Spg028694 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg028694 vs. NCBI nr
Match: KAA0044449.1 (hypothetical protein E6C27_scaffold46G001820 [Cucumis melo var. makuwa])

HSP 1 Score: 229.9 bits (585), Expect = 6.7e-56
Identity = 197/736 (26.77%), Postives = 301/736 (40.90%), Query Frame = 0

Query: 90  DLSTNTHQP-------PAPTKSTPKSQFATLYLSSAVIVQRKNFHDSWYDIMRAL--QMD 149
           D ST++  P        + + S+  S+       S   ++R+ FHD W  I+  L  Q D
Sbjct: 157 DSSTDSESPRKTYAEAVSSSSSSSSSESDCSKTKSTSSLKRRCFHDDWAKIIDRLRDQTD 216

Query: 150 L--SAFSSISPLQPDKALLACEDEEQARTVAKIKGWYKVGIFQVRFFPWSVAAMIGEPKV 209
              S F  I P   DKALL  +D+E A+ + K  GW  VG F V+F  WS  A      +
Sbjct: 217 KKDSCFRYI-PFHADKALLFIKDKELAKLLCKNIGWTTVGPFYVKFEKWSKNAHADTKVI 276

Query: 210 PSYGGWIKVRNLPLDKWSIDTFKKIGDECGGYLKTASKTLSRMDMMEIGIRVKENCSA-- 269
           PSYGGW + R +PL  W+++TF +IG+  GG++  A ++++++++ E  I+VKEN +   
Sbjct: 277 PSYGGWTRFRGIPLHIWNLNTFIQIGEAYGGFIDAAPESVNKLELTEALIKVKENYTGFL 336

Query: 270 --------------------------------GIHGRIPK-------------------- 329
                                            IHG   K                    
Sbjct: 337 PAFIQIHDEEGHDFIIQTVTHPKGKWLRERNPSIHGSFTKTAAENFNEFNPYAEQYTFRR 396

Query: 330 ----------PETN------ANNPRAV--------------------------------- 389
                     PE++       NN R +                                 
Sbjct: 397 NLAVIAKPDLPESSKKGSKQMNNDRKMGPTKTIKKFNKTVSFLNLNYEGDSDNSNSSEKI 456

Query: 390 ----HEDVREEKRRGDHPRVVVLGQQVNPYKAETQ---GTPLDALFT-STSLTPATIPEV 449
               H     EK++G         Q+++P   + +    +P +  F  S    P    ++
Sbjct: 457 QKKDHRSEMTEKKKGKQICCGEYNQKISPINTKRKVSFSSPKNETFLFSAHSAPTKTLKL 516

Query: 450 GPLLSLPIREPQSPKPTPTSQDGPHPSNITNIDSEGAWS--------------------- 509
           G  +S    E +S +PT + +   +     +++ E + +                     
Sbjct: 517 GSPMSYAKMEAKSKRPTQSIKKKVYRVKSRSMERETSQTSRQKDKGKIDPNEFELVVDLG 576

Query: 510 -----SPCTAAMEDSPT--PSLSKPTIHSISPPTITKLFETQYEPDPQPLLEDHVPLRIE 569
                S    +  +SP+  PS + PT   I   ++  +    +E D +   ++++    E
Sbjct: 577 HISLLSDTDFSCPESPSYIPSPTSPTESDIVKDSLASMMTCAHE-DREKKKKENIREETE 636

Query: 570 DP------------YHQNLQ-------QNRNLEESTLIDIINVEKAETGEWEEEESEIDP 629
           D                NL+       Q  ++    +I I+N      G     E  ID 
Sbjct: 637 DDEVSFKRKLTDWLKENNLRLAADFNSQFNSVTNDRMISILN-GPPNVGVENVSEDVIDG 696

Query: 630 AVYLPIIFP-------WLTEHGVYGPNTSRDRKYFWRELADLQSLCLPHWILGGDFNVTR 650
           A  + I          WL+   +YGP   ++R  FW EL +L+S+C P WILGGDFNV R
Sbjct: 697 AFSVSIQVDSNNGASWWLS--AIYGPAKRKNRPLFWEELENLKSICFPTWILGGDFNVIR 756

BLAST of Spg028694 vs. NCBI nr
Match: XP_022158956.1 (uncharacterized protein LOC111025405 [Momordica charantia])

HSP 1 Score: 229.6 bits (584), Expect = 8.8e-56
Identity = 106/242 (43.80%), Postives = 147/242 (60.74%), Query Frame = 0

Query: 475 GVYGPNTSRDRKYFWRELADLQSLCLPHWILGGDFNVTRWSWEKSTFTAPSRAMRRFNRF 534
           G+YGP+T+     FW+EL DL  LC  HWIL GDFNVTRWSWEKS     +++M  FN F
Sbjct: 107 GIYGPSTTEFHYLFWQELLDLSDLCENHWILAGDFNVTRWSWEKSNGRPLTKSMWLFNSF 166

Query: 535 IDCAELQDIPLTNGKYTWSNFRPSPTMTLIDRFLISEHIPIKFGATTTRRLDRVTSDHFP 594
           I+ + L D+PLTNG++TWS    + + +LID FL++     K G    +R+ R TSDHFP
Sbjct: 167 IEDSSLIDVPLTNGQHTWSR---NTSFSLIDCFLLTNGCIDKLGMPIAKRMTRTTSDHFP 226

Query: 595 ISLSLGNDRWGPTPFRFINVWLTHHSFLHTVDSWWKNTPFRGWPGHGFIQKLKGLKLELK 654
           I L  G + WG TPFRF N+WL+H +F   +++WW N P  GWPGHG + KLK LK  +K
Sbjct: 227 ILLDFGQNNWGLTPFRFENMWLSHKTFKPFLETWWGNKPLHGWPGHGLMMKLKSLKYAIK 286

Query: 655 QWNQNVFGNQKEQKTRLGQELLAIDNREENGHLSEQDPSRRTEIRAALITISAKEEIMWR 714
            W    F     QK  L   + ++D+ E +  ++      R + +  L+++ AKEE  WR
Sbjct: 287 LWITEHFRCIHSQKEDLTNLMNSLDDLEGSQPVTPDQSRARIQAKEDLLSVVAKEEAFWR 345

Query: 715 SK 717
            +
Sbjct: 347 QR 345

BLAST of Spg028694 vs. NCBI nr
Match: CAN66330.1 (hypothetical protein VITISV_000598 [Vitis vinifera])

HSP 1 Score: 219.2 bits (557), Expect = 1.2e-52
Identity = 107/244 (43.85%), Postives = 141/244 (57.79%), Query Frame = 0

Query: 475  GVYGPNTSRDRKYFWRELADLQSLCLPHWILGGDFNVTRWSWEKSTFTAPSRAMRRFNRF 534
            GVYGPN    RK FW EL+DL  L  P W +GGDFNV R   EK   +  + +MR F+ F
Sbjct: 1628 GVYGPNNPSIRKEFWXELSDLFGLTYPSWCVGGDFNVIRRRSEKLGGSRVTSSMRDFDGF 1687

Query: 535  IDCAELQDIPLTNGKYTWSNFRPSPTMTLIDRFLISEHIPIKFGATTTRRLDRVTSDHFP 594
            I  +EL D PL N  +TWSN + SP    +DRFL S    + F  +    L R TSDH+P
Sbjct: 1688 IRESELHDSPLRNASFTWSNMQESPVCKRLDRFLYSNEWXLSFPQSLQEVLPRWTSDHWP 1747

Query: 595  ISLSLGNDRWGPTPFRFINVWLTHHSFLHTVDSWWKNTPFRGWPGHGFIQKLKGLKLELK 654
            I L     +WGPTPFRF N+WL HH+F  +  SWW+     GW GH F++KL+ +K +LK
Sbjct: 1748 IVLDTNPFKWGPTPFRFENMWLQHHNFKESFSSWWREFEGNGWEGHKFMRKLQFVKAKLK 1807

Query: 655  QWNQNVFGNQKEQKTRLGQELLAIDNREENGHLSEQDPSRRTEIRAALITISAKEEIMWR 714
             WN+N FG  KE+K  +  E+  ID  E+ G LS    + R   +  L  +  +EEI W+
Sbjct: 1808 DWNKNTFGLLKERKKSISDEIANIDAIEQEGALSSDLVALRAIRKGELEELILREEIXWK 1867

Query: 715  SKVQ 719
             K +
Sbjct: 1868 QKAK 1871

BLAST of Spg028694 vs. NCBI nr
Match: KAA0056838.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYJ99341.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 218.4 bits (555), Expect = 2.0e-52
Identity = 214/833 (25.69%), Postives = 309/833 (37.09%), Query Frame = 0

Query: 79  SYKEALKKKQRDLSTNTHQPPAPTKSTPKSQ---FATLYLS-----SAVIVQRKNFHDSW 138
           SY + L     D +   ++  +   S+ +S    F    LS       VI+ R+ FHD W
Sbjct: 135 SYAKVLSDSSEDDNKKRYKATSDDSSSRRSSSIGFKPFTLSGNSFEKTVIITRRCFHDDW 194

Query: 139 YDIMRALQMDLSAFSSISPLQPDKALLACEDEEQARTVAKIK---GWYKVGIFQVRFFPW 198
             IM +L+       S  P Q DKA+L   + + A+ +   K   GW  VG +QV+F  W
Sbjct: 195 NRIMFSLRKQSEIAFSYKPFQADKAILFL-NSDHAKLLCSNKGANGWSTVGNYQVKFESW 254

Query: 199 SVAAMIGEPKVPSYGGWIKVRNLPLDKWSIDTFKKIGDECGGYLKTASKTLSRMDMMEIG 258
                     +PSYGGW++ R +PL  W+ +TF+ IG  CGG+L  A +T+    +++  
Sbjct: 255 DSNLHSFHSVIPSYGGWLRFRGIPLHLWNYNTFQHIGSACGGFLDVAKETMQMDKLIDAK 314

Query: 259 IRVKENCSAGIHGRIPKPETNANN-----------------PRAVHEDVR----EEKRRG 318
           I+V+ N +  +   I   +    N                    VH   R    +E  + 
Sbjct: 315 IKVRYNYTGFVPASILITDNQGENFIVTTVQPAEARWLVERNVRVHGSFRTKAADEFDQH 374

Query: 319 DH--PRVVVLGQQVNP---------YKAETQGTPLDALFTSTSLTPATIPEVGPL-LSLP 378
           +H        G Q  P         Y          +  T      ++  E  P    L 
Sbjct: 375 NHLAETYTYNGFQAIPPEPTRTHGDYSIHNSDKHSISYHTQAKKNNSSESEYDPFDQQLS 434

Query: 379 IREPQSPKPTPTSQDGPH------PSNITNIDSEGAWSSPCTAAMEDSPTPSLSKPTIHS 438
            R  +  K      D  H         I+N   + ++ SP       S T   +K     
Sbjct: 435 DRRKEKGKAILIINDQNHGHYSKRSKRISN--RKVSFLSPGGIQSNSSNTEINTKGKSLE 494

Query: 439 ISPPTITKLFETQYEP--------------DPQPLLEDH-----------------VPLR 498
           IS  TI   FE ++ P              DPQ   EDH                 V + 
Sbjct: 495 IS--TINDQFEKRWSPRQKTKTKLTYRIKKDPQESTEDHNLSLKETGEGSKQMNLSVDMG 554

Query: 499 IEDPYHQNLQ---------------------------------------QNRNLEESTLI 558
              P    +Q                                       QN++   ST  
Sbjct: 555 PISPLESMIQSENNHGLDTLNNQTPDGNSKSTDSAEAKNLTVSVKEGADQNKSASRSTAE 614

Query: 559 DIINVEKAETGE--------------W-EEEESEIDP--------AVYLPIIFP------ 618
              N + A+TG               W +E E ++ P        +   P+I        
Sbjct: 615 G--NSKDAKTGSEMEIDRAFKEKLVIWLKENELKLSPKYTNDVPSSSSFPVIVSDQNMDI 674

Query: 619 --------------------------------------------WLTEHGVYGPNTSRDR 678
                                                       WLT   VYGP    DR
Sbjct: 675 AGHGPLGDKGGILVLWDDTKFKVNDIKVGNYSISLNILNTNGNWWLT--SVYGPYKYNDR 734

Query: 679 KYFWRELADLQSLCLPHWILGGDFNVTRWSWEKSTFTAPSRAMRRFNRFIDCAELQDIPL 719
              W EL  LQSLCLP+W++ GDFN+ RW  E +  +   R M  FN FI   EL D PL
Sbjct: 735 TKLWPELEILQSLCLPNWLIAGDFNIVRWERETNAKSLDKRNMANFNNFISVNELIDPPL 794

BLAST of Spg028694 vs. NCBI nr
Match: RVW77758.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 217.2 bits (552), Expect = 4.5e-52
Identity = 107/249 (42.97%), Postives = 143/249 (57.43%), Query Frame = 0

Query: 470 WLTEHGVYGPNTSRDRKYFWRELADLQSLCLPHWILGGDFNVTRWSWEKSTFTAPSRAMR 529
           W++   VYGPN    RK FW EL+DL  L  P W +GGDFNV R   EK   +  + +MR
Sbjct: 212 WMS--AVYGPNNPSIRKEFWAELSDLFGLTYPSWCVGGDFNVIRRRSEKLGGSRVTSSMR 271

Query: 530 RFNRFIDCAELQDIPLTNGKYTWSNFRPSPTMTLIDRFLISEHIPIKFGATTTRRLDRVT 589
            F+ FI  +EL D PL N  +TWSN + SP    +DRFL S    + F  +    L R T
Sbjct: 272 DFDGFIRESELHDSPLRNASFTWSNMQESPVCKRLDRFLYSNEWKLSFPQSLQEVLPRWT 331

Query: 590 SDHFPISLSLGNDRWGPTPFRFINVWLTHHSFLHTVDSWWKNTPFRGWPGHGFIQKLKGL 649
           SDH+PI L     +WGPTPFRF N+WL HH+F  +  SWW+     GW GH F++KL+ +
Sbjct: 332 SDHWPIVLDTNPFKWGPTPFRFENMWLQHHNFKESFSSWWREFEGNGWEGHKFMRKLQFV 391

Query: 650 KLELKQWNQNVFGNQKEQKTRLGQELLAIDNREENGHLSEQDPSRRTEIRAALITISAKE 709
           K +LK WN+N FG  KE+K  +  E+  ID  E+ G LS    + R   +  L  +  +E
Sbjct: 392 KAKLKDWNKNTFGLLKERKKSISDEIANIDAIEQEGALSSDLVALRAIRKGELEELILRE 451

Query: 710 EIMWRSKVQ 719
           EI W+ K +
Sbjct: 452 EIHWKQKAK 458

BLAST of Spg028694 vs. ExPASy TrEMBL
Match: A0A5A7TTA1 (DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G001820 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 3.2e-56
Identity = 197/736 (26.77%), Postives = 301/736 (40.90%), Query Frame = 0

Query: 90  DLSTNTHQP-------PAPTKSTPKSQFATLYLSSAVIVQRKNFHDSWYDIMRAL--QMD 149
           D ST++  P        + + S+  S+       S   ++R+ FHD W  I+  L  Q D
Sbjct: 157 DSSTDSESPRKTYAEAVSSSSSSSSSESDCSKTKSTSSLKRRCFHDDWAKIIDRLRDQTD 216

Query: 150 L--SAFSSISPLQPDKALLACEDEEQARTVAKIKGWYKVGIFQVRFFPWSVAAMIGEPKV 209
              S F  I P   DKALL  +D+E A+ + K  GW  VG F V+F  WS  A      +
Sbjct: 217 KKDSCFRYI-PFHADKALLFIKDKELAKLLCKNIGWTTVGPFYVKFEKWSKNAHADTKVI 276

Query: 210 PSYGGWIKVRNLPLDKWSIDTFKKIGDECGGYLKTASKTLSRMDMMEIGIRVKENCSA-- 269
           PSYGGW + R +PL  W+++TF +IG+  GG++  A ++++++++ E  I+VKEN +   
Sbjct: 277 PSYGGWTRFRGIPLHIWNLNTFIQIGEAYGGFIDAAPESVNKLELTEALIKVKENYTGFL 336

Query: 270 --------------------------------GIHGRIPK-------------------- 329
                                            IHG   K                    
Sbjct: 337 PAFIQIHDEEGHDFIIQTVTHPKGKWLRERNPSIHGSFTKTAAENFNEFNPYAEQYTFRR 396

Query: 330 ----------PETN------ANNPRAV--------------------------------- 389
                     PE++       NN R +                                 
Sbjct: 397 NLAVIAKPDLPESSKKGSKQMNNDRKMGPTKTIKKFNKTVSFLNLNYEGDSDNSNSSEKI 456

Query: 390 ----HEDVREEKRRGDHPRVVVLGQQVNPYKAETQ---GTPLDALFT-STSLTPATIPEV 449
               H     EK++G         Q+++P   + +    +P +  F  S    P    ++
Sbjct: 457 QKKDHRSEMTEKKKGKQICCGEYNQKISPINTKRKVSFSSPKNETFLFSAHSAPTKTLKL 516

Query: 450 GPLLSLPIREPQSPKPTPTSQDGPHPSNITNIDSEGAWS--------------------- 509
           G  +S    E +S +PT + +   +     +++ E + +                     
Sbjct: 517 GSPMSYAKMEAKSKRPTQSIKKKVYRVKSRSMERETSQTSRQKDKGKIDPNEFELVVDLG 576

Query: 510 -----SPCTAAMEDSPT--PSLSKPTIHSISPPTITKLFETQYEPDPQPLLEDHVPLRIE 569
                S    +  +SP+  PS + PT   I   ++  +    +E D +   ++++    E
Sbjct: 577 HISLLSDTDFSCPESPSYIPSPTSPTESDIVKDSLASMMTCAHE-DREKKKKENIREETE 636

Query: 570 DP------------YHQNLQ-------QNRNLEESTLIDIINVEKAETGEWEEEESEIDP 629
           D                NL+       Q  ++    +I I+N      G     E  ID 
Sbjct: 637 DDEVSFKRKLTDWLKENNLRLAADFNSQFNSVTNDRMISILN-GPPNVGVENVSEDVIDG 696

Query: 630 AVYLPIIFP-------WLTEHGVYGPNTSRDRKYFWRELADLQSLCLPHWILGGDFNVTR 650
           A  + I          WL+   +YGP   ++R  FW EL +L+S+C P WILGGDFNV R
Sbjct: 697 AFSVSIQVDSNNGASWWLS--AIYGPAKRKNRPLFWEELENLKSICFPTWILGGDFNVIR 756

BLAST of Spg028694 vs. ExPASy TrEMBL
Match: A0A6J1E2G6 (uncharacterized protein LOC111025405 OS=Momordica charantia OX=3673 GN=LOC111025405 PE=4 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 4.2e-56
Identity = 106/242 (43.80%), Postives = 147/242 (60.74%), Query Frame = 0

Query: 475 GVYGPNTSRDRKYFWRELADLQSLCLPHWILGGDFNVTRWSWEKSTFTAPSRAMRRFNRF 534
           G+YGP+T+     FW+EL DL  LC  HWIL GDFNVTRWSWEKS     +++M  FN F
Sbjct: 107 GIYGPSTTEFHYLFWQELLDLSDLCENHWILAGDFNVTRWSWEKSNGRPLTKSMWLFNSF 166

Query: 535 IDCAELQDIPLTNGKYTWSNFRPSPTMTLIDRFLISEHIPIKFGATTTRRLDRVTSDHFP 594
           I+ + L D+PLTNG++TWS    + + +LID FL++     K G    +R+ R TSDHFP
Sbjct: 167 IEDSSLIDVPLTNGQHTWSR---NTSFSLIDCFLLTNGCIDKLGMPIAKRMTRTTSDHFP 226

Query: 595 ISLSLGNDRWGPTPFRFINVWLTHHSFLHTVDSWWKNTPFRGWPGHGFIQKLKGLKLELK 654
           I L  G + WG TPFRF N+WL+H +F   +++WW N P  GWPGHG + KLK LK  +K
Sbjct: 227 ILLDFGQNNWGLTPFRFENMWLSHKTFKPFLETWWGNKPLHGWPGHGLMMKLKSLKYAIK 286

Query: 655 QWNQNVFGNQKEQKTRLGQELLAIDNREENGHLSEQDPSRRTEIRAALITISAKEEIMWR 714
            W    F     QK  L   + ++D+ E +  ++      R + +  L+++ AKEE  WR
Sbjct: 287 LWITEHFRCIHSQKEDLTNLMNSLDDLEGSQPVTPDQSRARIQAKEDLLSVVAKEEAFWR 345

Query: 715 SK 717
            +
Sbjct: 347 QR 345

BLAST of Spg028694 vs. ExPASy TrEMBL
Match: A5C3T9 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_000598 PE=4 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 5.7e-53
Identity = 107/244 (43.85%), Postives = 141/244 (57.79%), Query Frame = 0

Query: 475  GVYGPNTSRDRKYFWRELADLQSLCLPHWILGGDFNVTRWSWEKSTFTAPSRAMRRFNRF 534
            GVYGPN    RK FW EL+DL  L  P W +GGDFNV R   EK   +  + +MR F+ F
Sbjct: 1628 GVYGPNNPSIRKEFWXELSDLFGLTYPSWCVGGDFNVIRRRSEKLGGSRVTSSMRDFDGF 1687

Query: 535  IDCAELQDIPLTNGKYTWSNFRPSPTMTLIDRFLISEHIPIKFGATTTRRLDRVTSDHFP 594
            I  +EL D PL N  +TWSN + SP    +DRFL S    + F  +    L R TSDH+P
Sbjct: 1688 IRESELHDSPLRNASFTWSNMQESPVCKRLDRFLYSNEWXLSFPQSLQEVLPRWTSDHWP 1747

Query: 595  ISLSLGNDRWGPTPFRFINVWLTHHSFLHTVDSWWKNTPFRGWPGHGFIQKLKGLKLELK 654
            I L     +WGPTPFRF N+WL HH+F  +  SWW+     GW GH F++KL+ +K +LK
Sbjct: 1748 IVLDTNPFKWGPTPFRFENMWLQHHNFKESFSSWWREFEGNGWEGHKFMRKLQFVKAKLK 1807

Query: 655  QWNQNVFGNQKEQKTRLGQELLAIDNREENGHLSEQDPSRRTEIRAALITISAKEEIMWR 714
             WN+N FG  KE+K  +  E+  ID  E+ G LS    + R   +  L  +  +EEI W+
Sbjct: 1808 DWNKNTFGLLKERKKSISDEIANIDAIEQEGALSSDLVALRAIRKGELEELILREEIXWK 1867

Query: 715  SKVQ 719
             K +
Sbjct: 1868 QKAK 1871

BLAST of Spg028694 vs. ExPASy TrEMBL
Match: A0A5D3BKT8 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005570 PE=4 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 9.8e-53
Identity = 214/833 (25.69%), Postives = 309/833 (37.09%), Query Frame = 0

Query: 79  SYKEALKKKQRDLSTNTHQPPAPTKSTPKSQ---FATLYLS-----SAVIVQRKNFHDSW 138
           SY + L     D +   ++  +   S+ +S    F    LS       VI+ R+ FHD W
Sbjct: 135 SYAKVLSDSSEDDNKKRYKATSDDSSSRRSSSIGFKPFTLSGNSFEKTVIITRRCFHDDW 194

Query: 139 YDIMRALQMDLSAFSSISPLQPDKALLACEDEEQARTVAKIK---GWYKVGIFQVRFFPW 198
             IM +L+       S  P Q DKA+L   + + A+ +   K   GW  VG +QV+F  W
Sbjct: 195 NRIMFSLRKQSEIAFSYKPFQADKAILFL-NSDHAKLLCSNKGANGWSTVGNYQVKFESW 254

Query: 199 SVAAMIGEPKVPSYGGWIKVRNLPLDKWSIDTFKKIGDECGGYLKTASKTLSRMDMMEIG 258
                     +PSYGGW++ R +PL  W+ +TF+ IG  CGG+L  A +T+    +++  
Sbjct: 255 DSNLHSFHSVIPSYGGWLRFRGIPLHLWNYNTFQHIGSACGGFLDVAKETMQMDKLIDAK 314

Query: 259 IRVKENCSAGIHGRIPKPETNANN-----------------PRAVHEDVR----EEKRRG 318
           I+V+ N +  +   I   +    N                    VH   R    +E  + 
Sbjct: 315 IKVRYNYTGFVPASILITDNQGENFIVTTVQPAEARWLVERNVRVHGSFRTKAADEFDQH 374

Query: 319 DH--PRVVVLGQQVNP---------YKAETQGTPLDALFTSTSLTPATIPEVGPL-LSLP 378
           +H        G Q  P         Y          +  T      ++  E  P    L 
Sbjct: 375 NHLAETYTYNGFQAIPPEPTRTHGDYSIHNSDKHSISYHTQAKKNNSSESEYDPFDQQLS 434

Query: 379 IREPQSPKPTPTSQDGPH------PSNITNIDSEGAWSSPCTAAMEDSPTPSLSKPTIHS 438
            R  +  K      D  H         I+N   + ++ SP       S T   +K     
Sbjct: 435 DRRKEKGKAILIINDQNHGHYSKRSKRISN--RKVSFLSPGGIQSNSSNTEINTKGKSLE 494

Query: 439 ISPPTITKLFETQYEP--------------DPQPLLEDH-----------------VPLR 498
           IS  TI   FE ++ P              DPQ   EDH                 V + 
Sbjct: 495 IS--TINDQFEKRWSPRQKTKTKLTYRIKKDPQESTEDHNLSLKETGEGSKQMNLSVDMG 554

Query: 499 IEDPYHQNLQ---------------------------------------QNRNLEESTLI 558
              P    +Q                                       QN++   ST  
Sbjct: 555 PISPLESMIQSENNHGLDTLNNQTPDGNSKSTDSAEAKNLTVSVKEGADQNKSASRSTAE 614

Query: 559 DIINVEKAETGE--------------W-EEEESEIDP--------AVYLPIIFP------ 618
              N + A+TG               W +E E ++ P        +   P+I        
Sbjct: 615 G--NSKDAKTGSEMEIDRAFKEKLVIWLKENELKLSPKYTNDVPSSSSFPVIVSDQNMDI 674

Query: 619 --------------------------------------------WLTEHGVYGPNTSRDR 678
                                                       WLT   VYGP    DR
Sbjct: 675 AGHGPLGDKGGILVLWDDTKFKVNDIKVGNYSISLNILNTNGNWWLT--SVYGPYKYNDR 734

Query: 679 KYFWRELADLQSLCLPHWILGGDFNVTRWSWEKSTFTAPSRAMRRFNRFIDCAELQDIPL 719
              W EL  LQSLCLP+W++ GDFN+ RW  E +  +   R M  FN FI   EL D PL
Sbjct: 735 TKLWPELEILQSLCLPNWLIAGDFNIVRWERETNAKSLDKRNMANFNNFISVNELIDPPL 794

BLAST of Spg028694 vs. ExPASy TrEMBL
Match: A0A438GZW0 (LINE-1 retrotransposable element ORF2 protein OS=Vitis vinifera OX=29760 GN=Pol_7 PE=4 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.2e-52
Identity = 107/249 (42.97%), Postives = 143/249 (57.43%), Query Frame = 0

Query: 470 WLTEHGVYGPNTSRDRKYFWRELADLQSLCLPHWILGGDFNVTRWSWEKSTFTAPSRAMR 529
           W++   VYGPN    RK FW EL+DL  L  P W +GGDFNV R   EK   +  + +MR
Sbjct: 212 WMS--AVYGPNNPSIRKEFWAELSDLFGLTYPSWCVGGDFNVIRRRSEKLGGSRVTSSMR 271

Query: 530 RFNRFIDCAELQDIPLTNGKYTWSNFRPSPTMTLIDRFLISEHIPIKFGATTTRRLDRVT 589
            F+ FI  +EL D PL N  +TWSN + SP    +DRFL S    + F  +    L R T
Sbjct: 272 DFDGFIRESELHDSPLRNASFTWSNMQESPVCKRLDRFLYSNEWKLSFPQSLQEVLPRWT 331

Query: 590 SDHFPISLSLGNDRWGPTPFRFINVWLTHHSFLHTVDSWWKNTPFRGWPGHGFIQKLKGL 649
           SDH+PI L     +WGPTPFRF N+WL HH+F  +  SWW+     GW GH F++KL+ +
Sbjct: 332 SDHWPIVLDTNPFKWGPTPFRFENMWLQHHNFKESFSSWWREFEGNGWEGHKFMRKLQFV 391

Query: 650 KLELKQWNQNVFGNQKEQKTRLGQELLAIDNREENGHLSEQDPSRRTEIRAALITISAKE 709
           K +LK WN+N FG  KE+K  +  E+  ID  E+ G LS    + R   +  L  +  +E
Sbjct: 392 KAKLKDWNKNTFGLLKERKKSISDEIANIDAIEQEGALSSDLVALRAIRKGELEELILRE 451

Query: 710 EIMWRSKVQ 719
           EI W+ K +
Sbjct: 452 EIHWKQKAK 458

BLAST of Spg028694 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 3.0e-06
Identity = 47/166 (28.31%), Postives = 70/166 (42.17%), Query Frame = 0

Query: 504 ILGGDFN---VTRWSWEKSTFTAPSRAMRRFNRFIDCAELQDIPLTNGKYTWSNFR-PSP 563
           IL GDF+    T   +     + P R +  F   +  ++L DIP     YTWSN +  +P
Sbjct: 222 ILVGDFDQIAATSDHYSVLQTSIPMRGLEEFQNCLRDSDLVDIPSRGVHYTWSNHQDDNP 281

Query: 564 TMTLIDRFLISEHIPIKF-GATTTRRLDRVTSDHFPISLSLGN-DRWGPTPFRFINVWLT 623
            +  +DR + +      F  A     L  V SDH P  + L N  +     FR+ +   T
Sbjct: 282 IIRKLDRAIANGDWFSSFPSAIAVFELSGV-SDHSPCIIILENLPKRSKKCFRYFSFLST 341

Query: 624 HHSFLHTVDSWWKNTPFRGWPGHGFIQKLKGLKLELKQWNQNVFGN 664
           H +FL ++   W+     G       + LK  K   K  N+  FGN
Sbjct: 342 HPTFLVSLTVAWEEQIPVGSHMFSLGEHLKAAKKCCKLLNRQGFGN 386