Spg028742 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg028742
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationscaffold7: 13081472 .. 13087053 (-)
RNA-Seq ExpressionSpg028742
SyntenySpg028742
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTTCGCAATGCACACCCTCTGCAACTCAAATCCCCTCTCAATATTTCAACCCACCTTCCCTTCCAAAAACCCTAATCAGACTTCGAAAAGGAAATTCCACATCGTAAAAATGGCCGCCGATTTCGATCGAGCCACCGAATTGAAGCAATTCGACCAAACCAAAGCCGGCGTCAAAGGCCTTCTCGACGCCGGCCTCAAACAAATCCCTCGCATCTTCATCCACCCGCCGGAAACTCTCTCCGATCTCAAACCAACCCTTAAATCCCCATCCTCCGCCGCCGTCGTCGTTCCGACCATCGACCTCGCCGGCATCCACTCCCCCGACCTCCGCCCCTCAATCGTCGACCAGATCCGGAACGCCGCGTCGACCTTCGGATTCTTCCAGATAATCAACCATGGAGTTCCGCAAAAAGTGCTCGACGAAATCCTCGATTCGGTCAGGGCTTTTCACGAGCTGCCGTCGGAGGCGAAGTCGCCGTACTACCGGAGGGACGTTGGGACCGGAGTGTCGTACTTGTCGAACATCGATCTGTTCAACGCGAAGGCGGCGAGTTGGAGGGACACGATTCAGATAGGGCTGGGGCCGGTGGCGGCGGCGACGGAGAATATTCCGGAGACCTGTAGGGCGGCGGTGGCGGAGTGGGCGGTGGAGACGGAGAGGTTGGGGTATTTGTTGGAGGAGGTGCTGTGTGAGGGATTAGGGCTGAAATCGGAGCGGCTGAAGGAGGCGACGTACGGAGAAGGGCGAATGATGGTGGGGCATTACTACCCGTACTGCCCGCAGCCGGATCTGACGGTGGGGATTAAATCGCACACGGATCCTGGAGTTTTGACGGTGTTGCTGCAGGACCATATCGGCGGTTTGCAGATCCGGCACGGCGGTGGGTGGGTGGATGTTGAGCCTGTCGCCGGTGCTCTAGTAATCAATATCGGAGACTTGCTTCAGGTAACTTTTATACAGACCGAAAAATTATTAGGTGCCGTGCAATGCGATATAATAATTCTTTTAATTTATTTATAATTAGCAATGGATTATCCAATTTTGAAAATTTAAAACACTTTTGAAATTTTTTAAAATTTGGTGTCCTACAAACTGAAAGTTTATTGGGATTCTCGTGCACATCTATTTGAGACAAATCTAAAAGTTTGATACTAAAATAGTAATTTTGGTCTTGGACTTTTCAGATAAAATAGATTCTTGAACTTTAGAATATACTTTTTAGAATTCAAAGATATTAAACATGAATTGCTAATATTCTTTTTTTTTAAAAAAAAAAAAAACAAACGATTAGAAGATGTAATTTAAAGTCCATTTGGAATACATTTTAAGTTAAATTTTTTTCAAGAATGCATTTTTTAAAAAACATTTTGTATACAAATTGTTTAGAATTTAAACACTCATATGTTTGGTTGCATTTTCTCATAAGTGTTTTTATAATACTTAAAGACTCTTTTTTAAAAAGTTGGTTTCAAGTGTTTCTCCCAAAATTGTTTATTTATCAATCTCATTTTCTAATAAGTCATTTTTAGTGGTTGTCAAACATTTTGTATACATTTTTTTCAAAATCAAAATTAAACACTTGAAAAGTTAAACCGAACACACCCTTAACCATAACCATGAGAAATTGTTATGAAACTTTTTTTTTAGGATAAAATGCGACTATTTTTCCAATTTAAAAAACCTTCATGAACTTGGAGGCGCTTGTGAAATATTCTCCAATTTTGAAAATTTAAACTTAATTTGATAACGTTTTAGAAGTTATTTTTGAAATCTTAACTAATTTGAAAAATAATACGGTAGGTTTTGAGTTTGAACATTTTTTCATTTTAAAATTTAACTAACATTTTGAAAGATTGATTACACTATAATATTATAAATATTAAATTAATTAAAATAAAACTATCATGGGTAAGTTTAAAAATATTTATAAAAAGTAGATAACAAATGAATGTAATTAGCAAAAGCGAGCTAGTTCAGTGTTAATTGACATGTATTTTGAAGTTCGAATTCCTCACCCCCAATTGAACTCAAAAAAAAAAAAAAAAACACTAAAAATCCAACGATATTAAATGGACCTAAAATTTTCCAAATGTTGCACTTTGATCTTGATTTTGGTCCATTTTTGTATATACATACAGAGATAAAATTACATATTTAATTTATTGTTTTAAATTTGAATTCATTAAATATTCATCTTAAATTATTAAGTTTTTTTTTGTAAGTTCAATGAAAATGGGCTTTTCAAAAAAAAAAAAACCCACATTGAAAATGGAAGATTCAAACTTTTAACCTCTTGATCGAAAATGTAGATCAATTATCACAAATTTATGCTTCCTTTCATAAATTATTTTTAAATCTATACAAATCTCTCGAACGATGTGCAGAACAATTCAAATATTAAAAAAGAAAAGTGGTAGAAAAAAGGCTGACTTTTCCTTAAAAAATAATCAAACCAAGGACAGACAAACCTTCCAAAGAAATCTATCACGTCTAGATTCTCAAATATTATTTTATTTAATTCAGTTTCCTAATTTTTAGTAAATTATACTTTTGTCTAGGATCTAAATTTTAGCCTCGGTTTAGTAAAGCGATTTGGTTTTTTTTTTTTTTTTACTTTTGTAAATTAAACTTATAAAAACTACTTCCACGAAGTCAAGTTTTGAAAATTACTTTAGTTTTCTAAAAACATGTTTTTGTTTTTGAAACTTGATTAAGAATTCAAACGTGTCTTCGACAAAAATGGAACCATGGTAAGAAATTTGAAAGAAAACAAGCATAAACAAAACCTTAAAAAATGAAAAACAAAAATTATTTCAAAAATAAAAACAAAAGACAAAAAACCAAGTGATTATCAAATGAGGCCTTAGTAGTTAGTAGGAATGTTTACGGGACAAGGCAAGGATGAGATGACTTTCCCGTCCTCGTCCACTTCCCCGTCCTTGCCTCCCATTTCAATCTCCATCCCCATGAAATTTCCCGTCAAATTTCACGGGAATGGAGGAGGGATTCCCTACAGAGAATTTGCGGGGATTGGACTCGCCATGGAGAATTTCCCCATGTATCTTTTTTCTTCTTTTTTGATCTCAATTGAATCAAATAATTATTTTGACAAAATTTCAAATAGTTAACTTTTCATGGGAAATGTTCATTAATTTTCAATTAGATAGTTAATTATTCACACACGAAATATCAAATTATCTATACATATAATTCAAACATATCCAATTTTCTTAAGTAATTAACAATAAAACTTTAAATTTATACATAAAGAGAAATAATAATAATAATAAATTAAATACAGTGGAAAAAGGAGAATGAGGATGGAGAATACATCCACGTCCTCGTTTAGCTCACGGGGAATTTTTTTCCTCCACTCCCTCCCCCATTCCCCGCCCCATTAGAACAGGTCCCCACGGAGCCTTGTCCTCGCAGGACAAATGGACATCTCTAAGCCTTAGTTCATATATTGCCTATAAGTTTTAGAAAGTAATTCTAAATGGTCATGAAATTTGAACTTGATTGACCTGAAAAAATATACCAAAAAAATCAATTTATATTCTAAATTTTAGGGGTTTTATCACTTAAAACTCCAAGCTAATAATTGTATCAATTTTAATCCTAAATTTTTATAAATGAATTAGTTTAGATAGGATTATCCCATTTTAAAACCATTAATACATCTCTTTCAAAAAAAAAAAAAAAAACCATTAATACATAAATTTCACATGTGTATAAGGTTTCTTTAAATGCATTGATTAACGTAATATAATATTAGAGAGAGATGCAAGAATCAAATGATAGTAATGGCTTTTCATGATTAATATGAGTTGTTCTTCATCTTTTTGGGTTCCTCTATTTTTCGCTACTTAATTAAGATGAATTATGTGATAGCTAGATAAATTACATGATAATTTTTCTTGCTACTTTGGTATGATTTTAGTCGAAGAATGATTTTATTTATGAGTTGATTTTAAATGGAATCAAGTGAAAATAGTAATAATTCACTCACAGAAGGTCTACATTGATACAATTATTAGTTGAGATTTTAAATGATATAACCCCCAAAGTTTAGGGGTATAAATTATTTTTTCTTCAACATATATTACTTTTATATCTTAATTTAGTGATTAGACAATGTCGTGATATCTCTTCCTTTTTTTTTTTTTTTTCTCTTTTTCTGCTAGATTGACAAAATCTATTAATTTCATATCAAATAAACACATTTTACCGAGTCAGTGTTGTCCATCTAACAAGCACCTACCACATCATCATTCCATTAGGTTAACCAACGGTGGGTAAGTTTAGGGACCATTTTAAGGGAAAATTTTCTAGAACTACGAGAGTACCTACTCCCATATGTTCACATTATGACTTTCCCATTATAAACAATTCTTTAATGCAACTCGACTCTCCACTTTTCTAAATGATGTTGACGTAGCTGACAATTAATGAGAGTAATATCTATATTTTTTATATAGATCGCATCAAATGATGTGTACAAGAGCGTGGAGCACAGAGTATTGGCGAATGGGAAGTCAGAAGCGCGGGTTTCGGTTGCGGTTTTCTACAACCCTCGAATCAGAGACAGCTTGTACGGACCACTCCCAGAGCTAATCTCGCAGGACACACCTGCAGTTTATCGCCACTTCACTTATGATGATTACATAACAAGGTTCTTCAACAAGGAGTTGGATGGCAAATCTTTGCCCAACCATTATAGAATTTGAACACTCTCTCAACTCTTCCTCTTTCCTATATAAGTATATATCATATATTTTGAATATGCAAACAACTTTTGCAAACAATTTTTTCCCCTTTTTCATGTTTAGGTTCATATATTTGTGATAGATTGGTAGGTTGGTGGATTAGTTTTTGTTTGTTCTACTTTGACCTTCTATGAGATGGAGTGGTGATCAATCTCTGTATGCTTGGAGCGATTATATGATGCATAGGATTAGTTGCTAAGTATGTGGTAATAATATTATCACATAAGAGCAAAGGAACAATTAGAGAAGAAACATCAATTTGGCAGTGGCACTGGACATGCCATGCCATGCCTTTATACTTGGATCCGACATTCAAACGAGACACAATTTTTTGCGTAGTTGAGGACCAAGAAATCAAGTTATTACCAAGAAACACACAATATCCACTAGTACTCCTTTAGTCATCGGGCAATTGGCCCAATTGGTATCGTTGTAGCATGTCAAAGAGTGATGGTCTGATTTTGTAAAAGTAATACTAAGATTGCTGGTTGTCTTCAAATACCCAAGTATGTATTTCAATGAGTTCCATGGATTGTAGCAGGGGCATGGAGAAATTAACCTACCTTATTAATGACGAAAAAACTAATCAGACTGAGTGAGCATACAATACACTACAAGAAAATGAGCTTTCGTCGATGCCAAAAATGGGTCGAGAAAAGCAAAAAACGTGTTGGAAAAGATTCTTCCGGACAAAGGCGTTGAAACAAATCTGTCAAGTTCTGTCAACGCCACCTCATTGGTGTTGGCAGAAGATAGAAGAAGAGACTGCTGCCAATTAGCACCGACAAAAGAGAGGAGTCGAGAGAAGTAGC

mRNA sequence

ATGCACACCCTCTGCAACTCAAATCCCCTCTCAATATTTCAACCCACCTTCCCTTCCAAAAACCCTAATCAGACTTCGAAAAGGAAATTCCACATCGTAAAAATGGCCGCCGATTTCGATCGAGCCACCGAATTGAAGCAATTCGACCAAACCAAAGCCGGCGTCAAAGGCCTTCTCGACGCCGGCCTCAAACAAATCCCTCGCATCTTCATCCACCCGCCGGAAACTCTCTCCGATCTCAAACCAACCCTTAAATCCCCATCCTCCGCCGCCGTCGTCGTTCCGACCATCGACCTCGCCGGCATCCACTCCCCCGACCTCCGCCCCTCAATCGTCGACCAGATCCGGAACGCCGCGTCGACCTTCGGATTCTTCCAGATAATCAACCATGGAGTTCCGCAAAAAGTGCTCGACGAAATCCTCGATTCGGTCAGGGCTTTTCACGAGCTGCCGTCGGAGGCGAAGTCGCCGTACTACCGGAGGGACGTTGGGACCGGAGTGTCGTACTTGTCGAACATCGATCTGTTCAACGCGAAGGCGGCGAGTTGGAGGGACACGATTCAGATAGGGCTGGGGCCGGTGGCGGCGGCGACGGAGAATATTCCGGAGACCTGTAGGGCGGCGGTGGCGGAGTGGGCGGTGGAGACGGAGAGGTTGGGGTATTTGTTGGAGGAGGTGCTGTGTGAGGGATTAGGGCTGAAATCGGAGCGGCTGAAGGAGGCGACGTACGGAGAAGGGCGAATGATGGTGGGGCATTACTACCCGTACTGCCCGCAGCCGGATCTGACGGTGGGGATTAAATCGCACACGGATCCTGGAGTTTTGACGGTGTTGCTGCAGGACCATATCGGCGGTTTGCAGATCCGGCACGGCGGTGGGTGGGTGGATGTTGAGCCTGTCGCCGGTGCTCTAGTAATCAATATCGGAGACTTGCTTCAGATCGCATCAAATGATGTGTACAAGAGCGTGGAGCACAGAGTATTGGCGAATGGGAAGTCAGAAGCGCGGGTTTCGGTTGCGGTTTTCTACAACCCTCGAATCAGAGACAGCTTGTACGGACCACTCCCAGAGCTAATCTCGCAGGACACACCTGCAGTTTATCGCCACTTCACTTATGATGATTACATAACAAGGTTCTTCAACAAGGAGTTGGATGGCAAATCTTTGCCCAACCATTATAGAATTTGA

Coding sequence (CDS)

ATGCACACCCTCTGCAACTCAAATCCCCTCTCAATATTTCAACCCACCTTCCCTTCCAAAAACCCTAATCAGACTTCGAAAAGGAAATTCCACATCGTAAAAATGGCCGCCGATTTCGATCGAGCCACCGAATTGAAGCAATTCGACCAAACCAAAGCCGGCGTCAAAGGCCTTCTCGACGCCGGCCTCAAACAAATCCCTCGCATCTTCATCCACCCGCCGGAAACTCTCTCCGATCTCAAACCAACCCTTAAATCCCCATCCTCCGCCGCCGTCGTCGTTCCGACCATCGACCTCGCCGGCATCCACTCCCCCGACCTCCGCCCCTCAATCGTCGACCAGATCCGGAACGCCGCGTCGACCTTCGGATTCTTCCAGATAATCAACCATGGAGTTCCGCAAAAAGTGCTCGACGAAATCCTCGATTCGGTCAGGGCTTTTCACGAGCTGCCGTCGGAGGCGAAGTCGCCGTACTACCGGAGGGACGTTGGGACCGGAGTGTCGTACTTGTCGAACATCGATCTGTTCAACGCGAAGGCGGCGAGTTGGAGGGACACGATTCAGATAGGGCTGGGGCCGGTGGCGGCGGCGACGGAGAATATTCCGGAGACCTGTAGGGCGGCGGTGGCGGAGTGGGCGGTGGAGACGGAGAGGTTGGGGTATTTGTTGGAGGAGGTGCTGTGTGAGGGATTAGGGCTGAAATCGGAGCGGCTGAAGGAGGCGACGTACGGAGAAGGGCGAATGATGGTGGGGCATTACTACCCGTACTGCCCGCAGCCGGATCTGACGGTGGGGATTAAATCGCACACGGATCCTGGAGTTTTGACGGTGTTGCTGCAGGACCATATCGGCGGTTTGCAGATCCGGCACGGCGGTGGGTGGGTGGATGTTGAGCCTGTCGCCGGTGCTCTAGTAATCAATATCGGAGACTTGCTTCAGATCGCATCAAATGATGTGTACAAGAGCGTGGAGCACAGAGTATTGGCGAATGGGAAGTCAGAAGCGCGGGTTTCGGTTGCGGTTTTCTACAACCCTCGAATCAGAGACAGCTTGTACGGACCACTCCCAGAGCTAATCTCGCAGGACACACCTGCAGTTTATCGCCACTTCACTTATGATGATTACATAACAAGGTTCTTCAACAAGGAGTTGGATGGCAAATCTTTGCCCAACCATTATAGAATTTGA

Protein sequence

MHTLCNSNPLSIFQPTFPSKNPNQTSKRKFHIVKMAADFDRATELKQFDQTKAGVKGLLDAGLKQIPRIFIHPPETLSDLKPTLKSPSSAAVVVPTIDLAGIHSPDLRPSIVDQIRNAASTFGFFQIINHGVPQKVLDEILDSVRAFHELPSEAKSPYYRRDVGTGVSYLSNIDLFNAKAASWRDTIQIGLGPVAAATENIPETCRAAVAEWAVETERLGYLLEEVLCEGLGLKSERLKEATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVEPVAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEARVSVAVFYNPRIRDSLYGPLPELISQDTPAVYRHFTYDDYITRFFNKELDGKSLPNHYRI
Homology
BLAST of Spg028742 vs. NCBI nr
Match: XP_038892530.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 3-like [Benincasa hispida])

HSP 1 Score: 731.5 bits (1887), Expect = 3.9e-207
Identity = 355/397 (89.42%), Postives = 374/397 (94.21%), Query Frame = 0

Query: 1   MHTLCNSNPLSIFQPTFPSKNPNQTSKRKFHIVKMAADFDRATELKQFDQTKAGVKGLLD 60
           M +LCNSN LS+ Q TFPSKNPN+T K KFHIVKMAADFDRATELKQFD+TKAGVKGLLD
Sbjct: 1   MLSLCNSNSLSLLQTTFPSKNPNRTPKNKFHIVKMAADFDRATELKQFDETKAGVKGLLD 60

Query: 61  AGLKQIPRIFIHPPETLSDLKPTLKSP--SSAAVVVPTIDLAGIHSPDLRPSIVDQIRNA 120
           AG+ QIP IFIHPPETLSDLKP+ KSP  SSAAVV+PTIDLAG+ SPDLRPSIVDQ+RNA
Sbjct: 61  AGINQIPHIFIHPPETLSDLKPSSKSPSSSSAAVVIPTIDLAGLDSPDLRPSIVDQVRNA 120

Query: 121 ASTFGFFQIINHGVPQKVLDEILDSVRAFHELPSEAKSPYYRRDVGTGVSYLSNIDLFNA 180
           ASTFGFFQIINHGVPQKVLD+ILDS+RAFHELPSEAKSPYYRRDVGTGVSYLSNIDLF A
Sbjct: 121 ASTFGFFQIINHGVPQKVLDDILDSIRAFHELPSEAKSPYYRRDVGTGVSYLSNIDLFKA 180

Query: 181 KAASWRDTIQIGLGPVAAATENIPETCRAAVAEWAVETERLGYLLEEVLCEGLGLKSERL 240
           KAASWRDT+Q+GLGPVAAA ENIPE CRAAV EWA ETE+LGYLLEEVLCEGLGL+ ERL
Sbjct: 181 KAASWRDTLQVGLGPVAAAAENIPEICRAAVGEWAKETEKLGYLLEEVLCEGLGLRPERL 240

Query: 241 KEATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVE 300
           KEATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVE
Sbjct: 241 KEATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVE 300

Query: 301 PVAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEARVSVAVFYNPRIRDSLYGPLPEL 360
           PVA ALVINIGDLLQIASN+VYKSVEHRVLANGKSEARVSVAVFYNPRIRD+LYGP PEL
Sbjct: 301 PVACALVINIGDLLQIASNNVYKSVEHRVLANGKSEARVSVAVFYNPRIRDNLYGPFPEL 360

Query: 361 ISQDTPAVYRHFTYDDYITRFFNKELDGKSLPNHYRI 396
           ISQDTPA Y+ FTY DYITRFFNKELDGKSLPNHY+I
Sbjct: 361 ISQDTPAAYQQFTYTDYITRFFNKELDGKSLPNHYKI 397

BLAST of Spg028742 vs. NCBI nr
Match: XP_022986647.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 3-like [Cucurbita maxima])

HSP 1 Score: 716.8 bits (1849), Expect = 9.9e-203
Identity = 348/397 (87.66%), Postives = 372/397 (93.70%), Query Frame = 0

Query: 1   MHTLCNSNPLSIFQPTFPSKNPNQTSKRKFHIVKMAADFDRATELKQFDQTKAGVKGLLD 60
           MH LCNS PLS+FQPTFP++N ++TS  K HIVKMA DFDRA ELKQFD+TKAGVKGLLD
Sbjct: 1   MHGLCNSKPLSLFQPTFPTRNHSRTSINKLHIVKMATDFDRAAELKQFDETKAGVKGLLD 60

Query: 61  AGLKQIPRIFIHPPETLSDLKPTLKSPSSAAVVVPTIDLAGIHSPDLRPSIVDQIRNAAS 120
           AGL QIPRIFIHPPETLSDLKPTLKS S  AVV+PTIDLAG+ SPDLRPSIVDQIRNA+S
Sbjct: 61  AGLNQIPRIFIHPPETLSDLKPTLKS-SGEAVVIPTIDLAGLDSPDLRPSIVDQIRNASS 120

Query: 121 TFGFFQIINHGVPQKVLDEILDSVRAFHELPSEAKSPYYRRDVGTGVSYLSNIDLFNAKA 180
           TFGFFQIINHGVPQKVLD+I+DS+RAFHELPSEAKSPYY+R+VGTGVSYLSNIDLF AKA
Sbjct: 121 TFGFFQIINHGVPQKVLDDIIDSIRAFHELPSEAKSPYYQRNVGTGVSYLSNIDLFKAKA 180

Query: 181 ASWRDTIQIGLGP--VAAATENIPETCRAAVAEWAVETERLGYLLEEVLCEGLGLKSERL 240
           ASWRDT+QIG+GP   AAA ENIPE CRAAVAEWA ETERLG++LEE+L EGLGLKSERL
Sbjct: 181 ASWRDTLQIGIGPAKAAAAAENIPEVCRAAVAEWASETERLGFVLEELLSEGLGLKSERL 240

Query: 241 KEATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVE 300
           KEATYGE RMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVE
Sbjct: 241 KEATYGERRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVE 300

Query: 301 PVAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEARVSVAVFYNPRIRDSLYGPLPEL 360
           PVAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEAR+S+AVFYNP IRD+LYGPLPEL
Sbjct: 301 PVAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEARISIAVFYNPEIRDNLYGPLPEL 360

Query: 361 ISQDTPAVYRHFTYDDYITRFFNKELDGKSLPNHYRI 396
           I+ +TPAVYR FTYDDYITRFFNKELDGKSLPNHYRI
Sbjct: 361 ITPETPAVYRQFTYDDYITRFFNKELDGKSLPNHYRI 396

BLAST of Spg028742 vs. NCBI nr
Match: XP_008464717.1 (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 [Cucumis melo])

HSP 1 Score: 709.5 bits (1830), Expect = 1.6e-200
Identity = 347/396 (87.63%), Postives = 366/396 (92.42%), Query Frame = 0

Query: 1   MHTLCNSNPLSIFQPTFPSKNPNQTSKRKFHIVKMAADFDRATELKQFDQTKAGVKGLLD 60
           M TLCNSNPLS+ Q TFPSKN   T   KF+ +KMAADFDRATELKQFDQTKAGVKGLLD
Sbjct: 1   MLTLCNSNPLSLLQTTFPSKNTITTPNNKFYTLKMAADFDRATELKQFDQTKAGVKGLLD 60

Query: 61  AGLKQIPRIFIHPPETLSDLKPTLKSPSSA-AVVVPTIDLAGIHSPDLRPSIVDQIRNAA 120
           AG+ QIPRIFIHPPETLSDLKPT KSPSS+  V+VPTIDLA +HSPD R SIVD IR+AA
Sbjct: 61  AGINQIPRIFIHPPETLSDLKPTSKSPSSSPPVLVPTIDLAALHSPDNRSSIVDHIRDAA 120

Query: 121 STFGFFQIINHGVPQKVLDEILDSVRAFHELPSEAKSPYYRRDVGTGVSYLSNIDLFNAK 180
           STFGFFQIINHGVPQKVLD+ILDS+RAFHELP+EAKS YYRRDV TGVSYLSNIDLF AK
Sbjct: 121 STFGFFQIINHGVPQKVLDDILDSIRAFHELPAEAKSSYYRRDVETGVSYLSNIDLFKAK 180

Query: 181 AASWRDTIQIGLGPVAAATENIPETCRAAVAEWAVETERLGYLLEEVLCEGLGLKSERLK 240
           AASWRDT+QIGLGP+AAA+ENIP  CR AVAEWA ETERLGYLLEE+LCEGLGL+SERLK
Sbjct: 181 AASWRDTLQIGLGPMAAASENIPAICRKAVAEWAKETERLGYLLEELLCEGLGLRSERLK 240

Query: 241 EATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVEP 300
           EATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVEP
Sbjct: 241 EATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVEP 300

Query: 301 VAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEARVSVAVFYNPRIRDSLYGPLPELI 360
           V GALVINIGDLLQIASNDVYKSVEHRVLANGK EARVSVAVFYNPRIRD+LYGP PELI
Sbjct: 301 VPGALVINIGDLLQIASNDVYKSVEHRVLANGKPEARVSVAVFYNPRIRDNLYGPFPELI 360

Query: 361 SQDTPAVYRHFTYDDYITRFFNKELDGKSLPNHYRI 396
           SQDTPAVY+ FTY DYITRFFNKELDGKSLPNHYR+
Sbjct: 361 SQDTPAVYQQFTYADYITRFFNKELDGKSLPNHYRV 396

BLAST of Spg028742 vs. NCBI nr
Match: XP_022943220.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 3-like [Cucurbita moschata])

HSP 1 Score: 709.1 bits (1829), Expect = 2.1e-200
Identity = 343/397 (86.40%), Postives = 370/397 (93.20%), Query Frame = 0

Query: 1   MHTLCNSNPLSIFQPTFPSKNPNQTSKRKFHIVKMAADFDRATELKQFDQTKAGVKGLLD 60
           MH LCNS PLS+FQPTFP++N  +TS  K HIVKMA DFDRA ELKQFD+TKAGVKGLLD
Sbjct: 1   MHGLCNSKPLSLFQPTFPTRNHIRTSINKLHIVKMATDFDRAAELKQFDETKAGVKGLLD 60

Query: 61  AGLKQIPRIFIHPPETLSDLKPTLKSPSSAAVVVPTIDLAGIHSPDLRPSIVDQIRNAAS 120
           AGL +IPRIFIHPPETLSDLKPT KS S  AVV+PTIDLAG++SPDLRPSIVDQIRNA+S
Sbjct: 61  AGLNKIPRIFIHPPETLSDLKPTPKS-SGEAVVIPTIDLAGLYSPDLRPSIVDQIRNASS 120

Query: 121 TFGFFQIINHGVPQKVLDEILDSVRAFHELPSEAKSPYYRRDVGTGVSYLSNIDLFNAKA 180
           TFGFFQIINHGVPQKVLD+++DS+RAFHELPSEAKSPYY+RDVGTGVSYLSNIDLF AKA
Sbjct: 121 TFGFFQIINHGVPQKVLDDVIDSIRAFHELPSEAKSPYYQRDVGTGVSYLSNIDLFKAKA 180

Query: 181 ASWRDTIQIGLGP--VAAATENIPETCRAAVAEWAVETERLGYLLEEVLCEGLGLKSERL 240
           ASWRDT+QIG+GP   AAA ENIPE CRAAVAEWA ETERLG++LEE+L EGLGLKSERL
Sbjct: 181 ASWRDTLQIGIGPAKAAAAAENIPEVCRAAVAEWASETERLGFVLEELLSEGLGLKSERL 240

Query: 241 KEATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVE 300
           KEATYGE RMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVE
Sbjct: 241 KEATYGERRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVE 300

Query: 301 PVAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEARVSVAVFYNPRIRDSLYGPLPEL 360
           PVAGALVINIGDLLQIASNDVYKSVEHRVLANGK+EAR+S+AVFYNP IRD+LYGP+PEL
Sbjct: 301 PVAGALVINIGDLLQIASNDVYKSVEHRVLANGKAEARISIAVFYNPEIRDNLYGPIPEL 360

Query: 361 ISQDTPAVYRHFTYDDYITRFFNKELDGKSLPNHYRI 396
           I+ +TPAVY  FTYDDYITRFFNKELDGKSLPNHYRI
Sbjct: 361 ITPETPAVYGQFTYDDYITRFFNKELDGKSLPNHYRI 396

BLAST of Spg028742 vs. NCBI nr
Match: XP_004142373.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 6 isoform X1 [Cucumis sativus] >KGN52352.1 hypothetical protein Csa_008161 [Cucumis sativus])

HSP 1 Score: 708.8 bits (1828), Expect = 2.7e-200
Identity = 348/396 (87.88%), Postives = 366/396 (92.42%), Query Frame = 0

Query: 1   MHTLCNSNPLSIFQPTFPSKNPNQTSKRKFHIVKMAADFDRATELKQFDQTKAGVKGLLD 60
           M +L NSNPLS+ Q  FPSKNPN T K KF+IVKMAADFDRATELKQFD+TKAGVKGLLD
Sbjct: 1   MLSLFNSNPLSLLQTKFPSKNPNPTPKHKFYIVKMAADFDRATELKQFDETKAGVKGLLD 60

Query: 61  AGLKQIPRIFIHPPETLSDLKPTLKSPSSAA-VVVPTIDLAGIHSPDLRPSIVDQIRNAA 120
           AG+ QIPRIFIHPPETLSDLKPT KSPSS+  VV+PTIDLAG+HSPD R SIV  IRNAA
Sbjct: 61  AGINQIPRIFIHPPETLSDLKPTSKSPSSSTPVVIPTIDLAGLHSPDNRSSIVGHIRNAA 120

Query: 121 STFGFFQIINHGVPQKVLDEILDSVRAFHELPSEAKSPYYRRDVGTGVSYLSNIDLFNAK 180
           STFGFFQIINHGVPQKVLD+ILDS+RAFHELP+EAKS YYRRDV TGVSYLSNIDLF AK
Sbjct: 121 STFGFFQIINHGVPQKVLDDILDSIRAFHELPAEAKSSYYRRDVETGVSYLSNIDLFKAK 180

Query: 181 AASWRDTIQIGLGPVAAATENIPETCRAAVAEWAVETERLGYLLEEVLCEGLGLKSERLK 240
           AASWRDT+QIGLGPVAAA ENIP  CR A+AEWA ETERLGYLLEEVLCEGLGL+SERLK
Sbjct: 181 AASWRDTLQIGLGPVAAAPENIPAICREAMAEWAKETERLGYLLEEVLCEGLGLRSERLK 240

Query: 241 EATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVEP 300
           EATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRH GGWVDV+P
Sbjct: 241 EATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHDGGWVDVKP 300

Query: 301 VAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEARVSVAVFYNPRIRDSLYGPLPELI 360
           VAGALVINIGDLLQI SNDVYKSVEHRVLANGK+EARVSVAVFYNPRIRD+LYGP PELI
Sbjct: 301 VAGALVINIGDLLQIVSNDVYKSVEHRVLANGKAEARVSVAVFYNPRIRDNLYGPFPELI 360

Query: 361 SQDTPAVYRHFTYDDYITRFFNKELDGKSLPNHYRI 396
           SQ TPAVY+ FTY DYITRFFNKELDGKSLPNHYRI
Sbjct: 361 SQVTPAVYQQFTYADYITRFFNKELDGKSLPNHYRI 396

BLAST of Spg028742 vs. ExPASy Swiss-Prot
Match: Q8H1S4 (1-aminocyclopropane-1-carboxylate oxidase homolog 3 OS=Arabidopsis thaliana OX=3702 GN=At1g06650 PE=2 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.8e-91
Identity = 172/368 (46.74%), Postives = 235/368 (63.86%), Query Frame = 0

Query: 32  IVKMAADFDRATELKQFDQTKAGVKGLLDAGLKQIPRIFIHPPETLSDLKPTLKSPSSAA 91
           ++K+   FDRA+ELK FD+TK GVKGL+D+G+ Q+PRIF HP   LS  KP L S     
Sbjct: 3   MMKIDPLFDRASELKAFDETKTGVKGLVDSGVSQVPRIFHHPTVKLSTPKP-LPSDLLHL 62

Query: 92  VVVPTIDLAGIHSPDL--RPSIVDQIRNAASTFGFFQIINHGVPQKVLDEILDSVRAFHE 151
             +PTIDL G    D   R + +++I+ AA+ +GFFQ+INHGV  ++L+++   VR FHE
Sbjct: 63  KTIPTIDLGGRDFQDAIKRNNAIEEIKEAAAKWGFFQVINHGVSLELLEKMKKGVRDFHE 122

Query: 152 LPSEAKSPYYRRDVGTGVSYLSNIDLFNAKAASWRDTIQIGLGPVAAATENIPETCRAAV 211
              E +  +Y RD      YLSN DLF++ AA+WRDT    + P     +++PE CR  +
Sbjct: 123 QSQEVRKEFYSRDFSRRFLYLSNFDLFSSPAANWRDTFSCTMAPDTPKPQDLPEICRDIM 182

Query: 212 AEWAVETERLGYLLEEVLCEGLGLKSERLKEATYGEGRMMVGHYYPYCPQPDLTVGIKSH 271
            E++ +   LG  L E+L E LGL+   L +    +G +M+ HYYP CP+PDLT+G   H
Sbjct: 183 MEYSKQVMNLGKFLFELLSEALGLEPNHLNDMDCSKGLLMLSHYYPPCPEPDLTLGTSQH 242

Query: 272 TDPGVLTVLLQDHIGGLQIRHGGGWVDVEPVAGALVINIGDLLQIASNDVYKSVEHRVLA 331
           +D   LTVLL D I GLQ+R  G W DV  V+GAL+INIGDLLQ+ +ND + S+EHRVLA
Sbjct: 243 SDNSFLTVLLPDQIEGLQVRREGHWFDVPHVSGALIINIGDLLQLITNDKFISLEHRVLA 302

Query: 332 NGKSEARVSVAVFYNPRIRDS--LYGPLPELISQDTPAVYRHFTYDDYITRFFNKELDGK 391
           N  + ARVSVA F+   +R +  +YGP+ EL+S++ P  YR  T  DY T F  K LDG 
Sbjct: 303 NRATRARVSVACFFTTGVRPNPRMYGPIRELVSEENPPKYRETTIKDYATYFNAKGLDGT 362

Query: 392 SLPNHYRI 396
           S   H++I
Sbjct: 363 SALLHFKI 369

BLAST of Spg028742 vs. ExPASy Swiss-Prot
Match: Q84MB3 (1-aminocyclopropane-1-carboxylate oxidase homolog 1 OS=Arabidopsis thaliana OX=3702 GN=At1g06620 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 6.3e-91
Identity = 172/359 (47.91%), Postives = 236/359 (65.74%), Query Frame = 0

Query: 37  ADFDRATELKQFDQTKAGVKGLLDAGLKQIPRIFIHPPETLSDLKPTLKSPSSAAVVVPT 96
           A  DR+T LK FD+TK GVKGL+DAG+ +IP IF  PP TL+  KP    PSS+   +PT
Sbjct: 9   AALDRSTLLKAFDETKTGVKGLIDAGITEIPSIFRAPPATLTSPKP----PSSSDFSIPT 68

Query: 97  IDLAGIHSPDL-RPSIVDQIRNAASTFGFFQIINHGVPQKVLDEILDSVRAFHELPSEAK 156
           IDL G  +  + R S+V++I +AA  +GFFQ+INHG+P  VL++++D +R FHE  +E K
Sbjct: 69  IDLKGGGTDSITRRSLVEKIGDAAEKWGFFQVINHGIPMDVLEKMIDGIREFHEQDTEVK 128

Query: 157 SPYYRRDVGTGVSYLSNIDLFNAKAASWRDTIQIGLGPVAAATENIPETCRAAVAEWAVE 216
             +Y RD  + + Y SN DLF++ AA+WRDT+     P     E++P TC   + E++ E
Sbjct: 129 KGFYSRDPASKMVYSSNFDLFSSPAANWRDTLGCYTAPDPPRPEDLPATCGEMMIEYSKE 188

Query: 217 TERLGYLLEEVLCEGLGLKSERLKEATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVL 276
             +LG LL E+L E LGL +  LK+       +++GHYYP CPQPDLT+G+  H+D   L
Sbjct: 189 VMKLGKLLFELLSEALGLNTNHLKDMDCTNSLLLLGHYYPPCPQPDLTLGLTKHSDNSFL 248

Query: 277 TVLLQDHIGGLQIRHGGGWVDVEPVAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEA 336
           T+LLQDHIGGLQ+ H   WVDV PV GALV+N+GDLLQ+ +ND + SVEHRVLAN  +  
Sbjct: 249 TILLQDHIGGLQVLHDQYWVDVPPVPGALVVNVGDLLQLITNDKFISVEHRVLAN-VAGP 308

Query: 337 RVSVAVFY------NPRIRDSLYGPLPELISQDTPAVYRHFTYDDYITRFFNKELDGKS 389
           R+SVA F+      NPR+    YGP+ E++S++ P  YR  T  +Y   + +K  DG S
Sbjct: 309 RISVACFFSSYLMANPRV----YGPIKEILSEENPPNYRDTTITEYAKFYRSKGFDGTS 358

BLAST of Spg028742 vs. ExPASy Swiss-Prot
Match: P93824 (1-aminocyclopropane-1-carboxylate oxidase homolog 6 OS=Arabidopsis thaliana OX=3702 GN=At1g04350 PE=2 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.4e-90
Identity = 172/361 (47.65%), Postives = 233/361 (64.54%), Query Frame = 0

Query: 38  DFDRATELKQFDQTKAGVKGLLDAGLKQIPRIFIHPPETLSDLKPTLKSPSSAAVVVPTI 97
           +FD  +E K FD+TK GVKGL+DA + +IPRIF  P  +LSD KP +   S+    +P I
Sbjct: 5   EFDSYSERKAFDETKTGVKGLIDAHITEIPRIFCLPQGSLSDKKPFV---STTDFAIPII 64

Query: 98  DLAGIHSPDLRPSIVDQIRNAASTFGFFQIINHGVPQKVLDEILDSVRAFHELPSEAKSP 157
           D  G+H    R  IV +I++AAS +GFFQ+INHGVP  VL EI D VR FHE   E K  
Sbjct: 65  DFEGLHVS--REDIVGKIKDAASNWGFFQVINHGVPLNVLQEIQDGVRRFHEEAPEVKKT 124

Query: 158 YYRRDVGTGVSYLSNIDLFNAKA-ASWRDTIQIGLGPVAAATENIPETCRAAVAEWAVET 217
           Y+ RD      Y SN DL+++ +  +WRD+    + P     E++P  CR A+ E++   
Sbjct: 125 YFTRDATKRFVYNSNFDLYSSSSCVNWRDSFACYMAPDPPNPEDLPVACRVAMFEYSKHM 184

Query: 218 ERLGYLLEEVLCEGLGLKSERLKEATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLT 277
            RLG LL E+L E LGL+S++LK     +G +++ HYYP CPQPDLT+G  +H+D   LT
Sbjct: 185 MRLGDLLFELLSEALGLRSDKLKSMDCMKGLLLLCHYYPPCPQPDLTIGTNNHSDNSFLT 244

Query: 278 VLLQDHIGGLQIRHGGGWVDVEPVAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEAR 337
           +LLQD IGGLQI H   WVDV P+ GALVIN+GD LQ+ +ND   SVEHRVLAN  +  R
Sbjct: 245 ILLQDQIGGLQIFHQDCWVDVSPIPGALVINMGDFLQLITNDKVISVEHRVLANRAATPR 304

Query: 338 VSVAVFYNPRIR--DSLYGPLPELISQDTPAVYRHFTYDDYITRFFNKELDGKSLPNHYR 396
           +SVA F++  +R   ++YGP+ EL+S++ P+ YR     +Y   +F K LDG S  +HY+
Sbjct: 305 ISVASFFSTSMRPNSTVYGPIKELLSEENPSKYRVIDLKEYTEGYFKKGLDGTSYLSHYK 360

BLAST of Spg028742 vs. ExPASy Swiss-Prot
Match: Q9LTH8 (1-aminocyclopropane-1-carboxylate oxidase homolog 11 OS=Arabidopsis thaliana OX=3702 GN=At5g59530 PE=2 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 3.8e-88
Identity = 176/370 (47.57%), Postives = 237/370 (64.05%), Query Frame = 0

Query: 32  IVKMAADFDRATELKQFDQTKAGVKGLLDAGLKQIPRIFIHPPETLSDLKPTLKSPSSAA 91
           + K + +FDR  E K FD TK GVKGL+DA + +IPRIF  P +TL D     K  S + 
Sbjct: 1   MAKNSVEFDRYIERKAFDNTKEGVKGLIDAKITEIPRIFHVPQDTLPD-----KKRSVSD 60

Query: 92  VVVPTIDLAGIH-SPDLRPSIVDQIRNAASTFGFFQIINHGVPQKVLDEILDSVRAFHEL 151
           + +PTID A ++     R +IV++++ A   +GFFQ+INHGVP  VL+EI D VR FHE 
Sbjct: 61  LEIPTIDFASVNVDTPSREAIVEKVKYAVENWGFFQVINHGVPLNVLEEIKDGVRRFHEE 120

Query: 152 PS-EAKSPYYRRD-VGTGVSYLSNIDLFNAK-AASWRDTIQIGLGPVAAATENIPETCRA 211
              E K  YY  D      +Y SN DL+++  + +WRD+I   + P     E +PETCR 
Sbjct: 121 EDPEVKKSYYSLDFTKNKFAYSSNFDLYSSSPSLTWRDSISCYMAPDPPTPEELPETCRD 180

Query: 212 AVAEWAVETERLGYLLEEVLCEGLGLKSERLKEATYGEGRMMVGHYYPYCPQPDLTVGIK 271
           A+ E++     LG LL E+L E LGLKSE LK     +  +M+ HYYP CPQPDLT+GI 
Sbjct: 181 AMIEYSKHVLSLGDLLFELLSEALGLKSEILKSMDCLKSLLMICHYYPPCPQPDLTLGIS 240

Query: 272 SHTDPGVLTVLLQDHIGGLQIRHGGGWVDVEPVAGALVINIGDLLQIASNDVYKSVEHRV 331
            H+D   LTVLLQD+IGGLQI H   WVDV P+ GALV+N+GD LQ+ +ND + SVEHRV
Sbjct: 241 KHSDNSFLTVLLQDNIGGLQILHQDSWVDVSPLPGALVVNVGDFLQLITNDKFISVEHRV 300

Query: 332 LANGKSEARVSVAVFYNPRIRD--SLYGPLPELISQDTPAVYRHFTYDDYITRFFNKELD 391
           LAN +   R+SVA F++  IR+  ++YGP+ EL+S++ P  YR  T  +Y   +F K LD
Sbjct: 301 LANTRG-PRISVASFFSSSIRENSTVYGPMKELVSEENPPKYRDTTLREYSEGYFKKGLD 360

Query: 392 GKSLPNHYRI 396
           G S  +++RI
Sbjct: 361 GTSHLSNFRI 364

BLAST of Spg028742 vs. ExPASy Swiss-Prot
Match: Q9LTH7 (1-aminocyclopropane-1-carboxylate oxidase homolog 12 OS=Arabidopsis thaliana OX=3702 GN=At5g59540 PE=2 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 3.2e-87
Identity = 170/369 (46.07%), Postives = 236/369 (63.96%), Query Frame = 0

Query: 32  IVKMAADFDRATELKQFDQTKAGVKGLLDAGLKQIPRIFIHPPETLSDLKPTLKSPSSAA 91
           + K + +FD   E K FD+TK GVKGL+DA + ++PRIF H  + L++ KP   S S + 
Sbjct: 2   MTKNSIEFDPYIERKAFDETKQGVKGLVDAKITEVPRIFHHRQDILTNKKP---SASVSD 61

Query: 92  VVVPTIDLAGIHSPDL-RPSIVDQIRNAASTFGFFQIINHGVPQKVLDEILDSVRAFHEL 151
           + +P ID A +H+    R +IV++++ A   +GFFQ+INH +P  VL+EI D VR FHE 
Sbjct: 62  LEIPIIDFASVHADTASREAIVEKVKYAVENWGFFQVINHSIPLNVLEEIKDGVRRFHEE 121

Query: 152 PSEAKSPYYRRDVGT-GVSYLSNIDLFNAK-AASWRDTIQIGLGPVAAATENIPETCRAA 211
             E K  ++ RD G     Y SN DL+++  + +WRD+    + P   A E IPETCR A
Sbjct: 122 DPEVKKSFFSRDAGNKKFVYNSNFDLYSSSPSVNWRDSFSCYIAPDPPAPEEIPETCRDA 181

Query: 212 VAEWAVETERLGYLLEEVLCEGLGLKSERLKEATYGEGRMMVGHYYPYCPQPDLTVGIKS 271
           + E++      G LL E+L E LGLKS+ L+     +  +M+ HYYP CPQPDLT+GI  
Sbjct: 182 MFEYSKHVLSFGGLLFELLSEALGLKSQTLESMDCVKTLLMICHYYPPCPQPDLTLGITK 241

Query: 272 HTDPGVLTVLLQDHIGGLQIRHGGGWVDVEPVAGALVINIGDLLQIASNDVYKSVEHRVL 331
           H+D   LT+LLQD+IGGLQI H   WVDV P+ GALV+NIGD LQ+ +ND + SVEHRVL
Sbjct: 242 HSDNSFLTLLLQDNIGGLQILHQDSWVDVSPIHGALVVNIGDFLQLITNDKFVSVEHRVL 301

Query: 332 ANGKSEARVSVAVFYNPRIRDS--LYGPLPELISQDTPAVYRHFTYDDYITRFFNKELDG 391
           AN +   R+SVA F++  +R +  +YGP+ EL+S++ P  YR  T  +Y   FF K LDG
Sbjct: 302 AN-RQGPRISVASFFSSSMRPNSRVYGPMKELVSEENPPKYRDITIKEYSKIFFEKGLDG 361

Query: 392 KSLPNHYRI 396
            S  ++ RI
Sbjct: 362 TSHLSNIRI 366

BLAST of Spg028742 vs. ExPASy TrEMBL
Match: A0A6J1J854 (1-aminocyclopropane-1-carboxylate oxidase homolog 3-like OS=Cucurbita maxima OX=3661 GN=LOC111484321 PE=3 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 4.8e-203
Identity = 348/397 (87.66%), Postives = 372/397 (93.70%), Query Frame = 0

Query: 1   MHTLCNSNPLSIFQPTFPSKNPNQTSKRKFHIVKMAADFDRATELKQFDQTKAGVKGLLD 60
           MH LCNS PLS+FQPTFP++N ++TS  K HIVKMA DFDRA ELKQFD+TKAGVKGLLD
Sbjct: 1   MHGLCNSKPLSLFQPTFPTRNHSRTSINKLHIVKMATDFDRAAELKQFDETKAGVKGLLD 60

Query: 61  AGLKQIPRIFIHPPETLSDLKPTLKSPSSAAVVVPTIDLAGIHSPDLRPSIVDQIRNAAS 120
           AGL QIPRIFIHPPETLSDLKPTLKS S  AVV+PTIDLAG+ SPDLRPSIVDQIRNA+S
Sbjct: 61  AGLNQIPRIFIHPPETLSDLKPTLKS-SGEAVVIPTIDLAGLDSPDLRPSIVDQIRNASS 120

Query: 121 TFGFFQIINHGVPQKVLDEILDSVRAFHELPSEAKSPYYRRDVGTGVSYLSNIDLFNAKA 180
           TFGFFQIINHGVPQKVLD+I+DS+RAFHELPSEAKSPYY+R+VGTGVSYLSNIDLF AKA
Sbjct: 121 TFGFFQIINHGVPQKVLDDIIDSIRAFHELPSEAKSPYYQRNVGTGVSYLSNIDLFKAKA 180

Query: 181 ASWRDTIQIGLGP--VAAATENIPETCRAAVAEWAVETERLGYLLEEVLCEGLGLKSERL 240
           ASWRDT+QIG+GP   AAA ENIPE CRAAVAEWA ETERLG++LEE+L EGLGLKSERL
Sbjct: 181 ASWRDTLQIGIGPAKAAAAAENIPEVCRAAVAEWASETERLGFVLEELLSEGLGLKSERL 240

Query: 241 KEATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVE 300
           KEATYGE RMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVE
Sbjct: 241 KEATYGERRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVE 300

Query: 301 PVAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEARVSVAVFYNPRIRDSLYGPLPEL 360
           PVAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEAR+S+AVFYNP IRD+LYGPLPEL
Sbjct: 301 PVAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEARISIAVFYNPEIRDNLYGPLPEL 360

Query: 361 ISQDTPAVYRHFTYDDYITRFFNKELDGKSLPNHYRI 396
           I+ +TPAVYR FTYDDYITRFFNKELDGKSLPNHYRI
Sbjct: 361 ITPETPAVYRQFTYDDYITRFFNKELDGKSLPNHYRI 396

BLAST of Spg028742 vs. ExPASy TrEMBL
Match: A0A1S3CML8 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103502535 PE=3 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 7.7e-201
Identity = 347/396 (87.63%), Postives = 366/396 (92.42%), Query Frame = 0

Query: 1   MHTLCNSNPLSIFQPTFPSKNPNQTSKRKFHIVKMAADFDRATELKQFDQTKAGVKGLLD 60
           M TLCNSNPLS+ Q TFPSKN   T   KF+ +KMAADFDRATELKQFDQTKAGVKGLLD
Sbjct: 1   MLTLCNSNPLSLLQTTFPSKNTITTPNNKFYTLKMAADFDRATELKQFDQTKAGVKGLLD 60

Query: 61  AGLKQIPRIFIHPPETLSDLKPTLKSPSSA-AVVVPTIDLAGIHSPDLRPSIVDQIRNAA 120
           AG+ QIPRIFIHPPETLSDLKPT KSPSS+  V+VPTIDLA +HSPD R SIVD IR+AA
Sbjct: 61  AGINQIPRIFIHPPETLSDLKPTSKSPSSSPPVLVPTIDLAALHSPDNRSSIVDHIRDAA 120

Query: 121 STFGFFQIINHGVPQKVLDEILDSVRAFHELPSEAKSPYYRRDVGTGVSYLSNIDLFNAK 180
           STFGFFQIINHGVPQKVLD+ILDS+RAFHELP+EAKS YYRRDV TGVSYLSNIDLF AK
Sbjct: 121 STFGFFQIINHGVPQKVLDDILDSIRAFHELPAEAKSSYYRRDVETGVSYLSNIDLFKAK 180

Query: 181 AASWRDTIQIGLGPVAAATENIPETCRAAVAEWAVETERLGYLLEEVLCEGLGLKSERLK 240
           AASWRDT+QIGLGP+AAA+ENIP  CR AVAEWA ETERLGYLLEE+LCEGLGL+SERLK
Sbjct: 181 AASWRDTLQIGLGPMAAASENIPAICRKAVAEWAKETERLGYLLEELLCEGLGLRSERLK 240

Query: 241 EATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVEP 300
           EATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVEP
Sbjct: 241 EATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVEP 300

Query: 301 VAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEARVSVAVFYNPRIRDSLYGPLPELI 360
           V GALVINIGDLLQIASNDVYKSVEHRVLANGK EARVSVAVFYNPRIRD+LYGP PELI
Sbjct: 301 VPGALVINIGDLLQIASNDVYKSVEHRVLANGKPEARVSVAVFYNPRIRDNLYGPFPELI 360

Query: 361 SQDTPAVYRHFTYDDYITRFFNKELDGKSLPNHYRI 396
           SQDTPAVY+ FTY DYITRFFNKELDGKSLPNHYR+
Sbjct: 361 SQDTPAVYQQFTYADYITRFFNKELDGKSLPNHYRV 396

BLAST of Spg028742 vs. ExPASy TrEMBL
Match: A0A6J1FXJ9 (1-aminocyclopropane-1-carboxylate oxidase homolog 3-like OS=Cucurbita moschata OX=3662 GN=LOC111448015 PE=3 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 1.0e-200
Identity = 343/397 (86.40%), Postives = 370/397 (93.20%), Query Frame = 0

Query: 1   MHTLCNSNPLSIFQPTFPSKNPNQTSKRKFHIVKMAADFDRATELKQFDQTKAGVKGLLD 60
           MH LCNS PLS+FQPTFP++N  +TS  K HIVKMA DFDRA ELKQFD+TKAGVKGLLD
Sbjct: 1   MHGLCNSKPLSLFQPTFPTRNHIRTSINKLHIVKMATDFDRAAELKQFDETKAGVKGLLD 60

Query: 61  AGLKQIPRIFIHPPETLSDLKPTLKSPSSAAVVVPTIDLAGIHSPDLRPSIVDQIRNAAS 120
           AGL +IPRIFIHPPETLSDLKPT KS S  AVV+PTIDLAG++SPDLRPSIVDQIRNA+S
Sbjct: 61  AGLNKIPRIFIHPPETLSDLKPTPKS-SGEAVVIPTIDLAGLYSPDLRPSIVDQIRNASS 120

Query: 121 TFGFFQIINHGVPQKVLDEILDSVRAFHELPSEAKSPYYRRDVGTGVSYLSNIDLFNAKA 180
           TFGFFQIINHGVPQKVLD+++DS+RAFHELPSEAKSPYY+RDVGTGVSYLSNIDLF AKA
Sbjct: 121 TFGFFQIINHGVPQKVLDDVIDSIRAFHELPSEAKSPYYQRDVGTGVSYLSNIDLFKAKA 180

Query: 181 ASWRDTIQIGLGP--VAAATENIPETCRAAVAEWAVETERLGYLLEEVLCEGLGLKSERL 240
           ASWRDT+QIG+GP   AAA ENIPE CRAAVAEWA ETERLG++LEE+L EGLGLKSERL
Sbjct: 181 ASWRDTLQIGIGPAKAAAAAENIPEVCRAAVAEWASETERLGFVLEELLSEGLGLKSERL 240

Query: 241 KEATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVE 300
           KEATYGE RMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVE
Sbjct: 241 KEATYGERRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVE 300

Query: 301 PVAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEARVSVAVFYNPRIRDSLYGPLPEL 360
           PVAGALVINIGDLLQIASNDVYKSVEHRVLANGK+EAR+S+AVFYNP IRD+LYGP+PEL
Sbjct: 301 PVAGALVINIGDLLQIASNDVYKSVEHRVLANGKAEARISIAVFYNPEIRDNLYGPIPEL 360

Query: 361 ISQDTPAVYRHFTYDDYITRFFNKELDGKSLPNHYRI 396
           I+ +TPAVY  FTYDDYITRFFNKELDGKSLPNHYRI
Sbjct: 361 ITPETPAVYGQFTYDDYITRFFNKELDGKSLPNHYRI 396

BLAST of Spg028742 vs. ExPASy TrEMBL
Match: A0A0A0KV77 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G627090 PE=3 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 1.3e-200
Identity = 348/396 (87.88%), Postives = 366/396 (92.42%), Query Frame = 0

Query: 1   MHTLCNSNPLSIFQPTFPSKNPNQTSKRKFHIVKMAADFDRATELKQFDQTKAGVKGLLD 60
           M +L NSNPLS+ Q  FPSKNPN T K KF+IVKMAADFDRATELKQFD+TKAGVKGLLD
Sbjct: 1   MLSLFNSNPLSLLQTKFPSKNPNPTPKHKFYIVKMAADFDRATELKQFDETKAGVKGLLD 60

Query: 61  AGLKQIPRIFIHPPETLSDLKPTLKSPSSAA-VVVPTIDLAGIHSPDLRPSIVDQIRNAA 120
           AG+ QIPRIFIHPPETLSDLKPT KSPSS+  VV+PTIDLAG+HSPD R SIV  IRNAA
Sbjct: 61  AGINQIPRIFIHPPETLSDLKPTSKSPSSSTPVVIPTIDLAGLHSPDNRSSIVGHIRNAA 120

Query: 121 STFGFFQIINHGVPQKVLDEILDSVRAFHELPSEAKSPYYRRDVGTGVSYLSNIDLFNAK 180
           STFGFFQIINHGVPQKVLD+ILDS+RAFHELP+EAKS YYRRDV TGVSYLSNIDLF AK
Sbjct: 121 STFGFFQIINHGVPQKVLDDILDSIRAFHELPAEAKSSYYRRDVETGVSYLSNIDLFKAK 180

Query: 181 AASWRDTIQIGLGPVAAATENIPETCRAAVAEWAVETERLGYLLEEVLCEGLGLKSERLK 240
           AASWRDT+QIGLGPVAAA ENIP  CR A+AEWA ETERLGYLLEEVLCEGLGL+SERLK
Sbjct: 181 AASWRDTLQIGLGPVAAAPENIPAICREAMAEWAKETERLGYLLEEVLCEGLGLRSERLK 240

Query: 241 EATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVEP 300
           EATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRH GGWVDV+P
Sbjct: 241 EATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHDGGWVDVKP 300

Query: 301 VAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEARVSVAVFYNPRIRDSLYGPLPELI 360
           VAGALVINIGDLLQI SNDVYKSVEHRVLANGK+EARVSVAVFYNPRIRD+LYGP PELI
Sbjct: 301 VAGALVINIGDLLQIVSNDVYKSVEHRVLANGKAEARVSVAVFYNPRIRDNLYGPFPELI 360

Query: 361 SQDTPAVYRHFTYDDYITRFFNKELDGKSLPNHYRI 396
           SQ TPAVY+ FTY DYITRFFNKELDGKSLPNHYRI
Sbjct: 361 SQVTPAVYQQFTYADYITRFFNKELDGKSLPNHYRI 396

BLAST of Spg028742 vs. ExPASy TrEMBL
Match: A0A1S4E5J8 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502535 PE=3 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 4.2e-199
Identity = 347/400 (86.75%), Postives = 366/400 (91.50%), Query Frame = 0

Query: 1   MHTLCNSNPLSIFQPTFPSKNPNQTSKRKFHIVKMAADFDRATELKQFDQTKAGVKGLLD 60
           M TLCNSNPLS+ Q TFPSKN   T   KF+ +KMAADFDRATELKQFDQTKAGVKGLLD
Sbjct: 1   MLTLCNSNPLSLLQTTFPSKNTITTPNNKFYTLKMAADFDRATELKQFDQTKAGVKGLLD 60

Query: 61  AGLKQIPRIFIHPPETLSDLKPTLKSPSSA-AVVVPTIDLAGIHSPDLRPSIVDQIRNAA 120
           AG+ QIPRIFIHPPETLSDLKPT KSPSS+  V+VPTIDLA +HSPD R SIVD IR+AA
Sbjct: 61  AGINQIPRIFIHPPETLSDLKPTSKSPSSSPPVLVPTIDLAALHSPDNRSSIVDHIRDAA 120

Query: 121 STFGFFQIINHGVPQKVLDEILDSVRAFHELPSEAKSPYYRRDVGTGVSYLSNIDLFNAK 180
           STFGFFQIINHGVPQKVLD+ILDS+RAFHELP+EAKS YYRRDV TGVSYLSNIDLF AK
Sbjct: 121 STFGFFQIINHGVPQKVLDDILDSIRAFHELPAEAKSSYYRRDVETGVSYLSNIDLFKAK 180

Query: 181 AASWRDTIQIGLGPVAAATENIPETCRAAVAEWAVETERLGYLLEEVLCEGLGLKSERLK 240
           AASWRDT+QIGLGP+AAA+ENIP  CR AVAEWA ETERLGYLLEE+LCEGLGL+SERLK
Sbjct: 181 AASWRDTLQIGLGPMAAASENIPAICRKAVAEWAKETERLGYLLEELLCEGLGLRSERLK 240

Query: 241 EATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVEP 300
           EATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVEP
Sbjct: 241 EATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLTVLLQDHIGGLQIRHGGGWVDVEP 300

Query: 301 VAGALVINIGDL----LQIASNDVYKSVEHRVLANGKSEARVSVAVFYNPRIRDSLYGPL 360
           V GALVINIGDL    LQIASNDVYKSVEHRVLANGK EARVSVAVFYNPRIRD+LYGP 
Sbjct: 301 VPGALVINIGDLLQFWLQIASNDVYKSVEHRVLANGKPEARVSVAVFYNPRIRDNLYGPF 360

Query: 361 PELISQDTPAVYRHFTYDDYITRFFNKELDGKSLPNHYRI 396
           PELISQDTPAVY+ FTY DYITRFFNKELDGKSLPNHYR+
Sbjct: 361 PELISQDTPAVYQQFTYADYITRFFNKELDGKSLPNHYRV 400

BLAST of Spg028742 vs. TAIR 10
Match: AT1G06650.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 337.0 bits (863), Expect = 2.0e-92
Identity = 172/368 (46.74%), Postives = 235/368 (63.86%), Query Frame = 0

Query: 32  IVKMAADFDRATELKQFDQTKAGVKGLLDAGLKQIPRIFIHPPETLSDLKPTLKSPSSAA 91
           ++K+   FDRA+ELK FD+TK GVKGL+D+G+ Q+PRIF HP   LS  KP L S     
Sbjct: 3   MMKIDPLFDRASELKAFDETKTGVKGLVDSGVSQVPRIFHHPTVKLSTPKP-LPSDLLHL 62

Query: 92  VVVPTIDLAGIHSPDL--RPSIVDQIRNAASTFGFFQIINHGVPQKVLDEILDSVRAFHE 151
             +PTIDL G    D   R + +++I+ AA+ +GFFQ+INHGV  ++L+++   VR FHE
Sbjct: 63  KTIPTIDLGGRDFQDAIKRNNAIEEIKEAAAKWGFFQVINHGVSLELLEKMKKGVRDFHE 122

Query: 152 LPSEAKSPYYRRDVGTGVSYLSNIDLFNAKAASWRDTIQIGLGPVAAATENIPETCRAAV 211
              E +  +Y RD      YLSN DLF++ AA+WRDT    + P     +++PE CR  +
Sbjct: 123 QSQEVRKEFYSRDFSRRFLYLSNFDLFSSPAANWRDTFSCTMAPDTPKPQDLPEICRDIM 182

Query: 212 AEWAVETERLGYLLEEVLCEGLGLKSERLKEATYGEGRMMVGHYYPYCPQPDLTVGIKSH 271
            E++ +   LG  L E+L E LGL+   L +    +G +M+ HYYP CP+PDLT+G   H
Sbjct: 183 MEYSKQVMNLGKFLFELLSEALGLEPNHLNDMDCSKGLLMLSHYYPPCPEPDLTLGTSQH 242

Query: 272 TDPGVLTVLLQDHIGGLQIRHGGGWVDVEPVAGALVINIGDLLQIASNDVYKSVEHRVLA 331
           +D   LTVLL D I GLQ+R  G W DV  V+GAL+INIGDLLQ+ +ND + S+EHRVLA
Sbjct: 243 SDNSFLTVLLPDQIEGLQVRREGHWFDVPHVSGALIINIGDLLQLITNDKFISLEHRVLA 302

Query: 332 NGKSEARVSVAVFYNPRIRDS--LYGPLPELISQDTPAVYRHFTYDDYITRFFNKELDGK 391
           N  + ARVSVA F+   +R +  +YGP+ EL+S++ P  YR  T  DY T F  K LDG 
Sbjct: 303 NRATRARVSVACFFTTGVRPNPRMYGPIRELVSEENPPKYRETTIKDYATYFNAKGLDGT 362

Query: 392 SLPNHYRI 396
           S   H++I
Sbjct: 363 SALLHFKI 369

BLAST of Spg028742 vs. TAIR 10
Match: AT1G06620.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 335.9 bits (860), Expect = 4.4e-92
Identity = 172/359 (47.91%), Postives = 236/359 (65.74%), Query Frame = 0

Query: 37  ADFDRATELKQFDQTKAGVKGLLDAGLKQIPRIFIHPPETLSDLKPTLKSPSSAAVVVPT 96
           A  DR+T LK FD+TK GVKGL+DAG+ +IP IF  PP TL+  KP    PSS+   +PT
Sbjct: 9   AALDRSTLLKAFDETKTGVKGLIDAGITEIPSIFRAPPATLTSPKP----PSSSDFSIPT 68

Query: 97  IDLAGIHSPDL-RPSIVDQIRNAASTFGFFQIINHGVPQKVLDEILDSVRAFHELPSEAK 156
           IDL G  +  + R S+V++I +AA  +GFFQ+INHG+P  VL++++D +R FHE  +E K
Sbjct: 69  IDLKGGGTDSITRRSLVEKIGDAAEKWGFFQVINHGIPMDVLEKMIDGIREFHEQDTEVK 128

Query: 157 SPYYRRDVGTGVSYLSNIDLFNAKAASWRDTIQIGLGPVAAATENIPETCRAAVAEWAVE 216
             +Y RD  + + Y SN DLF++ AA+WRDT+     P     E++P TC   + E++ E
Sbjct: 129 KGFYSRDPASKMVYSSNFDLFSSPAANWRDTLGCYTAPDPPRPEDLPATCGEMMIEYSKE 188

Query: 217 TERLGYLLEEVLCEGLGLKSERLKEATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVL 276
             +LG LL E+L E LGL +  LK+       +++GHYYP CPQPDLT+G+  H+D   L
Sbjct: 189 VMKLGKLLFELLSEALGLNTNHLKDMDCTNSLLLLGHYYPPCPQPDLTLGLTKHSDNSFL 248

Query: 277 TVLLQDHIGGLQIRHGGGWVDVEPVAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEA 336
           T+LLQDHIGGLQ+ H   WVDV PV GALV+N+GDLLQ+ +ND + SVEHRVLAN  +  
Sbjct: 249 TILLQDHIGGLQVLHDQYWVDVPPVPGALVVNVGDLLQLITNDKFISVEHRVLAN-VAGP 308

Query: 337 RVSVAVFY------NPRIRDSLYGPLPELISQDTPAVYRHFTYDDYITRFFNKELDGKS 389
           R+SVA F+      NPR+    YGP+ E++S++ P  YR  T  +Y   + +K  DG S
Sbjct: 309 RISVACFFSSYLMANPRV----YGPIKEILSEENPPNYRDTTITEYAKFYRSKGFDGTS 358

BLAST of Spg028742 vs. TAIR 10
Match: AT1G04350.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 334.7 bits (857), Expect = 9.9e-92
Identity = 172/361 (47.65%), Postives = 233/361 (64.54%), Query Frame = 0

Query: 38  DFDRATELKQFDQTKAGVKGLLDAGLKQIPRIFIHPPETLSDLKPTLKSPSSAAVVVPTI 97
           +FD  +E K FD+TK GVKGL+DA + +IPRIF  P  +LSD KP +   S+    +P I
Sbjct: 5   EFDSYSERKAFDETKTGVKGLIDAHITEIPRIFCLPQGSLSDKKPFV---STTDFAIPII 64

Query: 98  DLAGIHSPDLRPSIVDQIRNAASTFGFFQIINHGVPQKVLDEILDSVRAFHELPSEAKSP 157
           D  G+H    R  IV +I++AAS +GFFQ+INHGVP  VL EI D VR FHE   E K  
Sbjct: 65  DFEGLHVS--REDIVGKIKDAASNWGFFQVINHGVPLNVLQEIQDGVRRFHEEAPEVKKT 124

Query: 158 YYRRDVGTGVSYLSNIDLFNAKA-ASWRDTIQIGLGPVAAATENIPETCRAAVAEWAVET 217
           Y+ RD      Y SN DL+++ +  +WRD+    + P     E++P  CR A+ E++   
Sbjct: 125 YFTRDATKRFVYNSNFDLYSSSSCVNWRDSFACYMAPDPPNPEDLPVACRVAMFEYSKHM 184

Query: 218 ERLGYLLEEVLCEGLGLKSERLKEATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDPGVLT 277
            RLG LL E+L E LGL+S++LK     +G +++ HYYP CPQPDLT+G  +H+D   LT
Sbjct: 185 MRLGDLLFELLSEALGLRSDKLKSMDCMKGLLLLCHYYPPCPQPDLTIGTNNHSDNSFLT 244

Query: 278 VLLQDHIGGLQIRHGGGWVDVEPVAGALVINIGDLLQIASNDVYKSVEHRVLANGKSEAR 337
           +LLQD IGGLQI H   WVDV P+ GALVIN+GD LQ+ +ND   SVEHRVLAN  +  R
Sbjct: 245 ILLQDQIGGLQIFHQDCWVDVSPIPGALVINMGDFLQLITNDKVISVEHRVLANRAATPR 304

Query: 338 VSVAVFYNPRIR--DSLYGPLPELISQDTPAVYRHFTYDDYITRFFNKELDGKSLPNHYR 396
           +SVA F++  +R   ++YGP+ EL+S++ P+ YR     +Y   +F K LDG S  +HY+
Sbjct: 305 ISVASFFSTSMRPNSTVYGPIKELLSEENPSKYRVIDLKEYTEGYFKKGLDGTSYLSHYK 360

BLAST of Spg028742 vs. TAIR 10
Match: AT2G30840.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 332.8 bits (852), Expect = 3.8e-91
Identity = 168/365 (46.03%), Postives = 233/365 (63.84%), Query Frame = 0

Query: 35  MAADFDRATELKQFDQTKAGVKGLLDAGLKQIPRIFIHPPETLSDLKPTLKSPSSAAVVV 94
           M A +DRA+E+K FD+ K GVKGLLDAG+ QIPRIF HP   L+D    L   SS  +V+
Sbjct: 1   MEATYDRASEVKAFDELKIGVKGLLDAGVTQIPRIFHHPHLNLTDSNLLL---SSTTMVI 60

Query: 95  PTIDLAGIHSPD---LRPSIVDQIRNAASTFGFFQIINHGVPQKVLDEILDSVRAFHELP 154
           PTIDL G    +    R S++  IR+A   FGFFQ+INHG+   V++++ D +R FHE  
Sbjct: 61  PTIDLKGGVFDEYTVTRESVIAMIRDAVERFGFFQVINHGISNDVMEKMKDGIRGFHEQD 120

Query: 155 SEAKSPYYRRDVGTGVSYLSNIDLFNAKAASWRDTIQIGLGPVAAATENIPETCRAAVAE 214
           S+ +  +Y RDV   V Y SN DL+++ +A+WRDT+   + P    TE++P+ C   + E
Sbjct: 121 SDVRKKFYTRDVTKTVKYNSNFDLYSSPSANWRDTLSCFMAPDVPETEDLPDICGEIMLE 180

Query: 215 WAVETERLGYLLEEVLCEGLGLKSERLKEATYGEGRMMVGHYYPYCPQPDLTVGIKSHTD 274
           +A    +LG L+ E+L E LGL    LKE    +G +M+ HYYP CP+P LT G   H+D
Sbjct: 181 YAKRVMKLGELIFELLSEALGLNPNHLKEMDCTKGLLMLSHYYPPCPEPGLTFGTSPHSD 240

Query: 275 PGVLTVLLQDHIGGLQIRHGGGWVDVEPVAGALVINIGDLLQIASNDVYKSVEHRVLANG 334
              LT+LLQDHIGGLQ+R  G WVDV PV GAL++N+GDLLQ+ +ND + SVEHRVLAN 
Sbjct: 241 RSFLTILLQDHIGGLQVRQNGYWVDVPPVPGALLVNLGDLLQLMTNDQFVSVEHRVLANK 300

Query: 335 KSEARVSVAVFY-NPRIRDSLYGPLPELISQDTPAVYRHFTYDDYITRFFNKELDGKSLP 394
             + R+SVA F+ +P     +YGP+ EL+S+     YR  T  +Y + +  + L G S+ 
Sbjct: 301 GEKPRISVASFFVHPLPSLRVYGPIKELLSEQNLPKYRDTTVTEYTSHYMARGLYGNSVL 360

Query: 395 NHYRI 396
             ++I
Sbjct: 361 LDFKI 362

BLAST of Spg028742 vs. TAIR 10
Match: AT2G30830.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 332.8 bits (852), Expect = 3.8e-91
Identity = 162/364 (44.51%), Postives = 232/364 (63.74%), Query Frame = 0

Query: 35  MAADFDRATELKQFDQTKAGVKGLLDAGLKQIPRIFIHPPETLSDLKPTLKSPSSAAVVV 94
           MA ++DRA E+K FDQ K GVKGL+DAG+ ++PRIF H    +++ KP      S+ + +
Sbjct: 1   MAGNYDRAGEVKAFDQMKIGVKGLVDAGITKVPRIFHHQDVAVTNPKP------SSTLEI 60

Query: 95  PTIDLAG--IHSPDLRPSIVDQIRNAASTFGFFQIINHGVPQKVLDEILDSVRAFHELPS 154
           PTID+ G    S   R S++ ++R A   FGFFQ+INHG+P +V++ + D +R FHE  S
Sbjct: 61  PTIDVGGGVFESTVTRKSVIAKVRAAVEKFGFFQVINHGIPLEVMESMKDGIRGFHEQDS 120

Query: 155 EAKSPYYRRDVGTGVSYLSNIDLFNAKAASWRDTIQIGLGPVAAATENIPETCRAAVAEW 214
           E K  +Y RD+   V Y +N DL++++AA+WRDT+ + + P      ++P  CR  + E+
Sbjct: 121 EVKKTFYSRDITKKVKYNTNFDLYSSQAANWRDTLTMVMAPDVPQAGDLPVICREIMLEY 180

Query: 215 AVETERLGYLLEEVLCEGLGLKSERLKEATYGEGRMMVGHYYPYCPQPDLTVGIKSHTDP 274
           +    +LG L+ E+L E LGLK   LKE    +   ++ HYYP CP+PD T GI SHTD 
Sbjct: 181 SKRMMKLGELIFELLSEALGLKPNHLKELNCAKSLSLLSHYYPPCPEPDRTFGISSHTDI 240

Query: 275 GVLTVLLQDHIGGLQIRHGGGWVDVEPVAGALVINIGDLLQIASNDVYKSVEHRVLANGK 334
             +T+LLQDHIGGLQ+ H G W+DV P   AL++N+GDLLQ+ +ND + SVEHRVLAN  
Sbjct: 241 SFITILLQDHIGGLQVLHDGYWIDVPPNPEALIVNLGDLLQLITNDKFVSVEHRVLANRG 300

Query: 335 SEARVSVAVFYNPRI-RDSLYGPLPELISQDTPAVYRHFTYDDYITRFFNKELDGKSLPN 394
            E R+S A F+   I  + +YGP+ EL+S+  P  YR+ T  +    +  + LDG S   
Sbjct: 301 EEPRISSASFFMHTIPNEQVYGPMKELLSKQNPPKYRNTTTTEMARHYLARGLDGTSPLL 358

Query: 395 HYRI 396
           H+RI
Sbjct: 361 HFRI 358

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038892530.13.9e-20789.421-aminocyclopropane-1-carboxylate oxidase homolog 3-like [Benincasa hispida][more]
XP_022986647.19.9e-20387.661-aminocyclopropane-1-carboxylate oxidase homolog 3-like [Cucurbita maxima][more]
XP_008464717.11.6e-20087.63PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 [... [more]
XP_022943220.12.1e-20086.401-aminocyclopropane-1-carboxylate oxidase homolog 3-like [Cucurbita moschata][more]
XP_004142373.12.7e-20087.881-aminocyclopropane-1-carboxylate oxidase homolog 6 isoform X1 [Cucumis sativus]... [more]
Match NameE-valueIdentityDescription
Q8H1S42.8e-9146.741-aminocyclopropane-1-carboxylate oxidase homolog 3 OS=Arabidopsis thaliana OX=3... [more]
Q84MB36.3e-9147.911-aminocyclopropane-1-carboxylate oxidase homolog 1 OS=Arabidopsis thaliana OX=3... [more]
P938241.4e-9047.651-aminocyclopropane-1-carboxylate oxidase homolog 6 OS=Arabidopsis thaliana OX=3... [more]
Q9LTH83.8e-8847.571-aminocyclopropane-1-carboxylate oxidase homolog 11 OS=Arabidopsis thaliana OX=... [more]
Q9LTH73.2e-8746.071-aminocyclopropane-1-carboxylate oxidase homolog 12 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1J8544.8e-20387.661-aminocyclopropane-1-carboxylate oxidase homolog 3-like OS=Cucurbita maxima OX=... [more]
A0A1S3CML87.7e-20187.631-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 OS=Cucumis m... [more]
A0A6J1FXJ91.0e-20086.401-aminocyclopropane-1-carboxylate oxidase homolog 3-like OS=Cucurbita moschata O... [more]
A0A0A0KV771.3e-20087.88Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
A0A1S4E5J84.2e-19986.751-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X1 OS=Cucumis m... [more]
Match NameE-valueIdentityDescription
AT1G06650.22.0e-9246.742-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G06620.14.4e-9247.912-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G04350.19.9e-9247.652-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G30840.13.8e-9146.032-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G30830.13.8e-9144.512-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 248..345
e-value: 6.6E-26
score: 90.7
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 94..191
e-value: 9.0E-19
score: 68.4
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 45..377
e-value: 1.8E-99
score: 335.4
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 36..381
NoneNo IPR availablePANTHERPTHR10209:SF5461-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE HOMOLOG 4-LIKEcoord: 36..381
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 52..387
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 244..345
score: 13.944705

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg028742.1Spg028742.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity