Spg029067 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg029067
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionUlp1-like peptidase
Locationscaffold11: 22550491 .. 22553767 (-)
RNA-Seq ExpressionSpg029067
SyntenySpg029067
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATCCCGGTCTCGATTTCGACCGAGATTTAGACCGGGAATTATGGCAGAGGGCTTGTTTTTAGTTAATTTTTTTTTTTTATTTGTATATTTGAAAATGTTGATTATGAATTGTTGTAATTTGCTAGGTCTAGTATGGAAATCACCGAGAAAATCCCCTCGCTGACCATTTTCCGACTGCGTTGACATGTTGCTCACACCTAAACAAAACCATTAGCAATATTAAGTCAACCGTAAGTCGTACCCAATTAGACCTCTTTAGGCAAACATGTTTCGGGCCTTTAATAGATGTGAATGTTATTTTTAATGGCCAATTAGTACACTACATCCTCCTTAGGGAAGTAGAGGAGAATAGGGCAAATGTGATGAGTTTTAAATTGTTAGGTCAGAAGGTCTCCTTTGGTAAGAGAGATTTTGACCTCATAACCGGCCTTCGTCATTCATTTAGACAAATGAGGAGAGATAGAGAGGGCCCTCCCAATAGACTCCTAAGATTATATTTTAGGGAGAATGTAGGCATGAAGGTGGAGGAGTTAGATAAGTCGTTTCCGACCCTTCAGTTTGAGAACGACAAAGATGCAGTTAAGATCGTAGCGTTTTATTTTTTTGAGTTGGCTTTGATGGGGAGGGAACGCAAACAACAAGTAGATGCCAGCACCCTAGACTTAATGGATGATTGGGTGGCATTCTGCAATGAGGATTGGAGTACCATCGTTTTCGACAAGACGATCAAAAGTTTGAAGAAGGCACTAAAGGGGAAGGCTGAGTCGTACAAGCGTAAGGGAAGTGGTCCAAAGAAGCAGGAGACATACAGTCTATACGGTTTCCCGTTTGCTTTTCAGGTTAGTAGATAATTAAACTTTTGAAATGTAATCTTCATATAGTTTGTATGGTTTCCCATTTTTTTTCTTTAATTGTTTCTTTACACAGATATGGGCATACGAGACTGTTTCATCTCTCACTGGACGTGTTGCCAATCGAGTAAGTGAGACAGCCATCCCACGCATCCGTTGATGGTCTTGCGCCCACTCCCCATCATATACGATCATTGCTGATGAAGTTTTTGGATCCCGAGCGGTTAGTTATTATAATTATTTAAATGTTTTGTCTTAATTATTATTTGTAAACAACCAACCACTAACTAAGTAATTTTTTTGCAGGCTAGCGTTAGGTCGAGTCTTGTTTCTTCTGCAGAGGAGAGTCAATACATGAATCGAGTGATGGAGCCTCCACGCGTGCCAGAGCCAGTGCATGAGCCAGTACTCGAGTCAGGGCAAGAGCCAGATCAAGAACGAGAGAGTCTACCTACTGTATCCGAGGCTAGAGTACCTGATGTAGAGGAGGCTACTGTAGTCGACACTGAGATGGTAGATGTCATTGATGCAAATAAGAGAGGAAGGGAAAAAGAGGACAAAGACAAAGGGAAAGAGAAAGAGGAGGAAGTAGAAAAAGAGGATGAGAGCAGGAAGGAGAAGAAGAAGAAGAAGAAGAAGATGAAGCAGACCTGTGAGTGTAGCCAGTGGATGGAGAGTATGGATGCTCGCATGTCTGATATGGAGACATGCCTGAAGTCCATTACCAAGTTCTTACGTAGTCTCTCTAAGGTATTTTGGTAGTCGATCATTGTTTATCGTATTGGTTCGTTTTAGTTTGTCTAACATTTGTAAACTTTCAGGGAAAATTCGTGGACCTTGAGAAGTACTTTGGACCGAAAGATGGTCCGGATGACGGGGGTGGTCCATCGAAAGGACCAGATGACATGGGTGGTCCATCAAAAGGACCGGATGACAATGAGAAGGACGGGAAGGAGAAGGACGTTGATGAGGCGTACGACATTGAGCATATTACGATAATAATATTTCTTATACTATCACATTTAAAATTTTACATTTCCATATTTAAATGTAAGTTGATATACAGGAGTTGGAGTCTCAACCAACCACTGACTTAGAGTCTCACTCAATTACTGACGTGGAGTCTCAACCAACTACAGACCCAGTCGAACTAATTGCACCTAAGGCTGAGGTACTTATTTGTCTTAATGTAAACAATATTATATAAATCTCACTAATGTATAAAATGTATGTATGTAGCCTGTTGAGTTAGGTGATGAGGAGGTTGAGGAGGTTGAAAAAGTAATCGGGCCGCATGAATTGGTAAAAAGACGAGGAAAGCGGACCCGACAAATTTCTTGGAAGCTTCGGTCTCCATGGGCTGACACCAGGCCGGACGGCAAAAGGCGAAAAGTTAAGCAATACGATCCCATGCGTGCCATTCCTGAGGAATACGAGACCAAGTTTCAGAAATGGTTGGAAGACCCATCGTCTGACGGATCGGAGCGTAAGACAGTATATGCCTACAGAAGCAAACAGTGGTTTCAGATGTTACTCAAACCGTCTCATTGGATGAGTGATGAGGTACAAACCATCTTATCACTACAGTTGTTTTTTTAATCAAGGTTTTAGGCATAGGGTTTATAGTTAAAAGTTTAGGACTTAGCGTTTATGATTTAGGGTAATTTAGGGTTTAGGACTACAATTCACAATTCTTCTGATTCTGTAGGTGATTGACTCTCTCTTCCTCTTTATTCGGAAGAAGATGGATACCCGTCTTGACTTATGTCATCGAAAGTTTGTCACAGCGGATGTATTTGTAACAGTACGTACTTTCATCTTATTGAATTTCACTTAATCTAAATTGTACTAAGATTGAATGTTGTGCAGGAATTTTTGAGGCGCGAGGATGTGTACGAAGAACTCCTTCGTAGTGACCATGGGAACGACACATTCGATTGGAGCAGATTCAAGACGGTCACTAACTACGTAATGGGAGAACACACAGATTACGTCGTTCCTTGGAGTTCCGTTGATGCTGTCTACATGCCCTTCAACTTAGGTAGAAACCATTGGGTTCTACTGTGCGCTGACTTTGAAACGGGCGAATTTGTGTTGACAGACTCCCTAACGACACTGAATTCAGATGCAGACATAGCCAAGCAGATGAATACGGTATGCACCATTTTTCCTAGGCTGCTATTAAGGTGCGACGTTATGAAGGAGAAGTCGTCTCTTCCAACACATCCATGGCGATTCAGAAGGAAGACCCAAGTGCCACAACAACAAGATAGTGGGGATTGTGGGGTTTTCACTGTAAAGTTTTTGGAATATGATGTAACTAGATCAGATTTAGGTAGTCTTAGTCAGGAGAAAATTGAGTTTTGTAGGCGTCAATTTGTTGTACAACTTTGGGCCAATAGGCCGTTCTTTTAGTAAAAAA

mRNA sequence

ATGAATCCCGGTCTCGATTTCGACCGAGATTTAGACCGGGAATTATGGCAGAGGGCTTGCATGAAGGTGGAGGAGTTAGATAAGTCGTTTCCGACCCTTCAGTTTGAGAACGACAAAGATGCAGTTAAGATCGTAGCGTTTTATTTTTTTGAGTTGGCTTTGATGGGGAGGGAACGCAAACAACAAGTAGATGCCAGCACCCTAGACTTAATGGATGATTGGGTGGCATTCTGCAATGAGGATTGGAGTACCATCGTTTTCGACAAGACGATCAAAAGTTTGAAGAAGGCACTAAAGGGGAAGGCTGAGTCGTACAAGCGTAAGGGAAGTGGTCCAAAGAAGCAGGAGACATACAGTCTATACGGTTTCCCGTTTGCTTTTCAGATATGGGCATACGAGACTGTTTCATCTCTCACTGGACGTGTTGCCAATCGAGCTAGCGTTAGGTCGAGTCTTGTTTCTTCTGCAGAGGAGAGTCAATACATGAATCGAGTGATGGAGCCTCCACGCGTGCCAGAGCCAGTGCATGAGCCAGTACTCGAGTCAGGGCAAGAGCCAGATCAAGAACGAGAGAGTCTACCTACTGTATCCGAGGCTAGAGTACCTGATGTAGAGGAGGCTACTGTAGTCGACACTGAGATGGTAGATGTCATTGATGCAAATAAGAGAGGAAGGGAAAAAGAGGACAAAGACAAAGGGAAAGAGAAAGAGGAGGAAGTAGAAAAAGAGGATGAGAGCAGGAAGGAGAAGAAGAAGAAGAAGAAGAAGATGAAGCAGACCTGTGAGTGTAGCCAGTGGATGGAGAGTATGGATGCTCGCATGTCTGATATGGAGACATGCCTGAAGTCCATTACCAAGTTCTTACGTAGTCTCTCTAAGGGAAAATTCGTGGACCTTGAGAAGTACTTTGGACCGAAAGATGGTCCGGATGACGGGGGTGGTCCATCGAAAGGACCAGATGACATGGGTGGTCCATCAAAAGGACCGGATGACAATGAGAAGGACGGGAAGGAGAAGGACGTTGATGAGGCGTACGACATTGAGCATATTACGATAATAATATTTCTTATACTATCACATTTAAAATTTTACATTTCCATATTTAAATACCCAGTCGAACTAATTGCACCTAAGGCTGAGCCTGTTGAGTTAGGTGATGAGGAGGTTGAGGAGGTTGAAAAAGTAATCGGGCCGCATGAATTGGTAAAAAGACGAGGAAAGCGGACCCGACAAATTTCTTGGAAGCTTCGGTCTCCATGGGCTGACACCAGGCCGGACGGCAAAAGGCGAAAAGTTAAGCAATACGATCCCATGCGTGCCATTCCTGAGGAATACGAGACCAAGTTTCAGAAATGGTTGGAAGACCCATCGTCTGACGGATCGGAGCCGGATGTATTTGTAACAGAATTTTTGAGGCGCGAGGATGTGTACGAAGAACTCCTTCGTAGTGACCATGGGAACGACACATTCGATTGGAGCAGATTCAAGACGGTCACTAACTACGTAATGGGAGAACACACAGATTACGTCGTTCCTTGGAGTTCCGTTGATGCTGTCTACATGCCCTTCAACTTAGGTAGAAACCATTGGGTTCTACTGTGCGCTGACTTTGAAACGGGCGAATTTGTGTTGACAGACTCCCTAACGACACTGAATTCAGATGCAGACATAGCCAAGCAGATGAATACGGTATGCACCATTTTTCCTAGGCTGCTATTAAGGTGCGACGTTATGAAGGAGAAGTCGTCTCTTCCAACACATCCATGGCGATTCAGAAGGAAGACCCAAGTGCCACAACAACAAGATAGTGGGGATTGTGGGGTTTTCACTGTAAAGTTTTTGGAATATGATGTAACTAGATCAGATTTAGGTAGTCTTAGTCAGGAGAAAATTGAGTTTTGTAGGCGTCAATTTGTTGTACAACTTTGGGCCAATAGGCCGTTCTTTTAG

Coding sequence (CDS)

ATGAATCCCGGTCTCGATTTCGACCGAGATTTAGACCGGGAATTATGGCAGAGGGCTTGCATGAAGGTGGAGGAGTTAGATAAGTCGTTTCCGACCCTTCAGTTTGAGAACGACAAAGATGCAGTTAAGATCGTAGCGTTTTATTTTTTTGAGTTGGCTTTGATGGGGAGGGAACGCAAACAACAAGTAGATGCCAGCACCCTAGACTTAATGGATGATTGGGTGGCATTCTGCAATGAGGATTGGAGTACCATCGTTTTCGACAAGACGATCAAAAGTTTGAAGAAGGCACTAAAGGGGAAGGCTGAGTCGTACAAGCGTAAGGGAAGTGGTCCAAAGAAGCAGGAGACATACAGTCTATACGGTTTCCCGTTTGCTTTTCAGATATGGGCATACGAGACTGTTTCATCTCTCACTGGACGTGTTGCCAATCGAGCTAGCGTTAGGTCGAGTCTTGTTTCTTCTGCAGAGGAGAGTCAATACATGAATCGAGTGATGGAGCCTCCACGCGTGCCAGAGCCAGTGCATGAGCCAGTACTCGAGTCAGGGCAAGAGCCAGATCAAGAACGAGAGAGTCTACCTACTGTATCCGAGGCTAGAGTACCTGATGTAGAGGAGGCTACTGTAGTCGACACTGAGATGGTAGATGTCATTGATGCAAATAAGAGAGGAAGGGAAAAAGAGGACAAAGACAAAGGGAAAGAGAAAGAGGAGGAAGTAGAAAAAGAGGATGAGAGCAGGAAGGAGAAGAAGAAGAAGAAGAAGAAGATGAAGCAGACCTGTGAGTGTAGCCAGTGGATGGAGAGTATGGATGCTCGCATGTCTGATATGGAGACATGCCTGAAGTCCATTACCAAGTTCTTACGTAGTCTCTCTAAGGGAAAATTCGTGGACCTTGAGAAGTACTTTGGACCGAAAGATGGTCCGGATGACGGGGGTGGTCCATCGAAAGGACCAGATGACATGGGTGGTCCATCAAAAGGACCGGATGACAATGAGAAGGACGGGAAGGAGAAGGACGTTGATGAGGCGTACGACATTGAGCATATTACGATAATAATATTTCTTATACTATCACATTTAAAATTTTACATTTCCATATTTAAATACCCAGTCGAACTAATTGCACCTAAGGCTGAGCCTGTTGAGTTAGGTGATGAGGAGGTTGAGGAGGTTGAAAAAGTAATCGGGCCGCATGAATTGGTAAAAAGACGAGGAAAGCGGACCCGACAAATTTCTTGGAAGCTTCGGTCTCCATGGGCTGACACCAGGCCGGACGGCAAAAGGCGAAAAGTTAAGCAATACGATCCCATGCGTGCCATTCCTGAGGAATACGAGACCAAGTTTCAGAAATGGTTGGAAGACCCATCGTCTGACGGATCGGAGCCGGATGTATTTGTAACAGAATTTTTGAGGCGCGAGGATGTGTACGAAGAACTCCTTCGTAGTGACCATGGGAACGACACATTCGATTGGAGCAGATTCAAGACGGTCACTAACTACGTAATGGGAGAACACACAGATTACGTCGTTCCTTGGAGTTCCGTTGATGCTGTCTACATGCCCTTCAACTTAGGTAGAAACCATTGGGTTCTACTGTGCGCTGACTTTGAAACGGGCGAATTTGTGTTGACAGACTCCCTAACGACACTGAATTCAGATGCAGACATAGCCAAGCAGATGAATACGGTATGCACCATTTTTCCTAGGCTGCTATTAAGGTGCGACGTTATGAAGGAGAAGTCGTCTCTTCCAACACATCCATGGCGATTCAGAAGGAAGACCCAAGTGCCACAACAACAAGATAGTGGGGATTGTGGGGTTTTCACTGTAAAGTTTTTGGAATATGATGTAACTAGATCAGATTTAGGTAGTCTTAGTCAGGAGAAAATTGAGTTTTGTAGGCGTCAATTTGTTGTACAACTTTGGGCCAATAGGCCGTTCTTTTAG

Protein sequence

MNPGLDFDRDLDRELWQRACMKVEELDKSFPTLQFENDKDAVKIVAFYFFELALMGRERKQQVDASTLDLMDDWVAFCNEDWSTIVFDKTIKSLKKALKGKAESYKRKGSGPKKQETYSLYGFPFAFQIWAYETVSSLTGRVANRASVRSSLVSSAEESQYMNRVMEPPRVPEPVHEPVLESGQEPDQERESLPTVSEARVPDVEEATVVDTEMVDVIDANKRGREKEDKDKGKEKEEEVEKEDESRKEKKKKKKKMKQTCECSQWMESMDARMSDMETCLKSITKFLRSLSKGKFVDLEKYFGPKDGPDDGGGPSKGPDDMGGPSKGPDDNEKDGKEKDVDEAYDIEHITIIIFLILSHLKFYISIFKYPVELIAPKAEPVELGDEEVEEVEKVIGPHELVKRRGKRTRQISWKLRSPWADTRPDGKRRKVKQYDPMRAIPEEYETKFQKWLEDPSSDGSEPDVFVTEFLRREDVYEELLRSDHGNDTFDWSRFKTVTNYVMGEHTDYVVPWSSVDAVYMPFNLGRNHWVLLCADFETGEFVLTDSLTTLNSDADIAKQMNTVCTIFPRLLLRCDVMKEKSSLPTHPWRFRRKTQVPQQQDSGDCGVFTVKFLEYDVTRSDLGSLSQEKIEFCRRQFVVQLWANRPFF
Homology
BLAST of Spg029067 vs. NCBI nr
Match: KAA0047553.1 (Ulp1-like peptidase [Cucumis melo var. makuwa])

HSP 1 Score: 182.2 bits (461), Expect = 1.4e-41
Identity = 183/715 (25.59%), Postives = 321/715 (44.90%), Query Frame = 0

Query: 9   RDLDRELWQRACMKVEELDKSFPTLQFE-NDKDAVKIVAFYFFELALMGRERKQQVDAST 68
           R L++    + C+ V +L+  F  L++E +D D VK+   YF E++L+G++R+ +VD   
Sbjct: 80  RLLEKFFKDKECVYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGF 139

Query: 69  LDLMDDWVAFCNEDWSTIVFDKTIKSLKKALKGKAESYKRKGSGPKKQETYSLYGFPFAF 128
             + DDW  F N DW  IVF +T+ +LK+AL  +    K+K +  KK   Y++ GF  A 
Sbjct: 140 FKIADDWNTFNNYDWGRIVFIRTLSALKRALDKQYAKGKKKSTQTKK---YTINGFLHAL 199

Query: 129 QIWAYETVSSLTG----RVANRASVRSS--LVSSAEESQYMNRVMEPPRVPEPVHEPVLE 188
           Q+WAYE++ ++ G    +V + A  R    +   + +SQ +++V + P         +++
Sbjct: 200 QVWAYESIPTIIGCGVDKVNDHAIPRMLRWVCQKSSKSQTISQVFDSPMF-------IIK 259

Query: 189 SGQEPDQERESLPTVSEARVPDVEEATVVDTEMVDVIDANKRGREKEDKDKGKEKEEEVE 248
           +  E   E E L   S     +   +T++                 + K+ G ++  EV 
Sbjct: 260 AVIEMTPEEEQLKIASGELFENFRSSTII-----------------QSKNGGSKRVREVV 319

Query: 249 KEDESRKEKKKKKKKMKQTCECSQWMESMDARMSDMETCLKSITKFLRSLSKGKFVDLEK 308
            +++  K+ KK+K K+K      + + ++  R++ +E  L SI   +  L KG    + K
Sbjct: 320 NDEDDFKKSKKQKSKIKM----KKAIRNLQDRVAVVEGQLNSIKSDIDEL-KGMMSTILK 379

Query: 309 YFGPKDGPDDGGGPSKGPDDMGGPSKGPDDNEKDGKEKDVDEAYDIEHITIIIFLILSHL 368
           + G +   D+G             S+G  D+  + KE D  +  D++      +L +   
Sbjct: 380 HIGLQRKGDEGDHK---------VSEGLVDHTLESKEVDNAKTEDVDTGGTPNWLRMPKE 439

Query: 369 KFYISIFKYPVELIAPK------AEPVE-LGDEEVEEVEKVI--GPHELVKRRGKRTRQI 428
             +I + K   E +  K       EP++ + DE+V E+E  +   PH    RR + +  +
Sbjct: 440 DEHIEVKKKGDENVLEKKVDIGLEEPIDVVDDEDVTEIEPFLTQRPHVRPARRKRASVYL 499

Query: 429 SWKLRS--PWADTRPDG-KRRKVKQYDPMRAIPEEYETKFQKWLED-------------- 488
           S    +   W+ T        +   YDPM  I + +  + + W+ D              
Sbjct: 500 STPFTTLPKWSVTSTTSTSEYEAIVYDPMHKILDVHLDRLRAWITDKRTDDELRETFHGK 559

Query: 489 -------------------------------------PSSDG--SEPDVFVTEFLRREDV 548
                                                PSS    +   +F+   + +  +
Sbjct: 560 KSKAFFRDLFMCRRWLADEHLDALFLFICLKIKAAGIPSSQNFTTADTIFMRILVSKWPL 619

Query: 549 YEELLRSDHGNDTFDWSRFKTVTNYVMGEHTDYVVPWSSVDAVYMPFNLGRNHWVLLCAD 608
           Y+E ++    N  FDW     + +YV+G   D+  PW+SVD VY PFN+  NHWVLLC D
Sbjct: 620 YKECIKE---NRPFDWDEEYRLVDYVVGSKEDFQDPWASVDYVYSPFNVHGNHWVLLCLD 679

Query: 609 FETGEFVLTDSLTTLNSDADIAKQMNTVCTIFPRLLLRCDVM--KEKSSLPTHPWRFRRK 650
             + +  + DSL +L +  ++   +  +  + P+LL        + +SS    PW     
Sbjct: 680 LVSCQVKVWDSLPSLTTAEEMTNILLPIRQLVPKLLYSTGFFDRRGRSSTYKEPWPVVIV 739

BLAST of Spg029067 vs. NCBI nr
Match: KAA0053106.1 (Ulp1-like peptidase [Cucumis melo var. makuwa] >TYK03314.1 Ulp1-like peptidase [Cucumis melo var. makuwa])

HSP 1 Score: 181.8 bits (460), Expect = 1.9e-41
Identity = 184/700 (26.29%), Postives = 316/700 (45.14%), Query Frame = 0

Query: 9   RDLDRELWQRACMKVEELDKSFPTLQFE-NDKDAVKIVAFYFFELALMGRERKQQVDAST 68
           R L++    + C+ V +L+  F  L++E +D D VK+   YF E++L+G +R+ +VD   
Sbjct: 66  RLLEKFFKDKECIYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGNDRRTKVDIGF 125

Query: 69  LDLMDDWVAFCNEDWSTIVFDKTIKSLKKALKGKAESYKRKGSGPKKQETYSLYGFPFAF 128
             + DDW  F N DW  IVF +T+ +LK+AL  +    K+K +  KK   Y++ GFP A 
Sbjct: 126 FKIADDWNTFNNYDWGRIVFGRTLSALKRALDKQYAKGKKKSTQTKK---YTINGFPHAL 185

Query: 129 QIWAYETVSSLTGRVANRAS------VRSSLVSSAEESQYMNRVMEPPRVPEPVHEPVLE 188
           Q+WAYE++S++ G   ++ +      +   +   + +SQ +++V + P +       +++
Sbjct: 186 QVWAYESISTIIGCGVDKVNDHVIPQMLRWVCQQSPKSQTISQVFDSPML-------IIK 245

Query: 189 SGQEPDQERESLPTVSEARVPDVEEATVVDTEMVDVIDANKRGREKEDKDKGKEKEEEVE 248
           +  E   E E L   S+    ++  +T+V ++       +KRGR            E V 
Sbjct: 246 AVIEMTLEEEQLKIASDELFENLRSSTIVQSKN----GGSKRGR------------EVVN 305

Query: 249 KEDESRKEKKKKKK-KMKQTCECSQWMESMDARMSDMETCLKSITKFLRSLSKGKFVDLE 308
            ED+ +K KK+K K KMK+       + ++  R++ +E  L SI   +  L KG    + 
Sbjct: 306 DEDDFKKSKKQKSKTKMKKA------IRNLQDRVAVVEGQLTSIKSNIDEL-KGMMSTIL 365

Query: 309 KYFGPKDGPDDGGGP-SKGPDDMGGPSKGPDDNEKDGKEKDVDEA---------YDIEHI 368
           K+ G +   D+G    S+G  D    SK  D    + K +DVD            + EHI
Sbjct: 366 KHIGLQRKGDEGDRKVSEGLVDHTLESKKVD----NAKTEDVDTGGTPNWLRMPKEDEHI 425

Query: 369 --------TIIIFLILSHLKFYISIFKYPVELIAPKAEPVELGDEEVEEVEKVIGPHELV 428
                    +        L+F I + K P++++  K       D +V EVE        V
Sbjct: 426 ELKKKGDEDVHTVGTPPWLRFNIELEK-PIDVLEKKV------DIDVTEVEPFPTQRTHV 485

Query: 429 KRRGKRTRQISWKLRSPWADTRPDGKRRKVKQ-----YDPMRAIPEEYETKFQKWLEDPS 488
           +   ++   +   +       R         Q     YDPM  I + +  + + W+ D  
Sbjct: 486 RPAHRKRASVYLSISFTTLPKRSVTSITTTSQSEPIIYDPMHKILDVHLDRLRAWITDKC 545

Query: 489 SDGSEPD--------------------------VFVTEFLRREDVYEELLRSDHGNDTFD 548
           +D    D                          +F+   + +  +Y+E ++    N  FD
Sbjct: 546 TDDEYLDALFLFIHLKIKAVRIPSSQNFTTADTIFMRILVVKWPLYKECIKE---NRPFD 605

Query: 549 WSRFKTVTNYVMGEHTDYVVPWSSVDAVYMPFNLGRNHWVLLCADFETGEFVLTDSLTTL 608
           W     + ++V+G   D+  PW+SVD +Y PFN+  NHWVLLC D  + +  + DS  +L
Sbjct: 606 WEEEYRLVDHVVGSKEDFQDPWASVDYIYSPFNVHTNHWVLLCLDLVSCQVKVWDSFLSL 665

Query: 609 NSDADIAKQMNTVCTIFPRLLLRCDVM--KEKSSLPTHPWRFRRKTQVPQQQDSGDCGVF 650
            +  ++   +  +  + P+LL        + +SS    PW       +P Q+++ DCGV 
Sbjct: 666 TTAEEMTNILLPIRELVPKLLDSTGFFDRRGRSSTYEEPWPVVIVDSIPLQRNNSDCGVL 716

BLAST of Spg029067 vs. NCBI nr
Match: XP_038882332.1 (uncharacterized protein LOC120073583 [Benincasa hispida])

HSP 1 Score: 180.6 bits (457), Expect = 4.2e-41
Identity = 89/172 (51.74%), Postives = 113/172 (65.70%), Query Frame = 0

Query: 478 EELLRSDHGNDTFDWSRFKTVTNYVMGEHTDYVVPWSSVDAVYMPFNLGRNHWVLLCADF 537
           E  LR D    T DWS  K V  YV G+HTDY VPWS+VDAVYMPFNL   HWVL+CADF
Sbjct: 50  ENFLRRD--RHTIDWSMEKKVLKYVHGKHTDYDVPWSNVDAVYMPFNLSGMHWVLVCADF 109

Query: 538 ETGEFVLTDSLTTLNSDADIAKQMNTVCTIFPRLLLRCDVMKEKSSLPTHPWRFRRKTQV 597
           +  E ++ DSL  L+ +AD+  +M  VC  FP LL+   VM E  +L    W  RR    
Sbjct: 110 QVRELIMFDSLIALHLNADLECEMRLVCKNFPGLLIVGAVM-ESHNLLIDRWTLRRDAFR 169

Query: 598 PQQQDSGDCGVFTVKFLEYDVTRSDLGSLSQEKIEFCRRQFVVQLWANRPFF 650
            QQ +SGDCG+FT KF EYDVT S +G+L+Q++ ++ RRQ+ +Q+WANR  F
Sbjct: 170 SQQHESGDCGMFTYKFFEYDVTGSKMGTLTQDRKQYFRRQYAIQIWANRALF 218

BLAST of Spg029067 vs. NCBI nr
Match: KAA0053486.1 (Ulp1-like peptidase [Cucumis melo var. makuwa])

HSP 1 Score: 180.3 bits (456), Expect = 5.5e-41
Identity = 187/719 (26.01%), Postives = 323/719 (44.92%), Query Frame = 0

Query: 9   RDLDRELWQRACMKVEELDKSFPTLQFE-NDKDAVKIVAFYFFELALMGRERKQQVDAST 68
           R L++    + C+ V +L+  F  L++E +D D VK+   YF E++L+G++R+ +VD   
Sbjct: 123 RLLEKFFKDKECVYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGF 182

Query: 69  LDLMDDWVAFCNEDWSTIVFDKTIKSLKKALKGKAESYKRKGSGPKKQETYSLYGFPFAF 128
             + DDW +F N DW  IVF +T+ +LK+AL  +    K+K +  KK   Y++ GFP A 
Sbjct: 183 FKIADDWNSFNNYDWGRIVFVRTLSALKRALDKQYAKGKKKSTQTKK---YTINGFPHAL 242

Query: 129 QIWAYETVSSLTG----RVANRASVRSS--LVSSAEESQYMNRVMEPPRVPEPVHEPVLE 188
           Q+WAYE++ ++ G    +V + A  R    +   + +SQ +++V + P         +++
Sbjct: 243 QVWAYESIPTIIGCGVDKVNDHAIPRMLRWVCQQSPKSQTISQVFDSPMF-------IIK 302

Query: 189 SGQEPDQERESLPTVSEARVPDVEEATVVDTEMVDVIDANKRGREKEDKDKGKEKEEEV- 248
           +  E   E E L   S     +   +T++                 + K+ G ++  EV 
Sbjct: 303 AVIEMTPEEEQLKIASGELFENFRSSTII-----------------QSKNGGSKRVREVV 362

Query: 249 -EKEDESRKEKKKKKKKMKQTCECSQWMESMDARMSDMETCLKSITKFLRSLSKGKFVDL 308
            +++D  + EK+K K KMK+         +++ R++ +E  L SI   +  L KG    +
Sbjct: 363 NDEDDFKKSEKQKSKIKMKKA------TRNLEDRVAVVEGQLNSIKSDIDEL-KGMMSTI 422

Query: 309 EKYFGPKDGPDDGGGPSKGPDDMGGPSKGPDDNEKDGKEKDVDEAYDIEHITIIIFLILS 368
            K+ G +   D+G             S+G  D+  + K+ D  +  D++      +L + 
Sbjct: 423 LKHIGLQRKGDEGDHK---------VSEGLVDHTLESKDVDNAKTEDVDTGGTPNWLRMP 482

Query: 369 HLKFYISIFKYPVELIAPK------AEPVE-LGDEEVEEVEKVI--GPHELVKRRGKRTR 428
               +I + K   E +  K       EP++ + DE+V E+E  +   PH    RR + + 
Sbjct: 483 KEDEHIEVKKKGDEDVLEKKVDIGLEEPIDVVDDEDVTEIEPFLTQRPHVRPARRKRASV 542

Query: 429 QISWKL-----RSPWADTRPDGKRRKVKQYDPMRAIPEEYETKFQKWLED---------- 488
            +S        RS  + T        V  YDPM  I + +  + + W+ D          
Sbjct: 543 YLSTPFTTLPKRSLTSTTSTSEYEAIV--YDPMHKILDVHLDRLRAWITDKRTNDELRET 602

Query: 489 -----------------------------------------PSSDG--SEPDVFVTEFLR 548
                                                    PSS    +   +F+   + 
Sbjct: 603 FHGKKSKAFFRDLFMCRRWLSDEHLDAFFLFICLKIKAAGIPSSQNFTTADTIFMRILVS 662

Query: 549 REDVYEELLRSDHGNDTFDWSRFKTVTNYVMGEHTDYVVPWSSVDAVYMPFNLGRNHWVL 608
           +  +Y+E ++    N  FDW     + +YV+G   D+  PW+SVD VY PFN+  NHWVL
Sbjct: 663 KWPLYKECIKE---NRPFDWDEEYRLVDYVVGSKEDFQDPWASVDYVYSPFNVHGNHWVL 722

Query: 609 LCADFETGEFVLTDSLTTLNSDADIAKQMNTVCTIFPRLLLRCDVM--KEKSSLPTHPWR 650
           LC D  + +  + DSL +L +  D+   +  +  + P+LL        + +SS    PW 
Sbjct: 723 LCLDLVSCQVKVWDSLPSLTTAEDMTNILLPIRQLVPKLLDSTGFFDRRGRSSTYKEPWP 782

BLAST of Spg029067 vs. NCBI nr
Match: KAA0062032.1 (Ulp1-like peptidase [Cucumis melo var. makuwa])

HSP 1 Score: 179.9 bits (455), Expect = 7.2e-41
Identity = 188/691 (27.21%), Postives = 323/691 (46.74%), Query Frame = 0

Query: 9   RDLDRELWQRACMKVEELDKSFPTLQFE-NDKDAVKIVAFYFFELALMGRERKQQVDAST 68
           R L++    + C+ V +L+  F  L++E +D D VK+   YF E++L+G++R+ +VD   
Sbjct: 43  RLLEKFFKDKECIYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGF 102

Query: 69  LDLMDDWVAFCNEDWSTIVFDKTIKSLKKALKGKAESYKRKGSGPKKQETYSLYGFPFAF 128
             + DDW  F N DW  IVF +T+ +LK+AL  +    K+K    KK   Y++ GF  A 
Sbjct: 103 FKIADDWNTFNNYDWDQIVFGRTLSALKRALDKQYAKGKKKSIQTKK---YTINGFSHAL 162

Query: 129 QIWAYETVSSLTG----RVANRASVRSS--LVSSAEESQYMNRVMEPPRVPEPVHEPVLE 188
           Q+WAYE++ ++ G    +V + A  R    +   + +SQ +++V + P +       +++
Sbjct: 163 QVWAYESIPTIIGCGIDKVNDHAIPRMLRWVCQQSPKSQTISQVFDSPML-------IIK 222

Query: 189 SGQEPDQERESLPTVSEARVPDVEEATVVDTEMVDVIDANKRGREKEDKDKGKEKEEEVE 248
           +  E   E E L   S     ++  +T+V ++       +KRGR            E V 
Sbjct: 223 AVIEMTPEEEQLKITSGELFENLHSSTIVQSKN----GGSKRGR------------EVVN 282

Query: 249 KEDESRKEKKKKKK-KMKQTCECSQWMESMDARMSDMETCLKSITKFLRSLSKGKFVDLE 308
            ED+ +K KK+K K KMK+       + ++  R++ +E  L SI   +  L KG    + 
Sbjct: 283 DEDDFKKSKKQKSKTKMKKA------IRNLQDRVAVVEGQLTSINSDIDEL-KGMMSTIL 342

Query: 309 KYFGPKDGPDDGGGP---------SKGPDDMGGPSKGPDDNEKDGKEKDVDEA------- 368
           K+ G +    +                P+ +  P +      K   +KDV          
Sbjct: 343 KHIGLQRKVRNETVKCIDVSCFIFGVTPNWLRMPKEDEHIELKKKGDKDVHTVGTPPWLR 402

Query: 369 YDIE---HITIIIFLILSHLKFYISIFKYPVELIAPKAEPVELGDEEVEEVEKVIGPHEL 428
           ++IE    I ++   +   L+  I +    VE+   + +     DE+V E+E    P + 
Sbjct: 403 FNIELEKSIDVLEKKVDIGLEEPIDVVDDEVEIEDSRKKLRTHFDEDVIEIEPF--PTQR 462

Query: 429 VKRRGKRTRQISWKLRSPWADTRPDGKRRKVKQ-----------YDPMRAIPEEYETKFQ 488
              R  R ++ S  L +P+  T P   +R V             YDPM  I + +  + +
Sbjct: 463 PHVRPTRRKRASIYLLTPFT-TLP---KRSVTSTTTTSQSEPIVYDPMYKILDVHLDRLR 522

Query: 489 KWLED--------PSSDG--SEPDVFVTEFLRREDVYEELLRSDHGNDTFDWSRFKTVTN 548
            W+          PSS    +   +F+   + +  +Y+E ++ +H    FDW     + +
Sbjct: 523 AWITTSVQTRAGIPSSQNFTTADTIFMRILVAKWHLYKECIKENH---PFDWEEEYRLVD 582

Query: 549 YVMGEHTDYVVPWSSVDAVYMPFNLGRNHWVLLCADFETGEFVLTDSLTTLNSDADIAKQ 608
           YV+G   D+  PW+SVD VY PFN+  NHWVLLC D  + +  + DSL +L +  ++   
Sbjct: 583 YVVGSKEDFQDPWASVDYVYSPFNVHANHWVLLCLDLVSCQVKIWDSLPSLTTAEEMTNI 642

Query: 609 MNTVCTIFPRLLLRCDVM--KEKSSLPTHPWRFRRKTQVPQQQDSGDCGVFTVKFLEYDV 650
           + ++  + P+LL        + +SS    PW       +P Q+++ DCGVFT+K+ EY  
Sbjct: 643 LLSIRELVPKLLDSTGFFDRRGRSSTYKEPWPVVIVDSIPLQRNNSDCGVFTIKYFEYIA 689

BLAST of Spg029067 vs. ExPASy Swiss-Prot
Match: Q09353 (Sentrin-specific protease OS=Caenorhabditis elegans OX=6239 GN=ulp-1 PE=2 SV=3)

HSP 1 Score: 48.5 bits (114), Expect = 3.3e-04
Identity = 32/126 (25.40%), Postives = 59/126 (46.83%), Query Frame = 0

Query: 517 DAVYMPFNLGRNHWVLLCADFETGEFVLTDSLTTLNSDADIAKQMNTVCTIFPRLLLRCD 576
           D V +P +LG  HW +   D    +    DSL   N+          V       L    
Sbjct: 574 DIVLVPVHLGM-HWCMAVIDMGEKKIEFYDSLYDGNT---------AVLPALRGYLEAES 633

Query: 577 VMKEKSSLPTHPWRFRRKTQVPQQQDSGDCGVFTVKFLEYDVTRSDLGSLSQEKIEFCRR 636
           + K+K+++    W  ++ T +P+QQ+  DCGVF+ +F E+  +R      +Q+ + + R+
Sbjct: 634 LDKKKTAMNFSGWTIQQMTDIPRQQNGSDCGVFSCQFGEW-ASRRTTPRFTQKNMPYYRK 688

Query: 637 QFVVQL 643
           + V ++
Sbjct: 694 RMVYEI 688

BLAST of Spg029067 vs. ExPASy TrEMBL
Match: A0A5A7TVI1 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold115G00120 PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 7.0e-42
Identity = 183/715 (25.59%), Postives = 321/715 (44.90%), Query Frame = 0

Query: 9   RDLDRELWQRACMKVEELDKSFPTLQFE-NDKDAVKIVAFYFFELALMGRERKQQVDAST 68
           R L++    + C+ V +L+  F  L++E +D D VK+   YF E++L+G++R+ +VD   
Sbjct: 80  RLLEKFFKDKECVYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGF 139

Query: 69  LDLMDDWVAFCNEDWSTIVFDKTIKSLKKALKGKAESYKRKGSGPKKQETYSLYGFPFAF 128
             + DDW  F N DW  IVF +T+ +LK+AL  +    K+K +  KK   Y++ GF  A 
Sbjct: 140 FKIADDWNTFNNYDWGRIVFIRTLSALKRALDKQYAKGKKKSTQTKK---YTINGFLHAL 199

Query: 129 QIWAYETVSSLTG----RVANRASVRSS--LVSSAEESQYMNRVMEPPRVPEPVHEPVLE 188
           Q+WAYE++ ++ G    +V + A  R    +   + +SQ +++V + P         +++
Sbjct: 200 QVWAYESIPTIIGCGVDKVNDHAIPRMLRWVCQKSSKSQTISQVFDSPMF-------IIK 259

Query: 189 SGQEPDQERESLPTVSEARVPDVEEATVVDTEMVDVIDANKRGREKEDKDKGKEKEEEVE 248
           +  E   E E L   S     +   +T++                 + K+ G ++  EV 
Sbjct: 260 AVIEMTPEEEQLKIASGELFENFRSSTII-----------------QSKNGGSKRVREVV 319

Query: 249 KEDESRKEKKKKKKKMKQTCECSQWMESMDARMSDMETCLKSITKFLRSLSKGKFVDLEK 308
            +++  K+ KK+K K+K      + + ++  R++ +E  L SI   +  L KG    + K
Sbjct: 320 NDEDDFKKSKKQKSKIKM----KKAIRNLQDRVAVVEGQLNSIKSDIDEL-KGMMSTILK 379

Query: 309 YFGPKDGPDDGGGPSKGPDDMGGPSKGPDDNEKDGKEKDVDEAYDIEHITIIIFLILSHL 368
           + G +   D+G             S+G  D+  + KE D  +  D++      +L +   
Sbjct: 380 HIGLQRKGDEGDHK---------VSEGLVDHTLESKEVDNAKTEDVDTGGTPNWLRMPKE 439

Query: 369 KFYISIFKYPVELIAPK------AEPVE-LGDEEVEEVEKVI--GPHELVKRRGKRTRQI 428
             +I + K   E +  K       EP++ + DE+V E+E  +   PH    RR + +  +
Sbjct: 440 DEHIEVKKKGDENVLEKKVDIGLEEPIDVVDDEDVTEIEPFLTQRPHVRPARRKRASVYL 499

Query: 429 SWKLRS--PWADTRPDG-KRRKVKQYDPMRAIPEEYETKFQKWLED-------------- 488
           S    +   W+ T        +   YDPM  I + +  + + W+ D              
Sbjct: 500 STPFTTLPKWSVTSTTSTSEYEAIVYDPMHKILDVHLDRLRAWITDKRTDDELRETFHGK 559

Query: 489 -------------------------------------PSSDG--SEPDVFVTEFLRREDV 548
                                                PSS    +   +F+   + +  +
Sbjct: 560 KSKAFFRDLFMCRRWLADEHLDALFLFICLKIKAAGIPSSQNFTTADTIFMRILVSKWPL 619

Query: 549 YEELLRSDHGNDTFDWSRFKTVTNYVMGEHTDYVVPWSSVDAVYMPFNLGRNHWVLLCAD 608
           Y+E ++    N  FDW     + +YV+G   D+  PW+SVD VY PFN+  NHWVLLC D
Sbjct: 620 YKECIKE---NRPFDWDEEYRLVDYVVGSKEDFQDPWASVDYVYSPFNVHGNHWVLLCLD 679

Query: 609 FETGEFVLTDSLTTLNSDADIAKQMNTVCTIFPRLLLRCDVM--KEKSSLPTHPWRFRRK 650
             + +  + DSL +L +  ++   +  +  + P+LL        + +SS    PW     
Sbjct: 680 LVSCQVKVWDSLPSLTTAEEMTNILLPIRQLVPKLLYSTGFFDRRGRSSTYKEPWPVVIV 739

BLAST of Spg029067 vs. ExPASy TrEMBL
Match: A0A5A7UFV4 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold566G00020 PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 9.2e-42
Identity = 184/700 (26.29%), Postives = 316/700 (45.14%), Query Frame = 0

Query: 9   RDLDRELWQRACMKVEELDKSFPTLQFE-NDKDAVKIVAFYFFELALMGRERKQQVDAST 68
           R L++    + C+ V +L+  F  L++E +D D VK+   YF E++L+G +R+ +VD   
Sbjct: 66  RLLEKFFKDKECIYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGNDRRTKVDIGF 125

Query: 69  LDLMDDWVAFCNEDWSTIVFDKTIKSLKKALKGKAESYKRKGSGPKKQETYSLYGFPFAF 128
             + DDW  F N DW  IVF +T+ +LK+AL  +    K+K +  KK   Y++ GFP A 
Sbjct: 126 FKIADDWNTFNNYDWGRIVFGRTLSALKRALDKQYAKGKKKSTQTKK---YTINGFPHAL 185

Query: 129 QIWAYETVSSLTGRVANRAS------VRSSLVSSAEESQYMNRVMEPPRVPEPVHEPVLE 188
           Q+WAYE++S++ G   ++ +      +   +   + +SQ +++V + P +       +++
Sbjct: 186 QVWAYESISTIIGCGVDKVNDHVIPQMLRWVCQQSPKSQTISQVFDSPML-------IIK 245

Query: 189 SGQEPDQERESLPTVSEARVPDVEEATVVDTEMVDVIDANKRGREKEDKDKGKEKEEEVE 248
           +  E   E E L   S+    ++  +T+V ++       +KRGR            E V 
Sbjct: 246 AVIEMTLEEEQLKIASDELFENLRSSTIVQSKN----GGSKRGR------------EVVN 305

Query: 249 KEDESRKEKKKKKK-KMKQTCECSQWMESMDARMSDMETCLKSITKFLRSLSKGKFVDLE 308
            ED+ +K KK+K K KMK+       + ++  R++ +E  L SI   +  L KG    + 
Sbjct: 306 DEDDFKKSKKQKSKTKMKKA------IRNLQDRVAVVEGQLTSIKSNIDEL-KGMMSTIL 365

Query: 309 KYFGPKDGPDDGGGP-SKGPDDMGGPSKGPDDNEKDGKEKDVDEA---------YDIEHI 368
           K+ G +   D+G    S+G  D    SK  D    + K +DVD            + EHI
Sbjct: 366 KHIGLQRKGDEGDRKVSEGLVDHTLESKKVD----NAKTEDVDTGGTPNWLRMPKEDEHI 425

Query: 369 --------TIIIFLILSHLKFYISIFKYPVELIAPKAEPVELGDEEVEEVEKVIGPHELV 428
                    +        L+F I + K P++++  K       D +V EVE        V
Sbjct: 426 ELKKKGDEDVHTVGTPPWLRFNIELEK-PIDVLEKKV------DIDVTEVEPFPTQRTHV 485

Query: 429 KRRGKRTRQISWKLRSPWADTRPDGKRRKVKQ-----YDPMRAIPEEYETKFQKWLEDPS 488
           +   ++   +   +       R         Q     YDPM  I + +  + + W+ D  
Sbjct: 486 RPAHRKRASVYLSISFTTLPKRSVTSITTTSQSEPIIYDPMHKILDVHLDRLRAWITDKC 545

Query: 489 SDGSEPD--------------------------VFVTEFLRREDVYEELLRSDHGNDTFD 548
           +D    D                          +F+   + +  +Y+E ++    N  FD
Sbjct: 546 TDDEYLDALFLFIHLKIKAVRIPSSQNFTTADTIFMRILVVKWPLYKECIKE---NRPFD 605

Query: 549 WSRFKTVTNYVMGEHTDYVVPWSSVDAVYMPFNLGRNHWVLLCADFETGEFVLTDSLTTL 608
           W     + ++V+G   D+  PW+SVD +Y PFN+  NHWVLLC D  + +  + DS  +L
Sbjct: 606 WEEEYRLVDHVVGSKEDFQDPWASVDYIYSPFNVHTNHWVLLCLDLVSCQVKVWDSFLSL 665

Query: 609 NSDADIAKQMNTVCTIFPRLLLRCDVM--KEKSSLPTHPWRFRRKTQVPQQQDSGDCGVF 650
            +  ++   +  +  + P+LL        + +SS    PW       +P Q+++ DCGV 
Sbjct: 666 TTAEEMTNILLPIRELVPKLLDSTGFFDRRGRSSTYEEPWPVVIVDSIPLQRNNSDCGVL 716

BLAST of Spg029067 vs. ExPASy TrEMBL
Match: A0A5A7UGY3 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold190G00350 PE=3 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 2.7e-41
Identity = 187/719 (26.01%), Postives = 323/719 (44.92%), Query Frame = 0

Query: 9   RDLDRELWQRACMKVEELDKSFPTLQFE-NDKDAVKIVAFYFFELALMGRERKQQVDAST 68
           R L++    + C+ V +L+  F  L++E +D D VK+   YF E++L+G++R+ +VD   
Sbjct: 123 RLLEKFFKDKECVYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGF 182

Query: 69  LDLMDDWVAFCNEDWSTIVFDKTIKSLKKALKGKAESYKRKGSGPKKQETYSLYGFPFAF 128
             + DDW +F N DW  IVF +T+ +LK+AL  +    K+K +  KK   Y++ GFP A 
Sbjct: 183 FKIADDWNSFNNYDWGRIVFVRTLSALKRALDKQYAKGKKKSTQTKK---YTINGFPHAL 242

Query: 129 QIWAYETVSSLTG----RVANRASVRSS--LVSSAEESQYMNRVMEPPRVPEPVHEPVLE 188
           Q+WAYE++ ++ G    +V + A  R    +   + +SQ +++V + P         +++
Sbjct: 243 QVWAYESIPTIIGCGVDKVNDHAIPRMLRWVCQQSPKSQTISQVFDSPMF-------IIK 302

Query: 189 SGQEPDQERESLPTVSEARVPDVEEATVVDTEMVDVIDANKRGREKEDKDKGKEKEEEV- 248
           +  E   E E L   S     +   +T++                 + K+ G ++  EV 
Sbjct: 303 AVIEMTPEEEQLKIASGELFENFRSSTII-----------------QSKNGGSKRVREVV 362

Query: 249 -EKEDESRKEKKKKKKKMKQTCECSQWMESMDARMSDMETCLKSITKFLRSLSKGKFVDL 308
            +++D  + EK+K K KMK+         +++ R++ +E  L SI   +  L KG    +
Sbjct: 363 NDEDDFKKSEKQKSKIKMKKA------TRNLEDRVAVVEGQLNSIKSDIDEL-KGMMSTI 422

Query: 309 EKYFGPKDGPDDGGGPSKGPDDMGGPSKGPDDNEKDGKEKDVDEAYDIEHITIIIFLILS 368
            K+ G +   D+G             S+G  D+  + K+ D  +  D++      +L + 
Sbjct: 423 LKHIGLQRKGDEGDHK---------VSEGLVDHTLESKDVDNAKTEDVDTGGTPNWLRMP 482

Query: 369 HLKFYISIFKYPVELIAPK------AEPVE-LGDEEVEEVEKVI--GPHELVKRRGKRTR 428
               +I + K   E +  K       EP++ + DE+V E+E  +   PH    RR + + 
Sbjct: 483 KEDEHIEVKKKGDEDVLEKKVDIGLEEPIDVVDDEDVTEIEPFLTQRPHVRPARRKRASV 542

Query: 429 QISWKL-----RSPWADTRPDGKRRKVKQYDPMRAIPEEYETKFQKWLED---------- 488
            +S        RS  + T        V  YDPM  I + +  + + W+ D          
Sbjct: 543 YLSTPFTTLPKRSLTSTTSTSEYEAIV--YDPMHKILDVHLDRLRAWITDKRTNDELRET 602

Query: 489 -----------------------------------------PSSDG--SEPDVFVTEFLR 548
                                                    PSS    +   +F+   + 
Sbjct: 603 FHGKKSKAFFRDLFMCRRWLSDEHLDAFFLFICLKIKAAGIPSSQNFTTADTIFMRILVS 662

Query: 549 REDVYEELLRSDHGNDTFDWSRFKTVTNYVMGEHTDYVVPWSSVDAVYMPFNLGRNHWVL 608
           +  +Y+E ++    N  FDW     + +YV+G   D+  PW+SVD VY PFN+  NHWVL
Sbjct: 663 KWPLYKECIKE---NRPFDWDEEYRLVDYVVGSKEDFQDPWASVDYVYSPFNVHGNHWVL 722

Query: 609 LCADFETGEFVLTDSLTTLNSDADIAKQMNTVCTIFPRLLLRCDVM--KEKSSLPTHPWR 650
           LC D  + +  + DSL +L +  D+   +  +  + P+LL        + +SS    PW 
Sbjct: 723 LCLDLVSCQVKVWDSLPSLTTAEDMTNILLPIRQLVPKLLDSTGFFDRRGRSSTYKEPWP 782

BLAST of Spg029067 vs. ExPASy TrEMBL
Match: A0A5A7V1E3 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold89G003680 PE=3 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 3.5e-41
Identity = 188/691 (27.21%), Postives = 323/691 (46.74%), Query Frame = 0

Query: 9   RDLDRELWQRACMKVEELDKSFPTLQFE-NDKDAVKIVAFYFFELALMGRERKQQVDAST 68
           R L++    + C+ V +L+  F  L++E +D D VK+   YF E++L+G++R+ +VD   
Sbjct: 43  RLLEKFFKDKECIYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGF 102

Query: 69  LDLMDDWVAFCNEDWSTIVFDKTIKSLKKALKGKAESYKRKGSGPKKQETYSLYGFPFAF 128
             + DDW  F N DW  IVF +T+ +LK+AL  +    K+K    KK   Y++ GF  A 
Sbjct: 103 FKIADDWNTFNNYDWDQIVFGRTLSALKRALDKQYAKGKKKSIQTKK---YTINGFSHAL 162

Query: 129 QIWAYETVSSLTG----RVANRASVRSS--LVSSAEESQYMNRVMEPPRVPEPVHEPVLE 188
           Q+WAYE++ ++ G    +V + A  R    +   + +SQ +++V + P +       +++
Sbjct: 163 QVWAYESIPTIIGCGIDKVNDHAIPRMLRWVCQQSPKSQTISQVFDSPML-------IIK 222

Query: 189 SGQEPDQERESLPTVSEARVPDVEEATVVDTEMVDVIDANKRGREKEDKDKGKEKEEEVE 248
           +  E   E E L   S     ++  +T+V ++       +KRGR            E V 
Sbjct: 223 AVIEMTPEEEQLKITSGELFENLHSSTIVQSKN----GGSKRGR------------EVVN 282

Query: 249 KEDESRKEKKKKKK-KMKQTCECSQWMESMDARMSDMETCLKSITKFLRSLSKGKFVDLE 308
            ED+ +K KK+K K KMK+       + ++  R++ +E  L SI   +  L KG    + 
Sbjct: 283 DEDDFKKSKKQKSKTKMKKA------IRNLQDRVAVVEGQLTSINSDIDEL-KGMMSTIL 342

Query: 309 KYFGPKDGPDDGGGP---------SKGPDDMGGPSKGPDDNEKDGKEKDVDEA------- 368
           K+ G +    +                P+ +  P +      K   +KDV          
Sbjct: 343 KHIGLQRKVRNETVKCIDVSCFIFGVTPNWLRMPKEDEHIELKKKGDKDVHTVGTPPWLR 402

Query: 369 YDIE---HITIIIFLILSHLKFYISIFKYPVELIAPKAEPVELGDEEVEEVEKVIGPHEL 428
           ++IE    I ++   +   L+  I +    VE+   + +     DE+V E+E    P + 
Sbjct: 403 FNIELEKSIDVLEKKVDIGLEEPIDVVDDEVEIEDSRKKLRTHFDEDVIEIEPF--PTQR 462

Query: 429 VKRRGKRTRQISWKLRSPWADTRPDGKRRKVKQ-----------YDPMRAIPEEYETKFQ 488
              R  R ++ S  L +P+  T P   +R V             YDPM  I + +  + +
Sbjct: 463 PHVRPTRRKRASIYLLTPFT-TLP---KRSVTSTTTTSQSEPIVYDPMYKILDVHLDRLR 522

Query: 489 KWLED--------PSSDG--SEPDVFVTEFLRREDVYEELLRSDHGNDTFDWSRFKTVTN 548
            W+          PSS    +   +F+   + +  +Y+E ++ +H    FDW     + +
Sbjct: 523 AWITTSVQTRAGIPSSQNFTTADTIFMRILVAKWHLYKECIKENH---PFDWEEEYRLVD 582

Query: 549 YVMGEHTDYVVPWSSVDAVYMPFNLGRNHWVLLCADFETGEFVLTDSLTTLNSDADIAKQ 608
           YV+G   D+  PW+SVD VY PFN+  NHWVLLC D  + +  + DSL +L +  ++   
Sbjct: 583 YVVGSKEDFQDPWASVDYVYSPFNVHANHWVLLCLDLVSCQVKIWDSLPSLTTAEEMTNI 642

Query: 609 MNTVCTIFPRLLLRCDVM--KEKSSLPTHPWRFRRKTQVPQQQDSGDCGVFTVKFLEYDV 650
           + ++  + P+LL        + +SS    PW       +P Q+++ DCGVFT+K+ EY  
Sbjct: 643 LLSIRELVPKLLDSTGFFDRRGRSSTYKEPWPVVIVDSIPLQRNNSDCGVFTIKYFEYIA 689

BLAST of Spg029067 vs. ExPASy TrEMBL
Match: A0A5D3C2G7 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00140 PE=3 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 5.9e-41
Identity = 179/680 (26.32%), Postives = 311/680 (45.74%), Query Frame = 0

Query: 9   RDLDRELWQRACMKVEELDKSFPTLQFE-NDKDAVKIVAFYFFELALMGRERKQQVDAST 68
           R L++    + C+ V +L+  F  L++E +D D VK+   YF E++L+G++R+ +VD   
Sbjct: 110 RLLEKFFKDKDCVYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGF 169

Query: 69  LDLMDDWVAFCNEDWSTIVFDKTIKSLKKALKGKAESYKRKGSGPKKQETYSLYGFPFAF 128
             + DDW +F N DW  IVF +T+ +LK+AL  +    K+K +  KK   Y++ GFP A 
Sbjct: 170 FKIADDWNSFNNYDWGRIVFVRTLSALKRALDKQYAKGKKKSTQTKK---YTINGFPHAL 229

Query: 129 QIWAYETVSSLTG----RVANRASVRSS--LVSSAEESQYMNRVMEPPRVPEPVHEPVLE 188
           Q+WAYE++ ++ G    +V + A  R    +   + +SQ +++V + P         +++
Sbjct: 230 QVWAYESIPTIIGCGVDKVNDHAIPRMLRWVCQQSPKSQTISQVFDSPMF-------IIK 289

Query: 189 SGQEPDQERESLPTVSEARVPDVEEATVVDTEMVDVIDANKRGREKEDKDKGKEKEEEVE 248
           +  E   E E L   S     +   +T++                 + K+ G ++  EV 
Sbjct: 290 AVIEMTPEEEQLKIASGELFENFRSSTII-----------------QSKNGGSKRVREVV 349

Query: 249 KEDESRKEKKKKKKKMKQTCECSQWMESMDARMSDMETCLKSITKFLRSLSKGKFVDLEK 308
            +++  K+ KK+K K+K      + + ++  R++ +E  L +I   +  L KG    + K
Sbjct: 350 NDEDELKKSKKQKSKIKM----KKAIRNLQDRVAVVEGQLNTIKSDIDEL-KGLMSTILK 409

Query: 309 YFGPKDGPDDGGGP-SKGPDDMGGPSKGPDDNEKDGKEKDVDEAYDIEHITIIIFLILSH 368
           + G +   D+G    S+G +D     K   D  +D  EK VD   +              
Sbjct: 410 HIGLQRKGDEGDHKVSEGLEDEHIEVKKKGD--EDVLEKKVDIGLE-------------- 469

Query: 369 LKFYISIFKYPVELIAPKAEPVE-LGDEEVEEVEKVI--GPHELVKRRGKRTRQISWKL- 428
                              EP++ + DE+V E+E  +   PH    RR + +  +S    
Sbjct: 470 -------------------EPIDVVDDEDVIEIEPFLTQRPHVRPARRKRASVYLSTPFT 529

Query: 429 ----RSPWADTRPDGKRRKVKQYDPMRAIPEEYETKFQKWLEDPSSD--------GSEPD 488
               RS  + T        V  YDPM  I + +  + + W+ D  +D        G +  
Sbjct: 530 TLPKRSLTSTTSTSESEAIV--YDPMHKILDVHLDRLRAWITDKRTDDELRETFHGKKSK 589

Query: 489 VFVTE-------------FLRREDVYEELLRSDHGNDTFDWSRFKTVTNYVMGEHTDYVV 548
            F  +              + +  +Y+E ++    N  FDW     + +YV+G   D+  
Sbjct: 590 AFFRDLFMCRRWLSDERILVSKWPLYKECIKE---NRPFDWDEEYRLVDYVVGSKVDFQD 649

Query: 549 PWSSVDAVYMPFNLGRNHWVLLCADFETGEFVLTDSLTTLNSDADIAKQMNTVCTIFPRL 608
           PW+SVD VY PFN+  NHWVLLC D  + +  + DSL +L +  ++   +  +  + P+L
Sbjct: 650 PWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDSLPSLTTAEEMTNILLPIRQLVPKL 709

Query: 609 LLRCDVM--KEKSSLPTHPWRFRRKTQVPQQQDSGDCGVFTVKFLEYDVTRSDLGSLSQE 650
           L        + +SS    PW       +P Q+++ DCGVF +K+ EY      L +L QE
Sbjct: 710 LDSTGFFDRRGRSSTYKEPWPVVIVDPIPLQRNNCDCGVFAIKYFEYIAAGVGLDTLCQE 715

BLAST of Spg029067 vs. TAIR 10
Match: AT5G45570.1 (Ulp1 protease family protein )

HSP 1 Score: 62.4 bits (150), Expect = 1.6e-09
Identity = 35/129 (27.13%), Postives = 63/129 (48.84%), Query Frame = 0

Query: 516 VDAVYMPFNLGRNHWVLLCADFETGEFVLTDSLTTLNSDADIAKQMNTVCTIFPRLLLRC 575
           VD +Y    +  NHWV L  D       + DS+ +L +D ++A Q   V T+ P +L   
Sbjct: 768 VDHLYAYLFVNGNHWVALDIDLTNKRVNVYDSIPSLTTDTEMAIQCMFVMTMIPAMLSSF 827

Query: 576 DVMKE-KSSLPTHPWRFRRKTQVPQQQDSGDCGVFTVKFLEYDVTRSDLGSLSQEKIEFC 635
              K+ + S     W  +R T++P+  D GDC ++++K++E          L  E ++  
Sbjct: 828 IPSKQRRRSYSKLEW--KRITKIPENLDPGDCAIYSIKYIECLALGKSFDGLCDENMQSL 887

Query: 636 RRQFVVQLW 644
           R +  V+++
Sbjct: 888 RTKLAVEMF 894

BLAST of Spg029067 vs. TAIR 10
Match: AT2G07240.1 (cysteine-type peptidases;cysteine-type peptidases )

HSP 1 Score: 56.2 bits (134), Expect = 1.1e-07
Identity = 28/99 (28.28%), Postives = 44/99 (44.44%), Query Frame = 0

Query: 513 WSSVDAVYMPFNLGRNHWVLLCADFETGEFVLTDSLTTLNSDADIAKQMNTVCTIFPRLL 572
           ++  D VYMPFN  + HWV LC D +  +  + DS   L  DA +  ++  +  + P L 
Sbjct: 738 FAEADCVYMPFNFDKKHWVSLCVDLKAHKITILDSNIQLRRDAALYAELQPLAAMLPYLF 797

Query: 573 LRCDVMKEKSSLPTHPWRFRRKTQVPQQQDSGDCGVFTV 612
            +         +   P+   R   +PQ     D GV +V
Sbjct: 798 RQ--TSSSSGDISLQPFLLDRPHGIPQVSSPFDSGVLSV 834

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0047553.11.4e-4125.59Ulp1-like peptidase [Cucumis melo var. makuwa][more]
KAA0053106.11.9e-4126.29Ulp1-like peptidase [Cucumis melo var. makuwa] >TYK03314.1 Ulp1-like peptidase [... [more]
XP_038882332.14.2e-4151.74uncharacterized protein LOC120073583 [Benincasa hispida][more]
KAA0053486.15.5e-4126.01Ulp1-like peptidase [Cucumis melo var. makuwa][more]
KAA0062032.17.2e-4127.21Ulp1-like peptidase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q093533.3e-0425.40Sentrin-specific protease OS=Caenorhabditis elegans OX=6239 GN=ulp-1 PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A5A7TVI17.0e-4225.59Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold115G... [more]
A0A5A7UFV49.2e-4226.29Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold566G... [more]
A0A5A7UGY32.7e-4126.01Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold190G... [more]
A0A5A7V1E33.5e-4127.21Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold89G0... [more]
A0A5D3C2G75.9e-4126.32Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G0... [more]
Match NameE-valueIdentityDescription
AT5G45570.11.6e-0927.13Ulp1 protease family protein [more]
AT2G07240.11.1e-0728.28cysteine-type peptidases;cysteine-type peptidases [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 233..260
NoneNo IPR availableGENE3D3.40.395.10Adenoviral Proteinase; Chain Acoord: 475..646
e-value: 2.6E-24
score: 87.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 216..250
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 324..340
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 302..340
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 180..196
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 153..262
NoneNo IPR availablePANTHERPTHR12606:SF109BNACNNG52470D PROTEINcoord: 150..643
NoneNo IPR availablePANTHERPTHR12606SENTRIN/SUMO-SPECIFIC PROTEASEcoord: 150..643
IPR003653Ulp1 protease family, C-terminal catalytic domainPFAMPF02902Peptidase_C48coord: 515..638
e-value: 6.2E-20
score: 72.0
IPR003653Ulp1 protease family, C-terminal catalytic domainPROSITEPS50600ULP_PROTEASEcoord: 430..617
score: 13.967588
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 488..644

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg029067.1Spg029067.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008234 cysteine-type peptidase activity