Spg029220 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg029220 vs. NCBI nr
Match: KAG6579613.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 521.9 bits (1343), Expect = 3.9e-144
Identity = 275/362 (75.97%), Postives = 295/362 (81.49%), Query Frame = 0

Query: 1   MTKLYASCGAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRV 60
           MTKLYASC AMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAF++LAPFAYV+DR 
Sbjct: 1   MTKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDR- 60

Query: 61  NDSPQSVLCRN----------------RLYFADGGFG----------------------F 120
            + P S+                     L++   G+                       F
Sbjct: 61  -NKPASLTISTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLF 120

Query: 121 QLEKLKIRTARGAAKVVGTILCIGGSLIFTFWKGPYVTKAIFKEPLIDIYKNQDSGSGTG 180
           +LEK+ IRTARGAAKVVGTILCIGGSLIFT WKGPYVTKAIFKEPLIDIYKN DSG+ +G
Sbjct: 121 RLEKVNIRTARGAAKVVGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYKNHDSGNSSG 180

Query: 181 KAWIKGSAFILISDIAWSAWLILQAFVTRKYPAQLTITVLICFFATAQSGFLALIFARSP 240
           K WIKGSAFILISDIAWS WLILQAFVTRKYPAQLT+TVLIC FATAQSGFLALIFAR P
Sbjct: 181 KDWIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALIFARDP 240

Query: 241 DKWKLQWDARLLTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAF 300
           +KWKLQWDARLLTIVY+GIVIS VGYFLQ+W IS NGPVFTSMFSPL+LIFVAIFSAF F
Sbjct: 241 EKWKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVF 300

Query: 301 SERLHVGSLVGAFFIVLGLYLVLWGKKADHMVAVEPQENDKVFVDTEMQNTSVNDNRVKD 325
           SERLHVGSLVGAFFIVLGLYLVLWGKKADH+VA EPQE DKVFVDTEMQNTSVND++VK 
Sbjct: 301 SERLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQNTSVNDSQVKG 360

BLAST of Spg029220 vs. NCBI nr
Match: XP_022928958.1 (WAT1-related protein At1g43650-like [Cucurbita moschata])

HSP 1 Score: 518.1 bits (1333), Expect = 5.6e-143
Identity = 273/362 (75.41%), Postives = 294/362 (81.22%), Query Frame = 0

Query: 1   MTKLYASCGAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRV 60
           M KLYASC AMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAF++LAPFAYV+DR 
Sbjct: 1   MAKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDR- 60

Query: 61  NDSPQSVLCRN----------------RLYFADGGFG----------------------F 120
            + P S+                     L++   G+                       F
Sbjct: 61  -NKPASLTISTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLF 120

Query: 121 QLEKLKIRTARGAAKVVGTILCIGGSLIFTFWKGPYVTKAIFKEPLIDIYKNQDSGSGTG 180
           +LEK+ IRTARGAAKVVGTILCIGGSLIFT WKGPYVTKAIFKEPLIDIYKN DSG+ +G
Sbjct: 121 RLEKVNIRTARGAAKVVGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYKNHDSGNSSG 180

Query: 181 KAWIKGSAFILISDIAWSAWLILQAFVTRKYPAQLTITVLICFFATAQSGFLALIFARSP 240
           K WIKGSAFILISDIAWS WLILQAFVTRKYPAQLT+TVLIC FATAQSGFLALIFAR P
Sbjct: 181 KDWIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALIFARDP 240

Query: 241 DKWKLQWDARLLTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAF 300
           +KWKLQWDARLLTIVY+GIVIS VGYFLQ+W IS NGPVFTSMFSPL+LIFVAIFSAF F
Sbjct: 241 EKWKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVF 300

Query: 301 SERLHVGSLVGAFFIVLGLYLVLWGKKADHMVAVEPQENDKVFVDTEMQNTSVNDNRVKD 325
           SERLHVGSLVGAFFIVLGLYLVLWGKKADH+VA EPQE DKVFVDTEMQ+TSVND++VK 
Sbjct: 301 SERLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQSTSVNDSQVKG 360

BLAST of Spg029220 vs. NCBI nr
Match: XP_022970063.1 (WAT1-related protein At1g43650-like [Cucurbita maxima])

HSP 1 Score: 517.3 bits (1331), Expect = 9.6e-143
Identity = 272/362 (75.14%), Postives = 293/362 (80.94%), Query Frame = 0

Query: 1   MTKLYASCGAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRV 60
           MTKLYASC AMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAF++LAPFAYV+DR 
Sbjct: 1   MTKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDR- 60

Query: 61  NDSPQSVLCRN----------------RLYFADGGFG----------------------F 120
            + P S+                     L++   G+                       F
Sbjct: 61  -NKPASLTISTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLF 120

Query: 121 QLEKLKIRTARGAAKVVGTILCIGGSLIFTFWKGPYVTKAIFKEPLIDIYKNQDSGSGTG 180
           +LEK+ IRTARGAAKV GTILCIGGSLIFT WKGPYVTKAIFKEPLIDIY+N DSG+ +G
Sbjct: 121 RLEKVNIRTARGAAKVAGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYRNHDSGNSSG 180

Query: 181 KAWIKGSAFILISDIAWSAWLILQAFVTRKYPAQLTITVLICFFATAQSGFLALIFARSP 240
           K WIKGSAFILISDIAWS WLILQAFVTRKYPAQLT+TVLIC FATAQSGFLALIFAR P
Sbjct: 181 KDWIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALIFARDP 240

Query: 241 DKWKLQWDARLLTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAF 300
           +KWKLQWDARLLTIVY+GIVIS VGYFLQ+W IS NGPVFTSMFSPL+LIFVAIFSAF F
Sbjct: 241 EKWKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVF 300

Query: 301 SERLHVGSLVGAFFIVLGLYLVLWGKKADHMVAVEPQENDKVFVDTEMQNTSVNDNRVKD 325
           SERLHVGSLVGAFFIVLGLYLVLWGKKADH+VA EPQE DKVFVDTEMQNTSVND++VK 
Sbjct: 301 SERLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQNTSVNDSQVKG 360

BLAST of Spg029220 vs. NCBI nr
Match: XP_023520560.1 (WAT1-related protein At1g43650-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 514.2 bits (1323), Expect = 8.1e-142
Identity = 267/360 (74.17%), Postives = 292/360 (81.11%), Query Frame = 0

Query: 1   MTKLYASCGAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRV 60
           MTKLYASC AMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAF++LAPFAY +DR 
Sbjct: 1   MTKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYFIDRN 60

Query: 61  NDSPQSV--------------LCRNRLYFADGGFG----------------------FQL 120
             +  ++                   L++   G+                       F+L
Sbjct: 61  KPASLTIPTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRL 120

Query: 121 EKLKIRTARGAAKVVGTILCIGGSLIFTFWKGPYVTKAIFKEPLIDIYKNQDSGSGTGKA 180
           EK+ IR+A G AKV+GTI+CIGGSLIFT WKGPYVTK +FKEPLIDIYKN DSG+ +GK 
Sbjct: 121 EKVNIRSAGGGAKVLGTIVCIGGSLIFTLWKGPYVTKGMFKEPLIDIYKNHDSGNSSGKD 180

Query: 181 WIKGSAFILISDIAWSAWLILQAFVTRKYPAQLTITVLICFFATAQSGFLALIFARSPDK 240
           WIKGSAFILISDIAWS WLILQAFVTRKYPAQLT+TVLICFFATAQSGFLALIFAR P+K
Sbjct: 181 WIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICFFATAQSGFLALIFARDPEK 240

Query: 241 WKLQWDARLLTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAFSE 300
           WKLQWDARLLTIVY+GIVIS VGYFLQ+W ISHNGPVFTSMFSPL+LIFVAIFSAF FSE
Sbjct: 241 WKLQWDARLLTIVYSGIVISGVGYFLQTWCISHNGPVFTSMFSPLMLIFVAIFSAFVFSE 300

Query: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHMVAVEPQENDKVFVDTEMQNTSVNDNRVKDSV 325
           RLHVGSLVGAFFIVLGLYLVLWGKKADH+VA EPQE DKVFVDTEMQNTSVN ++VKDSV
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQNTSVNHSQVKDSV 360

BLAST of Spg029220 vs. NCBI nr
Match: XP_023521473.1 (WAT1-related protein At1g43650-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 510.8 bits (1314), Expect = 9.0e-141
Identity = 267/360 (74.17%), Postives = 289/360 (80.28%), Query Frame = 0

Query: 1   MTKLYASCGAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRV 60
           M KLYASC AMV VQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAF++LAPFAY +DR 
Sbjct: 1   MAKLYASCAAMVAVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYFIDRN 60

Query: 61  NDSPQSV--------------LCRNRLYFADGGFG----------------------FQL 120
             +  ++                   L++   G+                       F+L
Sbjct: 61  KPASLTIPTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRL 120

Query: 121 EKLKIRTARGAAKVVGTILCIGGSLIFTFWKGPYVTKAIFKEPLIDIYKNQDSGSGTGKA 180
           EK+ IR+A G AKV+GTILCIGGSLIFT WKGPYVTKAIFKEPLIDIYKN DSG+ +GK 
Sbjct: 121 EKVNIRSAGGGAKVLGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYKNHDSGNSSGKD 180

Query: 181 WIKGSAFILISDIAWSAWLILQAFVTRKYPAQLTITVLICFFATAQSGFLALIFARSPDK 240
           WIKGSAFILISDIAWS WLILQAFVTRKYPAQLT+TVLICFFATAQSGFLALIFAR P K
Sbjct: 181 WIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICFFATAQSGFLALIFARDPQK 240

Query: 241 WKLQWDARLLTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAFSE 300
           WKLQWDARLLTIVY+GIVIS VGYFLQ+W IS NGPVFTSMFSPL+LIFVAIFSAF FSE
Sbjct: 241 WKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVFSE 300

Query: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHMVAVEPQENDKVFVDTEMQNTSVNDNRVKDSV 325
           RLHVGSLVGAFFIVLGLYLVLWGKKADH+VA EPQE DKVFVDTEMQNTSVN ++VKDSV
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQNTSVNHSQVKDSV 360

BLAST of Spg029220 vs. ExPASy Swiss-Prot
Match: Q9LPF1 (WAT1-related protein At1g44800 OS=Arabidopsis thaliana OX=3702 GN=At1g44800 PE=1 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.7e-47
Identity = 112/342 (32.75%), Postives = 176/342 (51.46%), Query Frame = 0

Query: 10  AMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRVNDSPQSV-- 69
           A++ +Q GY G  I+  ++ +  ++ +V   YRH +A VV+APFA + +R      ++  
Sbjct: 14  AIISLQFGYAGMYIITMVSFKHGMDHWVLATYRHVVATVVMAPFALMFERKIRPKMTLAI 73

Query: 70  ------------LCRNRLYFA---------DGGFG-------------FQLEKLKIRTAR 129
                       L    LY+             F              F+LE +  R   
Sbjct: 74  FWRLLALGILEPLMDQNLYYIGLKNTSASYTSAFTNALPAVTFILALIFRLETVNFRKVH 133

Query: 130 GAAKVVGTILCIGGSLIFTFWKGPYVTKAIFKEPLIDIYKNQDSGSGTGKAWIKGSAFIL 189
             AKVVGT++ +GG++I T +KGP +      +   + +    S + TG+ W+ G+  I+
Sbjct: 134 SVAKVVGTVITVGGAMIMTLYKGPAIE---IVKAAHNSFHGGSSSTPTGQHWVLGTIAIM 193

Query: 190 ISDIAWSAWLILQAFVTRKYPAQLTITVLICFFATAQSGFLALIFARSPDKWKLQWDARL 249
            S   W+A+ ILQ++  + YPA+L++  LIC   T  +   +LI  R P  WK+  D+  
Sbjct: 194 GSISTWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAIASLIMVRDPSAWKIGMDSGT 253

Query: 250 LTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAFSERLHVGSLVG 309
           L  VY+G+V S + Y++QS VI   GPVFT+ FSP+ +I  A   A   +E++H+GS++G
Sbjct: 254 LAAVYSGVVCSGIAYYIQSIVIKQRGPVFTTSFSPMCMIITAFLGALVLAEKIHLGSIIG 313

Query: 310 AFFIVLGLYLVLWGKKADHMVAVEPQENDKVFVDTEMQNTSV 316
           A FIVLGLY V+WGK  D    V P +   V    E+  T+V
Sbjct: 314 AVFIVLGLYSVVWGKSKDE---VNPLDEKIVAKSQELPITNV 349

BLAST of Spg029220 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 6.0e-47
Identity = 110/364 (30.22%), Postives = 185/364 (50.82%), Query Frame = 0

Query: 10  AMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRVNDSPQSVLC 69
           AM+ +Q GY G NI+ KI+    ++ +V VVYRH IA  V+APFA+  +R    P+    
Sbjct: 21  AMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFER-KAQPKITFS 80

Query: 70  RNRLYFADGGFG-------------------------------------FQLEKLKIRTA 129
                F  G  G                                     F++E L ++  
Sbjct: 81  IFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKL 140

Query: 130 RGAAKVVGTILCIGGSLIFTFWKGPYV----TKAIFKEPLIDIYKNQDSGSGTGKAWIKG 189
              AK+ GT++ + G+++ T +KGP V    TK +  +            S + K ++KG
Sbjct: 141 WCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFLKG 200

Query: 190 SAFILISDIAWSAWLILQAFVTRKYPA-QLTITVLICFFATAQSGFLALIFARSPDKWKL 249
           S  ++ + +AW++  +LQA + + Y   QL++T LICF  T Q+  +  +   +P  W++
Sbjct: 201 SILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAWRI 260

Query: 250 QWDARLLTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAFSERLH 309
            WD  LL   Y+GIV S++ Y++Q  V+   GPVF + FSPL+++ VA+  +F  +E++ 
Sbjct: 261 GWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIF 320

Query: 310 VGSLVGAFFIVLGLYLVLWGKKADHMVAV----EPQENDKVFVDTEMQNTSVNDNRVKDS 328
           +G ++GA  IV+GLY VLWGK+ ++ V +    +   N KV  D E   + +  +   +S
Sbjct: 321 LGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTEDVEANGSKMKISEGDNS 380

BLAST of Spg029220 vs. ExPASy Swiss-Prot
Match: F4IQX1 (WAT1-related protein At2g37450 OS=Arabidopsis thaliana OX=3702 GN=At2g37450 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 7.9e-47
Identity = 118/335 (35.22%), Postives = 178/335 (53.13%), Query Frame = 0

Query: 11  MVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRVNDSPQSVLCR 70
           MV++QIGY G +IL K    K ++ +V  VYRH +A VV+APFA+  D  N      L  
Sbjct: 13  MVLLQIGYAGMDILTKDVLNKGMSIYVLSVYRHGVATVVMAPFAFYFD--NPVIAQNLFN 72

Query: 71  NRLYFADGGFG-----------------FQLEKLKIRTARGAAKVVGTILCIGGSLIFTF 130
             + +    F                  F+LE +K ++ R AAKVVGT+  +GG ++ T 
Sbjct: 73  LGMKYTTATFAIALYNTLPAVTFILALIFRLESVKFQSIRSAAKVVGTVTTVGGIMVMTL 132

Query: 131 WKGPYVTKAIFKEPLIDIYKNQDSGSGTGKAWIKGSAFILISDIAWSAWLILQAFVTRKY 190
            KGP +     K P        D  S      IKG+  + I   +++ ++ILQA   + Y
Sbjct: 133 VKGPALDLFWTKGPSAQNTVGTDIHSS-----IKGAVLVTIGCFSYACFMILQAITLKTY 192

Query: 191 PAQLTITVLICFFATAQSGFLALIFAR-SPDKWKLQWDARLLTIVYNGIVISAVGYFLQS 250
           PA+L++   IC   T +   +AL+  + +P  W + WD +LLTI Y+GIV SA+GY++  
Sbjct: 193 PAELSLATWICLIGTIEGVVVALVMEKGNPSVWAIGWDTKLLTITYSGIVCSALGYYIGG 252

Query: 251 WVISHNGPVFTSMFSPLLLIFVAIFSAFAFSERLHVGSLVGAFFIVLGLYLVLWGKKADH 310
            V+   GPVF + F PL +I VAI S+  F E++++G  +GA  I +GLYLV+WGK  D+
Sbjct: 253 VVMKTRGPVFVTAFKPLCMIVVAIMSSIIFDEQMYLGRALGATVICVGLYLVIWGKAKDY 312

Query: 311 MVAVEPQENDKVFVDTEMQNTSVNDNRVKDSVIET 328
                PQ +D +      Q T+      + +VIE+
Sbjct: 313 EYPSTPQIDDDL-----AQATTSKQKEQRRTVIES 335

BLAST of Spg029220 vs. ExPASy Swiss-Prot
Match: Q501F8 (WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.3e-46
Identity = 101/326 (30.98%), Postives = 171/326 (52.45%), Query Frame = 0

Query: 10  AMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDR---------- 69
           A++ +Q GY G  I+  ++ +  +N ++   YRH +A +V+APFA +L+R          
Sbjct: 14  AIISLQFGYAGMYIITMVSFKHGMNHWILATYRHVVATIVIAPFALILERKIRPKMTWPL 73

Query: 70  -----VNDSPQSVLCRNRLYFADGGFG---------------------FQLEKLKIRTAR 129
                     + +L +N  Y                            F++E + ++  R
Sbjct: 74  FLRILALGFLEPLLDQNLYYIGMKATSATYSSAFVNALPAITFIMAVIFRIETVNLKKTR 133

Query: 130 GAAKVVGTILCIGGSLIFTFWKGPYVTKAIFKEPLIDIY--KNQDSGSGTGKAWIKGSAF 189
             AKV+GT + +GG+++ T +KGP +   +FK     ++   +  S   T + W+ G+  
Sbjct: 134 SLAKVIGTAITVGGAMVMTLYKGPAI--ELFKTAHSSLHGGSSGTSSETTDQNWVTGTLA 193

Query: 190 ILISDIAWSAWLILQAFVTRKYPAQLTITVLICFFATAQSGFLALIFARSPDKWKLQWDA 249
           ++ S   W+ + ILQ+F  +KYPA+L++ + IC   T  +   +LI  R    WK+  D+
Sbjct: 194 VMGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIASLIMVRDVSAWKVGMDS 253

Query: 250 RLLTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAFSERLHVGSL 298
             L  VY+G+V S + Y++QS VI   GPVFT+ FSP+ +I  A       +E++H+GS+
Sbjct: 254 GTLAAVYSGVVCSGMAYYIQSIVIRERGPVFTTSFSPMCMIITAFLGVLVLAEKIHLGSI 313

BLAST of Spg029220 vs. ExPASy Swiss-Prot
Match: Q6NMB7 (WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 5.1e-46
Identity = 111/324 (34.26%), Postives = 171/324 (52.78%), Query Frame = 0

Query: 10  AMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRVNDSPQSVLC 69
           AMV VQI Y G  +L K+A  +  NPFVFV YR   A + L+PFA+ L+    SP S + 
Sbjct: 10  AMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSKSSPLSFIL 69

Query: 70  RNRLYF-------------------ADGGFG-----------------FQLEKLKIRTAR 129
             +++F                       F                  F+LE + ++ + 
Sbjct: 70  LLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLETVTLKKSH 129

Query: 130 GAAKVVGTILCIGGSLIFTFWKGPYVTKAIFKEPLIDIYKNQDSGSGT---GKAWIKGSA 189
           G AKV G+++ + G+L+F F KGP          LI+ Y +    +GT    K  +KGS 
Sbjct: 130 GVAKVTGSMVGMLGALVFAFVKGP---------SLINHYNSSTIPNGTVPSTKNSVKGSI 189

Query: 190 FILISDIAWSAWLILQAFVTRKYPAQLTITVLICFFATAQSGFLALIFARSPDKWKLQWD 249
            +L ++  W  W+I+Q+ V ++YPA+L +  L C F+  QS   A+   R+P  WK+++ 
Sbjct: 190 TMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWKIEFG 249

Query: 250 ARLLTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAFSERLHVGS 295
             LL++ Y GI+++ + Y+LQ W I   GPVFT++++PL LI   I S+F F E  ++GS
Sbjct: 250 LPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETFYLGS 309

BLAST of Spg029220 vs. ExPASy TrEMBL
Match: A0A6J1EQL7 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435703 PE=3 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 2.7e-143
Identity = 273/362 (75.41%), Postives = 294/362 (81.22%), Query Frame = 0

Query: 1   MTKLYASCGAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRV 60
           M KLYASC AMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAF++LAPFAYV+DR 
Sbjct: 1   MAKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDR- 60

Query: 61  NDSPQSVLCRN----------------RLYFADGGFG----------------------F 120
            + P S+                     L++   G+                       F
Sbjct: 61  -NKPASLTISTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLF 120

Query: 121 QLEKLKIRTARGAAKVVGTILCIGGSLIFTFWKGPYVTKAIFKEPLIDIYKNQDSGSGTG 180
           +LEK+ IRTARGAAKVVGTILCIGGSLIFT WKGPYVTKAIFKEPLIDIYKN DSG+ +G
Sbjct: 121 RLEKVNIRTARGAAKVVGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYKNHDSGNSSG 180

Query: 181 KAWIKGSAFILISDIAWSAWLILQAFVTRKYPAQLTITVLICFFATAQSGFLALIFARSP 240
           K WIKGSAFILISDIAWS WLILQAFVTRKYPAQLT+TVLIC FATAQSGFLALIFAR P
Sbjct: 181 KDWIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALIFARDP 240

Query: 241 DKWKLQWDARLLTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAF 300
           +KWKLQWDARLLTIVY+GIVIS VGYFLQ+W IS NGPVFTSMFSPL+LIFVAIFSAF F
Sbjct: 241 EKWKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVF 300

Query: 301 SERLHVGSLVGAFFIVLGLYLVLWGKKADHMVAVEPQENDKVFVDTEMQNTSVNDNRVKD 325
           SERLHVGSLVGAFFIVLGLYLVLWGKKADH+VA EPQE DKVFVDTEMQ+TSVND++VK 
Sbjct: 301 SERLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQSTSVNDSQVKG 360

BLAST of Spg029220 vs. ExPASy TrEMBL
Match: A0A6J1I2R9 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469064 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 4.7e-143
Identity = 272/362 (75.14%), Postives = 293/362 (80.94%), Query Frame = 0

Query: 1   MTKLYASCGAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRV 60
           MTKLYASC AMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAF++LAPFAYV+DR 
Sbjct: 1   MTKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDR- 60

Query: 61  NDSPQSVLCRN----------------RLYFADGGFG----------------------F 120
            + P S+                     L++   G+                       F
Sbjct: 61  -NKPASLTISTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLF 120

Query: 121 QLEKLKIRTARGAAKVVGTILCIGGSLIFTFWKGPYVTKAIFKEPLIDIYKNQDSGSGTG 180
           +LEK+ IRTARGAAKV GTILCIGGSLIFT WKGPYVTKAIFKEPLIDIY+N DSG+ +G
Sbjct: 121 RLEKVNIRTARGAAKVAGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYRNHDSGNSSG 180

Query: 181 KAWIKGSAFILISDIAWSAWLILQAFVTRKYPAQLTITVLICFFATAQSGFLALIFARSP 240
           K WIKGSAFILISDIAWS WLILQAFVTRKYPAQLT+TVLIC FATAQSGFLALIFAR P
Sbjct: 181 KDWIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALIFARDP 240

Query: 241 DKWKLQWDARLLTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAF 300
           +KWKLQWDARLLTIVY+GIVIS VGYFLQ+W IS NGPVFTSMFSPL+LIFVAIFSAF F
Sbjct: 241 EKWKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVF 300

Query: 301 SERLHVGSLVGAFFIVLGLYLVLWGKKADHMVAVEPQENDKVFVDTEMQNTSVNDNRVKD 325
           SERLHVGSLVGAFFIVLGLYLVLWGKKADH+VA EPQE DKVFVDTEMQNTSVND++VK 
Sbjct: 301 SERLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQNTSVNDSQVKG 360

BLAST of Spg029220 vs. ExPASy TrEMBL
Match: A0A6J1HY24 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469060 PE=3 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 1.3e-137
Identity = 255/364 (70.05%), Postives = 292/364 (80.22%), Query Frame = 0

Query: 1   MTKLYASCGAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRV 60
           M KLYASC AMV VQIGYGGSNILMK+ASEKDL+PFVF VYRH IAF+VL PFAYV+DR 
Sbjct: 1   MAKLYASCAAMVAVQIGYGGSNILMKVASEKDLDPFVFFVYRHIIAFLVLLPFAYVIDRN 60

Query: 61  NDSPQSVLCRNRLYF-----------------------ADGGFG-------------FQL 120
             +  ++    +++                            F              F+L
Sbjct: 61  KQTSLTISTTIKIFLLAILGSTTYLNLFYLGIKYTSATVASAFSNVTPSFTFVMAVLFRL 120

Query: 121 EKLKIRTARGAAKVVGTILCIGGSLIFTFWKGPYVTKAIFKEPLIDIYKNQDSGSGTGKA 180
           EK+ I+TARGAAKV+GT++CIGGSLIFTFWKGPY+TKAIFKEPLI+IYKNQDSG+ +GK 
Sbjct: 121 EKVNIKTARGAAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKD 180

Query: 181 WIKGSAFILISDIAWSAWLILQAFVTRKYPAQLTITVLICFFATAQSGFLALIFARSPDK 240
           WIKGSAFI++S+++WSAWLILQ FVTRKYPA+LTITVL C FATAQSGFLAL FAR P+K
Sbjct: 181 WIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEK 240

Query: 241 WKLQWDARLLTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAFSE 300
           WKL WDARLLTIVY+GI+ISAVGYF+Q+WVISHNGPVFTS+F+PL+LIFVAIFSAF FSE
Sbjct: 241 WKLHWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSE 300

Query: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHMVAVEPQENDKVFVDTEMQNTSVNDNRVKDSV 329
           RLHVGSLVGAFFIVLGLYLVLWGKKADH+VA E QE +KVFVDTEMQ+TSVNDN VKDSV
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFVDTEMQSTSVNDNEVKDSV 360

BLAST of Spg029220 vs. ExPASy TrEMBL
Match: A0A6J1ESW6 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435701 PE=3 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 3.2e-136
Identity = 253/364 (69.51%), Postives = 290/364 (79.67%), Query Frame = 0

Query: 1   MTKLYASCGAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRV 60
           M KLYASC AMV VQIGYGGSNILMK+ASEKDL+PFVF VYRH IAF+VL PFAYV+DR 
Sbjct: 1   MAKLYASCAAMVAVQIGYGGSNILMKVASEKDLDPFVFFVYRHIIAFLVLLPFAYVIDRN 60

Query: 61  NDSPQSVLCRNRLYF-----------------------ADGGFG-------------FQL 120
             +  ++    +++                            F              F+L
Sbjct: 61  KRASLTISMTIKIFLLAILGSTTYLNLFYLGIKYTSATVASAFSNVTPSFTFVMAVLFRL 120

Query: 121 EKLKIRTARGAAKVVGTILCIGGSLIFTFWKGPYVTKAIFKEPLIDIYKNQDSGSGTGKA 180
           EK+ I+TARG AKV+GT++CIGGSLIFTFWKGPY+TKAIFKEPLI+IYKNQDSG+ +GK 
Sbjct: 121 EKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKD 180

Query: 181 WIKGSAFILISDIAWSAWLILQAFVTRKYPAQLTITVLICFFATAQSGFLALIFARSPDK 240
           WIKGSAFI++S+++WSAWLILQ FVTRKYPA+LTITVL C FATAQSGFLAL FAR P+K
Sbjct: 181 WIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEK 240

Query: 241 WKLQWDARLLTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAFSE 300
           WKLQWDARLLTIVY+GI+ISAVGYF+Q+WVISHNGPVFTS+F+PL+LIFVAIFSAF FSE
Sbjct: 241 WKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSE 300

Query: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHMVAVEPQENDKVFVDTEMQNTSVNDNRVKDSV 329
           RLHVGSLVGAFFIVLGLYLVLWGKKADH+VA E QE +KVF DTEMQ+TSVNDN VKDS 
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVNDNEVKDSE 360

BLAST of Spg029220 vs. ExPASy TrEMBL
Match: A0A6J1EQM5 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435707 PE=3 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 1.6e-114
Identity = 218/334 (65.27%), Postives = 248/334 (74.25%), Query Frame = 0

Query: 1   MTKLYASCGAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRV 60
           M KLY  C AMV+V I YGGS+IL+KIAS K LNP VF+VYRHFIAF+VLAPFAY++DR 
Sbjct: 8   MRKLYGPCAAMVLVYIAYGGSHILVKIASNKGLNPLVFIVYRHFIAFLVLAPFAYLIDRN 67

Query: 61  NDSPQSVLCRNRLY-------------FADG-----------------------GFGFQL 120
             +  ++     ++             F  G                        F F+L
Sbjct: 68  KPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFNNIIPSLTFVMAFLFRL 127

Query: 121 EKLKIRTARGAAKVVGTILCIGGSLIFTFWKGPYVTKAIFKEPLIDIYKNQDSGSGTGKA 180
           E++ IRTARG AKV+GTI CIGGS +FTFWKGPYVTK IFKEPLID+YKNQ  G  +G+ 
Sbjct: 128 ERVNIRTARGGAKVLGTITCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSGED 187

Query: 181 WIKGSAFILISDIAWSAWLILQAFVTRKYPAQLTITVLICFFATAQSGFLALIFARSPDK 240
           WIKGSA I++S++AWSAWLIL   VTRKYPAQLTITVLIC FATAQSGFLALIFAR  +K
Sbjct: 188 WIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEK 247

Query: 241 WKLQWDARLLTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAFSE 299
           WKL WDARLLTIVY G+VIS  GYFLQ+W IS NGPVFTSMF+PL+LIFVAIFSAF FSE
Sbjct: 248 WKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSE 307

BLAST of Spg029220 vs. TAIR 10
Match: AT1G44800.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 190.7 bits (483), Expect = 1.9e-48
Identity = 112/342 (32.75%), Postives = 176/342 (51.46%), Query Frame = 0

Query: 10  AMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRVNDSPQSV-- 69
           A++ +Q GY G  I+  ++ +  ++ +V   YRH +A VV+APFA + +R      ++  
Sbjct: 14  AIISLQFGYAGMYIITMVSFKHGMDHWVLATYRHVVATVVMAPFALMFERKIRPKMTLAI 73

Query: 70  ------------LCRNRLYFA---------DGGFG-------------FQLEKLKIRTAR 129
                       L    LY+             F              F+LE +  R   
Sbjct: 74  FWRLLALGILEPLMDQNLYYIGLKNTSASYTSAFTNALPAVTFILALIFRLETVNFRKVH 133

Query: 130 GAAKVVGTILCIGGSLIFTFWKGPYVTKAIFKEPLIDIYKNQDSGSGTGKAWIKGSAFIL 189
             AKVVGT++ +GG++I T +KGP +      +   + +    S + TG+ W+ G+  I+
Sbjct: 134 SVAKVVGTVITVGGAMIMTLYKGPAIE---IVKAAHNSFHGGSSSTPTGQHWVLGTIAIM 193

Query: 190 ISDIAWSAWLILQAFVTRKYPAQLTITVLICFFATAQSGFLALIFARSPDKWKLQWDARL 249
            S   W+A+ ILQ++  + YPA+L++  LIC   T  +   +LI  R P  WK+  D+  
Sbjct: 194 GSISTWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAIASLIMVRDPSAWKIGMDSGT 253

Query: 250 LTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAFSERLHVGSLVG 309
           L  VY+G+V S + Y++QS VI   GPVFT+ FSP+ +I  A   A   +E++H+GS++G
Sbjct: 254 LAAVYSGVVCSGIAYYIQSIVIKQRGPVFTTSFSPMCMIITAFLGALVLAEKIHLGSIIG 313

Query: 310 AFFIVLGLYLVLWGKKADHMVAVEPQENDKVFVDTEMQNTSV 316
           A FIVLGLY V+WGK  D    V P +   V    E+  T+V
Sbjct: 314 AVFIVLGLYSVVWGKSKDE---VNPLDEKIVAKSQELPITNV 349

BLAST of Spg029220 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 189.5 bits (480), Expect = 4.3e-48
Identity = 110/364 (30.22%), Postives = 185/364 (50.82%), Query Frame = 0

Query: 10  AMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRVNDSPQSVLC 69
           AM+ +Q GY G NI+ KI+    ++ +V VVYRH IA  V+APFA+  +R    P+    
Sbjct: 21  AMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFER-KAQPKITFS 80

Query: 70  RNRLYFADGGFG-------------------------------------FQLEKLKIRTA 129
                F  G  G                                     F++E L ++  
Sbjct: 81  IFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKL 140

Query: 130 RGAAKVVGTILCIGGSLIFTFWKGPYV----TKAIFKEPLIDIYKNQDSGSGTGKAWIKG 189
              AK+ GT++ + G+++ T +KGP V    TK +  +            S + K ++KG
Sbjct: 141 WCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFLKG 200

Query: 190 SAFILISDIAWSAWLILQAFVTRKYPA-QLTITVLICFFATAQSGFLALIFARSPDKWKL 249
           S  ++ + +AW++  +LQA + + Y   QL++T LICF  T Q+  +  +   +P  W++
Sbjct: 201 SILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAWRI 260

Query: 250 QWDARLLTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAFSERLH 309
            WD  LL   Y+GIV S++ Y++Q  V+   GPVF + FSPL+++ VA+  +F  +E++ 
Sbjct: 261 GWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIF 320

Query: 310 VGSLVGAFFIVLGLYLVLWGKKADHMVAV----EPQENDKVFVDTEMQNTSVNDNRVKDS 328
           +G ++GA  IV+GLY VLWGK+ ++ V +    +   N KV  D E   + +  +   +S
Sbjct: 321 LGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTEDVEANGSKMKISEGDNS 380

BLAST of Spg029220 vs. TAIR 10
Match: AT2G37450.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 189.1 bits (479), Expect = 5.6e-48
Identity = 118/335 (35.22%), Postives = 178/335 (53.13%), Query Frame = 0

Query: 11  MVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRVNDSPQSVLCR 70
           MV++QIGY G +IL K    K ++ +V  VYRH +A VV+APFA+  D  N      L  
Sbjct: 13  MVLLQIGYAGMDILTKDVLNKGMSIYVLSVYRHGVATVVMAPFAFYFD--NPVIAQNLFN 72

Query: 71  NRLYFADGGFG-----------------FQLEKLKIRTARGAAKVVGTILCIGGSLIFTF 130
             + +    F                  F+LE +K ++ R AAKVVGT+  +GG ++ T 
Sbjct: 73  LGMKYTTATFAIALYNTLPAVTFILALIFRLESVKFQSIRSAAKVVGTVTTVGGIMVMTL 132

Query: 131 WKGPYVTKAIFKEPLIDIYKNQDSGSGTGKAWIKGSAFILISDIAWSAWLILQAFVTRKY 190
            KGP +     K P        D  S      IKG+  + I   +++ ++ILQA   + Y
Sbjct: 133 VKGPALDLFWTKGPSAQNTVGTDIHSS-----IKGAVLVTIGCFSYACFMILQAITLKTY 192

Query: 191 PAQLTITVLICFFATAQSGFLALIFAR-SPDKWKLQWDARLLTIVYNGIVISAVGYFLQS 250
           PA+L++   IC   T +   +AL+  + +P  W + WD +LLTI Y+GIV SA+GY++  
Sbjct: 193 PAELSLATWICLIGTIEGVVVALVMEKGNPSVWAIGWDTKLLTITYSGIVCSALGYYIGG 252

Query: 251 WVISHNGPVFTSMFSPLLLIFVAIFSAFAFSERLHVGSLVGAFFIVLGLYLVLWGKKADH 310
            V+   GPVF + F PL +I VAI S+  F E++++G  +GA  I +GLYLV+WGK  D+
Sbjct: 253 VVMKTRGPVFVTAFKPLCMIVVAIMSSIIFDEQMYLGRALGATVICVGLYLVIWGKAKDY 312

Query: 311 MVAVEPQENDKVFVDTEMQNTSVNDNRVKDSVIET 328
                PQ +D +      Q T+      + +VIE+
Sbjct: 313 EYPSTPQIDDDL-----AQATTSKQKEQRRTVIES 335

BLAST of Spg029220 vs. TAIR 10
Match: AT2G37450.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 188.3 bits (477), Expect = 9.5e-48
Identity = 114/318 (35.85%), Postives = 172/318 (54.09%), Query Frame = 0

Query: 11  MVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDRVNDSPQSVLCR 70
           MV++QIGY G +IL K    K ++ +V  VYRH +A VV+APFA+  D+           
Sbjct: 13  MVLLQIGYAGMDILTKDVLNKGMSIYVLSVYRHGVATVVMAPFAFYFDK----------- 72

Query: 71  NRLYFADGGFGFQLEKLKIRTARGAAKVVGTILCIGGSLIFTFWKGPYVTKAIFKEPLID 130
                        LE +K ++ R AAKVVGT+  +GG ++ T  KGP +     K P   
Sbjct: 73  -------------LESVKFQSIRSAAKVVGTVTTVGGIMVMTLVKGPALDLFWTKGPSAQ 132

Query: 131 IYKNQDSGSGTGKAWIKGSAFILISDIAWSAWLILQAFVTRKYPAQLTITVLICFFATAQ 190
                D  S      IKG+  + I   +++ ++ILQA   + YPA+L++   IC   T +
Sbjct: 133 NTVGTDIHSS-----IKGAVLVTIGCFSYACFMILQAITLKTYPAELSLATWICLIGTIE 192

Query: 191 SGFLALIFAR-SPDKWKLQWDARLLTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPL 250
              +AL+  + +P  W + WD +LLTI Y+GIV SA+GY++   V+   GPVF + F PL
Sbjct: 193 GVVVALVMEKGNPSVWAIGWDTKLLTITYSGIVCSALGYYIGGVVMKTRGPVFVTAFKPL 252

Query: 251 LLIFVAIFSAFAFSERLHVGSLVGAFFIVLGLYLVLWGKKADHMVAVEPQENDKVFVDTE 310
            +I VAI S+  F E++++G  +GA  I +GLYLV+WGK  D+     PQ +D +     
Sbjct: 253 CMIVVAIMSSIIFDEQMYLGRALGATVICVGLYLVIWGKAKDYEYPSTPQIDDDL----- 296

Query: 311 MQNTSVNDNRVKDSVIET 328
            Q T+      + +VIE+
Sbjct: 313 AQATTSKQKEQRRTVIES 296

BLAST of Spg029220 vs. TAIR 10
Match: AT4G08300.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 187.6 bits (475), Expect = 1.6e-47
Identity = 101/326 (30.98%), Postives = 171/326 (52.45%), Query Frame = 0

Query: 10  AMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFVVLAPFAYVLDR---------- 69
           A++ +Q GY G  I+  ++ +  +N ++   YRH +A +V+APFA +L+R          
Sbjct: 14  AIISLQFGYAGMYIITMVSFKHGMNHWILATYRHVVATIVIAPFALILERKIRPKMTWPL 73

Query: 70  -----VNDSPQSVLCRNRLYFADGGFG---------------------FQLEKLKIRTAR 129
                     + +L +N  Y                            F++E + ++  R
Sbjct: 74  FLRILALGFLEPLLDQNLYYIGMKATSATYSSAFVNALPAITFIMAVIFRIETVNLKKTR 133

Query: 130 GAAKVVGTILCIGGSLIFTFWKGPYVTKAIFKEPLIDIY--KNQDSGSGTGKAWIKGSAF 189
             AKV+GT + +GG+++ T +KGP +   +FK     ++   +  S   T + W+ G+  
Sbjct: 134 SLAKVIGTAITVGGAMVMTLYKGPAI--ELFKTAHSSLHGGSSGTSSETTDQNWVTGTLA 193

Query: 190 ILISDIAWSAWLILQAFVTRKYPAQLTITVLICFFATAQSGFLALIFARSPDKWKLQWDA 249
           ++ S   W+ + ILQ+F  +KYPA+L++ + IC   T  +   +LI  R    WK+  D+
Sbjct: 194 VMGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIASLIMVRDVSAWKVGMDS 253

Query: 250 RLLTIVYNGIVISAVGYFLQSWVISHNGPVFTSMFSPLLLIFVAIFSAFAFSERLHVGSL 298
             L  VY+G+V S + Y++QS VI   GPVFT+ FSP+ +I  A       +E++H+GS+
Sbjct: 254 GTLAAVYSGVVCSGMAYYIQSIVIRERGPVFTTSFSPMCMIITAFLGVLVLAEKIHLGSI 313