Spg028734 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg028734
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCarboxypeptidase
Locationscaffold7: 15530169 .. 15531554 (-)
RNA-Seq ExpressionSpg028734
SyntenySpg028734
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTCATCTAACTTCCTTCTCTCCATCTTCTTTTCCTTCTTCTCCTTCTTCTTCTTCGCTTCATTTCCATTTCCAGCCACTTCATCTCCGCCATTTTTTCCGGCCGGAGCTCTTCCGACCAAATCAGGCTATCTCCCAATCAACCCCGCCTCCAGCTCCGCTATTTTCTTCGCCTTCTACGAAGCCCAAGCCCCAGTTTCCCCTCTCTCAAAAACCCCTCTTCTAATCTGGCTCCAAGGCGGCCCTGGCTGTTCCTCCATGACCGGCAACTTCTTCGAGCTCGGTCCATGGCGGGTCAACTTCCACAAAGAAAAAACCGACCCCATCTCCCTAATCCCCAATCCAGGCTCGTGGAACCGAAGATTCGGGGTTCTATTTCTCGACAACCCAATTGGAACCGGCTTCAGCATTGCAGCAACAGAAGATGAGATCCCTAGAGACCAATACTCTGTTTCTAAGCATCTTTTTCCTGCAATTTCTTCGTTTATTGAGCTGGATTTGGCGTTTAAGAGTCGACCCATTTACATTACAGGGGAGAGCTATGCAGGGAAATATGTGCCTTCAATTGGGTATTACATTTTGAAGAAAAACCCCAAATTGCCTGTCGGTAAGAGGCTCAATCTGGCTGGTGTTGCCATTGGAGATGGGCTGACAGACCCTATTACTCAAGTTGCCACTCATGGGCTGAATGCTTTCTATTCTGGGTTGATCAATGAGAAGCAAAGAAAAGAATTGGAGGTTGCTCAAATGGAGGCTGTGGAGTTGACTAAAATGGGGAATTGGAGTGAGGCCACAAATGCAAGGATTAGGGTCTTGGAATTGCTCAAGAACATGACTGGATTGGCCACTTTGTATGATTATACAAGGAAAGCTCCTTACAATTCAGATTTGGTGGCTGAGTTTTTGAGCTCCAAGGAGGTGAAGAGGGCTTTGGGAGTGAATGAGTCAATGGTTTATGAAGAGTGTAGTGATCTTGTTGGGGAGATTTTGAGTGATGATGTGATGAAGAGTGTGAAGTATAAGGTTGATTTTTTGGTGAAGAATAGTAAGGTTTTACTTTATCAAGGGCAGTATGATTTGAGGGATGGTGTGGTTTCAACTGAGGCTTGGGTGAAGAAGTTGAAATGGGAAGAGATTGAGAGGTTTTTGGAAGCAGATAGAAAGATTTGGAGAGTTGGAGATGAGTTAGCTGGTTATGTTCAGAAATGGAAGAGTTTGACCAATGTTGTTGTGTTGGGAGCTGGTCATCTTCTTCCCACTGATCAGGCTTTGAATTCTCAAGCAATGATTGAAGATTGGGTTTTGGAGAAAGGAGTTTTTGGTGATGATAAAGCACAAAAGAAGAAGCCTTTTGCTACTACTGGTTTTGGGGTTTGGCTATGA

mRNA sequence

ATGGACTCATCTAACTTCCTTCTCTCCATCTTCTTTTCCTTCTTCTCCTTCTTCTTCTTCGCTTCATTTCCATTTCCAGCCACTTCATCTCCGCCATTTTTTCCGGCCGGAGCTCTTCCGACCAAATCAGGCTATCTCCCAATCAACCCCGCCTCCAGCTCCGCTATTTTCTTCGCCTTCTACGAAGCCCAAGCCCCAGTTTCCCCTCTCTCAAAAACCCCTCTTCTAATCTGGCTCCAAGGCGGCCCTGGCTGTTCCTCCATGACCGGCAACTTCTTCGAGCTCGGTCCATGGCGGGTCAACTTCCACAAAGAAAAAACCGACCCCATCTCCCTAATCCCCAATCCAGGCTCGTGGAACCGAAGATTCGGGGTTCTATTTCTCGACAACCCAATTGGAACCGGCTTCAGCATTGCAGCAACAGAAGATGAGATCCCTAGAGACCAATACTCTGTTTCTAAGCATCTTTTTCCTGCAATTTCTTCGTTTATTGAGCTGGATTTGGCGTTTAAGAGTCGACCCATTTACATTACAGGGGAGAGCTATGCAGGGAAATATGTGCCTTCAATTGGGTATTACATTTTGAAGAAAAACCCCAAATTGCCTGTCGGTAAGAGGCTCAATCTGGCTGGTGTTGCCATTGGAGATGGGCTGACAGACCCTATTACTCAAGTTGCCACTCATGGGCTGAATGCTTTCTATTCTGGGTTGATCAATGAGAAGCAAAGAAAAGAATTGGAGGTTGCTCAAATGGAGGCTGTGGAGTTGACTAAAATGGGGAATTGGAGTGAGGCCACAAATGCAAGGATTAGGGTCTTGGAATTGCTCAAGAACATGACTGGATTGGCCACTTTGTATGATTATACAAGGAAAGCTCCTTACAATTCAGATTTGGTGGCTGAGTTTTTGAGCTCCAAGGAGGTGAAGAGGGCTTTGGGAGTGAATGAGTCAATGGTTTATGAAGAGTGTAGTGATCTTGTTGGGGAGATTTTGAGTGATGATGTGATGAAGAGTGTGAAGTATAAGGTTGATTTTTTGGTGAAGAATAGTAAGGTTTTACTTTATCAAGGGCAGTATGATTTGAGGGATGGTGTGGTTTCAACTGAGGCTTGGGTGAAGAAGTTGAAATGGGAAGAGATTGAGAGGTTTTTGGAAGCAGATAGAAAGATTTGGAGAGTTGGAGATGAGTTAGCTGGTTATGTTCAGAAATGGAAGAGTTTGACCAATGTTGTTGTGTTGGGAGCTGGTCATCTTCTTCCCACTGATCAGGCTTTGAATTCTCAAGCAATGATTGAAGATTGGGTTTTGGAGAAAGGAGTTTTTGGTGATGATAAAGCACAAAAGAAGAAGCCTTTTGCTACTACTGGTTTTGGGGTTTGGCTATGA

Coding sequence (CDS)

ATGGACTCATCTAACTTCCTTCTCTCCATCTTCTTTTCCTTCTTCTCCTTCTTCTTCTTCGCTTCATTTCCATTTCCAGCCACTTCATCTCCGCCATTTTTTCCGGCCGGAGCTCTTCCGACCAAATCAGGCTATCTCCCAATCAACCCCGCCTCCAGCTCCGCTATTTTCTTCGCCTTCTACGAAGCCCAAGCCCCAGTTTCCCCTCTCTCAAAAACCCCTCTTCTAATCTGGCTCCAAGGCGGCCCTGGCTGTTCCTCCATGACCGGCAACTTCTTCGAGCTCGGTCCATGGCGGGTCAACTTCCACAAAGAAAAAACCGACCCCATCTCCCTAATCCCCAATCCAGGCTCGTGGAACCGAAGATTCGGGGTTCTATTTCTCGACAACCCAATTGGAACCGGCTTCAGCATTGCAGCAACAGAAGATGAGATCCCTAGAGACCAATACTCTGTTTCTAAGCATCTTTTTCCTGCAATTTCTTCGTTTATTGAGCTGGATTTGGCGTTTAAGAGTCGACCCATTTACATTACAGGGGAGAGCTATGCAGGGAAATATGTGCCTTCAATTGGGTATTACATTTTGAAGAAAAACCCCAAATTGCCTGTCGGTAAGAGGCTCAATCTGGCTGGTGTTGCCATTGGAGATGGGCTGACAGACCCTATTACTCAAGTTGCCACTCATGGGCTGAATGCTTTCTATTCTGGGTTGATCAATGAGAAGCAAAGAAAAGAATTGGAGGTTGCTCAAATGGAGGCTGTGGAGTTGACTAAAATGGGGAATTGGAGTGAGGCCACAAATGCAAGGATTAGGGTCTTGGAATTGCTCAAGAACATGACTGGATTGGCCACTTTGTATGATTATACAAGGAAAGCTCCTTACAATTCAGATTTGGTGGCTGAGTTTTTGAGCTCCAAGGAGGTGAAGAGGGCTTTGGGAGTGAATGAGTCAATGGTTTATGAAGAGTGTAGTGATCTTGTTGGGGAGATTTTGAGTGATGATGTGATGAAGAGTGTGAAGTATAAGGTTGATTTTTTGGTGAAGAATAGTAAGGTTTTACTTTATCAAGGGCAGTATGATTTGAGGGATGGTGTGGTTTCAACTGAGGCTTGGGTGAAGAAGTTGAAATGGGAAGAGATTGAGAGGTTTTTGGAAGCAGATAGAAAGATTTGGAGAGTTGGAGATGAGTTAGCTGGTTATGTTCAGAAATGGAAGAGTTTGACCAATGTTGTTGTGTTGGGAGCTGGTCATCTTCTTCCCACTGATCAGGCTTTGAATTCTCAAGCAATGATTGAAGATTGGGTTTTGGAGAAAGGAGTTTTTGGTGATGATAAAGCACAAAAGAAGAAGCCTTTTGCTACTACTGGTTTTGGGGTTTGGCTATGA

Protein sequence

MDSSNFLLSIFFSFFSFFFFASFPFPATSSPPFFPAGALPTKSGYLPINPASSSAIFFAFYEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFFELGPWRVNFHKEKTDPISLIPNPGSWNRRFGVLFLDNPIGTGFSIAATEDEIPRDQYSVSKHLFPAISSFIELDLAFKSRPIYITGESYAGKYVPSIGYYILKKNPKLPVGKRLNLAGVAIGDGLTDPITQVATHGLNAFYSGLINEKQRKELEVAQMEAVELTKMGNWSEATNARIRVLELLKNMTGLATLYDYTRKAPYNSDLVAEFLSSKEVKRALGVNESMVYEECSDLVGEILSDDVMKSVKYKVDFLVKNSKVLLYQGQYDLRDGVVSTEAWVKKLKWEEIERFLEADRKIWRVGDELAGYVQKWKSLTNVVVLGAGHLLPTDQALNSQAMIEDWVLEKGVFGDDKAQKKKPFATTGFGVWL
Homology
BLAST of Spg028734 vs. NCBI nr
Match: XP_022977756.1 (serine carboxypeptidase-like 50 [Cucurbita maxima])

HSP 1 Score: 799.3 bits (2063), Expect = 1.8e-227
Identity = 387/460 (84.13%), Postives = 427/460 (92.83%), Query Frame = 0

Query: 1   MDSSNFLLSIFFSFFSFFFFASFPFPATSSPPFFPAGALPTKSGYLPINPASSSAIFFAF 60
           MDS+N LLS+FF    F FFASFP PATSSPPFFPA ALPTKSGYLPINP + SAI+FAF
Sbjct: 1   MDSTNVLLSVFF----FLFFASFPVPATSSPPFFPAEALPTKSGYLPINPTTGSAIYFAF 60

Query: 61  YEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFFELGPWRVNFHKEKTDPISLIPNPGSWN 120
           YEAQ P+S +SKTPLLIWLQGGPGCSSM GNFFELGPWRVNFHK++TDP+SLIPN GSWN
Sbjct: 61  YEAQTPISSISKTPLLIWLQGGPGCSSMIGNFFELGPWRVNFHKKETDPVSLIPNTGSWN 120

Query: 121 RRFGVLFLDNPIGTGFSIAATEDEIPRDQYSVSKHLFPAISSFIELDLAFKSRPIYITGE 180
           RRFGVLFLDNPIGTGFSIAAT+DEIP++QYSVSK+LF AISSFIE D+ FK+RPIYITGE
Sbjct: 121 RRFGVLFLDNPIGTGFSIAATKDEIPKNQYSVSKYLFSAISSFIEQDITFKNRPIYITGE 180

Query: 181 SYAGKYVPSIGYYILKKNPKLPVGKRLNLAGVAIGDGLTDPITQVATHGLNAFYSGLINE 240
           SYAGKYVPSIGYYILKKNPKLPV K+LNLAGVAIG+GLTDPITQVATH LNA+YSGLINE
Sbjct: 181 SYAGKYVPSIGYYILKKNPKLPVDKKLNLAGVAIGNGLTDPITQVATHALNAYYSGLINE 240

Query: 241 KQRKELEVAQMEAVELTKMGNWSEATNARIRVLELLKNMTGLATLYDYTRKAPYNSDLVA 300
           +Q+KE+E+AQ+EAVELTK GNWSEATNAR +VL LLKNMTGLATLYDYTRK+PYNS+LVA
Sbjct: 241 RQKKEMEIAQLEAVELTKKGNWSEATNARSKVLNLLKNMTGLATLYDYTRKSPYNSELVA 300

Query: 301 EFLSSKEVKRALGVNESMVYEECSDLVGEILSDDVMKSVKYKVDFLVKNSKVLLYQGQYD 360
           EFLS KEVKRALGVNESMV+EECSDLVGE+L DDVMKS++YKVD+LVKNSKVL+YQGQ+D
Sbjct: 301 EFLSFKEVKRALGVNESMVFEECSDLVGEVLKDDVMKSMRYKVDYLVKNSKVLVYQGQFD 360

Query: 361 LRDGVVSTEAWVKKLKWEEIERFLEADRKIWRVGDELAGYVQKWKSLTNVVVLGAGHLLP 420
           LRDGVVSTEAW+KK+KW EIERFL ADRKIW VGDELAGYVQKWKSL+N VVLGAGHL+P
Sbjct: 361 LRDGVVSTEAWLKKVKWGEIERFLGADRKIWSVGDELAGYVQKWKSLSNAVVLGAGHLVP 420

Query: 421 TDQALNSQAMIEDWVLEKGVFGDDKAQKKKPFATTGFGVW 461
           +DQALNSQAMIEDWVLEKGVFGDD A++KKPF    FGVW
Sbjct: 421 SDQALNSQAMIEDWVLEKGVFGDDNAEEKKPFGANDFGVW 456

BLAST of Spg028734 vs. NCBI nr
Match: XP_038893258.1 (serine carboxypeptidase-like 50 [Benincasa hispida] >XP_038893259.1 serine carboxypeptidase-like 50 [Benincasa hispida])

HSP 1 Score: 797.7 bits (2059), Expect = 5.2e-227
Identity = 394/462 (85.28%), Postives = 425/462 (91.99%), Query Frame = 0

Query: 1   MDSSNFLLSIFFSFFSFFFFASFPFPATSSPPFFPAGALPTKSGYLPINPASSSAIFFAF 60
           M+S NFLLS+FF FF FFFFASFPFP+TSSPP FP  ALPTKSGYLPINP + SAIFF F
Sbjct: 1   MNSINFLLSVFF-FFFFFFFASFPFPSTSSPPLFPPEALPTKSGYLPINPTTGSAIFFTF 60

Query: 61  YEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFFELGPWRVNFHKEKTDPISLIPNPGSWN 120
           YEAQ P+S L+KTPLLIWLQGGPGCSSM GNFFELGPWRVNFHK+KT+PISLIPNPGSWN
Sbjct: 61  YEAQTPISSLTKTPLLIWLQGGPGCSSMVGNFFELGPWRVNFHKQKTEPISLIPNPGSWN 120

Query: 121 RRFGVLFLDNPIGTGFSIAATEDEIPRDQYSVSKHLFPAISSFIELDLAFKSRPIYITGE 180
           RRFGVLFLDNPIGTGFSIAAT+DEIPR+QYSVSKHLF AISSFIELDL FK+RPIYITGE
Sbjct: 121 RRFGVLFLDNPIGTGFSIAATQDEIPRNQYSVSKHLFTAISSFIELDLVFKNRPIYITGE 180

Query: 181 SYAGKYVPSIGYYILKKNPKLPVGKRLNLAGVAIGDGLTDPITQVATHGLNAFYSGLINE 240
           SYAGKYVPSIGYYILKKNPKLP  KR+NL GVAIGDGLTDPITQV THGLNAFYSGLINE
Sbjct: 181 SYAGKYVPSIGYYILKKNPKLPYDKRVNLVGVAIGDGLTDPITQVTTHGLNAFYSGLINE 240

Query: 241 KQRKELEVAQMEAVELTKMGNWSEATNARIRVLELLKNMTGLATLYDYTRKAPYNSDLVA 300
           KQ+KE+E+AQM+AVEL KMG W+EATNAR++VL+LLK+MTGLATLYDYTRK+ YN+DLV 
Sbjct: 241 KQKKEMEIAQMKAVELIKMGKWNEATNARLKVLDLLKDMTGLATLYDYTRKSAYNTDLVD 300

Query: 301 EFLSSKEVKRALGV-NESMVYEECSDLVGEILSDDVMKSVKYKVDFLVKNSKVLLYQGQY 360
           EFLS KEVKRALGV NESMV+E CSD+VGE+L DDVMKSV+YKVDFLVKNSKVLLYQGQY
Sbjct: 301 EFLSFKEVKRALGVKNESMVFEGCSDVVGEVLKDDVMKSVRYKVDFLVKNSKVLLYQGQY 360

Query: 361 DLRDGVVSTEAWVKKLKWEEIERFLEADRKIWRVGDELAGYVQKWKSLTNVVVLGAGHLL 420
           DLRDGVVSTEAWV KLKW+EIE FL  +RKIWRVGDELAGYVQKW SL+NVVVLGAGHLL
Sbjct: 361 DLRDGVVSTEAWVNKLKWDEIENFLGVERKIWRVGDELAGYVQKWMSLSNVVVLGAGHLL 420

Query: 421 PTDQALNSQAMIEDWVLEKGVFGDDKAQ-KKKPFATTGFGVW 461
           PTDQALNSQAMIEDWVLEKGVFGDD AQ KKK F + GF VW
Sbjct: 421 PTDQALNSQAMIEDWVLEKGVFGDDNAQEKKKSFGSNGFRVW 461

BLAST of Spg028734 vs. NCBI nr
Match: XP_023534635.1 (serine carboxypeptidase-like 50 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023534643.1 serine carboxypeptidase-like 50 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 796.2 bits (2055), Expect = 1.5e-226
Identity = 387/460 (84.13%), Postives = 425/460 (92.39%), Query Frame = 0

Query: 1   MDSSNFLLSIFFSFFSFFFFASFPFPATSSPPFFPAGALPTKSGYLPINPASSSAIFFAF 60
           MDS+N LLS+FF    F FFASFP  ATSSPPFFPA ALPTKSGYLPINP + SAI+FAF
Sbjct: 1   MDSTNVLLSVFF----FLFFASFPVLATSSPPFFPAEALPTKSGYLPINPTTGSAIYFAF 60

Query: 61  YEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFFELGPWRVNFHKEKTDPISLIPNPGSWN 120
           YEAQ P+S +SKTPLLIWLQGGPGCSSM GNFFELGPWRVNFHK++TDP+SLIPN GSWN
Sbjct: 61  YEAQTPISSISKTPLLIWLQGGPGCSSMIGNFFELGPWRVNFHKKETDPVSLIPNTGSWN 120

Query: 121 RRFGVLFLDNPIGTGFSIAATEDEIPRDQYSVSKHLFPAISSFIELDLAFKSRPIYITGE 180
           RRFGVLFLDNPIGTGFSIAAT+DEIP++QYSVSKHLF AISSFIE D+ FK+RPIYITGE
Sbjct: 121 RRFGVLFLDNPIGTGFSIAATKDEIPKNQYSVSKHLFSAISSFIEQDITFKNRPIYITGE 180

Query: 181 SYAGKYVPSIGYYILKKNPKLPVGKRLNLAGVAIGDGLTDPITQVATHGLNAFYSGLINE 240
           SYAGKYVPSIGYYILKKNPKLPV KRLNLAGVAIG+GLTDPITQVATH LNA+YSGLINE
Sbjct: 181 SYAGKYVPSIGYYILKKNPKLPVDKRLNLAGVAIGNGLTDPITQVATHALNAYYSGLINE 240

Query: 241 KQRKELEVAQMEAVELTKMGNWSEATNARIRVLELLKNMTGLATLYDYTRKAPYNSDLVA 300
           +Q+KELE+AQ+EAVELTK GNWSEATNAR +VL LLKNMTGLATLYDYTRK+PYNS+LVA
Sbjct: 241 RQKKELEIAQLEAVELTKKGNWSEATNARSKVLNLLKNMTGLATLYDYTRKSPYNSELVA 300

Query: 301 EFLSSKEVKRALGVNESMVYEECSDLVGEILSDDVMKSVKYKVDFLVKNSKVLLYQGQYD 360
           EFLS KEVKRALGVNESMV+EECSDLVGE+L DDVMKS++YKVD+LVKNSKVL+YQGQ+D
Sbjct: 301 EFLSFKEVKRALGVNESMVFEECSDLVGEVLKDDVMKSMRYKVDYLVKNSKVLVYQGQFD 360

Query: 361 LRDGVVSTEAWVKKLKWEEIERFLEADRKIWRVGDELAGYVQKWKSLTNVVVLGAGHLLP 420
           LRDGVVSTEAW+KK+KW EIERFL ADRKIW VGDELAGYVQ WKSL+N VVLGAGHL+P
Sbjct: 361 LRDGVVSTEAWLKKVKWGEIERFLGADRKIWSVGDELAGYVQTWKSLSNAVVLGAGHLVP 420

Query: 421 TDQALNSQAMIEDWVLEKGVFGDDKAQKKKPFATTGFGVW 461
           +DQALNSQAMIEDWVLEKG+FGDD A++KKPF    FGVW
Sbjct: 421 SDQALNSQAMIEDWVLEKGLFGDDNAEEKKPFGANDFGVW 456

BLAST of Spg028734 vs. NCBI nr
Match: KAG6600816.1 (Serine carboxypeptidase-like 50, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 795.8 bits (2054), Expect = 2.0e-226
Identity = 385/460 (83.70%), Postives = 426/460 (92.61%), Query Frame = 0

Query: 1   MDSSNFLLSIFFSFFSFFFFASFPFPATSSPPFFPAGALPTKSGYLPINPASSSAIFFAF 60
           MDS+N LLS+FF    F FFASFP  ATSSPPFFPA ALPTKSGYLPINP + SAI+FAF
Sbjct: 1   MDSTNVLLSVFF----FLFFASFPVLATSSPPFFPAEALPTKSGYLPINPTTGSAIYFAF 60

Query: 61  YEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFFELGPWRVNFHKEKTDPISLIPNPGSWN 120
           YEAQ P+S +SKTPLLIWLQGGPGCSSM GNFFELGPWRVNFHK++TDP+SLIPN GSWN
Sbjct: 61  YEAQTPISSISKTPLLIWLQGGPGCSSMIGNFFELGPWRVNFHKKETDPVSLIPNTGSWN 120

Query: 121 RRFGVLFLDNPIGTGFSIAATEDEIPRDQYSVSKHLFPAISSFIELDLAFKSRPIYITGE 180
           RRFGVLFLDNPIGTGFSIAAT+DEIP++QYSVSKHLF AISSFIE D+ FK+RPIYITGE
Sbjct: 121 RRFGVLFLDNPIGTGFSIAATKDEIPKNQYSVSKHLFSAISSFIEQDITFKNRPIYITGE 180

Query: 181 SYAGKYVPSIGYYILKKNPKLPVGKRLNLAGVAIGDGLTDPITQVATHGLNAFYSGLINE 240
           SYAGKYVPSIGYYILKKNPKLPV KRLNLAGVAIG+GLTDPITQVATH LNA+YSGLINE
Sbjct: 181 SYAGKYVPSIGYYILKKNPKLPVDKRLNLAGVAIGNGLTDPITQVATHALNAYYSGLINE 240

Query: 241 KQRKELEVAQMEAVELTKMGNWSEATNARIRVLELLKNMTGLATLYDYTRKAPYNSDLVA 300
           +Q+KE+E+AQ+EAVELTK GNWSEATNAR +VL LLKNMTG+ATLYDYTRK+PYNS+LVA
Sbjct: 241 RQKKEMEIAQLEAVELTKKGNWSEATNARSKVLNLLKNMTGIATLYDYTRKSPYNSELVA 300

Query: 301 EFLSSKEVKRALGVNESMVYEECSDLVGEILSDDVMKSVKYKVDFLVKNSKVLLYQGQYD 360
           EFLS KEVKRALGVNESMV+EECSDLVGE+L DDVMKS++YKVD+LVKNSKVL+YQGQ+D
Sbjct: 301 EFLSFKEVKRALGVNESMVFEECSDLVGEVLKDDVMKSMRYKVDYLVKNSKVLVYQGQFD 360

Query: 361 LRDGVVSTEAWVKKLKWEEIERFLEADRKIWRVGDELAGYVQKWKSLTNVVVLGAGHLLP 420
           LRDGVVSTEAW+KK+KW EIERFL ADRKIWRVGDELAGYVQ WKSL+N VVLGAGHL+P
Sbjct: 361 LRDGVVSTEAWLKKVKWGEIERFLGADRKIWRVGDELAGYVQTWKSLSNAVVLGAGHLVP 420

Query: 421 TDQALNSQAMIEDWVLEKGVFGDDKAQKKKPFATTGFGVW 461
           +DQALNSQAMIEDWVLEKGVFGDD +++K+PF    FGVW
Sbjct: 421 SDQALNSQAMIEDWVLEKGVFGDDNSEEKRPFGANDFGVW 456

BLAST of Spg028734 vs. NCBI nr
Match: XP_022941709.1 (serine carboxypeptidase-like 50 [Cucurbita moschata])

HSP 1 Score: 793.5 bits (2048), Expect = 9.7e-226
Identity = 385/460 (83.70%), Postives = 426/460 (92.61%), Query Frame = 0

Query: 1   MDSSNFLLSIFFSFFSFFFFASFPFPATSSPPFFPAGALPTKSGYLPINPASSSAIFFAF 60
           MDS+N LLS+FF    F FFASFP  ATSSPPFFPA ALPTKSGYLPINP++ SAI+FAF
Sbjct: 1   MDSTNVLLSVFF----FLFFASFPVLATSSPPFFPAEALPTKSGYLPINPSTGSAIYFAF 60

Query: 61  YEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFFELGPWRVNFHKEKTDPISLIPNPGSWN 120
           YEAQ P+S +SKTPLLIWLQGGPGCSSM GNFFELGPWRVNFHK++TDP+SLIPN GSWN
Sbjct: 61  YEAQTPISSISKTPLLIWLQGGPGCSSMIGNFFELGPWRVNFHKKETDPVSLIPNTGSWN 120

Query: 121 RRFGVLFLDNPIGTGFSIAATEDEIPRDQYSVSKHLFPAISSFIELDLAFKSRPIYITGE 180
           RRFGVLFLDNPIGTGFSIAAT+DEIP++QYSVSKHLF AISSFIE D+ FK+RPIYITGE
Sbjct: 121 RRFGVLFLDNPIGTGFSIAATKDEIPKNQYSVSKHLFSAISSFIEQDITFKNRPIYITGE 180

Query: 181 SYAGKYVPSIGYYILKKNPKLPVGKRLNLAGVAIGDGLTDPITQVATHGLNAFYSGLINE 240
           SYAGKYVPSIGYYILKKN KLPV KRLNLAGVAIG+GLTDPITQVATH LNA+YSGLINE
Sbjct: 181 SYAGKYVPSIGYYILKKNHKLPVDKRLNLAGVAIGNGLTDPITQVATHALNAYYSGLINE 240

Query: 241 KQRKELEVAQMEAVELTKMGNWSEATNARIRVLELLKNMTGLATLYDYTRKAPYNSDLVA 300
           +Q+KE+E+AQ+EAVELT+ GNWSEATNAR +VL LLKNMTGLATLYDYTRK+PYNS+LVA
Sbjct: 241 RQKKEMEIAQLEAVELTRKGNWSEATNARSKVLNLLKNMTGLATLYDYTRKSPYNSELVA 300

Query: 301 EFLSSKEVKRALGVNESMVYEECSDLVGEILSDDVMKSVKYKVDFLVKNSKVLLYQGQYD 360
           EFLS KEVKRALGVNESMV+EECSDLVGE+L DDVMKS++YKVD+LVKNSKVL+YQGQ+D
Sbjct: 301 EFLSFKEVKRALGVNESMVFEECSDLVGEVLKDDVMKSMRYKVDYLVKNSKVLVYQGQFD 360

Query: 361 LRDGVVSTEAWVKKLKWEEIERFLEADRKIWRVGDELAGYVQKWKSLTNVVVLGAGHLLP 420
           LRDGVVSTEAW+KK+KW EIERFL ADRKIWRVGDELAGYVQ WKSL+N VVLGAGHL+P
Sbjct: 361 LRDGVVSTEAWLKKVKWGEIERFLGADRKIWRVGDELAGYVQTWKSLSNAVVLGAGHLVP 420

Query: 421 TDQALNSQAMIEDWVLEKGVFGDDKAQKKKPFATTGFGVW 461
           +DQALNSQAMIEDWVLEKGVFGDD A++K+PF    FGVW
Sbjct: 421 SDQALNSQAMIEDWVLEKGVFGDDNAEEKRPFGANDFGVW 456

BLAST of Spg028734 vs. ExPASy Swiss-Prot
Match: Q9M9Q6 (Serine carboxypeptidase-like 50 OS=Arabidopsis thaliana OX=3702 GN=SCPL50 PE=2 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 7.2e-139
Identity = 239/413 (57.87%), Postives = 311/413 (75.30%), Query Frame = 0

Query: 31  PPFFPAGALPTKSGYLPINPASSSAIFFAFYEAQAPVSPLSKTPLLIWLQGGPGCSSMTG 90
           PP FP  ALPTKSGYLP+ PA  S++F+AFYEAQ P +PL  TPLL+WLQGGPGCSSM G
Sbjct: 25  PPLFPDEALPTKSGYLPVKPAPGSSMFYAFYEAQEPTTPLPDTPLLVWLQGGPGCSSMIG 84

Query: 91  NFFELGPWRVNFHKEKTDPISLIPNPGSWNRRFGVLFLDNPIGTGFSIAATEDEIPRDQY 150
           NF+ELGPWRV      TD   L  NPG+WNR FG+LF+DNPIG GFSIAA++ +IP +Q 
Sbjct: 85  NFYELGPWRV--VSRATD---LERNPGAWNRLFGLLFVDNPIGVGFSIAASQQDIPTNQR 144

Query: 151 SVSKHLFPAISSFIELDLAFKSRPIYITGESYAGKYVPSIGYYILKKNPKLPVGKRLNLA 210
            V++HL+ A+  F+E + +F++RP+Y TGESYAGKYVP+IGYYILK+ P      ++NL 
Sbjct: 145 QVAEHLYAALVEFLEQNPSFENRPVYFTGESYAGKYVPAIGYYILKEKP----NGKVNLK 204

Query: 211 GVAIGDGLTDPITQVATHGLNAFYSGLINEKQRKELEVAQMEAVELTKMGNWSEATNARI 270
           G+AIG+GLTDP+TQV TH +N +YSGL+N KQR EL+ AQ  +V L K   W EA +AR 
Sbjct: 205 GLAIGNGLTDPVTQVQTHAVNVYYSGLVNAKQRVELQKAQEISVALVKSQKWREAADART 264

Query: 271 RVLELLKNMTGLATLYDYTRKAPYNSDLVAEFLSSKEVKRALGVNESMVYEECSDLVGEI 330
            +L LL NMTGLATLY+  R  PY +DLV + L+ +E KR LGV+E++ +EECSD V ++
Sbjct: 265 ELLTLLSNMTGLATLYNTARAIPYRTDLVVDLLNQREAKRVLGVSETVRFEECSDEVEDV 324

Query: 331 LSDDVMKSVKYKVDFLVKNSKVLLYQGQYDLRDGVVSTEAWVKKLKWEEIERFLEADRKI 390
           L  DVMKSVK+ V++ ++ ++VLLYQG  DLRDGVVSTE W+K + W  +  F  A+R++
Sbjct: 325 LRADVMKSVKFMVEYALERTQVLLYQGMLDLRDGVVSTEEWMKTMNWSGLGMFSTAERRV 384

Query: 391 WRVGD-ELAGYVQKWKSLTNVVVLGAGHLLPTDQALNSQAMIEDWVLEKGVFG 443
           W+  D  +AGYVQ+W +L +V V GAGH +PTD+A+NS+ MIE WVL KG+FG
Sbjct: 385 WKDEDGVVAGYVQRWGNLCHVAVTGAGHFVPTDKAVNSRDMIEGWVLGKGLFG 428

BLAST of Spg028734 vs. ExPASy Swiss-Prot
Match: Q869Q8 (Probable serine carboxypeptidase CPVL OS=Dictyostelium discoideum OX=44689 GN=cpvl PE=3 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.9e-62
Identity = 139/404 (34.41%), Postives = 229/404 (56.68%), Query Frame = 0

Query: 41  TKSGYLPINPASSSAIFFAFYEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFFELGPWRV 100
           T +G++ +N   +S  FF F E+Q   +    +PL+I+LQGGPG +S    F E GP+  
Sbjct: 102 TFTGFITVNETYNSNTFFWFLESQ---NGDKNSPLVIFLQGGPGGASTFSLFVETGPY-- 161

Query: 101 NFHKEKTDPISLIPNPGSWNRRFGVLFLDNPIGTGFSIAATEDEIPRDQYSVSKHLFPAI 160
               E  D  +L+    +WN  F +L++DNP+GTGFS   +++    ++  ++ +L+  +
Sbjct: 162 ----ELLDNFTLVQREITWNSEFAMLYIDNPVGTGFSFTDSQEGYSNNEDEIATNLYTFL 221

Query: 161 SSFIELDLAFKSRPIYITGESYAGKYVPSIGYYILKKNPKLPVGKRLNLAGVAIGDGLTD 220
             F +L   + +  +YITGESYAGKY+P+  Y+I+++N        +NL G+AIGDGL D
Sbjct: 222 QQFYKLYPEYYTNELYITGESYAGKYIPAFSYHIIQQNQN-SNNPNINLKGIAIGDGLCD 281

Query: 221 PITQVATHGLNAFYSGLINEKQRKELEVAQMEAVELTKMGNWSEATNARIRVL----ELL 280
           PITQV  +   AFY+GL + +Q++ +   Q + VE      WS A +    ++    +  
Sbjct: 282 PITQVTQYANLAFYTGLADLQQQEVMFEYQDKIVEAINQEQWSVANDLFTDLINGPPDYF 341

Query: 281 KNMTGLATLYDY--TRKAPYNSDLVAEFLSSKEVKRALGVNESMVYEECSDLVGEILSDD 340
           +N+TG +  YD   T +  Y  D  A FL+   ++  + V  +  Y + ++ V   L  D
Sbjct: 342 QNITGESDYYDIRKTVEPTYGGDFTA-FLNQSSIRAMIHVGNN--YFQNNNDVYIHLEQD 401

Query: 341 VMKSVKYKVDFLVKNSKVLLYQGQYDLRDGVVSTEAWVKKLKWEEIERFLEADRKIWRVG 400
           + KSVK     ++ N KV+LY GQ+D   G   TE  ++ ++WE I+ FLE+ + IW++ 
Sbjct: 402 IPKSVKQLFPTILDNIKVILYNGQFDFIVGPSLTETMIRTIEWEGIQPFLESPKIIWKIP 461

Query: 401 D---ELAGYVQKWKSLTNVVVLGAGHLLPTDQALNSQAMIEDWV 436
               ++AG+V++W S T VVV  AGH++P DQ   +  MI+ ++
Sbjct: 462 SDNVDVAGFVRQWNSFTQVVVRQAGHMVPLDQPARAFDMIDRFI 492

BLAST of Spg028734 vs. ExPASy Swiss-Prot
Match: Q9H3G5 (Probable serine carboxypeptidase CPVL OS=Homo sapiens OX=9606 GN=CPVL PE=1 SV=2)

HSP 1 Score: 232.3 bits (591), Expect = 1.1e-59
Identity = 141/425 (33.18%), Postives = 229/425 (53.88%), Query Frame = 0

Query: 34  FPAGALPTKSGYLPINPASSSAIFFAFYEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFF 93
           FP   + + +G+L +N   +S +FF F+ AQ  + P    P+++WLQGGPG SSM G F 
Sbjct: 66  FPGLNMKSYAGFLTVNKTYNSNLFFWFFPAQ--IQP-EDAPVVLWLQGGPGGSSMFGLFV 125

Query: 94  ELGPWRVNFHKEKTDPISLIPNPGSWNRRFGVLFLDNPIGTGFSI-------AATEDEIP 153
           E GP+ V      T  ++L      W     +L++DNP+GTGFS        A  ED++ 
Sbjct: 126 EHGPYVV------TSNMTLRDRDFPWTTTLSMLYIDNPVGTGFSFTDDTHGYAVNEDDVA 185

Query: 154 RDQYSVSKHLFPAISSFIELDLAFKSRPIYITGESYAGKYVPSIGYYILKKNPKLPVGKR 213
           RD YS       A+  F ++   +K+   Y+TGESYAGKYVP+I + I   NP   V  +
Sbjct: 186 RDLYS-------ALIQFFQIFPEYKNNDFYVTGESYAGKYVPAIAHLIHSLNPVREV--K 245

Query: 214 LNLAGVAIGDGLTDPITQVATHGLNAFYSGLINEKQRKELEVAQMEAVELTKMGNWSEAT 273
           +NL G+AIGDG +DP + +  +    +  GL++EKQ+K  +    E +E  +  NW EA 
Sbjct: 246 INLNGIAIGDGYSDPESIIGGYAEFLYQIGLLDEKQKKYFQKQCHECIEHIRKQNWFEAF 305

Query: 274 NARIRVLE--------LLKNMTGLATLYDYTR-KAPYNSDLVAEFLSSKEVKRALGVNES 333
               ++L+          +N+TG +  Y++ R   P +     +FLS  EV++A+ V   
Sbjct: 306 EILDKLLDGDLTSDPSYFQNVTGCSNYYNFLRCTEPEDQLYYVKFLSLPEVRQAIHVGNQ 365

Query: 334 MVYEECSDLVGEILSDDVMKSVKYKVDFLVKNSKVLLYQGQYDLRDGVVSTEAWVKKLKW 393
              +    +V + L +D ++SVK  +  ++ N KVL+Y GQ D+      TE  +  + W
Sbjct: 366 TFND--GTIVEKYLREDTVQSVKPWLTEIMNNYKVLIYNGQLDIIVAAALTERSLMGMDW 425

Query: 394 EEIERFLEADRKIWRV---GDELAGYVQKWKSLTNVVVLGAGHLLPTDQALNSQAMIEDW 440
           +  + + +A++K+W++     E+AGY+++      V++ G GH+LP DQ L +  MI  +
Sbjct: 426 KGSQEYKKAEKKVWKIFKSDSEVAGYIRQAGDFHQVIIRGGGHILPYDQPLRAFDMINRF 470

BLAST of Spg028734 vs. ExPASy Swiss-Prot
Match: Q54DY7 (Serine carboxypeptidase S10 family member 1 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0291912 PE=3 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.9e-59
Identity = 129/400 (32.25%), Postives = 214/400 (53.50%), Query Frame = 0

Query: 43  SGYLPINPASSSAIFFAFYEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFFELGPWRVNF 102
           SGY  +N  +++ +F+ FYE+Q   SP S  PL++WL GGPGCSS+   F+E GP+ VN 
Sbjct: 27  SGYFNVNETTNANLFYLFYESQN--SP-STDPLILWLTGGPGCSSLMAAFYENGPYFVN- 86

Query: 103 HKEKTDPISLIPNPGSWNRRFGVLFLDNPIGTGFSIAATEDEIPRDQYSVSKHLFPAISS 162
                D ++L  NP SWN    VL++D+P+G GFS     D     +  +S++L+  ++ 
Sbjct: 87  -----DNLTLSENPNSWNMVANVLYVDSPLGAGFSYVVDSDGYSTTETEISENLYSFLTQ 146

Query: 163 FIELDLAFKSRPIYITGESYAGKYVPSIGYYILKKNPKLPVGKRLNLAGVAIGDGLTDPI 222
           F+     +   P+YI GESYAG YVPS  YYI +KN  L     +NL G+AIG+G+ DP 
Sbjct: 147 FLSKYPKYSKLPLYIFGESYAGHYVPSFSYYIYQKNLGLAT---INLKGLAIGNGMVDPY 206

Query: 223 TQVATHGLNAFYSGLINEKQRKELEVAQMEAVELTKMGNWSEATNARIRVLELLKNMTGL 282
            Q  + G  A+  G+++    KE E       +    G+++  T     ++++++   G 
Sbjct: 207 IQYGSLGPFAYAHGMLDINALKETEGLYESCQQAIDSGDYNMTTQICNNIMDIVQEYAGN 266

Query: 283 ATLYDYTRKA------PYNSDLVAEFLSSKEVKRALGVNESMVYEECSDLVGEILSDDVM 342
             +YD ++         YN   + ++L+    K++ GV  +  +  CS      +  D  
Sbjct: 267 FNVYDVSKTCYPNEPLCYNFTAIIDYLNLASTKQSFGVLPNSTWNVCSTQPYSAIIRDWF 326

Query: 343 KSVKYKVDFLVKNSKVLLYQGQYDLRDGVVSTEAWVKKLKWEEIERFLEADRKIWRV-GD 402
            +    +  L++N KVL+Y G YD     + +  W  +LKW+  + F  + RKI  + G+
Sbjct: 327 NTPINYIPTLLENYKVLVYNGNYDWICNFLGSTEWTSQLKWKYNQEFNNSPRKILYINGN 386

Query: 403 ELAGYVQKWKSLTNVVVLGAGHLLPTDQALNSQAMIEDWV 436
            ++GY Q + +LT  V+LGA H+ P +  + + AM+E ++
Sbjct: 387 TISGYSQSYDNLTMQVLLGASHMAPREAPVAALAMVESFI 414

BLAST of Spg028734 vs. ExPASy Swiss-Prot
Match: C9WMM5 (Venom serine carboxypeptidase OS=Apis mellifera OX=7460 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 2.5e-59
Identity = 143/412 (34.71%), Postives = 225/412 (54.61%), Query Frame = 0

Query: 38  ALPTKSGYLPINPASSSAIFFAFYEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFFELGP 97
           A+ + +G+L +N   +S +FF F+ A   +      P+++WLQGGPG +SM G F E GP
Sbjct: 68  AISSYAGFLTVNKKYNSNMFFWFFPA---LHDPKTAPVVLWLQGGPGATSMYGLFLENGP 127

Query: 98  WRVNFHKEKTDPISLIPNPGSWNRRFGVLFLDNPIGTGFSIAATEDEIPRDQYSVSKHLF 157
           + V  +K      +L     SWN+   +L++DNP+GTGFS    E     ++  V + + 
Sbjct: 128 FIVTKNK------TLKMREYSWNKCHNLLYIDNPVGTGFSFTEDERGYATNETHVGRDVH 187

Query: 158 PAISSFIELDLAFKSRPIYITGESYAGKYVPSIGYYILKKNPKLPVGKRLNLAGVAIGDG 217
            A+  F EL    ++   Y+TGESY GKYVP++ + I   N K  +  ++NL G+AIG+G
Sbjct: 188 TALVQFFELFPELQTNDFYVTGESYGGKYVPAVSHAIKDYNIKAKI--KINLKGLAIGNG 247

Query: 218 LTDPITQVATHGLNAFYSGLINEKQRKELEVAQMEAVELTKMGNWSEATNARIRVLE--- 277
           LTDP+ Q+  +G   +  GL++   R   +  + +   L K   W EA +    +L+   
Sbjct: 248 LTDPVNQL-DYGDYLYQLGLLDANGRNLFQKYEEQGKNLIKQEKWLEAFDLFDELLDGDI 307

Query: 278 -----LLKNMTGLATLYDYT-RKAPYN-SDLVAEFLSSKEVKRALGVNESMVYEECSDLV 337
                L KN+TG    ++Y   K P N SD + E+L   +V++A+ V       E S  V
Sbjct: 308 TQQPSLYKNLTGFDYYFNYLHEKDPSNDSDYMVEWLQRADVRKAIHVGNRTFIPE-SKKV 367

Query: 338 GEILSDDVMKSVKYKVDFLVKNSKVLLYQGQYDLRDGVVSTEAWVKKLKWEEIERFLEAD 397
            + +  DVM+S+   +  L ++ +VL+Y GQ D+      TE +++KLKW   E++  A 
Sbjct: 368 EKYMKADVMQSLAVLIADLTQHYRVLIYNGQLDIIVAYPLTENYLQKLKWPGAEKYKTAQ 427

Query: 398 RKIWRVGDELAGYVQKWKSLTNVVVLGAGHLLPTDQALNSQAMIEDWVLEKG 440
           RK+W VG+ELAGY +   SLT V+V  AGH++P DQ   +  +I  +   KG
Sbjct: 428 RKVWFVGNELAGYSKTVDSLTEVLVRNAGHMVPLDQPKWALDLITRFTHNKG 466

BLAST of Spg028734 vs. ExPASy TrEMBL
Match: A0A6J1IKV2 (Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111477961 PE=3 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 8.6e-228
Identity = 387/460 (84.13%), Postives = 427/460 (92.83%), Query Frame = 0

Query: 1   MDSSNFLLSIFFSFFSFFFFASFPFPATSSPPFFPAGALPTKSGYLPINPASSSAIFFAF 60
           MDS+N LLS+FF    F FFASFP PATSSPPFFPA ALPTKSGYLPINP + SAI+FAF
Sbjct: 1   MDSTNVLLSVFF----FLFFASFPVPATSSPPFFPAEALPTKSGYLPINPTTGSAIYFAF 60

Query: 61  YEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFFELGPWRVNFHKEKTDPISLIPNPGSWN 120
           YEAQ P+S +SKTPLLIWLQGGPGCSSM GNFFELGPWRVNFHK++TDP+SLIPN GSWN
Sbjct: 61  YEAQTPISSISKTPLLIWLQGGPGCSSMIGNFFELGPWRVNFHKKETDPVSLIPNTGSWN 120

Query: 121 RRFGVLFLDNPIGTGFSIAATEDEIPRDQYSVSKHLFPAISSFIELDLAFKSRPIYITGE 180
           RRFGVLFLDNPIGTGFSIAAT+DEIP++QYSVSK+LF AISSFIE D+ FK+RPIYITGE
Sbjct: 121 RRFGVLFLDNPIGTGFSIAATKDEIPKNQYSVSKYLFSAISSFIEQDITFKNRPIYITGE 180

Query: 181 SYAGKYVPSIGYYILKKNPKLPVGKRLNLAGVAIGDGLTDPITQVATHGLNAFYSGLINE 240
           SYAGKYVPSIGYYILKKNPKLPV K+LNLAGVAIG+GLTDPITQVATH LNA+YSGLINE
Sbjct: 181 SYAGKYVPSIGYYILKKNPKLPVDKKLNLAGVAIGNGLTDPITQVATHALNAYYSGLINE 240

Query: 241 KQRKELEVAQMEAVELTKMGNWSEATNARIRVLELLKNMTGLATLYDYTRKAPYNSDLVA 300
           +Q+KE+E+AQ+EAVELTK GNWSEATNAR +VL LLKNMTGLATLYDYTRK+PYNS+LVA
Sbjct: 241 RQKKEMEIAQLEAVELTKKGNWSEATNARSKVLNLLKNMTGLATLYDYTRKSPYNSELVA 300

Query: 301 EFLSSKEVKRALGVNESMVYEECSDLVGEILSDDVMKSVKYKVDFLVKNSKVLLYQGQYD 360
           EFLS KEVKRALGVNESMV+EECSDLVGE+L DDVMKS++YKVD+LVKNSKVL+YQGQ+D
Sbjct: 301 EFLSFKEVKRALGVNESMVFEECSDLVGEVLKDDVMKSMRYKVDYLVKNSKVLVYQGQFD 360

Query: 361 LRDGVVSTEAWVKKLKWEEIERFLEADRKIWRVGDELAGYVQKWKSLTNVVVLGAGHLLP 420
           LRDGVVSTEAW+KK+KW EIERFL ADRKIW VGDELAGYVQKWKSL+N VVLGAGHL+P
Sbjct: 361 LRDGVVSTEAWLKKVKWGEIERFLGADRKIWSVGDELAGYVQKWKSLSNAVVLGAGHLVP 420

Query: 421 TDQALNSQAMIEDWVLEKGVFGDDKAQKKKPFATTGFGVW 461
           +DQALNSQAMIEDWVLEKGVFGDD A++KKPF    FGVW
Sbjct: 421 SDQALNSQAMIEDWVLEKGVFGDDNAEEKKPFGANDFGVW 456

BLAST of Spg028734 vs. ExPASy TrEMBL
Match: A0A6J1FP80 (Carboxypeptidase OS=Cucurbita moschata OX=3662 GN=LOC111446989 PE=3 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 4.7e-226
Identity = 385/460 (83.70%), Postives = 426/460 (92.61%), Query Frame = 0

Query: 1   MDSSNFLLSIFFSFFSFFFFASFPFPATSSPPFFPAGALPTKSGYLPINPASSSAIFFAF 60
           MDS+N LLS+FF    F FFASFP  ATSSPPFFPA ALPTKSGYLPINP++ SAI+FAF
Sbjct: 1   MDSTNVLLSVFF----FLFFASFPVLATSSPPFFPAEALPTKSGYLPINPSTGSAIYFAF 60

Query: 61  YEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFFELGPWRVNFHKEKTDPISLIPNPGSWN 120
           YEAQ P+S +SKTPLLIWLQGGPGCSSM GNFFELGPWRVNFHK++TDP+SLIPN GSWN
Sbjct: 61  YEAQTPISSISKTPLLIWLQGGPGCSSMIGNFFELGPWRVNFHKKETDPVSLIPNTGSWN 120

Query: 121 RRFGVLFLDNPIGTGFSIAATEDEIPRDQYSVSKHLFPAISSFIELDLAFKSRPIYITGE 180
           RRFGVLFLDNPIGTGFSIAAT+DEIP++QYSVSKHLF AISSFIE D+ FK+RPIYITGE
Sbjct: 121 RRFGVLFLDNPIGTGFSIAATKDEIPKNQYSVSKHLFSAISSFIEQDITFKNRPIYITGE 180

Query: 181 SYAGKYVPSIGYYILKKNPKLPVGKRLNLAGVAIGDGLTDPITQVATHGLNAFYSGLINE 240
           SYAGKYVPSIGYYILKKN KLPV KRLNLAGVAIG+GLTDPITQVATH LNA+YSGLINE
Sbjct: 181 SYAGKYVPSIGYYILKKNHKLPVDKRLNLAGVAIGNGLTDPITQVATHALNAYYSGLINE 240

Query: 241 KQRKELEVAQMEAVELTKMGNWSEATNARIRVLELLKNMTGLATLYDYTRKAPYNSDLVA 300
           +Q+KE+E+AQ+EAVELT+ GNWSEATNAR +VL LLKNMTGLATLYDYTRK+PYNS+LVA
Sbjct: 241 RQKKEMEIAQLEAVELTRKGNWSEATNARSKVLNLLKNMTGLATLYDYTRKSPYNSELVA 300

Query: 301 EFLSSKEVKRALGVNESMVYEECSDLVGEILSDDVMKSVKYKVDFLVKNSKVLLYQGQYD 360
           EFLS KEVKRALGVNESMV+EECSDLVGE+L DDVMKS++YKVD+LVKNSKVL+YQGQ+D
Sbjct: 301 EFLSFKEVKRALGVNESMVFEECSDLVGEVLKDDVMKSMRYKVDYLVKNSKVLVYQGQFD 360

Query: 361 LRDGVVSTEAWVKKLKWEEIERFLEADRKIWRVGDELAGYVQKWKSLTNVVVLGAGHLLP 420
           LRDGVVSTEAW+KK+KW EIERFL ADRKIWRVGDELAGYVQ WKSL+N VVLGAGHL+P
Sbjct: 361 LRDGVVSTEAWLKKVKWGEIERFLGADRKIWRVGDELAGYVQTWKSLSNAVVLGAGHLVP 420

Query: 421 TDQALNSQAMIEDWVLEKGVFGDDKAQKKKPFATTGFGVW 461
           +DQALNSQAMIEDWVLEKGVFGDD A++K+PF    FGVW
Sbjct: 421 SDQALNSQAMIEDWVLEKGVFGDDNAEEKRPFGANDFGVW 456

BLAST of Spg028734 vs. ExPASy TrEMBL
Match: A0A5A7UN23 (Carboxypeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G001750 PE=3 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 3.5e-221
Identity = 380/463 (82.07%), Postives = 419/463 (90.50%), Query Frame = 0

Query: 1   MDSSNFLLSIFFSFFSFFFFASFPFPATSSPPFFPAGALPTKSGYLPINPASSSAIFFAF 60
           MDS+NFLLS+F     FFFF SFP P+TSS PFFP  ALPTKSGYLP+NP + SAI+FAF
Sbjct: 1   MDSANFLLSVF-----FFFFTSFPSPSTSSRPFFPPEALPTKSGYLPVNPTTGSAIYFAF 60

Query: 61  YEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFFELGPWRVNFHKEKTDPISLIPNPGSWN 120
           YEAQ P+SP+SKTPLLIWLQGGPGCSSM GNFFELGPWRVNF+K+KT+PISLIPNPGSWN
Sbjct: 61  YEAQTPISPISKTPLLIWLQGGPGCSSMVGNFFELGPWRVNFNKQKTEPISLIPNPGSWN 120

Query: 121 RRFGVLFLDNPIGTGFSIAATEDEIPRDQYSVSKHLFPAISSFIELDLAFKSRPIYITGE 180
           R FG+LFLDNPIGTGFSIA T+DEIP++QYSVS+HLF AISSFIEL+ AFK+RPIYITGE
Sbjct: 121 RIFGLLFLDNPIGTGFSIATTKDEIPKNQYSVSRHLFTAISSFIELNSAFKNRPIYITGE 180

Query: 181 SYAGKYVPSIGYYILKKNPKLPVGKRLNLAGVAIGDGLTDPITQVATHGLNAFYSGLINE 240
           SYAGKYVPSIGYYILKKNP+LP  KR+NL GVAIGDGLTDPITQVATHGLNAFYSGLINE
Sbjct: 181 SYAGKYVPSIGYYILKKNPRLPYDKRVNLVGVAIGDGLTDPITQVATHGLNAFYSGLINE 240

Query: 241 KQRKELEVAQMEAVELTKMGNWSEATNARIRVLELLKNMTGLATLYDYTRKAPYNSDLVA 300
           KQ++E+EVAQ+E VEL K+G WSEATNAR +VL+LLKNMTGLATLYDYTRK+PYN+DLV 
Sbjct: 241 KQKEEMEVAQVETVELIKLGKWSEATNARFKVLDLLKNMTGLATLYDYTRKSPYNTDLVD 300

Query: 301 EFLSSKEVKRALGVNESMVYEECSDLVGEILSDDVMKSVKYKVDFLVKNSKVLLYQGQYD 360
           EFLS K+VKR LGVNESMV+E CSD+VGE+L+DDVMKSV+YKVDFLVKNSKVLLYQGQYD
Sbjct: 301 EFLSFKKVKRVLGVNESMVFEGCSDVVGEVLNDDVMKSVRYKVDFLVKNSKVLLYQGQYD 360

Query: 361 LRDGVVSTEAWVKKLKWEEIERFLEADRKIWRVGDELAGYVQKWKSLTNVVVLGAGHLLP 420
           LRDGVVSTEAW+KKLKWEEIE +L  DRKIWRVGDELAGYVQKWKSLTNVVVLGAGHLLP
Sbjct: 361 LRDGVVSTEAWLKKLKWEEIENYLGVDRKIWRVGDELAGYVQKWKSLTNVVVLGAGHLLP 420

Query: 421 TDQALNSQAMIEDWVLEKGVFG---DDKAQKKKPFATTGFGVW 461
           TDQ LNSQAMIEDWVLEKGVF    DD AQ KK  +   FG+W
Sbjct: 421 TDQTLNSQAMIEDWVLEKGVFSDGDDDDAQVKKASSFNDFGLW 458

BLAST of Spg028734 vs. ExPASy TrEMBL
Match: A0A1S3C0A8 (Carboxypeptidase OS=Cucumis melo OX=3656 GN=LOC103495166 PE=3 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 6.2e-218
Identity = 371/447 (83.00%), Postives = 408/447 (91.28%), Query Frame = 0

Query: 17  FFFFASFPFPATSSPPFFPAGALPTKSGYLPINPASSSAIFFAFYEAQAPVSPLSKTPLL 76
           FFFF SFP P+TSS PFFP  ALPTKSGYLP+NP + SAI+FAFYEAQ P+SP+SKTPLL
Sbjct: 28  FFFFTSFPSPSTSSRPFFPPEALPTKSGYLPVNPTTGSAIYFAFYEAQTPISPISKTPLL 87

Query: 77  IWLQGGPGCSSMTGNFFELGPWRVNFHKEKTDPISLIPNPGSWNRRFGVLFLDNPIGTGF 136
           IWLQGGPGCSSM GNFFELGPWRVNF+K+KT+PISLIPNPGSWNR FG+LFLDNPIGTGF
Sbjct: 88  IWLQGGPGCSSMVGNFFELGPWRVNFNKQKTEPISLIPNPGSWNRIFGLLFLDNPIGTGF 147

Query: 137 SIAATEDEIPRDQYSVSKHLFPAISSFIELDLAFKSRPIYITGESYAGKYVPSIGYYILK 196
           SIA T+DEIP++QYSVS+HLF AISSFIEL+ AFK+RPIYITGESYAGKYVPSIGYYILK
Sbjct: 148 SIATTKDEIPKNQYSVSRHLFTAISSFIELNSAFKNRPIYITGESYAGKYVPSIGYYILK 207

Query: 197 KNPKLPVGKRLNLAGVAIGDGLTDPITQVATHGLNAFYSGLINEKQRKELEVAQMEAVEL 256
           KNP+LP  KR+NL GVAIGDGLTDPITQVATHGLNAFYSGLINEKQ++E+EVAQ+E VEL
Sbjct: 208 KNPRLPYDKRVNLVGVAIGDGLTDPITQVATHGLNAFYSGLINEKQKEEMEVAQVETVEL 267

Query: 257 TKMGNWSEATNARIRVLELLKNMTGLATLYDYTRKAPYNSDLVAEFLSSKEVKRALGVNE 316
            K+G WSEATNAR +VL+LLKNMTGLATLYDYTRK+PYN+DLV EFLS K+VKR LGVNE
Sbjct: 268 IKLGKWSEATNARFKVLDLLKNMTGLATLYDYTRKSPYNTDLVDEFLSFKKVKRVLGVNE 327

Query: 317 SMVYEECSDLVGEILSDDVMKSVKYKVDFLVKNSKVLLYQGQYDLRDGVVSTEAWVKKLK 376
           SMV+E CSD+VGE+L+DDVMKSV+YKVDFLVKNSKVLLYQGQYDLRDGVVSTEAW+KKLK
Sbjct: 328 SMVFEGCSDVVGEVLNDDVMKSVRYKVDFLVKNSKVLLYQGQYDLRDGVVSTEAWLKKLK 387

Query: 377 WEEIERFLEADRKIWRVGDELAGYVQKWKSLTNVVVLGAGHLLPTDQALNSQAMIEDWVL 436
           WEEIE +L  DRKIWRVGDELAGYVQKWKSLTNVVVLGAGHLLPTDQ LNSQAMIEDWVL
Sbjct: 388 WEEIENYLGVDRKIWRVGDELAGYVQKWKSLTNVVVLGAGHLLPTDQTLNSQAMIEDWVL 447

Query: 437 EKGVFG---DDKAQKKKPFATTGFGVW 461
           EKGVF    DD AQ KK  +   FG+W
Sbjct: 448 EKGVFSDGDDDDAQVKKASSFNDFGLW 474

BLAST of Spg028734 vs. ExPASy TrEMBL
Match: A0A6J1D7G2 (Carboxypeptidase OS=Momordica charantia OX=3673 GN=LOC111018332 PE=3 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 4.7e-210
Identity = 360/447 (80.54%), Postives = 405/447 (90.60%), Query Frame = 0

Query: 16  SFFFFASFPF-PATSSPPFFPAGALPTKSGYLPINPASSSAIFFAFYEAQAPVSPLSKTP 75
           +F F   F F  A +S PFFPAGALPTKSGYLP++PAS SA++FAFYEAQAP+SPL KTP
Sbjct: 9   NFIFLLCFLFSAAAASSPFFPAGALPTKSGYLPVSPASESAVYFAFYEAQAPISPLPKTP 68

Query: 76  LLIWLQGGPGCSSMTGNFFELGPWRVNFHKEKTDPISLIPNPGSWNRRFGVLFLDNPIGT 135
           LLIWLQGGPGCSSM GNFFELGPWR+NFHK+++DP+ L PNP SWNRRFG+LFLDNPIGT
Sbjct: 69  LLIWLQGGPGCSSMIGNFFELGPWRLNFHKKESDPLLLQPNPASWNRRFGLLFLDNPIGT 128

Query: 136 GFSIAATEDEIPRDQYSVSKHLFPAISSFIELDLAFKSRPIYITGESYAGKYVPSIGYYI 195
           GFSIAA  DEIPRDQYSVSKHLF AIS+F+ELD  F++RPIYITGESYAGKYVPSIGYYI
Sbjct: 129 GFSIAAAADEIPRDQYSVSKHLFAAISAFLELDSGFRNRPIYITGESYAGKYVPSIGYYI 188

Query: 196 LKKNPKLPVGKRLNLAGVAIGDGLTDPITQVATHGLNAFYSGLINEKQRKELEVAQMEAV 255
           LKKNPKL   KR+ LAGVAIGDGLTDP TQVATH LNAFYSG +NE+Q+KELE+AQ EAV
Sbjct: 189 LKKNPKLAAAKRVALAGVAIGDGLTDPATQVATHALNAFYSGFVNERQKKELEIAQTEAV 248

Query: 256 ELTKMGNWSEATNARIRVLELLKNMTGLATLYDYTRKAPYNSDLVAEFLSSKEVKRALGV 315
           ELTKMGNWSEAT+ARIRVL+LL+NMTGLATLYDYTRKAPYN +LVAEFL+SK VK+A+GV
Sbjct: 249 ELTKMGNWSEATSARIRVLDLLQNMTGLATLYDYTRKAPYNDELVAEFLNSKGVKKAMGV 308

Query: 316 NESMVYEECSDLVGEILSDDVMKSVKYKVDFLVKNSKVLLYQGQYDLRDGVVSTEAWVKK 375
           NES+V+EECSD+VGEIL +DVMKSV+YKVDFLVK +KVLLYQGQ+DLRDGVVSTEAWVKK
Sbjct: 309 NESLVFEECSDVVGEILHEDVMKSVRYKVDFLVKKTKVLLYQGQFDLRDGVVSTEAWVKK 368

Query: 376 LKWEEIERFLEADRKIWRVGD-ELAGYVQKWKSLTNVVVLGAGHLLPTDQALNSQAMIED 435
           LKWEEIE FLEA+RKIWRV + E+AGYVQKWKSL+NVVVLGAGHLLPTD+ALNSQAMIED
Sbjct: 369 LKWEEIESFLEAERKIWRVNNGEVAGYVQKWKSLSNVVVLGAGHLLPTDKALNSQAMIED 428

Query: 436 WVLEKGVFGDDKAQKKKPFATTGFGVW 461
           WVLEKG F DDK ++K+ F  + FGVW
Sbjct: 429 WVLEKGAFSDDKGEEKRHFG-SDFGVW 454

BLAST of Spg028734 vs. TAIR 10
Match: AT1G15000.1 (serine carboxypeptidase-like 50 )

HSP 1 Score: 495.4 bits (1274), Expect = 5.1e-140
Identity = 239/413 (57.87%), Postives = 311/413 (75.30%), Query Frame = 0

Query: 31  PPFFPAGALPTKSGYLPINPASSSAIFFAFYEAQAPVSPLSKTPLLIWLQGGPGCSSMTG 90
           PP FP  ALPTKSGYLP+ PA  S++F+AFYEAQ P +PL  TPLL+WLQGGPGCSSM G
Sbjct: 25  PPLFPDEALPTKSGYLPVKPAPGSSMFYAFYEAQEPTTPLPDTPLLVWLQGGPGCSSMIG 84

Query: 91  NFFELGPWRVNFHKEKTDPISLIPNPGSWNRRFGVLFLDNPIGTGFSIAATEDEIPRDQY 150
           NF+ELGPWRV      TD   L  NPG+WNR FG+LF+DNPIG GFSIAA++ +IP +Q 
Sbjct: 85  NFYELGPWRV--VSRATD---LERNPGAWNRLFGLLFVDNPIGVGFSIAASQQDIPTNQR 144

Query: 151 SVSKHLFPAISSFIELDLAFKSRPIYITGESYAGKYVPSIGYYILKKNPKLPVGKRLNLA 210
            V++HL+ A+  F+E + +F++RP+Y TGESYAGKYVP+IGYYILK+ P      ++NL 
Sbjct: 145 QVAEHLYAALVEFLEQNPSFENRPVYFTGESYAGKYVPAIGYYILKEKP----NGKVNLK 204

Query: 211 GVAIGDGLTDPITQVATHGLNAFYSGLINEKQRKELEVAQMEAVELTKMGNWSEATNARI 270
           G+AIG+GLTDP+TQV TH +N +YSGL+N KQR EL+ AQ  +V L K   W EA +AR 
Sbjct: 205 GLAIGNGLTDPVTQVQTHAVNVYYSGLVNAKQRVELQKAQEISVALVKSQKWREAADART 264

Query: 271 RVLELLKNMTGLATLYDYTRKAPYNSDLVAEFLSSKEVKRALGVNESMVYEECSDLVGEI 330
            +L LL NMTGLATLY+  R  PY +DLV + L+ +E KR LGV+E++ +EECSD V ++
Sbjct: 265 ELLTLLSNMTGLATLYNTARAIPYRTDLVVDLLNQREAKRVLGVSETVRFEECSDEVEDV 324

Query: 331 LSDDVMKSVKYKVDFLVKNSKVLLYQGQYDLRDGVVSTEAWVKKLKWEEIERFLEADRKI 390
           L  DVMKSVK+ V++ ++ ++VLLYQG  DLRDGVVSTE W+K + W  +  F  A+R++
Sbjct: 325 LRADVMKSVKFMVEYALERTQVLLYQGMLDLRDGVVSTEEWMKTMNWSGLGMFSTAERRV 384

Query: 391 WRVGD-ELAGYVQKWKSLTNVVVLGAGHLLPTDQALNSQAMIEDWVLEKGVFG 443
           W+  D  +AGYVQ+W +L +V V GAGH +PTD+A+NS+ MIE WVL KG+FG
Sbjct: 385 WKDEDGVVAGYVQRWGNLCHVAVTGAGHFVPTDKAVNSRDMIEGWVLGKGLFG 428

BLAST of Spg028734 vs. TAIR 10
Match: AT5G22980.1 (serine carboxypeptidase-like 47 )

HSP 1 Score: 195.7 bits (496), Expect = 8.4e-50
Identity = 122/408 (29.90%), Postives = 213/408 (52.21%), Query Frame = 0

Query: 43  SGYLPINPASSSAIFFAFYEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFFELGPWRVNF 102
           +GY  +  + S+ +F+ F+E++   +     P++IWL GGPGCSS    F+E GP+++  
Sbjct: 100 AGYYSLPHSKSAKMFYFFFESRNKTT----DPVVIWLTGGPGCSSSVAMFYENGPFKI-- 159

Query: 103 HKEKTDPISLIPNPGSWNRRFGVLFLDNPIGTGFSIAATEDEIPRDQYSVSKHLFPAISS 162
               +  +SL  N   W++   ++++D P+GTGFS  + E +I  D+  VS  L+  + +
Sbjct: 160 ----SKDLSLYWNDFGWDKVSNIIYVDQPVGTGFSYTSDESDIRNDEDGVSNDLYDFLQA 219

Query: 163 FIELDLAFKSRPIYITGESYAGKYVPSIGYYILKKNPKLPVGKRLNLAGVAIGDGLTDPI 222
           F +    F     +ITGESYAG Y+P++   +   N K   G  +NL G AIG+GLT+P 
Sbjct: 220 FFKEHPKFVKNDFFITGESYAGHYIPALASRVHSGNKK-KEGIPINLKGFAIGNGLTNPE 279

Query: 223 TQVATHGLNAFYSGLINEKQRKELEVAQMEAVELTKMGNWSE--ATNARIRVL-----EL 282
            Q   +G  A    LI+E   + L+   +E   +TK  +       ++ + V      ++
Sbjct: 280 IQYGAYGDYALQMKLISESDHESLKQDYVECQNITKKCSLGGGLVCDSAVEVCTSIFNKI 339

Query: 283 LKNMTGLATLYDYTRKAP----YNSDLVAEFLSSKEVKRALGVNESMVYEECSDLVGEIL 342
           +   +GL   YD  +K      Y+   +  FL+ + V++ALGV + + +  CS  V + +
Sbjct: 340 VAKKSGL-NYYDIRKKCVGSLCYDFSRMEIFLNKENVRKALGVGD-IKFVSCSSTVYDAM 399

Query: 343 SDDVMKSVKYKVDFLVKNS-KVLLYQGQYDLRDGVVSTEAWVKKLKWEEIERFLEADRKI 402
            +D M++++ K+  LV +   +L+Y G+YDL    +    WV ++ W   + F  A    
Sbjct: 400 IEDWMQNLEVKIPSLVNDGINLLVYAGEYDLICNWLGNSRWVDQMNWSGQKGFGSAKNVS 459

Query: 403 WRVGDELAGYVQKWKSLTNVVVLGAGHLLPTDQALNSQAMIEDWVLEK 439
           + V  + AG ++    LT + V  AGH++P DQ   S  M+++W+  K
Sbjct: 460 FLVDGKEAGLLKNHGPLTFLKVYNAGHMVPMDQPKASLQMLQNWMQGK 494

BLAST of Spg028734 vs. TAIR 10
Match: AT3G45010.1 (serine carboxypeptidase-like 48 )

HSP 1 Score: 192.6 bits (488), Expect = 7.1e-49
Identity = 118/409 (28.85%), Postives = 210/409 (51.34%), Query Frame = 0

Query: 43  SGYLPINPASSSAIFFAFYEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFFELGPWRVNF 102
           +GY  +  + ++ +F+ F+E++         P++IWL GGPGCSS    F+E GP+ V+ 
Sbjct: 103 AGYYKLPNSKAARMFYFFFESRTN----KADPVVIWLTGGPGCSSELALFYENGPFTVSN 162

Query: 103 HKEKTDPISLIPNPGSWNRRFGVLFLDNPIGTGFSIAATEDEIPRDQYSVSKHLFPAISS 162
           +       SL  N   W++   ++++D P+GTGFS  + + ++  D+  VS  L+  + +
Sbjct: 163 NS------SLSWNEFGWDKASNLIYVDQPVGTGFSYTSDQSDLRHDEDGVSNDLYDFLQA 222

Query: 163 FIELDLAFKSRPIYITGESYAGKYVPSIGYYILKKNPKLPVGKRLNLAGVAIGDGLTDPI 222
           F +    F     YITGESYAG Y+P++   + + N K   G  +NL G AIG+GLT+P 
Sbjct: 223 FFKEHPQFVKNDFYITGESYAGHYIPALASRVHRGN-KNKEGTHINLKGFAIGNGLTNPE 282

Query: 223 TQVATHGLNAFYSGLINEKQRKELE----VAQMEAVELTKMGNWSEATNARI----RVLE 282
            Q   +   A    LI +     L       Q    E +  G   +A  +       + +
Sbjct: 283 IQYGAYADYALDMNLITQSDHDNLNRYYATCQQSIKECSADGGEGDACASSYTVCNNIFQ 342

Query: 283 LLKNMTGLATLYDYTRKAP----YNSDLVAEFLSSKEVKRALGVNESMVYEECSDLVGEI 342
            + ++ G    YD  ++      Y+   +  FL+ K V++ALGV + + +  CS  V E 
Sbjct: 343 KIMDIAGNVNYYDVRKQCEGSLCYDFSNMENFLNQKSVRKALGVGD-IEFVSCSTAVYEA 402

Query: 343 LSDDVMKSVKYKVDFLVKNS-KVLLYQGQYDLRDGVVSTEAWVKKLKWEEIERFLEADRK 402
           +  D M++++  +  L+++  K+L+Y G+YDL    +    WV +++W   + F+ A   
Sbjct: 403 MQMDWMRNLEVGIPALLQDGIKLLVYAGEYDLICNWLGNSKWVHEMEWSGQKEFVAAATV 462

Query: 403 IWRVGDELAGYVQKWKSLTNVVVLGAGHLLPTDQALNSQAMIEDWVLEK 439
            + V ++ AG ++ + SLT + V  AGH++P DQ   +  M+++W+  K
Sbjct: 463 PFHVDNKEAGLMKNYGSLTFLKVHDAGHMVPMDQPKAALQMLQNWMQGK 499

BLAST of Spg028734 vs. TAIR 10
Match: AT3G10410.1 (SERINE CARBOXYPEPTIDASE-LIKE 49 )

HSP 1 Score: 181.4 bits (459), Expect = 1.6e-45
Identity = 117/404 (28.96%), Postives = 200/404 (49.50%), Query Frame = 0

Query: 43  SGYLPINPASSSAIFFAFYEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFFELGPWRVNF 102
           +GY  +  +  +++F+ F+E++         P++IWL GGPGCSS    F+E GP+++  
Sbjct: 101 AGYYKLPKSRGASMFYFFFESRNK----KDAPVVIWLTGGPGCSSELAVFYENGPFKI-- 160

Query: 103 HKEKTDPISLIPNPGSWNRRFGVLFLDNPIGTGFSIAATEDEIPRDQYSVSKHLFPAISS 162
               T  +SL  N   W++   +L++D P+GTGFS    + +I  D+  VS  L+  + +
Sbjct: 161 ----TSNMSLAWNEYGWDQVSNLLYVDQPVGTGFSYTTDKSDIRHDETGVSNDLYDFLQA 220

Query: 163 FIELDLAFKSRPIYITGESYAGKYVPSIGYYILKKNPKLPVGKRLNLAGVAIGDGLTDPI 222
           F            YITGESYAG Y+P+    + K N K   G  +NL G AIG+GLTDP 
Sbjct: 221 FFAEHPKLAKNDFYITGESYAGHYIPAFASRVHKGN-KANEGVHINLKGFAIGNGLTDPA 280

Query: 223 TQVATHGLNAFYSGLINEKQRKELEVAQMEAVELTKMGNWSEATNARIRVLELLKNMTGL 282
            Q   +   A   GLI +K+   LE          K+      T+     L      +G+
Sbjct: 281 LQYPAYPDYALEMGLITQKEHDRLEKIVPLCELSIKLCGTDGTTSCLASYLVCNSLFSGV 340

Query: 283 ------ATLYDYTRKAP----YNSDLVAEFLSSKEVKRALGVNESMVYEECSDLVGEILS 342
                    YD  +K      Y+   + +FL+ + V+++LGV + + +  CS  V + + 
Sbjct: 341 MSHAGGVNYYDIRKKCVGSLCYDFSNMEKFLNLQSVRKSLGVGD-IDFVSCSTSVYQAML 400

Query: 343 DDVMKSVKYKVDFLVKNS-KVLLYQGQYDLRDGVVSTEAWVKKLKWEEIERFLEADRKIW 402
            D M++++  +  L+++   +L+Y G+YDL    +    WV  ++W     F  A    +
Sbjct: 401 VDWMRNLEVGIPTLLEDGISLLVYAGEYDLICNWLGNSRWVNAMEWSGKTNFGAAKEVPF 460

Query: 403 RVGDELAGYVQKWKSLTNVVVLGAGHLLPTDQALNSQAMIEDWV 436
            V  + AG ++ ++ L+ + V  AGH++P DQ   +  M++ W+
Sbjct: 461 IVDGKEAGLLKTYEQLSFLKVRDAGHMVPMDQPKAALKMLKRWM 492

BLAST of Spg028734 vs. TAIR 10
Match: AT3G52010.1 (serine carboxypeptidase-like 37 )

HSP 1 Score: 160.2 bits (404), Expect = 3.9e-39
Identity = 120/411 (29.20%), Postives = 207/411 (50.36%), Query Frame = 0

Query: 44  GYLPINPASSSAIFFAFYEAQAPVSPLSKTPLLIWLQGGPGCSSMTGNFFELGPWRVNFH 103
           GY+P+N  SS  +++ F EA   + P + TPL+IW  GGP CSS+ G F ELGP+RV+  
Sbjct: 82  GYVPVNEPSSRFLYYYFVEA---IKPNTSTPLVIWFNGGPACSSLGGAFLELGPFRVHSG 141

Query: 104 KEKTDPISLIPNPGSWNRRFGVLFLDNPIGTGFSIAAT----EDEIPRDQYSVSKHLFPA 163
             K     L  NP SWN    VLFL++P+ TGFS ++     E+   +   + ++  +  
Sbjct: 142 GRK-----LFRNPYSWNNEANVLFLESPVTTGFSYSSNPIDLEELGEKGDKATAEDNYIF 201

Query: 164 ISSFIELDLAFKSRPIYITGESYAGKYVPSIGYYILKKNPKLPVGKRLNLAGVAIGD--- 223
           + +++E    +K R IYI G+SYAG YVP +   I+ +N K  V    NL G+ IG+   
Sbjct: 202 LMNWLERFPEYKGRDIYIAGQSYAGHYVPQLAQIIIHRNKKTLV----NLRGILIGNPSL 261

Query: 224 --GLTDPITQ--VATHGLNAFYSGLINEKQ---RKELEVAQMEAVELTKMGNWSEATNAR 283
              + DP     + +HGL +    + N  Q   R +L      A+ +  +    +     
Sbjct: 262 LTSIQDPYGYEFMLSHGLMS-QQQMDNYNQFCLRDDLYDNDKCALSVKTI----DDAKKH 321

Query: 284 IRVLELLKNMTGLATLYDYTRKA-------PYNSDLVAEFLSSKEVKRALGVNESMV--- 343
           +    +   +   +TL   ++K        P + D +  +L+ K+V++A+  N + +   
Sbjct: 322 LDTYNIYAPVCLNSTLSRISKKCTTVLEVDPCSKDYLKAYLNRKKVQKAIHANTTKLPYE 381

Query: 344 YEECSDLVGEILSDDVMKSVKYKV--DFLVKNSKVLLYQGQYDLRDGVVSTEAWVKKLKW 403
           +  C++ + E  S++   +    +  + + +  +V++Y G  DL     ST A VK++  
Sbjct: 382 WTSCNNELTENWSENDRDTPMIPILHELMGEGVRVMIYNGDVDLEIPFASTLAVVKEMNL 441

Query: 404 EEIERFLEADRKIWRVGDELAGYVQKWK-SLTNVVVLGAGHLLPTDQALNS 428
             ++ F     + W  G +L G+ + +K +LT V V GAGH +PTDQ +++
Sbjct: 442 TVVKEF-----RPWFTGGQLGGFTEDYKGNLTFVTVKGAGHSVPTDQPIHA 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022977756.11.8e-22784.13serine carboxypeptidase-like 50 [Cucurbita maxima][more]
XP_038893258.15.2e-22785.28serine carboxypeptidase-like 50 [Benincasa hispida] >XP_038893259.1 serine carbo... [more]
XP_023534635.11.5e-22684.13serine carboxypeptidase-like 50 isoform X1 [Cucurbita pepo subsp. pepo] >XP_0235... [more]
KAG6600816.12.0e-22683.70Serine carboxypeptidase-like 50, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022941709.19.7e-22683.70serine carboxypeptidase-like 50 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9M9Q67.2e-13957.87Serine carboxypeptidase-like 50 OS=Arabidopsis thaliana OX=3702 GN=SCPL50 PE=2 S... [more]
Q869Q81.9e-6234.41Probable serine carboxypeptidase CPVL OS=Dictyostelium discoideum OX=44689 GN=cp... [more]
Q9H3G51.1e-5933.18Probable serine carboxypeptidase CPVL OS=Homo sapiens OX=9606 GN=CPVL PE=1 SV=2[more]
Q54DY71.9e-5932.25Serine carboxypeptidase S10 family member 1 OS=Dictyostelium discoideum OX=44689... [more]
C9WMM52.5e-5934.71Venom serine carboxypeptidase OS=Apis mellifera OX=7460 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IKV28.6e-22884.13Carboxypeptidase OS=Cucurbita maxima OX=3661 GN=LOC111477961 PE=3 SV=1[more]
A0A6J1FP804.7e-22683.70Carboxypeptidase OS=Cucurbita moschata OX=3662 GN=LOC111446989 PE=3 SV=1[more]
A0A5A7UN233.5e-22182.07Carboxypeptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G001... [more]
A0A1S3C0A86.2e-21883.00Carboxypeptidase OS=Cucumis melo OX=3656 GN=LOC103495166 PE=3 SV=1[more]
A0A6J1D7G24.7e-21080.54Carboxypeptidase OS=Momordica charantia OX=3673 GN=LOC111018332 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G15000.15.1e-14057.87serine carboxypeptidase-like 50 [more]
AT5G22980.18.4e-5029.90serine carboxypeptidase-like 47 [more]
AT3G45010.17.1e-4928.85serine carboxypeptidase-like 48 [more]
AT3G10410.11.6e-4528.96SERINE CARBOXYPEPTIDASE-LIKE 49 [more]
AT3G52010.13.9e-3929.20serine carboxypeptidase-like 37 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 234..259
NoneNo IPR availablePANTHERPTHR11802:SF355CARBOXYPEPTIDASEcoord: 31..443
IPR001563Peptidase S10, serine carboxypeptidasePRINTSPR00724CRBOXYPTASECcoord: 163..188
score: 44.3
coord: 128..138
score: 58.22
coord: 115..127
score: 38.61
coord: 407..420
score: 43.27
IPR001563Peptidase S10, serine carboxypeptidasePFAMPF00450Peptidase_S10coord: 40..435
e-value: 2.5E-98
score: 330.2
IPR001563Peptidase S10, serine carboxypeptidasePANTHERPTHR11802SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASEcoord: 31..443
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 35..448
e-value: 4.5E-99
score: 334.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 39..435
IPR018202Serine carboxypeptidase, serine active sitePROSITEPS00131CARBOXYPEPT_SER_SERcoord: 177..184

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg028734.1Spg028734.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005576 extracellular region
molecular_function GO:0004185 serine-type carboxypeptidase activity