Spg029358 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg029358
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionGMP synthase (glutamine-hydrolyzing), putative / glutamine amidotransferase, putative
Locationscaffold12: 36825631 .. 36829154 (+)
RNA-Seq ExpressionSpg029358
SyntenySpg029358
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACCCCAAAGAATTGAAGTCCGACTTGGTTTTGATTCTGGACTTCGGTTCGCAGTACACCCATCTCATCACCCGCCGGATTCGGAGCCTCTCTGTTTTCTCTCTTTGTATTTCGGGTACTTCTTCGCTTAAGGCCATCACTGATCTCAACCCGCACGTTGTTATTCTATCGGGTGGGCCTCACTCTGTTCATGCTAGCGACTCGCCCAGTTTCGCTTCTGGGTTCGTCGAGTGGGCGGAGGCGAATGGGGTGTTTGTTTTGGGTATTTGTTATGGGCTTCAGCTGATTGTGCAGAGGTTGGGAGGAGTGGTGAAGATTGGAGAGAAGCAGGAGTATGGGAGAATGGAGATTGAGGTGGAGAAGGCTAATGGGTTGTTTGGGACTAAGAAGGTTGGGGACCGGCAAGCTGTTTGGATGAGCCATGGTGATGAGGCGGCGAAACTTCCTGAGGGGTTTGAAGTTGTGGCAAGAAGTCAACAAGGGGCAGTTGCAGCCGTGGAAAATCATTCCAAGAAGTTTTATGGGTTGCAGTATCATCCGGAGGTAATGTTTTTTTTTTTTTGTTTTTTGTTTTTTGTTTTTTTTTTTAAAAACTTTGTGCTCTGTATTGGAATCCTACGTTCTTTCTTTGTACGTTTGGTTGTTTGTTGTTTTTGCATTAATATTCAGTTTCAGGTTGAAAATTTACCACATGTTTGTTTCGGTTCTGGAGAGTTACACCTGAGTTTATCGAACATTGGATTTTGTGTGTGTGATGGCGTGTTAAGAAAAAAAACTGGTTCGAGTAGACCAAACTTGCTTTATGATCATGAAAAGGACGACCTGTTTGAGTTTGACCTAAAATATAATCTTCTGGTGCTGGCCTCTCAAAACGTTTTATATGAGAATTTTTTGTGGTTTCACTGGCTTACTTGTAAACAGATTTCTGCAATTTTTGGTGGCTTATATACTGTTGAAGAACAATGTTTTTATGATGTATAATCATGTGTAGCACCTACTATTGTAGGCCTGAGATGCATAATGCAATTTTTAACTAACGTGACTCATGGTTTTTTTTTGGAACTGATGAAAGGAGAATCTTCATTTATTGTTATCTCCTATGAATTTCTTCATTGGTTATCAAAGAACCTGCCACTGTCAAGTGTCTTGAATGGGAGGGGGATTAGTAGTCATCAAATATTGCTATGTGATGAACTGATGTGATATCATTCGTGGCATAGTCATCAGATAATTGTTAATCTTACGAGAAGTTGAACTATTTGTATTTTGTGTAGGTGACACATTCACCAGAGGGAATGGAAACATTACGTTATTTTCTCTTTGATGTTTGTGGAATTACCGCTAGCTGGAGGATGGAGAATATACTAGATGAAGAACTTAAAATAATCAAAGAAACTGTGGGTCCAGATGATCACGTCATTTGTGCATTATCAGGGGGTGTGGATTCAACAGTTGCTGCAACTCTTGTTCATAAGGCAATTGGGGAAAGGCTTCACTGTGTTTTTGTCGACAATGGTTTATTAAGGTATAGAATTGGACTCTATGTACTGAATGATGAAAGCTTTGCTCAAATTTTACATATATAATTTTACTATAATTCAAAGAGGAAGATCTATCCTCTGTCGATCTATAGAAGTAAACTGTGTGGTACATATTTTGCCCTGTTTTTTCGTTTGTCTTCTCAAAAAAAAAATATTTTGCCCTGTTTTTAAATAAATAAGACTCATCCTGTTGCACAGAAATCATGCAATTTGAGGTCACTGGATTCCATATTTCTCTTAGATAATATCAACATGACTCACCGTGTTGCAGAGTTTTTAATGACACTTTGTGATTATGGCAGGTATAAAGAAAGAGAACGTGTTATGGAAACCTTTGAAAGAGATCTTCATTTGCCTGTTACTTGTGTTGATGCAGTTGATCATTTTCTTAGCAAGCTTAAAGGTGCAGTAGATCCTGAGGTGAAAAGAAAAATAATTGGAAAGGAGTTCATTTCTATATTTGATTCATTTGCCCATGATTTGGAACTTAAAATTGGTAAGAAGCCTTCATACTTGGTCCAAGGAACATTGTATCCTGATGTAATTGAATCTTGTCCACCACCTGGATCTGGAAGTACCCATTCCCACACAATCAAAAGTCATCATAACGTTGGAGGGCTTCCCAAGGACATGAAACTGAAACTTATTGAGCCACTTAAACTCCTCTTTAAAGATGAGGTAATAGTTTTACTGATATGCATATGTCTGTATTATATCACATATTTTTTAATAATCTTTGAATAAAAACTCATAAAGGTACGCCAACTCGGGAGGATATTGAATGTTCCTGATGCGTTTCTAAAGCGTCACCCATTTCCTGGACCTGGTCTTGCTGTACGAGTGTTGGGCGATGTAACAGAAGGCAATGCCTTGGATGTTCTTCGCCAGGTAACTTCAAGTTGTGCCGAGTTCAAACTGTTACATTTCCTGTTTGTGAATAATATTTAGTTTTCTGTAATTATCCTTGCAGGTCGATGAGATTTTTATTCAGTCAATCAAGGATGCTGGGCTTTATGATTCAATATGGCAAGCATTTGCCGTGTTTTTACCTATAAAATCAGTTGGAGTTCAGGGTGATCAAAGGACACATTCTCATGTTGTTGCTCTGAGAGCTGTTACAAGTCAAGATGGAATGACAGCAGATTGGTAAGTTGTCAGCTTGTTCTACTCTTATATGAAACTAAGCATTTTTTGGATAGGATTGTATGAAACTAAGTAGAATTCCTATATACTGCTCCTAGTTTACTTCCAGTATGTCTGCTAAGTTAGAACCCAAAAAACTGATGAAATCGATGCAACGGTCATTTAAAAAACAAACCCAATCTTGATTTTTATTTTATAAAAACTGAAATATTTGTTTGGCATCAAATTTGGCCAAAATCATTTTGAATAGACCCACCTACACTCGTCAAATTGAAGGTAGCTCAACTGGATAAGACATCTATTGTCCAACCAAAAGGTTAAAGGTTCAAATTCCCCGTCTTGCACAATTAACTCAAAAACAAACCCACTAACACTTCTAGCTGCAAGCATCTGGTTTTGCTATTGGTACGGATGACCTGTAGCCTAACATTATGTCCAACTATGTGGAAAATGCATTTGTGATTAAAGACTGGGAGAAACTAATTGAATTCGCATTTTTTAATCTATGATGGTATTTTCCATTCTTGTAGTGGCCTTGTTTCCACACTCCACTCCAGCTCTCATGGACTCATCTCCTAATCCTTATCTAAATATTCTAAATGTTTTAAGATTTTTTGTTTCAACTCCAGTTCTTTACACCATAGTCTATATGTCTGATTAGACCACCTGTGTTTATGTTCCTCTTATTAATTCTGTGGGATATTGATATTCAGGTTTAATTTTGAGCACCAGTTCCTGGATAATGTGTCAAGAAAAATCTGCAATAGTGTTCGGGGCGTAAACAGGGTTGTTCAGGACATTACGTCGAAGCCTCCATCAACAATCGAATGGGAATGA

mRNA sequence

ATGGACCCCAAAGAATTGAAGTCCGACTTGGTTTTGATTCTGGACTTCGGTTCGCAGTACACCCATCTCATCACCCGCCGGATTCGGAGCCTCTCTGTTTTCTCTCTTTGTATTTCGGGTACTTCTTCGCTTAAGGCCATCACTGATCTCAACCCGCACGTTGTTATTCTATCGGGTGGGCCTCACTCTGTTCATGCTAGCGACTCGCCCAGTTTCGCTTCTGGGTTCGTCGAGTGGGCGGAGGCGAATGGGGTGTTTGTTTTGGGTATTTGTTATGGGCTTCAGCTGATTGTGCAGAGGTTGGGAGGAGTGGTGAAGATTGGAGAGAAGCAGGAGTATGGGAGAATGGAGATTGAGGTGGAGAAGGCTAATGGGTTGTTTGGGACTAAGAAGGTTGGGGACCGGCAAGCTGTTTGGATGAGCCATGGTGATGAGGCGGCGAAACTTCCTGAGGGGTTTGAAGTTGTGGCAAGAAGTCAACAAGGGGCAGTTGCAGCCGTGGAAAATCATTCCAAGAAGTTTTATGGGTTGCAGTATCATCCGGAGGTGACACATTCACCAGAGGGAATGGAAACATTACGTTATTTTCTCTTTGATGTTTGTGGAATTACCGCTAGCTGGAGGATGGAGAATATACTAGATGAAGAACTTAAAATAATCAAAGAAACTGTGGGTCCAGATGATCACGTCATTTGTGCATTATCAGGGGGTGTGGATTCAACAGTTGCTGCAACTCTTGTTCATAAGGCAATTGGGGAAAGGCTTCACTGTGTTTTTGTCGACAATGGTTTATTAAGGTATAAAGAAAGAGAACGTGTTATGGAAACCTTTGAAAGAGATCTTCATTTGCCTGTTACTTGTGTTGATGCAGTTGATCATTTTCTTAGCAAGCTTAAAGGTGCAGTAGATCCTGAGGTGAAAAGAAAAATAATTGGAAAGGAGTTCATTTCTATATTTGATTCATTTGCCCATGATTTGGAACTTAAAATTGGTAAGAAGCCTTCATACTTGGTCCAAGGAACATTGTATCCTGATGTAATTGAATCTTGTCCACCACCTGGATCTGGAAGTACCCATTCCCACACAATCAAAAGTCATCATAACGTTGGAGGGCTTCCCAAGGACATGAAACTGAAACTTATTGAGCCACTTAAACTCCTCTTTAAAGATGAGGTACGCCAACTCGGGAGGATATTGAATGTTCCTGATGCGTTTCTAAAGCGTCACCCATTTCCTGGACCTGGTCTTGCTGTACGAGTGTTGGGCGATGTAACAGAAGGCAATGCCTTGGATGTTCTTCGCCAGGTCGATGAGATTTTTATTCAGTCAATCAAGGATGCTGGGCTTTATGATTCAATATGGCAAGCATTTGCCGTGTTTTTACCTATAAAATCAGTTGGAGTTCAGGGTGATCAAAGGACACATTCTCATGTTGTTGCTCTGAGAGCTGTTACAAGTCAAGATGGAATGACAGCAGATTGGTTTAATTTTGAGCACCAGTTCCTGGATAATGTGTCAAGAAAAATCTGCAATAGTGTTCGGGGCGTAAACAGGGTTGTTCAGGACATTACGTCGAAGCCTCCATCAACAATCGAATGGGAATGA

Coding sequence (CDS)

ATGGACCCCAAAGAATTGAAGTCCGACTTGGTTTTGATTCTGGACTTCGGTTCGCAGTACACCCATCTCATCACCCGCCGGATTCGGAGCCTCTCTGTTTTCTCTCTTTGTATTTCGGGTACTTCTTCGCTTAAGGCCATCACTGATCTCAACCCGCACGTTGTTATTCTATCGGGTGGGCCTCACTCTGTTCATGCTAGCGACTCGCCCAGTTTCGCTTCTGGGTTCGTCGAGTGGGCGGAGGCGAATGGGGTGTTTGTTTTGGGTATTTGTTATGGGCTTCAGCTGATTGTGCAGAGGTTGGGAGGAGTGGTGAAGATTGGAGAGAAGCAGGAGTATGGGAGAATGGAGATTGAGGTGGAGAAGGCTAATGGGTTGTTTGGGACTAAGAAGGTTGGGGACCGGCAAGCTGTTTGGATGAGCCATGGTGATGAGGCGGCGAAACTTCCTGAGGGGTTTGAAGTTGTGGCAAGAAGTCAACAAGGGGCAGTTGCAGCCGTGGAAAATCATTCCAAGAAGTTTTATGGGTTGCAGTATCATCCGGAGGTGACACATTCACCAGAGGGAATGGAAACATTACGTTATTTTCTCTTTGATGTTTGTGGAATTACCGCTAGCTGGAGGATGGAGAATATACTAGATGAAGAACTTAAAATAATCAAAGAAACTGTGGGTCCAGATGATCACGTCATTTGTGCATTATCAGGGGGTGTGGATTCAACAGTTGCTGCAACTCTTGTTCATAAGGCAATTGGGGAAAGGCTTCACTGTGTTTTTGTCGACAATGGTTTATTAAGGTATAAAGAAAGAGAACGTGTTATGGAAACCTTTGAAAGAGATCTTCATTTGCCTGTTACTTGTGTTGATGCAGTTGATCATTTTCTTAGCAAGCTTAAAGGTGCAGTAGATCCTGAGGTGAAAAGAAAAATAATTGGAAAGGAGTTCATTTCTATATTTGATTCATTTGCCCATGATTTGGAACTTAAAATTGGTAAGAAGCCTTCATACTTGGTCCAAGGAACATTGTATCCTGATGTAATTGAATCTTGTCCACCACCTGGATCTGGAAGTACCCATTCCCACACAATCAAAAGTCATCATAACGTTGGAGGGCTTCCCAAGGACATGAAACTGAAACTTATTGAGCCACTTAAACTCCTCTTTAAAGATGAGGTACGCCAACTCGGGAGGATATTGAATGTTCCTGATGCGTTTCTAAAGCGTCACCCATTTCCTGGACCTGGTCTTGCTGTACGAGTGTTGGGCGATGTAACAGAAGGCAATGCCTTGGATGTTCTTCGCCAGGTCGATGAGATTTTTATTCAGTCAATCAAGGATGCTGGGCTTTATGATTCAATATGGCAAGCATTTGCCGTGTTTTTACCTATAAAATCAGTTGGAGTTCAGGGTGATCAAAGGACACATTCTCATGTTGTTGCTCTGAGAGCTGTTACAAGTCAAGATGGAATGACAGCAGATTGGTTTAATTTTGAGCACCAGTTCCTGGATAATGTGTCAAGAAAAATCTGCAATAGTGTTCGGGGCGTAAACAGGGTTGTTCAGGACATTACGTCGAAGCCTCCATCAACAATCGAATGGGAATGA

Protein sequence

MDPKELKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGGPHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEVEKANGLFGTKKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYHPEVTHSPEGMETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDSTVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKGAVDPEVKRKIIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRVLGDVTEGNALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVALRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE
Homology
BLAST of Spg029358 vs. NCBI nr
Match: XP_022958390.1 (uncharacterized protein LOC111459626 [Cucurbita moschata])

HSP 1 Score: 1046.2 bits (2704), Expect = 9.7e-302
Identity = 513/534 (96.07%), Postives = 521/534 (97.57%), Query Frame = 0

Query: 1   MDPKELKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGG 60
           MDPKE+KSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTS LKAITDLNP VVILSGG
Sbjct: 1   MDPKEVKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSPLKAITDLNPQVVILSGG 60

Query: 61  PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEV 120
           PHSVHASDSPSFA+GFVEWAEANGVFVLGICYGLQLIVQRLGGVVK+GEKQEYGRMEI V
Sbjct: 61  PHSVHASDSPSFAAGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKVGEKQEYGRMEIVV 120

Query: 121 EKANGLFGTKKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH 180
           EKA GLFGTKKVGD QAVWMSHGDE AKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH
Sbjct: 121 EKAKGLFGTKKVGDSQAVWMSHGDEVAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH 180

Query: 181 PEVTHSPEGMETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDS 240
           PEVTHSPEGMETLRYFLFDVCGITASW+MENILDEE+K+IKETVGPD HVICALSGGVDS
Sbjct: 181 PEVTHSPEGMETLRYFLFDVCGITASWKMENILDEEIKVIKETVGPDAHVICALSGGVDS 240

Query: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKG 300
           TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFE DLHLPVTCVDAVDHFLSKLKG
Sbjct: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFESDLHLPVTCVDAVDHFLSKLKG 300

Query: 301 AVDPEVKRKIIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHS 360
            VDPE KRKIIGKEFISIFDSF HDLELKIGKKPSYLVQGTLYPDVIESCPPPGSG  HS
Sbjct: 301 VVDPEAKRKIIGKEFISIFDSFGHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGRNHS 360

Query: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420
           HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV
Sbjct: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420

Query: 421 LGDVTEGNALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480
           LGDVTEGNAL+VLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA
Sbjct: 421 LGDVTEGNALEVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480

Query: 481 LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 535
           LRAVTSQDGMTADWFNFEHQFLDNV+RKICNSVRGVNRVVQDITSKPPSTIEWE
Sbjct: 481 LRAVTSQDGMTADWFNFEHQFLDNVARKICNSVRGVNRVVQDITSKPPSTIEWE 534

BLAST of Spg029358 vs. NCBI nr
Match: XP_023522242.1 (uncharacterized protein LOC111786132 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023522243.1 uncharacterized protein LOC111786132 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023534331.1 uncharacterized protein LOC111795923 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1045.8 bits (2703), Expect = 1.3e-301
Identity = 512/534 (95.88%), Postives = 522/534 (97.75%), Query Frame = 0

Query: 1   MDPKELKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGG 60
           MDPKE+KSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTS LKAITDLNP VVILSGG
Sbjct: 1   MDPKEVKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSPLKAITDLNPQVVILSGG 60

Query: 61  PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEV 120
           PHSVHASDSPSFA+GFVEWAEANGVFVLGICYGLQLIVQRLGGVVK+GEKQEYGRMEI V
Sbjct: 61  PHSVHASDSPSFAAGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKVGEKQEYGRMEIVV 120

Query: 121 EKANGLFGTKKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH 180
           EKA GLFGTKKVGDRQAVWMSHGDE AKLPEGFEVVARSQQG+VAAVENHSKKF+GLQYH
Sbjct: 121 EKAKGLFGTKKVGDRQAVWMSHGDEVAKLPEGFEVVARSQQGSVAAVENHSKKFFGLQYH 180

Query: 181 PEVTHSPEGMETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDS 240
           PEVTHSPEGMETLRYFLFDVCGITASW+MENILDEE+K+IKETVGPD HVICALSGGVDS
Sbjct: 181 PEVTHSPEGMETLRYFLFDVCGITASWKMENILDEEIKVIKETVGPDAHVICALSGGVDS 240

Query: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKG 300
           TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFE DLHLPVTCVDAVDHFLSKLKG
Sbjct: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFESDLHLPVTCVDAVDHFLSKLKG 300

Query: 301 AVDPEVKRKIIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHS 360
            VDPE KRKIIGKEFISIFDSF HDLELKIGKKPSYLVQGTLYPDVIESCPPPGSG  HS
Sbjct: 301 VVDPEAKRKIIGKEFISIFDSFGHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGRNHS 360

Query: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420
           HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV
Sbjct: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420

Query: 421 LGDVTEGNALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480
           LGDVTEGNAL+VLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA
Sbjct: 421 LGDVTEGNALEVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480

Query: 481 LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 535
           LRAVTSQDGMTADWFNFEHQFLDNV+RKICNSVRGVNRVVQDITSKPPSTIEWE
Sbjct: 481 LRAVTSQDGMTADWFNFEHQFLDNVARKICNSVRGVNRVVQDITSKPPSTIEWE 534

BLAST of Spg029358 vs. NCBI nr
Match: XP_038875856.1 (GMP synthase [glutamine-hydrolyzing] [Benincasa hispida])

HSP 1 Score: 1045.0 bits (2701), Expect = 2.2e-301
Identity = 509/534 (95.32%), Postives = 524/534 (98.13%), Query Frame = 0

Query: 1   MDPKELKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGG 60
           MDPKE+KSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTS LKAITDLNP VVILSGG
Sbjct: 1   MDPKEVKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSPLKAITDLNPRVVILSGG 60

Query: 61  PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEV 120
           PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVK+GEKQEYGRMEIEV
Sbjct: 61  PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKVGEKQEYGRMEIEV 120

Query: 121 EKANGLFGTKKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH 180
           EK NGL+GTKKV DRQ VWMSHGDE AKLPEGFEVVARSQQG+VAAVENHSKKFYGLQYH
Sbjct: 121 EKVNGLYGTKKVRDRQVVWMSHGDEVAKLPEGFEVVARSQQGSVAAVENHSKKFYGLQYH 180

Query: 181 PEVTHSPEGMETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDS 240
           PEVTHSPEGMETLRYFLFDVCGITA+W+MENILDEE+K+IKETVGPDDHVICALSGGVDS
Sbjct: 181 PEVTHSPEGMETLRYFLFDVCGITANWKMENILDEEIKVIKETVGPDDHVICALSGGVDS 240

Query: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKG 300
           TVAATLVHKAIG+RLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVD FLSKLKG
Sbjct: 241 TVAATLVHKAIGDRLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDQFLSKLKG 300

Query: 301 AVDPEVKRKIIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHS 360
            +DPEVKRKIIGKEFISIFD+F H+LELKIGKKPSYLVQGTLYPDVIESCPPPGSG THS
Sbjct: 301 VIDPEVKRKIIGKEFISIFDAFGHELELKIGKKPSYLVQGTLYPDVIESCPPPGSGRTHS 360

Query: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420
           HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVP+AFLKRHPFPGPGLAVRV
Sbjct: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPEAFLKRHPFPGPGLAVRV 420

Query: 421 LGDVTEGNALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480
           LGDVTEGNAL+VLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA
Sbjct: 421 LGDVTEGNALEVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480

Query: 481 LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 535
           LRAVTSQDGMTADWFNFEHQFLDNV+RKICNSVRGVNRVVQDITSKPPSTIEWE
Sbjct: 481 LRAVTSQDGMTADWFNFEHQFLDNVARKICNSVRGVNRVVQDITSKPPSTIEWE 534

BLAST of Spg029358 vs. NCBI nr
Match: XP_022995491.1 (uncharacterized protein LOC111491012 [Cucurbita maxima])

HSP 1 Score: 1044.3 bits (2699), Expect = 3.7e-301
Identity = 511/534 (95.69%), Postives = 521/534 (97.57%), Query Frame = 0

Query: 1   MDPKELKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGG 60
           MDPKE+KSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTS LKAITDLNP VVILSGG
Sbjct: 1   MDPKEVKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSPLKAITDLNPQVVILSGG 60

Query: 61  PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEV 120
           PHSVHASDSPSFA+GFVEWAEANG+FVLGICYGLQLIVQRLGGVVK+GEKQEYGRMEI V
Sbjct: 61  PHSVHASDSPSFAAGFVEWAEANGIFVLGICYGLQLIVQRLGGVVKVGEKQEYGRMEIVV 120

Query: 121 EKANGLFGTKKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH 180
           EK+ GLFGTKKVGDRQAVWMSHGDE AKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH
Sbjct: 121 EKSKGLFGTKKVGDRQAVWMSHGDEVAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH 180

Query: 181 PEVTHSPEGMETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDS 240
           PEVTHSPEGMETLRYFLFDVCGITASW+MENILDEE+K+IKETVGPD HVICALSGGVDS
Sbjct: 181 PEVTHSPEGMETLRYFLFDVCGITASWKMENILDEEIKVIKETVGPDAHVICALSGGVDS 240

Query: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKG 300
           TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFE DLHLPVTCVDAVDHFLSKLKG
Sbjct: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFENDLHLPVTCVDAVDHFLSKLKG 300

Query: 301 AVDPEVKRKIIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHS 360
            VDPE KRKIIGKEFISIFDSF HDLELKIGKKPSYLVQGTLYPDVIESCPPPGSG  HS
Sbjct: 301 VVDPEEKRKIIGKEFISIFDSFGHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGRNHS 360

Query: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420
           HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV
Sbjct: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420

Query: 421 LGDVTEGNALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480
           LGDVTEGNAL+VLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA
Sbjct: 421 LGDVTEGNALEVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480

Query: 481 LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 535
           LRAVTSQDGMTADWFNFEHQFLDNV+RKICNSVR VNRVVQDITSKPPSTIEWE
Sbjct: 481 LRAVTSQDGMTADWFNFEHQFLDNVARKICNSVRAVNRVVQDITSKPPSTIEWE 534

BLAST of Spg029358 vs. NCBI nr
Match: XP_022159721.1 (uncharacterized protein LOC111026060 [Momordica charantia])

HSP 1 Score: 1044.3 bits (2699), Expect = 3.7e-301
Identity = 508/534 (95.13%), Postives = 522/534 (97.75%), Query Frame = 0

Query: 1   MDPKELKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGG 60
           MDPK +KSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTS LKAITDLNP VVILSGG
Sbjct: 1   MDPKAVKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSPLKAITDLNPQVVILSGG 60

Query: 61  PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEV 120
           PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVK+GEKQEYGRMEIEV
Sbjct: 61  PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKVGEKQEYGRMEIEV 120

Query: 121 EKANGLFGTKKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH 180
           EKANGLFGTKK GDRQ VWMSHGDE A+LP+GF+VVARSQQG+VAAVENHSKKF+GLQYH
Sbjct: 121 EKANGLFGTKKAGDRQVVWMSHGDEVAELPDGFQVVARSQQGSVAAVENHSKKFFGLQYH 180

Query: 181 PEVTHSPEGMETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDS 240
           PEVTHSPEGMETLRYFL DVCG+TASW+MENILDEE+K IKETVGPDDHVICALSGGVDS
Sbjct: 181 PEVTHSPEGMETLRYFLLDVCGVTASWKMENILDEEMKAIKETVGPDDHVICALSGGVDS 240

Query: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKG 300
           TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDA DHFLS+LKG
Sbjct: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAADHFLSRLKG 300

Query: 301 AVDPEVKRKIIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHS 360
            +DPE+KRKIIGKEFISIFDSFAHDLELK GKKPSYLVQGTLYPDVIESCPPPGSG THS
Sbjct: 301 VIDPEMKRKIIGKEFISIFDSFAHDLELKFGKKPSYLVQGTLYPDVIESCPPPGSGRTHS 360

Query: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420
           HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVP+AFLKRHPFPGPGLAVRV
Sbjct: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPEAFLKRHPFPGPGLAVRV 420

Query: 421 LGDVTEGNALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480
           LGDVTEGNALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA
Sbjct: 421 LGDVTEGNALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480

Query: 481 LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 535
           LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE
Sbjct: 481 LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 534

BLAST of Spg029358 vs. ExPASy Swiss-Prot
Match: O66601 (GMP synthase [glutamine-hydrolyzing] OS=Aquifex aeolicus (strain VF5) OX=224324 GN=guaA PE=3 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 1.6e-153
Identity = 283/525 (53.90%), Postives = 362/525 (68.95%), Query Frame = 0

Query: 11  VLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGGPHSVHASDSP 70
           +L+++FGSQY  LI RR+R L V+S  +   + ++ I   NP+ +I SGGP SV+A  +P
Sbjct: 6   ILVVNFGSQYVQLIARRVRELGVYSEIVHWDTPVEEIKKKNPYGIIFSGGPASVYAEGAP 65

Query: 71  SFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEVEKANGLF-GT 130
                  E     GV +LGICYGLQ+I  +LGG V   EKQEYGR  + + K + +F G 
Sbjct: 66  LPDKRIYEL----GVPILGICYGLQVITHQLGGKVVRSEKQEYGRARLRIIKEDVIFEGI 125

Query: 131 KKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYHPEVTHSPEG 190
            K  D   VWMSH D+  +LPEGFEV+A S+    A + N  KK YG Q+HPEVTH+  G
Sbjct: 126 PKESD---VWMSHADKVVELPEGFEVLAVSENSPYAVIANREKKIYGFQFHPEVTHTVFG 185

Query: 191 METLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDSTVAATLVHK 250
            E L  F++ VC    +W M + + E+++ I++TVG D  VI ALSGGVDSTVAA L H+
Sbjct: 186 KEMLANFIYGVCKAEKNWEMGDFIHEKIEEIRKTVG-DAKVIAALSGGVDSTVAAVLTHR 245

Query: 251 AIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKGAVDPEVKRK 310
           AIG++LHC F+D+GLLRYKERE V +   R L LP+T VDA + FL KLKG  DPE KRK
Sbjct: 246 AIGDKLHCFFIDHGLLRYKEREEVEKNL-RSLGLPLTVVDASEEFLEKLKGVEDPEEKRK 305

Query: 311 IIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHSHTIKSHHNV 370
           IIG+ FI +F+  A  +E        +L+QGTLYPDV+ES    GS       IK+HHNV
Sbjct: 306 IIGRTFIEVFEREAKKIE-----GAEFLLQGTLYPDVVESAGIKGSAK-----IKTHHNV 365

Query: 371 GGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRVLGDVTEGNA 430
           GGLP+ M LKL+EP + LFKDEVR++G++L VP+  L+RHPFPGPGLA+R++G+V + + 
Sbjct: 366 GGLPERMNLKLLEPFRELFKDEVRKIGKLLGVPEEILRRHPFPGPGLAIRIIGEVNKKD- 425

Query: 431 LDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVALRAVTSQDG 490
           L++LR+ D IFIQ +K  GLYD +WQAFAV LP+KSVGV GD RT+  VVALRAV S DG
Sbjct: 426 LEILRKADYIFIQELKKEGLYDRVWQAFAVLLPVKSVGVMGDVRTYEKVVALRAVESVDG 485

Query: 491 MTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 535
           MTADW    + FLD V R+I N V GVNRVV DI+SKPPSTIEWE
Sbjct: 486 MTADWARLPYDFLDRVMRRIINEVEGVNRVVYDISSKPPSTIEWE 510

BLAST of Spg029358 vs. ExPASy Swiss-Prot
Match: Q3AD70 (GMP synthase [glutamine-hydrolyzing] OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901) OX=246194 GN=guaA PE=3 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-148
Identity = 267/524 (50.95%), Postives = 361/524 (68.89%), Query Frame = 0

Query: 11  VLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGGPHSVHASDSP 70
           V++LDFG QY+ LI RRIR L V+   +   + L+ I   NP  ++ SGGP SV+   +P
Sbjct: 5   VIVLDFGGQYSQLIARRIRELKVYCEMLPYNTPLEKIVQENPGGIVFSGGPSSVYGEGAP 64

Query: 71  SFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEVEKANGLFGTK 130
           +             + +LGICYG+QL+  +LGG+V+  E +EYG+  + +   + LF   
Sbjct: 65  TVDPEIYRL----NIPILGICYGMQLMAHQLGGIVRPAEGREYGKTPLFILNRDRLFA-- 124

Query: 131 KVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYHPEVTHSPEGM 190
            + + +  WMSHGD  AK PEGF V A+++   +AA+EN  +  Y +Q+HPEV H+P+G 
Sbjct: 125 GLNETEICWMSHGDFVAKAPEGFLVTAKTEYTPIAAMENRERNLYAVQFHPEVVHTPKGK 184

Query: 191 ETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDSTVAATLVHKA 250
           E L+ FL+++CG+T  W ME+   + ++ IKE VG ++ V+CALSGGVDS+VAA LVHKA
Sbjct: 185 EILKNFLYEICGLTPDWTMESFAQKAIREIKEQVG-EEKVVCALSGGVDSSVAAVLVHKA 244

Query: 251 IGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKGAVDPEVKRKI 310
           IG+ L C+FVD+GLLR  E E V+ TF+    + +  VDA + FL+KLKG  DPE KRKI
Sbjct: 245 IGDNLTCIFVDHGLLRKGEAEEVVRTFKEQFQMNLVFVDAKEQFLAKLKGVRDPEQKRKI 304

Query: 311 IGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHSHTIKSHHNVG 370
           IG EFI +F+  A     K+G    +LVQGTLYPDV+E      SG+  + TIKSHHNVG
Sbjct: 305 IGHEFIRVFEEEA----AKLG-DIRFLVQGTLYPDVVE------SGTATAATIKSHHNVG 364

Query: 371 GLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRVLGDVTEGNAL 430
           GLP+DMK +LIEPLK LFKDEVR+LG  L +P++ + RHPFPGPGLAVRVLG++TE   L
Sbjct: 365 GLPEDMKFQLIEPLKWLFKDEVRELGLELGLPESIVWRHPFPGPGLAVRVLGEITE-EKL 424

Query: 431 DVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVALRAVTSQDGM 490
            +LR+ D IFI  +K +G Y   WQAFAV   ++SVGV GD+RT+++ +ALRAVTS+DGM
Sbjct: 425 AILREADYIFIDELKKSGWYRKTWQAFAVLPNLQSVGVMGDERTYAYTIALRAVTSEDGM 484

Query: 491 TADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 535
           TADW    ++ L+ +S +I   V+GVNRVV DITSKPP+TIEWE
Sbjct: 485 TADWVRLPYELLEKISARIVGEVKGVNRVVYDITSKPPATIEWE 509

BLAST of Spg029358 vs. ExPASy Swiss-Prot
Match: C0QYF1 (GMP synthase [glutamine-hydrolyzing] OS=Brachyspira hyodysenteriae (strain ATCC 49526 / WA1) OX=565034 GN=guaA PE=3 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 9.8e-148
Identity = 266/526 (50.57%), Postives = 356/526 (67.68%), Query Frame = 0

Query: 9   DLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGGPHSVHASD 68
           D VLILDFGSQ+T LITRRIR L+V+S       S+  I +  P  +ILSGGP SV+  D
Sbjct: 6   DKVLILDFGSQFTQLITRRIRELNVYSEIHPFHVSIDFIKEFKPKAIILSGGPSSVYEED 65

Query: 69  SPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEVEKANGLFG 128
           +P       E     G+ +LGICYG+Q+IV  +GG V+  +K+EYG+ EIE+     +F 
Sbjct: 66  APKVDKQLFEL----GMPILGICYGMQIIVYSMGGKVESADKREYGKAEIEITNHESIF- 125

Query: 129 TKKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYHPEVTHSPE 188
            K  G    VWMSHGD    +PEGFE++A++    +AA+EN  K  Y +Q+HPEV H+  
Sbjct: 126 -KSFGKSNIVWMSHGDSIKSIPEGFELIAKTPNTELAAIENKQKNIYAIQFHPEVVHTEN 185

Query: 189 GMETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDSTVAATLVH 248
           G++ +  FLF++C    +W M + ++ E+K I+ETVG D +VI  LSGGVDS+VAA L+ 
Sbjct: 186 GIKIIENFLFNICKCERNWNMGSFIEYEIKRIRETVG-DKNVILGLSGGVDSSVAAVLIE 245

Query: 249 KAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKGAVDPEVKR 308
           KAIG++L C+FV+NGLLR  E ++V+E F  + ++ +  VDA   FL KL G  DPE KR
Sbjct: 246 KAIGKQLKCIFVNNGLLRKDEDKKVVEVFRDNFNIDLIYVDASKRFLDKLAGVTDPEQKR 305

Query: 309 KIIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHSHTIKSHHN 368
           K+IG EF+S+F+  A  +E        +L QGTLYPDVIES    GS    S  IKSHHN
Sbjct: 306 KVIGHEFVSVFNDEAKKIE-----NVGFLAQGTLYPDVIESVSLRGS----SAVIKSHHN 365

Query: 369 VGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRVLGDVTEGN 428
           VGGLPKDMK +L+EP + LFKDEVR++G  L +P+  + R PFPGPGLAVR+LGD+TE  
Sbjct: 366 VGGLPKDMKFELLEPFRELFKDEVREIGLELKLPEDIVYRQPFPGPGLAVRILGDITE-E 425

Query: 429 ALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVALRAVTSQD 488
            + +L++ D+I +  IK AGLY  +WQ+FA+ LP+KSVGV GD RT+  V A+RAV S D
Sbjct: 426 RVKILQEADDIVVSEIKKAGLYRKLWQSFAILLPVKSVGVMGDGRTYEQVCAVRAVESVD 485

Query: 489 GMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 535
            MTADW   ++  L  +S +I N V+G+NRVV DI+SKPP+TIEWE
Sbjct: 486 AMTADWAKIDYNVLGIISNRIINEVKGINRVVYDISSKPPATIEWE 514

BLAST of Spg029358 vs. ExPASy Swiss-Prot
Match: B6JHM4 (GMP synthase [glutamine-hydrolyzing] OS=Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) OX=504832 GN=guaA PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.7e-147
Identity = 278/527 (52.75%), Postives = 353/527 (66.98%), Query Frame = 0

Query: 9   DLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGGPHSVHASD 68
           D +LI+DFGSQ T LI RR+R   V+S  +    + +A T + P  VILSGGP SV  + 
Sbjct: 19  DKILIVDFGSQVTQLIARRVREEGVYSEIVPFQKAEQAFTAMQPKAVILSGGPASVLEAG 78

Query: 69  SPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEVEKANGLF- 128
           +PS            GV VLGICYG Q + Q+LGG+V+ G  +E+GR  IE+     LF 
Sbjct: 79  APSAPMSIF----TAGVPVLGICYGEQTMAQQLGGMVEGGHHREFGRAAIEITDDCALFE 138

Query: 129 GTKKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYHPEVTHSP 188
           G  K G++  VWMSHGD   KLPEGF  VAR+    +A + + ++KFY +Q+HPEV H+P
Sbjct: 139 GVWKKGEQHDVWMSHGDRVTKLPEGFRGVARAAGSPIAIIADDTRKFYAMQFHPEVVHTP 198

Query: 189 EGMETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDSTVAATLV 248
           +G + +R F+  V G+   W M    +E ++ I+  VG    VIC LSGGVDS VAA L+
Sbjct: 199 DGAKLIRNFVRKVAGLKGDWTMRAFREEAIEKIRAQVG-QGRVICGLSGGVDSAVAAVLI 258

Query: 249 HKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKGAVDPEVK 308
           H+AIG++L CVFVD+GLLR  E E V+  F    ++P+  VDA   FL +L G  DPE+K
Sbjct: 259 HEAIGDQLTCVFVDHGLLRMAEAETVVSLFRDHYNIPLVHVDASKTFLGELAGVTDPELK 318

Query: 309 RKIIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHSHTIKSHH 368
           RK IG+ FI +FD  A     KIG    +L QGTLYPDVIES    G  S    TIKSHH
Sbjct: 319 RKTIGRLFIDVFDEEAK----KIG-GADFLAQGTLYPDVIESVSFTGGPSV---TIKSHH 378

Query: 369 NVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRVLGDVTEG 428
           NVGGLP  MK+KL+EPL+ LFKDEVR LGR L +PD F+ RHPFPGPGLA+R  G++T  
Sbjct: 379 NVGGLPARMKMKLVEPLRELFKDEVRALGRELGLPDVFVGRHPFPGPGLAIRCPGEITP- 438

Query: 429 NALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVALRAVTSQ 488
             LD+LRQ D I+I+ I+ AGLYD+IWQAFAV LP+K+VGV GD RT+  VV LRAVTS 
Sbjct: 439 EKLDILRQADAIYIEEIRRAGLYDTIWQAFAVLLPVKTVGVMGDGRTYEFVVGLRAVTST 498

Query: 489 DGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 535
           DGMTAD++ F+ +FL   + +I N V+GVNRVV D+TSKPP TIEWE
Sbjct: 499 DGMTADFYPFDMRFLGETATRIINEVKGVNRVVYDVTSKPPGTIEWE 531

BLAST of Spg029358 vs. ExPASy Swiss-Prot
Match: Q1QKC3 (GMP synthase [glutamine-hydrolyzing] OS=Nitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14) OX=323097 GN=guaA PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.1e-146
Identity = 270/527 (51.23%), Postives = 350/527 (66.41%), Query Frame = 0

Query: 9   DLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGGPHSVHASD 68
           D +LI+DFGSQ T LI RRIR   V+S  +    +  A  ++ P  VILSGGP SV   D
Sbjct: 23  DKILIVDFGSQVTQLIARRIREEGVYSEIVPFQKAEAAFLEMKPKAVILSGGPASVLDKD 82

Query: 69  SPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEVEKANGLF- 128
           +P+     ++     GV VLGICYG Q + Q+LGG V+ G  +E+GR  IEV     LF 
Sbjct: 83  APAAPMSILK----AGVPVLGICYGEQTMAQQLGGTVEAGHHREFGRAAIEVTDTCALFE 142

Query: 129 GTKKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYHPEVTHSP 188
           G  + G    VWMSHGD   KLP+GF  VA++Q   +A + + +++FY +Q+HPEV H+P
Sbjct: 143 GVWEKGGHYNVWMSHGDRVTKLPDGFRAVAKAQGSPIAVIADDARRFYAMQFHPEVVHTP 202

Query: 189 EGMETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDSTVAATLV 248
           +G + LR F+  V G+T  W M    +E ++ I+  VG    VIC LSGGVDS+VAA L+
Sbjct: 203 DGAKLLRNFVRKVAGLTGDWTMRAFREEAIEKIRAQVG-KGRVICGLSGGVDSSVAAILI 262

Query: 249 HKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKGAVDPEVK 308
           H+AIGE+L CVFVD+G+LR  E + V+E F    ++P+  VDA   FL +L G  DPE+K
Sbjct: 263 HEAIGEQLTCVFVDHGMLRKDEGKTVVELFRHHYNIPLVHVDASKQFLGELAGVTDPEMK 322

Query: 309 RKIIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHSHTIKSHH 368
           RK IG+ FI +F++ A  +         +L QGTLYPDVIES    G  S    TIKSHH
Sbjct: 323 RKTIGRLFIDVFEAEAKKIAALGKGSAEFLAQGTLYPDVIESVSFTGGPSV---TIKSHH 382

Query: 369 NVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRVLGDVTEG 428
           NVGGLP  M +KL+EPL+ LFKDEVR LGR L +P+ F+ RHPFPGPGLA+R  G++T  
Sbjct: 383 NVGGLPDRMNMKLVEPLRELFKDEVRALGRELGLPEIFVGRHPFPGPGLAIRCPGEITR- 442

Query: 429 NALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVALRAVTSQ 488
             LD+LR+ D ++I  I+ AGLYD IWQAFAV LP+K+VGV GD RT+ +VV LRAVTS 
Sbjct: 443 EKLDILREADAVYIDQIRKAGLYDKIWQAFAVLLPVKTVGVMGDGRTYEYVVGLRAVTST 502

Query: 489 DGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 535
           DGMTAD++ F+  FL   +  I N V+GVNRVV D+TSKPP TIEWE
Sbjct: 503 DGMTADFYAFDATFLGATATSIINEVKGVNRVVYDVTSKPPGTIEWE 540

BLAST of Spg029358 vs. ExPASy TrEMBL
Match: A0A6J1H3B6 (Glutamine amidotransferase OS=Cucurbita moschata OX=3662 GN=LOC111459626 PE=3 SV=1)

HSP 1 Score: 1046.2 bits (2704), Expect = 4.7e-302
Identity = 513/534 (96.07%), Postives = 521/534 (97.57%), Query Frame = 0

Query: 1   MDPKELKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGG 60
           MDPKE+KSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTS LKAITDLNP VVILSGG
Sbjct: 1   MDPKEVKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSPLKAITDLNPQVVILSGG 60

Query: 61  PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEV 120
           PHSVHASDSPSFA+GFVEWAEANGVFVLGICYGLQLIVQRLGGVVK+GEKQEYGRMEI V
Sbjct: 61  PHSVHASDSPSFAAGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKVGEKQEYGRMEIVV 120

Query: 121 EKANGLFGTKKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH 180
           EKA GLFGTKKVGD QAVWMSHGDE AKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH
Sbjct: 121 EKAKGLFGTKKVGDSQAVWMSHGDEVAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH 180

Query: 181 PEVTHSPEGMETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDS 240
           PEVTHSPEGMETLRYFLFDVCGITASW+MENILDEE+K+IKETVGPD HVICALSGGVDS
Sbjct: 181 PEVTHSPEGMETLRYFLFDVCGITASWKMENILDEEIKVIKETVGPDAHVICALSGGVDS 240

Query: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKG 300
           TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFE DLHLPVTCVDAVDHFLSKLKG
Sbjct: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFESDLHLPVTCVDAVDHFLSKLKG 300

Query: 301 AVDPEVKRKIIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHS 360
            VDPE KRKIIGKEFISIFDSF HDLELKIGKKPSYLVQGTLYPDVIESCPPPGSG  HS
Sbjct: 301 VVDPEAKRKIIGKEFISIFDSFGHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGRNHS 360

Query: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420
           HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV
Sbjct: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420

Query: 421 LGDVTEGNALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480
           LGDVTEGNAL+VLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA
Sbjct: 421 LGDVTEGNALEVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480

Query: 481 LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 535
           LRAVTSQDGMTADWFNFEHQFLDNV+RKICNSVRGVNRVVQDITSKPPSTIEWE
Sbjct: 481 LRAVTSQDGMTADWFNFEHQFLDNVARKICNSVRGVNRVVQDITSKPPSTIEWE 534

BLAST of Spg029358 vs. ExPASy TrEMBL
Match: A0A6J1K223 (Glutamine amidotransferase OS=Cucurbita maxima OX=3661 GN=LOC111491012 PE=3 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 1.8e-301
Identity = 511/534 (95.69%), Postives = 521/534 (97.57%), Query Frame = 0

Query: 1   MDPKELKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGG 60
           MDPKE+KSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTS LKAITDLNP VVILSGG
Sbjct: 1   MDPKEVKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSPLKAITDLNPQVVILSGG 60

Query: 61  PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEV 120
           PHSVHASDSPSFA+GFVEWAEANG+FVLGICYGLQLIVQRLGGVVK+GEKQEYGRMEI V
Sbjct: 61  PHSVHASDSPSFAAGFVEWAEANGIFVLGICYGLQLIVQRLGGVVKVGEKQEYGRMEIVV 120

Query: 121 EKANGLFGTKKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH 180
           EK+ GLFGTKKVGDRQAVWMSHGDE AKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH
Sbjct: 121 EKSKGLFGTKKVGDRQAVWMSHGDEVAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH 180

Query: 181 PEVTHSPEGMETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDS 240
           PEVTHSPEGMETLRYFLFDVCGITASW+MENILDEE+K+IKETVGPD HVICALSGGVDS
Sbjct: 181 PEVTHSPEGMETLRYFLFDVCGITASWKMENILDEEIKVIKETVGPDAHVICALSGGVDS 240

Query: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKG 300
           TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFE DLHLPVTCVDAVDHFLSKLKG
Sbjct: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFENDLHLPVTCVDAVDHFLSKLKG 300

Query: 301 AVDPEVKRKIIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHS 360
            VDPE KRKIIGKEFISIFDSF HDLELKIGKKPSYLVQGTLYPDVIESCPPPGSG  HS
Sbjct: 301 VVDPEEKRKIIGKEFISIFDSFGHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGRNHS 360

Query: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420
           HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV
Sbjct: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420

Query: 421 LGDVTEGNALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480
           LGDVTEGNAL+VLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA
Sbjct: 421 LGDVTEGNALEVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480

Query: 481 LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 535
           LRAVTSQDGMTADWFNFEHQFLDNV+RKICNSVR VNRVVQDITSKPPSTIEWE
Sbjct: 481 LRAVTSQDGMTADWFNFEHQFLDNVARKICNSVRAVNRVVQDITSKPPSTIEWE 534

BLAST of Spg029358 vs. ExPASy TrEMBL
Match: A0A6J1E4S4 (Glutamine amidotransferase OS=Momordica charantia OX=3673 GN=LOC111026060 PE=3 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 1.8e-301
Identity = 508/534 (95.13%), Postives = 522/534 (97.75%), Query Frame = 0

Query: 1   MDPKELKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGG 60
           MDPK +KSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTS LKAITDLNP VVILSGG
Sbjct: 1   MDPKAVKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSPLKAITDLNPQVVILSGG 60

Query: 61  PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEV 120
           PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVK+GEKQEYGRMEIEV
Sbjct: 61  PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKVGEKQEYGRMEIEV 120

Query: 121 EKANGLFGTKKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH 180
           EKANGLFGTKK GDRQ VWMSHGDE A+LP+GF+VVARSQQG+VAAVENHSKKF+GLQYH
Sbjct: 121 EKANGLFGTKKAGDRQVVWMSHGDEVAELPDGFQVVARSQQGSVAAVENHSKKFFGLQYH 180

Query: 181 PEVTHSPEGMETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDS 240
           PEVTHSPEGMETLRYFL DVCG+TASW+MENILDEE+K IKETVGPDDHVICALSGGVDS
Sbjct: 181 PEVTHSPEGMETLRYFLLDVCGVTASWKMENILDEEMKAIKETVGPDDHVICALSGGVDS 240

Query: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKG 300
           TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDA DHFLS+LKG
Sbjct: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAADHFLSRLKG 300

Query: 301 AVDPEVKRKIIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHS 360
            +DPE+KRKIIGKEFISIFDSFAHDLELK GKKPSYLVQGTLYPDVIESCPPPGSG THS
Sbjct: 301 VIDPEMKRKIIGKEFISIFDSFAHDLELKFGKKPSYLVQGTLYPDVIESCPPPGSGRTHS 360

Query: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420
           HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVP+AFLKRHPFPGPGLAVRV
Sbjct: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPEAFLKRHPFPGPGLAVRV 420

Query: 421 LGDVTEGNALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480
           LGDVTEGNALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA
Sbjct: 421 LGDVTEGNALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480

Query: 481 LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 535
           LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE
Sbjct: 481 LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 534

BLAST of Spg029358 vs. ExPASy TrEMBL
Match: A0A0A0KKJ0 (Glutamine amidotransferase OS=Cucumis sativus OX=3659 GN=Csa_5G153080 PE=3 SV=1)

HSP 1 Score: 1043.9 bits (2698), Expect = 2.3e-301
Identity = 508/534 (95.13%), Postives = 524/534 (98.13%), Query Frame = 0

Query: 1   MDPKELKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGG 60
           MDPKE+KSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNP VVILSGG
Sbjct: 1   MDPKEVKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPQVVILSGG 60

Query: 61  PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEV 120
           PHSVHA DSPSFASGFVEWAEANGV+VLGICYGLQLIVQRLGGVV++GEKQEYGRMEIEV
Sbjct: 61  PHSVHACDSPSFASGFVEWAEANGVYVLGICYGLQLIVQRLGGVVEVGEKQEYGRMEIEV 120

Query: 121 EKANGLFGTKKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH 180
           EKANGL+GTK+VGDRQ VWMSHGDE AKLP+GFEVVARSQQGAVAAVENHSK+FYGLQYH
Sbjct: 121 EKANGLYGTKRVGDRQVVWMSHGDEVAKLPQGFEVVARSQQGAVAAVENHSKRFYGLQYH 180

Query: 181 PEVTHSPEGMETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDS 240
           PEVTHSPEGMETLRYFLFDVCGITA+W+MEN+LDEE+ IIKETVGPDDHVICALSGGVDS
Sbjct: 181 PEVTHSPEGMETLRYFLFDVCGITANWKMENLLDEEINIIKETVGPDDHVICALSGGVDS 240

Query: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKG 300
           TVAATLVHKAIG+RLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVD FLSKLKG
Sbjct: 241 TVAATLVHKAIGDRLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDQFLSKLKG 300

Query: 301 AVDPEVKRKIIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHS 360
             DPEVKRKIIGKEFISIFDSF HDLELKIGKKPSYLVQGTLYPDVIESCPPPGSG THS
Sbjct: 301 VTDPEVKRKIIGKEFISIFDSFGHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGKTHS 360

Query: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420
           HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLG+ILNVP+AFLKRHPFPGPGLAVRV
Sbjct: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGKILNVPNAFLKRHPFPGPGLAVRV 420

Query: 421 LGDVTEGNALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480
           LGDVTEGNAL+VLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA
Sbjct: 421 LGDVTEGNALEVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480

Query: 481 LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 535
           LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE
Sbjct: 481 LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 534

BLAST of Spg029358 vs. ExPASy TrEMBL
Match: A0A5A7THI7 (Glutamine amidotransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002530 PE=3 SV=1)

HSP 1 Score: 1040.4 bits (2689), Expect = 2.6e-300
Identity = 507/534 (94.94%), Postives = 523/534 (97.94%), Query Frame = 0

Query: 1   MDPKELKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGG 60
           MDPKE+KSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNP VVILSGG
Sbjct: 1   MDPKEVKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPQVVILSGG 60

Query: 61  PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEV 120
           PHSVHA DSPSFASGFVEWAEANGV+VLGICYGLQLIVQRLGGVV++GEKQEYGRMEIEV
Sbjct: 61  PHSVHACDSPSFASGFVEWAEANGVYVLGICYGLQLIVQRLGGVVEVGEKQEYGRMEIEV 120

Query: 121 EKANGLFGTKKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH 180
           EKANGL+GTK+VGDRQ VWMSHGDE AKLP+GFEVVARSQQGAVAAVEN SK+FYGLQYH
Sbjct: 121 EKANGLYGTKRVGDRQVVWMSHGDEVAKLPQGFEVVARSQQGAVAAVENRSKRFYGLQYH 180

Query: 181 PEVTHSPEGMETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDS 240
           PEVTHSPEGMETLRYFLFDVCGI A+W+MEN+LDEE+KIIKETVGPDDHVICALSGGVDS
Sbjct: 181 PEVTHSPEGMETLRYFLFDVCGIAANWKMENLLDEEIKIIKETVGPDDHVICALSGGVDS 240

Query: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKG 300
           TVAATLVHKAIG+RLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVD FLSKLKG
Sbjct: 241 TVAATLVHKAIGDRLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDQFLSKLKG 300

Query: 301 AVDPEVKRKIIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHS 360
             DPEVKRKIIGKEFISIFDSF HDLELKIGKKPSYLVQGTLYPDVIESCPPPGSG THS
Sbjct: 301 VTDPEVKRKIIGKEFISIFDSFGHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGRTHS 360

Query: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420
           HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLG+ILNVP+AFLKRHPFPGPGLAVRV
Sbjct: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGKILNVPNAFLKRHPFPGPGLAVRV 420

Query: 421 LGDVTEGNALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480
           LGDVTEGNAL+VLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA
Sbjct: 421 LGDVTEGNALEVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480

Query: 481 LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 535
           LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE
Sbjct: 481 LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 534

BLAST of Spg029358 vs. TAIR 10
Match: AT1G63660.1 (GMP synthase (glutamine-hydrolyzing), putative / glutamine amidotransferase, putative )

HSP 1 Score: 907.5 bits (2344), Expect = 5.0e-264
Identity = 434/534 (81.27%), Postives = 490/534 (91.76%), Query Frame = 0

Query: 1   MDPKELKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGG 60
           M+   +K D VLILD+GSQYTHLITRRIRSL+VFSL ISGTSSLK+IT  NP VVILSGG
Sbjct: 1   METPTMKPDTVLILDYGSQYTHLITRRIRSLNVFSLVISGTSSLKSITSYNPRVVILSGG 60

Query: 61  PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEV 120
           PHSVHA D+PSF  GF+EWAE+NGV VLGICYGLQLIVQ+LGGVV  GE +EYG+MEIEV
Sbjct: 61  PHSVHALDAPSFPEGFIEWAESNGVSVLGICYGLQLIVQKLGGVVVEGESKEYGKMEIEV 120

Query: 121 EKANGLFGTKKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH 180
           +  + +FG++  G++Q VWMSHGDEA KLPEGFEVVA+S QGAVAA+E+  KK YGLQYH
Sbjct: 121 KGKSEIFGSESGGEKQMVWMSHGDEAVKLPEGFEVVAQSAQGAVAALESRKKKIYGLQYH 180

Query: 181 PEVTHSPEGMETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDS 240
           PEVTHSP+GMETLR+FLFDVCG++A W+ME++++EE+K+I +TV  D+HVICALSGGVDS
Sbjct: 181 PEVTHSPKGMETLRHFLFDVCGVSADWKMEDLMEEEIKVINKTVASDEHVICALSGGVDS 240

Query: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKG 300
           TVAATLVHKAIG+RLHC+FVDNGLLRYKE+ERVM+TFERDLHLPVTCVDA + FLS+LKG
Sbjct: 241 TVAATLVHKAIGDRLHCIFVDNGLLRYKEQERVMDTFERDLHLPVTCVDASERFLSELKG 300

Query: 301 AVDPEVKRKIIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHS 360
            VDPE KRKIIG+EFI+IFD FA +LE K GKKP++LVQGTLYPDVIESCPPPG+  THS
Sbjct: 301 VVDPETKRKIIGREFINIFDQFAQELEKKHGKKPAFLVQGTLYPDVIESCPPPGTDRTHS 360

Query: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420
           HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVR+LGRILNVP  FLKRHPFPGPGLAVRV
Sbjct: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRELGRILNVPVGFLKRHPFPGPGLAVRV 420

Query: 421 LGDVTEGNALDVLRQVDEIFIQSIKDAGLYDSIWQAFAVFLPIKSVGVQGDQRTHSHVVA 480
           LGDVT+GNAL+VLRQVDEIFIQSI+DAGLYDSIWQAFAVFLP++SVGVQGD+RTHSHVVA
Sbjct: 421 LGDVTQGNALEVLRQVDEIFIQSIRDAGLYDSIWQAFAVFLPVRSVGVQGDKRTHSHVVA 480

Query: 481 LRAVTSQDGMTADWFNFEHQFLDNVSRKICNSVRGVNRVVQDITSKPPSTIEWE 535
           LRAVTSQDGMTADWFNFEH+FLD+VSRKICNSV+GVNRVV DITSKPPSTIEWE
Sbjct: 481 LRAVTSQDGMTADWFNFEHKFLDDVSRKICNSVQGVNRVVLDITSKPPSTIEWE 534

BLAST of Spg029358 vs. TAIR 10
Match: AT1G63660.2 (GMP synthase (glutamine-hydrolyzing), putative / glutamine amidotransferase, putative )

HSP 1 Score: 715.3 bits (1845), Expect = 3.6e-206
Identity = 342/434 (78.80%), Postives = 391/434 (90.09%), Query Frame = 0

Query: 1   MDPKELKSDLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKAITDLNPHVVILSGG 60
           M+   +K D VLILD+GSQYTHLITRRIRSL+VFSL ISGTSSLK+IT  NP VVILSGG
Sbjct: 1   METPTMKPDTVLILDYGSQYTHLITRRIRSLNVFSLVISGTSSLKSITSYNPRVVILSGG 60

Query: 61  PHSVHASDSPSFASGFVEWAEANGVFVLGICYGLQLIVQRLGGVVKIGEKQEYGRMEIEV 120
           PHSVHA D+PSF  GF+EWAE+NGV VLGICYGLQLIVQ+LGGVV  GE +EYG+MEIEV
Sbjct: 61  PHSVHALDAPSFPEGFIEWAESNGVSVLGICYGLQLIVQKLGGVVVEGESKEYGKMEIEV 120

Query: 121 EKANGLFGTKKVGDRQAVWMSHGDEAAKLPEGFEVVARSQQGAVAAVENHSKKFYGLQYH 180
           +  + +FG++  G++Q VWMSHGDEA KLPEGFEVVA+S QGAVAA+E+  KK YGLQYH
Sbjct: 121 KGKSEIFGSESGGEKQMVWMSHGDEAVKLPEGFEVVAQSAQGAVAALESRKKKIYGLQYH 180

Query: 181 PEVTHSPEGMETLRYFLFDVCGITASWRMENILDEELKIIKETVGPDDHVICALSGGVDS 240
           PEVTHSP+GMETLR+FLFDVCG++A W+ME++++EE+K+I +TV  D+HVICALSGGVDS
Sbjct: 181 PEVTHSPKGMETLRHFLFDVCGVSADWKMEDLMEEEIKVINKTVASDEHVICALSGGVDS 240

Query: 241 TVAATLVHKAIGERLHCVFVDNGLLRYKERERVMETFERDLHLPVTCVDAVDHFLSKLKG 300
           TVAATLVHKAIG+RLHC+FVDNGLLRYKE+ERVM+TFERDLHLPVTCVDA + FLS+LKG
Sbjct: 241 TVAATLVHKAIGDRLHCIFVDNGLLRYKEQERVMDTFERDLHLPVTCVDASERFLSELKG 300

Query: 301 AVDPEVKRKIIGKEFISIFDSFAHDLELKIGKKPSYLVQGTLYPDVIESCPPPGSGSTHS 360
            VDPE KRKIIG+EFI+IFD FA +LE K GKKP++LVQGTLYPDVIESCPPPG+  THS
Sbjct: 301 VVDPETKRKIIGREFINIFDQFAQELEKKHGKKPAFLVQGTLYPDVIESCPPPGTDRTHS 360

Query: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLKRHPFPGPGLAVRV 420
           HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVR+LGRILNVP  FLKRHPFPGPGLAVRV
Sbjct: 361 HTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRELGRILNVPVGFLKRHPFPGPGLAVRV 420

Query: 421 LGDVTEGNALDVLR 435
           LGDVT+GNAL+VLR
Sbjct: 421 LGDVTQGNALEVLR 434

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022958390.19.7e-30296.07uncharacterized protein LOC111459626 [Cucurbita moschata][more]
XP_023522242.11.3e-30195.88uncharacterized protein LOC111786132 isoform X1 [Cucurbita pepo subsp. pepo] >XP... [more]
XP_038875856.12.2e-30195.32GMP synthase [glutamine-hydrolyzing] [Benincasa hispida][more]
XP_022995491.13.7e-30195.69uncharacterized protein LOC111491012 [Cucurbita maxima][more]
XP_022159721.13.7e-30195.13uncharacterized protein LOC111026060 [Momordica charantia][more]
Match NameE-valueIdentityDescription
O666011.6e-15353.90GMP synthase [glutamine-hydrolyzing] OS=Aquifex aeolicus (strain VF5) OX=224324 ... [more]
Q3AD701.5e-14850.95GMP synthase [glutamine-hydrolyzing] OS=Carboxydothermus hydrogenoformans (strai... [more]
C0QYF19.8e-14850.57GMP synthase [glutamine-hydrolyzing] OS=Brachyspira hyodysenteriae (strain ATCC ... [more]
B6JHM41.7e-14752.75GMP synthase [glutamine-hydrolyzing] OS=Oligotropha carboxidovorans (strain ATCC... [more]
Q1QKC31.1e-14651.23GMP synthase [glutamine-hydrolyzing] OS=Nitrobacter hamburgensis (strain DSM 102... [more]
Match NameE-valueIdentityDescription
A0A6J1H3B64.7e-30296.07Glutamine amidotransferase OS=Cucurbita moschata OX=3662 GN=LOC111459626 PE=3 SV... [more]
A0A6J1K2231.8e-30195.69Glutamine amidotransferase OS=Cucurbita maxima OX=3661 GN=LOC111491012 PE=3 SV=1[more]
A0A6J1E4S41.8e-30195.13Glutamine amidotransferase OS=Momordica charantia OX=3673 GN=LOC111026060 PE=3 S... [more]
A0A0A0KKJ02.3e-30195.13Glutamine amidotransferase OS=Cucumis sativus OX=3659 GN=Csa_5G153080 PE=3 SV=1[more]
A0A5A7THI72.6e-30094.94Glutamine amidotransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
Match NameE-valueIdentityDescription
AT1G63660.15.0e-26481.27GMP synthase (glutamine-hydrolyzing), putative / glutamine amidotransferase, put... [more]
AT1G63660.23.6e-20678.80GMP synthase (glutamine-hydrolyzing), putative / glutamine amidotransferase, put... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00096GATASEcoord: 176..189
score: 45.26
coord: 55..64
score: 41.34
coord: 86..97
score: 50.49
NoneNo IPR availableGENE3D3.30.300.10coord: 414..534
e-value: 1.1E-45
score: 157.3
NoneNo IPR availablePANTHERPTHR11922GMP SYNTHASE-RELATEDcoord: 2..534
NoneNo IPR availablePANTHERPTHR11922:SF4GMP SYNTHASE (GLUTAMINE-HYDROLYZING), PUTATIVE / GLUTAMINE AMIDOTRANSFERASE, PUTATIVE-RELATEDcoord: 2..534
NoneNo IPR availablePROSITEPS51273GATASE_TYPE_1coord: 10..206
score: 29.512321
NoneNo IPR availableSUPERFAMILY54810GMP synthetase C-terminal dimerisation domaincoord: 413..534
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 196..428
IPR001674GMP synthase, C-terminalPFAMPF00958GMP_synt_Ccoord: 443..533
e-value: 4.5E-42
score: 142.0
IPR001674GMP synthase, C-terminalTIGRFAMTIGR00884TIGR00884coord: 211..534
e-value: 5.1E-136
score: 450.9
IPR001674GMP synthase, C-terminalCDDcd01997GMP_synthase_Ccoord: 229..533
e-value: 0.0
score: 530.171
IPR017926Glutamine amidotransferasePFAMPF00117GATasecoord: 13..199
e-value: 1.4E-32
score: 113.0
IPR029062Class I glutamine amidotransferase-likeGENE3D3.40.50.880coord: 4..204
e-value: 1.0E-44
score: 154.9
IPR029062Class I glutamine amidotransferase-likeSUPERFAMILY52317Class I glutamine amidotransferase-likecoord: 9..237
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 208..413
e-value: 3.7E-74
score: 250.9
IPR004739GMP synthase, glutamine amidotransferaseTIGRFAMTIGR00888TIGR00888coord: 11..203
e-value: 3.1E-62
score: 207.3
IPR004739GMP synthase, glutamine amidotransferaseCDDcd01742GATase1_GMP_Synthasecoord: 11..197
e-value: 2.588E-86
score: 262.472
IPR022955GMP synthaseHAMAPMF_00344GMP_synthasecoord: 8..534
score: 48.704674
IPR025777GMP synthetase ATP pyrophosphatase domainPROSITEPS51553GMPS_ATP_PPASEcoord: 207..408
score: 80.954155

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg029358.1Spg029358.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006541 glutamine metabolic process
biological_process GO:0006177 GMP biosynthetic process
biological_process GO:0006164 purine nucleotide biosynthetic process
cellular_component GO:0005829 cytosol
molecular_function GO:0005524 ATP binding
molecular_function GO:0003921 GMP synthase activity
molecular_function GO:0016462 pyrophosphatase activity
molecular_function GO:0003922 GMP synthase (glutamine-hydrolyzing) activity