Spg029226 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg029226
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	E3 ubiquitin-protein ligase RHA1B
Location	scaffold12: 36599203 .. 36601218 (+)
RNA-Seq Expression	Spg029226
Synteny	Spg029226

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCTTCCCTGCGAGCTACACCGAGCTTCTTCTTCCAAAGCTCTTCATCCATGTACTCTCCTTCCTCGGCTTCCTCAGGAAGCTCATCTCCCTCTTGTTTCGCCTTCTAGGGCTCCAGGATTTCCTCGAACCGGACATTCCTTGGCCCAATCAGCCCGATTCCTTCCCCACCGGGCCGTTGACGGACCTCGATCCTCTCTCCGCCGCCGTTCTCCGCGAGATTCTCCCCGTCGTGAAGTTTTCCGACCTTCTGGACCCGCCGGAGAGCTGCGCCGTGTGCCTCTACGAGTTCGAGGCCGACGACGAGATCCGCCGCCTCGCGAACTGCCGCCACATCTTCCACCGCGGTTGCCTCGATCGGTGGATCGGTTACGGCCAGAGGACCTGCCCGCTCTGCCGGACGGTGTTCATTCCGGCCGATTTGCGGACTGGCGGTTGTAATTTTGACGATAGGCTTTGGGAGGATTCGGAAATCTTCGAAGCACTCCACACCGATTCTTCCTCTTCTTCGTCGTCTTCGTCGAATCTTCTCACCGCTGGTTTGTAGCTTTTGGAGATTTGATTTTCTTTTGTTGATTTTGCTGCTTCTCATATCGGAAAACAGAAATAAAGAAGAAAAAGAAAAGAGAGAAAGAGAGGGGAAAGAGATGGAGATGATTGAGTTTGATGGAGGGGAAATGTTTGTTGTTCTTCTGTGTACATACCAAAAAAAGAAAAAGTTCCTGAAAGGAACTGGAAAATCAAAATCAAAATCAAATCAAATCAAATCAAATCAATTACAGGTTTCAGAATTAGATCAATCTTCTTGCAATTTTCATTTCCATTTCCTCTCTCTGTCTCTCTCTCTCTAACAATACAATTTCAAACAAATGGATTGGAAATTGAAACTGAAATTGAATCAATATGAGATTTAGATGTTGATGGATTTGGAGGAATGGAGATGAATGTTCTTGGAGATAAGAAGATTGTTGAAGAGTGGGGCAATGTTTTTGTAGCTCTGCCTGTTTTAATCCAATTGGTATAATTGGGAGATGCCCATTCATGTTTTTGAGTTCTTTGTTCTTTTTACTTTAATTTTATTTGAAGGAATTGAATTCTAAACTTCAATCAACTGTAAAATTAAGCCATGGGGATCATCCTTGTTCTTGAAGAAGATTGTTCTTTAGAAGAATTGGTTTGGATATTTTGTTTTTCCTTTTTACTTTGCTTGACTTTGTGTCTAGTTTTGAGAATTTTTTTGATTGAGTTTAGCTATTTTTATGGCTTAATTTTAAGTGGGTTTGTTTTTTCCAAAAAAAAAATAATAATAAATAATATTAATAAGTGGGTTTGTTGGTTGAATTTTGGGTTTTTTAAATTAAGAAAAAAGAATAACGCAAATGGAAAAAATTGGACAAAAGGACACAAACACAAAGAAACGCAATGAAATTTTGTGTTTAGTTGATGGACAATATTCTAGTGAATAGTATCTAGGTTTAGTTTCGGATTCGTGTAGTTTTAAAAATAATTGTTGTCAAATTCTTTTTTTTTTTTTTTTTTAAGTACAAGTGTTGATGTTCAAATTTGGTCGGAGGAACACCTTCACGTGCCACCAACGCGGTAAATTTGTTTGAGAGGATGGAAGACAGAAAACTTGTCCAAGAGGAGAAGTCGAGAATAGAGTCTGGGATCCCTTCTTCAGTGACGAAAAGGGGTTTAAATACCTGCTCATGTTCCTAGGGTTTTTAGGAATTCGGAGGTGATTCGGGTTGAACCGAGTGGAACCGAGGCAGCCGGGGGCACTAGTGACCGAACGGAGGTGGAGGAGCTCGGCCGAGGCCGAGCAGGGGGTCAGGCCAAAATCCCGATCCCTTCGGCTTTGGCCTGTTCTTCCTCTGGGTTCCGTTTCCTGACTGTCTCCTTAGGTTGGTATCGTGTTGTCCTCATTCAATTCCTCGCATATCGGAGTGGTCCAAAATTACCTATAACAACAAGAATATTTGGAGTTGGGAGATTCGAAACTTTGACTTCTTAG

mRNA sequence

ATGGGCTTCCCTGCGAGCTACACCGAGCTTCTTCTTCCAAAGCTCTTCATCCATGTACTCTCCTTCCTCGGCTTCCTCAGGAAGCTCATCTCCCTCTTGTTTCGCCTTCTAGGGCTCCAGGATTTCCTCGAACCGGACATTCCTTGGCCCAATCAGCCCGATTCCTTCCCCACCGGGCCGTTGACGGACCTCGATCCTCTCTCCGCCGCCGTTCTCCGCGAGATTCTCCCCGTCGTGAAGTTTTCCGACCTTCTGGACCCGCCGGAGAGCTGCGCCGTGTGCCTCTACGAGTTCGAGGCCGACGACGAGATCCGCCGCCTCGCGAACTGCCGCCACATCTTCCACCGCGGTTGCCTCGATCGGTGGATCGGTTACGGCCAGAGGACCTGCCCGCTCTGCCGGACGGTGTTCATTCCGGCCGATTTGCGGACTGGCGGTTGTAATTTTGACGATAGGCTTTGGGAGGATTCGGAAATCTTCGAAGCACTCCACACCGATTCTTCCTCTTCTTCGTCGTCTTCGTCGAATCTTCTCACCGCTGATGTTGATGGATTTGGAGGAATGGAGATGAATGTTCTTGGAGATAAGAAGATTGTTGAAGAGTGGGGCAATGTTTTTGTAGCTCTGCCTGTTTTAATCCAATTGGTGATTCGGGTTGAACCGAGTGGAACCGAGGCAGCCGGGGGCACTAGTGACCGAACGGAGGTGGAGGAGCTCGGCCGAGGCCGAGCAGGGGGTCAGGCCAAAATCCCGATCCCTTCGGCTTTGGCCTGTTCTTCCTCTGGGTTCCGTTTCCTGACTGTCTCCTTAGGTTGGTATCGTGTTGTCCTCATTCAATTCCTCGCATATCGGAGTGGTCCAAAATTACCTATAACAACAAGAATATTTGGAGTTGGGAGATTCGAAACTTTGACTTCTTAG

Coding sequence (CDS)

ATGGGCTTCCCTGCGAGCTACACCGAGCTTCTTCTTCCAAAGCTCTTCATCCATGTACTCTCCTTCCTCGGCTTCCTCAGGAAGCTCATCTCCCTCTTGTTTCGCCTTCTAGGGCTCCAGGATTTCCTCGAACCGGACATTCCTTGGCCCAATCAGCCCGATTCCTTCCCCACCGGGCCGTTGACGGACCTCGATCCTCTCTCCGCCGCCGTTCTCCGCGAGATTCTCCCCGTCGTGAAGTTTTCCGACCTTCTGGACCCGCCGGAGAGCTGCGCCGTGTGCCTCTACGAGTTCGAGGCCGACGACGAGATCCGCCGCCTCGCGAACTGCCGCCACATCTTCCACCGCGGTTGCCTCGATCGGTGGATCGGTTACGGCCAGAGGACCTGCCCGCTCTGCCGGACGGTGTTCATTCCGGCCGATTTGCGGACTGGCGGTTGTAATTTTGACGATAGGCTTTGGGAGGATTCGGAAATCTTCGAAGCACTCCACACCGATTCTTCCTCTTCTTCGTCGTCTTCGTCGAATCTTCTCACCGCTGATGTTGATGGATTTGGAGGAATGGAGATGAATGTTCTTGGAGATAAGAAGATTGTTGAAGAGTGGGGCAATGTTTTTGTAGCTCTGCCTGTTTTAATCCAATTGGTGATTCGGGTTGAACCGAGTGGAACCGAGGCAGCCGGGGGCACTAGTGACCGAACGGAGGTGGAGGAGCTCGGCCGAGGCCGAGCAGGGGGTCAGGCCAAAATCCCGATCCCTTCGGCTTTGGCCTGTTCTTCCTCTGGGTTCCGTTTCCTGACTGTCTCCTTAGGTTGGTATCGTGTTGTCCTCATTCAATTCCTCGCATATCGGAGTGGTCCAAAATTACCTATAACAACAAGAATATTTGGAGTTGGGAGATTCGAAACTTTGACTTCTTAG

Protein sequence

MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTGPLTDLDPLSAAVLREILPVVKFSDLLDPPESCAVCLYEFEADDEIRRLANCRHIFHRGCLDRWIGYGQRTCPLCRTVFIPADLRTGGCNFDDRLWEDSEIFEALHTDSSSSSSSSSNLLTADVDGFGGMEMNVLGDKKIVEEWGNVFVALPVLIQLVIRVEPSGTEAAGGTSDRTEVEELGRGRAGGQAKIPIPSALACSSSGFRFLTVSLGWYRVVLIQFLAYRSGPKLPITTRIFGVGRFETLTS

Homology

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BLAST of Spg029226 vs. NCBI nr
Match: XP_038906771.1 (E3 ubiquitin-protein ligase RHA1B [Benincasa hispida])

HSP 1 Score: 354.0 bits (907), Expect = 1.3e-93
Identity = 169/174 (97.13%), Postives = 171/174 (98.28%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTGP 60
           MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPN PDSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNHPDSFPTGP 60

Query: 61  LTDLDPLSAAVLREILPVVKFSDLLDPPESCAVCLYEFEADDEIRRLANCRHIFHRGCLD 120
            TDLDPLSAAVLREILPVVKFS+LLDPPESCAVCLYEFEADDEIRRLANCRHIFHRGCLD
Sbjct: 61  FTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFEADDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPADLRTGGCNFDDRLWEDSEIFEALHTDSSSSSSSS 175
           RWIGYGQRTCPLCRTVFIP DLR+GGCNFDDRLWEDSEIFEALHTDSSSSSSSS
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGCNFDDRLWEDSEIFEALHTDSSSSSSSS 174

[Close]

BLAST of Spg029226 vs. NCBI nr
Match: XP_023549736.1 (E3 ubiquitin-protein ligase RHA1B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 339.0 bits (868), Expect = 4.4e-89
Identity = 162/172 (94.19%), Postives = 167/172 (97.09%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTGP 60
           MGFPASYTELLLPKLFIHVLS LGFLRKLISLLFRLLGLQDFLEPDIPWPNQ DSF TGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSVLGFLRKLISLLFRLLGLQDFLEPDIPWPNQSDSFTTGP 60

Query: 61  LTDLDPLSAAVLREILPVVKFSDLLDPPESCAVCLYEFEADDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVVKFS+LLDPPESCAVCLYEFE+DDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPADLRTGGCNFDDRLWEDSEIFEALHTDSSSSSS 173
           RWIGYGQRTCPLCRTVFIP DLR+G CNFDDRLWEDSEIFEALH+DSSSSS+
Sbjct: 121 RWIGYGQRTCPLCRTVFIPGDLRSGVCNFDDRLWEDSEIFEALHSDSSSSST 172

[Close]

BLAST of Spg029226 vs. NCBI nr
Match: KAA0042739.1 (E3 ubiquitin-protein ligase RHA1B [Cucumis melo var. makuwa])

HSP 1 Score: 339.0 bits (868), Expect = 4.4e-89
Identity = 165/183 (90.16%), Postives = 171/183 (93.44%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTGP 60
           MGFPA+YTELLLPKLFIH+LSFLGFLRKLIS LFRL GLQDFLEPDIPWPN PDSFPT P
Sbjct: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60

Query: 61  LTDLDPLSAAVLREILPVVKFSDLLDPPESCAVCLYEFEADDEIRRLANCRHIFHRGCLD 120
            T LDPLSAA+LREILPVVKFSDLLDPP+ CAVCLYEFE+DDEIRRLANCRHIFHRGCLD
Sbjct: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPADLRT-GGCNFDDRLWEDSEIFEALHTD-SSSSSSSSSNLL 180
           RWIGYGQRTCPLCRTVFIP DLR+ GGCNFDDRLWEDSEIFE LHTD SSSSSSSSSNLL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGGCNFDDRLWEDSEIFERLHTDSSSSSSSSSSNLL 180

Query: 181 TAD 182
           T D
Sbjct: 181 TTD 183

[Close]

BLAST of Spg029226 vs. NCBI nr
Match: KAG6579522.1 (Brassinosteroid-responsive RING protein 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016982.1 E3 ubiquitin-protein ligase RHA1B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 338.6 bits (867), Expect = 5.7e-89
Identity = 163/177 (92.09%), Postives = 169/177 (95.48%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTGP 60
           MGFPASYTELLLPKLFIHVLS LGFLRKLISLLFRLLGLQDFLEPDIPWPNQ DSF TGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSVLGFLRKLISLLFRLLGLQDFLEPDIPWPNQSDSFTTGP 60

Query: 61  LTDLDPLSAAVLREILPVVKFSDLLDPPESCAVCLYEFEADDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVVKFS+LLDPPESCAVCLYEFE+DDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPADLRTGGCNFDDRLWEDSEIFEALHTDSSSSSSSSSNL 178
           RWIGYGQRTCPLCRTVFIP DLR+G CNFDDRLWEDSEIFEALH+DSSSS+  S+ L
Sbjct: 121 RWIGYGQRTCPLCRTVFIPGDLRSGVCNFDDRLWEDSEIFEALHSDSSSSTLLSAGL 177

[Close]

BLAST of Spg029226 vs. NCBI nr
Match: XP_022995549.1 (E3 ubiquitin-protein ligase RHA1B-like [Cucurbita maxima] >XP_023533861.1 E3 ubiquitin-protein ligase RHA1B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 338.6 bits (867), Expect = 5.7e-89
Identity = 161/172 (93.60%), Postives = 165/172 (95.93%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTGP 60
           MGFPASYTELLLPKLFIHVLS LGFLRKLISLLFRLLGLQDFLEPDIPW N  DSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFRLLGLQDFLEPDIPWSNHSDSFPTGP 60

Query: 61  LTDLDPLSAAVLREILPVVKFSDLLDPPESCAVCLYEFEADDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVV+FS+LLDPPESCAVCLYEFE DDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPADLRTGGCNFDDRLWEDSEIFEALHTDSSSSSS 173
           RWIGYGQRTCPLCRTVFIP DLRTGGCNFDDR WEDSE+FEALHTDSSSSS+
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSST 172

[Close]

BLAST of Spg029226 vs. ExPASy Swiss-Prot
Match: Q9XF92 (Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH1 PE=2 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.8e-45
Identity = 89/165 (53.94%), Postives = 113/165 (68.48%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTGP 60
           MGFP  YTE+ LPKLF+  LS LGF+R ++  +FR LGL DFLE D  WP+   S+PT  
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDY-TSYPTRI 60

Query: 61  LTDLDPLSAAVLREILPVVKFSDLL----DPPESCAVCLYEFEADDEIRRLANCRHIFHR 120
                P SA ++REILPV+KF +L     D PE+CAVCLYEFE + EIR L NCRHIFHR
Sbjct: 61  PETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHR 120

Query: 121 GCLDRWIGYGQRTCPLCRTVFIPADLRTGGCNFDDRLWEDSEIFE 162
            CLDRW+ + Q+TCPLCRT F+P +++     F+ RLW  S + +
Sbjct: 121 SCLDRWMDHDQKTCPLCRTPFVPDEMQE---EFNQRLWAASGVHD 161

[Close]

BLAST of Spg029226 vs. ExPASy Swiss-Prot
Match: Q9SUS5 (E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 5.6e-23
Identity = 57/146 (39.04%), Postives = 81/146 (55.48%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTGP 60
           MG P  + EL +P   +  L  +GF R ++  L   +GL  FL+ +    + P       
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLDHNETSRSDPTRLALS- 60

Query: 61  LTDLDPLSAAVLREILPVVKFSDLL-DPPESCAVCLYEFEADDEIRRLANCRHIFHRGCL 120
                  SA +  E++PVV+FSDLL DP + C VCL +F +DD+IR+L  C H+FH  CL
Sbjct: 61  ------TSATLANELIPVVRFSDLLTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCL 120

Query: 121 DRWI-GYGQRTCPLCRTVFIPADLRT 145
           DRWI    + TCP+CR  F+P +  T
Sbjct: 121 DRWIVDCNKITCPICRNRFLPEEKST 139

[Close]

BLAST of Spg029226 vs. ExPASy Swiss-Prot
Match: Q9SUS4 (Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RHA1A PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 2.1e-22
Identity = 60/147 (40.82%), Postives = 85/147 (57.82%), Query Frame = 0


Query: 1   MGFPASY-TELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTG 60
           MG P  + TEL +P   + +L  +GF R ++  L   +GL  FL  D    + PD     
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFL--DHNETSAPDLTRHA 60

Query: 61  PLTDLDPLSAAVLREILPVVKFSDL-LDPPESCAVCLYEFEADDEIRRLANCRHIFHRGC 120
             T     SA++  E++PVV+FSDL  DP + C VCL +FE+DD++R+L  C H+FH  C
Sbjct: 61  LST-----SASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYC 120

Query: 121 LDRWI-GYGQRTCPLCRTVFIPADLRT 145
           LDRWI  Y +  CP+CR  F+P +  T
Sbjct: 121 LDRWIVDYNKMKCPVCRHRFLPKEKYT 140

[Close]

BLAST of Spg029226 vs. ExPASy Swiss-Prot
Match: Q9LZJ6 (RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 2.2e-11
Identity = 48/127 (37.80%), Postives = 63/127 (49.61%), Query Frame = 0


Query: 57  PTGPLTDLDPLSAAVLREILPVVKFSDLL--DPPESCAVCLYEFEADDEIRRLANCRHIF 116
           PT   + L PL   VL +I P+  +S      P E C+VCL EFE DDE R L  C H+F
Sbjct: 78  PTQSSSSLSPLDPTVLEKI-PIFVYSVKTHESPLEECSVCLSEFEEDDEGRVLPKCGHVF 137

Query: 117 HRGCLDRWIGYGQRTCPLCRTVFIPADLRT-----GGCNFDDRLWEDSEIFEALHTDSSS 176
           H  C+D W    + +CPLCR    PA   T            +  ED+E   +  +D S 
Sbjct: 138 HVDCIDTWF-RSRSSCPLCRAPVQPAQPVTEPEPVAAVFPSVKPIEDTEAGSSSSSDESE 197

[Close]

BLAST of Spg029226 vs. ExPASy Swiss-Prot
Match: Q9P7E1 (Uncharacterized RING finger protein P4H10.07 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBP4H10.07 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.9e-11
Identity = 27/47 (57.45%), Postives = 32/47 (68.09%), Query Frame = 0


Query: 89  ESCAVCLYEFEADDEIRRLANCRHIFHRGCLDRWIGYGQRTCPLCRT 136
           E C VCL  FE +DE RRL  C H FHR C+D+W+   Q +CPLCRT
Sbjct: 523 ERCLVCLSNFELNDECRRLKQCNHFFHRECIDQWLTSSQNSCPLCRT 569

[Close]

BLAST of Spg029226 vs. ExPASy TrEMBL
Match: A0A5A7TMZ8 (E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002380 PE=4 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 2.1e-89
Identity = 165/183 (90.16%), Postives = 171/183 (93.44%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTGP 60
           MGFPA+YTELLLPKLFIH+LSFLGFLRKLIS LFRL GLQDFLEPDIPWPN PDSFPT P
Sbjct: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPPDSFPTSP 60

Query: 61  LTDLDPLSAAVLREILPVVKFSDLLDPPESCAVCLYEFEADDEIRRLANCRHIFHRGCLD 120
            T LDPLSAA+LREILPVVKFSDLLDPP+ CAVCLYEFE+DDEIRRLANCRHIFHRGCLD
Sbjct: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPADLRT-GGCNFDDRLWEDSEIFEALHTD-SSSSSSSSSNLL 180
           RWIGYGQRTCPLCRTVFIP DLR+ GGCNFDDRLWEDSEIFE LHTD SSSSSSSSSNLL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGGCNFDDRLWEDSEIFERLHTDSSSSSSSSSSNLL 180

Query: 181 TAD 182
           T D
Sbjct: 181 TTD 183

[Close]

BLAST of Spg029226 vs. ExPASy TrEMBL
Match: A0A6J1K664 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita maxima OX=3661 GN=LOC111491048 PE=4 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 2.8e-89
Identity = 161/172 (93.60%), Postives = 165/172 (95.93%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTGP 60
           MGFPASYTELLLPKLFIHVLS LGFLRKLISLLFRLLGLQDFLEPDIPW N  DSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFRLLGLQDFLEPDIPWSNHSDSFPTGP 60

Query: 61  LTDLDPLSAAVLREILPVVKFSDLLDPPESCAVCLYEFEADDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVV+FS+LLDPPESCAVCLYEFE DDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPADLRTGGCNFDDRLWEDSEIFEALHTDSSSSSS 173
           RWIGYGQRTCPLCRTVFIP DLRTGGCNFDDR WEDSE+FEALHTDSSSSS+
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSST 172

[Close]

BLAST of Spg029226 vs. ExPASy TrEMBL
Match: A0A6J1I1X7 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita maxima OX=3661 GN=LOC111469101 PE=4 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 8.0e-89
Identity = 162/177 (91.53%), Postives = 168/177 (94.92%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTGP 60
           MGFPASYTELLLPKLFIHVLS LGFLRKLISLLFRLLGLQDFLEPDIPWPNQ DSF TGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSILGFLRKLISLLFRLLGLQDFLEPDIPWPNQSDSFTTGP 60

Query: 61  LTDLDPLSAAVLREILPVVKFSDLLDPPESCAVCLYEFEADDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVVKFS+LLDPPESCAVCLYEFE+DDEIR LANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFESDDEIRHLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPADLRTGGCNFDDRLWEDSEIFEALHTDSSSSSSSSSNL 178
           RWIGYGQRTCPLCRTVFIP DLR+G CNFDDRLWEDSEIFEALH+DSSSS+  S+ L
Sbjct: 121 RWIGYGQRTCPLCRTVFIPGDLRSGVCNFDDRLWEDSEIFEALHSDSSSSTLLSAGL 177

[Close]

BLAST of Spg029226 vs. ExPASy TrEMBL
Match: A0A6J1H5J7 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC111459775 PE=4 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 1.4e-88
Identity = 160/172 (93.02%), Postives = 164/172 (95.35%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTGP 60
           MGFPASYTELLLPKLFIHVLS LGFLRKLISLLF LLGLQDFLEPDIPW N  DSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60

Query: 61  LTDLDPLSAAVLREILPVVKFSDLLDPPESCAVCLYEFEADDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVV+FS+LLDPPESCAVCLYEFE DDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPADLRTGGCNFDDRLWEDSEIFEALHTDSSSSSS 173
           RWIGYGQRTCPLCRTVFIP DLRTGGCNFDDR WEDSE+FEALHTDSSSSS+
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRAWEDSELFEALHTDSSSSST 172

[Close]

BLAST of Spg029226 vs. ExPASy TrEMBL
Match: A0A1S3AU97 (E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103482771 PE=4 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.8e-88
Identity = 164/183 (89.62%), Postives = 170/183 (92.90%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTGP 60
           MGFPA+YTELLLPKLFIH+LSFLGFLRKLIS LFRL GLQDFLEPDIPWPN  DSFPT P
Sbjct: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPSDSFPTSP 60

Query: 61  LTDLDPLSAAVLREILPVVKFSDLLDPPESCAVCLYEFEADDEIRRLANCRHIFHRGCLD 120
            T LDPLSAA+LREILPVVKFSDLLDPP+ CAVCLYEFE+DDEIRRLANCRHIFHRGCLD
Sbjct: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPADLRT-GGCNFDDRLWEDSEIFEALHTD-SSSSSSSSSNLL 180
           RWIGYGQRTCPLCRTVFIP DLR+ GGCNFDDRLWEDSEIFE LHTD SSSSSSSSSNLL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGGCNFDDRLWEDSEIFEPLHTDSSSSSSSSSSNLL 180

Query: 181 TAD 182
           T D
Sbjct: 181 TTD 183

[Close]

BLAST of Spg029226 vs. TAIR 10
Match: AT1G63840.1 (RING/U-box superfamily protein )

HSP 1 Score: 188.0 bits (476), Expect = 1.2e-47
Identity = 103/173 (59.54%), Postives = 123/173 (71.10%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTGP 60
           MGFP  Y+ELLLPK+F ++LSFLG +RKLIS +F+++GL DFLEP+    + PD  PT  
Sbjct: 1   MGFPVGYSELLLPKIFFYLLSFLGLIRKLISTMFKIIGLPDFLEPEPVSTSWPDPPPT-- 60

Query: 61  LTDLDPLSAAVLR-EILPVVKFSDLLDP-PESCAVCLYEFEADDEIRRLANCRHIFHRGC 120
           LT   P SAA+L  E+LPVV+FSD+  P  E CAVCLY+FE DDEIRRL NCRHIFHRGC
Sbjct: 61  LT--KPDSAAILAGEMLPVVRFSDINRPESECCAVCLYDFENDDEIRRLTNCRHIFHRGC 120

Query: 121 LDRW-IGYGQRTCPLCRTVFIPADLRTGGCNFDDRLWEDSEIFEALHTDSSSS 171
           LDRW +GY Q TCPLCRT FIP  L+     F+ RLW  S    +   D SSS
Sbjct: 121 LDRWMMGYNQMTCPLCRTQFIPDHLQ---LEFNQRLWSQSSAVSSQLLDESSS 166

[Close]

BLAST of Spg029226 vs. TAIR 10
Match: AT3G61460.1 (brassinosteroid-responsive RING-H2 )

HSP 1 Score: 184.5 bits (467), Expect = 1.3e-46
Identity = 89/165 (53.94%), Postives = 113/165 (68.48%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTGP 60
           MGFP  YTE+ LPKLF+  LS LGF+R ++  +FR LGL DFLE D  WP+   S+PT  
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDY-TSYPTRI 60

Query: 61  LTDLDPLSAAVLREILPVVKFSDLL----DPPESCAVCLYEFEADDEIRRLANCRHIFHR 120
                P SA ++REILPV+KF +L     D PE+CAVCLYEFE + EIR L NCRHIFHR
Sbjct: 61  PETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHR 120

Query: 121 GCLDRWIGYGQRTCPLCRTVFIPADLRTGGCNFDDRLWEDSEIFE 162
            CLDRW+ + Q+TCPLCRT F+P +++     F+ RLW  S + +
Sbjct: 121 SCLDRWMDHDQKTCPLCRTPFVPDEMQE---EFNQRLWAASGVHD 161

[Close]

BLAST of Spg029226 vs. TAIR 10
Match: AT5G41400.1 (RING/U-box superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 2.3e-40
Identity = 87/174 (50.00%), Postives = 111/174 (63.79%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPD-----IPWPNQPDS 60
           MG+P  YTELLLP++F+H+LS LG +R LI   FR+LGL DFLE D       W   P  
Sbjct: 1   MGYPVGYTELLLPRIFLHLLSLLGLIRTLIDTGFRILGLPDFLESDPVSSSSSWLEPPYM 60

Query: 61  FPTG----PLTDLDPLSAAVLREILPVVKFSDLLDP-----PESCAVCLYEFEADDEIRR 120
                     +   P++A +  EILPV++FS+L  P      + CAVCL+EFE DDEIRR
Sbjct: 61  STAAHHHQESSFFFPVAARLAGEILPVIRFSELTRPGFGSGSDCCAVCLHEFENDDEIRR 120

Query: 121 LANCRHIFHRGCLDRW-IGYGQRTCPLCRTVFIPADLRTGGCNFDDRLWEDSEI 160
           L NC+HIFHR CLDRW +GY Q TCPLCRT FI  +L+     F+ R+W +SE+
Sbjct: 121 LTNCQHIFHRSCLDRWMMGYNQMTCPLCRTPFISDELQVA---FNQRVWSESEL 171

[Close]

BLAST of Spg029226 vs. TAIR 10
Match: AT4G11360.1 (RING-H2 finger A1B )

HSP 1 Score: 109.8 bits (273), Expect = 4.0e-24
Identity = 57/146 (39.04%), Postives = 81/146 (55.48%), Query Frame = 0


Query: 1   MGFPASYTELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTGP 60
           MG P  + EL +P   +  L  +GF R ++  L   +GL  FL+ +    + P       
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLDHNETSRSDPTRLALS- 60

Query: 61  LTDLDPLSAAVLREILPVVKFSDLL-DPPESCAVCLYEFEADDEIRRLANCRHIFHRGCL 120
                  SA +  E++PVV+FSDLL DP + C VCL +F +DD+IR+L  C H+FH  CL
Sbjct: 61  ------TSATLANELIPVVRFSDLLTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCL 120

Query: 121 DRWI-GYGQRTCPLCRTVFIPADLRT 145
           DRWI    + TCP+CR  F+P +  T
Sbjct: 121 DRWIVDCNKITCPICRNRFLPEEKST 139

[Close]

BLAST of Spg029226 vs. TAIR 10
Match: AT4G11370.1 (RING-H2 finger A1A )

HSP 1 Score: 107.8 bits (268), Expect = 1.5e-23
Identity = 60/147 (40.82%), Postives = 85/147 (57.82%), Query Frame = 0


Query: 1   MGFPASY-TELLLPKLFIHVLSFLGFLRKLISLLFRLLGLQDFLEPDIPWPNQPDSFPTG 60
           MG P  + TEL +P   + +L  +GF R ++  L   +GL  FL  D    + PD     
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFL--DHNETSAPDLTRHA 60

Query: 61  PLTDLDPLSAAVLREILPVVKFSDL-LDPPESCAVCLYEFEADDEIRRLANCRHIFHRGC 120
             T     SA++  E++PVV+FSDL  DP + C VCL +FE+DD++R+L  C H+FH  C
Sbjct: 61  LST-----SASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYC 120

Query: 121 LDRWI-GYGQRTCPLCRTVFIPADLRT 145
           LDRWI  Y +  CP+CR  F+P +  T
Sbjct: 121 LDRWIVDYNKMKCPVCRHRFLPKEKYT 140

The following BLAST results are available for this feature:

BLAST of Spg029226 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906771.1	1.3e-93	97.13	E3 ubiquitin-protein ligase RHA1B [Benincasa hispida]	[more]
XP_023549736.1	4.4e-89	94.19	E3 ubiquitin-protein ligase RHA1B [Cucurbita pepo subsp. pepo]	[more]
KAA0042739.1	4.4e-89	90.16	E3 ubiquitin-protein ligase RHA1B [Cucumis melo var. makuwa]	[more]
KAG6579522.1	5.7e-89	92.09	Brassinosteroid-responsive RING protein 1, partial [Cucurbita argyrosperma subsp...	[more]
XP_022995549.1	5.7e-89	93.60	E3 ubiquitin-protein ligase RHA1B-like [Cucurbita maxima] >XP_023533861.1 E3 ubi...	[more]

BLAST of Spg029226 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XF92	1.8e-45	53.94	Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH...	[more]
Q9SUS5	5.6e-23	39.04	E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 ...	[more]
Q9SUS4	2.1e-22	40.82	Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q9LZJ6	2.2e-11	37.80	RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1	[more]
Q9P7E1	2.9e-11	57.45	Uncharacterized RING finger protein P4H10.07 OS=Schizosaccharomyces pombe (strai...	[more]

BLAST of Spg029226 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TMZ8	2.1e-89	90.16	E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A6J1K664	2.8e-89	93.60	E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita maxima OX=3661 GN=LOC1114910...	[more]
A0A6J1I1X7	8.0e-89	91.53	E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita maxima OX=3661 GN=LOC1114691...	[more]
A0A6J1H5J7	1.4e-88	93.02	E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A1S3AU97	1.8e-88	89.62	E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103482771 PE=4 S...	[more]

BLAST of Spg029226 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G63840.1	1.2e-47	59.54	RING/U-box superfamily protein	[more]
AT3G61460.1	1.3e-46	53.94	brassinosteroid-responsive RING-H2	[more]
AT5G41400.1	2.3e-40	50.00	RING/U-box superfamily protein	[more]
AT4G11360.1	4.0e-24	39.04	RING-H2 finger A1B	[more]
AT4G11370.1	1.5e-23	40.82	RING-H2 finger A1A	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 90..134 e-value: 0.0082 score: 7.9
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 91..133 e-value: 2.9E-6 score: 36.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 90..134 e-value: 3.6E-13 score: 49.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 91..134 score: 12.386007
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 85..137 e-value: 2.3E-17 score: 64.3
None	No IPR available	PANTHER	PTHR45969	RING ZINC FINGER PROTEIN-RELATED	coord: 1..157
None	No IPR available	PANTHER	PTHR45969:SF46	BNAA09G11240D PROTEIN	coord: 1..157
None	No IPR available	CDD	cd16448	RING-H2	coord: 91..134 e-value: 4.81876E-15 score: 65.9363
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 75..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg029226.1	Spg029226.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding