Spg029018 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg029018
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Sigma factor binding protein 2
Location	scaffold5: 15396644 .. 15397057 (-)
RNA-Seq Expression	Spg029018
Synteny	Spg029018

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCCAAGTTTGATTGCTGGCGACGTGCAAGAGACGACGAAGAAGAAAGCAACGAGGAAAAGAAAATCCTCTGACCCGAAGAACCAACCCCTCAAAGTTGTCTACATATCCAACCCCATGAGGGTTCAAACCAGCGCCGCTGAATTCCGAGCCTTGGTCCAAGAACTCACCGGCCGAGACGCCGATTTCCCAGACCCGACCAAGTTTCCATCGCCCTCGGTCCATGCTCTGGAGACAGATCCTCCGAAGAAACCAGAAGAAGAAGACGACGACAACGACAACGACGACGAACTACTGTTACTTGATTTGGCTGCAATGGAGGATTCCAGCAGCGATGATTTTCTTGAGGGTTCGTATGGGACTTTGGAGGATATTTTCACGAGGGATATGGTGGAGAAATTGGCCGCCTGA

mRNA sequence

ATGGATCCAAGTTTGATTGCTGGCGACGTGCAAGAGACGACGAAGAAGAAAGCAACGAGGAAAAGAAAATCCTCTGACCCGAAGAACCAACCCCTCAAAGTTGTCTACATATCCAACCCCATGAGGGTTCAAACCAGCGCCGCTGAATTCCGAGCCTTGGTCCAAGAACTCACCGGCCGAGACGCCGATTTCCCAGACCCGACCAAGTTTCCATCGCCCTCGGTCCATGCTCTGGAGACAGATCCTCCGAAGAAACCAGAAGAAGAAGACGACGACAACGACAACGACGACGAACTACTGTTACTTGATTTGGCTGCAATGGAGGATTCCAGCAGCGATGATTTTCTTGAGGGTTCGTATGGGACTTTGGAGGATATTTTCACGAGGGATATGGTGGAGAAATTGGCCGCCTGA

Coding sequence (CDS)

ATGGATCCAAGTTTGATTGCTGGCGACGTGCAAGAGACGACGAAGAAGAAAGCAACGAGGAAAAGAAAATCCTCTGACCCGAAGAACCAACCCCTCAAAGTTGTCTACATATCCAACCCCATGAGGGTTCAAACCAGCGCCGCTGAATTCCGAGCCTTGGTCCAAGAACTCACCGGCCGAGACGCCGATTTCCCAGACCCGACCAAGTTTCCATCGCCCTCGGTCCATGCTCTGGAGACAGATCCTCCGAAGAAACCAGAAGAAGAAGACGACGACAACGACAACGACGACGAACTACTGTTACTTGATTTGGCTGCAATGGAGGATTCCAGCAGCGATGATTTTCTTGAGGGTTCGTATGGGACTTTGGAGGATATTTTCACGAGGGATATGGTGGAGAAATTGGCCGCCTGA

Protein sequence

MDPSLIAGDVQETTKKKATRKRKSSDPKNQPLKVVYISNPMRVQTSAAEFRALVQELTGRDADFPDPTKFPSPSVHALETDPPKKPEEEDDDNDNDDELLLLDLAAMEDSSSDDFLEGSYGTLEDIFTRDMVEKLAA

Homology

[Close]

BLAST of Spg029018 vs. NCBI nr
Match: XP_023539403.1 (uncharacterized protein LOC111800056 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 127.1 bits (318), Expect = 1.2e-25
Identity = 81/137 (59.12%), Postives = 98/137 (71.53%), Query Frame = 0


Query: 1   MDPSLIAGDVQETTKKKATRKRKSSDPKNQPLKVVYISNPMRVQTSAAEFRALVQELTGR 60
           M+ SL+  DV+E  +K+  RKRK  D KNQPLKVVYISNPMRVQ SAAEFR LVQ+LTG+
Sbjct: 1   MESSLVGRDVEE-GRKERRRKRKPCDRKNQPLKVVYISNPMRVQASAAEFRGLVQQLTGQ 60

Query: 61  DADFPDPTKFPSPSVHALETDPPKKPEEEDDDNDNDDELLLLDLAAMEDSSSDDFLEGSY 120
           DA    P   PS SV   ET+  K    +DDD+D+D +LLL   AA+ + SSDDFL GSY
Sbjct: 61  DA----PKFPPSASVETPETNVRK---IQDDDDDDDQQLLLHWAAAIMEDSSDDFLGGSY 120

Query: 121 GTLEDIFTRDMVEKLAA 138
            +LE IF RDMV++LAA
Sbjct: 121 ESLEGIFMRDMVDRLAA 129

[Close]

BLAST of Spg029018 vs. NCBI nr
Match: XP_011652185.1 (uncharacterized protein LOC105434994 [Cucumis sativus])

HSP 1 Score: 121.7 bits (304), Expect = 4.9e-24
Identity = 74/140 (52.86%), Postives = 92/140 (65.71%), Query Frame = 0


Query: 1   MDPSLIAGDVQETTKK--KATRKRKSSDPKNQPLKVVYISNPMRVQTSAAEFRALVQELT 60
           MD SL+  ++  T KK  K TRKRKS +   QPLKVVYISNPMRV TSA+EFRALVQELT
Sbjct: 1   MDRSLLVSELTTTMKKNTKTTRKRKSCEENKQPLKVVYISNPMRVHTSASEFRALVQELT 60

Query: 61  GRDADFPDPTKF-PSPSVHALETDPPKKPEEEDDDNDNDDELLLLDLAAMEDSSSDDFLE 120
           GRDA+FPDPTKF P+ S   +  D  +K     +  +++ ELL+      + S  DDFL 
Sbjct: 61  GRDAEFPDPTKFYPASSCEIMNDDDVEKKVVAAEGEEDEQELLI------DSSCDDDFLR 120

Query: 121 GSYGTLEDIFTRDMVEKLAA 138
            SY +LEDI  RD++E   A
Sbjct: 121 SSYESLEDILRRDVMENFGA 134

[Close]

BLAST of Spg029018 vs. NCBI nr
Match: XP_022941301.1 (uncharacterized protein LOC111446639 [Cucurbita moschata])

HSP 1 Score: 119.8 bits (299), Expect = 1.9e-23
Identity = 81/137 (59.12%), Postives = 93/137 (67.88%), Query Frame = 0


Query: 1   MDPSLIAGDVQETTKKKATRKRKSSDPKNQPLKVVYISNPMRVQTSAAEFRALVQELTGR 60
           M+ SL+  DV+E  +K+  R RK  D KNQPLKVVYISNPMRVQ SAAEFR LVQELTG+
Sbjct: 1   MESSLVGRDVEE-GRKERRRNRKPCDRKNQPLKVVYISNPMRVQASAAEFRGLVQELTGQ 60

Query: 61  DADFPDPTKFPSPSVHALETDPPKKPEEEDDDNDNDDELLLLDLAAMEDSSSDDFLEGSY 120
           DA      KFP PS  +     P+    +  D D+DDE LLL  AAMED SSD FL GSY
Sbjct: 61  DA-----PKFP-PSASSASVQTPETNVRKIQD-DDDDEQLLLHWAAMED-SSDGFLGGSY 120

Query: 121 GTLEDIFTRDMVEKLAA 138
            + E IF RDMV+KLAA
Sbjct: 121 ESSEGIFMRDMVDKLAA 128

[Close]

BLAST of Spg029018 vs. NCBI nr
Match: XP_008443611.1 (PREDICTED: uncharacterized protein LOC103487167 [Cucumis melo] >KAA0053883.1 sigma factor binding protein 2 [Cucumis melo var. makuwa] >TYK25522.1 sigma factor binding protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 118.6 bits (296), Expect = 4.1e-23
Identity = 73/139 (52.52%), Postives = 91/139 (65.47%), Query Frame = 0


Query: 1   MDPSLIAGDVQETT--KKKATRKRKSSDPKNQPLKVVYISNPMRVQTSAAEFRALVQELT 60
           MD +L+  ++  TT  KKK TRKRKS +   QPLKVVYISNPMRV TSA+EFRALVQELT
Sbjct: 1   MDRTLLVSELTTTTTKKKKTTRKRKSCEENKQPLKVVYISNPMRVHTSASEFRALVQELT 60

Query: 61  GRDADFPDPTKFPSPSVHALETDPPKKPEEEDDDNDNDDELLLLDLAAMEDSSSDDFLEG 120
           GRDA+FPDP+KF   S   +  D  KK     D  ++D++ LL+D      S  DDF   
Sbjct: 61  GRDAEFPDPSKFYPASCEIVMNDLEKKVVA--DQGEDDEQELLID-----SSCDDDFWRS 120

Query: 121 SYGTLEDIFTRDMVEKLAA 138
           S+ +LEDI  RD++E   A
Sbjct: 121 SFESLEDILRRDVMENFGA 132

[Close]

BLAST of Spg029018 vs. NCBI nr
Match: XP_022147780.1 (sigma factor binding protein 2, chloroplastic-like [Momordica charantia])

HSP 1 Score: 117.9 bits (294), Expect = 7.1e-23
Identity = 70/132 (53.03%), Postives = 89/132 (67.42%), Query Frame = 0


Query: 1   MDPSLIAGDVQETTKKKATRKRKSSDPKNQPLKVVYISNPMRVQTSAAEFRALVQELTGR 60
           MD +LI  +++    KK TRKRK S  KNQPLKVVYISNPMRV TSA+EFRALVQELTGR
Sbjct: 1   MDRNLIVSEME--PMKKETRKRKLSVQKNQPLKVVYISNPMRVHTSASEFRALVQELTGR 60

Query: 61  DADFPDPTKFPSPSVHALETDPPKKPEEEDDDNDNDDELLLLDLAAMEDSSSDDFLEGSY 120
           DA+FPDP KF   S+   E +  K  E +DD+  +      LD+A   + S+DDFLE  Y
Sbjct: 61  DAEFPDPAKFHQASIDVGEMNLEKNREGDDDEEAS------LDVATTMEDSNDDFLESPY 120

Query: 121 GTLEDIFTRDMV 133
            + E++  R+M+
Sbjct: 121 ESFEEMLMREML 124

[Close]

BLAST of Spg029018 vs. ExPASy Swiss-Prot
Match: Q9LDH1 (Sigma factor binding protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIB1 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 4.6e-09
Identity = 34/68 (50.00%), Postives = 45/68 (66.18%), Query Frame = 0


Query: 16 KKATRKRKSSDPKNQPLKVVYISNPMRVQTSAAEFRALVQELTGRDADFPDPTKFPSPSV 75
          +K+ +++K +   N+P+KV YISNPMRVQT A++FR LVQELTG+DA    P    SPS 
Sbjct: 24 RKSPKQKKKTTSTNKPIKVRYISNPMRVQTCASKFRELVQELTGQDAVDLQPEPIYSPSS 83

Query: 76 HALETDPP 84
                PP
Sbjct: 84 DDHNLSPP 91

[Close]

BLAST of Spg029018 vs. ExPASy Swiss-Prot
Match: O80669 (Sigma factor binding protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIB2 PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 2.5e-07
Identity = 30/59 (50.85%), Postives = 43/59 (72.88%), Query Frame = 0


Query: 20 RKRKSSDPKNQPLKVVYISNPMRVQTSAAEFRALVQELTGRD-ADF-PDPTKFPSPSVH 77
          ++++ S   ++P+KV YISNPMRV+T  ++FR LVQELTG+D AD  P PT F +  +H
Sbjct: 25 KQKRKSTTTHKPIKVRYISNPMRVETCPSKFRELVQELTGQDAADLPPSPTTFTAVDLH 83

[Close]

BLAST of Spg029018 vs. ExPASy TrEMBL
Match: A0A0A0LFH5 (VQ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G820480 PE=4 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 2.4e-24
Identity = 74/140 (52.86%), Postives = 92/140 (65.71%), Query Frame = 0


Query: 1   MDPSLIAGDVQETTKK--KATRKRKSSDPKNQPLKVVYISNPMRVQTSAAEFRALVQELT 60
           MD SL+  ++  T KK  K TRKRKS +   QPLKVVYISNPMRV TSA+EFRALVQELT
Sbjct: 1   MDRSLLVSELTTTMKKNTKTTRKRKSCEENKQPLKVVYISNPMRVHTSASEFRALVQELT 60

Query: 61  GRDADFPDPTKF-PSPSVHALETDPPKKPEEEDDDNDNDDELLLLDLAAMEDSSSDDFLE 120
           GRDA+FPDPTKF P+ S   +  D  +K     +  +++ ELL+      + S  DDFL 
Sbjct: 61  GRDAEFPDPTKFYPASSCEIMNDDDVEKKVVAAEGEEDEQELLI------DSSCDDDFLR 120

Query: 121 GSYGTLEDIFTRDMVEKLAA 138
            SY +LEDI  RD++E   A
Sbjct: 121 SSYESLEDILRRDVMENFGA 134

[Close]

BLAST of Spg029018 vs. ExPASy TrEMBL
Match: A0A6J1FKR4 (uncharacterized protein LOC111446639 OS=Cucurbita moschata OX=3662 GN=LOC111446639 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 9.0e-24
Identity = 81/137 (59.12%), Postives = 93/137 (67.88%), Query Frame = 0


Query: 1   MDPSLIAGDVQETTKKKATRKRKSSDPKNQPLKVVYISNPMRVQTSAAEFRALVQELTGR 60
           M+ SL+  DV+E  +K+  R RK  D KNQPLKVVYISNPMRVQ SAAEFR LVQELTG+
Sbjct: 1   MESSLVGRDVEE-GRKERRRNRKPCDRKNQPLKVVYISNPMRVQASAAEFRGLVQELTGQ 60

Query: 61  DADFPDPTKFPSPSVHALETDPPKKPEEEDDDNDNDDELLLLDLAAMEDSSSDDFLEGSY 120
           DA      KFP PS  +     P+    +  D D+DDE LLL  AAMED SSD FL GSY
Sbjct: 61  DA-----PKFP-PSASSASVQTPETNVRKIQD-DDDDEQLLLHWAAMED-SSDGFLGGSY 120

Query: 121 GTLEDIFTRDMVEKLAA 138
            + E IF RDMV+KLAA
Sbjct: 121 ESSEGIFMRDMVDKLAA 128

[Close]

BLAST of Spg029018 vs. ExPASy TrEMBL
Match: A0A5D3DPQ4 (Sigma factor binding protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006730 PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 2.0e-23
Identity = 73/139 (52.52%), Postives = 91/139 (65.47%), Query Frame = 0


Query: 1   MDPSLIAGDVQETT--KKKATRKRKSSDPKNQPLKVVYISNPMRVQTSAAEFRALVQELT 60
           MD +L+  ++  TT  KKK TRKRKS +   QPLKVVYISNPMRV TSA+EFRALVQELT
Sbjct: 1   MDRTLLVSELTTTTTKKKKTTRKRKSCEENKQPLKVVYISNPMRVHTSASEFRALVQELT 60

Query: 61  GRDADFPDPTKFPSPSVHALETDPPKKPEEEDDDNDNDDELLLLDLAAMEDSSSDDFLEG 120
           GRDA+FPDP+KF   S   +  D  KK     D  ++D++ LL+D      S  DDF   
Sbjct: 61  GRDAEFPDPSKFYPASCEIVMNDLEKKVVA--DQGEDDEQELLID-----SSCDDDFWRS 120

Query: 121 SYGTLEDIFTRDMVEKLAA 138
           S+ +LEDI  RD++E   A
Sbjct: 121 SFESLEDILRRDVMENFGA 132

[Close]

BLAST of Spg029018 vs. ExPASy TrEMBL
Match: A0A1S3B8H6 (uncharacterized protein LOC103487167 OS=Cucumis melo OX=3656 GN=LOC103487167 PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 2.0e-23
Identity = 73/139 (52.52%), Postives = 91/139 (65.47%), Query Frame = 0


Query: 1   MDPSLIAGDVQETT--KKKATRKRKSSDPKNQPLKVVYISNPMRVQTSAAEFRALVQELT 60
           MD +L+  ++  TT  KKK TRKRKS +   QPLKVVYISNPMRV TSA+EFRALVQELT
Sbjct: 1   MDRTLLVSELTTTTTKKKKTTRKRKSCEENKQPLKVVYISNPMRVHTSASEFRALVQELT 60

Query: 61  GRDADFPDPTKFPSPSVHALETDPPKKPEEEDDDNDNDDELLLLDLAAMEDSSSDDFLEG 120
           GRDA+FPDP+KF   S   +  D  KK     D  ++D++ LL+D      S  DDF   
Sbjct: 61  GRDAEFPDPSKFYPASCEIVMNDLEKKVVA--DQGEDDEQELLID-----SSCDDDFWRS 120

Query: 121 SYGTLEDIFTRDMVEKLAA 138
           S+ +LEDI  RD++E   A
Sbjct: 121 SFESLEDILRRDVMENFGA 132

[Close]

BLAST of Spg029018 vs. ExPASy TrEMBL
Match: A0A6J1D137 (sigma factor binding protein 2, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111016633 PE=4 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 3.4e-23
Identity = 70/132 (53.03%), Postives = 89/132 (67.42%), Query Frame = 0


Query: 1   MDPSLIAGDVQETTKKKATRKRKSSDPKNQPLKVVYISNPMRVQTSAAEFRALVQELTGR 60
           MD +LI  +++    KK TRKRK S  KNQPLKVVYISNPMRV TSA+EFRALVQELTGR
Sbjct: 1   MDRNLIVSEME--PMKKETRKRKLSVQKNQPLKVVYISNPMRVHTSASEFRALVQELTGR 60

Query: 61  DADFPDPTKFPSPSVHALETDPPKKPEEEDDDNDNDDELLLLDLAAMEDSSSDDFLEGSY 120
           DA+FPDP KF   S+   E +  K  E +DD+  +      LD+A   + S+DDFLE  Y
Sbjct: 61  DAEFPDPAKFHQASIDVGEMNLEKNREGDDDEEAS------LDVATTMEDSNDDFLESPY 120

Query: 121 GTLEDIFTRDMV 133
            + E++  R+M+
Sbjct: 121 ESFEEMLMREML 124

[Close]

BLAST of Spg029018 vs. TAIR 10
Match: AT3G56710.1 (sigma factor binding protein 1 )

HSP 1 Score: 62.4 bits (150), Expect = 3.3e-10
Identity = 34/68 (50.00%), Postives = 45/68 (66.18%), Query Frame = 0


Query: 16 KKATRKRKSSDPKNQPLKVVYISNPMRVQTSAAEFRALVQELTGRDADFPDPTKFPSPSV 75
          +K+ +++K +   N+P+KV YISNPMRVQT A++FR LVQELTG+DA    P    SPS 
Sbjct: 24 RKSPKQKKKTTSTNKPIKVRYISNPMRVQTCASKFRELVQELTGQDAVDLQPEPIYSPSS 83

Query: 76 HALETDPP 84
                PP
Sbjct: 84 DDHNLSPP 91

[Close]

BLAST of Spg029018 vs. TAIR 10
Match: AT2G41180.1 (VQ motif-containing protein )

HSP 1 Score: 56.6 bits (135), Expect = 1.8e-08
Identity = 30/59 (50.85%), Postives = 43/59 (72.88%), Query Frame = 0


Query: 20 RKRKSSDPKNQPLKVVYISNPMRVQTSAAEFRALVQELTGRD-ADF-PDPTKFPSPSVH 77
          ++++ S   ++P+KV YISNPMRV+T  ++FR LVQELTG+D AD  P PT F +  +H
Sbjct: 25 KQKRKSTTTHKPIKVRYISNPMRVETCPSKFRELVQELTGQDAADLPPSPTTFTAVDLH 83

The following BLAST results are available for this feature:

BLAST of Spg029018 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023539403.1	1.2e-25	59.12	uncharacterized protein LOC111800056 [Cucurbita pepo subsp. pepo]	[more]
XP_011652185.1	4.9e-24	52.86	uncharacterized protein LOC105434994 [Cucumis sativus]	[more]
XP_022941301.1	1.9e-23	59.12	uncharacterized protein LOC111446639 [Cucurbita moschata]	[more]
XP_008443611.1	4.1e-23	52.52	PREDICTED: uncharacterized protein LOC103487167 [Cucumis melo] >KAA0053883.1 sig...	[more]
XP_022147780.1	7.1e-23	53.03	sigma factor binding protein 2, chloroplastic-like [Momordica charantia]	[more]

BLAST of Spg029018 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LDH1	4.6e-09	50.00	Sigma factor binding protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
O80669	2.5e-07	50.85	Sigma factor binding protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of Spg029018 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFH5	2.4e-24	52.86	VQ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G820480 PE=4 SV=...	[more]
A0A6J1FKR4	9.0e-24	59.12	uncharacterized protein LOC111446639 OS=Cucurbita moschata OX=3662 GN=LOC1114466...	[more]
A0A5D3DPQ4	2.0e-23	52.52	Sigma factor binding protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3B8H6	2.0e-23	52.52	uncharacterized protein LOC103487167 OS=Cucumis melo OX=3656 GN=LOC103487167 PE=...	[more]
A0A6J1D137	3.4e-23	53.03	sigma factor binding protein 2, chloroplastic-like OS=Momordica charantia OX=367...	[more]

BLAST of Spg029018 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56710.1	3.3e-10	50.00	sigma factor binding protein 1	[more]
AT2G41180.1	1.8e-08	50.85	VQ motif-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008889	VQ	PFAM	PF05678	VQ	coord: 37..62 e-value: 1.7E-12 score: 46.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..33
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 59..97
None	No IPR available	PANTHER	PTHR33624:SF2	SIGMA FACTOR BINDING PROTEIN 1, CHLOROPLASTIC	coord: 12..135
IPR039335	Sigma factor binding protein 1/2	PANTHER	PTHR33624	SIGMA FACTOR BINDING PROTEIN 1, CHLOROPLASTIC	coord: 12..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg029018.1	Spg029018.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051091	positive regulation of DNA-binding transcription factor activity