Spg029629 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg029629
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionSAP domain-containing protein
Locationscaffold2: 21506897 .. 21508366 (+)
RNA-Seq ExpressionSpg029629
SyntenySpg029629
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CACTTCTCCCTCTTCCCTCCCTCTCTCTCTCTTCGGCTCTGTTTCTTATCCAAACTTCCGCAGCCATTAATGTCCAAATTCCTCCTCTCTCACACCCACCTTCTCACCCTTCCCCACATACACCATTCCTTCTCTCTCAACAATGGCGTTGTCCCCATCCGCTCAGTCCTCTCGGCTCCAGAGAAGCGAGGCAGAAAGAAGCGCCAGTCGCGGCAGCAACAATTACACCAAAAGGACGACGATTCCACTGCGCTCGAGAAGGGCCTTCGCTTCACTTTCATGGAGGAACTCATGGACCGCGCCAGAAACCACGACCCCCTCGGCGTTTCTGATGTCATTTACGATATGGTTGCCGCTGGATTGAGCCCTGGACCTCGCTCGTTCCATGGCTTGGTTGTTTCTCATTCTCTCAATGGTGATACTGAGGGAGCGGTGAGAAGCTCTCTCTCTCTCTCTCTCTGTGCGTTTGTGTGTGGATAATTTTGAATAAATTGACGAGTTCATTTTTGGCGCGCAGTATGTAAATTTGTTGTTTGTGTTGTATTCATAGGCAATGCAGTCAAACTGTTTACATGCTGTTGAAGGTCTAGTGATTGAAATTATCCCCTCTTCCTGCCTTTTCCATTTTCGGATATTTTGAAGTGTAGGATCTTTAAACCTTGTTTTTTGCTTTCCCGTTCGGAGAACTTATCTTATTGGTATCGTAATCGATGTTGAAGTTTTCTCCTCTTATATATCCTTTGCATTTTCACTTAGTAAAGCGAACAGGGAACTCTATTACATAGACTGTATTGAAGTCTCTTGCCCATTTGCATATGTGTGTGTGTGTGTGTGTGAAGGAACAGGATACATTTTTTCCTCTGACAACATAAATCTTGTTTTGGTTCCATTGTAATTTATGTTTTCATTCTTTCTATTCCAGATATGTATTTTTTTAAATTTTATGTTTATGCTGAATGTAGATGCAATCTCTGAGAAGGGAACTAAGTGCTGGACTTCGTCCTCTTCATGAAACTTTTGTTGCATTAGTTCGGTTGTTTGGTTCCAAGGGTCTTGCTTCTAGAGGCTTAGAAATCCTTGCAGCCATGGAGAAATTGAATTATGACATTCGCCAAGCTTGGCTCATTCTTATTGGTAATCGCTTGAACTTCCTGATGAGAATGCTCCCAACAGATACATTTTAATTATGTTTATATTTTGTCTTAGTTGCTTGAGTTATTTTGACTAGAGGAACTTGTAAGGAACAAATATTTAGAAGACGCCAATCAAGTGTTCTTAAAGGGTGCTAAAGGGGGCCTCAGAGCCACGGGCAAGATTTATGATCTTCTAATTGAGGAAGACTGTAAAGCCGGGGATCATTCAAATGCCTTAGAGATCTCATATGAAATGGAGGCTGCTGGGCGGATGGCGACAACCTTTCATTTCAATTGCCTTCTTAGTGTCCAGGTGATGTATCTTACTATTGGATAA

mRNA sequence

ATGTCCAAATTCCTCCTCTCTCACACCCACCTTCTCACCCTTCCCCACATACACCATTCCTTCTCTCTCAACAATGGCGTTGTCCCCATCCGCTCAGTCCTCTCGGCTCCAGAGAAGCGAGGCAGAAAGAAGCGCCAGTCGCGGCAGCAACAATTACACCAAAAGGACGACGATTCCACTGCGCTCGAGAAGGGCCTTCGCTTCACTTTCATGGAGGAACTCATGGACCGCGCCAGAAACCACGACCCCCTCGGCGTTTCTGATGTCATTTACGATATGGTTGCCGCTGGATTGAGCCCTGGACCTCGCTCGTTCCATGGCTTGGTTGTTTCTCATTCTCTCAATGGTGATACTGAGGGAGCGATGCAATCTCTGAGAAGGGAACTAAGTGCTGGACTTCGTCCTCTTCATGAAACTTTTGTTGCATTAGTTCGGTTGTTTGGTTCCAAGGGTCTTGCTTCTAGAGGCTTAGAAATCCTTGCAGCCATGGAGAAATTGAATTATGACATTCGCCAAGCTTGGCTCATTCTTATTGAGGAACTTGTAAGGAACAAATATTTAGAAGACGCCAATCAAGTGTTCTTAAAGGGTGCTAAAGGGGGCCTCAGAGCCACGGGCAAGATTTATGATCTTCTAATTGAGGAAGACTGTAAAGCCGGGGATCATTCAAATGCCTTAGAGATCTCATATGAAATGGAGGCTGCTGGGCGGATGGCGACAACCTTTCATTTCAATTGCCTTCTTAGTGTCCAGGTGATGTATCTTACTATTGGATAA

Coding sequence (CDS)

ATGTCCAAATTCCTCCTCTCTCACACCCACCTTCTCACCCTTCCCCACATACACCATTCCTTCTCTCTCAACAATGGCGTTGTCCCCATCCGCTCAGTCCTCTCGGCTCCAGAGAAGCGAGGCAGAAAGAAGCGCCAGTCGCGGCAGCAACAATTACACCAAAAGGACGACGATTCCACTGCGCTCGAGAAGGGCCTTCGCTTCACTTTCATGGAGGAACTCATGGACCGCGCCAGAAACCACGACCCCCTCGGCGTTTCTGATGTCATTTACGATATGGTTGCCGCTGGATTGAGCCCTGGACCTCGCTCGTTCCATGGCTTGGTTGTTTCTCATTCTCTCAATGGTGATACTGAGGGAGCGATGCAATCTCTGAGAAGGGAACTAAGTGCTGGACTTCGTCCTCTTCATGAAACTTTTGTTGCATTAGTTCGGTTGTTTGGTTCCAAGGGTCTTGCTTCTAGAGGCTTAGAAATCCTTGCAGCCATGGAGAAATTGAATTATGACATTCGCCAAGCTTGGCTCATTCTTATTGAGGAACTTGTAAGGAACAAATATTTAGAAGACGCCAATCAAGTGTTCTTAAAGGGTGCTAAAGGGGGCCTCAGAGCCACGGGCAAGATTTATGATCTTCTAATTGAGGAAGACTGTAAAGCCGGGGATCATTCAAATGCCTTAGAGATCTCATATGAAATGGAGGCTGCTGGGCGGATGGCGACAACCTTTCATTTCAATTGCCTTCTTAGTGTCCAGGTGATGTATCTTACTATTGGATAA

Protein sequence

MSKFLLSHTHLLTLPHIHHSFSLNNGVVPIRSVLSAPEKRGRKKRQSRQQQLHQKDDDSTALEKGLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILAAMEKLNYDIRQAWLILIEELVRNKYLEDANQVFLKGAKGGLRATGKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMATTFHFNCLLSVQVMYLTIG
Homology
BLAST of Spg029629 vs. NCBI nr
Match: XP_038879291.1 (uncharacterized protein LOC120071230 [Benincasa hispida])

HSP 1 Score: 458.0 bits (1177), Expect = 5.4e-125
Identity = 236/252 (93.65%), Postives = 242/252 (96.03%), Query Frame = 0

Query: 1   MSKFLLSHTHLLTLPHIHHSFSLNNGVVPIRSVLSAPEKRGRKKRQSR-QQQLHQKDDDS 60
           MSKFLLSH HLLTLP+ HHSFSLN+GVVPIRSVLSAP+KRGRKKRQ+R QQQLH KD DS
Sbjct: 1   MSKFLLSHAHLLTLPNKHHSFSLNHGVVPIRSVLSAPDKRGRKKRQARQQQQLHSKDHDS 60

Query: 61  TALEKGLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTE 120
           TALEK LRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSH LNGDTE
Sbjct: 61  TALEKSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHVLNGDTE 120

Query: 121 GAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILAAMEKLNYDIRQAWLILIE 180
           GAMQSLRRELSAGL PLHETFVALVRLFGSKGLA+RGLEILAAMEKLNYDIRQAWLIL E
Sbjct: 121 GAMQSLRRELSAGLCPLHETFVALVRLFGSKGLATRGLEILAAMEKLNYDIRQAWLILTE 180

Query: 181 ELVRNKYLEDANQVFLKGAKGGLRATGKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA 240
           ELVRNKYLEDAN+VFLKGAKGGLRAT KIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA
Sbjct: 181 ELVRNKYLEDANKVFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA 240

Query: 241 TTFHFNCLLSVQ 252
           TTFHFNCLLSVQ
Sbjct: 241 TTFHFNCLLSVQ 252

BLAST of Spg029629 vs. NCBI nr
Match: KAG7020856.1 (putative pentatricopeptide repeat-containing protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 456.4 bits (1173), Expect = 1.6e-124
Identity = 237/259 (91.51%), Postives = 246/259 (94.98%), Query Frame = 0

Query: 1   MSKFLLSHTHLLTLPHIHHSFSLNNGVV-PIRSVLSAPEKRGRKKRQSRQQQLHQKDDDS 60
           MSKFLLSH+ LLTLPH HHSFSL+NGV+ PIRSVLS  EKRGRKKRQSRQQQL QKDDDS
Sbjct: 1   MSKFLLSHSCLLTLPHKHHSFSLHNGVLPPIRSVLST-EKRGRKKRQSRQQQLQQKDDDS 60

Query: 61  TALEKGLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTE 120
           T LEK LRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSH LN D E
Sbjct: 61  TVLEKSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHVLNADAE 120

Query: 121 GAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILAAMEKLNYDIRQAWLILIE 180
           GAMQSLR+ELS GLRPLHETFVALVRLFG+KGLA+RGLEILAAMEKLNYDIRQAWLILIE
Sbjct: 121 GAMQSLRKELSTGLRPLHETFVALVRLFGNKGLATRGLEILAAMEKLNYDIRQAWLILIE 180

Query: 181 ELVRNKYLEDANQVFLKGAKGGLRATGKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA 240
           ELV+NKYLEDAN+VFLKGAKGGLRAT KIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA
Sbjct: 181 ELVKNKYLEDANKVFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA 240

Query: 241 TTFHFNCLLSVQVMYLTIG 259
           TTFHFNCLLSVQVMYL+IG
Sbjct: 241 TTFHFNCLLSVQVMYLSIG 258

BLAST of Spg029629 vs. NCBI nr
Match: KAA0038380.1 (Pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa] >TYJ97014.1 Pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 446.4 bits (1147), Expect = 1.6e-121
Identity = 228/252 (90.48%), Postives = 240/252 (95.24%), Query Frame = 0

Query: 1   MSKFLLSHTHLLTLPHIHHSFSLNNGVVPIRSVLSAPEKRGRKKRQSR-QQQLHQKDDDS 60
           MSK LLSH HLLTLP+ H SFSLN+G++PIRSVLSAP+KRGRKKRQSR QQQL  KDDDS
Sbjct: 1   MSKLLLSHAHLLTLPYNHRSFSLNHGLLPIRSVLSAPDKRGRKKRQSRHQQQLQLKDDDS 60

Query: 61  TALEKGLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTE 120
           T+LE  LRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSH+LNGDTE
Sbjct: 61  TSLENSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHTLNGDTE 120

Query: 121 GAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILAAMEKLNYDIRQAWLILIE 180
           GAMQSLRRELS+GLRPLHETFVALVRLFGSKGLA+RGLEILAAME+LNYDIRQAWLIL E
Sbjct: 121 GAMQSLRRELSSGLRPLHETFVALVRLFGSKGLANRGLEILAAMERLNYDIRQAWLILTE 180

Query: 181 ELVRNKYLEDANQVFLKGAKGGLRATGKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA 240
           ELVRNKYLEDAN+VFLKGAK GLRAT KIYDL+IEEDCKAGDHSNALEISYEMEAAGRMA
Sbjct: 181 ELVRNKYLEDANKVFLKGAKAGLRATDKIYDLMIEEDCKAGDHSNALEISYEMEAAGRMA 240

Query: 241 TTFHFNCLLSVQ 252
           TTFHFNCLLSVQ
Sbjct: 241 TTFHFNCLLSVQ 252

BLAST of Spg029629 vs. NCBI nr
Match: XP_008443747.1 (PREDICTED: uncharacterized protein LOC103487261 isoform X2 [Cucumis melo])

HSP 1 Score: 446.4 bits (1147), Expect = 1.6e-121
Identity = 228/252 (90.48%), Postives = 240/252 (95.24%), Query Frame = 0

Query: 1   MSKFLLSHTHLLTLPHIHHSFSLNNGVVPIRSVLSAPEKRGRKKRQSR-QQQLHQKDDDS 60
           MSK LLSH HLLTLP+ H SFSLN+G++PIRSVLSAP+KRGRKKRQSR QQQL  KDDDS
Sbjct: 1   MSKLLLSHAHLLTLPYNHRSFSLNHGLLPIRSVLSAPDKRGRKKRQSRHQQQLQLKDDDS 60

Query: 61  TALEKGLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTE 120
           T+LE  LRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSH+LNGDTE
Sbjct: 61  TSLENSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHTLNGDTE 120

Query: 121 GAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILAAMEKLNYDIRQAWLILIE 180
           GAMQSLRRELS+GLRPLHETFVALVRLFGSKGLA+RGLEILAAME+LNYDIRQAWLIL E
Sbjct: 121 GAMQSLRRELSSGLRPLHETFVALVRLFGSKGLANRGLEILAAMERLNYDIRQAWLILTE 180

Query: 181 ELVRNKYLEDANQVFLKGAKGGLRATGKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA 240
           ELVRNKYLEDAN+VFLKGAK GLRAT KIYDL+IEEDCKAGDHSNALEISYEMEAAGRMA
Sbjct: 181 ELVRNKYLEDANKVFLKGAKAGLRATDKIYDLMIEEDCKAGDHSNALEISYEMEAAGRMA 240

Query: 241 TTFHFNCLLSVQ 252
           TTFHFNCLLSVQ
Sbjct: 241 TTFHFNCLLSVQ 252

BLAST of Spg029629 vs. NCBI nr
Match: XP_008443746.1 (PREDICTED: uncharacterized protein LOC103487261 isoform X1 [Cucumis melo])

HSP 1 Score: 446.4 bits (1147), Expect = 1.6e-121
Identity = 228/252 (90.48%), Postives = 240/252 (95.24%), Query Frame = 0

Query: 1   MSKFLLSHTHLLTLPHIHHSFSLNNGVVPIRSVLSAPEKRGRKKRQSR-QQQLHQKDDDS 60
           MSK LLSH HLLTLP+ H SFSLN+G++PIRSVLSAP+KRGRKKRQSR QQQL  KDDDS
Sbjct: 1   MSKLLLSHAHLLTLPYNHRSFSLNHGLLPIRSVLSAPDKRGRKKRQSRHQQQLQLKDDDS 60

Query: 61  TALEKGLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTE 120
           T+LE  LRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSH+LNGDTE
Sbjct: 61  TSLENSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHTLNGDTE 120

Query: 121 GAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILAAMEKLNYDIRQAWLILIE 180
           GAMQSLRRELS+GLRPLHETFVALVRLFGSKGLA+RGLEILAAME+LNYDIRQAWLIL E
Sbjct: 121 GAMQSLRRELSSGLRPLHETFVALVRLFGSKGLANRGLEILAAMERLNYDIRQAWLILTE 180

Query: 181 ELVRNKYLEDANQVFLKGAKGGLRATGKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA 240
           ELVRNKYLEDAN+VFLKGAK GLRAT KIYDL+IEEDCKAGDHSNALEISYEMEAAGRMA
Sbjct: 181 ELVRNKYLEDANKVFLKGAKAGLRATDKIYDLMIEEDCKAGDHSNALEISYEMEAAGRMA 240

Query: 241 TTFHFNCLLSVQ 252
           TTFHFNCLLSVQ
Sbjct: 241 TTFHFNCLLSVQ 252

BLAST of Spg029629 vs. ExPASy Swiss-Prot
Match: O64624 (Pentatricopeptide repeat-containing protein At2g18940, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g18940 PE=2 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 4.5e-05
Identity = 35/122 (28.69%), Postives = 59/122 (48.36%), Query Frame = 0

Query: 130 SAGLRPLHETFVALVRLFGSKGLASRGLEILAAMEKLNYDIRQ-AWLILIEELVRNKYLE 189
           S G  P   T+ AL+++FG  G+ +  L +L  ME+ +       +  L+   VR  + +
Sbjct: 309 SCGYEPGTVTYNALLQVFGKAGVYTEALSVLKEMEENSCPADSVTYNELVAAYVRAGFSK 368

Query: 190 DANQVFLKGAKGGLRATGKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMATTFHFNCLL 249
           +A  V     K G+      Y  +I+   KAG    AL++ Y M+ AG +  T  +N +L
Sbjct: 369 EAAGVIEMMTKKGVMPNAITYTTVIDAYGKAGKEDEALKLFYSMKEAGCVPNTCTYNAVL 428

Query: 250 SV 251
           S+
Sbjct: 429 SL 430

BLAST of Spg029629 vs. ExPASy Swiss-Prot
Match: Q9FIX3 (Pentatricopeptide repeat-containing protein At5g39710 OS=Arabidopsis thaliana OX=3702 GN=EMB2745 PE=2 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 3.8e-04
Identity = 42/199 (21.11%), Postives = 78/199 (39.20%), Query Frame = 0

Query: 62  LEKGLR---FTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDT 121
           +EKG++    T+   +             D+  +M+  GL P   ++  L+ ++ + GD 
Sbjct: 477 VEKGIKPDTITYSSLIQGFCEQRRTKEACDLYEEMLRVGLPPDEFTYTALINAYCMEGDL 536

Query: 122 EGAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILAAM---EKLNYDIRQAWL 181
           E A+Q     +  G+ P   T+  L+     +        +L  +   E +  D+    L
Sbjct: 537 EKALQLHNEMVEKGVLPDVVTYSVLINGLNKQSRTREAKRLLLKLFYEESVPSDVTYHTL 596

Query: 182 I-------------LIEELVRNKYLEDANQVFLKGAKGGLRATGKIYDLLIEEDCKAGDH 241
           I             LI+       + +A+QVF        +  G  Y+++I   C+AGD 
Sbjct: 597 IENCSNIEFKSVVSLIKGFCMKGMMTEADQVFESMLGKNHKPDGTAYNIMIHGHCRAGDI 656

BLAST of Spg029629 vs. ExPASy Swiss-Prot
Match: O04504 (Pentatricopeptide repeat-containing protein At1g09820 OS=Arabidopsis thaliana OX=3702 GN=At1g09820 PE=2 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 3.8e-04
Identity = 36/162 (22.22%), Postives = 72/162 (44.44%), Query Frame = 0

Query: 89  VIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGAMQSLRRELSAGLRPLHETFVALVRLFG 148
           V+ +MV   +SP   +F+ L+     + +  G+M+  +  L   ++P   ++ +L+    
Sbjct: 283 VLKEMVENDVSPNLTTFNILIDGFWKDDNLPGSMKVFKEMLDQDVKPNVISYNSLINGLC 342

Query: 149 SKGLASRGLEILAAMEKLNYDIRQ-AWLILIEELVRNKYLEDANQVFLKGAKGGLRATGK 208
           + G  S  + +   M           +  LI    +N  L++A  +F      G   T +
Sbjct: 343 NGGKISEAISMRDKMVSAGVQPNLITYNALINGFCKNDMLKEALDMFGSVKGQGAVPTTR 402

Query: 209 IYDLLIEEDCKAGDHSNALEISYEMEAAGRMATTFHFNCLLS 250
           +Y++LI+  CK G   +   +  EME  G +     +NCL++
Sbjct: 403 MYNMLIDAYCKLGKIDDGFALKEEMEREGIVPDVGTYNCLIA 444

BLAST of Spg029629 vs. ExPASy Swiss-Prot
Match: Q0WPZ6 (Pentatricopeptide repeat-containing protein At2g17140 OS=Arabidopsis thaliana OX=3702 GN=At2g17140 PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 8.4e-04
Identity = 39/149 (26.17%), Postives = 66/149 (44.30%), Query Frame = 0

Query: 86  VSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGAMQSLRRELSAGLRPLHETFVALVR 145
           VS +  DMV  G++P   +F+ L+ +   +   + A +        G +P   TF  LVR
Sbjct: 131 VSWLYKDMVLCGIAPQTYTFNLLIRALCDSSCVDAARELFDEMPEKGCKPNEFTFGILVR 190

Query: 146 LFGSKGLASRGLEILAAMEKLN-YDIRQAWLILIEELVRNKYLEDANQVFLKGAKGGLRA 205
            +   GL  +GLE+L AME       +  +  ++    R    +D+ ++  K  + GL  
Sbjct: 191 GYCKAGLTDKGLELLNAMESFGVLPNKVIYNTIVSSFCREGRNDDSEKMVEKMREEGLVP 250

Query: 206 TGKIYDLLIEEDCKAGDHSNALEISYEME 234
               ++  I   CK G   +A  I  +ME
Sbjct: 251 DIVTFNSRISALCKEGKVLDASRIFSDME 279

BLAST of Spg029629 vs. ExPASy TrEMBL
Match: A0A5A7T4U0 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold506G00670 PE=4 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 7.9e-122
Identity = 228/252 (90.48%), Postives = 240/252 (95.24%), Query Frame = 0

Query: 1   MSKFLLSHTHLLTLPHIHHSFSLNNGVVPIRSVLSAPEKRGRKKRQSR-QQQLHQKDDDS 60
           MSK LLSH HLLTLP+ H SFSLN+G++PIRSVLSAP+KRGRKKRQSR QQQL  KDDDS
Sbjct: 1   MSKLLLSHAHLLTLPYNHRSFSLNHGLLPIRSVLSAPDKRGRKKRQSRHQQQLQLKDDDS 60

Query: 61  TALEKGLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTE 120
           T+LE  LRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSH+LNGDTE
Sbjct: 61  TSLENSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHTLNGDTE 120

Query: 121 GAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILAAMEKLNYDIRQAWLILIE 180
           GAMQSLRRELS+GLRPLHETFVALVRLFGSKGLA+RGLEILAAME+LNYDIRQAWLIL E
Sbjct: 121 GAMQSLRRELSSGLRPLHETFVALVRLFGSKGLANRGLEILAAMERLNYDIRQAWLILTE 180

Query: 181 ELVRNKYLEDANQVFLKGAKGGLRATGKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA 240
           ELVRNKYLEDAN+VFLKGAK GLRAT KIYDL+IEEDCKAGDHSNALEISYEMEAAGRMA
Sbjct: 181 ELVRNKYLEDANKVFLKGAKAGLRATDKIYDLMIEEDCKAGDHSNALEISYEMEAAGRMA 240

Query: 241 TTFHFNCLLSVQ 252
           TTFHFNCLLSVQ
Sbjct: 241 TTFHFNCLLSVQ 252

BLAST of Spg029629 vs. ExPASy TrEMBL
Match: A0A1S3B8T6 (uncharacterized protein LOC103487261 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487261 PE=4 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 7.9e-122
Identity = 228/252 (90.48%), Postives = 240/252 (95.24%), Query Frame = 0

Query: 1   MSKFLLSHTHLLTLPHIHHSFSLNNGVVPIRSVLSAPEKRGRKKRQSR-QQQLHQKDDDS 60
           MSK LLSH HLLTLP+ H SFSLN+G++PIRSVLSAP+KRGRKKRQSR QQQL  KDDDS
Sbjct: 1   MSKLLLSHAHLLTLPYNHRSFSLNHGLLPIRSVLSAPDKRGRKKRQSRHQQQLQLKDDDS 60

Query: 61  TALEKGLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTE 120
           T+LE  LRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSH+LNGDTE
Sbjct: 61  TSLENSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHTLNGDTE 120

Query: 121 GAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILAAMEKLNYDIRQAWLILIE 180
           GAMQSLRRELS+GLRPLHETFVALVRLFGSKGLA+RGLEILAAME+LNYDIRQAWLIL E
Sbjct: 121 GAMQSLRRELSSGLRPLHETFVALVRLFGSKGLANRGLEILAAMERLNYDIRQAWLILTE 180

Query: 181 ELVRNKYLEDANQVFLKGAKGGLRATGKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA 240
           ELVRNKYLEDAN+VFLKGAK GLRAT KIYDL+IEEDCKAGDHSNALEISYEMEAAGRMA
Sbjct: 181 ELVRNKYLEDANKVFLKGAKAGLRATDKIYDLMIEEDCKAGDHSNALEISYEMEAAGRMA 240

Query: 241 TTFHFNCLLSVQ 252
           TTFHFNCLLSVQ
Sbjct: 241 TTFHFNCLLSVQ 252

BLAST of Spg029629 vs. ExPASy TrEMBL
Match: A0A1S3B9H7 (uncharacterized protein LOC103487261 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103487261 PE=4 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 7.9e-122
Identity = 228/252 (90.48%), Postives = 240/252 (95.24%), Query Frame = 0

Query: 1   MSKFLLSHTHLLTLPHIHHSFSLNNGVVPIRSVLSAPEKRGRKKRQSR-QQQLHQKDDDS 60
           MSK LLSH HLLTLP+ H SFSLN+G++PIRSVLSAP+KRGRKKRQSR QQQL  KDDDS
Sbjct: 1   MSKLLLSHAHLLTLPYNHRSFSLNHGLLPIRSVLSAPDKRGRKKRQSRHQQQLQLKDDDS 60

Query: 61  TALEKGLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTE 120
           T+LE  LRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSH+LNGDTE
Sbjct: 61  TSLENSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHTLNGDTE 120

Query: 121 GAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILAAMEKLNYDIRQAWLILIE 180
           GAMQSLRRELS+GLRPLHETFVALVRLFGSKGLA+RGLEILAAME+LNYDIRQAWLIL E
Sbjct: 121 GAMQSLRRELSSGLRPLHETFVALVRLFGSKGLANRGLEILAAMERLNYDIRQAWLILTE 180

Query: 181 ELVRNKYLEDANQVFLKGAKGGLRATGKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA 240
           ELVRNKYLEDAN+VFLKGAK GLRAT KIYDL+IEEDCKAGDHSNALEISYEMEAAGRMA
Sbjct: 181 ELVRNKYLEDANKVFLKGAKAGLRATDKIYDLMIEEDCKAGDHSNALEISYEMEAAGRMA 240

Query: 241 TTFHFNCLLSVQ 252
           TTFHFNCLLSVQ
Sbjct: 241 TTFHFNCLLSVQ 252

BLAST of Spg029629 vs. ExPASy TrEMBL
Match: A0A6J1EQ88 (uncharacterized protein LOC111436825 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436825 PE=4 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 3.0e-121
Identity = 230/252 (91.27%), Postives = 238/252 (94.44%), Query Frame = 0

Query: 1   MSKFLLSHTHLLTLPHIHHSFSLNNGVV-PIRSVLSAPEKRGRKKRQSRQQQLHQKDDDS 60
           MSKFLLSH++LLTLPH HHSFSL+NGV  PIRSVLS  EKRGRKKRQSRQQQL QKDDDS
Sbjct: 1   MSKFLLSHSYLLTLPHKHHSFSLHNGVFPPIRSVLST-EKRGRKKRQSRQQQLQQKDDDS 60

Query: 61  TALEKGLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTE 120
           T  EK LRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSH LN D E
Sbjct: 61  TVFEKSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHVLNADAE 120

Query: 121 GAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILAAMEKLNYDIRQAWLILIE 180
           GAMQSLR+ELS GLRPLHETFVALVRLFG+KGLA+RGLEILAAMEKLNYDIRQAWLILIE
Sbjct: 121 GAMQSLRKELSTGLRPLHETFVALVRLFGNKGLATRGLEILAAMEKLNYDIRQAWLILIE 180

Query: 181 ELVRNKYLEDANQVFLKGAKGGLRATGKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA 240
           ELV+NKYLEDAN+VFLKGAKGGLRAT KIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA
Sbjct: 181 ELVKNKYLEDANKVFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA 240

Query: 241 TTFHFNCLLSVQ 252
           TTFHFNCLLSVQ
Sbjct: 241 TTFHFNCLLSVQ 251

BLAST of Spg029629 vs. ExPASy TrEMBL
Match: A0A6J1L2D9 (uncharacterized protein LOC111499221 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499221 PE=4 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 1.3e-119
Identity = 229/252 (90.87%), Postives = 237/252 (94.05%), Query Frame = 0

Query: 1   MSKFLLSHTHLLTLPHIHHSFSLNNGVV-PIRSVLSAPEKRGRKKRQSRQQQLHQKDDDS 60
           MSKFLLSH+ LLTLPH HHSFSL+N V+ PIRSVLS  EKRGRKKRQSRQQQL QKD DS
Sbjct: 1   MSKFLLSHSCLLTLPHKHHSFSLHNAVLPPIRSVLST-EKRGRKKRQSRQQQLQQKDYDS 60

Query: 61  TALEKGLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTE 120
           T LEK LRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSH LN D E
Sbjct: 61  TVLEKSLRFTFMEELMDRARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHVLNADAE 120

Query: 121 GAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGLEILAAMEKLNYDIRQAWLILIE 180
           GAMQSLR+ELS GLRPLHETFVALVRLFG+KGLA+RGLEILAAMEKLNYDIRQAWLILIE
Sbjct: 121 GAMQSLRKELSTGLRPLHETFVALVRLFGNKGLATRGLEILAAMEKLNYDIRQAWLILIE 180

Query: 181 ELVRNKYLEDANQVFLKGAKGGLRATGKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA 240
           ELV+NKYLEDAN+VFLKGAKGGLRAT KIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA
Sbjct: 181 ELVKNKYLEDANKVFLKGAKGGLRATDKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMA 240

Query: 241 TTFHFNCLLSVQ 252
           TTFHFNCLLSVQ
Sbjct: 241 TTFHFNCLLSVQ 251

BLAST of Spg029629 vs. TAIR 10
Match: AT3G04260.1 (plastid transcriptionally active 3 )

HSP 1 Score: 326.2 bits (835), Expect = 2.3e-89
Identity = 165/234 (70.51%), Postives = 196/234 (83.76%), Query Frame = 0

Query: 26  GVVPIRSVLSAPEKRGRKKRQSRQQQLHQKDDD--------STALEKGLRFTFMEELMDR 85
           G+  IR  +SAPEK+ R++R+ ++    + DD          +ALE+ LR TFM+ELM+R
Sbjct: 24  GISSIRCSISAPEKKPRRRRKQKRGDGAENDDSLSFGSGEAVSALERSLRLTFMDELMER 83

Query: 86  ARNHDPLGVSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGAMQSLRRELSAGLRPLH 145
           ARN D  GVS+VIYDM+AAGLSPGPRSFHGLVV+H+LNGD +GAM SLR+EL AG RPL 
Sbjct: 84  ARNRDTSGVSEVIYDMIAAGLSPGPRSFHGLVVAHALNGDEQGAMHSLRKELGAGQRPLP 143

Query: 146 ETFVALVRLFGSKGLASRGLEILAAMEKLNYDIRQAWLILIEELVRNKYLEDANQVFLKG 205
           ET +ALVRL GSKG A+RGLEILAAMEKL YDIRQAWLIL+EEL+R  +LEDAN+VFLKG
Sbjct: 144 ETMIALVRLSGSKGNATRGLEILAAMEKLKYDIRQAWLILVEELMRINHLEDANKVFLKG 203

Query: 206 AKGGLRATGKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMATTFHFNCLLSVQ 252
           A+GG+RAT ++YDL+IEEDCKAGDHSNAL+ISYEMEAAGRMATTFHFNCLLSVQ
Sbjct: 204 ARGGMRATDQLYDLMIEEDCKAGDHSNALDISYEMEAAGRMATTFHFNCLLSVQ 257

BLAST of Spg029629 vs. TAIR 10
Match: AT2G18940.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 50.1 bits (118), Expect = 3.2e-06
Identity = 35/122 (28.69%), Postives = 59/122 (48.36%), Query Frame = 0

Query: 130 SAGLRPLHETFVALVRLFGSKGLASRGLEILAAMEKLNYDIRQ-AWLILIEELVRNKYLE 189
           S G  P   T+ AL+++FG  G+ +  L +L  ME+ +       +  L+   VR  + +
Sbjct: 309 SCGYEPGTVTYNALLQVFGKAGVYTEALSVLKEMEENSCPADSVTYNELVAAYVRAGFSK 368

Query: 190 DANQVFLKGAKGGLRATGKIYDLLIEEDCKAGDHSNALEISYEMEAAGRMATTFHFNCLL 249
           +A  V     K G+      Y  +I+   KAG    AL++ Y M+ AG +  T  +N +L
Sbjct: 369 EAAGVIEMMTKKGVMPNAITYTTVIDAYGKAGKEDEALKLFYSMKEAGCVPNTCTYNAVL 428

Query: 250 SV 251
           S+
Sbjct: 429 SL 430

BLAST of Spg029629 vs. TAIR 10
Match: AT1G09820.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 47.0 bits (110), Expect = 2.7e-05
Identity = 36/162 (22.22%), Postives = 72/162 (44.44%), Query Frame = 0

Query: 89  VIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGAMQSLRRELSAGLRPLHETFVALVRLFG 148
           V+ +MV   +SP   +F+ L+     + +  G+M+  +  L   ++P   ++ +L+    
Sbjct: 283 VLKEMVENDVSPNLTTFNILIDGFWKDDNLPGSMKVFKEMLDQDVKPNVISYNSLINGLC 342

Query: 149 SKGLASRGLEILAAMEKLNYDIRQ-AWLILIEELVRNKYLEDANQVFLKGAKGGLRATGK 208
           + G  S  + +   M           +  LI    +N  L++A  +F      G   T +
Sbjct: 343 NGGKISEAISMRDKMVSAGVQPNLITYNALINGFCKNDMLKEALDMFGSVKGQGAVPTTR 402

Query: 209 IYDLLIEEDCKAGDHSNALEISYEMEAAGRMATTFHFNCLLS 250
           +Y++LI+  CK G   +   +  EME  G +     +NCL++
Sbjct: 403 MYNMLIDAYCKLGKIDDGFALKEEMEREGIVPDVGTYNCLIA 444

BLAST of Spg029629 vs. TAIR 10
Match: AT2G17140.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 45.8 bits (107), Expect = 6.0e-05
Identity = 39/149 (26.17%), Postives = 66/149 (44.30%), Query Frame = 0

Query: 86  VSDVIYDMVAAGLSPGPRSFHGLVVSHSLNGDTEGAMQSLRRELSAGLRPLHETFVALVR 145
           VS +  DMV  G++P   +F+ L+ +   +   + A +        G +P   TF  LVR
Sbjct: 131 VSWLYKDMVLCGIAPQTYTFNLLIRALCDSSCVDAARELFDEMPEKGCKPNEFTFGILVR 190

Query: 146 LFGSKGLASRGLEILAAMEKLN-YDIRQAWLILIEELVRNKYLEDANQVFLKGAKGGLRA 205
            +   GL  +GLE+L AME       +  +  ++    R    +D+ ++  K  + GL  
Sbjct: 191 GYCKAGLTDKGLELLNAMESFGVLPNKVIYNTIVSSFCREGRNDDSEKMVEKMREEGLVP 250

Query: 206 TGKIYDLLIEEDCKAGDHSNALEISYEME 234
               ++  I   CK G   +A  I  +ME
Sbjct: 251 DIVTFNSRISALCKEGKVLDASRIFSDME 279

BLAST of Spg029629 vs. TAIR 10
Match: AT1G79540.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 1.3e-04
Identity = 50/206 (24.27%), Postives = 92/206 (44.66%), Query Frame = 0

Query: 39  KRGRKKRQSRQQQLHQKDDDSTALEKGLR-FTFMEELMDRARNHDPLGVSDVIYDMVAAG 98
           K GR        +L +KD        GLR ++ + + + RAR +      ++  +M+   
Sbjct: 280 KLGRMVEAFELLRLFEKD----GFVLGLRGYSSLIDGLFRARRYTQ--AFELYANMLKKN 339

Query: 99  LSPGPRSFHGLVVSHSLNGDTEGAMQSLRRELSAGLRPLHETFVALVRLFGSKGLASRGL 158
           + P    +  L+   S  G  E A++ L    S G+ P    + A+++    +GL   G 
Sbjct: 340 IKPDIILYTILIQGLSKAGKIEDALKLLSSMPSKGISPDTYCYNAVIKALCGRGLLEEGR 399

Query: 159 EI-LAAMEKLNYDIRQAWLILIEELVRNKYLEDANQVFLKGAKGGLRATGKIYDLLIEED 218
            + L   E  ++       ILI  + RN  + +A ++F +  K G   +   ++ LI+  
Sbjct: 400 SLQLEMSETESFPDACTHTILICSMCRNGLVREAEEIFTEIEKSGCSPSVATFNALIDGL 459

Query: 219 CKAGDHSNALEISYEMEAAGRMATTF 243
           CK+G+   A  + ++ME  GR A+ F
Sbjct: 460 CKSGELKEARLLLHKME-VGRPASLF 478

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879291.15.4e-12593.65uncharacterized protein LOC120071230 [Benincasa hispida][more]
KAG7020856.11.6e-12491.51putative pentatricopeptide repeat-containing protein [Cucurbita argyrosperma sub... [more]
KAA0038380.11.6e-12190.48Pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa] >TYJ97014... [more]
XP_008443747.11.6e-12190.48PREDICTED: uncharacterized protein LOC103487261 isoform X2 [Cucumis melo][more]
XP_008443746.11.6e-12190.48PREDICTED: uncharacterized protein LOC103487261 isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
O646244.5e-0528.69Pentatricopeptide repeat-containing protein At2g18940, chloroplastic OS=Arabidop... [more]
Q9FIX33.8e-0421.11Pentatricopeptide repeat-containing protein At5g39710 OS=Arabidopsis thaliana OX... [more]
O045043.8e-0422.22Pentatricopeptide repeat-containing protein At1g09820 OS=Arabidopsis thaliana OX... [more]
Q0WPZ68.4e-0426.17Pentatricopeptide repeat-containing protein At2g17140 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A5A7T4U07.9e-12290.48Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3B8T67.9e-12290.48uncharacterized protein LOC103487261 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A1S3B9H77.9e-12290.48uncharacterized protein LOC103487261 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1EQ883.0e-12191.27uncharacterized protein LOC111436825 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1L2D91.3e-11990.87uncharacterized protein LOC111499221 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT3G04260.12.3e-8970.51plastid transcriptionally active 3 [more]
AT2G18940.13.2e-0628.69Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G09820.12.7e-0522.22Pentatricopeptide repeat (PPR-like) superfamily protein [more]
AT2G17140.16.0e-0526.17Pentatricopeptide repeat (PPR) superfamily protein [more]
AT1G79540.11.3e-0424.27Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 170..253
e-value: 1.3E-6
score: 29.9
coord: 38..169
e-value: 6.1E-6
score: 27.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 34..59
NoneNo IPR availablePANTHERPTHR31407:SF5PLASTID TRANSCRIPTIONALLY ACTIVE 3coord: 22..252
NoneNo IPR availablePANTHERPTHR31407FAMILY NOT NAMEDcoord: 22..252

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg029629.1Spg029629.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044260 cellular macromolecule metabolic process
biological_process GO:0098869 cellular oxidant detoxification
biological_process GO:0006807 nitrogen compound metabolic process
biological_process GO:0044238 primary metabolic process
biological_process GO:0006979 response to oxidative stress
molecular_function GO:0020037 heme binding
molecular_function GO:0004601 peroxidase activity
molecular_function GO:0005515 protein binding