Spg029448 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg029448 vs. NCBI nr
Match: RVX12042.1 (Splicing factor 3A subunit 2 [Vitis vinifera])

HSP 1 Score: 182.6 bits (462), Expect = 5.4e-42
Identity = 106/326 (32.52%), Postives = 164/326 (50.31%), Query Frame = 0

Query: 36  STPDLEGLLLKDGNIGVWQPGWTTLASIGIKEVVWTGIESLGASEGVLIMWDSNSIICLD 95
           ST + + ++L++    +W     +    G K + W  + + GAS G++I+WDS    C +
Sbjct: 424 STQNPDVVMLQETKREIWDKRLVSSIWKG-KSLDWVALPACGASGGIVILWDSVKFNCSE 483

Query: 96  VVLGVFSALIRINWKGKDLGWVTGIYGPPRPKGRQDFWNELGDLSGLC-GHWCILGDFNV 155
            VLG FS  +++N   ++  W+T +YGP +   R+DFW EL DL GL    WC+ GDFNV
Sbjct: 484 KVLGSFSVTVKLNSDEEESFWLTSVYGPNKAVWREDFWLELQDLHGLTFPRWCVGGDFNV 543

Query: 156 TRSPLEKASRGRVSKSMKSFNEWIEDCNLFDPPLTNGRYTWANNRMH---NRIDRVL--- 215
            R   EK    R++ +M+ F+E+I +  L DPPL N  +TW+N ++     R+DR L   
Sbjct: 544 IRRISEKMGDSRLTVNMRHFDEFIRESGLLDPPLRNAAFTWSNMQVDPICKRLDRFLFSS 603

Query: 216 -----------------NGGHAPSVLRTCGWITRP----------LSPHFLPGGMK---- 275
                               H+P  L T  ++  P          L P F          
Sbjct: 604 EWDSFFSQNIQEALPRWTSDHSPICLETNPFMWGPTPFRFENMWLLHPEFKEKFRDWWQE 663

Query: 276 -----WEGYRLMRKLRSLRSTLKSWNQEVVGDTRVKKQEVLIRIKDIDTLEEDSPLDENL 319
                WEG++ MRKL+ ++S LK WN  V GD R +K+ +L  +  ID +E++  L+  L
Sbjct: 664 CTVEGWEGHKFMRKLKFIKSKLKEWNTRVFGDLRERKKHILTDLGRIDRIEQEGNLNLEL 723

BLAST of Spg029448 vs. NCBI nr
Match: CAN75040.1 (hypothetical protein VITISV_026478 [Vitis vinifera])

HSP 1 Score: 180.6 bits (457), Expect = 2.1e-41
Identity = 94/259 (36.29%), Postives = 139/259 (53.67%), Query Frame = 0

Query: 66  KEVVWTGIESLGASEGVLIMWDSNSIICLDVVLGVFSALIRINWKGKDLGWVTGIYGPPR 125
           K V W  + + GAS G +I+WDS+ + C + V G FS  ++ N   +   W+T +YGP  
Sbjct: 725 KRVEWAALPACGASGGXVILWDSSKLECTEKVXGSFSVTVKFNSGEEGSFWLTSVYGPXN 784

Query: 126 PKGRQDFWNELGDLSGLC-GHWCILGDFNVTRSPLEKASRGRVSKSMKSFNEWIEDCNLF 185
           P  R+DFW EL DL GL    WC+ GDFNV R   EK    R++ +M+ F+E+I +  L 
Sbjct: 785 PLWRKDFWLELQDLFGLTFPRWCVGGDFNVIRRISEKLGETRLTLNMRCFDEFIRESGLI 844

Query: 186 DPPLTNGRYTWANNRMHNRIDRVLNGGHAPSVLRTCGWITRPLSPHFL-----PGGMKWE 245
           DPPL N  +TW+N +              P  LR CG         F        G  WE
Sbjct: 845 DPPLRNAAFTWSNMQA------------XPICLRICGXSILSFKEKFRVWWLEXTGEGWE 904

Query: 246 GYRLMRKLRSLRSTLKSWNQEVVGDTRVKKQEVLIRIKDIDTLEEDSPLDENLKGERFSL 305
           G++ MRKL+ ++S LK WN    GD + +K+ +L  +  ID +E++  L+ +L  ER   
Sbjct: 905 GHKFMRKLKFVKSKLKEWNIMTFGDLKERKKLILTDLSRIDLIEQEGNLNSDLVLERTLK 964

Query: 306 KIEFANLIKKETISWRQRS 319
           + E  +++ KE + WRQ+S
Sbjct: 965 RRELEDVLLKEEVQWRQKS 971

BLAST of Spg029448 vs. NCBI nr
Match: RVW64408.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 180.3 bits (456), Expect = 2.7e-41
Identity = 109/334 (32.63%), Postives = 166/334 (49.70%), Query Frame = 0

Query: 28   RSIPLPIYSTPDLEGLLLKDGNIGVWQPGWTTLASIGIKEVVWTGIESLGASEGVLIMWD 87
            R I     ST + + ++L++     W   + +    G K V W  + + GAS G++I+WD
Sbjct: 752  RRIVRRFLSTQNPDIVMLQETKRETWDRRFVSSVWKG-KRVEWAALPACGASGGIVILWD 811

Query: 88   SNSIICLDVVLGVFSALIRINWKGKDLGWVTGIYGPPRPKGRQDFWNELGDLSGLC-GHW 147
            S+ + C + VLG FS  ++ N   +   W+T +YGP  P  R+DFW EL DL GL    W
Sbjct: 812  SSKLECTEKVLGSFSVTVKFNSGEEGSFWLTSVYGPINPLWRKDFWLELQDLYGLTFPRW 871

Query: 148  CILGDFNVTRSPLEKASRGRVSKSMKSFNEWIEDCNLFDPPLTNGRYTWAN---NRMHNR 207
            C+ GDFNV R   EK    R++ +M+ F+E+I +  L DPPL N  +TW+N   + +  R
Sbjct: 872  CVGGDFNVIRRISEKLGETRLTLNMRCFDEFIRESGLIDPPLRNAAFTWSNMQADPICKR 931

Query: 208  IDRVL--------------------NGGHAPSVLRT--CGWITRP--------LSPHFLP 267
            +DR L                       H+P  L T    W   P        L P F  
Sbjct: 932  LDRFLFSSEWDTFFSQSFQEALPRWTSDHSPICLETNPLKWGPTPFRFENMWLLHPEFKE 991

Query: 268  ---------GGMKWEGYRLMRKLRSLRSTLKSWNQEVVGDTRVKKQEVLIRIKDIDTLEE 319
                      G  WEG++ MRKL+ ++S LK WN    GD + +K+ +L  +  ID +E+
Sbjct: 992  KFRVWWLECTGEGWEGHKFMRKLKFVKSKLKEWNIMTFGDLKERKKLILTDLSRIDLIEQ 1051

BLAST of Spg029448 vs. NCBI nr
Match: RVX15530.1 (putative ribonuclease H protein [Vitis vinifera])

HSP 1 Score: 178.7 bits (452), Expect = 7.8e-41
Identity = 108/334 (32.34%), Postives = 166/334 (49.70%), Query Frame = 0

Query: 28  RSIPLPIYSTPDLEGLLLKDGNIGVWQPGWTTLASIGIKEVVWTGIESLGASEGVLIMWD 87
           R I     ST + + ++L++     W   + +    G K V W  + + GAS G++I+WD
Sbjct: 408 RRIVRRFLSTQNPDIVMLQETKRETWDRRFVSSVWKG-KRVEWAALPACGASGGIVILWD 467

Query: 88  SNSIICLDVVLGVFSALIRINWKGKDLGWVTGIYGPPRPKGRQDFWNELGDLSGLC-GHW 147
           S+   C + VLG FS  ++ N   +   W+T +YGP  P  R+DFW EL DL GL    W
Sbjct: 468 SSKFECTEKVLGSFSVTVKFNSGEEGSFWLTSVYGPINPLWRKDFWLELQDLYGLTFPRW 527

Query: 148 CILGDFNVTRSPLEKASRGRVSKSMKSFNEWIEDCNLFDPPLTNGRYTWAN---NRMHNR 207
           C+ GDFNV R   EK    R++ +M+ F+E+I +  L DPPL N  +TW+N   + +  R
Sbjct: 528 CVGGDFNVIRRISEKLGETRLTFNMRCFDEFIRESGLLDPPLRNAAFTWSNMQADPICKR 587

Query: 208 IDRVL--------------------NGGHAPSVLRT--CGWITRP--------LSPHFLP 267
           +DR L                       H+P  L T    W   P        + P F  
Sbjct: 588 LDRFLFSSEWDTFFSQSFQEALPRWTSDHSPICLETNPLKWGPTPFRFENMWLIHPEFKE 647

Query: 268 ---------GGMKWEGYRLMRKLRSLRSTLKSWNQEVVGDTRVKKQEVLIRIKDIDTLEE 319
                     G  WEG++ MRKL+ ++S LK WN    GD + +K+ +L+ +  ID +E+
Sbjct: 648 KFRVWWQECTGEGWEGHKFMRKLKFVKSKLKEWNIMTFGDLKERKKLILMDLSRIDLIEQ 707

BLAST of Spg029448 vs. NCBI nr
Match: RVW25035.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 177.9 bits (450), Expect = 1.3e-40
Identity = 108/334 (32.34%), Postives = 164/334 (49.10%), Query Frame = 0

Query: 28   RSIPLPIYSTPDLEGLLLKDGNIGVWQPGWTTLASIGIKEVVWTGIESLGASEGVLIMWD 87
            R I     ST + + ++L++     W   + +    G K V W  + + GAS G++I+WD
Sbjct: 1672 RRIVRRFLSTQNPDIVMLQETKRETWDRRFVSSVWTG-KRVEWVALPACGASGGIVILWD 1731

Query: 88   SNSIICLDVVLGVFSALIRINWKGKDLGWVTGIYGPPRPKGRQDFWNELGDLSGLC-GHW 147
            S+   C + VLG FS  ++ N   +   W+T +YGP  P  R+DFW EL DL GL    W
Sbjct: 1732 SSKFECTEKVLGSFSVTVKFNSGEEGSFWLTSVYGPINPLWRKDFWLELQDLYGLTFPRW 1791

Query: 148  CILGDFNVTRSPLEKASRGRVSKSMKSFNEWIEDCNLFDPPLTNGRYTWAN---NRMHNR 207
            C+ GDFNV R   EK    R++ +M+ F+E+I +  L DPPL N  +TW+N   + +  R
Sbjct: 1792 CVGGDFNVIRRISEKLGESRLTFNMRCFDEFIRESGLLDPPLRNAAFTWSNMQADPICKR 1851

Query: 208  IDRVL--------------------NGGHAPSVLRT--CGWITRP--------LSPHFLP 267
            +DR L                       H+P  L T    W   P        L P F  
Sbjct: 1852 LDRFLFSSEWDTFFSQSFQEALPRWTSDHSPICLETNPLKWGPTPFRFENMWLLHPEFKE 1911

Query: 268  ---------GGMKWEGYRLMRKLRSLRSTLKSWNQEVVGDTRVKKQEVLIRIKDIDTLEE 319
                      G  WEG++ MRKL+ ++  LK WN    GD + +K+ +L  +  ID +E+
Sbjct: 1912 KFRVWWQECTGEGWEGHKFMRKLKFVKLKLKKWNIMTFGDLKERKKLILTDLSRIDLIEQ 1971

BLAST of Spg029448 vs. ExPASy TrEMBL
Match: A0A438JSU9 (Splicing factor 3A subunit 2 OS=Vitis vinifera OX=29760 GN=sf3a2 PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 2.6e-42
Identity = 106/326 (32.52%), Postives = 164/326 (50.31%), Query Frame = 0

Query: 36  STPDLEGLLLKDGNIGVWQPGWTTLASIGIKEVVWTGIESLGASEGVLIMWDSNSIICLD 95
           ST + + ++L++    +W     +    G K + W  + + GAS G++I+WDS    C +
Sbjct: 424 STQNPDVVMLQETKREIWDKRLVSSIWKG-KSLDWVALPACGASGGIVILWDSVKFNCSE 483

Query: 96  VVLGVFSALIRINWKGKDLGWVTGIYGPPRPKGRQDFWNELGDLSGLC-GHWCILGDFNV 155
            VLG FS  +++N   ++  W+T +YGP +   R+DFW EL DL GL    WC+ GDFNV
Sbjct: 484 KVLGSFSVTVKLNSDEEESFWLTSVYGPNKAVWREDFWLELQDLHGLTFPRWCVGGDFNV 543

Query: 156 TRSPLEKASRGRVSKSMKSFNEWIEDCNLFDPPLTNGRYTWANNRMH---NRIDRVL--- 215
            R   EK    R++ +M+ F+E+I +  L DPPL N  +TW+N ++     R+DR L   
Sbjct: 544 IRRISEKMGDSRLTVNMRHFDEFIRESGLLDPPLRNAAFTWSNMQVDPICKRLDRFLFSS 603

Query: 216 -----------------NGGHAPSVLRTCGWITRP----------LSPHFLPGGMK---- 275
                               H+P  L T  ++  P          L P F          
Sbjct: 604 EWDSFFSQNIQEALPRWTSDHSPICLETNPFMWGPTPFRFENMWLLHPEFKEKFRDWWQE 663

Query: 276 -----WEGYRLMRKLRSLRSTLKSWNQEVVGDTRVKKQEVLIRIKDIDTLEEDSPLDENL 319
                WEG++ MRKL+ ++S LK WN  V GD R +K+ +L  +  ID +E++  L+  L
Sbjct: 664 CTVEGWEGHKFMRKLKFIKSKLKEWNTRVFGDLRERKKHILTDLGRIDRIEQEGNLNLEL 723

BLAST of Spg029448 vs. ExPASy TrEMBL
Match: M5WJ76 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa015871mg PE=4 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 5.9e-42
Identity = 106/294 (36.05%), Postives = 148/294 (50.34%), Query Frame = 0

Query: 70  WTGIESLGASEGVLIMWDSNSIICLDVVLGVFSALIRINWKGKDLGWVTGIYGPPRPKGR 129
           W    SLG S G+ ++W+S S+  +D ++G FS  IRI        W++GIYGP R + R
Sbjct: 380 WVFSPSLGRSGGIAVLWNSQSVSVIDSMVGEFSVSIRIEENIGTDWWLSGIYGPCRQRER 439

Query: 130 QDFWNELGDLSGLCGH-WCILGDFNVTRSPLEKASRGRVSKSMKSFNEWIEDCNLFDPPL 189
             FW EL DL G CG  WC+ GDFNV R   EK++ GRV+KSM+ FN++I++ NL DP L
Sbjct: 440 NSFWEELADLYGYCGDMWCLGGDFNVVRFSAEKSNEGRVTKSMRDFNDFIQETNLRDPIL 499

Query: 190 TNGRYTWAN---NRMHNRIDRVLNGG--------------------HAPSVLRTCG---- 249
            N  +TW+N   N +  R+DR L  G                    H P  L T      
Sbjct: 500 LNASFTWSNLRENAVCRRLDRFLVSGSWEEHFPHYRHKALPRITSDHCPIELDTSRVKWG 559

Query: 250 ---------WITRP---------LSPHFLPGGMKWEGYRLMRKLRSLRSTLKSWNQEVVG 309
                    W+  P              +PG   WEGY+ M +L+ L+S LK W++E  G
Sbjct: 560 PSPFRFENMWLNHPDFKRKIKLWWGEDQIPG---WEGYKFMTRLKMLKSKLKVWSKEEFG 619

Query: 310 DTRVKKQEVLIRIKDIDTLEEDSPLDENLKGERFSLKIEFANLIKKETISWRQR 318
           D     +E   R+  +D  E    LD  L+ ER +L ++  +L +KE + WRQR
Sbjct: 620 DVERDLREAEARLLVLDQREGTEGLDHLLRSERDNLLLKIGDLAQKEEVKWRQR 670

BLAST of Spg029448 vs. ExPASy TrEMBL
Match: M5VS59 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa016504mg PE=4 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 7.7e-42
Identity = 106/294 (36.05%), Postives = 148/294 (50.34%), Query Frame = 0

Query: 70  WTGIESLGASEGVLIMWDSNSIICLDVVLGVFSALIRINWKGKDLGWVTGIYGPPRPKGR 129
           W    SLG S G+ ++W+S S+  +D ++G FS  IRI        W++GIYGP R + R
Sbjct: 44  WVFSPSLGRSGGIAVLWNSQSVSVIDSMVGEFSVSIRIVENIGTDWWLSGIYGPCRQRER 103

Query: 130 QDFWNELGDLSGLCG-HWCILGDFNVTRSPLEKASRGRVSKSMKSFNEWIEDCNLFDPPL 189
             FW EL DL G CG  WC+ GDFNV R   EK++ GRV+KSM+ FN++I++ NL DP L
Sbjct: 104 NSFWEELADLYGFCGDKWCLGGDFNVVRFSAEKSNEGRVTKSMRDFNDFIQETNLRDPNL 163

Query: 190 TNGRYTWAN---NRMHNRIDRVLNGG--------------------HAPSVLRTCG---- 249
            N  +TW+N   N +  R+DR L  G                    H P  L T      
Sbjct: 164 LNASFTWSNLRENAVCRRLDRFLVSGSWEDHFPHYRHKALPRITSDHCPIELDTSRVKWG 223

Query: 250 ---------WITRP---------LSPHFLPGGMKWEGYRLMRKLRSLRSTLKSWNQEVVG 309
                    W+  P              +PG   WEGY+ M +L+ L+S LK W++E  G
Sbjct: 224 PSPFRFENMWLNHPDFMRKIKLWWGEDQIPG---WEGYKFMTRLKMLKSKLKVWSKEEFG 283

Query: 310 DTRVKKQEVLIRIKDIDTLEEDSPLDENLKGERFSLKIEFANLIKKETISWRQR 318
           D     +E   R+  +D  E    LD  L+ ER +L ++  +L +KE + WRQR
Sbjct: 284 DVERDLREAEARLLVLDQREGTEGLDHLLRSERDNLLLKIGDLAQKEEVKWRQR 334

BLAST of Spg029448 vs. ExPASy TrEMBL
Match: A5BV95 (Reverse transcriptase domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_026478 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.0e-41
Identity = 94/259 (36.29%), Postives = 139/259 (53.67%), Query Frame = 0

Query: 66  KEVVWTGIESLGASEGVLIMWDSNSIICLDVVLGVFSALIRINWKGKDLGWVTGIYGPPR 125
           K V W  + + GAS G +I+WDS+ + C + V G FS  ++ N   +   W+T +YGP  
Sbjct: 725 KRVEWAALPACGASGGXVILWDSSKLECTEKVXGSFSVTVKFNSGEEGSFWLTSVYGPXN 784

Query: 126 PKGRQDFWNELGDLSGLC-GHWCILGDFNVTRSPLEKASRGRVSKSMKSFNEWIEDCNLF 185
           P  R+DFW EL DL GL    WC+ GDFNV R   EK    R++ +M+ F+E+I +  L 
Sbjct: 785 PLWRKDFWLELQDLFGLTFPRWCVGGDFNVIRRISEKLGETRLTLNMRCFDEFIRESGLI 844

Query: 186 DPPLTNGRYTWANNRMHNRIDRVLNGGHAPSVLRTCGWITRPLSPHFL-----PGGMKWE 245
           DPPL N  +TW+N +              P  LR CG         F        G  WE
Sbjct: 845 DPPLRNAAFTWSNMQA------------XPICLRICGXSILSFKEKFRVWWLEXTGEGWE 904

Query: 246 GYRLMRKLRSLRSTLKSWNQEVVGDTRVKKQEVLIRIKDIDTLEEDSPLDENLKGERFSL 305
           G++ MRKL+ ++S LK WN    GD + +K+ +L  +  ID +E++  L+ +L  ER   
Sbjct: 905 GHKFMRKLKFVKSKLKEWNIMTFGDLKERKKLILTDLSRIDLIEQEGNLNSDLVLERTLK 964

Query: 306 KIEFANLIKKETISWRQRS 319
           + E  +++ KE + WRQ+S
Sbjct: 965 RRELEDVLLKEEVQWRQKS 971

BLAST of Spg029448 vs. ExPASy TrEMBL
Match: A0A438FWU5 (LINE-1 retrotransposable element ORF2 protein OS=Vitis vinifera OX=29760 GN=LORF2_70 PE=4 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 1.3e-41
Identity = 109/334 (32.63%), Postives = 166/334 (49.70%), Query Frame = 0

Query: 28   RSIPLPIYSTPDLEGLLLKDGNIGVWQPGWTTLASIGIKEVVWTGIESLGASEGVLIMWD 87
            R I     ST + + ++L++     W   + +    G K V W  + + GAS G++I+WD
Sbjct: 752  RRIVRRFLSTQNPDIVMLQETKRETWDRRFVSSVWKG-KRVEWAALPACGASGGIVILWD 811

Query: 88   SNSIICLDVVLGVFSALIRINWKGKDLGWVTGIYGPPRPKGRQDFWNELGDLSGLC-GHW 147
            S+ + C + VLG FS  ++ N   +   W+T +YGP  P  R+DFW EL DL GL    W
Sbjct: 812  SSKLECTEKVLGSFSVTVKFNSGEEGSFWLTSVYGPINPLWRKDFWLELQDLYGLTFPRW 871

Query: 148  CILGDFNVTRSPLEKASRGRVSKSMKSFNEWIEDCNLFDPPLTNGRYTWAN---NRMHNR 207
            C+ GDFNV R   EK    R++ +M+ F+E+I +  L DPPL N  +TW+N   + +  R
Sbjct: 872  CVGGDFNVIRRISEKLGETRLTLNMRCFDEFIRESGLIDPPLRNAAFTWSNMQADPICKR 931

Query: 208  IDRVL--------------------NGGHAPSVLRT--CGWITRP--------LSPHFLP 267
            +DR L                       H+P  L T    W   P        L P F  
Sbjct: 932  LDRFLFSSEWDTFFSQSFQEALPRWTSDHSPICLETNPLKWGPTPFRFENMWLLHPEFKE 991

Query: 268  ---------GGMKWEGYRLMRKLRSLRSTLKSWNQEVVGDTRVKKQEVLIRIKDIDTLEE 319
                      G  WEG++ MRKL+ ++S LK WN    GD + +K+ +L  +  ID +E+
Sbjct: 992  KFRVWWLECTGEGWEGHKFMRKLKFVKSKLKEWNIMTFGDLKERKKLILTDLSRIDLIEQ 1051