Spg028821 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg028821
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Ribonuclease H
Location	scaffold7: 16040636 .. 16043742 (-)
RNA-Seq Expression	Spg028821
Synteny	Spg028821

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAGGTTGCAGCAGATGCTCCTTCCAAATGTCAGCTTCCCCTTCTTCTATGGAATCTCCTTCTCTTCCCTTCTCGATTTCTCCACTGCTGTCTGTATGTATCTCCTTCTCTTTTTTTGTTTCTTCTCCTTTTTATATCCTTCACAAATGTGGTTGCCCTAAAAAAAATATTCCTCTCTTCATTGAATTCAGGGCTACATCAGCTTCTTTTCAGCAAGATGGCAGACTCCCATTGGATGAGAGAGCTGATTGTAATAAAAAGTGCCAGCTCATTTAATGAACCGATTTTTCTCTCGTGTGAATCACGTGTGAACTTTGCTCCAGAATTGATCCAGCAATTCCATCTCCAGCTCCTCAATCACTTTAACTGCTACACATGCTGCCTTTAGTCTGCTACCAAAATGACTTTATGGATCTGCAATATAAGTACACAAATTTTAATGCATTTCACTTTCAAAACAGTAGAAAAATATTAACTATTTCCTCAAAGATGATTCAGACCAAAGCATGAGGTTTATAGAGGGAAAATATCAAAATATGCTAGCCAATTACTCCCATATTAAAGAATTCAAGCTAAAAGATAAGGTAAATAACTCTTATACTAAGAGTTATCAGCCGCCAAAGTCAATGTCGGGCATGTATGCAGAGAATGGTGGCAGGAAAAGGGCTCGAGCAGTGTCCAAGACCGAGCAGGGCCAGAAGCGATGAGAGCAGGAGCTGTCTCAGTGGTTGAAAGAAGAAGACAGCCATCGCGACTGCCAGAGGAGAGTTGAGGATGAAGACATAGAGGAGCTGATCGGGCACATGGAGCTGCCCTTCACCGACGAGATAATGGAGGGGGAAGTACCCCATATATTTAAGGTGTCGAACTTCCCACAGTACGACGTAAAGAAAGATCCAAAGCAGCACTTAGACTCGTACCTTACCTGGATGGATTTCCATGGGGCGAACGAAGCGACAAAGTGCCGCGCCTTCGCGTTAACGTTGACAGGTTTGGCGAGACAGTGGTTTAGCAAAATACCGCGCAGGTCAATTGGCTCGTTCAAAGATTTGGCACGCGTGTTCTTCACACAGTTCTTAGGGGCTCGAGAGCGACAGAAGCCACAAATCAATTTATTAACGGTCAAGCAAGGGCCGAGAGAAAGTTTAAAAGATTACGTTAACAGGTTTAGTAATGAGGTTTTGCAGGTAGAGGGGTACGATGATGGAGTCGCCCTAGCAGCTGTCATCTCGGGGTTACAGGATGAGAGGTTGTTGAATTCAATCGGAGAAAGCCAACCACGGACGTATGTGGAATTCATGACTCGGGCACAGAGGTTCATAAGCGCCGAGGAACTATTGAAATCTAAACAGGAAGAAAGGGAGAGCCGGGGAGTTTCGGTGTATGACCGACATAGAGAAGACAGAGGAAAAAGACGCCGGGTGGAAGACAGCGGCCGAGAGCGACACGGACACTCCTCAGCCAATGGCCGAGGCCGAGCAGAAATCAAAGAACTGCAAGGCAGGGCCGAGTCGAAAGCCAAGTTCAACAGGTACACACTGCTAACGGCTCCACTGGAACAGGTGTTAACCGCAATACAGGACACAAATCTGTTGAAGCGCCCGGAAAGGATGAGGTCAGATCCAGACAGGAGAAATAGAAGTAAATACTGCATGTTCCACGGGGACCACGACCACACAACCCGAGAATGCATACAATTGAGGGATGAAATAGAAACCCTAATTCGAGAAGGCTATCTCAAGGAATTCGTACAAGACAGAGGGAAGAGGCCAACACCAACAGACCAAGGTAAAGGAGGCAACAACCCCCCACTGGAGATACGAACCATCCTTGGAGGACCCTCGGGGGGGAGTCGAGTAGAAAGCGGAAAGCGGCAGTTCGAGAGGCTTGGATAGAGCCAACGGACCGAGGTATGTACTCGATCCTACTCAATAAAAACTCACCAAACTTAGAGTTTACAGAGGAGGAGGCCACGGGGATACACCACCCGCACAATGACGCACTGGTAATAGTTTTAACGATCGCCAATGCCAAAGTACATCAAATTTTGGTTGATGGAGGGAGCTCAGCCGACGTCCTCTCAGCCGCCGCATTCGATGCCATGAAACTGGGAAGCGACCGCCTGAGGCCAAACCTTACGCCGTTGGTGGGATTTGGAGGAGAAAAAGTAAACCCAAGGGGAAGCATCGAGCTGCCAGTAACCTTCGGGGAAGGACAGCAGACGGTAACCAGAATGATCAACTTCCTGGTAGTGGACTGCATTCCAGCATATAATGCCATTCTGAGGCGACCAACTTTGCATGGACTTAAGGCCGTGGCCTCTACATATCATCAAGTCCTGAAATTCCCAACTGAAGACGGTGTAGGAGAGGTGCGCGGAGAACAGAAGGTATCAAGATAATGTTATTTCATGGCGCTCAAGAGCATTAACAGGATAATACAAGCAACGCCCGCCTCAGGAAATGTCCGAGGCCGAGCACTTAAGGGGCCGAACCATCCCCTTCCAATGGAGCATTGACCTCAGCTTGTTATGTTAGCAAGTAACCAATTCATTAGAAATTATGTTCAACTTTCCTTTATTTCAGCAGCAAATAGTTTGGAATGTAGTAGGCCGATTCGCTTGAAATAAAATTAGCCCAAATCAATTAGCCCACAGCAGTCGATGCAGAGAAGACCCAAACTTATCGGGCCCACAGGCGGAAGAGGCTTAACCCACCAGACGACGGGCCGGGGTACGCTCCGGCCCCATATCCGATGATCCTCGGCCTAGGCCCAAGGGAGAGGCCGAGGGTCACGGTCGGCCTCGGCCCAAAGCCGAGGCCGACCAGGGCCAAAGGCCCGGGGCCACCTTCCGAAACGGGACGAGTTCTCAACCCTAGAAATCCCTATAAATGGGAAGGTCGCACACTCCCACAGGTATGCTAAAAACTACACCCTAAATACCTTCTACGGTTTTTCCGGCCAGAGCTAACTTAAGCATCGGAGTGTGTGTGGTCTACACCACACCGGTGTGCAGCGTTTGCTCGTCTTGCAGGTCACGTTTTTCCCGCTCATCTACAAATTCACTGTTGTCTGTCAAGTGGAGCGATGGGCAAATTCCTGA

mRNA sequence

ATGGAAAGGTTGCAGCAGATGCTCCTTCCAAATGTCAGCTTCCCCTTCTTCTATGGAATCTCCTTCTCTTCCCTTCTCGATTTCTCCACTGCTGTCTCCGCCAAAGTCAATGTCGGGCATGTATGCAGAGAATGGTGGCAGGAAAAGGGCTCGAGCAGTGTCCAAGACCGAGCAGGGCCAGAAGCGATGAGAGCAGGAGCTGTCTCAGATGAGAGGTTGTTGAATTCAATCGGAGAAAGCCAACCACGGACGTATGTGGAATTCATGACTCGGGCACAGAGGTTCATAAGCGCCGAGGAACTATTGAAATCTAAACAGGAAGAAAGGGAGAGCCGGGGAGTTTCGGTGTATGACCGACATAGAGAAGACAGAGGAAAAAGACGCCGGGTGGAAGACAGCGGCCGAGAGCGACACGGACACTCCTCAGCCAATGGCCGAGGCCGAGCAGAAATCAAAGAACTGCAAGGCAGGGCCGAGTCGAAAGCCAAGTTCAACAGGTACACACTGCTAACGGCTCCACTGGAACAGGTGTTAACCGCAATACAGGACACAAATCTGTTGAAGCGCCCGGAAAGGATGAGGTCAGATCCAGACAGGAGAAATAGAAGTAAATACTGCATGTTCCACGGGGACCACGACCACACAACCCGAGAATGCATACAATTGAGGGATGAAATAGAAACCCTAATTCGAGAAGGCTATCTCAAGGAATTCGTACAAGACAGAGGGAAGAGGCCAACACCAACAGACCAAGAGGAGGAGGCCACGGGGATACACCACCCGCACAATGACGCACTGGTAATAGTTTTAACGATCGCCAATGCCAAAGTACATCAAATTTTGGTTGATGGAGGGAGCTCAGCCGACGTCCTCTCAGCCGCCGCATTCGATGCCATGAAACTGGGAAGCGACCGCCTGAGGCCAAACCTTACGCCGTTGGTGGGATTTGGAGGAGAAAAAGTAAACCCAAGGGGAAGCATCGAGCTGCCAGTAACCTTCGGGGAAGGACAGCAGACGGTAACCAGAATGATCAACTTCCTGGTAGTGGACTGCATTCCAGCATATAATGCCATTCTGAGGCGACCAACTTTGCATGGACTTAAGGCCGTGGCCTCTACATATCATCAAGTCCTGAAATTCCCAACTGAAGACGGTGTAGGAGAGGTGCGCGGAGAACAGAAGCCCACAGCAGTCGATGCAGAGAAGACCCAAACTTATCGGGCCCACAGGCGGAAGAGGCTTAACCCACCAGACGACGGGCCGGGGTACGCTCCGGCCCCATATCCGATGATCCTCGGCCTAGGCCCAAGGGAGAGGCCGAGGGTCACGGTCGGCCTCGGCCCAAAGCCGAGGCCGACCAGGGCCAAAGGCCCGGGGCCACCTTCCGAAACGGGACGAGTTCTCAACCCTAGAAATCCCTATAAATGGGAAGGTCGCACACTCCCACAGGTCACGTTTTTCCCGCTCATCTACAAATTCACTGTTGTCTGTCAAGTGGAGCGATGGGCAAATTCCTGA

Coding sequence (CDS)

ATGGAAAGGTTGCAGCAGATGCTCCTTCCAAATGTCAGCTTCCCCTTCTTCTATGGAATCTCCTTCTCTTCCCTTCTCGATTTCTCCACTGCTGTCTCCGCCAAAGTCAATGTCGGGCATGTATGCAGAGAATGGTGGCAGGAAAAGGGCTCGAGCAGTGTCCAAGACCGAGCAGGGCCAGAAGCGATGAGAGCAGGAGCTGTCTCAGATGAGAGGTTGTTGAATTCAATCGGAGAAAGCCAACCACGGACGTATGTGGAATTCATGACTCGGGCACAGAGGTTCATAAGCGCCGAGGAACTATTGAAATCTAAACAGGAAGAAAGGGAGAGCCGGGGAGTTTCGGTGTATGACCGACATAGAGAAGACAGAGGAAAAAGACGCCGGGTGGAAGACAGCGGCCGAGAGCGACACGGACACTCCTCAGCCAATGGCCGAGGCCGAGCAGAAATCAAAGAACTGCAAGGCAGGGCCGAGTCGAAAGCCAAGTTCAACAGGTACACACTGCTAACGGCTCCACTGGAACAGGTGTTAACCGCAATACAGGACACAAATCTGTTGAAGCGCCCGGAAAGGATGAGGTCAGATCCAGACAGGAGAAATAGAAGTAAATACTGCATGTTCCACGGGGACCACGACCACACAACCCGAGAATGCATACAATTGAGGGATGAAATAGAAACCCTAATTCGAGAAGGCTATCTCAAGGAATTCGTACAAGACAGAGGGAAGAGGCCAACACCAACAGACCAAGAGGAGGAGGCCACGGGGATACACCACCCGCACAATGACGCACTGGTAATAGTTTTAACGATCGCCAATGCCAAAGTACATCAAATTTTGGTTGATGGAGGGAGCTCAGCCGACGTCCTCTCAGCCGCCGCATTCGATGCCATGAAACTGGGAAGCGACCGCCTGAGGCCAAACCTTACGCCGTTGGTGGGATTTGGAGGAGAAAAAGTAAACCCAAGGGGAAGCATCGAGCTGCCAGTAACCTTCGGGGAAGGACAGCAGACGGTAACCAGAATGATCAACTTCCTGGTAGTGGACTGCATTCCAGCATATAATGCCATTCTGAGGCGACCAACTTTGCATGGACTTAAGGCCGTGGCCTCTACATATCATCAAGTCCTGAAATTCCCAACTGAAGACGGTGTAGGAGAGGTGCGCGGAGAACAGAAGCCCACAGCAGTCGATGCAGAGAAGACCCAAACTTATCGGGCCCACAGGCGGAAGAGGCTTAACCCACCAGACGACGGGCCGGGGTACGCTCCGGCCCCATATCCGATGATCCTCGGCCTAGGCCCAAGGGAGAGGCCGAGGGTCACGGTCGGCCTCGGCCCAAAGCCGAGGCCGACCAGGGCCAAAGGCCCGGGGCCACCTTCCGAAACGGGACGAGTTCTCAACCCTAGAAATCCCTATAAATGGGAAGGTCGCACACTCCCACAGGTCACGTTTTTCCCGCTCATCTACAAATTCACTGTTGTCTGTCAAGTGGAGCGATGGGCAAATTCCTGA

Protein sequence

MERLQQMLLPNVSFPFFYGISFSSLLDFSTAVSAKVNVGHVCREWWQEKGSSSVQDRAGPEAMRAGAVSDERLLNSIGESQPRTYVEFMTRAQRFISAEELLKSKQEERESRGVSVYDRHREDRGKRRRVEDSGRERHGHSSANGRGRAEIKELQGRAESKAKFNRYTLLTAPLEQVLTAIQDTNLLKRPERMRSDPDRRNRSKYCMFHGDHDHTTRECIQLRDEIETLIREGYLKEFVQDRGKRPTPTDQEEEATGIHHPHNDALVIVLTIANAKVHQILVDGGSSADVLSAAAFDAMKLGSDRLRPNLTPLVGFGGEKVNPRGSIELPVTFGEGQQTVTRMINFLVVDCIPAYNAILRRPTLHGLKAVASTYHQVLKFPTEDGVGEVRGEQKPTAVDAEKTQTYRAHRRKRLNPPDDGPGYAPAPYPMILGLGPRERPRVTVGLGPKPRPTRAKGPGPPSETGRVLNPRNPYKWEGRTLPQVTFFPLIYKFTVVCQVERWANS

Homology

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BLAST of Spg028821 vs. NCBI nr
Match: XP_022150858.1 (uncharacterized protein LOC111018902 [Momordica charantia])

HSP 1 Score: 248.4 bits (633), Expect = 1.3e-61
Identity = 129/242 (53.31%), Postives = 165/242 (68.18%), Query Frame = 0


Query: 173 PLEQVLTAIQDTNLLKRPERMRSDPDRRNRSKYCMFHGDHDHTTRECIQLRDEIETLIRE 232
           PLEQV   I+D  LLK PERM++ P +R + +YC FH DH H T++C  L++E+E LI+ 
Sbjct: 3   PLEQVFVEIKDQRLLKWPERMKAPPSKRGKGRYCPFHLDHSHATQDCFDLKEEVEGLIQR 62

Query: 233 GYLKEFVQD----RGKR---------------PTPTD-QEEEATGIHHPHNDALVIVLTI 292
           GYLKE+V+D    + KR               PT  +  E+E T I HPHNDALVI L I
Sbjct: 63  GYLKEYVKDPKATQNKRERQAYKIYHAFVEEQPTKIEFLEDEVTHIFHPHNDALVINLKI 122

Query: 293 ANAKVHQILVDGGSSADVLSAAAFDAMKLGSDRLRPNLTPLVGFGGEKVNPRGSIELPVT 352
           ANAKVH ILVDGGSS D++S  A+  M LG   L+ +  PLVGFGGE V P G IELPVT
Sbjct: 123 ANAKVHWILVDGGSSPDIISLTAYKTMDLGERGLKSSPAPLVGFGGEWVIPEGRIELPVT 182

Query: 353 FGEGQQTVTRMINFLVVDCIPAYNAILRRPTLHGLKAVASTYHQVLKFPTEDGVGEVRGE 395
           FG G +++T+M++FLVVD   + NAIL RPT+H LKA+ S YHQ +KFPT  G+GE++GE
Sbjct: 183 FGSGPKSITKMVDFLVVDYASSCNAILGRPTMHMLKAIPSRYHQSIKFPTPGGIGEIKGE 242

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BLAST of Spg028821 vs. NCBI nr
Match: XP_022155873.1 (uncharacterized protein LOC111022886 [Momordica charantia])

HSP 1 Score: 243.0 bits (619), Expect = 5.4e-60
Identity = 126/249 (50.60%), Postives = 165/249 (66.27%), Query Frame = 0


Query: 168 TLLTAPLEQVLTAIQDTNLLKRPERMRSDPDRRNRSKYCMFHGDHDHTTRECIQLRDEIE 227
           T  T  LEQVL  I+D  LLK PERM++ P +R++ +Y +FH DH H T++C  L++E+E
Sbjct: 6   TPTTVSLEQVLMEIKDQRLLKWPERMKAPPSKRSKGQYWLFHRDHGHATQDCFDLKEEVE 65

Query: 228 TLIREGYLKEFVQD---------------RGKRPTPTDQ-------EEEATGIHHPHNDA 287
            LIR GYLKE+V+D               R  R    +Q       E+EAT + HPHNDA
Sbjct: 66  GLIRSGYLKEYVEDPKATQNGENDSNSPVREIRTIMEEQPAKLEFSEDEATHLFHPHNDA 125

Query: 288 LVIVLTIANAKVHQILVDGGSSADVLSAAAFDAMKLGSDRLRPNLTPLVGFGGEKVNPRG 347
           LVI L IANAKVH+ILVDGGSS  ++S  A+ AM LG   L+ +  PLVGFGGE+V P G
Sbjct: 126 LVITLKIANAKVHRILVDGGSSTYIISLTAYKAMDLGEKALKSSPVPLVGFGGERVIPEG 185

Query: 348 SIELPVTFGEGQQTVTRMINFLVVDCIPAYNAILRRPTLHGLKAVASTYHQVLKFPTEDG 395
            IELPV FG G +++ +M++FLVV    +YN IL RP +H LK + STYHQ +KFPT  G
Sbjct: 186 RIELPVMFGSGPKSIIKMVDFLVVYYASSYNVILGRPKMHMLKEIPSTYHQSMKFPTSSG 245

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BLAST of Spg028821 vs. NCBI nr
Match: XP_022156748.1 (uncharacterized protein LOC111023587 [Momordica charantia])

HSP 1 Score: 241.5 bits (615), Expect = 1.6e-59
Identity = 129/254 (50.79%), Postives = 165/254 (64.96%), Query Frame = 0


Query: 167 YTLLTAPLEQVLTAIQDTNLLKRPERMRSDPDRRNRSKYCMFHGDHDHTTRECIQLRDEI 226
           YTL T PLEQVL  I+D  LLK PERM++   +R++ +YC+FH DHDH T++C  L++E+
Sbjct: 2   YTLTTIPLEQVLMEIKDQRLLKWPERMKAPSTKRSKGRYCLFHRDHDHATQDCFDLKEEV 61

Query: 227 ETLIREGYLKE--------------------------FVQDRGKRPTPTDQEEEATGIHH 286
           E LIR GYLKE                          ++ DR    T    E+EAT + H
Sbjct: 62  EGLIRRGYLKEERIWEKKKADVREARANREQNEVYHAYITDR--PVTIEFSEDEATQLFH 121

Query: 287 PHNDALVIVLTIANAKVHQILVDGGSSADVLSAAAFDAMKLGSDRLRPNLTPLVGFGGEK 346
            HNDALVI L IAN KVH+ILVDGGSSAD++S  A+ AM L    L+ +  PLVGFGGE+
Sbjct: 122 SHNDALVITLKIANVKVHRILVDGGSSADIISWTAYKAMDLEEKVLKSSPAPLVGFGGER 181

Query: 347 VNPRGSIELPVTFGEGQQTVTRMINFLVVDCIPAYNAILRRPTLHGLKAVASTYHQVLKF 395
           V   G IELPVTFG G + VT+M++FLVV+   +YN IL R T+H LK + STYHQ +KF
Sbjct: 182 VISEGRIELPVTFGSGPKGVTKMVDFLVVNYTSSYNEILGRLTMHMLKVIPSTYHQSMKF 241

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BLAST of Spg028821 vs. NCBI nr
Match: XP_030936700.1 (uncharacterized protein LOC115961955 [Quercus lobata])

HSP 1 Score: 239.2 bits (609), Expect = 7.8e-59
Identity = 153/388 (39.43%), Postives = 206/388 (53.09%), Query Frame = 0


Query: 68  VSDERLLNSIGESQPRTYVEFMTRAQRFISAEELLKSKQEERESRGVSVYDRHREDRGKR 127
           V  +  ++ + E +P+T  E +  AQ F++AE+ + +K                    KR
Sbjct: 14  VHSDLFIHKLYEQEPQTMAELVHSAQNFMNAEDAIIAK--------------------KR 73

Query: 128 RRVE--DSGRERHGHSSAN-GRGRAEIKELQGR-AESKAKFNRYTLLTAPLEQVLTAIQD 187
           +++E  D+   RH     +  +GR E K+ + R A    +  +YT L  PL+QVL  I+D
Sbjct: 74  KKIEKMDANPTRHSEQGPHLKKGRTEDKKDRDRKAGPSVRSQQYTPLNMPLDQVLMQIKD 133

Query: 188 TNLLKRPERMRSDPDRRNRSKYCMFHGDHDHTTRECIQLRDEIETLIREGYLKEF----- 247
              LK PE+M+ DP++RNR+KYC FH DH H T EC  L+ +IE LIR+G L+ F     
Sbjct: 134 DPSLKWPEKMKGDPNKRNRNKYCRFHRDHGHDTDECFDLKQQIENLIRQGKLRSFLGRDH 193

Query: 248 ---------------------------------------------VQDRGKRPTPTD--- 307
                                                        VQ  G+ P   D   
Sbjct: 194 KDEKLKGKAEESSRPPLGEIRVIVGGSSTVQSSRSRKTYLKVVQSVQLSGRPPRDRDTDE 253

Query: 308 -----QEEEATGIHHPHNDALVIVLTIANAKVHQILVDGGSSADVLSAAAFDAMKLGSDR 367
                 EEEA  IHHPH+DA+VI L IA+    ++LVD GSSAD+L   AF  MKLG DR
Sbjct: 254 QAITFTEEEAKRIHHPHDDAIVITLLIADYTTRRVLVDNGSSADILYLPAFQQMKLGRDR 313

Query: 368 LRPNLTPLVGFGGEKVNPRGSIELPVTFGEGQQTVTRMINFLVVDCIPAYNAILRRPTLH 394
           LRP  +PLVGFGG KV P G + LPV  G   Q VT+ ++FLVVDC  +YNAI+ RPTL+
Sbjct: 314 LRPVNSPLVGFGGMKVQPVGIVTLPVVVGAYPQQVTKDVSFLVVDCSSSYNAIIGRPTLN 373

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BLAST of Spg028821 vs. NCBI nr
Match: XP_030958631.1 (uncharacterized protein LOC115980538 [Quercus lobata])

HSP 1 Score: 238.0 bits (606), Expect = 1.7e-58
Identity = 157/420 (37.38%), Postives = 221/420 (52.62%), Query Frame = 0


Query: 68  VSDERLLNSIGESQPRTYVEFMTRAQRFISAEELLKSKQEERESRGVSVYDRHRED--RG 127
           ++ +  ++ + E +P+T  E +  AQ F++AE+ + +K+ +R  R  +   RH E   R 
Sbjct: 234 INSDLFIHKLYEKEPQTMAELVHSAQNFMNAEDAIIAKKRKRAERMEAHPARHSEQAPRP 293

Query: 128 KRRRVEDSGRERHGHSSANGRGRAEIKELQGRAESKAKFNRYTLLTAPLEQVLTAIQDTN 187
           K+ R ED  +ER G  +             GR+++      YT L APL QVL  I+D  
Sbjct: 294 KKGRTEDR-KERDGRKTGP----------LGRSQN------YTPLNAPLNQVLMQIKDDP 353

Query: 188 LLKRPERMRSDPDRRNRSKYCMFHGDHDHTTRECIQLRDEIETLIREGYLKEFV------ 247
            LK PE+M+ DP++RN++KYC FH DH H T EC  L+ +IE LIR+G LK FV      
Sbjct: 354 SLKWPEKMKGDPNKRNKNKYCRFHRDHGHDTDECYDLKQQIENLIRQGKLKHFVGRDRTD 413

Query: 248 -QDRGK---------------------------------------------------RPT 307
            + +GK                                                    PT
Sbjct: 414 EKQKGKMEESSRPPLGEIRIIVGGNPMGQSSKSKKTYLKAVQNVQLSGRTPRTRPMDEPT 473

Query: 308 PTDQEEEATGIHHPHNDALVIVLTIANAKVHQILVDGGSSADVLSAAAFDAMKLGSDRLR 367
            +   E+A  IHHPH+DA+VI L IA+    ++LVD GSSADVL   AF  M+LG D+LR
Sbjct: 474 ISFTNEDAERIHHPHDDAIVITLLIADYTTRRVLVDNGSSADVLYYPAFQQMRLGRDQLR 533

Query: 368 PNLTPLVGFGGEKVNPRGSIELPVTFGEGQQTVTRMINFLVVDCIPAYNAILRRPTLHGL 420
              +PL+GFGG KV P G+I LPV  G   Q +T+ +NFLVVDC  +YNAI+ RPTL+  
Sbjct: 534 AVCSPLIGFGGMKVQPVGTITLPVVVGSYPQQITKEVNFLVVDCTSSYNAIIGRPTLNSW 593

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BLAST of Spg028821 vs. ExPASy TrEMBL
Match: A0A6J1DAK4 (uncharacterized protein LOC111018902 OS=Momordica charantia OX=3673 GN=LOC111018902 PE=4 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 6.2e-62
Identity = 129/242 (53.31%), Postives = 165/242 (68.18%), Query Frame = 0


Query: 173 PLEQVLTAIQDTNLLKRPERMRSDPDRRNRSKYCMFHGDHDHTTRECIQLRDEIETLIRE 232
           PLEQV   I+D  LLK PERM++ P +R + +YC FH DH H T++C  L++E+E LI+ 
Sbjct: 3   PLEQVFVEIKDQRLLKWPERMKAPPSKRGKGRYCPFHLDHSHATQDCFDLKEEVEGLIQR 62

Query: 233 GYLKEFVQD----RGKR---------------PTPTD-QEEEATGIHHPHNDALVIVLTI 292
           GYLKE+V+D    + KR               PT  +  E+E T I HPHNDALVI L I
Sbjct: 63  GYLKEYVKDPKATQNKRERQAYKIYHAFVEEQPTKIEFLEDEVTHIFHPHNDALVINLKI 122

Query: 293 ANAKVHQILVDGGSSADVLSAAAFDAMKLGSDRLRPNLTPLVGFGGEKVNPRGSIELPVT 352
           ANAKVH ILVDGGSS D++S  A+  M LG   L+ +  PLVGFGGE V P G IELPVT
Sbjct: 123 ANAKVHWILVDGGSSPDIISLTAYKTMDLGERGLKSSPAPLVGFGGEWVIPEGRIELPVT 182

Query: 353 FGEGQQTVTRMINFLVVDCIPAYNAILRRPTLHGLKAVASTYHQVLKFPTEDGVGEVRGE 395
           FG G +++T+M++FLVVD   + NAIL RPT+H LKA+ S YHQ +KFPT  G+GE++GE
Sbjct: 183 FGSGPKSITKMVDFLVVDYASSCNAILGRPTMHMLKAIPSRYHQSIKFPTPGGIGEIKGE 242

[Close]

BLAST of Spg028821 vs. ExPASy TrEMBL
Match: A0A6J1DP33 (uncharacterized protein LOC111022886 OS=Momordica charantia OX=3673 GN=LOC111022886 PE=4 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 2.6e-60
Identity = 126/249 (50.60%), Postives = 165/249 (66.27%), Query Frame = 0


Query: 168 TLLTAPLEQVLTAIQDTNLLKRPERMRSDPDRRNRSKYCMFHGDHDHTTRECIQLRDEIE 227
           T  T  LEQVL  I+D  LLK PERM++ P +R++ +Y +FH DH H T++C  L++E+E
Sbjct: 6   TPTTVSLEQVLMEIKDQRLLKWPERMKAPPSKRSKGQYWLFHRDHGHATQDCFDLKEEVE 65

Query: 228 TLIREGYLKEFVQD---------------RGKRPTPTDQ-------EEEATGIHHPHNDA 287
            LIR GYLKE+V+D               R  R    +Q       E+EAT + HPHNDA
Sbjct: 66  GLIRSGYLKEYVEDPKATQNGENDSNSPVREIRTIMEEQPAKLEFSEDEATHLFHPHNDA 125

Query: 288 LVIVLTIANAKVHQILVDGGSSADVLSAAAFDAMKLGSDRLRPNLTPLVGFGGEKVNPRG 347
           LVI L IANAKVH+ILVDGGSS  ++S  A+ AM LG   L+ +  PLVGFGGE+V P G
Sbjct: 126 LVITLKIANAKVHRILVDGGSSTYIISLTAYKAMDLGEKALKSSPVPLVGFGGERVIPEG 185

Query: 348 SIELPVTFGEGQQTVTRMINFLVVDCIPAYNAILRRPTLHGLKAVASTYHQVLKFPTEDG 395
            IELPV FG G +++ +M++FLVV    +YN IL RP +H LK + STYHQ +KFPT  G
Sbjct: 186 RIELPVMFGSGPKSIIKMVDFLVVYYASSYNVILGRPKMHMLKEIPSTYHQSMKFPTSSG 245

[Close]

BLAST of Spg028821 vs. ExPASy TrEMBL
Match: A0A6J1DRG9 (uncharacterized protein LOC111023587 OS=Momordica charantia OX=3673 GN=LOC111023587 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 7.6e-60
Identity = 129/254 (50.79%), Postives = 165/254 (64.96%), Query Frame = 0


Query: 167 YTLLTAPLEQVLTAIQDTNLLKRPERMRSDPDRRNRSKYCMFHGDHDHTTRECIQLRDEI 226
           YTL T PLEQVL  I+D  LLK PERM++   +R++ +YC+FH DHDH T++C  L++E+
Sbjct: 2   YTLTTIPLEQVLMEIKDQRLLKWPERMKAPSTKRSKGRYCLFHRDHDHATQDCFDLKEEV 61

Query: 227 ETLIREGYLKE--------------------------FVQDRGKRPTPTDQEEEATGIHH 286
           E LIR GYLKE                          ++ DR    T    E+EAT + H
Sbjct: 62  EGLIRRGYLKEERIWEKKKADVREARANREQNEVYHAYITDR--PVTIEFSEDEATQLFH 121

Query: 287 PHNDALVIVLTIANAKVHQILVDGGSSADVLSAAAFDAMKLGSDRLRPNLTPLVGFGGEK 346
            HNDALVI L IAN KVH+ILVDGGSSAD++S  A+ AM L    L+ +  PLVGFGGE+
Sbjct: 122 SHNDALVITLKIANVKVHRILVDGGSSADIISWTAYKAMDLEEKVLKSSPAPLVGFGGER 181

Query: 347 VNPRGSIELPVTFGEGQQTVTRMINFLVVDCIPAYNAILRRPTLHGLKAVASTYHQVLKF 395
           V   G IELPVTFG G + VT+M++FLVV+   +YN IL R T+H LK + STYHQ +KF
Sbjct: 182 VISEGRIELPVTFGSGPKGVTKMVDFLVVNYTSSYNEILGRLTMHMLKVIPSTYHQSMKF 241

[Close]

BLAST of Spg028821 vs. ExPASy TrEMBL
Match: A0A2N9EYR7 (Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS7874 PE=4 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.3e-54
Identity = 139/353 (39.38%), Postives = 190/353 (53.82%), Query Frame = 0


Query: 74  LNSIGESQPRTYVEFMTRAQRFISAEELL---------KSKQEERESRGVSVYDRHREDR 133
           L S+ +  P T  E M  AQR ++ EE L         K K E  +    S   R +  R
Sbjct: 109 LFSVYKDPPSTMTEMMYEAQRHMNGEEALLARDQTIGKKRKWEHPDRPAESHETRPKAQR 168

Query: 134 GKRRRVEDSGRERHGHSSANGRGRAEIKELQGRAESKAKFNRYTLLTAPLEQVLTAIQDT 193
            + RR ED           +GRG  E            +FN +T L AP++ +   I++ 
Sbjct: 169 NRNRRQED----------RSGRGFNE------------RFNHFTPLNAPVDHIFMQIRND 228

Query: 194 NLLKRPERMRSDPDRRNRSKYCMFHGDHDHTTRECIQLRDEIETLIREGYLKEFVQ--DR 253
             LK P ++ +DPD+R R KYC FH DH H T +C  L+ +IE LI++G L+ F++   R
Sbjct: 229 PALKWPGKLLTDPDKRPRDKYCRFHRDHGHNTEDCYDLKRQIEELIKQGKLQRFIERGQR 288

Query: 254 GKRPTPTDQ----------------------EEEATGIHHPHNDALVIVLTIANAKVHQI 313
             RP  T Q                      EE+A  + HPH+DALVI L IA     ++
Sbjct: 289 EGRPQGTRQQRPPVEKMDKKPRLEDIPITFGEEDARKLFHPHDDALVITLEIAGYSTRRV 348

Query: 314 LVDGGSSADVLSAAAFDAMKLGSDRLRPNLTPLVGFGGEKVNPRGSIELPVTFGEGQQTV 373
           L+D GSSAD++   AF  M++  D+LRP  TPLVGF G  V P G I L +  G   +  
Sbjct: 349 LIDNGSSADIIYLTAFQQMRIDKDQLRPIETPLVGFAGTSVYPLGIITLQIIAGTYPKQA 408

Query: 374 TRMINFLVVDCIPAYNAILRRPTLHGLKAVASTYHQVLKFPTEDGVGEVRGEQ 394
           TR +NFLVVDC  AYN I+ RPTL+ L+AV STYH +++FPTE+G+GE+RG+Q
Sbjct: 409 TRKVNFLVVDCPSAYNVIIGRPTLNRLRAVTSTYHLLVRFPTENGIGEMRGDQ 439

[Close]

BLAST of Spg028821 vs. ExPASy TrEMBL
Match: A0A2N9H457 (RNase H domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS34293 PE=4 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.3e-54
Identity = 139/353 (39.38%), Postives = 190/353 (53.82%), Query Frame = 0


Query: 74  LNSIGESQPRTYVEFMTRAQRFISAEELL---------KSKQEERESRGVSVYDRHREDR 133
           L S+ +  P T  E M  AQR ++ EE L         K K E  +    S   R +  R
Sbjct: 329 LFSVYKDPPSTMTEMMYEAQRHMNGEEALLARDQTIGKKRKWEHPDRPAESHETRPKAQR 388

Query: 134 GKRRRVEDSGRERHGHSSANGRGRAEIKELQGRAESKAKFNRYTLLTAPLEQVLTAIQDT 193
            + RR ED           +GRG  E            +FN +T L AP++ +   I++ 
Sbjct: 389 NRNRRQED----------RSGRGFNE------------RFNHFTPLNAPVDHIFMQIRND 448

Query: 194 NLLKRPERMRSDPDRRNRSKYCMFHGDHDHTTRECIQLRDEIETLIREGYLKEFVQ--DR 253
             LK P ++ +DPD+R R KYC FH DH H T +C  L+ +IE LI++G L+ F++   R
Sbjct: 449 PALKWPGKLLTDPDKRPRDKYCRFHRDHGHNTEDCYDLKRQIEELIKQGKLQRFIERGQR 508

Query: 254 GKRPTPTDQ----------------------EEEATGIHHPHNDALVIVLTIANAKVHQI 313
             RP  T Q                      EE+A  + HPH+DALVI L IA     ++
Sbjct: 509 EGRPQGTRQQRPPVEKMDKKPRLEDIPITFGEEDARKLFHPHDDALVITLEIAGYSTRRV 568

Query: 314 LVDGGSSADVLSAAAFDAMKLGSDRLRPNLTPLVGFGGEKVNPRGSIELPVTFGEGQQTV 373
           L+D GSSAD++   AF  M++  D+LRP  TPLVGF G  V P G I L +  G   +  
Sbjct: 569 LIDNGSSADIIYLTAFQQMRIDKDQLRPIETPLVGFAGTSVYPLGIITLQIIAGTYPKQA 628

Query: 374 TRMINFLVVDCIPAYNAILRRPTLHGLKAVASTYHQVLKFPTEDGVGEVRGEQ 394
           TR +NFLVVDC  AYN I+ RPTL+ L+AV STYH +++FPTE+G+GE+RG+Q
Sbjct: 629 TRKVNFLVVDCPSAYNVIIGRPTLNRLRAVTSTYHLLVRFPTENGIGEMRGDQ 659

The following BLAST results are available for this feature:

BLAST of Spg028821 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150858.1	1.3e-61	53.31	uncharacterized protein LOC111018902 [Momordica charantia]	[more]
XP_022155873.1	5.4e-60	50.60	uncharacterized protein LOC111022886 [Momordica charantia]	[more]
XP_022156748.1	1.6e-59	50.79	uncharacterized protein LOC111023587 [Momordica charantia]	[more]
XP_030936700.1	7.8e-59	39.43	uncharacterized protein LOC115961955 [Quercus lobata]	[more]
XP_030958631.1	1.7e-58	37.38	uncharacterized protein LOC115980538 [Quercus lobata]	[more]

BLAST of Spg028821 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg028821 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DAK4	6.2e-62	53.31	uncharacterized protein LOC111018902 OS=Momordica charantia OX=3673 GN=LOC111018...	[more]
A0A6J1DP33	2.6e-60	50.60	uncharacterized protein LOC111022886 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1DRG9	7.6e-60	50.79	uncharacterized protein LOC111023587 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A2N9EYR7	1.3e-54	39.38	Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS7874 PE=4 SV=1	[more]
A0A2N9H457	1.3e-54	39.38	RNase H domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS34293 ...	[more]

BLAST of Spg028821 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 212..232
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 386..417
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 386..471
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 107..150
None	No IPR available	PANTHER	PTHR33240:SF7	GAG-PRO-LIKE PROTEIN	coord: 158..244
None	No IPR available	PANTHER	PTHR33240	OS08G0508500 PROTEIN	coord: 267..393 coord: 158..244
None	No IPR available	PANTHER	PTHR33240:SF7	GAG-PRO-LIKE PROTEIN	coord: 267..393
None	No IPR available	CDD	cd00303	retropepsin_like	coord: 268..365 e-value: 1.78788E-11 score: 58.502
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 251..386 e-value: 3.5E-7 score: 31.9

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg028821.1	Spg028821.1	mRNA