Spg028942 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg028942
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Werner Syndrome-like exonuclease
Location	scaffold5: 15729237 .. 15729860 (+)
RNA-Seq Expression	Spg028942
Synteny	Spg028942

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATCACCATTGTTGACCACCAAGTTCCCTCCGACTCCCACAATTACTTCGACATCACTCTCGACTCCGACGAGCCGATTCTCACTCTCCTCACCACTTCACCATCCATGGTACAAGCTTGGATTTCTGAAACCGTAGCCACTCAAACTCCACCTCTCGTCGTCGGTCTCGACGTCGAGTGGCGCCCTAACAATCGCTCGGACGAAAACCCCGTCGCCACACTGCAACTCTGCAACGGCCGCCGATGCCTGATTTTTCAACTGATCCACGCACCTGAGATCCCTAAATCTCTGTTCGAGTTCCTTGATGACGAGTCGTTCACTTTCGTAGGAGTCGGAATCGACGAGGATGTTGAGAAGCTCTACTGTGACTACGAATTGAAAGTGAGAAAGAAGGTGGATCTGAGGAATCTGGCTGAGAATGTGATGGGAAGAAGTGATCTGAAGAACGCGGGATTGAAGGGATTGGCGAAAGAGATTTTGGGGAAGGAGATTCAGAAGCCGAAGAGCGTGACGATGAGTAGGTGGGATCAAGAATGGCTTAATCTGAATCAGATTAAGTATGCTTGTGTTGATGCATTTCTGTCCTTTGAGATTGGAAGGCGTTTATCTTCCAATTGA

mRNA sequence

ATGGCGATCACCATTGTTGACCACCAAGTTCCCTCCGACTCCCACAATTACTTCGACATCACTCTCGACTCCGACGAGCCGATTCTCACTCTCCTCACCACTTCACCATCCATGGTACAAGCTTGGATTTCTGAAACCGTAGCCACTCAAACTCCACCTCTCGTCGTCGGTCTCGACGTCGAGTGGCGCCCTAACAATCGCTCGGACGAAAACCCCGTCGCCACACTGCAACTCTGCAACGGCCGCCGATGCCTGATTTTTCAACTGATCCACGCACCTGAGATCCCTAAATCTCTGTTCGAGTTCCTTGATGACGAGTCGTTCACTTTCGTAGGAGTCGGAATCGACGAGGATGTTGAGAAGCTCTACTGTGACTACGAATTGAAAGTGAGAAAGAAGGTGGATCTGAGGAATCTGGCTGAGAATGTGATGGGAAGAAGTGATCTGAAGAACGCGGGATTGAAGGGATTGGCGAAAGAGATTTTGGGGAAGGAGATTCAGAAGCCGAAGAGCGTGACGATGAGTAGGTGGGATCAAGAATGGCTTAATCTGAATCAGATTAAGTATGCTTGTGTTGATGCATTTCTGTCCTTTGAGATTGGAAGGCGTTTATCTTCCAATTGA

Coding sequence (CDS)

ATGGCGATCACCATTGTTGACCACCAAGTTCCCTCCGACTCCCACAATTACTTCGACATCACTCTCGACTCCGACGAGCCGATTCTCACTCTCCTCACCACTTCACCATCCATGGTACAAGCTTGGATTTCTGAAACCGTAGCCACTCAAACTCCACCTCTCGTCGTCGGTCTCGACGTCGAGTGGCGCCCTAACAATCGCTCGGACGAAAACCCCGTCGCCACACTGCAACTCTGCAACGGCCGCCGATGCCTGATTTTTCAACTGATCCACGCACCTGAGATCCCTAAATCTCTGTTCGAGTTCCTTGATGACGAGTCGTTCACTTTCGTAGGAGTCGGAATCGACGAGGATGTTGAGAAGCTCTACTGTGACTACGAATTGAAAGTGAGAAAGAAGGTGGATCTGAGGAATCTGGCTGAGAATGTGATGGGAAGAAGTGATCTGAAGAACGCGGGATTGAAGGGATTGGCGAAAGAGATTTTGGGGAAGGAGATTCAGAAGCCGAAGAGCGTGACGATGAGTAGGTGGGATCAAGAATGGCTTAATCTGAATCAGATTAAGTATGCTTGTGTTGATGCATTTCTGTCCTTTGAGATTGGAAGGCGTTTATCTTCCAATTGA

Protein sequence

MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTTSPSMVQAWISETVATQTPPLVVGLDVEWRPNNRSDENPVATLQLCNGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVGVGIDEDVEKLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSVTMSRWDQEWLNLNQIKYACVDAFLSFEIGRRLSSN

Homology

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BLAST of Spg028942 vs. NCBI nr
Match: XP_011652174.1 (Werner Syndrome-like exonuclease [Cucumis sativus] >KGN59430.1 hypothetical protein Csa_001395 [Cucumis sativus])

HSP 1 Score: 348.6 bits (893), Expect = 3.7e-92
Identity = 166/206 (80.58%), Postives = 185/206 (89.81%), Query Frame = 0


Query: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTTSPSMVQAWISETVATQTPPLVVGLDV 60
           MAITIVDH+VPSDSHNYFDIT D+DEPILTLLTTSPSMV  WIS+T+A QTPPL+VGLD+
Sbjct: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60

Query: 61  EWRPNNRSDENPVATLQLCNGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVGVGIDEDVE 120
           EWRPNNRS +NPVATLQLC GRRCLI QL+H PEIPKSLFEFL++ESFTFVGVGIDED E
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSVTMSRWDQE 180
           KL CDY LKV K++DLR+LAE+V GR +LKNAGLK L KE+LGKEI KPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLNLNQIKYACVDAFLSFEIGRRLSS 207
           WL LNQ+KYAC+DAF SFEIG+ L S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGKFLQS 206

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BLAST of Spg028942 vs. NCBI nr
Match: XP_008443244.1 (PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo] >ADN33882.1 3'-5' exonuclease [Cucumis melo subsp. melo] >KAA0053896.1 Werner Syndrome-like exonuclease [Cucumis melo var. makuwa] >TYK25510.1 Werner Syndrome-like exonuclease [Cucumis melo var. makuwa])

HSP 1 Score: 346.3 bits (887), Expect = 1.8e-91
Identity = 165/206 (80.10%), Postives = 184/206 (89.32%), Query Frame = 0


Query: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTTSPSMVQAWISETVATQTPPLVVGLDV 60
           M+ITIVDH+VPSDSHNYFDIT D+DEPILTLLTTSPSMV  WIS+T+  QTPPL+VGLD+
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNNRSDENPVATLQLCNGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVGVGIDEDVE 120
           EWRPNNRS +NPVATLQLC GRRCLI QL+H PEIPKSLFEFL++ESFTFVGVGIDED E
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSVTMSRWDQE 180
           KL CDY LKV K++DLR+LAE+V GR +LKNAGLK L KE+LGKEI KPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLNLNQIKYACVDAFLSFEIGRRLSS 207
           WL LNQ+KYAC+DAF SFEIGR L S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQS 206

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BLAST of Spg028942 vs. NCBI nr
Match: XP_038903327.1 (Werner Syndrome-like exonuclease isoform X2 [Benincasa hispida])

HSP 1 Score: 324.7 bits (831), Expect = 5.8e-85
Identity = 158/187 (84.49%), Postives = 169/187 (90.37%), Query Frame = 0


Query: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTTSPSMVQAWISETVATQTPPLVVGLDV 60
           MAITIVDHQVPSDSHNYFDITLDSDEPILTLLT SPSMV+ WISET+A QTPPL+VGLD+
Sbjct: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTNSPSMVENWISETLALQTPPLMVGLDI 60

Query: 61  EWRPNNRSDENPVATLQLCNGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVGVGIDEDVE 120
           EWRPNNRS +NPVATLQLC GRRCLI QLIH PEIPKSLFEFL++ES TFVGVGIDED E
Sbjct: 61  EWRPNNRSYDNPVATLQLCMGRRCLILQLIHTPEIPKSLFEFLENESLTFVGVGIDEDAE 120

Query: 121 KLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSVTMSRWDQE 180
           KL CDY LKV K+VDLRNLAE+V GR DLKNAGLK L KE+LGKEI KPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRVDLRNLAESVTGRRDLKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLNLNQI 188
           WL LNQ+
Sbjct: 181 WLTLNQM 187

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BLAST of Spg028942 vs. NCBI nr
Match: XP_038903326.1 (Werner Syndrome-like exonuclease isoform X1 [Benincasa hispida])

HSP 1 Score: 323.9 bits (829), Expect = 9.8e-85
Identity = 158/186 (84.95%), Postives = 168/186 (90.32%), Query Frame = 0


Query: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTTSPSMVQAWISETVATQTPPLVVGLDV 60
           MAITIVDHQVPSDSHNYFDITLDSDEPILTLLT SPSMV+ WISET+A QTPPL+VGLD+
Sbjct: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTNSPSMVENWISETLALQTPPLMVGLDI 60

Query: 61  EWRPNNRSDENPVATLQLCNGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVGVGIDEDVE 120
           EWRPNNRS +NPVATLQLC GRRCLI QLIH PEIPKSLFEFL++ES TFVGVGIDED E
Sbjct: 61  EWRPNNRSYDNPVATLQLCMGRRCLILQLIHTPEIPKSLFEFLENESLTFVGVGIDEDAE 120

Query: 121 KLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSVTMSRWDQE 180
           KL CDY LKV K+VDLRNLAE+V GR DLKNAGLK L KE+LGKEI KPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRVDLRNLAESVTGRRDLKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLNLNQ 187
           WL LNQ
Sbjct: 181 WLTLNQ 186

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BLAST of Spg028942 vs. NCBI nr
Match: KAG6580879.1 (Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017635.1 Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 323.9 bits (829), Expect = 9.8e-85
Identity = 156/207 (75.36%), Postives = 178/207 (85.99%), Query Frame = 0


Query: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTTSPSMVQAWISETVATQTPPLVVGLDV 60
           MA+TIVD + PS SHNYFD+T +SDEPILTLLT SP MV  WISET+ TQTPPL+VGLD+
Sbjct: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTPPLIVGLDI 60

Query: 61  EWRPNNRSDENPVATLQLCNGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVGVGIDEDVE 120
           EWRPN RS ENPVATLQLC GRRCLI QLI+APE PKSL+EFL++ES +FVGVGI+ D E
Sbjct: 61  EWRPNIRSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDAE 120

Query: 121 KLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSVTMSRWDQE 180
           KL CDY LKVRK VDLRNLAE+V GR +LKNAGLK LAKEILGKEI+KP+ VT+SRWDQE
Sbjct: 121 KLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQE 180

Query: 181 WLNLNQIKYACVDAFLSFEIGRRLSSN 208
           WL  NQIKYAC DAF+S+EIGR L ++
Sbjct: 181 WLTHNQIKYACFDAFVSYEIGRHLQAS 207

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BLAST of Spg028942 vs. ExPASy Swiss-Prot
Match: Q84LH3 (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 4.4e-19
Identity = 53/148 (35.81%), Postives = 85/148 (57.43%), Query Frame = 0


Query: 56  VGLDVEWRPNNRSDENP--VATLQLC-NGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVG 115
           VGLD+EWRP+ R    P  VAT+Q+C +   C +  + H+  IP+SL   ++D +   VG
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHS-GIPQSLQHLIEDSTLVKVG 189

Query: 116 VGIDEDVEKLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSV 175
           +GID D  KL+ DY + ++   DL +LA   +G    K  GL  L + ++ KE+ KP  +
Sbjct: 190 IGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGLASLTETLVCKELLKPNRI 249

Query: 176 TMSRWDQEWLNLNQIKYACVDAFLSFEI 201
            +  W+   L+  Q++YA  DA+ S+ +
Sbjct: 250 RLGNWEFYPLSKQQLQYAATDAYASWHL 274

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BLAST of Spg028942 vs. ExPASy Swiss-Prot
Match: Q14191 (Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2)

HSP 1 Score: 86.7 bits (213), Expect = 3.5e-16
Identity = 52/152 (34.21%), Postives = 82/152 (53.95%), Query Frame = 0


Query: 55  VVGLDVEWRP-NNRSDENPVATLQLC-NGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVG 114
           VVG D+EW P  NR     VA +QLC +  +C +F +      P+ L   L++++    G
Sbjct: 78  VVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAG 137

Query: 115 VGIDEDVEKLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSV 174
           VGI+ D  KL  D+++K++  V+L ++A   +  ++     L  L K +LGK++ K KS+
Sbjct: 138 VGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTE--TWSLNSLVKHLLGKQLLKDKSI 197

Query: 175 TMSRWDQEWLNLNQIKYACVDAFLSFEIGRRL 205
             S W +  L  +Q  YA  DA+  F I R L
Sbjct: 198 RCSNWSKFPLTEDQKLYAATDAYAGFIIYRNL 227

[Close]

BLAST of Spg028942 vs. ExPASy Swiss-Prot
Match: O93530 (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 7.7e-16
Identity = 51/152 (33.55%), Postives = 78/152 (51.32%), Query Frame = 0


Query: 55  VVGLDVEWRP-NNRSDENPVATLQLC-NGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVG 114
           V+G D+EW P   +     VA +Q+C + ++C +F +      PK L   L+DES   VG
Sbjct: 72  VLGFDIEWPPVYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVG 131

Query: 115 VGIDEDVEKLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSV 174
           VGI+ D  KL  DYELK++  ++L  +A   +   +       GL K +  +++ K KS 
Sbjct: 132 VGIEGDQWKLMSDYELKLKGFIELSEMANQKLRCKE--KWTFNGLIKHLFKEQLYKRKSY 191

Query: 175 TMSRWDQEWLNLNQIKYACVDAFLSFEIGRRL 205
             S WD   L  +Q  YA  DA+    I ++L
Sbjct: 192 RCSNWDIFLLTEDQKLYAATDAYAGLLIYKKL 221

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BLAST of Spg028942 vs. ExPASy Swiss-Prot
Match: O09053 (Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn PE=1 SV=3)

HSP 1 Score: 84.0 bits (206), Expect = 2.2e-15
Identity = 50/154 (32.47%), Postives = 82/154 (53.25%), Query Frame = 0


Query: 55  VVGLDVEWRPNNR-SDENPVATLQLC-NGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVG 114
           VVG D+EW P  +    + VA +QLC +  +C +F +      P+ L   L+++S    G
Sbjct: 72  VVGFDMEWPPIYKPGKRSRVAVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIKKAG 131

Query: 115 VGIDEDVEKLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSV 174
           VGI+ D  KL  D+++K+   V+L ++A   +  ++     L GL K +LGK++ K KS+
Sbjct: 132 VGIEGDQWKLLRDFDVKLESFVELTDVANEKLKCAE--TWSLNGLVKHVLGKQLLKDKSI 191

Query: 175 TMSRWDQEWLNLNQIKYACVDAFLSFEIGRRLSS 207
             S W    L  +Q  YA  DA+    I ++L +
Sbjct: 192 RCSNWSNFPLTEDQKLYAATDAYAGLIIYQKLGN 223

[Close]

BLAST of Spg028942 vs. ExPASy Swiss-Prot
Match: Q9VGN7 (Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227 GN=Exd2 PE=2 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 3.2e-14
Identity = 57/151 (37.75%), Postives = 78/151 (51.66%), Query Frame = 0


Query: 55  VVGLDVEWRPNNRSDENPVATLQLCNGR-RCLIFQLIHAPEIPKSLFEFLDDESFTFVGV 114
           V+G D EW     S   PVA LQL + R  C +F+L H  +IP+ L E L+D+S   VGV
Sbjct: 79  VLGFDCEWITVGGS-RRPVALLQLSSHRGLCALFRLCHMKQIPQDLRELLEDDSVIKVGV 138

Query: 115 GIDEDVEKLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSVT 174
              ED  KL  DY + V   +DLR L   VM  +  K  GL  L+K  L   + K   + 
Sbjct: 139 APQEDAMKLSHDYGVGVASTLDLRFLC--VM--AGHKPEGLGKLSKTHLNYTLDKHWRLA 198

Query: 175 MSRWDQEWLNLNQIKYACVDAFLSFEIGRRL 205
            S W+ + L   Q+ YA  DA ++  I ++L
Sbjct: 199 CSNWEAKTLEPKQLDYAANDALMAVAIYQKL 224

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BLAST of Spg028942 vs. ExPASy TrEMBL
Match: A0A0A0LBN3 (3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G819870 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.8e-92
Identity = 166/206 (80.58%), Postives = 185/206 (89.81%), Query Frame = 0


Query: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTTSPSMVQAWISETVATQTPPLVVGLDV 60
           MAITIVDH+VPSDSHNYFDIT D+DEPILTLLTTSPSMV  WIS+T+A QTPPL+VGLD+
Sbjct: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60

Query: 61  EWRPNNRSDENPVATLQLCNGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVGVGIDEDVE 120
           EWRPNNRS +NPVATLQLC GRRCLI QL+H PEIPKSLFEFL++ESFTFVGVGIDED E
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSVTMSRWDQE 180
           KL CDY LKV K++DLR+LAE+V GR +LKNAGLK L KE+LGKEI KPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLNLNQIKYACVDAFLSFEIGRRLSS 207
           WL LNQ+KYAC+DAF SFEIG+ L S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGKFLQS 206

[Close]

BLAST of Spg028942 vs. ExPASy TrEMBL
Match: A0A5A7UI20 (Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006600 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 9.0e-92
Identity = 165/206 (80.10%), Postives = 184/206 (89.32%), Query Frame = 0


Query: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTTSPSMVQAWISETVATQTPPLVVGLDV 60
           M+ITIVDH+VPSDSHNYFDIT D+DEPILTLLTTSPSMV  WIS+T+  QTPPL+VGLD+
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNNRSDENPVATLQLCNGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVGVGIDEDVE 120
           EWRPNNRS +NPVATLQLC GRRCLI QL+H PEIPKSLFEFL++ESFTFVGVGIDED E
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSVTMSRWDQE 180
           KL CDY LKV K++DLR+LAE+V GR +LKNAGLK L KE+LGKEI KPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLNLNQIKYACVDAFLSFEIGRRLSS 207
           WL LNQ+KYAC+DAF SFEIGR L S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQS 206

[Close]

BLAST of Spg028942 vs. ExPASy TrEMBL
Match: E5GBP0 (3'-5' exonuclease OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 9.0e-92
Identity = 165/206 (80.10%), Postives = 184/206 (89.32%), Query Frame = 0


Query: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTTSPSMVQAWISETVATQTPPLVVGLDV 60
           M+ITIVDH+VPSDSHNYFDIT D+DEPILTLLTTSPSMV  WIS+T+  QTPPL+VGLD+
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNNRSDENPVATLQLCNGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVGVGIDEDVE 120
           EWRPNNRS +NPVATLQLC GRRCLI QL+H PEIPKSLFEFL++ESFTFVGVGIDED E
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSVTMSRWDQE 180
           KL CDY LKV K++DLR+LAE+V GR +LKNAGLK L KE+LGKEI KPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLNLNQIKYACVDAFLSFEIGRRLSS 207
           WL LNQ+KYAC+DAF SFEIGR L S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQS 206

[Close]

BLAST of Spg028942 vs. ExPASy TrEMBL
Match: A0A1S3B738 (Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103486879 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 9.0e-92
Identity = 165/206 (80.10%), Postives = 184/206 (89.32%), Query Frame = 0


Query: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTTSPSMVQAWISETVATQTPPLVVGLDV 60
           M+ITIVDH+VPSDSHNYFDIT D+DEPILTLLTTSPSMV  WIS+T+  QTPPL+VGLD+
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRPNNRSDENPVATLQLCNGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVGVGIDEDVE 120
           EWRPNNRS +NPVATLQLC GRRCLI QL+H PEIPKSLFEFL++ESFTFVGVGIDED E
Sbjct: 61  EWRPNNRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDAE 120

Query: 121 KLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSVTMSRWDQE 180
           KL CDY LKV K++DLR+LAE+V GR +LKNAGLK L KE+LGKEI KPKSVTMSRWDQE
Sbjct: 121 KLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQE 180

Query: 181 WLNLNQIKYACVDAFLSFEIGRRLSS 207
           WL LNQ+KYAC+DAF SFEIGR L S
Sbjct: 181 WLTLNQVKYACIDAFFSFEIGRFLQS 206

[Close]

BLAST of Spg028942 vs. ExPASy TrEMBL
Match: A0A6J1D107 (Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016613 PE=4 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 4.8e-85
Identity = 161/207 (77.78%), Postives = 179/207 (86.47%), Query Frame = 0


Query: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTTSPSMVQAWISETVATQTPPLVVGLDV 60
           MAI+IVDH++PSDSHN +D+T+DSD+ I TLLT+SP+MV +WISET+A QTPPL+VGLD+
Sbjct: 1   MAISIVDHEIPSDSHNLYDVTIDSDQ-IHTLLTSSPAMVDSWISETLAIQTPPLIVGLDI 60

Query: 61  EWRPN-NRSDENPVATLQLCNGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVGVGIDEDV 120
           EWRPN N S ENPVATLQLC  RRCLIFQLIHA EIP+SL +FL DESFTFVGVGI EDV
Sbjct: 61  EWRPNRNSSYENPVATLQLCFDRRCLIFQLIHASEIPRSLTDFLTDESFTFVGVGIGEDV 120

Query: 121 EKLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSVTMSRWDQ 180
           EKL CDY L V   VDLRNLAEN MGR DLKNAGLKGLAKE+LGKEI KPKSVTMSRWD 
Sbjct: 121 EKLLCDYGLNVSNTVDLRNLAENAMGRGDLKNAGLKGLAKEVLGKEISKPKSVTMSRWDN 180

Query: 181 EWLNLNQIKYACVDAFLSFEIGRRLSS 207
           EWL  NQ+KYACVDAFLS EIGR LS+
Sbjct: 181 EWLVPNQVKYACVDAFLSSEIGRHLSN 206

[Close]

BLAST of Spg028942 vs. TAIR 10
Match: AT2G36110.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 99.0 bits (245), Expect = 4.8e-21
Identity = 63/182 (34.62%), Postives = 99/182 (54.40%), Query Frame = 0


Query: 26  EPILTLLTTSPSMVQAWISE----TVATQTPPLVVGLDVEWRPNNRSDENPVATLQLCNG 85
           E ++  +T + S ++ WI      +    + PLVVGLDV+W P     + P   LQLC G
Sbjct: 24  ERLIVTVTHTTSTIRRWIHSIRFFSRLRSSHPLVVGLDVQWTPG--GSDPPPDILQLCVG 83

Query: 86  RRCLIFQLIHAPEIPKSLFEFLDDESFTFVGVGIDEDVEKL-YCDYELKVRKKVDLRNLA 145
            RCLI QL H   IP+ L  FL+DE+ TFVGV   +D  KL    ++L++ + +D+R+  
Sbjct: 84  NRCLIIQLSHCKRIPEVLRSFLEDETITFVGVWNSQDQGKLERFRHQLEIWRLLDIRHYL 143

Query: 146 ENVMGRSDLKNAGLKGLAKEILG-KEIQKPKSVTMSRWDQEWLNLNQIKYACVDAFLSFE 202
                 + L N+  + + +E LG K ++K K + MS W    L+ +QI  A  D ++  +
Sbjct: 144 P-----TRLLNSSFEKIVEECLGYKGVRKDKEICMSNWGARSLSHDQIVQASDDVYVCCK 198

[Close]

BLAST of Spg028942 vs. TAIR 10
Match: AT3G12410.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 8.2e-21
Identity = 66/212 (31.13%), Postives = 110/212 (51.89%), Query Frame = 0


Query: 13  DSHNYFDITLDSDEPILTLLTTSPSMVQAWISETVAT---QTPPLVVGLDVEW------- 72
           ++H  + +    DE I+T +T   S++  WI   +      + PLVVG+ V+W       
Sbjct: 13  NTHLEYSVDFFGDEFIVT-VTWDSSVISRWIRNVLFNNRFSSHPLVVGVGVQWTPFSYYS 72

Query: 73  --RPNN---------RSDENPVATLQLCNGRRCLIFQLIHAPEIPKSLFEFLDDESFTFV 132
             RPNN            +NP   LQLC G RCLI QL +  ++P +L  FL D   TFV
Sbjct: 73  DPRPNNYYADPPPIRYYSDNPADILQLCVGNRCLIIQLGYCDQVPNNLRSFLADPETTFV 132

Query: 133 GVGIDEDVEKL-YCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKE-IQKP 192
           GV   +D  KL  C ++L++ + +D+R    +  GRS ++ +  + + +E +G + +   
Sbjct: 133 GVWNGQDAGKLARCCHQLEIGELLDIRRYVTDSWGRS-MRRSSFEEIVEECMGYQGVMLD 192

Query: 193 KSVTMSRWDQEWLNLNQIKYACVDAFLSFEIG 202
             ++MS W    L+L+QI  A +DA++  ++G
Sbjct: 193 PEISMSDWTAYDLDLDQILQASLDAYVCHQLG 222

[Close]

BLAST of Spg028942 vs. TAIR 10
Match: AT4G13870.1 (Werner syndrome-like exonuclease )

HSP 1 Score: 97.1 bits (240), Expect = 1.8e-20
Identity = 54/152 (35.53%), Postives = 87/152 (57.24%), Query Frame = 0


Query: 56  VGLDVEWRPNNRSDENP--VATLQLC-NGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVG 115
           VGLD+EWRP+ R    P  VAT+Q+C +   C +  + H+  IP+SL   ++D +   VG
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHS-GIPQSLQHLIEDSTLVKVG 189

Query: 116 VGIDEDVEKLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSV 175
           +GID D  KL+ DY + ++   DL +LA   +G    K  GL  L + ++ KE+ KP  +
Sbjct: 190 IGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGLASLTETLVCKELLKPNRI 249

Query: 176 TMSRWDQEWLNLNQIKYACVDAFLSFEIGRRL 205
            +  W+   L+  Q++YA  DA+ S+ + + L
Sbjct: 250 RLGNWEFYPLSKQQLQYAATDAYASWHLYKDL 278

[Close]

BLAST of Spg028942 vs. TAIR 10
Match: AT4G13870.2 (Werner syndrome-like exonuclease )

HSP 1 Score: 96.3 bits (238), Expect = 3.1e-20
Identity = 53/148 (35.81%), Postives = 85/148 (57.43%), Query Frame = 0


Query: 56  VGLDVEWRPNNRSDENP--VATLQLC-NGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVG 115
           VGLD+EWRP+ R    P  VAT+Q+C +   C +  + H+  IP+SL   ++D +   VG
Sbjct: 130 VGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHS-GIPQSLQHLIEDSTLVKVG 189

Query: 116 VGIDEDVEKLYCDYELKVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGKEIQKPKSV 175
           +GID D  KL+ DY + ++   DL +LA   +G    K  GL  L + ++ KE+ KP  +
Sbjct: 190 IGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGLASLTETLVCKELLKPNRI 249

Query: 176 TMSRWDQEWLNLNQIKYACVDAFLSFEI 201
            +  W+   L+  Q++YA  DA+ S+ +
Sbjct: 250 RLGNWEFYPLSKQQLQYAATDAYASWHL 274

[Close]

BLAST of Spg028942 vs. TAIR 10
Match: AT3G12470.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 92.8 bits (229), Expect = 3.4e-19
Identity = 62/193 (32.12%), Postives = 104/193 (53.89%), Query Frame = 0


Query: 14  SHNYFDITLDSDEPILTLLTTSPSMVQAWI----SETVATQTPPLVVGLDVEWRPNNRSD 73
           +H    +    D+ I+T +T + S+++ WI    S        PLVVG+ V+WRP++   
Sbjct: 13  THQKHFVDFFGDDLIVT-VTPTASVIRRWIRSVRSYNRNHSVHPLVVGIGVQWRPDSSDP 72

Query: 74  ENPVATLQLCNGRRCLIFQLIHAPEIPKSLFEFLDDESFTFVGVGIDEDVEKL-YCDYEL 133
           + P  TLQLC G RCLI QL +   +PK L  FL D   TFVGV   +D +KL  C + +
Sbjct: 73  DPPPKTLQLCVGSRCLIIQLGYNYGLPKVLRTFLADPKTTFVGVWNGQDQKKLEKCRHRV 132

Query: 134 KVRKKVDLRNLAENVMGRSDLKNAGLKGLAKEILGK-EIQKPKSVTMSRWDQEWLNLNQI 193
           ++ K +D+R    +  G + +     + + KE LG+  ++   ++ MS W    LN  Q+
Sbjct: 133 EIGKLLDIRMFVRDSRG-ARMCFCSFEQIVKERLGRVGVRLDPAICMSDWGVYNLNHYQV 192

Query: 194 KYACVDAFLSFEI 201
             A +++++ F++
Sbjct: 193 LQASIESYVCFKL 203

The following BLAST results are available for this feature:

BLAST of Spg028942 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011652174.1	3.7e-92	80.58	Werner Syndrome-like exonuclease [Cucumis sativus] >KGN59430.1 hypothetical prot...	[more]
XP_008443244.1	1.8e-91	80.10	PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo] >ADN33882.1 3'-5' exo...	[more]
XP_038903327.1	5.8e-85	84.49	Werner Syndrome-like exonuclease isoform X2 [Benincasa hispida]	[more]
XP_038903326.1	9.8e-85	84.95	Werner Syndrome-like exonuclease isoform X1 [Benincasa hispida]	[more]
KAG6580879.1	9.8e-85	75.36	Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. sororia...	[more]

BLAST of Spg028942 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84LH3	4.4e-19	35.81	Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=...	[more]
Q14191	3.5e-16	34.21	Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2	[more]
O93530	7.7e-16	33.55	Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn ...	[more]
O09053	2.2e-15	32.47	Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn P...	[more]
Q9VGN7	3.2e-14	37.75	Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227...	[more]

BLAST of Spg028942 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LBN3	1.8e-92	80.58	3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A5A7UI20	9.0e-92	80.10	Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
E5GBP0	9.0e-92	80.10	3'-5' exonuclease OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
A0A1S3B738	9.0e-92	80.10	Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103486879 PE=4 SV...	[more]
A0A6J1D107	4.8e-85	77.78	Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016613 ...	[more]

BLAST of Spg028942 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G36110.1	4.8e-21	34.62	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G12410.1	8.2e-21	31.13	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G13870.1	1.8e-20	35.53	Werner syndrome-like exonuclease	[more]
AT4G13870.2	3.1e-20	35.81	Werner syndrome-like exonuclease	[more]
AT3G12470.1	3.4e-19	32.12	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002562	3'-5' exonuclease domain	SMART	SM00474	35exoneu6	coord: 36..205 e-value: 1.6E-4 score: 31.0
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 37..204 e-value: 8.9E-19 score: 67.9
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 18..206 e-value: 1.0E-58 score: 200.1
None	No IPR available	PANTHER	PTHR13620:SF65	OS01G0660800 PROTEIN	coord: 9..206
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 9..206
None	No IPR available	CDD	cd06141	WRN_exo	coord: 33..204 e-value: 2.46617E-63 score: 192.023
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 48..204

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg028942.1	Spg028942.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006139	nucleobase-containing compound metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding