Spg029481 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg029481
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	short-chain dehydrogenase TIC 32, chloroplastic-like
Location	scaffold12: 35665587 .. 35668705 (-)
RNA-Seq Expression	Spg029481
Synteny	Spg029481

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATGGAAGCTCCCAATTACCCATTTGAATAAACAAATCTCGTTTCTTTCTGTGTTTCTGAAATGACAAATATGTTATCCACCTTCTTTCTCTCTCCTCCCCCATTATAAATCCTGGTCCAAACCCCATTCCCTCTCCCCAACTCAACTCAACTCAACTCAATCCCTTCCCTTCTGCTTCTGAAATTGACGACACATGAACCGCCGCGTGCCCTGCCATTTCTGACACACAAACCACACCGCTCCCCAAATCCCAAATGCTCCCTTCCTTCAACTACCTCGCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCCACCGCCGACCTCGTCACCGCTTCCTCCCTCCCCCTCCCCCTCCCTTCCATCACCGCCATCATCACCGGCGCCACCTCCGGTATCGGAGCCGAGACCGCCCGTGTTCTCGCCAAACGCGGCGTCCGTCTCGTCCTCCCCTCCCGCAACCTCAAAGCCGCCGAGGAAGCCAAGGCCCGGATCCAGTCCGAATCCCCCGACTCCCGAATCATCATCATGGCTCTCGATTTGAGCTCGCTCTCCTCCGTTAGAACCTTCGTTGCCCAGTTTCTATCTCTTAATCTCCCTCTCAATCTCCTCATCAACAACGCCGGCAAATTCTCCCACCAGCATGCAATTTCCGAGGACGGGATCGAGATGACCTTCGCCACCAATTACTTGGGTAACTATTTTTTTTTTCAAATAATTTCCCTGAAGTTTTGGTCGTACGTAAAAATGGAATTTAATTTATTTTTATTTTGTTTTCAGGTCATTTTCTGTTGACGAAACTGTTGGTGAATAAGATGGCGGAGACGGCGAAAGCGACGGGGGTTGAAGGGCGGATTGTGAACGTCTCCTCGAGCATCCATGGCTGGTTTTCCGGCGACATGATCAAATATCTCGCCCAGATAAGCCGAAACAAAAGGTGATTTTTTTTAAAAAAAAATGATATGGTAAAATAAAATTTTGACTTGGGGGAGTATTATTATTATTTATTTATTTATTTAATTCTAATACAGTTAATGTGGGTGGATTCCCAGCAGGAATTACGATGCGACACGTGCTTACGCGCTCTCCAAGCTTGCCAACGTTCTGCACACCCACGAACTTGCTCGCAGATTGAAGGTTCTTTACATTTTATATTACTTTAATTACTGAATTTGGTCAATACTTATTATTATTATTATTATTTTTTTTTTTTATCTAAATTCAGACTAATTATTTTTTTTTTTTGTTTTGGTTTAGCAAATGGAAGCTAATGTTACTGTGAACTGCGTTCATCCGGGAATCGTCAGAACTCGACTCACAAGAGATCGTGAAGGTTTCATCACTGGTATGAATCTTCTTTAATTTTCTTCTTTTTTTTTTTCGGGTAATCTCAATATGAAAATTAAAAATAATTATTGAATTTGCAGATTTGGTGTTTTTTATGGCTTCCAAGCTGTTGAAGACAATCCCTCAGGTAAATAATCAAAACCCTCTCAATAATTTTGGTTAAATTACCTTTTTTTTTTTTAATAATGGAGTTCTTAACCTAGAAAAGGGTTAAATTTTAAATATTTTTTAAATAAATTTGTAATATTTTATTAATTATATATTTAATACTACTCTTTTTAAAGTTGCAGAACCAAAAAAAAAAAAAAGTTACAGAACCAATCAATCAAATGAGATGGGTGAATTTTTATTGAATGGATTTATTTTTTTAAAAATACCTTTTTGGTCTCTGAGTTTTGAGAAATAGGTGTGTTTGGTCCTCAAATTTTCAAAATAGACCTTTTTAGTACCTGAGTTTTGAGAAATAGGTTTAAAAGGTTTCTAAGATTTCAAAAGGGACCTTTTTAGTTCTAAGTTTTGACAAATTGGTTTAAAAGGTCCTTATATTATTCTTTTTCAAATTTTTAAATTAAAATTTTAAATTTTAAAACCATTCAATATTAAAAAATAATAAAAAATAATAATTTTAAAAAATTGAAAAAATAATATGAGAGATATTTTAAACATATTTCTCAAAACTCAATGACTAAAAATGTCTATTTTGAAAACTTAGGGATCTTTTAAACTTATTTCTCAAAACTCATAGACTAAAAAAATCTATTTTAAAAATTTAGGAACCAAACTCATCTATTCCTTAGAACTCAGGGACCAAAAAAGTATTTTTCCCTTTAAATTTTGTGAGTGAAATGTAAATTTTAAAAACATCATTTACATATATTCTAAATTTGCTACTAAAAACAATAAAAACCGTAGAATAATACAACTCCGTTTTTTGTAGACTTATTTTCAAATTTCTATTTTAAAATCACAATCCAACTCACAACTCACAATTTTGTTTTAAAAAAAGAAAGAAAAAAACAAAAACCAAACCTTTTTCTTTCCTTCGAAGTTCACGTTTTCCGTTTGTTTCCCGAGAAAATTAAAAAAAAAAAAAAGAAAAGAAAAGAAAAGAAAAGGCTATATTAGAACTGACACACAGGCCGTTCTGATCTTCTTCTTCCACAGGCGGCGGCAACGACATGCTACGTGGCGACGAGCCCGAGGCTAAGCCACGTCACCGGCAAGTATTTCTCAGACTGCAACGAAGCGTCGCCGTCGAAGCTCGCCGCCAGCCCCGCCGCAGCCGCCCGCCTCTGGTCCGCCTCCGAGATCATGGTCAACACAAACTCAAAACTCGTCTTCGATCCAAACAATGCCTTAGACTAATTTACTTTATTAAAAATCCAAATACCTCCAAAAAAAAAAAAAAGAAAAAAAAAAAAATAGATATGGCTTGTTTGGTTTAAAGAGAGAATCTTAGTCCAGAAATTAAGTATAAAAATATATTGGTAGATGGTAGGTAGACAAAAAGCAAGAAGCTAGCTAGCTAGTGGAGGGGAAGGAATATATTTACAACAAATGTAATATATAGTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTCAGAATATGGTAATGGATATAATCAAATAGCTAACTTTATTTTATGTGTATAATATATAGTTCCTTTTTTAAAATTTAATTTTGGTTGTCACATATGAGCCAAGCTAAGTGCTCTCTTTACACACTTTAATTATATCATTGGACCCATTCAAGTATTGGAGGATTTGGC

mRNA sequence

ATGCTCCCTTCCTTCAACTACCTCGCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCCACCGCCGACCTCGTCACCGCTTCCTCCCTCCCCCTCCCCCTCCCTTCCATCACCGCCATCATCACCGGCGCCACCTCCGGTATCGGAGCCGAGACCGCCCGTGTTCTCGCCAAACGCGGCGTCCGTCTCGTCCTCCCCTCCCGCAACCTCAAAGCCGCCGAGGAAGCCAAGGCCCGGATCCAGTCCGAATCCCCCGACTCCCGAATCATCATCATGGCTCTCGATTTGAGCTCGCTCTCCTCCGTTAGAACCTTCGTTGCCCAGTTTCTATCTCTTAATCTCCCTCTCAATCTCCTCATCAACAACGCCGGCAAATTCTCCCACCAGCATGCAATTTCCGAGGACGGGATCGAGATGACCTTCGCCACCAATTACTTGGGTCATTTTCTGTTGACGAAACTGTTGGTGAATAAGATGGCGGAGACGGCGAAAGCGACGGGGGTTGAAGGGCGGATTGTGAACGTCTCCTCGAGCATCCATGGCTGGTTTTCCGGCGACATGATCAAATATCTCGCCCAGATAAGCCGAAACAAAAGGAATTACGATGCGACACGTGCTTACGCGCTCTCCAAGCTTGCCAACGTTCTGCACACCCACGAACTTGCTCGCAGATTGAAGCAAATGGAAGCTAATGTTACTGTGAACTGCGTTCATCCGGGAATCGTCAGAACTCGACTCACAAGAGATCGTGAAGGTTTCATCACTGATTTGGTGTTTTTTATGGCTTCCAAGCTGTTGAAGACAATCCCTCAGGCGGCGGCAACGACATGCTACGTGGCGACGAGCCCGAGGCTAAGCCACGTCACCGGCAAGTATTTCTCAGACTGCAACGAAGCGTCGCCGTCGAAGCTCGCCGCCAGCCCCGCCGCAGCCGCCCGCCTCTGGTCCGCCTCCGAGATCATGGTCAACACAAACTCAAAACTCGTCTTCGATCCAAACAATGCCTTAGACTAA

Coding sequence (CDS)

ATGCTCCCTTCCTTCAACTACCTCGCCGGCTCCCCCGGCCCCTCCGGCTTCGGCTCCAAATCCACCGCCGACCTCGTCACCGCTTCCTCCCTCCCCCTCCCCCTCCCTTCCATCACCGCCATCATCACCGGCGCCACCTCCGGTATCGGAGCCGAGACCGCCCGTGTTCTCGCCAAACGCGGCGTCCGTCTCGTCCTCCCCTCCCGCAACCTCAAAGCCGCCGAGGAAGCCAAGGCCCGGATCCAGTCCGAATCCCCCGACTCCCGAATCATCATCATGGCTCTCGATTTGAGCTCGCTCTCCTCCGTTAGAACCTTCGTTGCCCAGTTTCTATCTCTTAATCTCCCTCTCAATCTCCTCATCAACAACGCCGGCAAATTCTCCCACCAGCATGCAATTTCCGAGGACGGGATCGAGATGACCTTCGCCACCAATTACTTGGGTCATTTTCTGTTGACGAAACTGTTGGTGAATAAGATGGCGGAGACGGCGAAAGCGACGGGGGTTGAAGGGCGGATTGTGAACGTCTCCTCGAGCATCCATGGCTGGTTTTCCGGCGACATGATCAAATATCTCGCCCAGATAAGCCGAAACAAAAGGAATTACGATGCGACACGTGCTTACGCGCTCTCCAAGCTTGCCAACGTTCTGCACACCCACGAACTTGCTCGCAGATTGAAGCAAATGGAAGCTAATGTTACTGTGAACTGCGTTCATCCGGGAATCGTCAGAACTCGACTCACAAGAGATCGTGAAGGTTTCATCACTGATTTGGTGTTTTTTATGGCTTCCAAGCTGTTGAAGACAATCCCTCAGGCGGCGGCAACGACATGCTACGTGGCGACGAGCCCGAGGCTAAGCCACGTCACCGGCAAGTATTTCTCAGACTGCAACGAAGCGTCGCCGTCGAAGCTCGCCGCCAGCCCCGCCGCAGCCGCCCGCCTCTGGTCCGCCTCCGAGATCATGGTCAACACAAACTCAAAACTCGTCTTCGATCCAAACAATGCCTTAGACTAA

Protein sequence

MLPSFNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKRGVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLLINNAGKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIHGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHPGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEASPSKLAASPAAAARLWSASEIMVNTNSKLVFDPNNALD

Homology

[Close]

BLAST of Spg029481 vs. NCBI nr
Match: KAG7035489.1 (Short-chain dehydrogenase TIC 32, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 587.4 bits (1513), Expect = 7.8e-164
Identity = 311/338 (92.01%), Postives = 319/338 (94.38%), Query Frame = 0


Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VTASS PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLL 120
           GVRL+LPSRNLKAAEE KARI SE  DS+II+M LDLSSLSSV  FV QF SLNLPLNLL
Sbjct: 61  GVRLILPSRNLKAAEETKARILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH HAISEDGIEMTFATNYLGHFLLTKLLVN MA+TAKATGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHDHAISEDGIEMTFATNYLGHFLLTKLLVNTMADTAKATGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHP 240
           HGWFSGDMIKYL QISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANV+VNCVHP
Sbjct: 181 HGWFSGDMIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVSVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDPNNALD 339
           SPSKLAASP AAARLWSASEIMVNT SKLVFDPNN L+
Sbjct: 301 SPSKLAASPTAAARLWSASEIMVNTKSKLVFDPNNGLE 337

[Close]

BLAST of Spg029481 vs. NCBI nr
Match: XP_023534460.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 586.3 bits (1510), Expect = 1.7e-163
Identity = 311/338 (92.01%), Postives = 318/338 (94.08%), Query Frame = 0


Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VTASS PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLL 120
           GVRL+LPSRNLKAAEE KARI SE  DS+II+M LDLSSLSSV  FV QF SLNLPLNLL
Sbjct: 61  GVRLILPSRNLKAAEETKARILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH HAISEDGIEMTFATNYLGHFLLTKLLVN MAETAKATGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHDHAISEDGIEMTFATNYLGHFLLTKLLVNTMAETAKATGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHP 240
           HGWFSGDMIKYL QISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANV+VNCVHP
Sbjct: 181 HGWFSGDMIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVSVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRL HVTGKYFSDCNEA
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLIHVTGKYFSDCNEA 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDPNNALD 339
           SPSKLAASP AAARLWSASEIMVNT SKLVFDPNN L+
Sbjct: 301 SPSKLAASPTAAARLWSASEIMVNTKSKLVFDPNNGLE 337

[Close]

BLAST of Spg029481 vs. NCBI nr
Match: XP_022957741.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 585.5 bits (1508), Expect = 3.0e-163
Identity = 311/338 (92.01%), Postives = 318/338 (94.08%), Query Frame = 0


Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VTASS PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLL 120
            VRL+LPSRNLKAAEE KARI SE  DS+II+M LDLSSLSSV  FV QF SLNLPLNLL
Sbjct: 61  RVRLILPSRNLKAAEETKARILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH HAISEDGIEMTFATNYLGHFLLTKLLVN MAETAKATGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHDHAISEDGIEMTFATNYLGHFLLTKLLVNTMAETAKATGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHP 240
           HGWFSGDMIKYL QISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANV+VNCVHP
Sbjct: 181 HGWFSGDMIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVSVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDPNNALD 339
           SPSKLAASP AAARLWSASEIMVNT SKLVFDPNN L+
Sbjct: 301 SPSKLAASPTAAARLWSASEIMVNTKSKLVFDPNNGLE 337

[Close]

BLAST of Spg029481 vs. NCBI nr
Match: XP_022995788.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 583.2 bits (1502), Expect = 1.5e-162
Identity = 308/338 (91.12%), Postives = 317/338 (93.79%), Query Frame = 0


Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VTASS PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLL 120
           GVRL+LPSRNLKAAEE K+RI SE  DS+II+M LDLSSLSSV  FV QF SLNLPLNLL
Sbjct: 61  GVRLILPSRNLKAAEETKSRILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH+HAISEDGIEMTFATNYLGHFLLTKLLVN M ETAK TGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETAKETGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHP 240
           HGWFSGDMIKYL QISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHP
Sbjct: 181 HGWFSGDMIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDPNNALD 339
           SPSKLAASP AAARLWS SEIMVN+ SKLVFDPNN L+
Sbjct: 301 SPSKLAASPTAAARLWSTSEIMVNSKSKLVFDPNNGLE 337

[Close]

BLAST of Spg029481 vs. NCBI nr
Match: KAG6605574.1 (Short-chain dehydrogenase TIC 32 A, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 582.8 bits (1501), Expect = 1.9e-162
Identity = 311/339 (91.74%), Postives = 319/339 (94.10%), Query Frame = 0


Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VTASS PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLL 120
           GVRL+LPSRNLKAAEE KARI SE  DS+II+M LDLSSLSSV  FV QF SLNLPLNLL
Sbjct: 61  GVRLILPSRNLKAAEETKARILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH HAISEDGIEMTFATNYLGHFLLTKLLVN MA+TAKATGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHDHAISEDGIEMTFATNYLGHFLLTKLLVNTMADTAKATGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNK-RNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVH 240
           HGWFSGDMIKYL QISRNK RNYDATRAYALSKLANVLHTHELARRLKQMEANV+VNCVH
Sbjct: 181 HGWFSGDMIKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRLKQMEANVSVNCVH 240

Query: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNE 300
           PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNE
Sbjct: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNE 300

Query: 301 ASPSKLAASPAAAARLWSASEIMVNTNSKLVFDPNNALD 339
           ASPSKLAASP AAARLWSASEIMVNT SKLVFDPNN L+
Sbjct: 301 ASPSKLAASPTAAARLWSASEIMVNTKSKLVFDPNNGLE 338

[Close]

BLAST of Spg029481 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.0e-70
Identity = 156/323 (48.30%), Postives = 208/323 (64.40%), Query Frame = 0


Query: 5   FNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKRGVRL 64
           +  + G  G SG+GS STA+ VT S   +    +TAIITG TSGIG E ARVL  RG  +
Sbjct: 4   YGMVTGKAGKSGYGSASTAEDVTHS---IDAKHLTAIITGGTSGIGLEAARVLGMRGAHV 63

Query: 65  VLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLLINNA 124
           ++ SRN KAA ++K  I    P++RI  + LDLSS+ SVR+F+ QFL+LN+PLN+LINNA
Sbjct: 64  IIASRNTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNA 123

Query: 125 GKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIH--G 184
           G       +SEDGIE  FATN++GHFLLT LL++KM  +A+ +G+EGRIVN+SS  H   
Sbjct: 124 GVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYT 183

Query: 185 WFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHPGI 244
           +  G M  Y+    R    Y   +AY  SKLAN+LH++ L+R+L++   N+T+N VHPG+
Sbjct: 184 YTEGIMFDYINDPDR----YSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGL 243

Query: 245 VRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEASP 304
           + T L R   G    ++  M+  L K IPQ AATTCYVA  P L  VTGKYF+DCN  +P
Sbjct: 244 ITTNLFR-HSGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFADCNVTTP 303

Query: 305 SKLAASPAAAARLWSASEIMVNT 326
           S  A     A +LW  S  +V +
Sbjct: 304 SNFAIDTTLADKLWDFSIKLVES 318

[Close]

BLAST of Spg029481 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.3e-70
Identity = 155/323 (47.99%), Postives = 209/323 (64.71%), Query Frame = 0


Query: 5   FNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKRGVRL 64
           +  + G  G SG+GS STA+ VT S   +    +TAIITG TSGIG E ARVL  RG  +
Sbjct: 4   YGMVTGKAGKSGYGSASTAEDVTHS---IDAKHLTAIITGGTSGIGLEAARVLGMRGAHV 63

Query: 65  VLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLLINNA 124
           ++ +RN KAA ++K  I    P++RI  + LDLSS+ SVR+F+ QFL+LN+PLN+LINNA
Sbjct: 64  IIAARNTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNA 123

Query: 125 GKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIH--G 184
           G       +SEDGIE  FATN++GHFLLT LL++KM  +A+ +G+EGRIVN+SS  H   
Sbjct: 124 GVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYT 183

Query: 185 WFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHPGI 244
           +  G M  Y+    R    Y   +AY  SKLAN+LH++ L+R+L++   N+T+N VHPG+
Sbjct: 184 YTEGIMFDYINDPDR----YSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGL 243

Query: 245 VRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEASP 304
           + T L R   G    ++  M+  L K IPQ AATTCYVA  P L  VTGKYF+DCN  +P
Sbjct: 244 ITTNLFR-HSGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTDCNVTTP 303

Query: 305 SKLAASPAAAARLWSASEIMVNT 326
           S  A     A +LW  S  +V++
Sbjct: 304 SNFAIDTTLADKLWDFSIKLVDS 318

[Close]

BLAST of Spg029481 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 3.3e-64
Identity = 141/318 (44.34%), Postives = 204/318 (64.15%), Query Frame = 0


Query: 10  GSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKRGVRLVLPSR 69
           GS G SGF S+STA+ VT     +    +TAI+TGA+SGIG ETARVL+ RGV +V+  R
Sbjct: 5   GSKGASGFSSRSTAEEVTHG---VDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAVR 64

Query: 70  NLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLLINNAGKFSH 129
           N  +  + K  I  + P +++ +M LDLSS+ SVR F +++ S  LPLNLLINNAG  + 
Sbjct: 65  NTDSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMAC 124

Query: 130 QHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIHGWFSGDMI 189
              +S+D IE+ FATN+LGHFLLTKLL++ M  T++ +  EGRIVN+SS  H +   + +
Sbjct: 125 PFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEGV 184

Query: 190 KYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHPGIVRTRLTR 249
           ++      +K +Y + RAY  SKL NVLH +EL ++LK+   N+T N +HPG + T L R
Sbjct: 185 RF--DKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGR 244

Query: 250 DREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEASPSKLAASP 309
               ++   V  +A  +LK++PQ AATTCYVA +P+++ V+G+YF D N A P  L    
Sbjct: 245 YFNPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDT 304

Query: 310 AAAARLWSASEIMVNTNS 328
             A ++W  S  + ++ S
Sbjct: 305 ELAKKVWDFSTKLTDSQS 317

[Close]

BLAST of Spg029481 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 3.9e-57
Identity = 131/307 (42.67%), Postives = 189/307 (61.56%), Query Frame = 0


Query: 13  GPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKRGVRLVLPSRNLK 72
           G SGF   STA+ VT     +    +TAI+TGA+SGIGAET RVLA RG  +++  RN+ 
Sbjct: 9   GVSGFSGSSTAEQVTHG---IDATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMV 68

Query: 73  AAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLLINNAGKFSHQHA 132
           AA++ K  I  + P +++  + LDLSSL SV+ F ++F S   PLN+LINNAG  +    
Sbjct: 69  AAKDVKDTILKDIPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPFK 128

Query: 133 ISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIHGWFSGDMIKYL 192
           +S+D IE+ FATN++GHFLLT LL++ M +T + +  EGRIVNV+S  H +   + I++ 
Sbjct: 129 LSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRF- 188

Query: 193 AQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHPGIVRTRLTRDRE 252
                ++ +Y+  RAY  SKLANVLH ++L + LK+   N+T N +HPG + T L R   
Sbjct: 189 -DKINDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFRHNS 248

Query: 253 GFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEASPSKLAASPAAA 312
             +  L+  +   +LK + Q AATTCYVA  P++  V+G+YFSD N    +        A
Sbjct: 249 A-VNGLINVIGKLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKDVDLA 308

Query: 313 ARLWSAS 320
            +LW  S
Sbjct: 309 KKLWDFS 309

[Close]

BLAST of Spg029481 vs. ExPASy Swiss-Prot
Match: P59837 (Retinol dehydrogenase 12 OS=Bos taurus OX=9913 GN=RDH12 PE=2 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 9.3e-43
Identity = 114/279 (40.86%), Postives = 164/279 (58.78%), Query Frame = 0


Query: 41  IITGATSGIGAETARVLAKRGVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSL 100
           +ITGA +GIG ETAR LA+RG R+ +  R++   E A + IQ+++ +S++++  LDLS  
Sbjct: 43  VITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIQADTKNSQVLVRKLDLSDT 102

Query: 101 SSVRTFVAQFLSLNLPLNLLINNAGKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKM 160
            S+R F   FL+    L++LINNAG     ++ + DG E   A N+LGHFLLT LL+ ++
Sbjct: 103 KSIRAFAEGFLAEEKQLHILINNAGVMLCPYSKTADGFETHLAVNHLGHFLLTHLLLGRL 162

Query: 161 AETAKATGVEGRIVNVSSSIHGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTH 220
            E+A A     R+VN+SS  H      + K      +  + Y+   AY  SKLANVL T 
Sbjct: 163 KESAPA-----RVVNLSSVAH-----HLGKIRFHDLQGDKYYNLGFAYCHSKLANVLFTR 222

Query: 221 ELARRLKQMEANVTVNCVHPGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYV 280
           ELA+RLK     VT   VHPGIVR++L   R  F+  L++ + S  LKT  + A T+ + 
Sbjct: 223 ELAKRLK--GTGVTTYAVHPGIVRSKLV--RHSFLLCLLWRLFSPFLKTTWEGAQTSLHC 282

Query: 281 ATSPRLSHVTGKYFSDCNEASPSKLAASPAAAARLWSAS 320
           A +  L  ++GKYFSDC +   S  A +   A RLW+ S
Sbjct: 283 ALAEGLEPLSGKYFSDCKKTWVSPRARNNKTAERLWNVS 307

[Close]

BLAST of Spg029481 vs. ExPASy TrEMBL
Match: A0A6J1H032 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459194 PE=3 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.4e-163
Identity = 311/338 (92.01%), Postives = 318/338 (94.08%), Query Frame = 0


Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VTASS PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLL 120
            VRL+LPSRNLKAAEE KARI SE  DS+II+M LDLSSLSSV  FV QF SLNLPLNLL
Sbjct: 61  RVRLILPSRNLKAAEETKARILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH HAISEDGIEMTFATNYLGHFLLTKLLVN MAETAKATGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHDHAISEDGIEMTFATNYLGHFLLTKLLVNTMAETAKATGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHP 240
           HGWFSGDMIKYL QISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANV+VNCVHP
Sbjct: 181 HGWFSGDMIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVSVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDPNNALD 339
           SPSKLAASP AAARLWSASEIMVNT SKLVFDPNN L+
Sbjct: 301 SPSKLAASPTAAARLWSASEIMVNTKSKLVFDPNNGLE 337

[Close]

BLAST of Spg029481 vs. ExPASy TrEMBL
Match: A0A6J1K910 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491218 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 7.1e-163
Identity = 308/338 (91.12%), Postives = 317/338 (93.79%), Query Frame = 0


Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VTASS PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLL 120
           GVRL+LPSRNLKAAEE K+RI SE  DS+II+M LDLSSLSSV  FV QF SLNLPLNLL
Sbjct: 61  GVRLILPSRNLKAAEETKSRILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH+HAISEDGIEMTFATNYLGHFLLTKLLVN M ETAK TGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETAKETGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHP 240
           HGWFSGDMIKYL QISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHP
Sbjct: 181 HGWFSGDMIKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDPNNALD 339
           SPSKLAASP AAARLWS SEIMVN+ SKLVFDPNN L+
Sbjct: 301 SPSKLAASPTAAARLWSTSEIMVNSKSKLVFDPNNGLE 337

[Close]

BLAST of Spg029481 vs. ExPASy TrEMBL
Match: A0A6J1H1D2 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459194 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.5e-162
Identity = 311/339 (91.74%), Postives = 318/339 (93.81%), Query Frame = 0


Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VTASS PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLL 120
            VRL+LPSRNLKAAEE KARI SE  DS+II+M LDLSSLSSV  FV QF SLNLPLNLL
Sbjct: 61  RVRLILPSRNLKAAEETKARILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH HAISEDGIEMTFATNYLGHFLLTKLLVN MAETAKATGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHDHAISEDGIEMTFATNYLGHFLLTKLLVNTMAETAKATGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNK-RNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVH 240
           HGWFSGDMIKYL QISRNK RNYDATRAYALSKLANVLHTHELARRLKQMEANV+VNCVH
Sbjct: 181 HGWFSGDMIKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRLKQMEANVSVNCVH 240

Query: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNE 300
           PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNE
Sbjct: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNE 300

Query: 301 ASPSKLAASPAAAARLWSASEIMVNTNSKLVFDPNNALD 339
           ASPSKLAASP AAARLWSASEIMVNT SKLVFDPNN L+
Sbjct: 301 ASPSKLAASPTAAARLWSASEIMVNTKSKLVFDPNNGLE 338

[Close]

BLAST of Spg029481 vs. ExPASy TrEMBL
Match: A0A6J1JZY1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491218 PE=3 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 1.7e-161
Identity = 308/339 (90.86%), Postives = 317/339 (93.51%), Query Frame = 0


Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SF YLAGSPGPSGFGSKSTA+ VTASS PLPLPS+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MLDSFYYLAGSPGPSGFGSKSTAEQVTASS-PLPLPSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLL 120
           GVRL+LPSRNLKAAEE K+RI SE  DS+II+M LDLSSLSSV  FV QF SLNLPLNLL
Sbjct: 61  GVRLILPSRNLKAAEETKSRILSECSDSQIIVMPLDLSSLSSVTNFVHQFQSLNLPLNLL 120

Query: 121 INNAGKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH+HAISEDGIEMTFATNYLGHFLLTKLLVN M ETAK TGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAISEDGIEMTFATNYLGHFLLTKLLVNTMVETAKETGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNK-RNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVH 240
           HGWFSGDMIKYL QISRNK RNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVH
Sbjct: 181 HGWFSGDMIKYLGQISRNKSRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVH 240

Query: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNE 300
           PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNE
Sbjct: 241 PGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNE 300

Query: 301 ASPSKLAASPAAAARLWSASEIMVNTNSKLVFDPNNALD 339
           ASPSKLAASP AAARLWS SEIMVN+ SKLVFDPNN L+
Sbjct: 301 ASPSKLAASPTAAARLWSTSEIMVNSKSKLVFDPNNGLE 338

[Close]

BLAST of Spg029481 vs. ExPASy TrEMBL
Match: A0A5D3C462 (Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001850 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.0e-157
Identity = 297/338 (87.87%), Postives = 315/338 (93.20%), Query Frame = 0


Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKR 60
           ML SFNYL+GSPGPSGFGSKSTA+ VT SSLP   PS TAIITGATSGIGAETAR+LAKR
Sbjct: 1   MLLSFNYLSGSPGPSGFGSKSTAEHVT-SSLP---PSFTAIITGATSGIGAETARILAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLL 120
           G RL+LPSRNLKAAEE KARI+SE  DS II+M+LDLSSLSSVRTFV+QF SLNLPLNLL
Sbjct: 61  GARLILPSRNLKAAEETKARIESECSDSEIIVMSLDLSSLSSVRTFVSQFESLNLPLNLL 120

Query: 121 INNAGKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGKFSH+HAI+EDGIEMTFATNYLGHFLLTKLLVNKM ETAK TGVEGRIVNVSSSI
Sbjct: 121 INNAGKFSHEHAITEDGIEMTFATNYLGHFLLTKLLVNKMVETAKVTGVEGRIVNVSSSI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHP 240
           H WFSGD++KYL QISRNKRNYDATRAYALSKLANVLHTHELARR KQM ANVTVNCVHP
Sbjct: 181 HSWFSGDILKYLGQISRNKRNYDATRAYALSKLANVLHTHELARRFKQMGANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVAT+PRL HVTGKYF DCNE+
Sbjct: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATNPRLRHVTGKYFVDCNES 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDPNNALD 339
           SPSKLAASP+ AARLWSASEIMVN +SKL+FDPNNAL+
Sbjct: 301 SPSKLAASPSEAARLWSASEIMVNASSKLLFDPNNALE 334

[Close]

BLAST of Spg029481 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 457.2 bits (1175), Expect = 1.1e-128
Identity = 236/333 (70.87%), Postives = 283/333 (84.98%), Query Frame = 0


Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKR 60
           M+ +  YL G+ G SGFGSKSTA+ VT +     L SITA+ITGATSGIGAETARVLAKR
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTEN---CDLRSITAVITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLL 120
           G RL+ P+RN+KAAEEAK RI SE P++ I++M LDLSS++SVR FVA F SL+LPLNLL
Sbjct: 61  GARLIFPARNVKAAEEAKERIVSEFPETEIVVMKLDLSSIASVRNFVADFESLDLPLNLL 120

Query: 121 INNAGKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGK +H+HAISEDGIEMTFATNYLGHFLLT LL+NKM +TA+ TGV+GRIVNV+S I
Sbjct: 121 INNAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSGI 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHP 240
           HGWFSGD+I+YL  IS+ K  +DATRAYALSKLANVLHT EL+ RL+++ ANVTVNCVHP
Sbjct: 181 HGWFSGDLIEYLRLISQPKCQFDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           G+VRTRLTRDREG +TDLVFF+ASKL+KT+PQAAATTCYVAT+PRL +V+GKYF+DCNE 
Sbjct: 241 GVVRTRLTRDREGLLTDLVFFLASKLVKTVPQAAATTCYVATNPRLVNVSGKYFTDCNET 300

Query: 301 SPSKLAASPAAAARLWSASEIMVNTNSKLVFDP 334
           +PS L  + + A +LW+ASEI+V  +SK  FDP
Sbjct: 301 TPSGLGTNSSEATKLWAASEILVTQHSKTSFDP 330

[Close]

BLAST of Spg029481 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 454.9 bits (1169), Expect = 5.6e-128
Identity = 237/324 (73.15%), Postives = 276/324 (85.19%), Query Frame = 0


Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKR 60
           M+ +  +L GS GPSGFGS+STAD VT +S    L S+TAIITGATSGIGAETARVLAKR
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVTCNS---DLRSLTAIITGATSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLL 120
           G RLVLP+R++K AEE KARI SE PD+ II+M LDLSSL+SVR FV  F SLNLPLN+L
Sbjct: 61  GARLVLPARSVKTAEETKARILSEFPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNIL 120

Query: 121 INNAGKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAGK++H+HA+SEDG+EMTFATNYLGHFLLTKLL+ KM ETA  TGV+GRIVNV+S +
Sbjct: 121 INNAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSVV 180

Query: 181 HGWFSGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHP 240
           H WFSGDM++YLA ISRN RNYDATRAYALSKLANVLHT EL+R L +M+ANVT NCVHP
Sbjct: 181 HSWFSGDMLQYLADISRNNRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVHP 240

Query: 241 GIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEA 300
           GIV+TRLTRDREG +TDLVFF+ SKLLK++PQAAATTCYVATSPRL +V GKYFSDCNEA
Sbjct: 241 GIVKTRLTRDREGVVTDLVFFLTSKLLKSVPQAAATTCYVATSPRLRNVCGKYFSDCNEA 300

Query: 301 SPSKLAASPAAAARLWSASEIMVN 325
             SK  +    A RLW+AS+++V+
Sbjct: 301 RSSKSGSCNLKAQRLWTASDLLVS 321

[Close]

BLAST of Spg029481 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 319.3 bits (817), Expect = 3.7e-87
Identity = 179/327 (54.74%), Postives = 230/327 (70.34%), Query Frame = 0


Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKR 60
           M  +  YLAG  GP+GFGS+STA+ VT  S   P   +TAIITG TSGIGAETARVLAKR
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSF-FPCSHLTAIITGGTSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLL 120
           GVR+V+  R++K AE  K RI  E+P++ II+  +DLSSLSSV  F +QFLS +LPLN+L
Sbjct: 61  GVRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNIL 120

Query: 121 INNAGKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAG FS     SE+ IE+TFATN+LGH+LLT++L+ KM +TA+ +G+EGRI+N+SS I
Sbjct: 121 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 180

Query: 181 HGWFSGDMI---KYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNC 240
           H W   D     K L  ISR    Y+ TRAYA SKLA +LH   L+++LK   ANVT+N 
Sbjct: 181 HNWVKPDCFSFPKLLHPISR----YNGTRAYAQSKLATILHAKALSKQLKDRNANVTINA 240

Query: 241 VHPGIVRTRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDC 300
           VHPGIV+T + R  +G  TD +F +ASKLLK+I Q AATTCYVA S     ++GKYF+DC
Sbjct: 241 VHPGIVKTGIIRAHKGLFTDSLFLIASKLLKSISQGAATTCYVALSNETKGLSGKYFADC 300

Query: 301 NEASPSKLAASPAAAARLWSASEIMVN 325
           NE + S LA     A +L + S  +++
Sbjct: 301 NETNCSDLANDEYVALKLCTQSRALIH 322

[Close]

BLAST of Spg029481 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 283.5 bits (724), Expect = 2.2e-76
Identity = 160/324 (49.38%), Postives = 219/324 (67.59%), Query Frame = 0


Query: 5   FNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKRGVRL 64
           ++ + G  GPSGFGS STA+ VT     +   ++TAIITG T GIG ETARVL+KRG  +
Sbjct: 4   YSLITGRRGPSGFGSASTAEEVTQG---IDATNLTAIITGGTGGIGMETARVLSKRGAHV 63

Query: 65  VLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLLINNA 124
           V+ +RN+ AAE AK  I  ++ ++R+ ++ LDLSS+ S++ FV +F +L+LPLNLLINNA
Sbjct: 64  VIGARNMGAAENAKTEILRQNANARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINNA 123

Query: 125 GKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSIHGWF 184
           G     + +SEDGIE+ FATN++GHFLLT LL++ M  TAK +GVEGRI+NVSS  H + 
Sbjct: 124 GVMFCPYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIYT 183

Query: 185 SGDMIKYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNCVHPGIVR 244
             + I++      +  +Y   RAY  SKLAN+LH +EL+R+L++   N+T N VHPG++ 
Sbjct: 184 YQEGIQF--DSINDICSYSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHPGLIL 243

Query: 245 TRLTRDREGFITDLVFFMASKLLKTIPQAAATTCYVATSPRLSHVTGKYFSDCNEASPSK 304
           T L +     +  L FF +  L K IPQ AATTCYVA  P +  VTGKYF+DCNE +PSK
Sbjct: 244 TNLFQHTALLMRFLKFF-SFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEVTPSK 303

Query: 305 LAASPAAAARLWSASEIMVNTNSK 329
           LA     A +LW  S  ++N+ SK
Sbjct: 304 LARDETLAQKLWDFSVKLINSVSK 321

[Close]

BLAST of Spg029481 vs. TAIR 10
Match: AT5G50130.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 279.3 bits (713), Expect = 4.2e-75
Identity = 156/275 (56.73%), Postives = 198/275 (72.00%), Query Frame = 0


Query: 1   MLPSFNYLAGSPGPSGFGSKSTADLVTASSLPLPLPSITAIITGATSGIGAETARVLAKR 60
           M  +  YLAG  GP+GFGS+STA+ VT  S   P   +TAIITG TSGIGAETARVLAKR
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSF-FPCSHLTAIITGGTSGIGAETARVLAKR 60

Query: 61  GVRLVLPSRNLKAAEEAKARIQSESPDSRIIIMALDLSSLSSVRTFVAQFLSLNLPLNLL 120
           GVR+V+  R++K AE  K RI  E+P++ II+  +DLSSLSSV  F +QFLS +LPLN+L
Sbjct: 61  GVRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNIL 120

Query: 121 INNAGKFSHQHAISEDGIEMTFATNYLGHFLLTKLLVNKMAETAKATGVEGRIVNVSSSI 180
           INNAG FS     SE+ IE+TFATN+LGH+LLT++L+ KM +TA+ +G+EGRI+N+SS I
Sbjct: 121 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 180

Query: 181 HGWFSGDMI---KYLAQISRNKRNYDATRAYALSKLANVLHTHELARRLKQMEANVTVNC 240
           H W   D     K L  ISR    Y+ TRAYA SKLA +LH   L+++LK   ANVT+N 
Sbjct: 181 HNWVKPDCFSFPKLLHPISR----YNGTRAYAQSKLATILHAKALSKQLKDRNANVTINA 240

Query: 241 VHPGIVRTRLTRDREGFITDLVFFMASKLLKTIPQ 273
           VHPGIV+T + R  +G  TD +F +ASKLLK+I Q
Sbjct: 241 VHPGIVKTGIIRAHKGLFTDSLFLIASKLLKSISQ 270

The following BLAST results are available for this feature:

BLAST of Spg029481 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035489.1	7.8e-164	92.01	Short-chain dehydrogenase TIC 32, chloroplastic [Cucurbita argyrosperma subsp. a...	[more]
XP_023534460.1	1.7e-163	92.01	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita pepo ...	[more]
XP_022957741.1	3.0e-163	92.01	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita mosch...	[more]
XP_022995788.1	1.5e-162	91.12	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita maxim...	[more]
KAG6605574.1	1.9e-162	91.74	Short-chain dehydrogenase TIC 32 A, chloroplastic, partial [Cucurbita argyrosper...	[more]

BLAST of Spg029481 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A078IS66	1.0e-70	48.30	Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A0A078ISJ6	2.3e-70	47.99	Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A2RVM0	3.3e-64	44.34	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q6RVV4	3.9e-57	42.67	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
P59837	9.3e-43	40.86	Retinol dehydrogenase 12 OS=Bos taurus OX=9913 GN=RDH12 PE=2 SV=1	[more]

BLAST of Spg029481 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H032	1.4e-163	92.01	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita mos...	[more]
A0A6J1K910	7.1e-163	91.12	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita max...	[more]
A0A6J1H1D2	3.5e-162	91.74	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita mos...	[more]
A0A6J1JZY1	1.7e-161	90.86	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita max...	[more]
A0A5D3C462	2.0e-157	87.87	Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of Spg029481 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24050.1	1.1e-128	70.87	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G64590.1	5.6e-128	73.15	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.1	3.7e-87	54.74	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G02540.1	2.2e-76	49.38	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.2	4.2e-75	56.73	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 208..227 score: 31.71 coord: 115..126 score: 44.39 coord: 171..179 score: 54.94
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 39..56 score: 45.52 coord: 208..227 score: 30.81 coord: 165..181 score: 29.05 coord: 115..126 score: 42.05 coord: 231..248 score: 37.54
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 39..180 e-value: 3.3E-27 score: 95.2
None	No IPR available	GENE3D	3.40.50.720		coord: 35..328 e-value: 4.8E-75 score: 254.3
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 9..328
None	No IPR available	PANTHER	PTHR24320:SF210	MITOCHONDRIAL PYRUVATE CARRIER	coord: 9..328
None	No IPR available	CDD	cd05327	retinol-DH_like_SDR_c_like	coord: 39..316 e-value: 1.0518E-86 score: 260.234
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 39..300

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg029481.1	Spg029481.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity