Spg028705 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg028705
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	cucumisin-like
Location	scaffold7: 14018938 .. 14019870 (+)
RNA-Seq Expression	Spg028705
Synteny	Spg028705

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAGCCGGAAACAGTGGCCCAAGACCTTTAACAATTACAAACTTCTCGCCATGGTCACTAGCAGTGGCCGCCAGTACAACTGATAGGAAGTTCTTGACCAGACTTGAACTTGGAGATGGACAAAGTTTCAATGTGAGGCACATTTCTTTTCTACAAATTTGGTTTCAAAGAAACAAAATTTATGAAGCTAATAATATTATCTTTCATTTCTTTTGTGAGAAGGGAGTCTCCATCAATACATTTGATCTCAAAGGAAGACGATATCCATTAGTTTACGCGGGAGATATACCAAATGTTACTACACGCTTCAACCAATCTAGATCGAGGTACTTCAATATTGTGAATTTGCAACAAGAGTAGTACATATATATATCGATGATACATAAATAAAAACAACATTTCTATCGATATGATCGATCTTTTAGAAAAAGACCGAAAGTAAAACCACGCATATATGTAAGTATATATAGCTCAAATAGGAAAATTTTGATCATAATATTATATAAATATGGAACTATCTTCAAATTTTGTCTACTCCCAATACCTGTTGAATACAAAATCGAGATATTTTTACAATTTCTCTCTCTCATTGCATCGAGAACACCTAATAATTTCTCCTTTACAACAACGTTTTTTTTCGGGTGATTACTTCGACCCGTTTAATTTTAATAGTTATGGCTTGTTTTTGTTCTGTGAAATAGGTTATGCTTGGGGAACTCAGTGGACAAGAAGTTGTTGAAGGGTAAAATTGTTGTTTGTGATTCCTTTTTACTTCCAACAAGAGTAGGGGTTTTAGAAGCAGGAGCAGCTGGTGTTATAATGCAAGACCAATCTCCAAAAGACCAAACTTTTTCTCTTATCCCTTGCCTGCTGTTCACATTGCCTCAAACATGGTCCTCTAATTTCCTCTTACATCAATTTAACCCATAG

mRNA sequence

ATGTCAGCCGGAAACAGTGGCCCAAGACCTTTAACAATTACAAACTTCTCGCCATGGTCACTAGCAGTGGCCGCCAGTACAACTGATAGGAAGTTCTTGACCAGACTTGAACTTGGAGATGGACAAAGTTTCAATGGAGTCTCCATCAATACATTTGATCTCAAAGGAAGACGATATCCATTAGTTTACGCGGGAGATATACCAAATGTTACTACACGCTTCAACCAATCTAGATCGAGGTTATGCTTGGGGAACTCAGTGGACAAGAAGTTGTTGAAGGGTAAAATTGTTGTTTGTGATTCCTTTTTACTTCCAACAAGAGTAGGGGTTTTAGAAGCAGGAGCAGCTGGTGTTATAATGCAAGACCAATCTCCAAAAGACCAAACTTTTTCTCTTATCCCTTGCCTGCTGTTCACATTGCCTCAAACATGGTCCTCTAATTTCCTCTTACATCAATTTAACCCATAG

Coding sequence (CDS)

ATGTCAGCCGGAAACAGTGGCCCAAGACCTTTAACAATTACAAACTTCTCGCCATGGTCACTAGCAGTGGCCGCCAGTACAACTGATAGGAAGTTCTTGACCAGACTTGAACTTGGAGATGGACAAAGTTTCAATGGAGTCTCCATCAATACATTTGATCTCAAAGGAAGACGATATCCATTAGTTTACGCGGGAGATATACCAAATGTTACTACACGCTTCAACCAATCTAGATCGAGGTTATGCTTGGGGAACTCAGTGGACAAGAAGTTGTTGAAGGGTAAAATTGTTGTTTGTGATTCCTTTTTACTTCCAACAAGAGTAGGGGTTTTAGAAGCAGGAGCAGCTGGTGTTATAATGCAAGACCAATCTCCAAAAGACCAAACTTTTTCTCTTATCCCTTGCCTGCTGTTCACATTGCCTCAAACATGGTCCTCTAATTTCCTCTTACATCAATTTAACCCATAG

Protein sequence

MSAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPLVYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEAGAAGVIMQDQSPKDQTFSLIPCLLFTLPQTWSSNFLLHQFNP

Homology

[Close]

BLAST of Spg028705 vs. NCBI nr
Match: TYK06003.1 (cucumisin-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 192.6 bits (488), Expect = 2.6e-45
Identity = 92/128 (71.88%), Postives = 109/128 (85.16%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           SAGN GPRP T+TNFSPW+L+VAASTTDR+FLT ++LGDG+ FNGV+INTFDL G +YPL
Sbjct: 253 SAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDLNGTQYPL 312

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEAGAAGVIMQ 121
           VYAG+IPNVT  FN S SR CLGNSVD++L+KGKI VCDS +LP+ VG LE+ A G+IMQ
Sbjct: 313 VYAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLES-ALGIIMQ 372

Query: 122 DQSPKDQT 130
           D SPKD T
Sbjct: 373 DGSPKDLT 379

[Close]

BLAST of Spg028705 vs. NCBI nr
Match: XP_008437521.1 (PREDICTED: cucumisin-like isoform X2 [Cucumis melo])

HSP 1 Score: 190.7 bits (483), Expect = 9.7e-45
Identity = 91/128 (71.09%), Postives = 109/128 (85.16%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           SAGN GPRP T+TNFSPW+L+VAASTTDR+FLT ++LGDG+ FNGV+INTFDL G +YPL
Sbjct: 275 SAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDLNGTQYPL 334

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEAGAAGVIMQ 121
           V+AG+IPNVT  FN S SR CLGNSVD++L+KGKI VCDS +LP+ VG LE+ A G+IMQ
Sbjct: 335 VHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLES-ALGIIMQ 394

Query: 122 DQSPKDQT 130
           D SPKD T
Sbjct: 395 DGSPKDLT 401

[Close]

BLAST of Spg028705 vs. NCBI nr
Match: XP_008437519.1 (PREDICTED: cucumisin-like isoform X1 [Cucumis melo])

HSP 1 Score: 190.7 bits (483), Expect = 9.7e-45
Identity = 91/128 (71.09%), Postives = 109/128 (85.16%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           SAGN GPRP T+TNFSPW+L+VAASTTDR+FLT ++LGDG+ FNGV+INTFDL G +YPL
Sbjct: 308 SAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDLNGTQYPL 367

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEAGAAGVIMQ 121
           V+AG+IPNVT  FN S SR CLGNSVD++L+KGKI VCDS +LP+ VG LE+ A G+IMQ
Sbjct: 368 VHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLES-ALGIIMQ 427

Query: 122 DQSPKDQT 130
           D SPKD T
Sbjct: 428 DGSPKDLT 434

[Close]

BLAST of Spg028705 vs. NCBI nr
Match: XP_008437522.1 (PREDICTED: cucumisin-like isoform X3 [Cucumis melo])

HSP 1 Score: 190.7 bits (483), Expect = 9.7e-45
Identity = 91/128 (71.09%), Postives = 109/128 (85.16%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           SAGN GPRP T+TNFSPW+L+VAASTTDR+FLT ++LGDG+ FNGV+INTFDL G +YPL
Sbjct: 249 SAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDLNGTQYPL 308

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEAGAAGVIMQ 121
           V+AG+IPNVT  FN S SR CLGNSVD++L+KGKI VCDS +LP+ VG LE+ A G+IMQ
Sbjct: 309 VHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLES-ALGIIMQ 368

Query: 122 DQSPKDQT 130
           D SPKD T
Sbjct: 369 DGSPKDLT 375

[Close]

BLAST of Spg028705 vs. NCBI nr
Match: XP_022922127.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 184.9 bits (468), Expect = 5.3e-43
Identity = 90/130 (69.23%), Postives = 108/130 (83.08%), Query Frame = 0


Query: 1   MSAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYP 60
           MSAGN+GP+  T+ NFSPWSL+VAASTTDRKFLT ++LGDG+SFNGV+INTFDL G +YP
Sbjct: 300 MSAGNNGPKSFTVRNFSPWSLSVAASTTDRKFLTGVQLGDGRSFNGVTINTFDLNGTQYP 359

Query: 61  LVYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEAGAAGVIM 120
           LVYAG+IPN+   FN S SR CL NSVDK+ +KGKIV+CD F+LPT +  LE GA G+IM
Sbjct: 360 LVYAGNIPNI--GFNGSISRFCLTNSVDKESVKGKIVLCDFFVLPTNLNFLE-GAIGIIM 419

Query: 121 QDQSPKDQTF 131
           QD +PKD TF
Sbjct: 420 QDNNPKDLTF 426

[Close]

BLAST of Spg028705 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 9.5e-27
Identity = 66/119 (55.46%), Postives = 81/119 (68.07%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           SAGN GP   T  + SPW L+VAAST DRKF+T++++G+GQSF GVSINTFD   + YPL
Sbjct: 304 SAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYPL 363

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEAGAAGVIM 121
           V   DIPN  T F++S SR C   SV+  LLKGKIVVC++   P        GAAGV+M
Sbjct: 364 VSGRDIPN--TGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLM 418

[Close]

BLAST of Spg028705 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 2.0e-24
Identity = 59/129 (45.74%), Postives = 85/129 (65.89%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           SAGN+GP   ++ N SPW + VAAS TDR+F+ R+ LG+G++  G+S+NTF+L G ++P+
Sbjct: 295 SAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPI 354

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEAGAAGVIMQ 121
           VY     NV+   +Q+++  C    VD +L+KGKIV+CD F L  R   L AGA GVI+Q
Sbjct: 355 VYG---QNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDF-LGYREAYL-AGAIGVIVQ 414

Query: 122 DQSPKDQTF 131
           +    D  F
Sbjct: 415 NTLLPDSAF 418

[Close]

BLAST of Spg028705 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 7.8e-21
Identity = 56/132 (42.42%), Postives = 83/132 (62.88%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           +AGN+GP+  T+T+ +PW  +VAAS T+R F+ ++ LGDG+   G S+NT+D+ G  YPL
Sbjct: 304 AAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPL 363

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEA---GAAGV 121
           VY       T   +  ++RLC    +D KL+KGKIV+CDS       G++EA   GA G 
Sbjct: 364 VYGKSAALSTC--SVDKARLCEPKCLDGKLVKGKIVLCDS-----TKGLIEAQKLGAVGS 423

Query: 122 IMQDQSPKDQTF 131
           I+++  P D+ F
Sbjct: 424 IVKNPEP-DRAF 427

[Close]

BLAST of Spg028705 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 1.7e-20
Identity = 51/125 (40.80%), Postives = 82/125 (65.60%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           SAGNSGP+P++++  +PW L VAASTT+R F+T++ LG+G++  G S+N +++KG+ YPL
Sbjct: 296 SAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPL 355

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEA-GAAGVIM 121
           VY       ++  +   + LC  + VDK  +KGKI+VC     P  + ++E+ GA G+I 
Sbjct: 356 VYGKSA--ASSACDAESAGLCELSCVDKSRVKGKILVCGG---PGGLKIVESVGAVGLIY 415

Query: 122 QDQSP 126
           +   P
Sbjct: 416 RTPKP 415

[Close]

BLAST of Spg028705 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 5.0e-20
Identity = 51/126 (40.48%), Postives = 82/126 (65.08%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           SAGNSGP+P T+++ +PW   VAASTT+R F+T++ LG+G++  G S+N FD+KG++YPL
Sbjct: 297 SAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPL 356

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEA-GAAGVIM 121
           VY       ++  +   + LC    ++K  +KGKI+VC     P+   + ++ GA  +I 
Sbjct: 357 VYGKSA--ASSACDAKTAALCAPACLNKSRVKGKILVCGG---PSGYKIAKSVGAIAII- 415

Query: 122 QDQSPK 127
            D+SP+
Sbjct: 417 -DKSPR 415

[Close]

BLAST of Spg028705 vs. ExPASy TrEMBL
Match: A0A5D3C237 (Cucumisin-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001010 PE=3 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 1.2e-45
Identity = 92/128 (71.88%), Postives = 109/128 (85.16%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           SAGN GPRP T+TNFSPW+L+VAASTTDR+FLT ++LGDG+ FNGV+INTFDL G +YPL
Sbjct: 253 SAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDLNGTQYPL 312

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEAGAAGVIMQ 121
           VYAG+IPNVT  FN S SR CLGNSVD++L+KGKI VCDS +LP+ VG LE+ A G+IMQ
Sbjct: 313 VYAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLES-ALGIIMQ 372

Query: 122 DQSPKDQT 130
           D SPKD T
Sbjct: 373 DGSPKDLT 379

[Close]

BLAST of Spg028705 vs. ExPASy TrEMBL
Match: A0A1S3AUT1 (cucumisin-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 4.7e-45
Identity = 91/128 (71.09%), Postives = 109/128 (85.16%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           SAGN GPRP T+TNFSPW+L+VAASTTDR+FLT ++LGDG+ FNGV+INTFDL G +YPL
Sbjct: 249 SAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDLNGTQYPL 308

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEAGAAGVIMQ 121
           V+AG+IPNVT  FN S SR CLGNSVD++L+KGKI VCDS +LP+ VG LE+ A G+IMQ
Sbjct: 309 VHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLES-ALGIIMQ 368

Query: 122 DQSPKDQT 130
           D SPKD T
Sbjct: 369 DGSPKDLT 375

[Close]

BLAST of Spg028705 vs. ExPASy TrEMBL
Match: A0A1S3AUC4 (cucumisin-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 4.7e-45
Identity = 91/128 (71.09%), Postives = 109/128 (85.16%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           SAGN GPRP T+TNFSPW+L+VAASTTDR+FLT ++LGDG+ FNGV+INTFDL G +YPL
Sbjct: 308 SAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDLNGTQYPL 367

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEAGAAGVIMQ 121
           V+AG+IPNVT  FN S SR CLGNSVD++L+KGKI VCDS +LP+ VG LE+ A G+IMQ
Sbjct: 368 VHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLES-ALGIIMQ 427

Query: 122 DQSPKDQT 130
           D SPKD T
Sbjct: 428 DGSPKDLT 434

[Close]

BLAST of Spg028705 vs. ExPASy TrEMBL
Match: A0A1S3AUU4 (cucumisin-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 4.7e-45
Identity = 91/128 (71.09%), Postives = 109/128 (85.16%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           SAGN GPRP T+TNFSPW+L+VAASTTDR+FLT ++LGDG+ FNGV+INTFDL G +YPL
Sbjct: 275 SAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFDLNGTQYPL 334

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEAGAAGVIMQ 121
           V+AG+IPNVT  FN S SR CLGNSVD++L+KGKI VCDS +LP+ VG LE+ A G+IMQ
Sbjct: 335 VHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLES-ALGIIMQ 394

Query: 122 DQSPKDQT 130
           D SPKD T
Sbjct: 395 DGSPKDLT 401

[Close]

BLAST of Spg028705 vs. ExPASy TrEMBL
Match: A0A6J1E2C5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430194 PE=3 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.6e-43
Identity = 90/130 (69.23%), Postives = 108/130 (83.08%), Query Frame = 0


Query: 1   MSAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYP 60
           MSAGN+GP+  T+ NFSPWSL+VAASTTDRKFLT ++LGDG+SFNGV+INTFDL G +YP
Sbjct: 300 MSAGNNGPKSFTVRNFSPWSLSVAASTTDRKFLTGVQLGDGRSFNGVTINTFDLNGTQYP 359

Query: 61  LVYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEAGAAGVIM 120
           LVYAG+IPN+   FN S SR CL NSVDK+ +KGKIV+CD F+LPT +  LE GA G+IM
Sbjct: 360 LVYAGNIPNI--GFNGSISRFCLTNSVDKESVKGKIVLCDFFVLPTNLNFLE-GAIGIIM 419

Query: 121 QDQSPKDQTF 131
           QD +PKD TF
Sbjct: 420 QDNNPKDLTF 426

[Close]

BLAST of Spg028705 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 113.6 bits (283), Expect = 1.4e-25
Identity = 59/129 (45.74%), Postives = 85/129 (65.89%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           SAGN+GP   ++ N SPW + VAAS TDR+F+ R+ LG+G++  G+S+NTF+L G ++P+
Sbjct: 259 SAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPI 318

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEAGAAGVIMQ 121
           VY     NV+   +Q+++  C    VD +L+KGKIV+CD F L  R   L AGA GVI+Q
Sbjct: 319 VYG---QNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDF-LGYREAYL-AGAIGVIVQ 378

Query: 122 DQSPKDQTF 131
           +    D  F
Sbjct: 379 NTLLPDSAF 382

[Close]

BLAST of Spg028705 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 101.7 bits (252), Expect = 5.5e-22
Identity = 56/132 (42.42%), Postives = 83/132 (62.88%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           +AGN+GP+  T+T+ +PW  +VAAS T+R F+ ++ LGDG+   G S+NT+D+ G  YPL
Sbjct: 304 AAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPL 363

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEA---GAAGV 121
           VY       T   +  ++RLC    +D KL+KGKIV+CDS       G++EA   GA G 
Sbjct: 364 VYGKSAALSTC--SVDKARLCEPKCLDGKLVKGKIVLCDS-----TKGLIEAQKLGAVGS 423

Query: 122 IMQDQSPKDQTF 131
           I+++  P D+ F
Sbjct: 424 IVKNPEP-DRAF 427

[Close]

BLAST of Spg028705 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 100.5 bits (249), Expect = 1.2e-21
Identity = 51/125 (40.80%), Postives = 82/125 (65.60%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           SAGNSGP+P++++  +PW L VAASTT+R F+T++ LG+G++  G S+N +++KG+ YPL
Sbjct: 296 SAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPL 355

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEA-GAAGVIM 121
           VY       ++  +   + LC  + VDK  +KGKI+VC     P  + ++E+ GA G+I 
Sbjct: 356 VYGKSA--ASSACDAESAGLCELSCVDKSRVKGKILVCGG---PGGLKIVESVGAVGLIY 415

Query: 122 QDQSP 126
           +   P
Sbjct: 416 RTPKP 415

[Close]

BLAST of Spg028705 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 99.0 bits (245), Expect = 3.6e-21
Identity = 51/126 (40.48%), Postives = 82/126 (65.08%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           SAGNSGP+P T+++ +PW   VAASTT+R F+T++ LG+G++  G S+N FD+KG++YPL
Sbjct: 297 SAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPL 356

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEA-GAAGVIM 121
           VY       ++  +   + LC    ++K  +KGKI+VC     P+   + ++ GA  +I 
Sbjct: 357 VYGKSA--ASSACDAKTAALCAPACLNKSRVKGKILVCGG---PSGYKIAKSVGAIAII- 415

Query: 122 QDQSPK 127
            D+SP+
Sbjct: 417 -DKSPR 415

[Close]

BLAST of Spg028705 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 99.0 bits (245), Expect = 3.6e-21
Identity = 51/126 (40.48%), Postives = 82/126 (65.08%), Query Frame = 0


Query: 2   SAGNSGPRPLTITNFSPWSLAVAASTTDRKFLTRLELGDGQSFNGVSINTFDLKGRRYPL 61
           SAGNSGP+P T+++ +PW   VAASTT+R F+T++ LG+G++  G S+N FD+KG++YPL
Sbjct: 297 SAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPL 356

Query: 62  VYAGDIPNVTTRFNQSRSRLCLGNSVDKKLLKGKIVVCDSFLLPTRVGVLEA-GAAGVIM 121
           VY       ++  +   + LC    ++K  +KGKI+VC     P+   + ++ GA  +I 
Sbjct: 357 VYGKSA--ASSACDAKTAALCAPACLNKSRVKGKILVCGG---PSGYKIAKSVGAIAII- 415

Query: 122 QDQSPK 127
            D+SP+
Sbjct: 417 -DKSPR 415

The following BLAST results are available for this feature:

BLAST of Spg028705 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK06003.1	2.6e-45	71.88	cucumisin-like isoform X1 [Cucumis melo var. makuwa]	[more]
XP_008437521.1	9.7e-45	71.09	PREDICTED: cucumisin-like isoform X2 [Cucumis melo]	[more]
XP_008437519.1	9.7e-45	71.09	PREDICTED: cucumisin-like isoform X1 [Cucumis melo]	[more]
XP_008437522.1	9.7e-45	71.09	PREDICTED: cucumisin-like isoform X3 [Cucumis melo]	[more]
XP_022922127.1	5.3e-43	69.23	cucumisin-like [Cucurbita moschata]	[more]

BLAST of Spg028705 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39547	9.5e-27	55.46	Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
Q9FIF8	2.0e-24	45.74	Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S...	[more]
Q9FGU3	7.8e-21	42.42	Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S...	[more]
Q9FIG2	1.7e-20	40.80	Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2...	[more]
Q8L7D2	5.0e-20	40.48	Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2...	[more]

BLAST of Spg028705 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C237	1.2e-45	71.88	Cucumisin-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3AUT1	4.7e-45	71.09	cucumisin-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1	[more]
A0A1S3AUC4	4.7e-45	71.09	cucumisin-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1	[more]
A0A1S3AUU4	4.7e-45	71.09	cucumisin-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482910 PE=3 SV=1	[more]
A0A6J1E2C5	2.6e-43	69.23	cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111430194 PE=3 SV=1	[more]

BLAST of Spg028705 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59190.1	1.4e-25	45.74	subtilase family protein	[more]
AT5G59100.1	5.5e-22	42.42	Subtilisin-like serine endopeptidase family protein	[more]
AT5G59120.1	1.2e-21	40.80	subtilase 4.13	[more]
AT5G59090.1	3.6e-21	40.48	subtilase 4.12	[more]
AT5G59090.2	3.6e-21	40.48	subtilase 4.12	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.50.30.30		coord: 29..138 e-value: 2.5E-29 score: 104.4
None	No IPR available	PANTHER	PTHR10795:SF375	CUCUMISIN-LIKE	coord: 2..131
None	No IPR available	CDD	cd02120	PA_subtilisin_like	coord: 36..137 e-value: 4.26401E-18 score: 73.2167
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 1..28 e-value: 2.5E-29 score: 104.4
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 2..64
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 2..131

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg028705.1	Spg028705.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity