Spg028842 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg028842
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Cytochrome b561 domain-containing protein
Location	scaffold7: 16212014 .. 16216088 (-)
RNA-Seq Expression	Spg028842
Synteny	Spg028842

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCAGTGAGTGGAGTAATCCAAGGTAAACCGATATCTATAAGAATCATTAAAAAAAAGAGAGATTATATTGATGGACAATAAGTACGTATTAAAATATGAATCGTTAGCTATATATTACTTAAACTTCTATAAGAATGTATAGAACTAATCTTAATTGGTTTTAAATTCATTTTAGTTCATAATATTTTTCTCAAATTATTACACGTGCAACGCACGTGGTAAAAACCTAGTTTATTTAAAAAGGTGAAATGCTTATTTGTTAATTTAAATATTTTTTAAAACCTTTTTATTTATATGAAATTTTAAGAAATATTTGTCGATAGTGATATTTTATTAATATTTCTATCTGAAACTTTCATAAAATTGAAACTTCAAAATTACCCTATGCACATCAACATTGCTGTTAAATGTCAAAATTTTGCAATGGTTAAACTTTAATTAAAAAATAAATAAATAAAATAAAATTGCATTGACTCAGTCAAATCATTCATGTGATGATGTATCAGTATTACACAAACAAATAGTCATTGATTCTATCAGAGTTAAATGCTTAGACATTGTCTTTTCCTCTAATTCCTTTGTGAAAACATCAAGATTATGAAAGAAGACAACCAATCATCTTAGCCATTGCTTGTTTATAATTTCTAGAAAATATAAATTAGTGCTGTTTGGCTTTTGAGTTCTCTCTGTCCCCCTTAAAATTATTTATTTCTCAATTTTTCTCATGTATTTTTACTAAGCATTGACAGTCTGGAATGCAATTTTAATAAGTATAGTTAATTTATATTTAAATACAAATTTTTTACTCTAAAAAAATACTAATTCAAATAACCCAATTTAAATCTGCCTAAGCTTAATCCAAAAATAACACGACCCGTATGTATATATTATTAGATTACATGGAATTTCATCTCAAATCAATTGGCAATGAGTGATGTAGTCCATGTATCTTATAAATGGTGAGGTCTCCACTTATCTTTTCAATGTGGGATCCTCAACATATATTATAGCTTATGAGGGACATTGTTTGCATTCTCGATAGAACTTTCGATAGAAAAGTATTTGTCAATTGTATTAATTTTTCTAAATGAATTATTGGTTAAAAATACTTTCGATAGAAAAGTATTTGTCATTGTTTGCAGTCTCCATTTTTTCAAATATCCAAATTATGTCACTGTACTTTCAATTTCAATAAGACCTAAAGAGGTTATCGTTAATTTGCCAATATTAATATATCACGTAAGTATTGAAAGCACATTATTTGTGACGTTCAAGTATAGGAGAGCTACATGAATGAACTGATACCACATCCAAATGAGAGGGACCATGAGGATATGAGAGTATGGTCAATATTAGCTTAAAGAATTAGTAATAATTTTCTATACCAATAAGGTGCATCTTTCTTCTCAGTGGCTCAATCATAGCAACTTCAAAGTTAAGCGTGCGTGACTCTAGGAAGTTTTATATTTGGATGACATGCTAAGATTTTTCCTAAGAAGCATGTTATAAGGATCTCACGTTGGTCTGTGGGGACAATCTTCACTCTTAGAAGCACATTAGGATAAGTATGGTGACGATCATGCCAAGTCATAGGACATAGAGGAAAATGTCGGGACCGTCAGGTACCGAATCCTATAAGCTTGTAATTTATATGCAAAATTCGAAAATATATAGTTAAGTAATATAGTACGTAGAGCCCGTGGGAGCAATTAAACGACAGATTTTTCTTGCACGCGGAGAGCTGCCGGCCTCAGGCGGTGGGTCTGGCTTTGTTATAAACAAAGTCCAATCGGAGACACCAACATATATTTCCTTCTTCTTTCCTCTCTCTATATATATATCTGCCAATACAATTGTACTTCTCAAAATCTCTCAAACTCTTTCTTTCTCTTTCTTTGTACAGAGCTTTTTGAGGCCATTTCAATGGCGTTCAAACGAGGCAACTTTCAGATAATGGCGAGGCCAGTCACTTTGTTGGGTCATTTGGTGGCCATGGCTGTGGCTGCTTTGATTCTTGTTTGGATCCTCCATTTCAGAGAAGGGGTCGCCTTCAAATCAGATAACAAGCTCAAGATTCTAAATGTAATTCTCTTTCAATTTCTTTTGATCGAGGGTATAGTGTTGAAAAATTTCTATATCGAAAGAGAACGACTTTACTTTCACTAACTCTTTTAGGTTGAATTACGTATATATTTTGATTTAGTTTATATTGATTTTTTATCGAGTTTAAAAGTTTTGATTTAGTCCTTTTGATTTGGTTCAAATATAAAATATAAACGGATTGTCACAAAAATATAAAAAAAATGTGTCATGTGTTCAATATGATAATGGAAGTGATAGTTTGTGAGTGTGTGACTAACTTTAGATAAAAACTTCAAAATCGAAGGAATTAATCCAATTGAAACTAGCTAAGTTCAAACCATAGTGACTAAATTTGTAATATTTAAATCATTTTGTATATTTTATTATTTTTCATTTCTAAAAATTTAAGAGGTAAGTAGAAGAAAACTTTTCCATATTAATTTTTATGTAATGTTTTTGAATTTTTTTCCTTCTTTGCTCTTGCCCATATATATCAATTTGATTTGCAACTGCAGTTTCTTCATCAAAGGTTTGATGAAATTTTCTTTGTTGTTTTTCAGCTGCATGTAGTTTTAATGGTGGTTGGACTCATCTTGTTTGGAGGACAAGGTATTGGGAAAAAAAAAAACATATATTTATCCTTTAGCTATTTTCTTTTTCAGAATAAGATATATATTACACAAATTTGAGAGTAGGTTTTGAATTTAAAAAGAAGTTACAATATCTTAAGTAATTGAGTTGACATTCTATGTCTTAATATATACAAAGTTTGTTCATGTGAAAGAGTTTATTGGAAATATAACTTAATGAAGCTTCAATATTACTAAATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACATTTTGTCAATGATTACATAGAAAAAAATAGAATATATATTTTAATAATAATTATTATAACATATCATTGATTACACCATTAATGAATTGGTTGATATATTAGTAGGTATAGTTAATCAATTCAAACATTAAGCCTAACTTAAAAATTAAAACATCTTTACATCCTCTAATGGATCATAGATTCAAATTTTCATTTGCACCTATGAAGTAATATTCTAAAAAAGAATTTACATTTAATCAATGTGTAAGATAGGTATACATCATAATTGATTACTAATGTTATAGTTAGTCAATTTATATATACATGTATATCAACAATTACCGTTAGTTAATATATACTATAAAATTATTAAAAGGATATTTTAACATTATCGTTTTTCACCATCGACCTTTAAGATGATAATTGATATCTTATCTAATTAACTATAAATGCTCTAAGATTGTTGTATGGTTTTGTGGAGCAGCTATAATGGCATACAAGTCAATACCAGCACCAAAATATTGGAGAAAAGTGGTGCATTTGATATTGCATATAGTAGCATTAGCAGCTGGAGTTTTTGGGGTTTGTTTGGCTTTCAAGTTCCACCATGAGACCAATATTTCAGATCTTCTTTCATTACACTCTTGGCTTGGTTTGATCACCATTTGCTTGTACGGTCTTCAGGTTTGTCCCAACGACCTCTCCCTTCATTCCTTTTTTTTGAACAATACTTAGCTATATATACCGCTATCACCACTAAAATATCGACAAAGTTTTAATGCGGGAGATCCTTATACACTGTTGTGCAATCAGGTATGGTTCTTTTTCTTAGCTTAAAGATAATGAAGTTAATTAATAATTATTTATATATATGTATGAAACACACGGTGGATATTTGGATTCTTTGCCTACTTCTTCCCTGGGGCAGAATCAAGAAGGAGAGCAAGCATAATGCCATGGCACATTTACTTTGGAATATTCATCTTCTTAATTGCAACATGCACTGCTGAGTTAGGGTTGCTTCAAAGGTTCAACTCTTTGTTCTTAGGGCATTCACAAGAAGGCCTTATTGTCAACTTCACTGGCCTTCTCATTCTTCTCTATGCTGCCACTGTTGTTCTCACTGTCATTCTTCCACCCCTTTATTAA

mRNA sequence

GCAGTGAGTGGAGTAATCCAAGAGCTTTTTGAGGCCATTTCAATGGCGTTCAAACGAGGCAACTTTCAGATAATGGCGAGGCCAGTCACTTTGTTGGGTCATTTGGTGGCCATGGCTGTGGCTGCTTTGATTCTTGTTTGGATCCTCCATTTCAGAGAAGGGGTCGCCTTCAAATCAGATAACAAGCTCAAGATTCTAAATCTGCATGTAGTTTTAATGGTGGTTGGACTCATCTTGTTTGGAGGACAAGCTATAATGGCATACAAGTCAATACCAGCACCAAAATATTGGAGAAAAGTGGTGCATTTGATATTGCATATAGTAGCATTAGCAGCTGGAGTTTTTGGGGTTTGTTTGGCTTTCAAGTTCCACCATGAGACCAATATTTCAGATCTTCTTTCATTACACTCTTGGCTTGGTTTGATCACCATTTGCTTGTACGGTCTTCAGTTAGGGTTGCTTCAAAGGTTCAACTCTTTGTTCTTAGGGCATTCACAAGAAGGCCTTATTGTCAACTTCACTGGCCTTCTCATTCTTCTCTATGCTGCCACTGTTGTTCTCACTGTCATTCTTCCACCCCTTTATTAA

Coding sequence (CDS)

GCAGTGAGTGGAGTAATCCAAGAGCTTTTTGAGGCCATTTCAATGGCGTTCAAACGAGGCAACTTTCAGATAATGGCGAGGCCAGTCACTTTGTTGGGTCATTTGGTGGCCATGGCTGTGGCTGCTTTGATTCTTGTTTGGATCCTCCATTTCAGAGAAGGGGTCGCCTTCAAATCAGATAACAAGCTCAAGATTCTAAATCTGCATGTAGTTTTAATGGTGGTTGGACTCATCTTGTTTGGAGGACAAGCTATAATGGCATACAAGTCAATACCAGCACCAAAATATTGGAGAAAAGTGGTGCATTTGATATTGCATATAGTAGCATTAGCAGCTGGAGTTTTTGGGGTTTGTTTGGCTTTCAAGTTCCACCATGAGACCAATATTTCAGATCTTCTTTCATTACACTCTTGGCTTGGTTTGATCACCATTTGCTTGTACGGTCTTCAGTTAGGGTTGCTTCAAAGGTTCAACTCTTTGTTCTTAGGGCATTCACAAGAAGGCCTTATTGTCAACTTCACTGGCCTTCTCATTCTTCTCTATGCTGCCACTGTTGTTCTCACTGTCATTCTTCCACCCCTTTATTAA

Protein sequence

AVSGVIQELFEAISMAFKRGNFQIMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMVVGLILFGGQAIMAYKSIPAPKYWRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLSLHSWLGLITICLYGLQLGLLQRFNSLFLGHSQEGLIVNFTGLLILLYAATVVLTVILPPLY

Homology

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BLAST of Spg028842 vs. NCBI nr
Match: XP_022150036.1 (probable ascorbate-specific transmembrane electron transporter 1 [Momordica charantia])

HSP 1 Score: 279.3 bits (713), Expect = 2.6e-71
Identity = 153/222 (68.92%), Postives = 167/222 (75.23%), Query Frame = 0


Query: 15  MAFKRGNFQIMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMV 74
           MA KRG FQI+ARPVT+LGH+VAMAVAALIL WILHFREGVA KS NKLKILNLHVVLMV
Sbjct: 1   MASKRGKFQILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMV 60

Query: 75  VGLILFGGQAIMAYKSIPAPKYWRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLS 134
           VG ILFGG+AIMAYKS+P  KY+RK+VHL+LHI ALA G+FGVCLAFKFHH+T++SDLLS
Sbjct: 61  VGFILFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLS 120

Query: 135 LHSWLGLITICLYGLQ-----------------------------------------LGL 194
           LHSW GLITI LY LQ                                         LGL
Sbjct: 121 LHSWFGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGL 180

Query: 195 LQRFNSLFLGHSQEGLIVNFTGLLILLYAATVVLTVILPPLY 196
           LQRFN+L LGHSQEGLIVNFTGLL+LLYAATVVLTVILPPLY
Sbjct: 181 LQRFNTLILGHSQEGLIVNFTGLLLLLYAATVVLTVILPPLY 222

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BLAST of Spg028842 vs. NCBI nr
Match: XP_022941965.1 (probable ascorbate-specific transmembrane electron transporter 1 [Cucurbita moschata])

HSP 1 Score: 265.4 bits (677), Expect = 3.9e-67
Identity = 146/223 (65.47%), Postives = 166/223 (74.44%), Query Frame = 0


Query: 15  MAFKRGNFQIMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMV 74
           MAFKR NF IMARPVTLLGHLVA+++A LILVWILHFREGVAFKSDNKLKILNLHV+LMV
Sbjct: 1   MAFKR-NFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMV 60

Query: 75  VGLILFGGQAIMAYKSIPAPKYWRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLS 134
           +GLILFGGQAIMAYKSIPA KYWRKVVHL LH +ALAA +FG+C++FKFHH+  +++L+S
Sbjct: 61  IGLILFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVS 120

Query: 135 LHSWLGLITICLYGLQ-----------------------------------------LGL 194
           LHSW GLIT+CLYGLQ                                         LGL
Sbjct: 121 LHSWFGLITVCLYGLQWVVGFFTYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGL 180

Query: 195 LQRFNSLF-LGHSQEGLIVNFTGLLILLYAATVVLTVILPPLY 196
           LQ  ++ F LGH+QE LI+NFTGLLI LYAATVVLTVILPPLY
Sbjct: 181 LQSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 222

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BLAST of Spg028842 vs. NCBI nr
Match: KAG6599883.1 (Transmembrane ascorbate ferrireductase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030568.1 Transmembrane ascorbate ferrireductase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 265.0 bits (676), Expect = 5.1e-67
Identity = 145/223 (65.02%), Postives = 166/223 (74.44%), Query Frame = 0


Query: 15  MAFKRGNFQIMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMV 74
           MAFKR NF IMARPVTLLGHLVA+++A LILVWILHFREGVAFKSDNKLKILNLHV+LMV
Sbjct: 1   MAFKR-NFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMV 60

Query: 75  VGLILFGGQAIMAYKSIPAPKYWRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLS 134
           +GL+LFGGQAIMAYKSIPA KYWRKVVHL LH +ALAA +FG+C++FKFHH+  +++L+S
Sbjct: 61  IGLVLFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVS 120

Query: 135 LHSWLGLITICLYGLQ-----------------------------------------LGL 194
           LHSW GLIT+CLYGLQ                                         LGL
Sbjct: 121 LHSWFGLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGL 180

Query: 195 LQRFNSLF-LGHSQEGLIVNFTGLLILLYAATVVLTVILPPLY 196
           LQ  ++ F LGH+QE LI+NFTGLLI LYAATVVLTVILPPLY
Sbjct: 181 LQSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 222

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BLAST of Spg028842 vs. NCBI nr
Match: KAE8648229.1 (hypothetical protein Csa_018523 [Cucumis sativus])

HSP 1 Score: 261.9 bits (668), Expect = 4.3e-66
Identity = 142/218 (65.14%), Postives = 159/218 (72.94%), Query Frame = 0


Query: 19  RGNFQIMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMVVGLI 78
           +GN +IMARPVTLLGHLVA+A+A LILVWI HF  GVA KSDNKLKILNLHV+LM +G I
Sbjct: 3   KGNLKIMARPVTLLGHLVAIAIATLILVWIFHFSGGVAIKSDNKLKILNLHVILM-IGFI 62

Query: 79  LFGGQAIMAYKSIPAPKYWRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLSLHSW 138
           LFGGQAIMAYKSIP  KYWRKVVHL+LH++ALA G+FGVC+AFKFHHE  +SDLLSLHSW
Sbjct: 63  LFGGQAIMAYKSIPGRKYWRKVVHLVLHLMALACGIFGVCMAFKFHHEIKLSDLLSLHSW 122

Query: 139 LGLITICLYGLQ-----------------------------------------LGLLQRF 196
           +GLI + LY  Q                                         LGLLQRF
Sbjct: 123 IGLIAVSLYAFQWIFGFFVYFFPGAEARRRGNMLPWHVYFGVFIFLIATCNAELGLLQRF 182

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BLAST of Spg028842 vs. NCBI nr
Match: XP_022987904.1 (probable ascorbate-specific transmembrane electron transporter 1 [Cucurbita maxima])

HSP 1 Score: 261.9 bits (668), Expect = 4.3e-66
Identity = 145/223 (65.02%), Postives = 164/223 (73.54%), Query Frame = 0


Query: 15  MAFKRGNFQIMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMV 74
           MAFKR NF IMARPVTLLGHLVA+++A LILVWILHFREGVAFKSDNKLKILNLHV+LMV
Sbjct: 1   MAFKR-NFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMV 60

Query: 75  VGLILFGGQAIMAYKSIPAPKYWRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLS 134
           +GLILFGGQAIMAYKSIPA KYWRKVVHL LH +ALAA +FG+C++FKFH +  + +L+S
Sbjct: 61  IGLILFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHRDIQLKNLVS 120

Query: 135 LHSWLGLITICLYGLQ-----------------------------------------LGL 194
           LHSW GLIT+CLYGLQ                                         LGL
Sbjct: 121 LHSWFGLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGL 180

Query: 195 LQRFNSLF-LGHSQEGLIVNFTGLLILLYAATVVLTVILPPLY 196
           LQ  ++ F LGH+QE LI+NFTGLLI LYAATVVLTVILPPLY
Sbjct: 181 LQSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 222

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BLAST of Spg028842 vs. ExPASy Swiss-Prot
Match: A3A9H6 (Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0642300 PE=3 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.1e-31
Identity = 81/215 (37.67%), Postives = 119/215 (55.35%), Query Frame = 0


Query: 24  IMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMVVGLILFGGQ 83
           + A P T + H +A+A A ++LVW +HFR G+AF++ NK  I N+H VLM++G I+ G +
Sbjct: 5   VRAAPFTYVAHALAVAAATMVLVWCIHFRGGLAFEATNKNLIFNVHPVLMLIGYIILGSE 64

Query: 84  AIMAYKSIPAPKY-WRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLSLHSWLGLI 143
           AIM YK +P  K+   K++HLILH +AL  G  G+  AFKFH+E+ I++L SLHSWLG+ 
Sbjct: 65  AIMVYKVLPTWKHDTTKLIHLILHAIALVFGAVGIYCAFKFHNESGIANLYSLHSWLGIG 124

Query: 144 TICLYGLQ-----------------------------------------LGLLQRFNSLF 193
           TICLYG+Q                                         LG L++   L 
Sbjct: 125 TICLYGIQWIFGFVAFFFPRASPSVRKGVLPWHILFGLFVYILALATAELGFLEKLTFLQ 184

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BLAST of Spg028842 vs. ExPASy Swiss-Prot
Match: Q8L856 (Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB561A PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 4.7e-31
Identity = 79/211 (37.44%), Postives = 119/211 (56.40%), Query Frame = 0


Query: 23  QIMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMVVGLILFGG 82
           +I A  VT + H +A+  A ++LVW + +R G+A+++ NK  I NLH VLM++G I+ GG
Sbjct: 4   RINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFIILGG 63

Query: 83  QAIMAYKSIPAPKYWRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLSLHSWLGLI 142
           +AI++YKS+P  K  +K++HLILH +ALA G+FG+C AFK H+E++I +L SLHSW+G+ 
Sbjct: 64  EAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWIGIG 123

Query: 143 TICLYGLQ-----------------------------------------LGLLQRFNSLF 189
            I LYG Q                                         LG L++   L 
Sbjct: 124 VISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLE 183

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BLAST of Spg028842 vs. ExPASy Swiss-Prot
Match: Q7XMK3 (Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0533500 PE=2 SV=2)

HSP 1 Score: 131.7 bits (330), Expect = 8.8e-30
Identity = 80/215 (37.21%), Postives = 116/215 (53.95%), Query Frame = 0


Query: 24  IMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMVVGLILFGGQ 83
           + A P T + H +A+A A ++LVW + FR G+AF++DNK  I N+H VLM++G I+ G +
Sbjct: 7   VKAAPFTYVAHALAVAAAVMVLVWCISFRGGLAFEADNKNLIFNVHPVLMLIGYIILGSE 66

Query: 84  AIMAYKSIPAPKY-WRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLSLHSWLGLI 143
           AIM YK  P   +   K++HLILH +A+  G  G+  AFKFH+E+ I++L SLHSWLG+ 
Sbjct: 67  AIMIYKIFPKLNHDTTKLIHLILHAIAIVLGAVGIYCAFKFHNESGIANLYSLHSWLGIG 126

Query: 144 TICLYGLQ-----------------------------------------LGLLQRFNSLF 193
           TI LYG+Q                                         LGLL++   L 
Sbjct: 127 TISLYGIQWIFGFVAFFYPGAAPHVRRGALPWHVLFGLFVYVLTLATAELGLLEKLTFLQ 186

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BLAST of Spg028842 vs. ExPASy Swiss-Prot
Match: C4IYS8 (Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 6.3e-28
Identity = 76/215 (35.35%), Postives = 116/215 (53.95%), Query Frame = 0


Query: 24  IMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMVVGLILFGGQ 83
           + A P T   H +A+A AA++LVW ++FR G+A ++ NK  I N+H VLM++G I+ GG+
Sbjct: 7   VRAAPFTYAAHALAVAAAAMVLVWAIYFRGGLAIEATNKNLIFNVHPVLMLIGYIIIGGE 66

Query: 84  AIMAYKSIPAPKY-WRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLSLHSWLGLI 143
           AIM Y+ +P   +   K++HL+LH +AL  G  G+  AFK H+E+ I++L SLHSW+G+ 
Sbjct: 67  AIMVYRVLPTSNHETNKLIHLVLHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIG 126

Query: 144 TICLYGLQ-----------------------------------------LGLLQRFNSLF 193
           TI LYG+Q                                         LG L++   L 
Sbjct: 127 TITLYGIQWIVGFVTFFFPGAAPNVKKGVLPWHILFGLFVYILALANAELGFLEKLTFLE 186

[Close]

BLAST of Spg028842 vs. ExPASy Swiss-Prot
Match: Q6I681 (Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=ZCYB PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 8.3e-28
Identity = 77/215 (35.81%), Postives = 115/215 (53.49%), Query Frame = 0


Query: 24  IMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMVVGLILFGGQ 83
           + A P T   H +A+A AA++LVW + FR G+A +S NK  I N+H VLM++G ++ GG+
Sbjct: 7   VRAAPFTYAAHALAVAAAAMVLVWSIQFRGGLAIESTNKNLIFNVHPVLMLIGYVIIGGE 66

Query: 84  AIMAYKSIPAPKY-WRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLSLHSWLGLI 143
           AIM Y+ +P   +   K++HLILH +AL  G  G+  AFK H+E+ I++L SLHSW+G+ 
Sbjct: 67  AIMVYRVLPTSNHDTTKLIHLILHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIG 126

Query: 144 TICLYGLQ-----------------------------------------LGLLQRFNSLF 193
           TI LYG+Q                                         LG L++   L 
Sbjct: 127 TITLYGIQWIIGFVTFFFPGAAPNVKKGVLPWHVLFGLFVYILALANAELGFLEKLTFLE 186

[Close]

BLAST of Spg028842 vs. ExPASy TrEMBL
Match: A0A6J1DA84 (probable ascorbate-specific transmembrane electron transporter 1 OS=Momordica charantia OX=3673 GN=LOC111018308 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 1.3e-71
Identity = 153/222 (68.92%), Postives = 167/222 (75.23%), Query Frame = 0


Query: 15  MAFKRGNFQIMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMV 74
           MA KRG FQI+ARPVT+LGH+VAMAVAALIL WILHFREGVA KS NKLKILNLHVVLMV
Sbjct: 1   MASKRGKFQILARPVTILGHVVAMAVAALILAWILHFREGVALKSANKLKILNLHVVLMV 60

Query: 75  VGLILFGGQAIMAYKSIPAPKYWRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLS 134
           VG ILFGG+AIMAYKS+P  KY+RK+VHL+LHI ALA G+FGVCLAFKFHH+T++SDLLS
Sbjct: 61  VGFILFGGEAIMAYKSLPGKKYYRKLVHLVLHITALACGIFGVCLAFKFHHDTHLSDLLS 120

Query: 135 LHSWLGLITICLYGLQ-----------------------------------------LGL 194
           LHSW GLITI LY LQ                                         LGL
Sbjct: 121 LHSWFGLITISLYALQWVFGFFAYFFPGAESRRRASIMPWHIYFGIFIFLMAICTAELGL 180

Query: 195 LQRFNSLFLGHSQEGLIVNFTGLLILLYAATVVLTVILPPLY 196
           LQRFN+L LGHSQEGLIVNFTGLL+LLYAATVVLTVILPPLY
Sbjct: 181 LQRFNTLILGHSQEGLIVNFTGLLLLLYAATVVLTVILPPLY 222

[Close]

BLAST of Spg028842 vs. ExPASy TrEMBL
Match: A0A6J1FVA1 (probable ascorbate-specific transmembrane electron transporter 1 OS=Cucurbita moschata OX=3662 GN=LOC111447173 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.9e-67
Identity = 146/223 (65.47%), Postives = 166/223 (74.44%), Query Frame = 0


Query: 15  MAFKRGNFQIMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMV 74
           MAFKR NF IMARPVTLLGHLVA+++A LILVWILHFREGVAFKSDNKLKILNLHV+LMV
Sbjct: 1   MAFKR-NFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMV 60

Query: 75  VGLILFGGQAIMAYKSIPAPKYWRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLS 134
           +GLILFGGQAIMAYKSIPA KYWRKVVHL LH +ALAA +FG+C++FKFHH+  +++L+S
Sbjct: 61  IGLILFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHHDIQLTNLVS 120

Query: 135 LHSWLGLITICLYGLQ-----------------------------------------LGL 194
           LHSW GLIT+CLYGLQ                                         LGL
Sbjct: 121 LHSWFGLITVCLYGLQWVVGFFTYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGL 180

Query: 195 LQRFNSLF-LGHSQEGLIVNFTGLLILLYAATVVLTVILPPLY 196
           LQ  ++ F LGH+QE LI+NFTGLLI LYAATVVLTVILPPLY
Sbjct: 181 LQSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 222

[Close]

BLAST of Spg028842 vs. ExPASy TrEMBL
Match: A0A6J1JK51 (probable ascorbate-specific transmembrane electron transporter 1 OS=Cucurbita maxima OX=3661 GN=LOC111485304 PE=4 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 2.1e-66
Identity = 145/223 (65.02%), Postives = 164/223 (73.54%), Query Frame = 0


Query: 15  MAFKRGNFQIMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMV 74
           MAFKR NF IMARPVTLLGHLVA+++A LILVWILHFREGVAFKSDNKLKILNLHV+LMV
Sbjct: 1   MAFKR-NFNIMARPVTLLGHLVAISIAVLILVWILHFREGVAFKSDNKLKILNLHVLLMV 60

Query: 75  VGLILFGGQAIMAYKSIPAPKYWRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLS 134
           +GLILFGGQAIMAYKSIPA KYWRKVVHL LH +ALAA +FG+C++FKFH +  + +L+S
Sbjct: 61  IGLILFGGQAIMAYKSIPAKKYWRKVVHLGLHAIALAAAIFGICMSFKFHRDIQLKNLVS 120

Query: 135 LHSWLGLITICLYGLQ-----------------------------------------LGL 194
           LHSW GLIT+CLYGLQ                                         LGL
Sbjct: 121 LHSWFGLITVCLYGLQWVVGFFAYFFPGAESRRRASLMPWHVYFGIFVFLMATCTAELGL 180

Query: 195 LQRFNSLF-LGHSQEGLIVNFTGLLILLYAATVVLTVILPPLY 196
           LQ  ++ F LGH+QE LI+NFTGLLI LYAATVVLTVILPPLY
Sbjct: 181 LQSLDTWFLLGHTQENLIINFTGLLIFLYAATVVLTVILPPLY 222

[Close]

BLAST of Spg028842 vs. ExPASy TrEMBL
Match: A0A6P4B0Q4 (probable ascorbate-specific transmembrane electron transporter 1 OS=Ziziphus jujuba OX=326968 GN=LOC107429205 PE=4 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 1.7e-44
Identity = 104/219 (47.49%), Postives = 138/219 (63.01%), Query Frame = 0


Query: 15  MAFKRGNFQIMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMV 74
           MA K  ++QI A PVT+L HL+ +A+  L+LVW+LHFREG AFKS NK KI NLH   M+
Sbjct: 1   MAPKSRSYQISATPVTVLAHLLVIAITTLVLVWLLHFREGFAFKSINKEKIFNLHPFFMI 60

Query: 75  VGLILFGGQAIMAYKSIPAPKYWRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLS 134
           +G IL GG+AIMAYKS+P  +  +K+ H+I H++AL AG+ G+   FK+ HE  +SD+++
Sbjct: 61  IGFILVGGEAIMAYKSVPGRRKAQKLEHIIFHLIALLAGILGIYAVFKYKHEAGLSDMVT 120

Query: 135 LHSWLGLITICLYGLQ-----------------------------------------LGL 193
           LHSWLG+ITI LYGLQ                                          GL
Sbjct: 121 LHSWLGIITISLYGLQYLLGFFTYFFPGAEMSTRGNFLPWHVFVGILIFVLAICTAETGL 180

[Close]

BLAST of Spg028842 vs. ExPASy TrEMBL
Match: A0A5D2LYG7 (Cytochrome b561 domain-containing protein OS=Gossypium tomentosum OX=34277 GN=ES332_D02G173400v1 PE=4 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 1.7e-44
Identity = 108/221 (48.87%), Postives = 139/221 (62.90%), Query Frame = 0


Query: 15  MAFKRGNFQIMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMV 74
           MA K  ++QI A PVT+ GHL+ +AV  L+LVW+L FREG+AF+S NK KI NLH +LMV
Sbjct: 1   MAPKSRSYQISATPVTVFGHLLFIAVTTLVLVWLLKFREGLAFESANKFKIFNLHPLLMV 60

Query: 75  VGLILFGGQAIMAYKSIPAPK--YWRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDL 134
           +G IL  G+AIMAYKS P+ +    +K VHL L  +AL  G+FG+ + FKFH+ETN+ D+
Sbjct: 61  IGFILMAGEAIMAYKSTPSRRDIQVQKAVHLTLQTIALGCGIFGIVVIFKFHNETNMPDM 120

Query: 135 LSLHSWLGLITICLYGLQ-----------------------------------------L 193
           ++LHSWLG+ITICL+GLQ                                         +
Sbjct: 121 VTLHSWLGMITICLFGLQYLLGFFSYVFPGAESYSRAAYLPWHTFGGLLIFFLAICTAEM 180

[Close]

BLAST of Spg028842 vs. TAIR 10
Match: AT4G25570.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 136.0 bits (341), Expect = 3.3e-32
Identity = 79/211 (37.44%), Postives = 119/211 (56.40%), Query Frame = 0


Query: 23  QIMARPVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMVVGLILFGG 82
           +I A  VT + H +A+  A ++LVW + +R G+A+++ NK  I NLH VLM++G I+ GG
Sbjct: 4   RINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFIILGG 63

Query: 83  QAIMAYKSIPAPKYWRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLSLHSWLGLI 142
           +AI++YKS+P  K  +K++HLILH +ALA G+FG+C AFK H+E++I +L SLHSW+G+ 
Sbjct: 64  EAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWIGIG 123

Query: 143 TICLYGLQ-----------------------------------------LGLLQRFNSLF 189
            I LYG Q                                         LG L++   L 
Sbjct: 124 VISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLE 183

[Close]

BLAST of Spg028842 vs. TAIR 10
Match: AT1G14730.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 122.5 bits (306), Expect = 3.8e-28
Identity = 71/203 (34.98%), Postives = 104/203 (51.23%), Query Frame = 0


Query: 30  TLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMVVGLILFGGQAIMAYK 89
           TL+ H   +    L+L+W+LH+REG+ + SDN LK+LN+H  LM  G +   GQA+M YK
Sbjct: 18  TLVAHFFGILAVVLMLIWLLHYREGIEYGSDNPLKVLNVHPFLMYCGFLFLVGQAMMTYK 77

Query: 90  SIPAPKYWRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLSLHSWLGLITICLYGL 149
           +  A    +K+VH  LH++ L  G+ G+C AF+FH + N+ D++SLHSW+GL T  L G+
Sbjct: 78  TAYASHQVQKMVHGGLHLIGLVLGIVGICAAFRFHDKVNLKDMVSLHSWIGLTTFILLGV 137

Query: 150 Q-----------------------------------------LGLLQRFNSLFLGHSQEG 192
           Q                                          GL+QR   L    + E 
Sbjct: 138 QWLFGAFTFLAPQSSSGTRTRMMPWHVLGGRALLYMGIVAALTGLMQRATMLGQSTNAES 197

[Close]

BLAST of Spg028842 vs. TAIR 10
Match: AT5G38630.1 (cytochrome B561-1 )

HSP 1 Score: 112.5 bits (280), Expect = 3.9e-25
Identity = 70/211 (33.18%), Postives = 106/211 (50.24%), Query Frame = 0


Query: 28  PVTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMVVGLILFGGQAIMA 87
           P+ ++  ++   +AAL+L W +H+R G+A  SDNK  I N+H V+MV+GLILF G+A++A
Sbjct: 10  PIFMVVRVLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLILFNGEAMLA 69

Query: 88  YKSIPAPKYWRKVVHLILHIVALAAGVFGVCLAFKFHHETNISDLLSLHSWLGLITICLY 147
           YKS+   K  +K+VHL L + A    + GV  A KFH +  I +  SLHSWLGL  + L+
Sbjct: 70  YKSVQGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYSLHSWLGLACLFLF 129

Query: 148 GLQ-----------------------------------------LGLLQR-----FNSLF 193
             Q                                          G+L++      N + 
Sbjct: 130 AFQWAAGFVTYWYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKVTFLQVNQVI 189

[Close]

BLAST of Spg028842 vs. TAIR 10
Match: AT1G26100.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 72.8 bits (177), Expect = 3.5e-13
Identity = 44/123 (35.77%), Postives = 70/123 (56.91%), Query Frame = 0


Query: 29  VTLLGHLVAMAVAALILVWILHFREGVAFKSDNKLKILNLHVVLMVVGLILFGGQAIMAY 88
           + L   L  + VA  +L W L       F  +  L    LH +LMV+G IL  G+AI+ +
Sbjct: 11  LVLFARLSGLVVAVSVLYWAL-------FLPNLGLSYSTLHPLLMVIGFILVSGEAILIH 70

Query: 89  KSIPAPKYWRKVVHLILHIVALAAGVFGVCLAFKFHHETNI-SDLLSLHSWLGLITICLY 148
           + +P  +  +K VHL L  +ALA+ VFG+    KFH++  + ++  SLHSW+GL+++ L+
Sbjct: 71  RWLPGSRKTKKAVHLWLQGMALASAVFGIWT--KFHYQRGVFANFYSLHSWMGLLSVSLF 124

Query: 149 GLQ 151
             Q
Sbjct: 131 AAQ 124

The following BLAST results are available for this feature:

BLAST of Spg028842 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150036.1	2.6e-71	68.92	probable ascorbate-specific transmembrane electron transporter 1 [Momordica char...	[more]
XP_022941965.1	3.9e-67	65.47	probable ascorbate-specific transmembrane electron transporter 1 [Cucurbita mosc...	[more]
KAG6599883.1	5.1e-67	65.02	Transmembrane ascorbate ferrireductase 1, partial [Cucurbita argyrosperma subsp....	[more]
KAE8648229.1	4.3e-66	65.14	hypothetical protein Csa_018523 [Cucumis sativus]	[more]
XP_022987904.1	4.3e-66	65.02	probable ascorbate-specific transmembrane electron transporter 1 [Cucurbita maxi...	[more]

BLAST of Spg028842 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A3A9H6	2.1e-31	37.67	Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa...	[more]
Q8L856	4.7e-31	37.44	Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB5...	[more]
Q7XMK3	8.8e-30	37.21	Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa...	[more]
C4IYS8	6.3e-28	35.35	Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2...	[more]
Q6I681	8.3e-28	35.81	Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=Z...	[more]

BLAST of Spg028842 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DA84	1.3e-71	68.92	probable ascorbate-specific transmembrane electron transporter 1 OS=Momordica ch...	[more]
A0A6J1FVA1	1.9e-67	65.47	probable ascorbate-specific transmembrane electron transporter 1 OS=Cucurbita mo...	[more]
A0A6J1JK51	2.1e-66	65.02	probable ascorbate-specific transmembrane electron transporter 1 OS=Cucurbita ma...	[more]
A0A6P4B0Q4	1.7e-44	47.49	probable ascorbate-specific transmembrane electron transporter 1 OS=Ziziphus juj...	[more]
A0A5D2LYG7	1.7e-44	48.87	Cytochrome b561 domain-containing protein OS=Gossypium tomentosum OX=34277 GN=ES...	[more]

BLAST of Spg028842 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25570.1	3.3e-32	37.44	Cytochrome b561/ferric reductase transmembrane protein family	[more]
AT1G14730.1	3.8e-28	34.98	Cytochrome b561/ferric reductase transmembrane protein family	[more]
AT5G38630.1	3.9e-25	33.18	cytochrome B561-1	[more]
AT1G26100.1	3.5e-13	35.77	Cytochrome b561/ferric reductase transmembrane protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006593	Cytochrome b561/ferric reductase transmembrane	SMART	SM00665	561_7	coord: 68..183 e-value: 2.9E-12 score: 56.7
IPR006593	Cytochrome b561/ferric reductase transmembrane	PFAM	PF03188	Cytochrom_B561	coord: 68..151 e-value: 2.1E-23 score: 83.0
IPR006593	Cytochrome b561/ferric reductase transmembrane	PROSITE	PS50939	CYTOCHROME_B561	coord: 32..195 score: 25.167595
None	No IPR available	GENE3D	1.20.120.1770		coord: 22..157 e-value: 6.9E-41 score: 141.8
None	No IPR available	PANTHER	PTHR10106:SF43	CYTOCHROME B561 FAMILY PROTEIN, EXPRESSED	coord: 150..192
None	No IPR available	PANTHER	PTHR10106:SF43	CYTOCHROME B561 FAMILY PROTEIN, EXPRESSED	coord: 22..150
IPR043205	Cytochrome b561/Cytochrome b reductase 1-like	PANTHER	PTHR10106	CYTOCHROME B561-RELATED	coord: 22..150
IPR043205	Cytochrome b561/Cytochrome b reductase 1-like	PANTHER	PTHR10106	CYTOCHROME B561-RELATED	coord: 150..192

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg028842.1	Spg028842.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016491	oxidoreductase activity