Spg029261 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg029261
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAquaporin NIP3-1
Locationscaffold12: 32957403 .. 32960962 (+)
RNA-Seq ExpressionSpg029261
SyntenySpg029261
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCAACTATGATTTGAGTCATTTGATGAATTTAAGGATCAAAGAATATTTTGCAACATACTGGTATTCTCTTAATCTTCTTCAATCCAAGGCCTTTCTCTTTAGCATTATTTTTGCATGGGCTTGACAATTTCACAAGTATGAAGTAAGTTAGTTGATTATTCTCAACCAAAAGTTTGCAACAACAATCTCTTAGATTATCAATTATGTAAGAATATAACATTGGAAAGTAGTACATAGGATAGTTTGATATCAAACTACTGAAAATATATAATGCTTAAATTTTTTATTTACTTTAATTTATTTTATGATTTTAACAATATCTGCAGGATGGATTCAAATGTCTAATATTTTGATCGAGAGTATATATCTTAATTAATTGAGCTATACTAATTAGGTTGACTTATTTTTATTTTGTTTTTTATCCAAGTGATGTGAAGCTTCTTTCTATTTGGGTTTCTATTTAGGGTGCATATAACAAAATGGAAATTTGAAAGAAAAATACTTTGATAGAAATCAAAGTTTGCATTTTGTGTTTGATATCAAATTTAAAATCTAAAAAACACTTTATAAATACAATCACCAAGTAGAAATTCACTGAATCTAGATAAAATACCAAATAACTCTTATGTATATGAGTCTTCCAAGTATAAAATAGAGGTTTCTTACCTTGGCTTCAAAAAACGGTTTCTTTTCTTCTTATCTTTTGTTTATCAGTAAAACCTAGAGCTGGTTTATCAAGTAAAGCATAATCCCGTAAAAAAAAAAGGAATGTCAGAGTCAGAAAGTGGGATATTGGTTTCATCGAGGGCGGAGTCCCAATGTTCAAGCCTCCGAGTGGATTCATTGTCGTACGATCAAAAACAAATGTCCCAATGCGAGTGCTTGCCTGTTAATGCCCCAAAGAGGAGCCAGGGTCACATGTGCTTCACTCACTTCCCTACTCTTGGTGTCTCTCTCACTAGAAAGGTACCTCTTTTTGTATATAGCTCTTTTGTTTCCTCATGTTCAAACATGTAAAGGATAAATATTTTAACTGTGGATCTCAGCTCAAGGACCAATCCATTTGGGTAACATAAATATTTAGATGGGACCGTTGGGCTGGCCCAAAAGACATTTGATTGGGCTTTTAGCAACAAATTTTTATATTTTAATTGATAACAATTTTAATTGATAACAAAATTAGAAGTGGAATACAACAAATACATGTTTTTTTTCTTGAAAGACTAATACATAGTTTTTAAAATGAAAAGAAAATTAAAGGTTTTTTAAAGGAAAAAGTAACCAAAAAAAATCACAAATGCAAAATGGATTTGAGTTCAAACAATTAATCCAAGTAGGTTCCAATACAATTGTTTTAGTTTAACAATATGGGATTGAAGAATTTGATTGTAAGCACACACTTATACTTAGTTAACCTTTGTTTTTATGGATGGTTTTAATATAACTTGGTTTTAAAGTAAGTCTATATATATTTTATTTTTGGAAAGTAAGTATAGGGCTCAATTAAATGTTGCTTCGATGAGCTATATGTGTAGACAATGTTGAATTTTAATTTATGCCATTAAAAATTTGTAGTTACAAACTTGCAAATATAGAAATTATGAGTCAAAGTGAGTTGAGTATGACATGTATTCTCGATTGTGCAGTCAAAGGCTTTAACTCCTATCCTAAATATTGTTGAAATAAAAAATAATATATATGTGTTGATTACATGTGATTGATTTAATTTTTCCTAAATGAGAAGTTCTTTTTAACATTAGAGCATGGAAAAGTTTAGAATTCACATTCGAGGGTATGTGTCAATTATCGTTGACCTATGCTCGTAAAATTTAATTTTATTTCATATCTATTAAAATTTAAAACTATAAAGTAGGTGTAAAAGTAAAACATAATTCCCTGTTGACTATTTGACTGATGTCCAGCTTGGAGCAGAATTCGTGGGCACATTCATCCTCATATTTGCCGCAACAGCCGCACCCATCATCAACCAAAAGTACAACGCCGCCCTGTCCCTGATCGGAAACGCCGCATGCTCCGGCCTAGCGGTGATGATCGTAATTATGTCAACCGGTCACATCTCCGGCGCCCACCTGAACCCGTCCCTCACCATCGCCTTCGCCGCCCTCCGCCACTTCCCATGGGTCCAAGTGCCGGCCTACATCGCCGCCCAAGTCTCCGCCTCCATCTGCGCCTCCTACGCTCTCAAAGGAGTATTTCACCCTTTCATGTCCGGCGGCGTCACCGTCCCCTCCGTCGGCACCGGCCAGGCTTTTGCTCTCGAATTCCTCATCACTTTCAATCTCTTGTTTGTCGTCACTGCTGTCGCTACTGATACTCGCGCTGTAAGCAAAAAGTCTCTCTGTTCTTTACAATGACTCTCAACATTTAATTATGGTTTTTTTTTTTCCTTTTTGCAGGTTGGAGAGTTAGCCGGAATTGCAGTCGGAGCTACAGTGCTGCTCAACATTCTTATCGCAGGGTCAATATTTCTAATTTTCATCTTCTTTAAATTTTTGTCTTAATTTTTATCTCTTCCAAAAAGTAATACACTCAAACTATTCAATTTTTTAAATACAATCTTTTCAATTGTTAAAACGGTTTTAAGAACAAATTTGAAAGTAAGTTTGAAAAAAAATTAAACTTTTATATATATATATATTAGCCTGGTCTTCTCTTTTTTCCTTCACTATGGTTACTTATCAATTTAAGCTTTTGAGTTGAATAGTGTTTTAATATTGTATCCATTTAAGTTTTACCTTCAATAGTAGTAGTATTATCTACTCTTAGGAAATTAGAGGAGGATATATCCTATATCTAAGTGAGGCCAACTACCTCATTAGTATATAATAGGACCTAATTTTAGATAAAATCTAAGAAAACAAAATCATGAAATCTTATGTCCAAATTGGACAATATCATACCAATATATAAATATAGAGAGGTTTTCTAAAATTAACGTAAGCAGAAAATAAACTCTATCATATCATTGTAGAAATATGGAGAGGTTTGTTGTCCTAACTCAACATATCAGATTAAGTTTATTTTTGGTTTAGGGATGATTTGTGATATCGTATAACTTGATTTCGTTTTTCTCTTTACGAATAATTGATACTTTTCGACGGCATAACAGGCCATCCACCGGTGGTTCGATGAATCCAGTGCGGACTCTGGGCCCCGCCGTGGCCGCCGGAAATTACAGAGAATTGTGGGTGTACATGGTGGCTCCTACACTTGGGGCAATTGTCGGCGCCGGAACCTACGCCGCCGTTAAGCTCAAAGATGATAAACTGGAGGTTCCGTCGCCGGTGAGGAGCTTCCGTCGATGAGAACGGGTGGTAGCTATGTTTTGTTTAAATCTGCTGCTTGTATTTTCTTTCATCTTACTTAGAGTTATTTAATGTAGATTTTTTCAAACAATTAGGATTTTTGAAATAGAAGAAATAGGTATGTTATTGGTTTTTTTTTTTTTTTTTTTGAGTTTACGTAGGTTATTAGTTGCTTAAATTAGTATTTTCTTTTACTCGTGATTCAATATTATAGATGTATATAACTGTTTC

mRNA sequence

ATGTCAGAGTCAGAAAGTGGGATATTGGTTTCATCGAGGGCGGAGTCCCAATGTTCAAGCCTCCGAGTGGATTCATTGTCGTACGATCAAAAACAAATGTCCCAATGCGAGTGCTTGCCTGTTAATGCCCCAAAGAGGAGCCAGGGTCACATGTGCTTCACTCACTTCCCTACTCTTGGTGTCTCTCTCACTAGAAAGCTTGGAGCAGAATTCGTGGGCACATTCATCCTCATATTTGCCGCAACAGCCGCACCCATCATCAACCAAAAGTACAACGCCGCCCTGTCCCTGATCGGAAACGCCGCATGCTCCGGCCTAGCGGTGATGATCGTAATTATGTCAACCGGTCACATCTCCGGCGCCCACCTGAACCCGTCCCTCACCATCGCCTTCGCCGCCCTCCGCCACTTCCCATGGGTCCAAGTGCCGGCCTACATCGCCGCCCAAGTCTCCGCCTCCATCTGCGCCTCCTACGCTCTCAAAGGAGTATTTCACCCTTTCATGTCCGGCGGCGTCACCGTCCCCTCCGTCGGCACCGGCCAGGCTTTTGCTCTCGAATTCCTCATCACTTTCAATCTCTTGTTTGTCGTCACTGCTGTCGCTACTGATACTCGCGCTGTTGGAGAGTTAGCCGGAATTGCAGTCGGAGCTACAGTGCTGCTCAACATTCTTATCGCAGGGCCATCCACCGGTGGTTCGATGAATCCAGTGCGGACTCTGGGCCCCGCCGTGGCCGCCGGAAATTACAGAGAATTGTGGGTGTACATGGTGGCTCCTACACTTGGGGCAATTGTCGGCGCCGGAACCTACGCCGCCGTTAAGCTCAAAGATGATAAACTGGAGGTTCCGTCGCCGGTGAGGAGCTTCCGTCGATGA

Coding sequence (CDS)

ATGTCAGAGTCAGAAAGTGGGATATTGGTTTCATCGAGGGCGGAGTCCCAATGTTCAAGCCTCCGAGTGGATTCATTGTCGTACGATCAAAAACAAATGTCCCAATGCGAGTGCTTGCCTGTTAATGCCCCAAAGAGGAGCCAGGGTCACATGTGCTTCACTCACTTCCCTACTCTTGGTGTCTCTCTCACTAGAAAGCTTGGAGCAGAATTCGTGGGCACATTCATCCTCATATTTGCCGCAACAGCCGCACCCATCATCAACCAAAAGTACAACGCCGCCCTGTCCCTGATCGGAAACGCCGCATGCTCCGGCCTAGCGGTGATGATCGTAATTATGTCAACCGGTCACATCTCCGGCGCCCACCTGAACCCGTCCCTCACCATCGCCTTCGCCGCCCTCCGCCACTTCCCATGGGTCCAAGTGCCGGCCTACATCGCCGCCCAAGTCTCCGCCTCCATCTGCGCCTCCTACGCTCTCAAAGGAGTATTTCACCCTTTCATGTCCGGCGGCGTCACCGTCCCCTCCGTCGGCACCGGCCAGGCTTTTGCTCTCGAATTCCTCATCACTTTCAATCTCTTGTTTGTCGTCACTGCTGTCGCTACTGATACTCGCGCTGTTGGAGAGTTAGCCGGAATTGCAGTCGGAGCTACAGTGCTGCTCAACATTCTTATCGCAGGGCCATCCACCGGTGGTTCGATGAATCCAGTGCGGACTCTGGGCCCCGCCGTGGCCGCCGGAAATTACAGAGAATTGTGGGTGTACATGGTGGCTCCTACACTTGGGGCAATTGTCGGCGCCGGAACCTACGCCGCCGTTAAGCTCAAAGATGATAAACTGGAGGTTCCGTCGCCGGTGAGGAGCTTCCGTCGATGA

Protein sequence

MSESESGILVSSRAESQCSSLRVDSLSYDQKQMSQCECLPVNAPKRSQGHMCFTHFPTLGVSLTRKLGAEFVGTFILIFAATAAPIINQKYNAALSLIGNAACSGLAVMIVIMSTGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASYALKGVFHPFMSGGVTVPSVGTGQAFALEFLITFNLLFVVTAVATDTRAVGELAGIAVGATVLLNILIAGPSTGGSMNPVRTLGPAVAAGNYRELWVYMVAPTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR
Homology
BLAST of Spg029261 vs. NCBI nr
Match: XP_008437497.1 (PREDICTED: probable aquaporin NIP5-1 [Cucumis melo])

HSP 1 Score: 460.3 bits (1183), Expect = 1.2e-125
Identity = 243/298 (81.54%), Postives = 262/298 (87.92%), Query Frame = 0

Query: 1   MSESESGILVSSRAESQ-------CSSLRVDSLSYDQKQMSQCECLPVNAPKRSQGHMCF 60
           M ESESG  V+S   +         S LRVDSLSYD+K M +C+CLPVNAP   Q H CF
Sbjct: 1   MPESESGTPVASAPATPGTPGGPLFSGLRVDSLSYDRKSMPRCKCLPVNAPTWGQPHTCF 60

Query: 61  THFPTLGVSLTRKLGAEFVGTFILIFAATAAPIINQKYNAALSLIGNAACSGLAVMIVIM 120
           T FP   VSLTRKLGAEFVGTFILIFAATA PI+NQKYN   +LIGNAAC+GLAVMIVI+
Sbjct: 61  TDFPAPDVSLTRKLGAEFVGTFILIFAATAGPIVNQKYNGVETLIGNAACAGLAVMIVIL 120

Query: 121 STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASYALKGVFHPFMSGGVT 180
           STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICAS+ALKGVFHPFMSGGVT
Sbjct: 121 STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASFALKGVFHPFMSGGVT 180

Query: 181 VPSVGTGQAFALEFLITFNLLFVVTAVATDTRAVGELAGIAVGATVLLNILIAGPSTGGS 240
           VPSV TGQAFALEF+ITFNLLFVVTAVATDTRAVGELAGIAVGATV+LNIL+AGPS+GGS
Sbjct: 181 VPSVSTGQAFALEFIITFNLLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSSGGS 240

Query: 241 MNPVRTLGPAVAAGNYRELWVYMVAPTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR 292
           MNPVRTLGPAVAAGNYR LWVY+VAPTLGAI+GAGTY AVKL+DD++E PS VRSFRR
Sbjct: 241 MNPVRTLGPAVAAGNYRALWVYLVAPTLGAIIGAGTYTAVKLRDDEVEAPSQVRSFRR 298

BLAST of Spg029261 vs. NCBI nr
Match: XP_038875525.1 (probable aquaporin NIP5-1 [Benincasa hispida])

HSP 1 Score: 459.1 bits (1180), Expect = 2.8e-125
Identity = 243/298 (81.54%), Postives = 262/298 (87.92%), Query Frame = 0

Query: 1   MSESESGILVSSRAESQ-------CSSLRVDSLSYDQKQMSQCECLPVNAPKRSQGHMCF 60
           M ESESG  V+S   +         S LRVDSLSYD+K M +C+CLPVNAP   Q H CF
Sbjct: 1   MPESESGTPVASAPATPGTPGGPLFSGLRVDSLSYDRKSMPRCKCLPVNAPTWGQPHTCF 60

Query: 61  THFPTLGVSLTRKLGAEFVGTFILIFAATAAPIINQKYNAALSLIGNAACSGLAVMIVIM 120
           T FP   VSLTRKLGAEFVGTFILIFAATA PI+NQKYN   +LIGNAAC+GLAVMIVI+
Sbjct: 61  TDFPAPDVSLTRKLGAEFVGTFILIFAATAGPIVNQKYNGVETLIGNAACAGLAVMIVIL 120

Query: 121 STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASYALKGVFHPFMSGGVT 180
           STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICAS+ALKGVFHPFMSGGVT
Sbjct: 121 STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASFALKGVFHPFMSGGVT 180

Query: 181 VPSVGTGQAFALEFLITFNLLFVVTAVATDTRAVGELAGIAVGATVLLNILIAGPSTGGS 240
           VPSV TGQAFALEF+ITFNLLFVVTAVATDTRAVGELAGIAVGATV+LNIL+AGPS+GGS
Sbjct: 181 VPSVSTGQAFALEFIITFNLLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSSGGS 240

Query: 241 MNPVRTLGPAVAAGNYRELWVYMVAPTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR 292
           MNPVRTLGPAVAAGNYR LWVY+VAPTLGAIVGAGTY AVKL++D++E PS VRSFRR
Sbjct: 241 MNPVRTLGPAVAAGNYRALWVYLVAPTLGAIVGAGTYTAVKLREDEVEAPSQVRSFRR 298

BLAST of Spg029261 vs. NCBI nr
Match: XP_022957899.1 (probable aquaporin NIP5-1 [Cucurbita moschata] >XP_023534473.1 probable aquaporin NIP5-1 [Cucurbita pepo subsp. pepo] >KAG6605632.1 putative aquaporin NIP5-1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035542.1 putative aquaporin NIP5-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 458.4 bits (1178), Expect = 4.7e-125
Identity = 243/298 (81.54%), Postives = 261/298 (87.58%), Query Frame = 0

Query: 1   MSESESGILVSSRAESQ-------CSSLRVDSLSYDQKQMSQCECLPVNAPKRSQGHMCF 60
           M ESESG  V S   +         S LRVDSLSYD+K M +C+CLPVNAP   Q H CF
Sbjct: 1   MPESESGTPVPSAPATPGTPGGPLFSGLRVDSLSYDRKSMPRCKCLPVNAPTWGQPHTCF 60

Query: 61  THFPTLGVSLTRKLGAEFVGTFILIFAATAAPIINQKYNAALSLIGNAACSGLAVMIVIM 120
           T FP   VSLTRKLGAEFVGTFILIFAATA PI+NQKYN A +LIGNAAC+GLAVMIVI+
Sbjct: 61  TDFPVPDVSLTRKLGAEFVGTFILIFAATAGPIVNQKYNGAETLIGNAACAGLAVMIVIL 120

Query: 121 STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASYALKGVFHPFMSGGVT 180
           STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICAS+ALKGVFHPFMSGGVT
Sbjct: 121 STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASFALKGVFHPFMSGGVT 180

Query: 181 VPSVGTGQAFALEFLITFNLLFVVTAVATDTRAVGELAGIAVGATVLLNILIAGPSTGGS 240
           VPSV TGQAFALEF ITFNLLFVVTAVATDTRAVGELAGIAVGATV++NIL+AGPS+GGS
Sbjct: 181 VPSVSTGQAFALEFFITFNLLFVVTAVATDTRAVGELAGIAVGATVMVNILVAGPSSGGS 240

Query: 241 MNPVRTLGPAVAAGNYRELWVYMVAPTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR 292
           MNPVRTLGPAVAAGNYR LWVY+VAPTLGAIVGAGTY AVKL+DD+++ PS VRSFRR
Sbjct: 241 MNPVRTLGPAVAAGNYRALWVYLVAPTLGAIVGAGTYTAVKLRDDEVDAPSQVRSFRR 298

BLAST of Spg029261 vs. NCBI nr
Match: XP_022996063.1 (probable aquaporin NIP5-1 [Cucurbita maxima])

HSP 1 Score: 457.2 bits (1175), Expect = 1.0e-124
Identity = 241/298 (80.87%), Postives = 261/298 (87.58%), Query Frame = 0

Query: 1   MSESESGILVSSRAESQ-------CSSLRVDSLSYDQKQMSQCECLPVNAPKRSQGHMCF 60
           M ESESG  V S   +         S LRVDSLSYD+K M +C+CLPVNAP   Q H CF
Sbjct: 1   MPESESGTPVPSAPATPGTPGGPLFSGLRVDSLSYDRKSMPRCKCLPVNAPTWGQPHTCF 60

Query: 61  THFPTLGVSLTRKLGAEFVGTFILIFAATAAPIINQKYNAALSLIGNAACSGLAVMIVIM 120
           T FP   VSLTRKLGAEFVGTFILIFAATA PI+NQKYN A +LIGNAAC+G+AVMIVI+
Sbjct: 61  TDFPVPDVSLTRKLGAEFVGTFILIFAATAGPIVNQKYNGAETLIGNAACAGMAVMIVIL 120

Query: 121 STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASYALKGVFHPFMSGGVT 180
           STGHISGAHLNPSLTIAFAALRHFPW+QVPAYIAAQVSASICAS+ALKGVFHPFMSGGVT
Sbjct: 121 STGHISGAHLNPSLTIAFAALRHFPWIQVPAYIAAQVSASICASFALKGVFHPFMSGGVT 180

Query: 181 VPSVGTGQAFALEFLITFNLLFVVTAVATDTRAVGELAGIAVGATVLLNILIAGPSTGGS 240
           VPSV TGQAFALEF ITFNLLFVVTAVATDTRAVGELAGIAVGATV++NIL+AGPS+GGS
Sbjct: 181 VPSVSTGQAFALEFFITFNLLFVVTAVATDTRAVGELAGIAVGATVMVNILVAGPSSGGS 240

Query: 241 MNPVRTLGPAVAAGNYRELWVYMVAPTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR 292
           MNPVRTLGPAVAAGNYR LWVY+VAPTLGAIVGAGTY AVKL+DD+++ PS VRSFRR
Sbjct: 241 MNPVRTLGPAVAAGNYRALWVYLVAPTLGAIVGAGTYTAVKLRDDEVDAPSQVRSFRR 298

BLAST of Spg029261 vs. NCBI nr
Match: XP_004145889.1 (probable aquaporin NIP5-1 [Cucumis sativus] >KGN49946.1 hypothetical protein Csa_000397 [Cucumis sativus])

HSP 1 Score: 455.7 bits (1171), Expect = 3.0e-124
Identity = 240/298 (80.54%), Postives = 261/298 (87.58%), Query Frame = 0

Query: 1   MSESESGILVSSRAESQ-------CSSLRVDSLSYDQKQMSQCECLPVNAPKRSQGHMCF 60
           M ESESG  V+S   +         S LRVDSLSYD+K M +C+CLPVNAP   Q H CF
Sbjct: 1   MPESESGTPVASAPATPGTPGGPLFSGLRVDSLSYDRKSMPRCKCLPVNAPTWGQPHTCF 60

Query: 61  THFPTLGVSLTRKLGAEFVGTFILIFAATAAPIINQKYNAALSLIGNAACSGLAVMIVIM 120
           T FP   VSLTRKLGAEFVGTFILIFAATA PI+NQKYN   +LIGNAAC+GLAVMIVI+
Sbjct: 61  TDFPAPDVSLTRKLGAEFVGTFILIFAATAGPIVNQKYNGVETLIGNAACAGLAVMIVIL 120

Query: 121 STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASYALKGVFHPFMSGGVT 180
           STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICAS+ALKGVFHPFMSGGVT
Sbjct: 121 STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASFALKGVFHPFMSGGVT 180

Query: 181 VPSVGTGQAFALEFLITFNLLFVVTAVATDTRAVGELAGIAVGATVLLNILIAGPSTGGS 240
           VPSV  GQAFALEF+ITFNLLFVVTAVATDTRAVGELAGIAVGATV+LNIL+AGPS+GGS
Sbjct: 181 VPSVSIGQAFALEFIITFNLLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSSGGS 240

Query: 241 MNPVRTLGPAVAAGNYRELWVYMVAPTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR 292
           MNPVRTLGPAVAAGNY+ LWVY+VAPTLGAI+GAGTY AVKL+DD+++ PS VRSFRR
Sbjct: 241 MNPVRTLGPAVAAGNYKALWVYLVAPTLGAIIGAGTYTAVKLRDDEVDAPSQVRSFRR 298

BLAST of Spg029261 vs. ExPASy Swiss-Prot
Match: Q9SV84 (Probable aquaporin NIP5-1 OS=Arabidopsis thaliana OX=3702 GN=NIP5-1 PE=2 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 5.1e-114
Identity = 214/274 (78.10%), Postives = 236/274 (86.13%), Query Frame = 0

Query: 19  SSLRVDSLSYD-QKQMSQCECLPVNAPKRSQGHMCFTHFPTLGVSLTRKLGAEFVGTFIL 78
           + +RVDS+S+D +K   +C+CLPV      Q   CFT FP+  VSLTRKLGAEFVGTFIL
Sbjct: 31  TGMRVDSMSFDHRKPTPRCKCLPVMGSTWGQHDTCFTDFPSPDVSLTRKLGAEFVGTFIL 90

Query: 79  IFAATAAPIINQKYNAALSLIGNAACSGLAVMIVIMSTGHISGAHLNPSLTIAFAALRHF 138
           IF ATA PI+NQKY+ A +LIGNAAC+GLAVMI+I+STGHISGAHLNPSLTIAFAALRHF
Sbjct: 91  IFTATAGPIVNQKYDGAETLIGNAACAGLAVMIIILSTGHISGAHLNPSLTIAFAALRHF 150

Query: 139 PWVQVPAYIAAQVSASICASYALKGVFHPFMSGGVTVPSVGTGQAFALEFLITFNLLFVV 198
           PW  VPAYIAAQVSASICAS+ALKGVFHPFMSGGVT+PSV  GQAFALEF+ITF LLFVV
Sbjct: 151 PWAHVPAYIAAQVSASICASFALKGVFHPFMSGGVTIPSVSLGQAFALEFIITFILLFVV 210

Query: 199 TAVATDTRAVGELAGIAVGATVLLNILIAGPSTGGSMNPVRTLGPAVAAGNYRELWVYMV 258
           TAVATDTRAVGELAGIAVGATV+LNIL+AGPSTGGSMNPVRTLGPAVA+GNYR LWVY+V
Sbjct: 211 TAVATDTRAVGELAGIAVGATVMLNILVAGPSTGGSMNPVRTLGPAVASGNYRSLWVYLV 270

Query: 259 APTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR 292
           APTLGAI GA  Y  VKL D   + P PVRSFRR
Sbjct: 271 APTLGAISGAAVYTGVKLNDSVTDPPRPVRSFRR 304

BLAST of Spg029261 vs. ExPASy Swiss-Prot
Match: Q0IWF3 (Aquaporin NIP3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-1 PE=2 SV=2)

HSP 1 Score: 392.9 bits (1008), Expect = 3.2e-108
Identity = 202/274 (73.72%), Postives = 227/274 (82.85%), Query Frame = 0

Query: 22  RVDSLSYDQKQMSQCECLPVNAPK--RSQGHMCFTHFPTLGVSLTRKLGAEFVGTFILIF 81
           RVDSLSY++K M +C+CLP    +      H C    P   VSLTRKLGAEFVGTFILIF
Sbjct: 38  RVDSLSYERKSMPRCKCLPAAVAEAWAPSAHGCVVEIPAPDVSLTRKLGAEFVGTFILIF 97

Query: 82  AATAAPIINQKYNAALSLIGNAACSGLAVMIVIMSTGHISGAHLNPSLTIAFAALRHFPW 141
            ATAAPI+NQKY  A+S  GNAAC+GLAV  +I+STGHISGAHLNPSLTIAFAALRHFPW
Sbjct: 98  FATAAPIVNQKYGGAISPFGNAACAGLAVTTIILSTGHISGAHLNPSLTIAFAALRHFPW 157

Query: 142 VQVPAYIAAQVSASICASYALKGVFHPFMSGGVTV--PSVGTGQAFALEFLITFNLLFVV 201
           +QVPAY+A QV  SICA +ALKGVFHPF+SGGVTV  P++ T QAF  EF+ITFNLLFVV
Sbjct: 158 LQVPAYVAVQVLGSICAGFALKGVFHPFLSGGVTVPDPTISTAQAFFTEFIITFNLLFVV 217

Query: 202 TAVATDTRAVGELAGIAVGATVLLNILIAGPSTGGSMNPVRTLGPAVAAGNYRELWVYMV 261
           TAVATDTRAVGELAGIAVGA V LNILIAGP+TGGSMNPVRTLGPAVAAGNYR+LW+Y++
Sbjct: 218 TAVATDTRAVGELAGIAVGAAVTLNILIAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLI 277

Query: 262 APTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR 292
           APTLGA+ GAG Y AVKL+D+  E P P RSFRR
Sbjct: 278 APTLGAVAGAGVYTAVKLRDENGETPRPQRSFRR 311

BLAST of Spg029261 vs. ExPASy Swiss-Prot
Match: Q9ATN1 (Aquaporin NIP3-1 OS=Zea mays OX=4577 GN=NIP3-1 PE=2 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 6.6e-106
Identity = 200/274 (72.99%), Postives = 228/274 (83.21%), Query Frame = 0

Query: 22  RVDSLSYDQKQMSQCECLPVNAPK--RSQGHMCFTHFPTLGVSLTRKLGAEFVGTFILIF 81
           RVDSLSY++K M +C+CLP+ A +      H C    P   VSLTRKLGAEFVGTFILIF
Sbjct: 29  RVDSLSYERKSMPRCKCLPLPAVEGWGVATHTCVVEIPAPDVSLTRKLGAEFVGTFILIF 88

Query: 82  AATAAPIINQKYNAALSLIGNAACSGLAVMIVIMSTGHISGAHLNPSLTIAFAALRHFPW 141
            ATAAPI+NQKY  A+S  GNAAC+GLAV  VI+STGHISGAHLNPSLTIAFAALRHFPW
Sbjct: 89  FATAAPIVNQKYGGAISPFGNAACAGLAVATVILSTGHISGAHLNPSLTIAFAALRHFPW 148

Query: 142 VQVPAYIAAQVSASICASYALKGVFHPFMSGGVTVP--SVGTGQAFALEFLITFNLLFVV 201
           +QVPAY+A Q  AS+CA++ALKGVFHPF+SGGVTVP  +V T QAF  EF+I+FNLLFVV
Sbjct: 149 LQVPAYVAVQALASVCAAFALKGVFHPFLSGGVTVPDATVSTAQAFFTEFIISFNLLFVV 208

Query: 202 TAVATDTRAVGELAGIAVGATVLLNILIAGPSTGGSMNPVRTLGPAVAAGNYRELWVYMV 261
           TAVATDTRAVGELAGIAVGA V LNIL+AGP+TGGSMNPVRTLGPAVAAGNYR+LW+Y++
Sbjct: 209 TAVATDTRAVGELAGIAVGAAVTLNILVAGPTTGGSMNPVRTLGPAVAAGNYRQLWIYLL 268

Query: 262 APTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR 292
           APTLGA+ GA  Y AVKL+D+  E P   RSFRR
Sbjct: 269 APTLGALAGASVYKAVKLRDENGETPRTQRSFRR 302

BLAST of Spg029261 vs. ExPASy Swiss-Prot
Match: Q9SAI4 (Aquaporin NIP6-1 OS=Arabidopsis thaliana OX=3702 GN=NIP6-1 PE=1 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 8.1e-88
Identity = 172/235 (73.19%), Postives = 196/235 (83.40%), Query Frame = 0

Query: 57  PTLGVSLTRKLGAEFVGTFILIFAATAAPIINQKYNAALSLIGNAACSGLAVMIVIMSTG 116
           P   VSL RKLGAEFVGT ILIFA TA  I+NQK + A +LIG AA +GLAVMIVI+STG
Sbjct: 72  PPPNVSLYRKLGAEFVGTLILIFAGTATAIVNQKTDGAETLIGCAASAGLAVMIVILSTG 131

Query: 117 HISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASYALKGVFHPFMSGGVTVPS 176
           HISGAHLNP++TIAFAAL+HFPW  VP YI AQV AS+ A++ALK VF P MSGGVTVP+
Sbjct: 132 HISGAHLNPAVTIAFAALKHFPWKHVPVYIGAQVMASVSAAFALKAVFEPTMSGGVTVPT 191

Query: 177 VGTGQAFALEFLITFNLLFVVTAVATDTRAVGELAGIAVGATVLLNILIAGPSTGGSMNP 236
           VG  QAFALEF+I+FNL+FVVTAVATDTRAVGELAGIAVGATV+LNILIAGP+T  SMNP
Sbjct: 192 VGLSQAFALEFIISFNLMFVVTAVATDTRAVGELAGIAVGATVMLNILIAGPATSASMNP 251

Query: 237 VRTLGPAVAAGNYRELWVYMVAPTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR 292
           VRTLGPA+AA NYR +WVY+ AP LGA++GAGTY  VKL ++  E P   RSFRR
Sbjct: 252 VRTLGPAIAANNYRAIWVYLTAPILGALIGAGTYTIVKLPEED-EAPKERRSFRR 305

BLAST of Spg029261 vs. ExPASy Swiss-Prot
Match: Q84S07 (Aquaporin NIP3-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-3 PE=2 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.8e-58
Identity = 120/251 (47.81%), Postives = 169/251 (67.33%), Query Frame = 0

Query: 29  DQKQMSQCECLPV----NAPKRSQGHMCFTHFPTLGVSLTRKLGAEFVGTFILIFAATAA 88
           D    SQC   P        ++S  H+         V L +K+ AEF GTFILIF   + 
Sbjct: 28  DSNVSSQCHADPAELSDETQQQSLWHLGLRKIIPSSVPLLKKVSAEFFGTFILIFTVLST 87

Query: 89  PIINQKYNAALSLIGNAACSGLAVMIVIMSTGHISGAHLNPSLTIAFAALRHFPWVQVPA 148
            I+++++ +  +L+G A  +GLAV ++++S  HISG HLNP+++IA A   H P   +  
Sbjct: 88  IIMDEQHKSIETLLGIATSAGLAVTVLVLSLIHISGCHLNPAISIAMAVFGHLPSAHLLP 147

Query: 149 YIAAQVSASICASYALKGVFHPFMSGGVTVPSVGTGQAFALEFLITFNLLFVVTAVATDT 208
           YI++Q+  ++ AS+A+KG++HP   G VTVP+VGT +AF +EF+ITF LLF++TA+ATD 
Sbjct: 148 YISSQILGAVAASFAVKGLYHPVNPGIVTVPNVGTVEAFFVEFIITFFLLFIITALATDP 207

Query: 209 RAVGELAGIAVGATVLLNILIAGPSTGGSMNPVRTLGPAVAAGNYRELWVYMVAPTLGAI 268
            AV EL  +AVGATV++NIL+AGPSTG SMNP RT+G A+A G Y ++WVY+VA  LGAI
Sbjct: 208 NAVKELIAVAVGATVMMNILVAGPSTGASMNPARTIGAAIATGRYTQIWVYLVATPLGAI 267

Query: 269 VGAGTYAAVKL 276
            G G Y A+KL
Sbjct: 268 AGTGAYVAIKL 278

BLAST of Spg029261 vs. ExPASy TrEMBL
Match: A0A1S3AU64 (probable aquaporin NIP5-1 OS=Cucumis melo OX=3656 GN=LOC103482897 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 6.0e-126
Identity = 243/298 (81.54%), Postives = 262/298 (87.92%), Query Frame = 0

Query: 1   MSESESGILVSSRAESQ-------CSSLRVDSLSYDQKQMSQCECLPVNAPKRSQGHMCF 60
           M ESESG  V+S   +         S LRVDSLSYD+K M +C+CLPVNAP   Q H CF
Sbjct: 1   MPESESGTPVASAPATPGTPGGPLFSGLRVDSLSYDRKSMPRCKCLPVNAPTWGQPHTCF 60

Query: 61  THFPTLGVSLTRKLGAEFVGTFILIFAATAAPIINQKYNAALSLIGNAACSGLAVMIVIM 120
           T FP   VSLTRKLGAEFVGTFILIFAATA PI+NQKYN   +LIGNAAC+GLAVMIVI+
Sbjct: 61  TDFPAPDVSLTRKLGAEFVGTFILIFAATAGPIVNQKYNGVETLIGNAACAGLAVMIVIL 120

Query: 121 STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASYALKGVFHPFMSGGVT 180
           STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICAS+ALKGVFHPFMSGGVT
Sbjct: 121 STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASFALKGVFHPFMSGGVT 180

Query: 181 VPSVGTGQAFALEFLITFNLLFVVTAVATDTRAVGELAGIAVGATVLLNILIAGPSTGGS 240
           VPSV TGQAFALEF+ITFNLLFVVTAVATDTRAVGELAGIAVGATV+LNIL+AGPS+GGS
Sbjct: 181 VPSVSTGQAFALEFIITFNLLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSSGGS 240

Query: 241 MNPVRTLGPAVAAGNYRELWVYMVAPTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR 292
           MNPVRTLGPAVAAGNYR LWVY+VAPTLGAI+GAGTY AVKL+DD++E PS VRSFRR
Sbjct: 241 MNPVRTLGPAVAAGNYRALWVYLVAPTLGAIIGAGTYTAVKLRDDEVEAPSQVRSFRR 298

BLAST of Spg029261 vs. ExPASy TrEMBL
Match: A0A6J1H3D6 (probable aquaporin NIP5-1 OS=Cucurbita moschata OX=3662 GN=LOC111459297 PE=3 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 2.3e-125
Identity = 243/298 (81.54%), Postives = 261/298 (87.58%), Query Frame = 0

Query: 1   MSESESGILVSSRAESQ-------CSSLRVDSLSYDQKQMSQCECLPVNAPKRSQGHMCF 60
           M ESESG  V S   +         S LRVDSLSYD+K M +C+CLPVNAP   Q H CF
Sbjct: 1   MPESESGTPVPSAPATPGTPGGPLFSGLRVDSLSYDRKSMPRCKCLPVNAPTWGQPHTCF 60

Query: 61  THFPTLGVSLTRKLGAEFVGTFILIFAATAAPIINQKYNAALSLIGNAACSGLAVMIVIM 120
           T FP   VSLTRKLGAEFVGTFILIFAATA PI+NQKYN A +LIGNAAC+GLAVMIVI+
Sbjct: 61  TDFPVPDVSLTRKLGAEFVGTFILIFAATAGPIVNQKYNGAETLIGNAACAGLAVMIVIL 120

Query: 121 STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASYALKGVFHPFMSGGVT 180
           STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICAS+ALKGVFHPFMSGGVT
Sbjct: 121 STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASFALKGVFHPFMSGGVT 180

Query: 181 VPSVGTGQAFALEFLITFNLLFVVTAVATDTRAVGELAGIAVGATVLLNILIAGPSTGGS 240
           VPSV TGQAFALEF ITFNLLFVVTAVATDTRAVGELAGIAVGATV++NIL+AGPS+GGS
Sbjct: 181 VPSVSTGQAFALEFFITFNLLFVVTAVATDTRAVGELAGIAVGATVMVNILVAGPSSGGS 240

Query: 241 MNPVRTLGPAVAAGNYRELWVYMVAPTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR 292
           MNPVRTLGPAVAAGNYR LWVY+VAPTLGAIVGAGTY AVKL+DD+++ PS VRSFRR
Sbjct: 241 MNPVRTLGPAVAAGNYRALWVYLVAPTLGAIVGAGTYTAVKLRDDEVDAPSQVRSFRR 298

BLAST of Spg029261 vs. ExPASy TrEMBL
Match: A0A6J1K9S5 (probable aquaporin NIP5-1 OS=Cucurbita maxima OX=3661 GN=LOC111491380 PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 5.1e-125
Identity = 241/298 (80.87%), Postives = 261/298 (87.58%), Query Frame = 0

Query: 1   MSESESGILVSSRAESQ-------CSSLRVDSLSYDQKQMSQCECLPVNAPKRSQGHMCF 60
           M ESESG  V S   +         S LRVDSLSYD+K M +C+CLPVNAP   Q H CF
Sbjct: 1   MPESESGTPVPSAPATPGTPGGPLFSGLRVDSLSYDRKSMPRCKCLPVNAPTWGQPHTCF 60

Query: 61  THFPTLGVSLTRKLGAEFVGTFILIFAATAAPIINQKYNAALSLIGNAACSGLAVMIVIM 120
           T FP   VSLTRKLGAEFVGTFILIFAATA PI+NQKYN A +LIGNAAC+G+AVMIVI+
Sbjct: 61  TDFPVPDVSLTRKLGAEFVGTFILIFAATAGPIVNQKYNGAETLIGNAACAGMAVMIVIL 120

Query: 121 STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASYALKGVFHPFMSGGVT 180
           STGHISGAHLNPSLTIAFAALRHFPW+QVPAYIAAQVSASICAS+ALKGVFHPFMSGGVT
Sbjct: 121 STGHISGAHLNPSLTIAFAALRHFPWIQVPAYIAAQVSASICASFALKGVFHPFMSGGVT 180

Query: 181 VPSVGTGQAFALEFLITFNLLFVVTAVATDTRAVGELAGIAVGATVLLNILIAGPSTGGS 240
           VPSV TGQAFALEF ITFNLLFVVTAVATDTRAVGELAGIAVGATV++NIL+AGPS+GGS
Sbjct: 181 VPSVSTGQAFALEFFITFNLLFVVTAVATDTRAVGELAGIAVGATVMVNILVAGPSSGGS 240

Query: 241 MNPVRTLGPAVAAGNYRELWVYMVAPTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR 292
           MNPVRTLGPAVAAGNYR LWVY+VAPTLGAIVGAGTY AVKL+DD+++ PS VRSFRR
Sbjct: 241 MNPVRTLGPAVAAGNYRALWVYLVAPTLGAIVGAGTYTAVKLRDDEVDAPSQVRSFRR 298

BLAST of Spg029261 vs. ExPASy TrEMBL
Match: A0A0A0KQH1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G146190 PE=3 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.5e-124
Identity = 240/298 (80.54%), Postives = 261/298 (87.58%), Query Frame = 0

Query: 1   MSESESGILVSSRAESQ-------CSSLRVDSLSYDQKQMSQCECLPVNAPKRSQGHMCF 60
           M ESESG  V+S   +         S LRVDSLSYD+K M +C+CLPVNAP   Q H CF
Sbjct: 1   MPESESGTPVASAPATPGTPGGPLFSGLRVDSLSYDRKSMPRCKCLPVNAPTWGQPHTCF 60

Query: 61  THFPTLGVSLTRKLGAEFVGTFILIFAATAAPIINQKYNAALSLIGNAACSGLAVMIVIM 120
           T FP   VSLTRKLGAEFVGTFILIFAATA PI+NQKYN   +LIGNAAC+GLAVMIVI+
Sbjct: 61  TDFPAPDVSLTRKLGAEFVGTFILIFAATAGPIVNQKYNGVETLIGNAACAGLAVMIVIL 120

Query: 121 STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASYALKGVFHPFMSGGVT 180
           STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICAS+ALKGVFHPFMSGGVT
Sbjct: 121 STGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASFALKGVFHPFMSGGVT 180

Query: 181 VPSVGTGQAFALEFLITFNLLFVVTAVATDTRAVGELAGIAVGATVLLNILIAGPSTGGS 240
           VPSV  GQAFALEF+ITFNLLFVVTAVATDTRAVGELAGIAVGATV+LNIL+AGPS+GGS
Sbjct: 181 VPSVSIGQAFALEFIITFNLLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSSGGS 240

Query: 241 MNPVRTLGPAVAAGNYRELWVYMVAPTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR 292
           MNPVRTLGPAVAAGNY+ LWVY+VAPTLGAI+GAGTY AVKL+DD+++ PS VRSFRR
Sbjct: 241 MNPVRTLGPAVAAGNYKALWVYLVAPTLGAIIGAGTYTAVKLRDDEVDAPSQVRSFRR 298

BLAST of Spg029261 vs. ExPASy TrEMBL
Match: A0A2N9QK24 (NOD26-like intrinsic protein OS=Hevea brasiliensis OX=3981 GN=NIP5-1 PE=3 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 4.3e-124
Identity = 230/273 (84.25%), Postives = 252/273 (92.31%), Query Frame = 0

Query: 19  SSLRVDSLSYDQKQMSQCECLPVNAPKRSQGHMCFTHFPTLGVSLTRKLGAEFVGTFILI 78
           SSLRVDSLSYD+K M +C+CLPVNAP   Q H CFT FP+  VSLTRKLGAEFVGTFILI
Sbjct: 26  SSLRVDSLSYDRKSMPRCKCLPVNAPTWGQSHTCFTDFPSPDVSLTRKLGAEFVGTFILI 85

Query: 79  FAATAAPIINQKYNAALSLIGNAACSGLAVMIVIMSTGHISGAHLNPSLTIAFAALRHFP 138
           FAATA PI+NQK+N   SLIGNAAC+GLAVMI+I+STGHISGAHLNPSLTIAFAALRHFP
Sbjct: 86  FAATAGPIVNQKHNGVESLIGNAACAGLAVMIIILSTGHISGAHLNPSLTIAFAALRHFP 145

Query: 139 WVQVPAYIAAQVSASICASYALKGVFHPFMSGGVTVPSVGTGQAFALEFLITFNLLFVVT 198
           W+QVPAYIAAQVSASICAS+ALKGVFHPFMSGGVTVPSV TGQAFALEFLITFNLLFVVT
Sbjct: 146 WMQVPAYIAAQVSASICASFALKGVFHPFMSGGVTVPSVSTGQAFALEFLITFNLLFVVT 205

Query: 199 AVATDTRAVGELAGIAVGATVLLNILIAGPSTGGSMNPVRTLGPAVAAGNYRELWVYMVA 258
           AVATDTRAVGELAGIAVGATV+LNIL+AGPS+GGSMNPVRTLGPAVAAGNYR+LW+Y+VA
Sbjct: 206 AVATDTRAVGELAGIAVGATVMLNILVAGPSSGGSMNPVRTLGPAVAAGNYRDLWIYLVA 265

Query: 259 PTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR 292
           PTLGA+ GAGTY  VKL++D+ + P PVRSFRR
Sbjct: 266 PTLGALAGAGTYTLVKLREDEADPPRPVRSFRR 298

BLAST of Spg029261 vs. TAIR 10
Match: AT4G10380.1 (NOD26-like intrinsic protein 5;1 )

HSP 1 Score: 412.1 bits (1058), Expect = 3.6e-115
Identity = 214/274 (78.10%), Postives = 236/274 (86.13%), Query Frame = 0

Query: 19  SSLRVDSLSYD-QKQMSQCECLPVNAPKRSQGHMCFTHFPTLGVSLTRKLGAEFVGTFIL 78
           + +RVDS+S+D +K   +C+CLPV      Q   CFT FP+  VSLTRKLGAEFVGTFIL
Sbjct: 31  TGMRVDSMSFDHRKPTPRCKCLPVMGSTWGQHDTCFTDFPSPDVSLTRKLGAEFVGTFIL 90

Query: 79  IFAATAAPIINQKYNAALSLIGNAACSGLAVMIVIMSTGHISGAHLNPSLTIAFAALRHF 138
           IF ATA PI+NQKY+ A +LIGNAAC+GLAVMI+I+STGHISGAHLNPSLTIAFAALRHF
Sbjct: 91  IFTATAGPIVNQKYDGAETLIGNAACAGLAVMIIILSTGHISGAHLNPSLTIAFAALRHF 150

Query: 139 PWVQVPAYIAAQVSASICASYALKGVFHPFMSGGVTVPSVGTGQAFALEFLITFNLLFVV 198
           PW  VPAYIAAQVSASICAS+ALKGVFHPFMSGGVT+PSV  GQAFALEF+ITF LLFVV
Sbjct: 151 PWAHVPAYIAAQVSASICASFALKGVFHPFMSGGVTIPSVSLGQAFALEFIITFILLFVV 210

Query: 199 TAVATDTRAVGELAGIAVGATVLLNILIAGPSTGGSMNPVRTLGPAVAAGNYRELWVYMV 258
           TAVATDTRAVGELAGIAVGATV+LNIL+AGPSTGGSMNPVRTLGPAVA+GNYR LWVY+V
Sbjct: 211 TAVATDTRAVGELAGIAVGATVMLNILVAGPSTGGSMNPVRTLGPAVASGNYRSLWVYLV 270

Query: 259 APTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR 292
           APTLGAI GA  Y  VKL D   + P PVRSFRR
Sbjct: 271 APTLGAISGAAVYTGVKLNDSVTDPPRPVRSFRR 304

BLAST of Spg029261 vs. TAIR 10
Match: AT1G80760.1 (NOD26-like intrinsic protein 6;1 )

HSP 1 Score: 325.1 bits (832), Expect = 5.8e-89
Identity = 172/235 (73.19%), Postives = 196/235 (83.40%), Query Frame = 0

Query: 57  PTLGVSLTRKLGAEFVGTFILIFAATAAPIINQKYNAALSLIGNAACSGLAVMIVIMSTG 116
           P   VSL RKLGAEFVGT ILIFA TA  I+NQK + A +LIG AA +GLAVMIVI+STG
Sbjct: 72  PPPNVSLYRKLGAEFVGTLILIFAGTATAIVNQKTDGAETLIGCAASAGLAVMIVILSTG 131

Query: 117 HISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASYALKGVFHPFMSGGVTVPS 176
           HISGAHLNP++TIAFAAL+HFPW  VP YI AQV AS+ A++ALK VF P MSGGVTVP+
Sbjct: 132 HISGAHLNPAVTIAFAALKHFPWKHVPVYIGAQVMASVSAAFALKAVFEPTMSGGVTVPT 191

Query: 177 VGTGQAFALEFLITFNLLFVVTAVATDTRAVGELAGIAVGATVLLNILIAGPSTGGSMNP 236
           VG  QAFALEF+I+FNL+FVVTAVATDTRAVGELAGIAVGATV+LNILIAGP+T  SMNP
Sbjct: 192 VGLSQAFALEFIISFNLMFVVTAVATDTRAVGELAGIAVGATVMLNILIAGPATSASMNP 251

Query: 237 VRTLGPAVAAGNYRELWVYMVAPTLGAIVGAGTYAAVKLKDDKLEVPSPVRSFRR 292
           VRTLGPA+AA NYR +WVY+ AP LGA++GAGTY  VKL ++  E P   RSFRR
Sbjct: 252 VRTLGPAIAANNYRAIWVYLTAPILGALIGAGTYTIVKLPEED-EAPKERRSFRR 305

BLAST of Spg029261 vs. TAIR 10
Match: AT5G37820.1 (NOD26-like intrinsic protein 4;2 )

HSP 1 Score: 204.5 bits (519), Expect = 1.1e-52
Identity = 114/251 (45.42%), Postives = 158/251 (62.95%), Query Frame = 0

Query: 45  KRSQGHM--CFTHFPTLGVSLTRKLGAEFVGTFILIFAATAAPIINQKYNAALSLIGNAA 104
           K SQG M       P++ V LT+KL AE +GT+ +IF+     ++N  Y   ++  G   
Sbjct: 22  KDSQGGMETAICSSPSI-VCLTQKLIAEMIGTYFIIFSGCGVVVVNVLYGGTITFPGICV 81

Query: 105 CSGLAVMIVIMSTGHISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASYALKG 164
             GL VM++I STGHISGAH NP++T+ FA  R FPW QVP YI AQ++ S+ AS  L+ 
Sbjct: 82  TWGLIVMVMIYSTGHISGAHFNPAVTVTFAVFRRFPWYQVPLYIGAQLTGSLLASLTLRL 141

Query: 165 VFH--PFMSGGVTVPSVGTGQAFALEFLITFNLLFVVTAVATDTRAVGELAGIAVGATVL 224
           +F+  P    G T P+  +GQA   E +I+F L+FV++ VATD+RA GELAGIAVG T++
Sbjct: 142 MFNVTPKAFFG-TTPTDSSGQALVAEIIISFLLMFVISGVATDSRATGELAGIAVGMTII 201

Query: 225 LNILIAGPSTGGSMNPVRTLGPAVAAGNYRELWVYMVAPTLGAIVGAGTYAAVKLKDDKL 284
           LN+ +AGP +G SMNP R+LGPA+  G Y+ +WVY+V P +G   G   Y  ++  D  L
Sbjct: 202 LNVFVAGPISGASMNPARSLGPAIVMGRYKGIWVYIVGPFVGIFAGGFVYNFMRFTDKPL 261

Query: 285 EVPSPVRSFRR 292
              +   SF R
Sbjct: 262 RELTKSASFLR 270

BLAST of Spg029261 vs. TAIR 10
Match: AT1G31885.1 (NOD26-like intrinsic protein 3;1 )

HSP 1 Score: 203.0 bits (515), Expect = 3.3e-52
Identity = 110/226 (48.67%), Postives = 155/226 (68.58%), Query Frame = 0

Query: 57  PTLGVSLTRKLGAEFVGTFILIFAATAAPIINQKYNAALSLIGNAACSGLAVMIVIMSTG 116
           P + VS  +KL  EFVGTF +IFA  +A ++N+ Y   ++L G A   GL V ++I S G
Sbjct: 35  PLVSVSFVQKLIGEFVGTFTMIFAGCSAIVVNETYGKPVTLPGIALVWGLVVTVMIYSIG 94

Query: 117 HISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASYALKGVFH-----PFMSGG 176
           H+SGAH NP+++IAFA+ + FP+ QVP YIAAQ+  S  A+  L+ VFH       + G 
Sbjct: 95  HVSGAHFNPAVSIAFASSKKFPFNQVPGYIAAQLLGSTLAAAVLRLVFHLDDDVCSLKGD 154

Query: 177 V---TVPSVGTGQAFALEFLITFNLLFVVTAVATDTRAVGELAGIAVGATVLLNILIAGP 236
           V   T PS     +F +EF+ TFNL+FV++AVATD RA G  AGIA+GAT++L+IL +GP
Sbjct: 155 VYVGTYPSNSNTTSFVMEFIATFNLMFVISAVATDKRATGSFAGIAIGATIVLDILFSGP 214

Query: 237 STGGSMNPVRTLGPAVAAGNYRELWVYMVAPTLGAIVGAGTYAAVK 275
            +G SMNP R+LGPA+  G Y++LW+Y+V+P +GA+ GA TY  ++
Sbjct: 215 ISGASMNPARSLGPALIWGCYKDLWLYIVSPVIGALSGAWTYGLLR 260

BLAST of Spg029261 vs. TAIR 10
Match: AT4G19030.1 (NOD26-like major intrinsic protein 1 )

HSP 1 Score: 201.8 bits (512), Expect = 7.4e-52
Identity = 113/240 (47.08%), Postives = 160/240 (66.67%), Query Frame = 0

Query: 58  TLGVSLTRKLGAEFVGTFILIFAATAAPIINQKYNAALSLIGNAACSGLAVMIVIMSTGH 117
           ++ V   +KL AEF+GT+ L+F   A+ ++N + +  ++L G A   GL +M++I S GH
Sbjct: 48  SVSVPFLQKLIAEFLGTYFLVFTGCASVVVNMQNDNVVTLPGIAIVWGLTIMVLIYSLGH 107

Query: 118 ISGAHLNPSLTIAFAALRHFPWVQVPAYIAAQVSASICASYALK---GVFHPFMSG--GV 177
           ISGAH+NP++TIAFA+   FP  QVPAY+ +QV  S  A+  L+   G+ H   SG   V
Sbjct: 108 ISGAHINPAVTIAFASCGRFPLKQVPAYVISQVIGSTLAAATLRLLFGLDHDVCSGKHDV 167

Query: 178 TVPSVGTG---QAFALEFLITFNLLFVVTAVATDTRAVGELAGIAVGATVLLNILIAGPS 237
            + S   G   QAF +EF++TF L+F+++ VATD RA+GELAG+A+G+TVLLN+LIA P 
Sbjct: 168 FIGSSPVGSDLQAFTMEFIVTFYLMFIISGVATDNRAIGELAGLAIGSTVLLNVLIAAPV 227

Query: 238 TGGSMNPVRTLGPAVAAGNYRELWVYMVAPTLGAIVGAGTYAAVKLKDDKLEVPSPVRSF 290
           +  SMNP R+LGPA+  G Y+ +W+Y+VAPTLGAI GA  Y  V+  D  L   +   SF
Sbjct: 228 SSASMNPGRSLGPALVYGCYKGIWIYLVAPTLGAIAGAWVYNTVRYTDKPLREITKSGSF 287

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008437497.11.2e-12581.54PREDICTED: probable aquaporin NIP5-1 [Cucumis melo][more]
XP_038875525.12.8e-12581.54probable aquaporin NIP5-1 [Benincasa hispida][more]
XP_022957899.14.7e-12581.54probable aquaporin NIP5-1 [Cucurbita moschata] >XP_023534473.1 probable aquapori... [more]
XP_022996063.11.0e-12480.87probable aquaporin NIP5-1 [Cucurbita maxima][more]
XP_004145889.13.0e-12480.54probable aquaporin NIP5-1 [Cucumis sativus] >KGN49946.1 hypothetical protein Csa... [more]
Match NameE-valueIdentityDescription
Q9SV845.1e-11478.10Probable aquaporin NIP5-1 OS=Arabidopsis thaliana OX=3702 GN=NIP5-1 PE=2 SV=1[more]
Q0IWF33.2e-10873.72Aquaporin NIP3-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-1 PE=2 SV=2[more]
Q9ATN16.6e-10672.99Aquaporin NIP3-1 OS=Zea mays OX=4577 GN=NIP3-1 PE=2 SV=1[more]
Q9SAI48.1e-8873.19Aquaporin NIP6-1 OS=Arabidopsis thaliana OX=3702 GN=NIP6-1 PE=1 SV=1[more]
Q84S071.8e-5847.81Aquaporin NIP3-3 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP3-3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3AU646.0e-12681.54probable aquaporin NIP5-1 OS=Cucumis melo OX=3656 GN=LOC103482897 PE=3 SV=1[more]
A0A6J1H3D62.3e-12581.54probable aquaporin NIP5-1 OS=Cucurbita moschata OX=3662 GN=LOC111459297 PE=3 SV=... [more]
A0A6J1K9S55.1e-12580.87probable aquaporin NIP5-1 OS=Cucurbita maxima OX=3661 GN=LOC111491380 PE=3 SV=1[more]
A0A0A0KQH11.5e-12480.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G146190 PE=3 SV=1[more]
A0A2N9QK244.3e-12484.25NOD26-like intrinsic protein OS=Hevea brasiliensis OX=3981 GN=NIP5-1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G10380.13.6e-11578.10NOD26-like intrinsic protein 5;1 [more]
AT1G80760.15.8e-8973.19NOD26-like intrinsic protein 6;1 [more]
AT5G37820.11.1e-5245.42NOD26-like intrinsic protein 4;2 [more]
AT1G31885.13.3e-5248.67NOD26-like intrinsic protein 3;1 [more]
AT4G19030.17.4e-5247.08NOD26-like major intrinsic protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 253..273
score: 35.05
coord: 65..84
score: 34.64
coord: 141..160
score: 28.63
coord: 104..128
score: 42.82
coord: 216..238
score: 42.77
coord: 185..203
score: 30.68
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 62..270
e-value: 4.4E-50
score: 170.5
IPR000425Major intrinsic proteinCDDcd00333MIPcoord: 65..270
e-value: 3.30982E-53
score: 171.282
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 31..282
e-value: 3.6E-65
score: 221.4
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 61..276
IPR034294Aquaporin transporterPANTHERPTHR45724AQUAPORIN NIP2-1coord: 18..289
NoneNo IPR availablePANTHERPTHR45724:SF33AQUAPORIN NIP5-1 ISOFORM X1-RELATEDcoord: 18..289
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 122..130

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg029261.1Spg029261.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015267 channel activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity