Spg028733 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg028733 vs. NCBI nr
Match: XP_038892860.1 (gibberellin 2-beta-dioxygenase 6 [Benincasa hispida])

HSP 1 Score: 589.0 bits (1517), Expect = 3.0e-164
Identity = 314/384 (81.77%), Postives = 334/384 (86.98%), Query Frame = 0

Query: 1   MASSLDHKQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSRLLNRLPPNLSLPTRRSP 60
           MASSLDH QLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSRLL+RLPPNLSLPTRR P
Sbjct: 16  MASSLDHNQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSRLLHRLPPNLSLPTRRFP 75

Query: 61  SVRSPPTISFSDSPNRDLLDRLLSAATDVGFFQLTDHKISAQLALSAESESAALFDLPAE 120
           SVRSPPTISFS+SPN DLLDRLLSAA+++GFFQLTDHKIS+ LALSAESE+AALFDLPAE
Sbjct: 76  SVRSPPTISFSESPNPDLLDRLLSAASELGFFQLTDHKISSHLALSAESEAAALFDLPAE 135

Query: 121 KKESLFPKNWPLGFKGDGEEESDGVGESFCFDSTLCSSDSAEISLHSLQEFVPAMESLGL 180
           KKESLFPKNWPLG+KGDG+EESDG+GESFCFD   C SDSAEISLHSL+EFV  MESLGL
Sbjct: 136 KKESLFPKNWPLGYKGDGDEESDGIGESFCFDLRPCLSDSAEISLHSLKEFVLKMESLGL 195

Query: 181 KIVEFLFRAIGFENPIGEDRNRFRSLIWISEGSRSAEPVMAGGFYPYIVGLHYQSKNQKC 240
           KI+EFLFRAIGFENPIGEDR  FRSLIWISEG RS EP MA GFYPYIVGL YQSKNQ+C
Sbjct: 196 KILEFLFRAIGFENPIGEDRTGFRSLIWISEGCRSTEPAMADGFYPYIVGLQYQSKNQRC 255

Query: 241 SLLGDSGWVAAAAAA-DSVMVSIGDIAQVWSNGKL-KKVRGRPVPTAATTADANGVNSRT 300
           SLLGDSGWVAAAAAA DSVMVSIGDIAQVWSNGKL KKVRGRPVPTA T    NG NS  
Sbjct: 256 SLLGDSGWVAAAAAATDSVMVSIGDIAQVWSNGKLPKKVRGRPVPTANT----NGANSHA 315

Query: 301 VSISLLITLPADSQVSPLLPTIDENADAEQIGSVTGKTEEEDGNGEGTEEETAAFRWFRF 360
           +S+SLLITLP DSQVSPLL     NAD EQ  SVT K ++ED + +   +E A F  F F
Sbjct: 316 ISLSLLITLPVDSQVSPLLLPSSVNADVEQFRSVTAKKKKEDDDND-ERKEKAMFHSFSF 375

Query: 361 EEYAWRVYHDRYFLLKDPLARYRI 383
           EEYAWRVYHDR FLLKDPL RYRI
Sbjct: 376 EEYAWRVYHDRCFLLKDPLNRYRI 394

BLAST of Spg028733 vs. NCBI nr
Match: XP_011654997.1 (gibberellin 2-beta-dioxygenase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 584.7 bits (1506), Expect = 5.7e-163
Identity = 303/382 (79.32%), Postives = 329/382 (86.13%), Query Frame = 0

Query: 1   MASSLDHKQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSRLLNRLPPNLSLPTRRSP 60
           MASSLDH QLPNIH GATAAPPPTPSSQTNHLSTSAAADALS+LL+RLPPNLSLPTRRSP
Sbjct: 1   MASSLDHNQLPNIHAGATAAPPPTPSSQTNHLSTSAAADALSKLLHRLPPNLSLPTRRSP 60

Query: 61  SVRSPPTISFSDSPNRDLLDRLLSAATDVGFFQLTDHKISAQLALSAESESAALFDLPAE 120
           SV  PPTISFS+SPN DLL+RLLSAA+++GFFQLTDHKIS+ LALSAESESA LF+LPAE
Sbjct: 61  SVIPPPTISFSESPNPDLLNRLLSAASELGFFQLTDHKISSHLALSAESESAPLFNLPAE 120

Query: 121 KKESLFPKNWPLGFKGDGEEESDGVGESFCFDSTLCSSDSAEISLHSLQEFVPAMESLGL 180
           KKESLFPKNWPLGFKGDG+EESDG GES CFDS  C SDS EIS HSL +FV  MESLGL
Sbjct: 121 KKESLFPKNWPLGFKGDGDEESDGSGESLCFDSRNCLSDSPEISFHSLTDFVLEMESLGL 180

Query: 181 KIVEFLFRAIGFENPIGEDRNRFRSLIWISEGSRSAEPVMAGGFYPYIVGLHYQSKNQKC 240
           KIVEFLFRAIGFENPIGEDR  FRSL+WISEG RS EP MAGGFYPYI+GL YQS+NQKC
Sbjct: 181 KIVEFLFRAIGFENPIGEDRTGFRSLVWISEGCRSTEPAMAGGFYPYIIGLQYQSRNQKC 240

Query: 241 SLLGDSGWVAAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVPTAATTADANGVNSRTVS 300
           SLLGDSGWVAAAAAADSVMVSIGDIAQVWSNGKLKK+RGRPVP A++ A+ +  NSRT+S
Sbjct: 241 SLLGDSGWVAAAAAADSVMVSIGDIAQVWSNGKLKKMRGRPVPMASSVANTSSTNSRTIS 300

Query: 301 ISLLITLPADSQVSPLLPTIDENADAEQIGSVTGKTEEEDGNGEGTEEETAAFRWFRFEE 360
           +SLLITLP D+QVSPLL + +ENA+ EQ      K E ED    G  +E A F  F FEE
Sbjct: 301 LSLLITLPVDTQVSPLLLSTNENANEEQF----DKKEREDDGDSGEGKEKAVFHSFNFEE 360

Query: 361 YAWRVYHDRYFLLKDPLARYRI 383
           YAWRVYHDR FLLKDPL RYRI
Sbjct: 361 YAWRVYHDRCFLLKDPLNRYRI 378

BLAST of Spg028733 vs. NCBI nr
Match: XP_008465218.1 (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase [Cucumis melo])

HSP 1 Score: 579.3 bits (1492), Expect = 2.4e-161
Identity = 301/382 (78.80%), Postives = 331/382 (86.65%), Query Frame = 0

Query: 1   MASSLDHKQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSRLLNRLPPNLSLPTRRSP 60
           MASSL+H QLPNIHGGATAAPPPTPSSQTNHLSTSAAADALS+LL+RLPPNLSLPTRRSP
Sbjct: 1   MASSLNHNQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSKLLHRLPPNLSLPTRRSP 60

Query: 61  SVRSPPTISFSDSPNRDLLDRLLSAATDVGFFQLTDHKISAQLALSAESESAALFDLPAE 120
           SV +PPTISFS+SPN DLL+ LLSAA+++GFFQLTDHKIS+ LALSAESESAALF+L AE
Sbjct: 61  SVIAPPTISFSESPNPDLLNHLLSAASELGFFQLTDHKISSHLALSAESESAALFNLSAE 120

Query: 121 KKESLFPKNWPLGFKGDGEEESDGVGESFCFDSTLCSSDSAEISLHSLQEFVPAMESLGL 180
           KKESLFPKNWPLGFKGDG+EESDG G+S CFDS LC SDSAEISLHSL EFV  MESLGL
Sbjct: 121 KKESLFPKNWPLGFKGDGDEESDGSGDSLCFDSRLCFSDSAEISLHSLTEFVLEMESLGL 180

Query: 181 KIVEFLFRAIGFENPIGEDRNRFRSLIWISEGSRSAEPVMAGGFYPYIVGLHYQSKNQKC 240
           KIVEFLFRAIGFENPIGEDR  FRSL+WISEG R  EP MAGGFYPYIVGL YQS+NQ+C
Sbjct: 181 KIVEFLFRAIGFENPIGEDRTGFRSLVWISEGCRGTEPAMAGGFYPYIVGLQYQSRNQRC 240

Query: 241 SLLGDSGWVAAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVPTAATTADANGVNSRTVS 300
           SLLGDSGWVAAAAAADSVMVSIGD+AQVWSNGKLKK+RGRPVP A++ A+ +  NSRT+S
Sbjct: 241 SLLGDSGWVAAAAAADSVMVSIGDVAQVWSNGKLKKMRGRPVPMASSLANTSSTNSRTIS 300

Query: 301 ISLLITLPADSQVSPLLPTIDENADAEQIGSVTGKTEEEDGNGEGTEEETAAFRWFRFEE 360
           +SLLITLP D+QVSPLL T +EN   EQ  SV  K ++E+       +E A F  F FEE
Sbjct: 301 LSLLITLPVDTQVSPLLST-NENEKEEQFASVRDKMKKEEDADNDEGKEKAVFHSFNFEE 360

Query: 361 YAWRVYHDRYFLLKDPLARYRI 383
           YAWRVY DR FLLKDPL RYRI
Sbjct: 361 YAWRVYPDRCFLLKDPLNRYRI 381

BLAST of Spg028733 vs. NCBI nr
Match: XP_022146739.1 (gibberellin 2-beta-dioxygenase 2 [Momordica charantia])

HSP 1 Score: 522.3 bits (1344), Expect = 3.5e-144
Identity = 288/387 (74.42%), Postives = 315/387 (81.40%), Query Frame = 0

Query: 1   MASSLDHKQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSRLLNRLPPNLSLPTRRSP 60
           MASS+DHKQ+     G TAAPPPTPSSQT+HLSTSAAADALSRLL+RLPPNLSLPTRRSP
Sbjct: 1   MASSVDHKQI--YGAGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRSP 60

Query: 61  SVRSPPTISFSDSPNRDLLDRLLSAATDVGFFQLTDHKISAQLALSAESESAALFDLPAE 120
           SVRSPPTISFS++ N  LLDRLLSA++++GFFQLTDH+IS++LALSAESESAALF LP E
Sbjct: 61  SVRSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPME 120

Query: 121 KKESLFPKNWPLGFKGDGEEESDGVGE----SFCFDSTLCSSDSAEISLHSLQEFVPAME 180
            KESLFPKNWPLGFKGDG+E+SDG G     SFC DST CSSDSAEISLHSLQEFV AME
Sbjct: 121 TKESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAME 180

Query: 181 SLGLKIVEFLFRAIGFENPIGEDRNRFRSLIWISEGSRSAEPVMAGGFYPYIVGLHYQSK 240
            LGLKIVEFL RAIGFENPIGEDR RFRSLIWISEGSR AEP +AGGFYPYIVGL YQS+
Sbjct: 181 KLGLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQSR 240

Query: 241 NQKCSLLGDSGWVAAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVPTAATTADANGVNS 300
           NQKCSLLGDS WV AAAAADSVMVSIGDIAQVWSNGKLKKVRGRPV   A  AD NG   
Sbjct: 241 NQKCSLLGDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPV---AVAADPNGGG- 300

Query: 301 RTVSISLLITLPADSQVSPLLPTIDENADAEQIGSVTGKTEEEDGNGEGTEEET-AAFRW 360
             +SISLLITLPA+S+V PL+             +   +++  D   E  EEE   AF+ 
Sbjct: 301 --ISISLLITLPAESEVRPLI-------------TPPARSDRRDEAAEEEEEEVKMAFKS 360

Query: 361 FRFEEYAWRVYHDRYFLLKDPLARYRI 383
           F  EEYAWRV+HDR FLLKDPL RYRI
Sbjct: 361 FWLEEYAWRVHHDRCFLLKDPLDRYRI 366

BLAST of Spg028733 vs. NCBI nr
Match: XP_022943276.1 (gibberellin 3-beta-dioxygenase 4 [Cucurbita moschata] >KAG6599990.1 Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030665.1 Gibberellin 2-beta-dioxygenase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 521.5 bits (1342), Expect = 5.9e-144
Identity = 283/383 (73.89%), Postives = 313/383 (81.72%), Query Frame = 0

Query: 1   MASSLDHKQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSRLLNRLPPNLSLPTRRSP 60
           MASS+DH +LPNI GGATAAPPPTPSSQTNHLSTSAAA+ALSRLL+RLPP LSLPTRRS 
Sbjct: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60

Query: 61  SVRSPPTISFSDSPNRDLLDRLLSAATDVGFFQLTDHKISAQLALSAESESAALFDLPAE 120
           SV SPPTISFSDSP+ +   RLLSA ++ GFFQ+TDHKIS+ L LSAESESAALFD+ AE
Sbjct: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120

Query: 121 KKESLFPKNWPLGFKGDGEEESDGVGESFCFDSTLCSSDSAEISLHSLQEFVPAMESLGL 180
           KKE+LFPK+W  GF GD +EESDG GESFCFD  LC   S EI L +LQE VPAME LGL
Sbjct: 121 KKEALFPKDW--GFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGL 180

Query: 181 KIVEFLFRAIGFENPIGEDRNRFRSLIWISEGSRSAEPVMAGGFYPYIVGLHYQSKNQKC 240
           KIVE LFRAIG ENPIGEDR+RFRSLIWIS+GSRSAEP +  GFYPYIVGLHYQSKNQ+C
Sbjct: 181 KIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRC 240

Query: 241 SLLGDSGWVA-AAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVPTAATTADANGVNSRTV 300
           S+LGDSGWVA AA AADSVMVSIGDIAQVWSNGK+KKVRGR V  AA  +D    NS T+
Sbjct: 241 SVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSD----NSGTI 300

Query: 301 SISLLITLPADSQVSPLLPTIDENADAEQIGSVTGKTEEEDGNGEGTEEETAAFRWFRFE 360
           SISLLITLP D+QVSPLLP  DEN DAE         EE+ G G   E++TA FR F FE
Sbjct: 301 SISLLITLPLDTQVSPLLPPTDENTDAE--------AEEDGGGGNEAEKKTAVFRSFSFE 360

Query: 361 EYAWRVYHDRYFLLKDPLARYRI 383
           EYAW+VY +R FLL+DPLA YRI
Sbjct: 361 EYAWKVYQNRCFLLRDPLAGYRI 369

BLAST of Spg028733 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 4.2e-07
Identity = 69/277 (24.91%), Postives = 117/277 (42.24%), Query Frame = 0

Query: 67  TISFSDSPNRDLLDRLLSAATDVGFFQLTDHKISAQLALSAESESAALFDLPAEKKESLF 126
           +I   D  N D+   +  A+   G FQ+ +H IS +L    ES S  LFD+P+E+K  L 
Sbjct: 48  SIPVIDLSNPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLFDMPSERK--LE 107

Query: 127 PKNWPLGFKGDGEEESDGVGESFCFDSTLCSSDSAEISLHS-------------LQEFVP 186
             +   G  G GE       E   +      +D +  +  +             +QE+V 
Sbjct: 108 AASSDKGVSGYGEPRISPFFEKKMWSEGFTIADDSYRNHFNTLWPHDHTKYCGIIQEYVD 167

Query: 187 AMESLGLKIVEFLFRAIG-------FENPIGEDRNRF-RSLIWISEGSRSAEPVMAGGFY 246
            ME L  +++  +  ++G       + + + +  ++  R  I ++      EP  A G  
Sbjct: 168 EMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHYPVCPEPERAMGLA 227

Query: 247 PY-----IVGLHYQSKNQKCSLLGDSGWVAAAAAADSVMVSIGDIAQVWSNGKLKKVRGR 306
            +     +  LH  +         +SGWV    A   ++V+IGD+  + SNGK       
Sbjct: 228 AHTDSTILTILHQSNTGGLQVFREESGWVTVEPAPGVLVVNIGDLFHILSNGK------- 287

Query: 307 PVPTAATTADANGVNSRTVSISLLITLPA-DSQVSPL 317
            +P+    A  N   SR +SI+ L   PA D Q++P+
Sbjct: 288 -IPSVVHRAKVNHTRSR-ISIAYLWGGPAGDVQIAPI 313

BLAST of Spg028733 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 7.1e-07
Identity = 56/222 (25.23%), Postives = 80/222 (36.04%), Query Frame = 0

Query: 85  AATDVGFFQLTDHKISAQLALSAESESAALFDLPAEKKESLFPKNWPLGFKGDGEEESDG 144
           AA++ GFFQLT+H +  +L      E A LF LP E KE     N    +          
Sbjct: 76  AASEWGFFQLTNHGVGRELMEEMRREQARLFRLPFETKEKAGLLNGSYRWGNPTATSLRH 135

Query: 145 VGESFCFDSTLCSSDSAEISLHSLQEFVPAMESL--GLKIVEFLFRAIGFENPIGEDRNR 204
           +  S  F   L S   A+     L      M+ +   +  V     A   E   G     
Sbjct: 136 LSWSEAFHVPLASISGADCDFGDLTSLRGVMQEVAEAMSRVANTVAAALAEELTGRGGGG 195

Query: 205 FRSLIWISEGSRSAEPVMAGGFYPY-----------------IVGLHYQSKNQKCSLLGD 264
             +  W   G       +    YP                   + +  Q +     L+ D
Sbjct: 196 ASAAPWFPAGCDETTCFLRLNRYPACPFAADTFGLVPHTDSDFLTVLCQDQVGGLHLMKD 255

Query: 265 SGWVAAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVPTAAT 288
           S WVA     D+++V+IGD+ Q WSN + K V  + V  A T
Sbjct: 256 SRWVAVRPRPDALVVNIGDLFQAWSNNRYKSVEHKVVANAKT 297

BLAST of Spg028733 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 5.1e-05
Identity = 68/299 (22.74%), Postives = 122/299 (40.80%), Query Frame = 0

Query: 52  LSLPTRRSPS-VRSPPTISFS------DSPNRDLLDRLLSAATDVGFFQLTDHKISAQLA 111
           LS PT    + VR+   I+FS      D    D    ++ A  D GFF++ +H I     
Sbjct: 4   LSKPTSEQYTYVRNNMPITFSSSIPLVDLSKPDAKTLIVKACEDFGFFKVINHGIPLDAI 63

Query: 112 LSAESESAALFDLPAEKKESLFPKNWPLGFKGDGEEESDGVGESFCFDSTLCSSDSAEIS 171
              ESE+   F LP  +KE   P N P G+       +  +G  +     L ++     S
Sbjct: 64  SQLESEAFKFFSLPQTEKEKAGPAN-PFGYGNKRIGLNGDIG--WIEYLLLTTNQDHNFS 123

Query: 172 LHS---------LQEFVPAMESLGLKIVEFLFRAIGFENPIGEDRNRFRSLIW------- 231
           L+          L+++  AM ++  +I++ +   +       + +N F  L+        
Sbjct: 124 LYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKI-----QPKNVFSKLVMDKQSDCL 183

Query: 232 --------ISEGSRSAEPVMAGGFY--PYIVGLHYQSKNQKCSL-LGDSGWVAAAAAADS 291
                     E + + E ++  G +  P I+ +   +      + L D  W++      S
Sbjct: 184 FRVNHYPACPELAINGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSS 243

Query: 292 VMVSIGDIAQVWSNGKLKKVRGRPVPTAATTADANGVNSRTVSISLLITLPADSQVSPL 317
             +++GD  QV +NG+ K VR R +        ANG++ R +S+      P   +++PL
Sbjct: 244 FFINVGDSLQVMTNGRFKSVRHRVL--------ANGIDPR-LSMIYFCGPPLSEKIAPL 285

BLAST of Spg028733 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 50.1 bits (118), Expect = 6.6e-05
Identity = 71/293 (24.23%), Postives = 116/293 (39.59%), Query Frame = 0

Query: 59  SPSVRSPPTISFSDSPNRDLLDRLLSAATDVGFFQLTDHKISAQLALSAESESAALFDLP 118
           S S  + P I  SD     L+     A T  G FQ+T+H + ++L    E  + +LF LP
Sbjct: 44  SASDETLPLIDLSDIHVATLVGH---ACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLP 103

Query: 119 AEKK------------------ESLFPKN-WPLGFKGDGEEESDGVGESFCFDSTLCSSD 178
            ++K                   S F K  W  GF   G    D       F     S  
Sbjct: 104 VQRKLKAARSENGVSGYGVARIASFFNKKMWSEGFTVIGSPLHD-------FRKLWPSHH 163

Query: 179 SAEISLHSLQEFVPAMESLGLKIVEFLFRAIGFENP------IGEDRNRFRSLIWISEGS 238
                +  ++E+   M+ L  K++ F   ++G E           D    +++I ++   
Sbjct: 164 LKYCEI--IEEYEEHMQKLAAKLMWFALGSLGVEEKDIQWAGPNSDFQGTQAVIQLNHYP 223

Query: 239 RSAEPVMAGGFYPY----IVGLHYQSKNQKCSLLGDS-GWVAAAAAADSVMVSIGDIAQV 298
           +  EP  A G   +    ++ + YQ+      +  D  GWV A     S++V++GD+  +
Sbjct: 224 KCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHI 283

Query: 299 WSNGKLKKVRGRPVPTAATTADANGVNSRTVSISLLITLPADSQVSPLLPTID 322
            +NG          P+    A  N V SR  S++ L   P+D  +SPL   +D
Sbjct: 284 LTNGIF--------PSVLHRARVNHVRSR-FSMAYLWGPPSDIMISPLPKLVD 315

BLAST of Spg028733 vs. ExPASy Swiss-Prot
Match: A0A023GS28 (Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase Diox1 OS=Ruta graveolens OX=37565 GN=DIOX1 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 1.5e-04
Identity = 63/274 (22.99%), Postives = 106/274 (38.69%), Query Frame = 0

Query: 64  SPPTISFSDSPNRDLLDRLLSAATDVGFFQLTDHKISAQLALSAESESAALFDLPAEKKE 123
           S P I  S   + +++  +  AA + GFFQ+ +H +  +     +  +   F LPAE+K 
Sbjct: 65  SIPLIDMSQWDSPEVVKSICDAAENWGFFQIVNHGVPLETLERVKEATHRFFGLPAEEKN 124

Query: 124 SLFPKNWPLGFKGDG-----------------------EEESDGVGESFCFDSTLCSSDS 183
           +   +N P+     G                       EEE++      C D  L    S
Sbjct: 125 NYSKENSPINNVRFGSSFVPHVEKALEWKDFLSMFYVSEEETNTYWPPICRDEMLEYMRS 184

Query: 184 AEISLHSLQEFVPAMESLGLKIVEFLFRAIGFENPIGEDRNRFRSL-------IWISEGS 243
           +E+ +  L E V  ++ L +K ++ +   +     +G  R             + +  G 
Sbjct: 185 SEVLIQRLME-VLVVKGLKVKQIDEIREPM----LVGSRRINLNYYPKCPNPELTLGVGR 244

Query: 244 RSAEPVMAGGFYPYIVGLHYQSKNQKCSLLGDSG--WVAAAAAADSVMVSIGDIAQVWSN 303
            S            I GLH +        L D+G  WV     A S++++IGD  Q+ SN
Sbjct: 245 HSDISTFTILLQDQIGGLHVRK-------LDDTGNTWVHVTPIAGSLIINIGDALQIMSN 304

Query: 304 GKLKKVRGRPVPTAATTADANGVNSRTVSISLLI 306
           G+ K +    V        ANG   R +S+ L +
Sbjct: 305 GRYKSIEHMVV--------ANGTQDR-ISVPLFV 317

BLAST of Spg028733 vs. ExPASy TrEMBL
Match: A0A1S3CNS4 (1-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo OX=3656 GN=LOC103502873 PE=4 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 1.2e-161
Identity = 301/382 (78.80%), Postives = 331/382 (86.65%), Query Frame = 0

Query: 1   MASSLDHKQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSRLLNRLPPNLSLPTRRSP 60
           MASSL+H QLPNIHGGATAAPPPTPSSQTNHLSTSAAADALS+LL+RLPPNLSLPTRRSP
Sbjct: 1   MASSLNHNQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSKLLHRLPPNLSLPTRRSP 60

Query: 61  SVRSPPTISFSDSPNRDLLDRLLSAATDVGFFQLTDHKISAQLALSAESESAALFDLPAE 120
           SV +PPTISFS+SPN DLL+ LLSAA+++GFFQLTDHKIS+ LALSAESESAALF+L AE
Sbjct: 61  SVIAPPTISFSESPNPDLLNHLLSAASELGFFQLTDHKISSHLALSAESESAALFNLSAE 120

Query: 121 KKESLFPKNWPLGFKGDGEEESDGVGESFCFDSTLCSSDSAEISLHSLQEFVPAMESLGL 180
           KKESLFPKNWPLGFKGDG+EESDG G+S CFDS LC SDSAEISLHSL EFV  MESLGL
Sbjct: 121 KKESLFPKNWPLGFKGDGDEESDGSGDSLCFDSRLCFSDSAEISLHSLTEFVLEMESLGL 180

Query: 181 KIVEFLFRAIGFENPIGEDRNRFRSLIWISEGSRSAEPVMAGGFYPYIVGLHYQSKNQKC 240
           KIVEFLFRAIGFENPIGEDR  FRSL+WISEG R  EP MAGGFYPYIVGL YQS+NQ+C
Sbjct: 181 KIVEFLFRAIGFENPIGEDRTGFRSLVWISEGCRGTEPAMAGGFYPYIVGLQYQSRNQRC 240

Query: 241 SLLGDSGWVAAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVPTAATTADANGVNSRTVS 300
           SLLGDSGWVAAAAAADSVMVSIGD+AQVWSNGKLKK+RGRPVP A++ A+ +  NSRT+S
Sbjct: 241 SLLGDSGWVAAAAAADSVMVSIGDVAQVWSNGKLKKMRGRPVPMASSLANTSSTNSRTIS 300

Query: 301 ISLLITLPADSQVSPLLPTIDENADAEQIGSVTGKTEEEDGNGEGTEEETAAFRWFRFEE 360
           +SLLITLP D+QVSPLL T +EN   EQ  SV  K ++E+       +E A F  F FEE
Sbjct: 301 LSLLITLPVDTQVSPLLST-NENEKEEQFASVRDKMKKEEDADNDEGKEKAVFHSFNFEE 360

Query: 361 YAWRVYHDRYFLLKDPLARYRI 383
           YAWRVY DR FLLKDPL RYRI
Sbjct: 361 YAWRVYPDRCFLLKDPLNRYRI 381

BLAST of Spg028733 vs. ExPASy TrEMBL
Match: A0A6J1CYY0 (gibberellin 2-beta-dioxygenase 2 OS=Momordica charantia OX=3673 GN=LOC111015875 PE=4 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 1.7e-144
Identity = 288/387 (74.42%), Postives = 315/387 (81.40%), Query Frame = 0

Query: 1   MASSLDHKQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSRLLNRLPPNLSLPTRRSP 60
           MASS+DHKQ+     G TAAPPPTPSSQT+HLSTSAAADALSRLL+RLPPNLSLPTRRSP
Sbjct: 1   MASSVDHKQI--YGAGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRSP 60

Query: 61  SVRSPPTISFSDSPNRDLLDRLLSAATDVGFFQLTDHKISAQLALSAESESAALFDLPAE 120
           SVRSPPTISFS++ N  LLDRLLSA++++GFFQLTDH+IS++LALSAESESAALF LP E
Sbjct: 61  SVRSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPME 120

Query: 121 KKESLFPKNWPLGFKGDGEEESDGVGE----SFCFDSTLCSSDSAEISLHSLQEFVPAME 180
            KESLFPKNWPLGFKGDG+E+SDG G     SFC DST CSSDSAEISLHSLQEFV AME
Sbjct: 121 TKESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAME 180

Query: 181 SLGLKIVEFLFRAIGFENPIGEDRNRFRSLIWISEGSRSAEPVMAGGFYPYIVGLHYQSK 240
            LGLKIVEFL RAIGFENPIGEDR RFRSLIWISEGSR AEP +AGGFYPYIVGL YQS+
Sbjct: 181 KLGLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQSR 240

Query: 241 NQKCSLLGDSGWVAAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVPTAATTADANGVNS 300
           NQKCSLLGDS WV AAAAADSVMVSIGDIAQVWSNGKLKKVRGRPV   A  AD NG   
Sbjct: 241 NQKCSLLGDSSWVTAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPV---AVAADPNGGG- 300

Query: 301 RTVSISLLITLPADSQVSPLLPTIDENADAEQIGSVTGKTEEEDGNGEGTEEET-AAFRW 360
             +SISLLITLPA+S+V PL+             +   +++  D   E  EEE   AF+ 
Sbjct: 301 --ISISLLITLPAESEVRPLI-------------TPPARSDRRDEAAEEEEEEVKMAFKS 360

Query: 361 FRFEEYAWRVYHDRYFLLKDPLARYRI 383
           F  EEYAWRV+HDR FLLKDPL RYRI
Sbjct: 361 FWLEEYAWRVHHDRCFLLKDPLDRYRI 366

BLAST of Spg028733 vs. ExPASy TrEMBL
Match: A0A6J1FRA7 (gibberellin 3-beta-dioxygenase 4 OS=Cucurbita moschata OX=3662 GN=LOC111448055 PE=4 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.9e-144
Identity = 283/383 (73.89%), Postives = 313/383 (81.72%), Query Frame = 0

Query: 1   MASSLDHKQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSRLLNRLPPNLSLPTRRSP 60
           MASS+DH +LPNI GGATAAPPPTPSSQTNHLSTSAAA+ALSRLL+RLPP LSLPTRRS 
Sbjct: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60

Query: 61  SVRSPPTISFSDSPNRDLLDRLLSAATDVGFFQLTDHKISAQLALSAESESAALFDLPAE 120
           SV SPPTISFSDSP+ +   RLLSA ++ GFFQ+TDHKIS+ L LSAESESAALFD+ AE
Sbjct: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120

Query: 121 KKESLFPKNWPLGFKGDGEEESDGVGESFCFDSTLCSSDSAEISLHSLQEFVPAMESLGL 180
           KKE+LFPK+W  GF GD +EESDG GESFCFD  LC   S EI L +LQE VPAME LGL
Sbjct: 121 KKEALFPKDW--GFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGL 180

Query: 181 KIVEFLFRAIGFENPIGEDRNRFRSLIWISEGSRSAEPVMAGGFYPYIVGLHYQSKNQKC 240
           KIVE LFRAIG ENPIGEDR+RFRSLIWIS+GSRSAEP +  GFYPYIVGLHYQSKNQ+C
Sbjct: 181 KIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRC 240

Query: 241 SLLGDSGWVA-AAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVPTAATTADANGVNSRTV 300
           S+LGDSGWVA AA AADSVMVSIGDIAQVWSNGK+KKVRGR V  AA  +D    NS T+
Sbjct: 241 SVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSD----NSGTI 300

Query: 301 SISLLITLPADSQVSPLLPTIDENADAEQIGSVTGKTEEEDGNGEGTEEETAAFRWFRFE 360
           SISLLITLP D+QVSPLLP  DEN DAE         EE+ G G   E++TA FR F FE
Sbjct: 301 SISLLITLPLDTQVSPLLPPTDENTDAE--------AEEDGGGGNEAEKKTAVFRSFSFE 360

Query: 361 EYAWRVYHDRYFLLKDPLARYRI 383
           EYAW+VY +R FLL+DPLA YRI
Sbjct: 361 EYAWKVYQNRCFLLRDPLAGYRI 369

BLAST of Spg028733 vs. ExPASy TrEMBL
Match: A0A6J1JL54 (uncharacterized protein LOC111485581 OS=Cucurbita maxima OX=3661 GN=LOC111485581 PE=4 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.3e-141
Identity = 280/383 (73.11%), Postives = 310/383 (80.94%), Query Frame = 0

Query: 1   MASSLDHKQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSRLLNRLPPNLSLPTRRSP 60
           MASS+DH +LPNI GGATAAPPPTPSSQTNHLSTSAAA+ALSRLL+RLPP LSLPTRRS 
Sbjct: 103 MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 162

Query: 61  SVRSPPTISFSDSPNRDLLDRLLSAATDVGFFQLTDHKISAQLALSAESESAALFDLPAE 120
           SV SPPTISFS+SP+ +   RLLSA ++ GFFQ+TDHKIS+ L LSA SESAALFD+ AE
Sbjct: 163 SVISPPTISFSESPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAVSESAALFDIQAE 222

Query: 121 KKESLFPKNWPLGFKGDGEEESDGVGESFCFDSTLCSSDSAEISLHSLQEFVPAMESLGL 180
           KKE+LFPK+W  GF GD +EESDG GESFCFD  LC   S EI L +LQE VPAME LGL
Sbjct: 223 KKEALFPKDW--GFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGL 282

Query: 181 KIVEFLFRAIGFENPIGEDRNRFRSLIWISEGSRSAEPVMAGGFYPYIVGLHYQSKNQKC 240
           KIVE LFRAIG ENPIGE R+RFRSLIW S+GSRSAEP +  GFYPYIVGLHYQSKNQ+C
Sbjct: 283 KIVEPLFRAIGLENPIGEGRSRFRSLIWTSKGSRSAEPAVTCGFYPYIVGLHYQSKNQRC 342

Query: 241 SLLGDSGWVA-AAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVPTAATTADANGVNSRTV 300
           S+LGDSGWVA AA AADSVMVSIGDIAQVWSNGK+KKVRGR V  AA  +D    NS T+
Sbjct: 343 SVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSD----NSGTI 402

Query: 301 SISLLITLPADSQVSPLLPTIDENADAEQIGSVTGKTEEEDGNGEGTEEETAAFRWFRFE 360
           SISLLITLP D+QVSPLLP  DEN DAE          EEDG G   E++TA FR F FE
Sbjct: 403 SISLLITLPLDTQVSPLLPPTDENTDAE---------AEEDGGGNEVEKKTAVFRSFSFE 462

Query: 361 EYAWRVYHDRYFLLKDPLARYRI 383
           EYAW+VY +R FLL+DPLA YRI
Sbjct: 463 EYAWKVYQNRCFLLRDPLAGYRI 470

BLAST of Spg028733 vs. ExPASy TrEMBL
Match: A0A5A7UEE7 (1-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold186G001390 PE=4 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 4.3e-124
Identity = 230/267 (86.14%), Postives = 247/267 (92.51%), Query Frame = 0

Query: 1   MASSLDHKQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSRLLNRLPPNLSLPTRRSP 60
           MASSL+H QLPNIHGGATAAPPPTPSSQTNHLSTSAAADALS+LL+RLPPNLSLPTRRSP
Sbjct: 1   MASSLNHNQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSKLLHRLPPNLSLPTRRSP 60

Query: 61  SVRSPPTISFSDSPNRDLLDRLLSAATDVGFFQLTDHKISAQLALSAESESAALFDLPAE 120
           SV +PPTISFS+SPN DLL+ LLSAA+++GFFQLTDHKIS+ LALSAESESAALF+L AE
Sbjct: 61  SVIAPPTISFSESPNPDLLNHLLSAASELGFFQLTDHKISSHLALSAESESAALFNLSAE 120

Query: 121 KKESLFPKNWPLGFKGDGEEESDGVGESFCFDSTLCSSDSAEISLHSLQEFVPAMESLGL 180
           KKESLFPKNWPLGFKGDG+EESDG G+S CFDS LC SDSAEISLHSL EFV  MESLGL
Sbjct: 121 KKESLFPKNWPLGFKGDGDEESDGSGDSLCFDSRLCFSDSAEISLHSLTEFVLEMESLGL 180

Query: 181 KIVEFLFRAIGFENPIGEDRNRFRSLIWISEGSRSAEPVMAGGFYPYIVGLHYQSKNQKC 240
           KIVEFLFRAIGFENPIGEDR  FRSL+WISEG R  EP MAGGFYPYIVGL YQS+NQ+C
Sbjct: 181 KIVEFLFRAIGFENPIGEDRTGFRSLVWISEGCRGTEPAMAGGFYPYIVGLQYQSRNQRC 240

Query: 241 SLLGDSGWVAAAAAADSVMVSIGDIAQ 268
           SLLGDSGWVAAAAAADSVMVSIGD+AQ
Sbjct: 241 SLLGDSGWVAAAAAADSVMVSIGDVAQ 267

BLAST of Spg028733 vs. TAIR 10
Match: AT3G11150.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 282.0 bits (720), Expect = 7.4e-76
Identity = 179/389 (46.02%), Postives = 244/389 (62.72%), Query Frame = 0

Query: 2   ASSLDHKQLPNIHGGATAAPPPTPSSQTNHLSTSA----AADALSRLLNRLPPNLSLPTR 61
           ++ L+H Q    HGGATAAPPPTPS   +HL+T++    A DALS L +RLPP LSLP R
Sbjct: 5   SAHLNHHQPLIKHGGATAAPPPTPS--CSHLNTTSKSETAVDALSSLFHRLPPLLSLPNR 64

Query: 62  RSPSVRSPPTISFSDSPNRDLLDRLLSAATDVGFFQL----TDHKISAQLALSAESESAA 121
           R  S+ S P +S S + +R   D L+SA TD G+FQL    +D      +A +AES+S +
Sbjct: 65  R--SIPSLPMVSLS-AGDRLEWDDLISAVTDFGYFQLINDDSDILFPPGIAEAAESDSLS 124

Query: 122 LFDLPAEKKESLFPKNWPLGFKGDGEEESDGVGESFCFDSTLCSSDSAEISLHSLQEFVP 181
           L +L  EKKES FPK WPLG++ D E        SFC D+  CS+DS+E++L SL+EF  
Sbjct: 125 LLELSEEKKESSFPKKWPLGYEADAET------PSFCLDAD-CSTDSSELNLSSLREFTR 184

Query: 182 AMESLGLKIVEFLFRAIGFENPIGEDRNRFRSLIWISEGSRSAEPVMAGGFYPYIVGLHY 241
            +E +GLK VE L  A+GF    G D  RF +L+W+++G    EP +  GFYP++V L Y
Sbjct: 185 TLEKVGLKTVEMLANALGF----GYDSTRFNTLMWVNQGVPDDEPEVTNGFYPFVVCLQY 244

Query: 242 QSKNQKCSLLGDSGWVAAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVPTAATTADANG 301
           Q + QK  LL +SGWV+     DSV+V++GDIAQVW NG++K+V+ RPV     +   +G
Sbjct: 245 QIREQKYCLLTESGWVSVLPRVDSVLVTLGDIAQVWRNGEVKRVKYRPV---LCSGQKDG 304

Query: 302 VNSRTVSISLLITLPADSQVSPLLPTIDENADAEQIGSVTGKTEEEDGNGEGTEEETAAF 361
              + V+++L++TLP DS VS L   I +    E+        EEE+ +G    +E  AF
Sbjct: 305 -PVKCVTMTLMLTLPMDSMVSSLKDMISDGDKEEEY------AEEEEEDGGARSDERRAF 364

Query: 362 RWFRFEEYAWRVYHDRYFLLKDPLARYRI 383
           R F F+EYAWRVY +R F  +DPL RYRI
Sbjct: 365 RPFSFKEYAWRVYQERLF-FRDPLDRYRI 366

BLAST of Spg028733 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 57.4 bits (137), Expect = 2.9e-08
Identity = 69/277 (24.91%), Postives = 117/277 (42.24%), Query Frame = 0

Query: 67  TISFSDSPNRDLLDRLLSAATDVGFFQLTDHKISAQLALSAESESAALFDLPAEKKESLF 126
           +I   D  N D+   +  A+   G FQ+ +H IS +L    ES S  LFD+P+E+K  L 
Sbjct: 48  SIPVIDLSNPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLFDMPSERK--LE 107

Query: 127 PKNWPLGFKGDGEEESDGVGESFCFDSTLCSSDSAEISLHS-------------LQEFVP 186
             +   G  G GE       E   +      +D +  +  +             +QE+V 
Sbjct: 108 AASSDKGVSGYGEPRISPFFEKKMWSEGFTIADDSYRNHFNTLWPHDHTKYCGIIQEYVD 167

Query: 187 AMESLGLKIVEFLFRAIG-------FENPIGEDRNRF-RSLIWISEGSRSAEPVMAGGFY 246
            ME L  +++  +  ++G       + + + +  ++  R  I ++      EP  A G  
Sbjct: 168 EMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHYPVCPEPERAMGLA 227

Query: 247 PY-----IVGLHYQSKNQKCSLLGDSGWVAAAAAADSVMVSIGDIAQVWSNGKLKKVRGR 306
            +     +  LH  +         +SGWV    A   ++V+IGD+  + SNGK       
Sbjct: 228 AHTDSTILTILHQSNTGGLQVFREESGWVTVEPAPGVLVVNIGDLFHILSNGK------- 287

Query: 307 PVPTAATTADANGVNSRTVSISLLITLPA-DSQVSPL 317
            +P+    A  N   SR +SI+ L   PA D Q++P+
Sbjct: 288 -IPSVVHRAKVNHTRSR-ISIAYLWGGPAGDVQIAPI 313

BLAST of Spg028733 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 54.7 bits (130), Expect = 1.9e-07
Identity = 58/234 (24.79%), Postives = 99/234 (42.31%), Query Frame = 0

Query: 81  RLLSAATDVGFFQLTDHKISAQLALSAESESAALFDLPAEKKESLFPKNWPLGFKGDGEE 140
           +L  A  D GFFQ+ +H I  ++    E  ++  FD+P E+K     K +P+        
Sbjct: 77  KLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPLEEK-----KKYPM-----EPG 136

Query: 141 ESDGVGESFCF--DSTLCSSDSAEISLH-------------------SLQEFVPAMESLG 200
              G G++F F  D  L   +   + +H                   SL+ +   +  L 
Sbjct: 137 TVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSESLEGYSKEIRELC 196

Query: 201 LKIVEFLFRAIG-----FENPIGEDRNRFRSLIWISEGSRSAEPVMAGGFYPYIVG---- 260
            ++++++  ++G     FE   GE     R    ++     + P +  G  P+  G    
Sbjct: 197 KRLLKYIAISLGLKEERFEEMFGEAVQAVR----MNYYPPCSSPDLVLGLSPHSDGSALT 256

Query: 261 LHYQSKNQKCSL--LGDSGWVAAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPV 283
           +  QSKN    L  L D+ WV      ++++++IGD  +V SNGK K V  R V
Sbjct: 257 VLQQSKNSCVGLQILKDNTWVPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAV 296

BLAST of Spg028733 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 50.4 bits (119), Expect = 3.6e-06
Identity = 59/280 (21.07%), Postives = 110/280 (39.29%), Query Frame = 0

Query: 23  PTPSSQTNHLSTSAAADALSRLLNRLPPNLSLPTRRSPSVRSPPTISFSDSPNRDLLDRL 82
           P P +  NH+S              +P    +P   S S+   P ++ +D    +   R+
Sbjct: 5   PQPVTLDNHISL-------------IPTYKPVPVLTSHSI---PVVNLADP---EAKTRI 64

Query: 83  LSAATDVGFFQLTDHKISAQLALSAESESAALFDLPAE-KKESLFPKNWPLGFKGDGEEE 142
           + A  + GFF++ +H +  +L    E E+   F LP   K  +  P+ +  G K  G   
Sbjct: 65  VKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNG 124

Query: 143 SDGVGESFCFD---------STLCSSDSAEISLHSLQEFVPAMESLGLKIVEFLFRAIGF 202
             G  E    +         ++     + +I   S++E++  ++ +  K++E +   +G 
Sbjct: 125 DVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGI 184

Query: 203 ENP-------IGEDRNRFRSLIWISEGSRSAEPVMAGGF----YPYIVGLHYQSKNQKCS 262
           E           E  +    L         AE ++  GF     P I+ +   +      
Sbjct: 185 EPRDTLSKMLRDEKSDSCLRLNHYPAAEEEAEKMVKVGFGEHTDPQIISVLRSNNTAGLQ 244

Query: 263 L-LGDSGWVAAAAAADSVMVSIGDIAQVWSNGKLKKVRGR 281
           + + D  WVA      S  +++GD  QV +NG+ K V+ R
Sbjct: 245 ICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHR 265

BLAST of Spg028733 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 50.1 bits (118), Expect = 4.7e-06
Identity = 71/293 (24.23%), Postives = 116/293 (39.59%), Query Frame = 0

Query: 59  SPSVRSPPTISFSDSPNRDLLDRLLSAATDVGFFQLTDHKISAQLALSAESESAALFDLP 118
           S S  + P I  SD     L+     A T  G FQ+T+H + ++L    E  + +LF LP
Sbjct: 44  SASDETLPLIDLSDIHVATLVGH---ACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLP 103

Query: 119 AEKK------------------ESLFPKN-WPLGFKGDGEEESDGVGESFCFDSTLCSSD 178
            ++K                   S F K  W  GF   G    D       F     S  
Sbjct: 104 VQRKLKAARSENGVSGYGVARIASFFNKKMWSEGFTVIGSPLHD-------FRKLWPSHH 163

Query: 179 SAEISLHSLQEFVPAMESLGLKIVEFLFRAIGFENP------IGEDRNRFRSLIWISEGS 238
                +  ++E+   M+ L  K++ F   ++G E           D    +++I ++   
Sbjct: 164 LKYCEI--IEEYEEHMQKLAAKLMWFALGSLGVEEKDIQWAGPNSDFQGTQAVIQLNHYP 223

Query: 239 RSAEPVMAGGFYPY----IVGLHYQSKNQKCSLLGDS-GWVAAAAAADSVMVSIGDIAQV 298
           +  EP  A G   +    ++ + YQ+      +  D  GWV A     S++V++GD+  +
Sbjct: 224 KCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHI 283

Query: 299 WSNGKLKKVRGRPVPTAATTADANGVNSRTVSISLLITLPADSQVSPLLPTID 322
            +NG          P+    A  N V SR  S++ L   P+D  +SPL   +D
Sbjct: 284 LTNGIF--------PSVLHRARVNHVRSR-FSMAYLWGPPSDIMISPLPKLVD 315