Spg029487 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg029487
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	4-coumarate--CoA ligase
Location	scaffold12: 31607086 .. 31610477 (-)
RNA-Seq Expression	Spg029487
Synteny	Spg029487

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGCTCGGCATTTGCAAAGGAGCCACCAATGCCAACAAAGCAGGGGCGGATTTATGAAGGGGTCGTGCCCCCCCTCAATTTAAACGTATATATTATATATATGTTTTGCCGATTAAATTTTAAAAGTTACGTTTGACATGTTGGTTTCTGTCCCACTGTGTGTGATGGTGTCCCCCTCTCCGGTCTTGGGTTCTAGCCAAATGCACGCTATATTTTATTTTTTTCTGTTATTTTCAGTTTTTCTTTAATTTAAACATTTTTAAAATATATTTTTATGTTTTTGCTTAAGCTTTTTATCACTATCTATTAAATAAAATTTTCATTTTCTTTAATGAATTTAAATCCACCTATGAAATAGAACGAATATTAAAATATTATTGTTGATCTTTGTACTTTGTGACTCATTCTAATTTAGTTCATATATTTTCATATATTCAAATTTAGCCAATGTAATTTCAATAAATCTTTAGATTAGTCATTATTAATTTGAAGTTAGTCTAAAACATGGTAATAATCATAATTTTCACTCAAGAAAACAAACCAATTGTTAATATGTTTTCATAATTTATAAAGCGAAAACTAATGGAGACTAAATTTGTTTTGAAAAAGTTAGCCATAGTCTAATTCTAAGATTATTAAAAGTACAATGATTATATTTGGACATTGAAATTATCGACAATAAAATAAAATAAGTTCCAAAGTACAATGACCAAAAGAATATGAAATGAGTTTATAAGTTTCTTTTTAAAAAATTAGGAAAAAAAATCAATTCTACCCCAAAACTTTGGGGTCAATTAAAACTCAATTTAAATCCTAAACATTCATAATTGAATTTTATGTCTAATGAAACTATAAACTTTCAATTGTGTGTTTAGTAAGCCTATGAATTTTGAAAAATGTCAAATAACACCTATCAGACACAAAATTGAAAATTCAAGAACTTATTGGACACAAAATTAAAAGTTCAGGATTCTCTTAGACACATTTTTAAGTTTAGGAACCATAGACACAAATATGAAAGTTCAGAGACTAAACTTGTAATTTAACCTAATCGATATTAGGTATTTAAATATTATATCAATGCTTCTAAAGTTGCATTTATATGAACTTGTGCCCCCGGTATAAAATTCTGGATCCGCCACTGCTCACACTTTATACCCAAATATTAATATTATATCATTTTTCACTTTTATATTTAATTTTTCAATTAACTCTATAAATTATGGTATATACAGGTGGCACCAGCGGAGTTGGAGGCCCTTCTCGTAAACCATCCCTCTATTGTAGAGGCAGCTATGGTCCCGTAAGTGCACAATTTAAATTTTGCAGTTTTATAGAGATCAATTAAATAAAATAAAATATTTATTCTTTTTAACTTTAATTCTTTAAAGAAAATACTTATTTAGTAACTGTTAATTTAACCGACATAGCTGATTTTTTTTTTTTCTAGAAAAAGTATAAACATGTGTTTGATAAATAATTTATTTTGTTTCTTCGGTTTGGTAACAATTTCTTTACTTGTATGTATGTTATAACATGGTAGTAAAAATTTCTGCTCTCTTTTATTTTGATTAAAAAAGATTATTTCTAGGAACTTTTTTTTATGAAAGCTTGTTTGATATTTAAAAAATTAATCGTAAAAATAGAAAATAGAAACTAGAAAGATGAAGGCCTAGATGATTTTATTTTTAATCTTTTACAATTAAGTTTTTTTTTTTTTTTGATTTTTTTTACAATTAAGCTTATTTACAAGCTTTTCGGTTTCCAGAAATAGCTTTTTGTTTTTTAGAAAATTTCCAGAAATAGGTTTTGAAAGCTTAATTTTGAATAAGAAATAAATTTTATGAAAATAACATTTTAAAACTTGAGAGATGAAAGGAACCATTGGATAAATTATTAGGTAATTCAGTCTCCTTATTTTGTGACATGTGACCATTCAACCCACATGGTTAACTAAGGTGGTTAACCTTTCTTTTTAATTTCTTTTTAATTCTTTCTATCAGTCGGTCTTTTTTTTTTTTTTTTATCGGTATTTGTCACACTCTTTTATTTATTTATTTATATATTTCTTTGATTTAAGATTTTCAATTGGTTGCTATATCTTTTTTTTTAATTTTGTTTTTCAATTTTACCACTTATATTTTCTTTTCTATTTTTTTTTTCTCTTTTCTATCAAGATTTCCTTGTCGATCAAGGCATTTTTTACCTTCAAATTTGGTTTCTTCTCTTTCTTCTTTTTTATATGGAGAAAAATAAGTAATTGTTAAACTAATTTGAGGGATACTTTTCAAATGCTTTTGTTAGTTAAACTTAAATTTTCACAAGCTTCCCCTCTAGCTTCAAATTCTAGCCTCTACTAAAATTAATGCAATTGCAATAGTTCATTACTGTTTTCGATCAAATCAATGATGTTTTTTTTTAGTTAAAATCTTAAATTCAAAAAAGAAAAGAAAAAACAAAGAGGTGACAAAATTTTGAGAAAAAGTAAAAAAGATATGTCTTAAAAGAAAGATAATGATGACTCTGGATAAGCAAAAGAAAAAAAGAAAAGAGATGAATATACTTAGTAGAATGATTAACCACGTGGGTTAAACTGCCACATGTCACAAAATAGAAGGGAGCCTCATCGTAGCCGAATCACCCAATAATTTTTGCAACAATTGACACTCACAGGCCAACATATCACACTTTTAATGATGACTTAAATTATCAAGACCTTTCTGTATTTGTGTTTAAATGATGAGAGAATAAACAATTTGAGCAAATTACAGGCAAAATGATGACGTTGCTGGTGAAGTTCCAGTGGCTTTCGTGGTTCGATCATCAGAAAATGAACTGACCGAGGAGGCAGTAAAAGAATTCATAGCAAAACAGGTTTAATTTGTGTATAAACAGAAATTAATTTTAAAAGCAAGTACATTAATACATTTGTTTTTATTTTTATTTTTTGTTTAATGCAAAACCATCAGGTTGTGTTCTACAAGAGATTGCAGAAAGTTTACTTTGTGCAAACAATTCCAAAATCACCCTCAGGGAAGATCTTGAGAAAGGAACTCAAAGCCAAGCTCTTAGTCTCACATGCTGCCTTCTAAAAGCCACAAAAGGGAGAGACAGCTGATTTATTAATTGAACTTTCATAAATGCTTGTCTGTGTGTCTTTACAAGCATCACCCATTTTGTTCCCACTTTAATTCAGTGATCTGCCATAAATTACAAGTTGTCAATTTATTTGTGTGGTTTAAAATTTGAGGGTAACCAGGTATCATATCATGGTTGGTACAATTTTGTGGGACAAAATAATACAAAAAATATATAAATATATAATTATTTGAGTACGAATCCACGAATCTCCATCTCTACCCTACATTAAACCTACCCCTTGAAAATCAA

mRNA sequence

ATGTGCTCGGCATTTGCAAAGGAGCCACCAATGCCAACAAAGCAGGGGCGGATTTATGAAGGGGTCGTGCCCCCCCTCAATTTAAACGTGGCACCAGCGGAGTTGGAGGCCCTTCTCGTAAACCATCCCTCTATTGTAGAGGCAGCTATGGTCCCGCAAAATGATGACGTTGCTGGTGAAGTTCCAGTGGCTTTCGTGGTTCGATCATCAGAAAATGAACTGACCGAGGAGGCAGTAAAAGAATTCATAGCAAAACAGGTTGTGTTCTACAAGAGATTGCAGAAAGTTTACTTTGTGCAAACAATTCCAAAATCACCCTCAGGGAAGATCTTGAGAAAGGAACTCAAAGCCAAGCTCTTAGTCTCACATGCTGCCTTCTAA

Coding sequence (CDS)

ATGTGCTCGGCATTTGCAAAGGAGCCACCAATGCCAACAAAGCAGGGGCGGATTTATGAAGGGGTCGTGCCCCCCCTCAATTTAAACGTGGCACCAGCGGAGTTGGAGGCCCTTCTCGTAAACCATCCCTCTATTGTAGAGGCAGCTATGGTCCCGCAAAATGATGACGTTGCTGGTGAAGTTCCAGTGGCTTTCGTGGTTCGATCATCAGAAAATGAACTGACCGAGGAGGCAGTAAAAGAATTCATAGCAAAACAGGTTGTGTTCTACAAGAGATTGCAGAAAGTTTACTTTGTGCAAACAATTCCAAAATCACCCTCAGGGAAGATCTTGAGAAAGGAACTCAAAGCCAAGCTCTTAGTCTCACATGCTGCCTTCTAA

Protein sequence

MCSAFAKEPPMPTKQGRIYEGVVPPLNLNVAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILRKELKAKLLVSHAAF

Homology

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BLAST of Spg029487 vs. NCBI nr
Match: XP_038901547.1 (4-coumarate--CoA ligase 3 isoform X2 [Benincasa hispida])

HSP 1 Score: 161.8 bits (408), Expect = 3.9e-36
Identity = 102/188 (54.26%), Postives = 109/188 (57.98%), Query Frame = 0


Query: 1   MCSAFAKEPPMPTKQG-------------------------------------------- 60
           MCSAFAKEPPMPTK G                                            
Sbjct: 363 MCSAFAKEPPMPTKSGSCGRVVRNSDLKVIDPQTGASLSYNQSGEICLRGPQVMKVDVEG 422

Query: 61  --------------------RIYEGVVPPLNLNVAPAELEALLVNHPSIVEAAMVPQNDD 120
                               R+ E ++      VAPAELE LLV HPSIV+AA+VPQNDD
Sbjct: 423 WLHTGDIGYIDDEDEIFIVDRVKE-IIKFKGFQVAPAELETLLVTHPSIVDAAVVPQNDD 482

Query: 121 VAGEVPVAFVVRSSENELTEEAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILRKELK 125
           VAGEVPVAFVV S+ NELTE+AVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILRKELK
Sbjct: 483 VAGEVPVAFVVPSTHNELTEDAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILRKELK 542

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BLAST of Spg029487 vs. NCBI nr
Match: XP_038901546.1 (4-coumarate--CoA ligase 3 isoform X1 [Benincasa hispida])

HSP 1 Score: 158.7 bits (400), Expect = 3.3e-35
Identity = 85/95 (89.47%), Postives = 89/95 (93.68%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           VAPAELE LLV HPSIV+AA+VPQNDDVAGEVPVAFVV S+ NELTE+AVKEFIAKQVVF
Sbjct: 467 VAPAELETLLVTHPSIVDAAVVPQNDDVAGEVPVAFVVPSTHNELTEDAVKEFIAKQVVF 526

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKELKAKLLVSHA 125
           YKRLQKVYFVQTIPKSPSGKILRKELKAKL VS A
Sbjct: 527 YKRLQKVYFVQTIPKSPSGKILRKELKAKLSVSPA 561

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BLAST of Spg029487 vs. NCBI nr
Match: XP_004151908.1 (4-coumarate--CoA ligase 2 [Cucumis sativus] >KAE8652343.1 hypothetical protein Csa_021810 [Cucumis sativus])

HSP 1 Score: 149.4 bits (376), Expect = 2.0e-32
Identity = 78/93 (83.87%), Postives = 87/93 (93.55%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           VAPAELEALLV H SIV+AA+VPQNDDVAGEVPVAF+V S+ NEL+EE+VKEFIAKQVVF
Sbjct: 465 VAPAELEALLVTHTSIVDAAVVPQNDDVAGEVPVAFIVPSTHNELSEESVKEFIAKQVVF 524

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKELKAKLLVS 123
           YKRL KVYFV+TIPKSPSGKILRKELKAKL ++
Sbjct: 525 YKRLHKVYFVKTIPKSPSGKILRKELKAKLSIT 557

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BLAST of Spg029487 vs. NCBI nr
Match: XP_022150871.1 (4-coumarate--CoA ligase 2-like, partial [Momordica charantia])

HSP 1 Score: 147.5 bits (371), Expect = 7.7e-32
Identity = 75/90 (83.33%), Postives = 87/90 (96.67%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           VAPAELEA+L+ HPSIV+AA+VPQ D+VAGEVPVAFVV+S+++ELTEEAVKEFIAKQVVF
Sbjct: 347 VAPAELEAILITHPSIVDAAVVPQKDEVAGEVPVAFVVKSTDHELTEEAVKEFIAKQVVF 406

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKELKAKL 120
           YKRLQ+V+FVQTIPK PSGKILRK+LKAKL
Sbjct: 407 YKRLQRVFFVQTIPKLPSGKILRKDLKAKL 436

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BLAST of Spg029487 vs. NCBI nr
Match: XP_008462779.1 (PREDICTED: 4-coumarate--CoA ligase 2 [Cucumis melo] >KAA0042418.1 4-coumarate--CoA ligase 2 [Cucumis melo var. makuwa] >TYK01002.1 4-coumarate--CoA ligase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 146.7 bits (369), Expect = 1.3e-31
Identity = 78/90 (86.67%), Postives = 84/90 (93.33%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           VAPAELEALLV H SIV+AA+VPQNDDVAGEVPVAFVV S++N LTEE+VKEFIAKQVVF
Sbjct: 468 VAPAELEALLVTHTSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVF 527

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKELKAKL 120
           YKRL KVYFV+TIPKSPSGK LRKELKAKL
Sbjct: 528 YKRLHKVYFVKTIPKSPSGKTLRKELKAKL 557

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BLAST of Spg029487 vs. ExPASy Swiss-Prot
Match: Q9S777 (4-coumarate--CoA ligase 3 OS=Arabidopsis thaliana OX=3702 GN=4CL3 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 6.1e-32
Identity = 70/90 (77.78%), Postives = 83/90 (92.22%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           V PAELE+LL+NH SI +AA+VPQND+VAGEVPVAFVVRS+ N++TEE VKE++AKQVVF
Sbjct: 471 VPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVF 530

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKELKAKL 120
           YKRL KV+FV +IPKSPSGKILRK+LKAKL
Sbjct: 531 YKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

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BLAST of Spg029487 vs. ExPASy Swiss-Prot
Match: P31687 (4-coumarate--CoA ligase 2 OS=Glycine max OX=3847 PE=2 SV=2)

HSP 1 Score: 135.6 bits (340), Expect = 4.0e-31
Identity = 72/90 (80.00%), Postives = 79/90 (87.78%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           V PAELE LLV+HPSI +AA+VPQ D  AGEVPVAFVVRS+  +LTEEAVKEFIAKQVVF
Sbjct: 465 VPPAELEGLLVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGFDLTEEAVKEFIAKQVVF 524

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKELKAKL 120
           YKRL KVYFV  IPKSPSGKILRK+L+AKL
Sbjct: 525 YKRLHKVYFVHAIPKSPSGKILRKDLRAKL 554

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BLAST of Spg029487 vs. ExPASy Swiss-Prot
Match: A0A2H5AIY4 (4-coumarate-CoA ligase 2 OS=Narcissus pseudonarcissus OX=39639 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 1.5e-30
Identity = 66/90 (73.33%), Postives = 80/90 (88.89%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           V PAELEALL+ HPSI +AA++PQND+VAGEVPVAFVV S  ++LTEE VKEFI+KQVVF
Sbjct: 475 VPPAELEALLLGHPSIADAAVIPQNDEVAGEVPVAFVVPSKSSDLTEEIVKEFISKQVVF 534

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKELKAKL 120
           YKR+ +VYF+  IPKSPSGKILRK+L+AK+
Sbjct: 535 YKRIHRVYFIHAIPKSPSGKILRKDLRAKV 564

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BLAST of Spg029487 vs. ExPASy Swiss-Prot
Match: Q42982 (4-coumarate--CoA ligase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CL2 PE=1 SV=2)

HSP 1 Score: 132.5 bits (332), Expect = 3.3e-30
Identity = 65/90 (72.22%), Postives = 82/90 (91.11%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           V PAELE+LL+ HPSI +AA+VPQ DDVAGEVPVAFVVR++++++TEE++KEFI+KQVVF
Sbjct: 477 VPPAELESLLIAHPSIADAAVVPQKDDVAGEVPVAFVVRAADSDITEESIKEFISKQVVF 536

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKELKAKL 120
           YKRL KV+F+  IPKS SGKILR+EL+AKL
Sbjct: 537 YKRLHKVHFIHAIPKSASGKILRRELRAKL 566

[Close]

BLAST of Spg029487 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.2e-29
Identity = 61/90 (67.78%), Postives = 84/90 (93.33%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           VAPAELEALL+NHP+I +AA+VP  D+ AGEVPVAFVVRS+ +++TE+ VK+F++KQV+F
Sbjct: 449 VAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGSDITEDEVKDFVSKQVIF 508

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKELKAKL 120
           YKR+++V+FV+T+PKSPSGKILRK+L+A+L
Sbjct: 509 YKRIKRVFFVETVPKSPSGKILRKDLRARL 538

[Close]

BLAST of Spg029487 vs. ExPASy TrEMBL
Match: A0A6J1DCS3 (4-coumarate--CoA ligase 2-like OS=Momordica charantia OX=3673 GN=LOC111018921 PE=4 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 3.7e-32
Identity = 75/90 (83.33%), Postives = 87/90 (96.67%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           VAPAELEA+L+ HPSIV+AA+VPQ D+VAGEVPVAFVV+S+++ELTEEAVKEFIAKQVVF
Sbjct: 347 VAPAELEAILITHPSIVDAAVVPQKDEVAGEVPVAFVVKSTDHELTEEAVKEFIAKQVVF 406

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKELKAKL 120
           YKRLQ+V+FVQTIPK PSGKILRK+LKAKL
Sbjct: 407 YKRLQRVFFVQTIPKLPSGKILRKDLKAKL 436

[Close]

BLAST of Spg029487 vs. ExPASy TrEMBL
Match: A0A5A7THZ8 (4-coumarate--CoA ligase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G002030 PE=4 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 6.3e-32
Identity = 78/90 (86.67%), Postives = 84/90 (93.33%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           VAPAELEALLV H SIV+AA+VPQNDDVAGEVPVAFVV S++N LTEE+VKEFIAKQVVF
Sbjct: 468 VAPAELEALLVTHTSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVF 527

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKELKAKL 120
           YKRL KVYFV+TIPKSPSGK LRKELKAKL
Sbjct: 528 YKRLHKVYFVKTIPKSPSGKTLRKELKAKL 557

[Close]

BLAST of Spg029487 vs. ExPASy TrEMBL
Match: A0A1S3CJB3 (4-coumarate--CoA ligase 2 OS=Cucumis melo OX=3656 GN=LOC103501067 PE=4 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 6.3e-32
Identity = 78/90 (86.67%), Postives = 84/90 (93.33%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           VAPAELEALLV H SIV+AA+VPQNDDVAGEVPVAFVV S++N LTEE+VKEFIAKQVVF
Sbjct: 468 VAPAELEALLVTHTSIVDAAVVPQNDDVAGEVPVAFVVPSTQNGLTEESVKEFIAKQVVF 527

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKELKAKL 120
           YKRL KVYFV+TIPKSPSGK LRKELKAKL
Sbjct: 528 YKRLHKVYFVKTIPKSPSGKTLRKELKAKL 557

[Close]

BLAST of Spg029487 vs. ExPASy TrEMBL
Match: A0A1U8B782 (4-coumarate--CoA ligase 2-like OS=Nelumbo nucifera OX=4432 GN=LOC104611494 PE=4 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 8.3e-32
Identity = 82/121 (67.77%), Postives = 93/121 (76.86%), Query Frame = 0


Query: 1   MCSAFAKEPPMPTKQGRIYEGV--VPPLNLNVAPAELEALLVNHPSIVEAAMVPQNDDVA 60
           MC  FAK+ P PTK G     V         V PAELEALLV+HPSI +AA+VPQ D VA
Sbjct: 381 MCPGFAKQ-PFPTKSGSCGTVVRNAEYKGFQVPPAELEALLVSHPSIADAAVVPQKDAVA 440

Query: 61  GEVPVAFVVRSSENELTEEAVKEFIAKQVVFYKRLQKVYFVQTIPKSPSGKILRKELKAK 120
           GEVPVAFVVRS+  +LTEE+VKEFIAKQVVFYK+L KVYFV  +PKSPSGKILRK+L+AK
Sbjct: 441 GEVPVAFVVRSNGFDLTEESVKEFIAKQVVFYKKLHKVYFVHAVPKSPSGKILRKDLRAK 500

[Close]

BLAST of Spg029487 vs. ExPASy TrEMBL
Match: A0A2N9ESS5 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS5735 PE=4 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 1.1e-31
Identity = 77/93 (82.80%), Postives = 84/93 (90.32%), Query Frame = 0


Query: 27  NLNVAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQ 86
           N  V PAELEALLV+HPSI +AA+VPQ D+VAGEVPVAFVVRS+ NELTEEAVKEFIAKQ
Sbjct: 489 NFFVPPAELEALLVSHPSIADAAVVPQKDNVAGEVPVAFVVRSNGNELTEEAVKEFIAKQ 548

Query: 87  VVFYKRLQKVYFVQTIPKSPSGKILRKELKAKL 120
           VVFYKRL KVYFV  IPKSPSGKILRK+L+AKL
Sbjct: 549 VVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKL 581

[Close]

BLAST of Spg029487 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 138.3 bits (347), Expect = 4.3e-33
Identity = 70/90 (77.78%), Postives = 83/90 (92.22%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           V PAELE+LL+NH SI +AA+VPQND+VAGEVPVAFVVRS+ N++TEE VKE++AKQVVF
Sbjct: 471 VPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVF 530

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKELKAKL 120
           YKRL KV+FV +IPKSPSGKILRK+LKAKL
Sbjct: 531 YKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

[Close]

BLAST of Spg029487 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 121.7 bits (304), Expect = 4.2e-28
Identity = 58/90 (64.44%), Postives = 78/90 (86.67%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           VAPAELEALL+ HP I + A+V   ++ AGEVPVAFVV+S ++EL+E+ VK+F++KQVVF
Sbjct: 468 VAPAELEALLIGHPDITDVAVVAMKEEAAGEVPVAFVVKSKDSELSEDDVKQFVSKQVVF 527

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKELKAKL 120
           YKR+ KV+F ++IPK+PSGKILRK+L+AKL
Sbjct: 528 YKRINKVFFTESIPKAPSGKILRKDLRAKL 557

[Close]

BLAST of Spg029487 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 114.8 bits (286), Expect = 5.1e-26
Identity = 54/90 (60.00%), Postives = 76/90 (84.44%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           VAPAELE+LL+ HP I + A+V   ++ AGEVPVAFVVRS ++ ++E+ +K+F++KQVVF
Sbjct: 461 VAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVF 520

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKELKAKL 120
           YKR+ KV+F  +IPK+PSGKILRK+L+A+L
Sbjct: 521 YKRINKVFFTDSIPKAPSGKILRKDLRARL 550

[Close]

BLAST of Spg029487 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 112.5 bits (280), Expect = 2.5e-25
Identity = 57/90 (63.33%), Postives = 75/90 (83.33%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           VAPAELEALL++HPSI +AA+V   D+VA EVPVAFV RS  ++LTE+ VK ++ KQVV 
Sbjct: 475 VAPAELEALLISHPSIDDAAVVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVH 534

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKELKAKL 120
           YKR++ V+F++ IPK+ SGKILRK+L+AKL
Sbjct: 535 YKRIKMVFFIEVIPKAVSGKILRKDLRAKL 564

[Close]

BLAST of Spg029487 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 104.0 bits (258), Expect = 9.1e-23
Identity = 51/86 (59.30%), Postives = 70/86 (81.40%), Query Frame = 0


Query: 30  VAPAELEALLVNHPSIVEAAMVPQNDDVAGEVPVAFVVRSSENELTEEAVKEFIAKQVVF 89
           VAPAELE LLV+HP I++A ++P  D+ AGEVP+AFVVRS  + +TE+ +++FIAKQV  
Sbjct: 451 VAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAP 510

Query: 90  YKRLQKVYFVQTIPKSPSGKILRKEL 116
           YKRL++V F+  +PKS +GKILR+EL
Sbjct: 511 YKRLRRVSFISLVPKSAAGKILRREL 536

The following BLAST results are available for this feature:

BLAST of Spg029487 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901547.1	3.9e-36	54.26	4-coumarate--CoA ligase 3 isoform X2 [Benincasa hispida]	[more]
XP_038901546.1	3.3e-35	89.47	4-coumarate--CoA ligase 3 isoform X1 [Benincasa hispida]	[more]
XP_004151908.1	2.0e-32	83.87	4-coumarate--CoA ligase 2 [Cucumis sativus] >KAE8652343.1 hypothetical protein C...	[more]
XP_022150871.1	7.7e-32	83.33	4-coumarate--CoA ligase 2-like, partial [Momordica charantia]	[more]
XP_008462779.1	1.3e-31	86.67	PREDICTED: 4-coumarate--CoA ligase 2 [Cucumis melo] >KAA0042418.1 4-coumarate--C...	[more]

BLAST of Spg029487 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S777	6.1e-32	77.78	4-coumarate--CoA ligase 3 OS=Arabidopsis thaliana OX=3702 GN=4CL3 PE=1 SV=1	[more]
P31687	4.0e-31	80.00	4-coumarate--CoA ligase 2 OS=Glycine max OX=3847 PE=2 SV=2	[more]
A0A2H5AIY4	1.5e-30	73.33	4-coumarate-CoA ligase 2 OS=Narcissus pseudonarcissus OX=39639 GN=4CL2 PE=2 SV=1	[more]
Q42982	3.3e-30	72.22	4-coumarate--CoA ligase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CL2 PE=1 ...	[more]
I3PB37	2.2e-29	67.78	4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1	[more]

BLAST of Spg029487 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DCS3	3.7e-32	83.33	4-coumarate--CoA ligase 2-like OS=Momordica charantia OX=3673 GN=LOC111018921 PE...	[more]
A0A5A7THZ8	6.3e-32	86.67	4-coumarate--CoA ligase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3CJB3	6.3e-32	86.67	4-coumarate--CoA ligase 2 OS=Cucumis melo OX=3656 GN=LOC103501067 PE=4 SV=1	[more]
A0A1U8B782	8.3e-32	67.77	4-coumarate--CoA ligase 2-like OS=Nelumbo nucifera OX=4432 GN=LOC104611494 PE=4 ...	[more]
A0A2N9ESS5	1.1e-31	82.80	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS5735 PE=4 SV=1	[more]

BLAST of Spg029487 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G65060.1	4.3e-33	77.78	4-coumarate:CoA ligase 3	[more]
AT1G51680.1	4.2e-28	64.44	4-coumarate:CoA ligase 1	[more]
AT3G21240.1	5.1e-26	60.00	4-coumarate:CoA ligase 2	[more]
AT3G21230.1	2.5e-25	63.33	4-coumarate:CoA ligase 5	[more]
AT4G05160.1	9.1e-23	59.30	AMP-dependent synthetase and ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.300.30		coord: 27..121 e-value: 3.1E-25 score: 90.7
None	No IPR available	PANTHER	PTHR24096:SF149	4-COUMARATE--COA LIGASE 2	coord: 29..119
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 29..119
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 29..119
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 34..109 e-value: 1.1E-16 score: 61.5

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg029487.1	Spg029487.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009698	phenylpropanoid metabolic process
biological_process	GO:0010584	pollen exine formation
molecular_function	GO:0016207	4-coumarate-CoA ligase activity
molecular_function	GO:0106290	trans-cinnamate-CoA ligase activity