDRRCAGLLGSNIPISGNVVLLCFTDLAKKAGASN 430

Query: 428 AAAAAVKANGSGIIVAGQQDDDLFACKQPFPCILVDNTVGTKLLFHVLEGSNPMVRLGRA 487
                  A   GII AGQ +D L  C    PCI VD  VGT+L  + L      +R+   
Sbjct: 431 VVMPGKPAKVVGIIYAGQHNDILGPCDT--PCIHVDTHVGTQLFTYYLNDEKAFIRVRAT 490

Query: 488 KTIIGKPISTRIVYFSSRGPNSASPAILKRDIAAPGSNILAAISPHHSSNDKGFGMMSGT 547
           +TI GKPIS+RI YFSSRGPNS SP ILK DIAAPG+NI+AA+ P+H   DKGF +MSGT
Sbjct: 491 RTITGKPISSRIAYFSSRGPNSISPGILKPDIAAPGANIIAAVPPNHEGGDKGFAVMSGT 550

Query: 548 SMAAPHISGIVALLRSLRPTWSPAAIKSALITTARAKSPSGLPIFANGSPPKAADPFDHG 607
           SMAAPHISGIVAL++SLRPTWSPA IKSALITTAR +  SGLPIFA GSPPK ADPFD+G
Sbjct: 551 SMAAPHISGIVALIKSLRPTWSPAVIKSALITTARVEDLSGLPIFAEGSPPKVADPFDYG 610

Query: 608 GGVVDPNAAIDPGLIYDLDTIEYI-YYLCGMGYKGSDISQLTQQKTECPFPRPSVLDLNL 667
           GGVVD NAAIDPGLIYDL+  +YI YYLC MGY  +DIS L+QQKT CP  R S+LDLNL
Sbjct: 611 GGVVDANAAIDPGLIYDLEVTDYIYYYLCSMGYTEADISHLSQQKTVCPSKRTSILDLNL 670

Query: 668 PTITVPELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVLAFNSKVRKISFKV 727
           PTITVP L NSTTVTRTVTNVGN+T++Y+A+I+APPGSKV V+P+VL FNS V+KISFKV
Sbjct: 671 PTITVPTLTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFNSNVKKISFKV 730

Query: 728 TFSTALQNNYGYSFGSLTWTDGVHLVKTPLTVRIDFF 762
            FS+ LQ NYGYSFGSLTWTDGVHLVK+PL+VR DFF
Sbjct: 731 KFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDFF 757

BLAST of Spg029776 vs. ExPASy TrEMBL
Match: A0A6J1EZK4 (subtilisin-like protease SBT3.7 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 1025.4 bits (2650), Expect = 1.2e-295
Identity = 527/757 (69.62%), Postives = 607/757 (80.18%), Query Frame = 0

Query: 8   LIVGVMTIYALFSMFAYKPMAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDLLATVLGS 67
           L V ++TIYA+FS      MAE DDQN KVHIVYLGER +DD KLTT SHH+LL +VL S
Sbjct: 11  LAVAIVTIYAVFS-----AMAEVDDQNSKVHIVYLGERPYDDVKLTTHSHHELLESVLRS 70

Query: 68  KEKSSESMVYSYRHGFSGFAAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRSWDFLGLS 127
           KEKS ESMVYSY+HGFSGFAAKLT SQAQKLA +P V RVFPSSLYK+HTTRSWDFLGLS
Sbjct: 71  KEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRSWDFLGLS 130

Query: 128 SSPSESSNLLHRAKMGDNVIIGVVDSGYWPESESFGDKGLGPVPSRWKGTCESGEEFNST 187
           SSPS SSNLLHRAKMGDNVIIGV+DSG+WPESESF DKG+GP+PSRWKG C+ GE+FNS 
Sbjct: 131 SSPSASSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQLGEDFNSF 190

Query: 188 NCNRKVIGARWYMKGFVADNGREALADEYLSPRDINGHGTHTASTAAGSFVTNVSYHGLG 247
           +CN+KVIGARW+ +  +AD+G EA+  +YLS RD  GHGTHTASTA G+FV NVSY G  
Sbjct: 191 HCNKKVIGARWFARALIADHGEEAVFKDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNR 250

Query: 248 VGTLRGGAPLARLAIYKALWTSDCVGSAADILKAIDEAIHDGVDVLSISIGRFIPLFPEF 307
            GTLRGGAPLARLAIYK +W+   +GSAADILK IDEAIHDGVDVLS+SIG  IPL P+ 
Sbjct: 251 RGTLRGGAPLARLAIYKVIWSDRRLGSAADILKGIDEAIHDGVDVLSMSIGGSIPLPPDV 310

Query: 308 NEENEIAIGSFHAVARGISVVCAGGNDGPLQQTVQNTAPWILTVAASTMDRAFLSSI-TL 367
           +E N +AIGSFHA+ARG+SVVCAGGN+G +QQTV N APW+ TVAAST+DRAFL+SI TL
Sbjct: 311 SEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTIDRAFLTSITTL 370

Query: 368 PDNTTYLGQSFYDSEKELVAKLTTASNGRCSDILGNETFISGKVV-LCFSDIADKTAANM 427
            DN TYLGQ+F   +K++V KL      RC+  LG++    G VV LCF D+A K AA+ 
Sbjct: 371 GDNATYLGQTFL--KKDVVGKLVVMDR-RCAGHLGSDIPTGGNVVLLCFIDLAMKAAASN 430

Query: 428 AAAAAVKANGSGIIVAGQQDDDLFACKQPFPCILVDNTVGTKLLFHVLEGSNPMVRLGRA 487
                 +A   G+I AGQ  D L  C    PCI VD  VGTKLL ++L     ++RL   
Sbjct: 431 PVMPVKQAKVVGVIYAGQHTDILGPC--DVPCIHVDTHVGTKLLTYILSDEKALIRLKAT 490

Query: 488 KTIIGKPISTRIVYFSSRGPNSASPAILKRDIAAPGSNILAAISPHHSSNDKGFGMMSGT 547
           +TI+GKPIS+RI YFSSRGPNS SP ILK DIAAPGSNI+AA+ P+H   DKGF  MSGT
Sbjct: 491 RTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKGGDKGFAAMSGT 550

Query: 548 SMAAPHISGIVALLRSLRPTWSPAAIKSALITTARAKSPSGLPIFANGSPPKAADPFDHG 607
           SMA PHISGIVAL++SLRPTWSPAAIKSALITTAR + PSGLPIFA GSPPK ADPFD+G
Sbjct: 551 SMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGSPPKVADPFDYG 610

Query: 608 GGVVDPNAAIDPGLIYDLDTIEYI-YYLCGMGYKGSDISQLTQQKTECPFPRPSVLDLNL 667
           GGVVD NAA+DPGLIYDL   +YI YY+C MGY  ++IS L+QQK  CP  R SVLDLNL
Sbjct: 611 GGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRASVLDLNL 670

Query: 668 PTITVPELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVLAFNSKVRKISFKV 727
           PTITVP L NSTTVTRTVTNVGN+T++Y+A+I+APPGSKV V P+VL FNS V+KISFKV
Sbjct: 671 PTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNVKKISFKV 730

Query: 728 TFSTALQNNYGYSFGSLTWTDGVHLVKTPLTVRIDFF 762
            FS+ LQ NYGYSFGSLTWTDGVHLVK+PL+VR DFF
Sbjct: 731 KFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDFF 757

BLAST of Spg029776 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 782.7 bits (2020), Expect = 2.6e-226
Identity = 406/759 (53.49%), Postives = 527/759 (69.43%), Query Frame = 0

Query: 14  TIYALFSMFAYKPMAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDLLATVLGSKEKSSE 73
           ++  + S+     +A A  ++ KVHIVYLGE+QHDD K  T+SHH +L+++LGSK+ + E
Sbjct: 7   SVLVVLSLIIVLNVARASAKS-KVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHE 66

Query: 74  SMVYSYRHGFSGFAAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRSWDFLGLSSSPSES 133
           SMVYSYRHGFSGFAAKLT+SQA+K+A+ PEVI V P S Y+L TTR WD+LG   S   S
Sbjct: 67  SMVYSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYELATTRIWDYLG--PSADNS 126

Query: 134 SNLLHRAKMGDNVIIGVVDSGYWPESESFGDKGLGPVPSRWKGTCESGEEFNSTNCNRKV 193
            NL+    MGD  IIGV+D+G WPESESF D G+GPVPS WKG CE GE F STNCNRK+
Sbjct: 127 KNLVSDTNMGDQTIIGVIDTGVWPESESFNDYGVGPVPSHWKGGCEPGENFISTNCNRKL 186

Query: 194 IGARWYMKGFVADNGREAL-ADEYLSPRDINGHGTHTASTAAGSFVTNVSYHGLGVGTLR 253
           IGA++++ GF+A+N   A  + +Y+S RD +GHGTH AS A GSFV NVSY GLG GTLR
Sbjct: 187 IGAKYFINGFLAENQFNATESPDYISARDFDGHGTHVASIAGGSFVPNVSYKGLGRGTLR 246

Query: 254 GGAPLARLAIYKALW----TSDCVGSAADILKAIDEAIHDGVDVLSISIGRFIPLFPEFN 313
           GGAP AR+A+YKA W          S +DI+KAIDEAIHDGVDVLSIS+G  +PL  E +
Sbjct: 247 GGAPRARIAMYKACWYINELDGVTCSFSDIMKAIDEAIHDGVDVLSISLGGRVPLNSETD 306

Query: 314 EENEIAIGSFHAVARGISVVCAGGNDGPLQQTVQNTAPWILTVAASTMDRAFLSSITLPD 373
             + IA G+FHAVA+GI VVCAGGN GP  QTV NTAPWILTVAA+T+DR+F + I L +
Sbjct: 307 LRDGIATGAFHAVAKGIVVVCAGGNAGPSSQTVVNTAPWILTVAATTLDRSFATPIILGN 366

Query: 374 NTTYLGQSFYDSEK---------ELVAKLTTASNGRCSDI-LGNETFISGKVVLCFSDIA 433
           N   LGQ+ Y   +         E         +G C  + L +   ++GKVVLCF+   
Sbjct: 367 NQVILGQAMYIGPELGFTSLVYPEDPGNSIDTFSGVCESLNLNSNRTMAGKVVLCFTTAR 426

Query: 434 DKTAANMAAAAAVKANGSGIIVAGQQDDDLFACKQPFPCILVDNTVGTKLLFHVLEGSNP 493
           D T  + AA+    A G G+I+A     +L  C   FPC+ +DN +GT +LF++    +P
Sbjct: 427 DFTVVSTAASIVKAAGGLGLIIARNPGYNLAPCSDDFPCVAIDNELGTDILFYIRYTGSP 486

Query: 494 MVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKRDIAAPGSNILAAISPHHSSNDKG 553
           +V++  ++T++G+P+ T++  FSSRGPNS SPAILK DIAAPG +ILAA SP+ + N  G
Sbjct: 487 VVKIQPSRTLVGEPVGTKVATFSSRGPNSISPAILKPDIAAPGVSILAATSPNDTLNAGG 546

Query: 554 FGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITTARAKSPSGLPIFANGSPPKA 613
           F M SGTSMAAP ISG++ALL+SL P WSPAA +SA++TTA    P G  I A  S  K 
Sbjct: 547 FVMRSGTSMAAPVISGVIALLKSLHPDWSPAAFRSAIVTTAWRTDPFGEQIAAESSSLKV 606

Query: 614 ADPFDHGGGVVDPNAAIDPGLIYDLDTIEYIYYLCGMGYKGSDISQLTQQKTECPFPRPS 673
            DPFD+GGG+V+P  A +PGLI D+D+ +Y+ YLC  GY  S IS+L  + T C  P+PS
Sbjct: 607 PDPFDYGGGLVNPEKAAEPGLILDMDSQDYVLYLCSAGYNDSSISRLVGKVTVCSNPKPS 666

Query: 674 VLDLNLPTITVPELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVLAFNSKVR 733
           VLD+NLP+IT+P L++  T+TRTVTNVG V S+Y+ ++E P G +V V P+ L FNSK +
Sbjct: 667 VLDINLPSITIPNLKDEVTLTRTVTNVGPVDSVYKVLVEPPLGIQVVVTPETLVFNSKTK 726

Query: 734 KISFKVTFSTALQNNYGYSFGSLTWTDGVHLVKTPLTVR 758
            +SF V  ST  + N G+ FGSLTWTD +H V  P++VR
Sbjct: 727 SVSFTVIVSTTHKINTGFYFGSLTWTDSIHNVVIPVSVR 762

BLAST of Spg029776 vs. TAIR 10
Match: AT1G32940.1 (Subtilase family protein )

HSP 1 Score: 778.1 bits (2008), Expect = 6.5e-225
Identity = 397/747 (53.15%), Postives = 527/747 (70.55%), Query Frame = 0

Query: 27  MAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDLLATVLGSKEKSSESMVYSYRHGFSGF 86
           +  A D++ KVHIVYLGE+QHDD +  ++SHH +L+++LGSK  + ESMVYSYRHGFSGF
Sbjct: 20  VVRASDES-KVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAHESMVYSYRHGFSGF 79

Query: 87  AAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRSWDFLGLSSSPSESSNLLHRAKMGDNV 146
           AAKLT SQA+KLA+ PEV+ V   S Y+L TTR+WD+LGL  S +  +NLL+   MGD V
Sbjct: 80  AAKLTESQAKKLADSPEVVHVMADSFYELATTRTWDYLGL--SVANPNNLLNDTNMGDQV 139

Query: 147 IIGVVDSGYWPESESFGDKGLGPVPSRWKGTCESGEEFNSTNCNRKVIGARWYMKGFVAD 206
           IIG +D+G WPESESF D G+GP+PS WKG CESGE+F STNCNRK+IGA++++ GF+A+
Sbjct: 140 IIGFIDTGVWPESESFNDNGVGPIPSHWKGGCESGEKFISTNCNRKLIGAKYFINGFLAE 199

Query: 207 NG--REALADEYLSPRDINGHGTHTASTAAGSFVTNVSYHGLGVGTLRGGAPLARLAIYK 266
           N       + +Y+S RD  GHGTHTAS A GSFV N+SY GL  G LRGGAP AR+AIYK
Sbjct: 200 NEGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISYKGLAGGNLRGGAPRARIAIYK 259

Query: 267 ALWTSDCVG----SAADILKAIDEAIHDGVDVLSISIGRFIPLFPEFNEENEIAIGSFHA 326
           A W  D +G    S++DILKA+DE++HDGVDVLS+S+G  IPL+PE +  + IA G+FHA
Sbjct: 260 ACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPETDLRDRIATGAFHA 319

Query: 327 VARGISVVCAGGNDGPLQQTVQNTAPWILTVAASTMDRAFLSSITLPDNTTYLGQSFYDS 386
           VA+GI VVCAGGN GP  QTV NTAPWI+TVAA+T+DR+F + ITL +    LGQ+ Y  
Sbjct: 320 VAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPITLGNRKVILGQALYTG 379

Query: 387 EKELVAKLTTASN---------GRCSDI-LGNETFISGKVVLCFSDIADKTAANMAAAAA 446
           ++     L    N         G C  + L     ++GKVVLCF+     TA + AA+  
Sbjct: 380 QELGFTSLVYPENAGFTNETFSGVCERLNLNPNRTMAGKVVLCFTTNTLFTAVSRAASYV 439

Query: 447 VKANGSGIIVAGQQDDDLFACKQPFPCILVDNTVGTKLLFHVLEGSNPMVRLGRAKTIIG 506
             A G G+I+A     +L  C+  FPC+ +D  +GT +L ++    +P+V++  ++T++G
Sbjct: 440 KAAGGLGVIIARNPGYNLTPCRDDFPCVAIDYELGTDVLLYIRSTRSPVVKIQPSRTLVG 499

Query: 507 KPISTRIVYFSSRGPNSASPAILKRDIAAPGSNILAAISPHHSSNDKGFGMMSGTSMAAP 566
           +P+ T++  FSSRGPNS SPAILK DI APG +ILAA SP  +S+  GF +++GTSMAAP
Sbjct: 500 QPVGTKVATFSSRGPNSISPAILKPDIGAPGVSILAATSPDSNSSVGGFDILAGTSMAAP 559

Query: 567 HISGIVALLRSLRPTWSPAAIKSALITTARAKSPSGLPIFANGSPPKAADPFDHGGGVVD 626
            ++G+VALL++L P WSPAA +SA++TTA    P G  IFA GS  K ADPFD+GGG+V+
Sbjct: 560 VVAGVVALLKALHPNWSPAAFRSAIVTTAWRTDPFGEQIFAEGSSRKVADPFDYGGGIVN 619

Query: 627 PNAAIDPGLIYDLDTIEYIYYLCGMGYKGSDISQLTQQKTECPFPRPSVLDLNLPTITVP 686
           P  A DPGLIYD+   +YI YLC  GY  S I+QL    T C  P+ SVLD+NLP+IT+P
Sbjct: 620 PEKAADPGLIYDMGPRDYILYLCSAGYNDSSITQLVGNVTVCSTPKTSVLDVNLPSITIP 679

Query: 687 ELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVLAFNSKVRKISFKVTFSTAL 746
           +L++  T+TRTVTNVG V S+Y+ ++E P G +V V P+ L FNSK + +SF V  ST  
Sbjct: 680 DLKDEVTLTRTVTNVGTVDSVYKVVVEPPLGIQVVVAPETLVFNSKTKNVSFTVRVSTTH 739

Query: 747 QNNYGYSFGSLTWTDGVHLVKTPLTVR 758
           + N G+ FG+L WTD +H V  P++VR
Sbjct: 740 KINTGFYFGNLIWTDSMHNVTIPVSVR 763

BLAST of Spg029776 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 775.4 bits (2001), Expect = 4.2e-224
Identity = 394/766 (51.44%), Postives = 533/766 (69.58%), Query Frame = 0

Query: 14  TIYALFSMFAY----KPMAEADDQNRKVHIVYLGERQHDDTKLTTQSHHDLLATVLGSKE 73
           +IY + S+  +    +         RKVHIVYLGE+QHDD +  T+SHH +L ++LGSKE
Sbjct: 7   SIYVVLSLVIFLNVQRSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKE 66

Query: 74  KSSESMVYSYRHGFSGFAAKLTRSQAQKLAEIPEVIRVFPSSLYKLHTTRSWDFLGLSSS 133
            +++SMVYSYRHGFSGFAAKLT SQA+K+A++P+V+ V P S YKL TTR+WD+LGLS++
Sbjct: 67  DANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAA 126

Query: 134 PSESSNLLHRAKMGDNVIIGVVDSGYWPESESFGDKGLGPVPSRWKGTCESGEEFNSTNC 193
             +S  LLH   MG+ +IIGV+D+G WPESE F D G GPVPS WKG CE+GE FNS+NC
Sbjct: 127 NPKS--LLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNC 186

Query: 194 NRKVIGARWYMKGFVADNG--REALADEYLSPRDINGHGTHTASTAAGSFVTNVSYHGLG 253
           N+K+IGA++++ GF+A+N       + +++SPRD++GHGTH ++ A GSFV N+SY GL 
Sbjct: 187 NKKLIGAKYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLA 246

Query: 254 VGTLRGGAPLARLAIYKALW------TSDCVGSAADILKAIDEAIHDGVDVLSISIGRFI 313
            GT+RGGAP A +A+YKA W      T+ C  S+ADILKA+DEA+HDGVDVLSIS+G  +
Sbjct: 247 GGTVRGGAPRAHIAMYKACWYLDDDDTTTC--SSADILKAMDEAMHDGVDVLSISLGSSV 306

Query: 314 PLFPEFNEENEIAIGSFHAVARGISVVCAGGNDGPLQQTVQNTAPWILTVAASTMDRAFL 373
           PL+ E +  + I  G+FHAV +GI+VVC+GGN GP   TV NTAPWI+TVAA+T+DR+F 
Sbjct: 307 PLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFA 366

Query: 374 SSITLPDNTTYLGQSFYDSEKELVAKLTTASN---------GRCSDILGNET-FISGKVV 433
           + +TL +N   LGQ+ Y         L    N         G C ++L N    + GKVV
Sbjct: 367 TPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVV 426

Query: 434 LCFSDIADKTAANMAAAAAVKANGSGIIVAGQQDDDLFACKQPFPCILVDNTVGTKLLFH 493
           LCF+      A   AA    +A G G+I+A      +  C   FPC+ VD  +GT +L +
Sbjct: 427 LCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLY 486

Query: 494 VLEGSNPMVRLGRAKTIIGKPISTRIVYFSSRGPNSASPAILKRDIAAPGSNILAAISPH 553
                +P+V++  +KT++G+P+ T++  FSSRGPNS +PAILK DIAAPG +ILAA + +
Sbjct: 487 TRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAA-TTN 546

Query: 554 HSSNDKGFGMMSGTSMAAPHISGIVALLRSLRPTWSPAAIKSALITTARAKSPSGLPIFA 613
            + +D+GF M+SGTSMAAP ISG+ ALL++L   WSPAAI+SA++TTA    P G  IFA
Sbjct: 547 TTFSDQGFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFA 606

Query: 614 NGSPPKAADPFDHGGGVVDPNAAIDPGLIYDLDTIEYIYYLCGMGYKGSDISQLTQQKTE 673
            GSPPK ADPFD+GGG+V+P  + +PGL+YD+   +Y+ Y+C +GY  + ISQL  + T 
Sbjct: 607 EGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIGKTTV 666

Query: 674 CPFPRPSVLDLNLPTITVPELRNSTTVTRTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVL 733
           C  P+PSVLD NLP+IT+P L++  T+TRTVTNVG + S+YR  +E P G +VTV P+ L
Sbjct: 667 CSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETL 726

Query: 734 AFNSKVRKISFKVTFSTALQNNYGYSFGSLTWTDGVHLVKTPLTVR 758
            FNS  +K+ FKV  ST  + N GY FGSLTW+D +H V  PL+VR
Sbjct: 727 VFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVR 767

BLAST of Spg029776 vs. TAIR 10
Match: AT4G10520.1 (Subtilase family protein )

HSP 1 Score: 775.0 bits (2000), Expect = 5.5e-224
Identity = 400/729 (54.87%), Postives = 521/729 (71.47%), Query Frame = 0

Query: 36  KVHIVYLGERQHDDTKLTTQSHHDLLATVLGSKEKSSESMVYSYRHGFSGFAAKLTRSQA 95
           KV++VYLGE++HD+ +  T+SHH +L ++LGSKE   +S+VYSYRHGFSGFAAKLT SQA
Sbjct: 28  KVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKLTESQA 87

Query: 96  QKLAEIPEVIRVFPSSLYKLHTTRSWDFLGLSSSPSESSNLLHRAKMGDNVIIGVVDSGY 155
           Q+++E+PEV++V P++LY++ TTR+WD+LG+  SP  S +LL +A MG NVI+GV+DSG 
Sbjct: 88  QQISELPEVVQVIPNTLYEMTTTRTWDYLGV--SPGNSDSLLQKANMGYNVIVGVIDSGV 147

Query: 156 WPESESFGDKGLGPVPSRWKGTCESGEEFN-STNCNRKVIGARWYMKGFVADNG--REAL 215
           WPESE F DKG GP+PSRWKG CESGE FN S +CNRK+IGA++++ G VA+ G      
Sbjct: 148 WPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFGVVNRTQ 207

Query: 216 ADEYLSPRDINGHGTHTASTAAGSFVTNVSYHGLGVGTLRGGAPLARLAIYKALWTSDCV 275
             EYLSPRD  GHGTH AST  GSF+ NVSY GLG GT RGGAP   +A+YKA W+  C 
Sbjct: 208 NPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKACWSGYC- 267

Query: 276 GSAADILKAIDEAIHDGVDVLSISIGRFIPLFPEFNEENEIAIGSFHAVARGISVVCAGG 335
            S AD+LKA+DEAIHDGVD+LS+S+G  +PLFP   E    ++G+FHAVA+GI VV A G
Sbjct: 268 -SGADVLKAMDEAIHDGVDILSLSLGPSVPLFP---ETEHTSVGAFHAVAKGIPVVIAAG 327

Query: 336 NDGPLQQTVQNTAPWILTVAASTMDRAFLSSITLPDNTTYLGQSFYDSEKELVAKLT--- 395
           N GP  QT+ N APW+LTVAA+T DR+F ++ITL +N T LGQ+ Y   +     LT   
Sbjct: 328 NAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIYGGPELGFVGLTYPE 387

Query: 396 TASNGRCSDILGN-ETFISGKVVLCFSDIADKTAANMAAAAAVKANGSGIIVAGQQDDDL 455
           +  +G C  +  N  + + GKVVLCF   A  T +N A AA + A G G+I+A      L
Sbjct: 388 SPLSGDCEKLSANPNSTMEGKVVLCF---AASTPSNAAIAAVINAGGLGLIMAKNPTHSL 447

Query: 456 FACKQPFPCILVDNTVGTKLLFHVLEGSNPMVRLGRAKTIIGKPISTRIVYFSSRGPNSA 515
              ++ FP + +D  +GT +LF++    +P+V++  +KT+ G+ +ST++  FSSRGPNS 
Sbjct: 448 TPTRK-FPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSVSTKVATFSSRGPNSV 507

Query: 516 SPAILKRDIAAPGSNILAAISPHHSSNDKGFGMMSGTSMAAPHISGIVALLRSLRPTWSP 575
           SPAILK DIAAPG NILAAISP+ S ND GF MMSGTSMA P +SG+V LL+SL P WSP
Sbjct: 508 SPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLLKSLHPDWSP 567

Query: 576 AAIKSALITTARAKSPSGLPIFANGSPPKAADPFDHGGGVVDPNAAIDPGLIYDLDTIEY 635
           +AIKSA++TTA    PSG PIFA+GS  K ADPFD+GGG+++P  A+ PGLIYD+ T +Y
Sbjct: 568 SAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLIYDMTTDDY 627

Query: 636 IYYLCGMGYKGSDISQLTQQKTECPFPRPSVLDLNLPTITVPELRNSTTVTRTVTNVGNV 695
           + Y+C + Y    IS++  + T CP P+PSVLDLNLP+IT+P LR   T+TRTVTNVG V
Sbjct: 628 VMYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTLTRTVTNVGPV 687

Query: 696 TSIYRAMIEAPPGSKVTVEPQVLAFNSKVRKISFKVTFSTALQNNYGYSFGSLTWTDGVH 755
            S+Y+ +I+ P G  V V P  L F+    K SF V  ST  + N GY FGSLTWTD +H
Sbjct: 688 NSVYKVVIDPPTGINVAVTPAELVFDYTTTKRSFTVRVSTTHKVNTGYYFGSLTWTDNMH 745

Query: 756 LVKTPLTVR 758
            V  P++VR
Sbjct: 748 NVAIPVSVR 745

BLAST of Spg029776 vs. TAIR 10
Match: AT4G10510.1 (Subtilase family protein )

HSP 1 Score: 774.2 bits (1998), Expect = 9.4e-224
Identity = 393/738 (53.25%), Postives = 524/738 (71.00%), Query Frame = 0

Query: 37  VHIVYLGERQHDDTKLTTQSHHDLLATVLGSKEKSSESMVYSYRHGFSGFAAKLTRSQAQ 96
           VHIVYLGE+QHDD +  T+SHH +L ++LGSKE++  SMV+S+RHGFSGFAAKLT SQA+
Sbjct: 22  VHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEEAHGSMVHSFRHGFSGFAAKLTESQAK 81

Query: 97  KLAEIPEVIRVFPSSLYKLHTTRSWDFLGLSSSPSESSNLLHRAKMGDNVIIGVVDSGYW 156
           K+A++PEV+ V P   YK  TTR+WD+LGL  SP+   NLL++  MG+ +IIG++DSG W
Sbjct: 82  KIADLPEVVHVIPDRFYKPATTRTWDYLGL--SPTNPKNLLNQTNMGEQMIIGIIDSGVW 141

Query: 157 PESESFGDKGLGPVPSRWKGTCESGEEFNSTNCNRKVIGARWYMKGFVAD----NGREAL 216
           PESE F D  +GPVPS WKG CESGE+FNS++CN+K+IGA++++  F+A     N  E+L
Sbjct: 142 PESEVFNDNEIGPVPSHWKGGCESGEDFNSSHCNKKLIGAKYFINAFLATHESFNSSESL 201

Query: 217 ADEYLSPRDINGHGTHTASTAAGSFVTNVSYHGLGVGTLRGGAPLARLAIYKALWTSD-- 276
             +++SPR  NGHGTH A+ A GS+V N SY GL  GT+RGGAP AR+A+YK  W  D  
Sbjct: 202 --DFISPRGYNGHGTHVATIAGGSYVPNTSYKGLAGGTVRGGAPRARIAVYKTCWYLDLD 261

Query: 277 -CVGSAADILKAIDEAIHDGVDVLSISIGRFIPLFPEFNEENEIAIGSFHAVARGISVVC 336
               S+ADILKA+DEAIHDGVDVLS+S+G F PL+PE +  + IA G+FHAV +GI+VVC
Sbjct: 262 IAACSSADILKAMDEAIHDGVDVLSLSLG-FEPLYPETDVRDGIATGAFHAVLKGITVVC 321

Query: 337 AGGNDGPLQQTVQNTAPWILTVAASTMDRAFLSSITLPDNTTYLGQSFYDSEKELVAKLT 396
           A GN GP  QTV NTAPWILTVAA+T+DR+F++ +TL +N   LGQ+ Y   +     L 
Sbjct: 322 AAGNAGPAAQTVGNTAPWILTVAATTLDRSFVTPMTLGNNKVILGQAIYTGTEVGFTSLV 381

Query: 397 TASN---------GRCSDILGNET-FISGKVVLCFSDIADKTAANMAAAAAVKANGSGII 456
              N         G C  +L N    ++GKVVLCF++     +   AA    +A G G+I
Sbjct: 382 YPENPGNSNESFSGTCERLLINSNRTMAGKVVLCFTESPYSISVTRAAHYVKRAGGLGVI 441

Query: 457 VAGQQDDDLFACKQPFPCILVDNTVGTKLLFHVLEGSNPMVRLGRAKTIIGKPISTRIVY 516
           +AGQ  + L  C   FPC+ VD  +GT +LF++    +P+V++  ++T+IG+P+ T++  
Sbjct: 442 IAGQPGNVLRPCLDDFPCVAVDYELGTYILFYIRSNGSPVVKIQPSRTLIGQPVGTKVAS 501

Query: 517 FSSRGPNSASPAILKRDIAAPGSNILAAISPHHSSNDKGFGMMSGTSMAAPHISGIVALL 576
           FSSRGPN  S AILK DIAAPG +ILAA + + + ND+GF  +SGTSMA P ISGIVALL
Sbjct: 502 FSSRGPNPISAAILKPDIAAPGVSILAATTTNTTFNDRGFIFLSGTSMATPTISGIVALL 561

Query: 577 RSLRPTWSPAAIKSALITTARAKSPSGLPIFANGSPPKAADPFDHGGGVVDPNAAIDPGL 636
           ++L P WSPAAI+SA++TTA    P G  IFA GSP K ADPFD+GGG+V+P  A  PGL
Sbjct: 562 KALHPDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKPADPFDYGGGLVNPEKATKPGL 621

Query: 637 IYDLDTIEYIYYLCGMGYKGSDISQLTQQKTECPFPRPSVLDLNLPTITVPELRNSTTVT 696
           +YDL   +Y+ Y+C +GY  + ISQL  + T C +P+PSVLD NLP+IT+P L+   T+ 
Sbjct: 622 VYDLGLEDYVLYMCSVGYNETSISQLVGKGTVCSYPKPSVLDFNLPSITIPNLKEEVTLP 681

Query: 697 RTVTNVGNVTSIYRAMIEAPPGSKVTVEPQVLAFNSKVRKISFKVTFSTALQNNYGYSFG 756
           RT+TNVG + S+YR  +E P G++VTV P+ L FNS  +++SFKV+ ST  + N GY FG
Sbjct: 682 RTLTNVGPLESVYRVAVEPPLGTQVTVTPETLVFNSTTKRVSFKVSVSTTHKINTGYYFG 741

Query: 757 SLTWTDGVHLVKTPLTVR 758
           SLTW+D +H V  PL+VR
Sbjct: 742 SLTWSDSLHNVTIPLSVR 754