Spg028673 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg028673 vs. NCBI nr
Match: XP_038892956.1 (subtilisin-like protease SBT4.14 [Benincasa hispida])

HSP 1 Score: 1286.9 bits (3329), Expect = 0.0e+00
Identity = 643/743 (86.54%), Postives = 692/743 (93.14%), Query Frame = 0

Query: 1   MSILKSSRLLVFVLFFVVAFIAAVEFDEEKKHFIVFLENKPVLNEVDAVETHLNVLMSVK 60
           MSI KSSRLLVF LFF+V  +AA++ D+EK HFIVFLENK VLNEVD VETHLNVLMSVK
Sbjct: 1   MSISKSSRLLVFALFFIVVCVAALDVDKEKNHFIVFLENKSVLNEVDGVETHLNVLMSVK 60

Query: 61  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWDFIG 120
           +SH EAKESMVYSY+KSFNAFAAKLTE+EAK LS REDVHHVIPNKYRKLQTTRSWDF+G
Sbjct: 61  KSHAEAKESMVYSYSKSFNAFAAKLTEDEAKKLSTREDVHHVIPNKYRKLQTTRSWDFLG 120

Query: 121 LPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 180
           L  NARRNTK ESDIVVGLFDTGITPTA+SFKDDGFGPPPKKWKG+CHH+ANFTGCNNKL
Sbjct: 121 LSSNARRNTKHESDIVVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHYANFTGCNNKL 180

Query: 181 IGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVPSAR 240
           IGARYFKLDGS DP DILSP+D DGHGTHTSSTATGNA+AGASLSGLA+GTARGGVPSAR
Sbjct: 181 IGARYFKLDGSPDPADILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVPSAR 240

Query: 241 VAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHAMKK 300
           V+MYKVCW S+GC+DMDILAAFDAAIHDGVDVISISIGGG F NYS+DSISIGAFHAMKK
Sbjct: 241 VSMYKVCWASSGCSDMDILAAFDAAIHDGVDVISISIGGGGFNNYSEDSISIGAFHAMKK 300

Query: 301 GIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTFNPK 360
           GIITVTSAGNSGP+AGS+VNHAPWIVTVAASAIDRKFISPLELGNGKNISGV IN FNPK
Sbjct: 301 GIITVTSAGNSGPLAGSVVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVGINIFNPK 360

Query: 361 VKMYPLVSGRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLGGSG 420
            KMYPLVSG DVARNSESK+SASYC E+SLD NKVKGNLV CK +TWG DSV+KSLG +G
Sbjct: 361 QKMYPLVSGGDVARNSESKDSASYCEEDSLDPNKVKGNLVFCKLMTWGADSVIKSLGANG 420

Query: 421 VIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPIVASF 480
           VIIQSDQFLDNADIFMAPAT+VSS +G  I  YIKST+TP AVIY+T+Q KA AP+VASF
Sbjct: 421 VIIQSDQFLDNADIFMAPATMVSSSLGTIIHTYIKSTRTPTAVIYKTKQLKAKAPMVASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVA 540
           SSRGPNPGSNRILKPDIAAPGVDILA YTPLKSLTGLKGDTQ+SKFTLMSGTSMACPHVA
Sbjct: 481 SSRGPNPGSNRILKPDIAAPGVDILASYTPLKSLTGLKGDTQYSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPHWSPAAVRSALITTARPISRRLNPDGEFAYGAGNLNPSQAKNPGLIYDL 600
           AAAAYVKSFHPHWSPAA+RSALITTARPISRRLNPDGEFAYGAGNLNPS+A +PGLIYD+
Sbjct: 541 AAAAYVKSFHPHWSPAAIRSALITTARPISRRLNPDGEFAYGAGNLNPSRAISPGLIYDV 600

Query: 601 NEMSYIQFLCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQRPMTT 660
           NE+SY+QFLC EGYT SSIA+LAGTKSINCS LIPG GHDSLNYPT QL+LK+T++P TT
Sbjct: 601 NEISYVQFLCSEGYTGSSIAVLAGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTRQPTTT 660

Query: 661 VFRRRVTNVGRAVSVYNATIRAPPGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKM 720
            FRRRVTNV   VSV+NATI+APPGVEITV PTTLSFSRLLQKRSFK+VVKASPLPSTKM
Sbjct: 661 TFRRRVTNVAHPVSVFNATIKAPPGVEITVTPTTLSFSRLLQKRSFKVVVKASPLPSTKM 720

Query: 721 VSGSLAWVGARHVVRSPIVVYSP 744
           VSGS+AW+GARHVVRSPIVVYSP
Sbjct: 721 VSGSIAWIGARHVVRSPIVVYSP 743

BLAST of Spg028673 vs. NCBI nr
Match: XP_011654432.1 (subtilisin-like protease SBT4.14 [Cucumis sativus] >KGN49552.1 hypothetical protein Csa_018364 [Cucumis sativus])

HSP 1 Score: 1250.0 bits (3233), Expect = 0.0e+00
Identity = 627/743 (84.39%), Postives = 680/743 (91.52%), Query Frame = 0

Query: 1   MSILKSSRLLVFVLFFVVAFIAAVEFDEEKKHFIVFLENKPVLNEVDAVETHLNVLMSVK 60
           MSI KSSRLLVF LF VV  +A ++ DEEK H+IVFLENKPVLNEVD VETHLN+LMSVK
Sbjct: 1   MSISKSSRLLVFALFIVVGCVAGLDEDEEKNHYIVFLENKPVLNEVDVVETHLNLLMSVK 60

Query: 61  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWDFIG 120
           +SH EA ESMVYSYTKSFNAFAAKL+++EAK LS R+DVHHVIPNKYRKLQTTRSWDFIG
Sbjct: 61  KSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIG 120

Query: 121 LPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 180
           L  NARR+TK ESDI+VGLFDTGITPTA+SFKDDGFGPPPKKWKG+CHHFANFT CN KL
Sbjct: 121 LSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKL 180

Query: 181 IGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVPSAR 240
           IGARYFKLDG+ DP+DILSP+D DGHGTHTSSTATGNAIAGASLSGLA+GTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSAR 240

Query: 241 VAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHAMKK 300
           VAMYKVCW S+GC+DMDILAAFDAAI DGVDVISISIGGG F NYSDDSISIGAFHAMKK
Sbjct: 241 VAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKK 300

Query: 301 GIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTFNPK 360
           GIITVTSAGN GP AGS+VNHAPWIVTVAAS+IDRKFISPLELGNGKNISGV IN FNPK
Sbjct: 301 GIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPK 360

Query: 361 VKMYPLVSGRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLGGSG 420
            KMYPLVSG DVARNSESK++AS+C E +LD  KVKG+LV CK LTWG DSV+KS+G +G
Sbjct: 361 QKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANG 420

Query: 421 VIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPIVASF 480
           VIIQSD+FLDNADIFMAPAT+VSSLVG+ I  YIKST+TP AVIY+T+Q KA AP+VASF
Sbjct: 421 VIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMVASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVA 540
           SSRGPNPGS+RILKPDIAAPGVDILA YTPLKSLTG KGDTQ+SKFTLMSGTSMACPHVA
Sbjct: 481 SSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPHWSPAAVRSALITTARPISRRLNPDGEFAYGAGNLNPSQAKNPGLIYDL 600
           AAAAYVKSFHP WSPAA+RSAL+TTA PISRRLNP+GEFAYGAGNLNPS+A +PGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLIYDL 600

Query: 601 NEMSYIQFLCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQRPMTT 660
           NE+SYIQFLC EGYT SSIA+L+GTKSINCS LIPG GHDSLNYPT QL+LK+T +PMTT
Sbjct: 601 NEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTT 660

Query: 661 VFRRRVTNVGRAVSVYNATIRAPPGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKM 720
            FRRRVTNVG  +SVYNATI APPGV ITV P TLSFSRLLQKRSFK+VVKASPLPS KM
Sbjct: 661 TFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSAKM 720

Query: 721 VSGSLAWVGARHVVRSPIVVYSP 744
           VSGSLAWVGA+HVVRSPIVVYSP
Sbjct: 721 VSGSLAWVGAQHVVRSPIVVYSP 743

BLAST of Spg028673 vs. NCBI nr
Match: XP_008454753.1 (PREDICTED: subtilisin-like protease SBT4.14 [Cucumis melo] >KAA0056482.1 subtilisin-like protease SBT4.14 [Cucumis melo var. makuwa])

HSP 1 Score: 1238.8 bits (3204), Expect = 0.0e+00
Identity = 624/746 (83.65%), Postives = 681/746 (91.29%), Query Frame = 0

Query: 1   MSILKSSRLLVFVLFFVVAFIAA---VEFDEEKKHFIVFLENKPVLNEVDAVETHLNVLM 60
           MS+ KSSRLLVF LF +V  +      + +EEK HFIVFL+NKPVLNEVDAVETHL+VLM
Sbjct: 1   MSLSKSSRLLVFALFIIVGCVVQGMDRDENEEKNHFIVFLQNKPVLNEVDAVETHLSVLM 60

Query: 61  SVKESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWD 120
           SVK+SH EA ESMVYSYTKSFNAFAAKL++ EAK LS R+DVHHVIPN YRKLQTTRSWD
Sbjct: 61  SVKKSHAEASESMVYSYTKSFNAFAAKLSDHEAKLLSTRKDVHHVIPNTYRKLQTTRSWD 120

Query: 121 FIGLPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCN 180
           FIGL  NARR TK ESDI+VGLFDTGITPTA+SF+DDGFGPPPKKWKG+CHHFANFT CN
Sbjct: 121 FIGLSSNARRRTKHESDIIVGLFDTGITPTADSFRDDGFGPPPKKWKGTCHHFANFTACN 180

Query: 181 NKLIGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVP 240
            KLIGARYFKLDG+ DP+DILSP+D DGHGTHTSSTATGNA+AGASLSGLA+GTARGGVP
Sbjct: 181 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVP 240

Query: 241 SARVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHA 300
           SARVAMYKVCW S+GC+DMDILAAFDAAI DGVDVISISIGGG F NYSDDSISIGAFHA
Sbjct: 241 SARVAMYKVCWASSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 300

Query: 301 MKKGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTF 360
           MKKGIITVTSAGN GP+AGS+VNHAPWIVTVAAS+IDRKFISPLELGNGKNISGV IN F
Sbjct: 301 MKKGIITVTSAGNGGPMAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 360

Query: 361 NPKVKMYPLVSGRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLG 420
           NPK KMYPLVSG DVARNSESK++AS+C E+SLD  KVKG+LV CK LTWG DSV+KS+G
Sbjct: 361 NPKKKMYPLVSGGDVARNSESKDTASFCLEDSLDPTKVKGSLVFCKLLTWGADSVIKSVG 420

Query: 421 GSGVIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPIV 480
            +GVIIQSDQFLDNADIFMAPAT+VSS VG  I  YIKST+TP AVIY+TRQ KA AP+V
Sbjct: 421 ANGVIIQSDQFLDNADIFMAPATMVSSSVGTIIYTYIKSTRTPTAVIYKTRQLKAKAPMV 480

Query: 481 ASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACP 540
           ASFSSRGPNPGS+RILKPDIAAPGV+ILAGYTPLKSLTG KGDTQ+SKFT+MSGTSMACP
Sbjct: 481 ASFSSRGPNPGSHRILKPDIAAPGVNILAGYTPLKSLTGQKGDTQYSKFTIMSGTSMACP 540

Query: 541 HVAAAAAYVKSFHPHWSPAAVRSALITTARPISRRLNPDGEFAYGAGNLNPSQAKNPGLI 600
           HVAAAAAYVKSFHP WSPAA+RSAL+TT RPISRRLNP+GEFAYGAGNLNPS+A +PGLI
Sbjct: 541 HVAAAAAYVKSFHPLWSPAAIRSALLTTTRPISRRLNPEGEFAYGAGNLNPSKAISPGLI 600

Query: 601 YDLNEMSYIQFLCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQRP 660
           YDLNEMSYIQFLC EGYT SSIA+LAGTKSINCSTLIPG GHDSLNYPT QL+L++T++P
Sbjct: 601 YDLNEMSYIQFLCSEGYTGSSIAVLAGTKSINCSTLIPGHGHDSLNYPTFQLSLRSTKQP 660

Query: 661 MTTVFRRRVTNVGRAVSVYNATIRAPPGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPS 720
           MTT FRR+VTNVG+ VSVYNATI+APPGV+ITV PTTLSFSRLLQKRSFK+VVKASPL S
Sbjct: 661 MTTTFRRQVTNVGQPVSVYNATIKAPPGVKITVTPTTLSFSRLLQKRSFKVVVKASPLQS 720

Query: 721 TKMVSGSLAWVGARHVVRSPIVVYSP 744
            KMVSGSLAWVG RHVVRSPIVVYSP
Sbjct: 721 AKMVSGSLAWVGDRHVVRSPIVVYSP 746

BLAST of Spg028673 vs. NCBI nr
Match: XP_023550181.1 (subtilisin-like protease SBT4.14 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1228.8 bits (3178), Expect = 0.0e+00
Identity = 625/743 (84.12%), Postives = 672/743 (90.44%), Query Frame = 0

Query: 1   MSILKSSRLLVFVLFFVVAFIAAVEFDEEKKHFIVFLENKPVLNEVDAVETHLNVLMSVK 60
           M I  SSR+LV VL FVV  + AVE DEEKKHFIVFLE KP LNE DAVETHLNVLMSVK
Sbjct: 1   MLISISSRVLVCVLLFVVGCVGAVEIDEEKKHFIVFLEMKPDLNEFDAVETHLNVLMSVK 60

Query: 61  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWDFIG 120
           ES+VEA+ESMVYSYTKSFNAFAAKL EEEA  LSKREDVHHVIPN+YRKLQTTRSWDFIG
Sbjct: 61  ESYVEAEESMVYSYTKSFNAFAAKLNEEEALALSKREDVHHVIPNQYRKLQTTRSWDFIG 120

Query: 121 LPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 180
           LP  ARRN+K E+DI+VGLFDTGITPTA+SFKDDGFGPPPKKWKG+CHHFANFTGCN KL
Sbjct: 121 LPSKARRNSK-ETDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTGCNRKL 180

Query: 181 IGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVPSAR 240
           IGARYFKLDG+ DP DILSPIDVDGHGTHTSSTATGNA+AGASLSGLAKGTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSAR 240

Query: 241 VAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHAMKK 300
           VAMYKVCW STGCADMDILAAFDAAIHDGVDVISISIGGG+FGNYSDDSISIGAFHA+KK
Sbjct: 241 VAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHALKK 300

Query: 301 GIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTFNPK 360
           GIITVTSAGN GP  GS+VNHAPWIVTV ASAIDRKFIS LELGNGKNISGV IN FNPK
Sbjct: 301 GIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPK 360

Query: 361 VKMYPLVSGRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLGGSG 420
            KMYPLV G DVAR +E++ESAS+CAE+SLD +KVKG+LV C+ +TWG DSVV +LG +G
Sbjct: 361 KKMYPLVYGGDVARGAENRESASHCAEDSLDPSKVKGSLVFCELITWGVDSVVNALGANG 420

Query: 421 VIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPIVASF 480
            IIQSD++LDNA+IFMAPAT+VSS VG  I +YIKST+TP AVIY+TRQ KA AP+ ASF
Sbjct: 421 AIIQSDEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVA 540
           SSRGPNPG+ RILKPDIAAPGVDILAGYTPLKSLTG +GDTQFSKFTLMSGTSMACPHVA
Sbjct: 481 SSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPHWSPAAVRSALITTARPISRRLNPDGEFAYGAGNLNPSQAKNPGLIYDL 600
           AAAAYVKSFHP WSPAA+RSALITTA  ISRRLNPDGEFAYGAGNLNPS+A NPGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPAAIRSALITTASRISRRLNPDGEFAYGAGNLNPSRAINPGLIYDL 600

Query: 601 NEMSYIQFLCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQRPMTT 660
           NEMSYIQFLCREGYT SSI +LAGTKSINCSTLIPG GHDSLNYPT QL LK+ +R M+T
Sbjct: 601 NEMSYIQFLCREGYTGSSIGVLAGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMST 660

Query: 661 VFRRRVTNVGRAVSVYNATIRAPPGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKM 720
           VFRRRVTNVG  VSVYNATI+APPGVEITV P+TLSF RL QKRSFK+ VKA PL S KM
Sbjct: 661 VFRRRVTNVGHPVSVYNATIKAPPGVEITVTPSTLSFLRLQQKRSFKVAVKAGPLASGKM 720

Query: 721 VSGSLAWVGARHVVRSPIVVYSP 744
           VSGS+AW+GARH VRSPIVVYSP
Sbjct: 721 VSGSVAWIGARHAVRSPIVVYSP 742

BLAST of Spg028673 vs. NCBI nr
Match: XP_022941706.1 (subtilisin-like protease SBT4.14 [Cucurbita moschata])

HSP 1 Score: 1225.7 bits (3170), Expect = 0.0e+00
Identity = 622/743 (83.71%), Postives = 672/743 (90.44%), Query Frame = 0

Query: 1   MSILKSSRLLVFVLFFVVAFIAAVEFDEEKKHFIVFLENKPVLNEVDAVETHLNVLMSVK 60
           M I  SSR+LV VL FV+  + AVE DEEKKHFIVFLE KP LNE DAVETHLNVLMSVK
Sbjct: 1   MLISISSRVLVCVLLFVIGCVGAVEIDEEKKHFIVFLEMKPDLNEFDAVETHLNVLMSVK 60

Query: 61  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWDFIG 120
           ES+VEA+ESMVYSYTKSFNAFAAKLTE+EA  LSKREDVHHVIPN+YRKLQTTRSWDFI 
Sbjct: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEDEALALSKREDVHHVIPNRYRKLQTTRSWDFIE 120

Query: 121 LPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 180
           LP  ARRN+K E+DI+VGLFDTGITPTA+SFKDDGFGPPPKKWKG+CHHFANFTGCN KL
Sbjct: 121 LPSKARRNSK-ETDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTGCNQKL 180

Query: 181 IGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVPSAR 240
           IGARYFKLDG+ DP DILSPIDVDGHGTHTSSTATGNA+AGASLSGLAKGTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSAR 240

Query: 241 VAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHAMKK 300
           VAMYKVCW STGCADMDILAAFDAAIHDGVDVISISIGGG+FGNYSDDSISIGAFHA+KK
Sbjct: 241 VAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHALKK 300

Query: 301 GIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTFNPK 360
           GIITVTSAGN GP  GS+VNHAPWIVTV ASAIDRKFIS LELGNGKNISGV INTFNPK
Sbjct: 301 GIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINTFNPK 360

Query: 361 VKMYPLVSGRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLGGSG 420
            KMYPLV G DVAR +E++ESAS+CAE+SLD +KVKG+LV C+ +TWG DSVV +LG +G
Sbjct: 361 KKMYPLVYGGDVARGAENRESASHCAEDSLDPSKVKGSLVFCELITWGVDSVVNALGANG 420

Query: 421 VIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPIVASF 480
            IIQSD++LDNA+IFMAPAT+VSS VG  I +YIKST+TP AVIY+TRQ KA AP+ ASF
Sbjct: 421 AIIQSDEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVA 540
           SSRGPNPG+ RILKPDIAAPGVDILAGYTPLKSLTG +GDTQFSKFTLMSGTSMACPHVA
Sbjct: 481 SSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPHWSPAAVRSALITTARPISRRLNPDGEFAYGAGNLNPSQAKNPGLIYDL 600
           AAAAYVKSFHP WSP A+RSALITTA  ISR+LNPDGEFAYGAGNLNPS+A NPGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPGAIRSALITTASRISRQLNPDGEFAYGAGNLNPSRAINPGLIYDL 600

Query: 601 NEMSYIQFLCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQRPMTT 660
           NEMSYIQFLC EGYT SSI +LAGTKSINCSTLIPG GHDSLNYPT QL LK+ QR M+T
Sbjct: 601 NEMSYIQFLCSEGYTGSSIGVLAGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPQRAMST 660

Query: 661 VFRRRVTNVGRAVSVYNATIRAPPGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKM 720
           VFRRRVTNVG  VSVYNATI+APPGVEITV P+TLSFS+L QKRSFK+ VKA PL S KM
Sbjct: 661 VFRRRVTNVGHPVSVYNATIKAPPGVEITVTPSTLSFSQLQQKRSFKVAVKAGPLASGKM 720

Query: 721 VSGSLAWVGARHVVRSPIVVYSP 744
           VSGS+AW+GARH VRSPIVVYSP
Sbjct: 721 VSGSVAWIGARHAVRSPIVVYSP 742

BLAST of Spg028673 vs. ExPASy Swiss-Prot
Match: Q9LLL8 (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 1.8e-264
Identity = 452/735 (61.50%), Postives = 571/735 (77.69%), Query Frame = 0

Query: 9   LLVFVLFFVVAFIAAVEFDEEKKHFIVFLENKPVLNEVDAVETHLNVLMSVKESHVEAKE 68
           +LV V+ ++    A+ E +  K  +I++L ++P  N  + ++TH+N+L S+  S  EAKE
Sbjct: 14  VLVMVVLWISPRYASAEDEHAKDFYIIYLGDRPD-NTEETIKTHINLLSSLNISQEEAKE 73

Query: 69  SMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWDFIGLPINARRN 128
             VYSYTK+FNAFAAKL+  EAK + + E+V  V  N+YRKL TT+SWDF+GLP+ A+R+
Sbjct: 74  RKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLTAKRH 133

Query: 129 TKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKLIGARYFKL 188
            K E D+++G+ DTGITP +ESF D G GPPP KWKGSC  + NFTGCNNK+IGA+YFK 
Sbjct: 134 LKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKH 193

Query: 189 DGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVPSARVAMYKVCW 248
           DG+    ++ SPID+DGHGTHTSST  G  +A ASL G+A GTARG VPSAR+AMYKVCW
Sbjct: 194 DGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCW 253

Query: 249 ESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHAMKKGIITVTSA 308
             +GCADMDILA F+AAIHDGV++ISISI GG   +YS DSIS+G+FHAM+KGI+TV SA
Sbjct: 254 ARSGCADMDILAGFEAAIHDGVEIISISI-GGPIADYSSDSISVGSFHAMRKGILTVASA 313

Query: 309 GNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTFNPKVKMYPLVS 368
           GN GP +G++ NH PWI+TVAAS IDR F S ++LGNGK+ SG+ I+ F+PK K YPLVS
Sbjct: 314 GNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVS 373

Query: 369 GRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLGGSGVIIQSDQF 428
           G D A+N++ K  A YC  +SLDR KVKG +++C+    G +S +KS GG+G II SDQ+
Sbjct: 374 GVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQY 433

Query: 429 LDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPIVASFSSRGPNPG 488
           LDNA IFMAPAT V+S VGD I  YI ST++  AVI +TRQ    AP VASFSSRGPNPG
Sbjct: 434 LDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASFSSRGPNPG 493

Query: 489 SNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKS 548
           S R+LKPDIAAPG+DILA +T  +SLTGL GDTQFSKFT++SGTSMACPHVA  AAYVKS
Sbjct: 494 SIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVKS 553

Query: 549 FHPHWSPAAVRSALITTARPISRRLNPDGEFAYGAGNLNPSQAKNPGLIYDLNEMSYIQF 608
           FHP W+PAA++SA+IT+A+PISRR+N D EFAYG G +NP +A +PGL+YD++++SY+QF
Sbjct: 554 FHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDISYVQF 613

Query: 609 LCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQRPMTTVFRRRVTN 668
           LC EGY  +++A L GT+S++CS+++PGLGHDSLNYPTIQL L++ +     VFRRRVTN
Sbjct: 614 LCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFRRRVTN 673

Query: 669 VGRAVSVYNATIRAPPGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKMVSGSLAWV 728
           VG   SVY AT+RAP GVEITV P +LSFS+  QKRSFK+VVKA  +   K+VSG L W 
Sbjct: 674 VGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKIVSGLLVWK 733

Query: 729 GARHVVRSPIVVYSP 744
             RH VRSPIV+YSP
Sbjct: 734 SPRHSVRSPIVIYSP 746

BLAST of Spg028673 vs. ExPASy Swiss-Prot
Match: Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 8.5e-182
Identity = 366/767 (47.72%), Postives = 501/767 (65.32%), Query Frame = 0

Query: 3   ILKSSRLLVFVLFFVV---AFIAAVEFDE-EKKHFIVFLENKPVLNEVDAVETHLNVLMS 62
           ++ + R+ +F+L F +   A IAA E +  E+K +IV++      + V+A E H N+LM+
Sbjct: 1   MVSNQRVRLFMLCFCLVNNAVIAATEDENVERKPYIVYMGEATENSLVEAAENHHNLLMT 60

Query: 63  VKESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWDF 122
           V     +A+E  +YSY K+ N F A+L   EA+ LS+ E V  V  N  R+L TTRSWDF
Sbjct: 61  VIGDESKARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDF 120

Query: 123 IGL-PINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCN 182
           +GL     +R+   ES+I+VG+ DTGI   + SF D G GPPP KWKG C    NFT CN
Sbjct: 121 LGLVESKYKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCN 180

Query: 183 NKLIGARYFKL------DGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGT 242
           NK+IGA+YF +      DG  D     +  D DGHGTHTSST  G +++ ASL G+A GT
Sbjct: 181 NKVIGAKYFHIQSEGLPDGEGD-----TAADHDGHGTHTSSTIAGVSVSSASLFGIANGT 240

Query: 243 ARGGVPSARVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSIS 302
           ARGGVPSAR+A YKVCW+S GC DMD+LAAFD AI DGVD+ISISIGG S   + +D I+
Sbjct: 241 ARGGVPSARIAAYKVCWDS-GCTDMDMLAAFDEAISDGVDIISISIGGASL-PFFEDPIA 300

Query: 303 IGAFHAMKKGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISG 362
           IGAFHAMK+GI+T  SAGN+GP   ++ N APW++TVAA+++DRKF + ++LGNG   SG
Sbjct: 301 IGAFHAMKRGILTTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASG 360

Query: 363 VAINTFNPKVKMYPLVSGRDVAR-NSESKESASYCAEESLDRNKVKGNLVLCKFLT---- 422
           +++N FNP+ KMYPL SG   +  ++      S C   +L  +KV G +V C+       
Sbjct: 361 ISLNGFNPRKKMYPLTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGG 420

Query: 423 ---WGTDSVVKSLGGSGVIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAV 482
               G D VV+SL G+GVI+Q  +  D A   +   + V    G  I  YI STK P+AV
Sbjct: 421 NGGQGQDHVVRSLKGAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAV 480

Query: 483 IYRTRQFKATAPIVASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQF 542
           I++T+  K  AP ++SFS+RGP   S  ILKPDI+APG++ILA Y+ L S+TG   D + 
Sbjct: 481 IFKTKTTKMLAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRR 540

Query: 543 SKFTLMSGTSMACPHVAAAAAYVKSFHPHWSPAAVRSALITTARPISRRLNPDGEFAYGA 602
           + F++MSGTSMACPH AAAAAYVKSFHP WSPAA++SAL+TTA P+  + N + E +YG+
Sbjct: 541 TLFSIMSGTSMACPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGS 600

Query: 603 GNLNPSQAKNPGLIYDLNEMSYIQFLCREGYTDSSIAILAG-------TKSINCSTLIPG 662
           G +NP +A +PGL+YD+ E +Y++FLC+EGY  +SI +L G        K  NC  +  G
Sbjct: 601 GQINPRRAIHPGLVYDITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRG 660

Query: 663 LGHDSLNYPTIQLNLKNTQRPMTTVFRRRVTNVGRAVSVYNATIRAPPGVEITVVPTTLS 722
           LG D LNYP++   + +T+  ++ VF R VTNVG   S Y A + AP G+ + VVP  +S
Sbjct: 661 LGSDGLNYPSLHKQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMS 720

Query: 723 FSRLLQKRSFKIVVKASPLPSTK-MVSGSLAWVGAR-HVVRSPIVVY 742
           F R  +KR+FK+V+      + K +VS S+ W  +R H+VRSPI+++
Sbjct: 721 FERPKEKRNFKVVIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLF 759

BLAST of Spg028673 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 6.5e-166
Identity = 322/739 (43.57%), Postives = 478/739 (64.68%), Query Frame = 0

Query: 8   RLLVFVLFFVVAFIAAVEFDEEKKH-FIVFLENKPVLNEVDAVETHLNVLMSVKESHVEA 67
           +L  F LFF     + ++ D++ K+ +IV++  K  L + D+   H   ++        A
Sbjct: 8   KLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRK--LEDPDSAHLHHRAMLEQVVGSTFA 67

Query: 68  KESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWDFIGLPINAR 127
            ES++++Y +SFN FA KLTEEEA+ ++  E V  V  N+  +L TTRSWDF+G P+   
Sbjct: 68  PESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLTVP 127

Query: 128 RNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKLIGARYF 187
           R ++ ES+IVVG+ DTGI P + SF D+GF PPP KWKG+C    NF  CN K+IGAR +
Sbjct: 128 RRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFR-CNRKIIGARSY 187

Query: 188 KLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVPSARVAMYKV 247
            +     P D+  P D +GHGTHT+STA G  ++ A+L GL  GTARGGVP AR+A YKV
Sbjct: 188 HIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKV 247

Query: 248 CWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHAMKKGIITVT 307
           CW + GC+D DILAA+D AI DGVD+IS+S+GG +  +Y  D+I+IG+FHA+++GI+T  
Sbjct: 248 CW-NDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSN 307

Query: 308 SAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTFNPKVKMYPL 367
           SAGN GP   +  + +PW+++VAAS +DRKF++ +++GNG++  GV+INTF+   + YPL
Sbjct: 308 SAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDN--QYYPL 367

Query: 368 VSGRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLGGSGVIIQSD 427
           VSGRD+      K ++ +C ++S++ N +KG +V+C+  ++G     KSL G+  ++ + 
Sbjct: 368 VSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCE-ASFGPHEFFKSLDGAAGVLMTS 427

Query: 428 QFLDNADIFMAPATVVSSLVGDTIA--NYIKSTKTPKAVIYR-TRQFKATAPIVASFSSR 487
              D AD +  P++V+     D +A   YI S ++P A I++ T    A+AP+V SFSSR
Sbjct: 428 NTRDYADSYPLPSSVLDP--NDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 487

Query: 488 GPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAA 547
           GPN  +  ++KPDI+ PGV+ILA +  +  + G++ +T F+   ++SGTSM+CPH+   A
Sbjct: 488 GPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFN---IISGTSMSCPHITGIA 547

Query: 548 AYVKSFHPHWSPAAVRSALITTARPISRRLNPDGEFAYGAGNLNPSQAKNPGLIYDLNEM 607
            YVK+++P WSPAA++SAL+TTA P++ R NP  EFAYG+G++NP +A  PGL+YD NE 
Sbjct: 548 TYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANES 607

Query: 608 SYIQFLCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQRPMTTVFR 667
            Y++FLC +GY   ++  + G  S  C++   G   D LNYP+  L++  +Q      F 
Sbjct: 608 DYVKFLCGQGYNTQAVRRITGDYSA-CTSGNTGRVWD-LNYPSFGLSVSPSQ-TFNQYFN 667

Query: 668 RRVTNVGRAVSVYNATIRAPPGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKMVSG 727
           R +T+V    S Y A I AP G+ I+V P  LSF+ L  ++SF + V+ S      +VS 
Sbjct: 668 RTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGS--IKGFVVSA 727

Query: 728 SLAWVGARHVVRSPIVVYS 743
           SL W    H VRSPI + S
Sbjct: 728 SLVWSDGVHYVRSPITITS 729

BLAST of Spg028673 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 2.0e-162
Identity = 325/746 (43.57%), Postives = 470/746 (63.00%), Query Frame = 0

Query: 1   MSILKSSRLLVFVLFFVVAFIAAVEFDEEKKHFIVFLENKPVLNEVDAVETHLNVLMSVK 60
           M+ L +   L+ + F     ++A ++ +    +IV++   P + +      HL++L  + 
Sbjct: 1   MAKLSTPLYLICLAFIFTRDVSANDYRQASSVYIVYMGTLPEI-KYSPPSHHLSILQKLV 60

Query: 61  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWDFIG 120
            + + A   +V SY +SFN FAA L++ E++ L   ++V  V P+K  +L TTRSWDF+G
Sbjct: 61  GT-IAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVG 120

Query: 121 LPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 180
               ARR + +ESD++VG+ D+GI P +ESF D+GFGPPPKKWKGSC     F  CNNKL
Sbjct: 121 FGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKF-ACNNKL 180

Query: 181 IGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVPSAR 240
           IGAR++            S  D +GHGTHT+STA GNA+  AS  GLA+GTARGGVPSAR
Sbjct: 181 IGARFYNKFAD-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSAR 240

Query: 241 VAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHAMKK 300
           +A YKVC+    C D+DILAAFD AI DGVDVISISI      N  + S++IG+FHAM +
Sbjct: 241 IAAYKVCFNR--CNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMR 300

Query: 301 GIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTFNPK 360
           GIIT  SAGN+GP  GS+ N +PW++TVAAS  DR+FI  + LGNGK ++G+++NTFN  
Sbjct: 301 GIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLN 360

Query: 361 VKMYPLVSGRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLGGSG 420
              +P+V G++V+RN  S+  A YC+   +D   VKG +VLC       ++ +   G  G
Sbjct: 361 GTKFPIVYGQNVSRNC-SQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYL--AGAIG 420

Query: 421 VIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQF-KATAPIVAS 480
           VI+Q+    D+A +   PA+ +      +I +YI+S + P+A I RT +     AP V S
Sbjct: 421 VIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPS 480

Query: 481 FSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKG--DTQFSKFTLMSGTSMACP 540
           FSSRGP+     +LKPD++APG++ILA ++P+ S +      D +  ++++MSGTSMACP
Sbjct: 481 FSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACP 540

Query: 541 HVAAAAAYVKSFHPHWSPAAVRSALITTARPISRRLNPDGEFAYGAGNLNPSQAKNPGLI 600
           HVA  AAYVKSFHP WSP+A++SA++TTA P++ + NP+ EFAYG+G +NP++A +PGL+
Sbjct: 541 HVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLV 600

Query: 601 YDLNEMSYIQFLCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQRP 660
           Y++    Y++ LC EG+  +++   +G +++ CS          LNYPT+   + +   P
Sbjct: 601 YEVETEDYLKMLCAEGFDSTTLTTTSG-QNVTCSERTE---VKDLNYPTMTTFVSSLD-P 660

Query: 661 MTTVFRRRVTNVGRAVSVYNAT-IRAPPGVEITVVPTTLSFSRLLQKRSFKIVVKASPLP 720
               F+R VTNVG   S Y A+ +   P ++I++ P  L F  L +K+SF + +    L 
Sbjct: 661 FNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELK 720

Query: 721 STKMVSGSLAWVGARHVVRSPIVVYS 743
               VS S+ W    H VRSPIV YS
Sbjct: 721 DGSFVSSSVVWSDGSHSVRSPIVAYS 726

BLAST of Spg028673 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 7.7e-159
Identity = 326/749 (43.52%), Postives = 478/749 (63.82%), Query Frame = 0

Query: 2   SILKSSRLLVFVLFFVVAFIAAVEFDEEKKHFIVFLENKPVLNEVDAVETHLNVLMSVK- 61
           ++  SS LL  +L   ++ ++AV   ++K+ +IV++ +     +      H+N+L  V  
Sbjct: 3   TLAASSSLLSCLLVLFLSSVSAV--TDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTG 62

Query: 62  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWDFIG 121
           ES +E +  +V SY +SFN FAA+LTE E + ++K   V  V PNK  +LQTT SWDF+G
Sbjct: 63  ESSIEGR--LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMG 122

Query: 122 LP--INARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNN 181
           L   I  +RN   ESD ++G+ D+GITP ++SF D GFGPPP+KWKG C    NFT CNN
Sbjct: 123 LKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFT-CNN 182

Query: 182 KLIGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVPS 241
           KLIGAR +  +G+          D+DGHGTHT+STA GNA+  AS  G+  GT RGGVP+
Sbjct: 183 KLIGARDYTSEGTR---------DMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPA 242

Query: 242 ARVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHAM 301
           +RVA YKVC   TGC+   +L+AFD AI DGVD+I+ISIG  +   + +D I+IGAFHAM
Sbjct: 243 SRVAAYKVC-TPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAM 302

Query: 302 KKGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTFN 361
            KG++TV SAGNSGP   S+   APWI+TVAAS  +R F++ + LGNGK + G ++N + 
Sbjct: 303 AKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYE 362

Query: 362 PKVKMYPLVSGRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLGG 421
            K K YPLV G+  A ++   ESA  C    +D+++VKG +++C     G   +V+S+G 
Sbjct: 363 MKGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCG--GPGGLKIVESVGA 422

Query: 422 SGVIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQ-FKATAPIV 481
            G+I ++ +  D A I   PA  + +   +++ +Y++ST +P+A++ +T   F  T+P++
Sbjct: 423 VGLIYRTPK-PDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVI 482

Query: 482 ASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACP 541
           ASFSSRGPN  +  ILKPDI APGV+ILA Y+P    +  + DT+  K++++SGTSM+CP
Sbjct: 483 ASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCP 542

Query: 542 HVAAAAAYVKSFHPHWSPAAVRSALITTARPISRRLN--PDGEFAYGAGNLNPSQAKNPG 601
           HVA  AAYVK+F+P WSP+ ++SA++TTA P++         EFAYG+G+++P  A NPG
Sbjct: 543 HVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPG 602

Query: 602 LIYDLNEMSYIQFLCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQ 661
           L+Y+L++  +I FLC   YT   + +++G +++ CS     L   +LNYP++   L  + 
Sbjct: 603 LVYELDKSDHIAFLCGMNYTSQVLKVISG-ETVTCSEAKKILPR-NLNYPSMSAKLSGSG 662

Query: 662 RPMTTVFRRRVTNVGRAVSVYNATIRAPPG--VEITVVPTTLSFSRLLQKRSFKIVVKAS 721
              T  F R +TNVG   S Y + + A  G  +++ + P+ LSF  + +K+SF + V  S
Sbjct: 663 TTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGS 722

Query: 722 PLPSTKMVSGSLAWVGARHVVRSPIVVYS 743
            L S    S +L W    H VRSPIVVY+
Sbjct: 723 NLDSEVPSSANLIWSDGTHNVRSPIVVYT 729

BLAST of Spg028673 vs. ExPASy TrEMBL
Match: A0A0A0KME6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G000010 PE=3 SV=1)

HSP 1 Score: 1250.0 bits (3233), Expect = 0.0e+00
Identity = 627/743 (84.39%), Postives = 680/743 (91.52%), Query Frame = 0

Query: 1   MSILKSSRLLVFVLFFVVAFIAAVEFDEEKKHFIVFLENKPVLNEVDAVETHLNVLMSVK 60
           MSI KSSRLLVF LF VV  +A ++ DEEK H+IVFLENKPVLNEVD VETHLN+LMSVK
Sbjct: 1   MSISKSSRLLVFALFIVVGCVAGLDEDEEKNHYIVFLENKPVLNEVDVVETHLNLLMSVK 60

Query: 61  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWDFIG 120
           +SH EA ESMVYSYTKSFNAFAAKL+++EAK LS R+DVHHVIPNKYRKLQTTRSWDFIG
Sbjct: 61  KSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIG 120

Query: 121 LPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 180
           L  NARR+TK ESDI+VGLFDTGITPTA+SFKDDGFGPPPKKWKG+CHHFANFT CN KL
Sbjct: 121 LSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKL 180

Query: 181 IGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVPSAR 240
           IGARYFKLDG+ DP+DILSP+D DGHGTHTSSTATGNAIAGASLSGLA+GTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSAR 240

Query: 241 VAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHAMKK 300
           VAMYKVCW S+GC+DMDILAAFDAAI DGVDVISISIGGG F NYSDDSISIGAFHAMKK
Sbjct: 241 VAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKK 300

Query: 301 GIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTFNPK 360
           GIITVTSAGN GP AGS+VNHAPWIVTVAAS+IDRKFISPLELGNGKNISGV IN FNPK
Sbjct: 301 GIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPK 360

Query: 361 VKMYPLVSGRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLGGSG 420
            KMYPLVSG DVARNSESK++AS+C E +LD  KVKG+LV CK LTWG DSV+KS+G +G
Sbjct: 361 QKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANG 420

Query: 421 VIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPIVASF 480
           VIIQSD+FLDNADIFMAPAT+VSSLVG+ I  YIKST+TP AVIY+T+Q KA AP+VASF
Sbjct: 421 VIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMVASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVA 540
           SSRGPNPGS+RILKPDIAAPGVDILA YTPLKSLTG KGDTQ+SKFTLMSGTSMACPHVA
Sbjct: 481 SSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPHWSPAAVRSALITTARPISRRLNPDGEFAYGAGNLNPSQAKNPGLIYDL 600
           AAAAYVKSFHP WSPAA+RSAL+TTA PISRRLNP+GEFAYGAGNLNPS+A +PGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLIYDL 600

Query: 601 NEMSYIQFLCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQRPMTT 660
           NE+SYIQFLC EGYT SSIA+L+GTKSINCS LIPG GHDSLNYPT QL+LK+T +PMTT
Sbjct: 601 NEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTT 660

Query: 661 VFRRRVTNVGRAVSVYNATIRAPPGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKM 720
            FRRRVTNVG  +SVYNATI APPGV ITV P TLSFSRLLQKRSFK+VVKASPLPS KM
Sbjct: 661 TFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSAKM 720

Query: 721 VSGSLAWVGARHVVRSPIVVYSP 744
           VSGSLAWVGA+HVVRSPIVVYSP
Sbjct: 721 VSGSLAWVGAQHVVRSPIVVYSP 743

BLAST of Spg028673 vs. ExPASy TrEMBL
Match: A0A5A7UKY8 (Subtilisin-like protease SBT4.14 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold186G002590 PE=3 SV=1)

HSP 1 Score: 1238.8 bits (3204), Expect = 0.0e+00
Identity = 624/746 (83.65%), Postives = 681/746 (91.29%), Query Frame = 0

Query: 1   MSILKSSRLLVFVLFFVVAFIAA---VEFDEEKKHFIVFLENKPVLNEVDAVETHLNVLM 60
           MS+ KSSRLLVF LF +V  +      + +EEK HFIVFL+NKPVLNEVDAVETHL+VLM
Sbjct: 1   MSLSKSSRLLVFALFIIVGCVVQGMDRDENEEKNHFIVFLQNKPVLNEVDAVETHLSVLM 60

Query: 61  SVKESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWD 120
           SVK+SH EA ESMVYSYTKSFNAFAAKL++ EAK LS R+DVHHVIPN YRKLQTTRSWD
Sbjct: 61  SVKKSHAEASESMVYSYTKSFNAFAAKLSDHEAKLLSTRKDVHHVIPNTYRKLQTTRSWD 120

Query: 121 FIGLPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCN 180
           FIGL  NARR TK ESDI+VGLFDTGITPTA+SF+DDGFGPPPKKWKG+CHHFANFT CN
Sbjct: 121 FIGLSSNARRRTKHESDIIVGLFDTGITPTADSFRDDGFGPPPKKWKGTCHHFANFTACN 180

Query: 181 NKLIGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVP 240
            KLIGARYFKLDG+ DP+DILSP+D DGHGTHTSSTATGNA+AGASLSGLA+GTARGGVP
Sbjct: 181 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVP 240

Query: 241 SARVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHA 300
           SARVAMYKVCW S+GC+DMDILAAFDAAI DGVDVISISIGGG F NYSDDSISIGAFHA
Sbjct: 241 SARVAMYKVCWASSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 300

Query: 301 MKKGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTF 360
           MKKGIITVTSAGN GP+AGS+VNHAPWIVTVAAS+IDRKFISPLELGNGKNISGV IN F
Sbjct: 301 MKKGIITVTSAGNGGPMAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 360

Query: 361 NPKVKMYPLVSGRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLG 420
           NPK KMYPLVSG DVARNSESK++AS+C E+SLD  KVKG+LV CK LTWG DSV+KS+G
Sbjct: 361 NPKKKMYPLVSGGDVARNSESKDTASFCLEDSLDPTKVKGSLVFCKLLTWGADSVIKSVG 420

Query: 421 GSGVIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPIV 480
            +GVIIQSDQFLDNADIFMAPAT+VSS VG  I  YIKST+TP AVIY+TRQ KA AP+V
Sbjct: 421 ANGVIIQSDQFLDNADIFMAPATMVSSSVGTIIYTYIKSTRTPTAVIYKTRQLKAKAPMV 480

Query: 481 ASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACP 540
           ASFSSRGPNPGS+RILKPDIAAPGV+ILAGYTPLKSLTG KGDTQ+SKFT+MSGTSMACP
Sbjct: 481 ASFSSRGPNPGSHRILKPDIAAPGVNILAGYTPLKSLTGQKGDTQYSKFTIMSGTSMACP 540

Query: 541 HVAAAAAYVKSFHPHWSPAAVRSALITTARPISRRLNPDGEFAYGAGNLNPSQAKNPGLI 600
           HVAAAAAYVKSFHP WSPAA+RSAL+TT RPISRRLNP+GEFAYGAGNLNPS+A +PGLI
Sbjct: 541 HVAAAAAYVKSFHPLWSPAAIRSALLTTTRPISRRLNPEGEFAYGAGNLNPSKAISPGLI 600

Query: 601 YDLNEMSYIQFLCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQRP 660
           YDLNEMSYIQFLC EGYT SSIA+LAGTKSINCSTLIPG GHDSLNYPT QL+L++T++P
Sbjct: 601 YDLNEMSYIQFLCSEGYTGSSIAVLAGTKSINCSTLIPGHGHDSLNYPTFQLSLRSTKQP 660

Query: 661 MTTVFRRRVTNVGRAVSVYNATIRAPPGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPS 720
           MTT FRR+VTNVG+ VSVYNATI+APPGV+ITV PTTLSFSRLLQKRSFK+VVKASPL S
Sbjct: 661 MTTTFRRQVTNVGQPVSVYNATIKAPPGVKITVTPTTLSFSRLLQKRSFKVVVKASPLQS 720

Query: 721 TKMVSGSLAWVGARHVVRSPIVVYSP 744
            KMVSGSLAWVG RHVVRSPIVVYSP
Sbjct: 721 AKMVSGSLAWVGDRHVVRSPIVVYSP 746

BLAST of Spg028673 vs. ExPASy TrEMBL
Match: A0A1S3C036 (subtilisin-like protease SBT4.14 OS=Cucumis melo OX=3656 GN=LOC103495082 PE=3 SV=1)

HSP 1 Score: 1238.8 bits (3204), Expect = 0.0e+00
Identity = 624/746 (83.65%), Postives = 681/746 (91.29%), Query Frame = 0

Query: 1   MSILKSSRLLVFVLFFVVAFIAA---VEFDEEKKHFIVFLENKPVLNEVDAVETHLNVLM 60
           MS+ KSSRLLVF LF +V  +      + +EEK HFIVFL+NKPVLNEVDAVETHL+VLM
Sbjct: 1   MSLSKSSRLLVFALFIIVGCVVQGMDRDENEEKNHFIVFLQNKPVLNEVDAVETHLSVLM 60

Query: 61  SVKESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWD 120
           SVK+SH EA ESMVYSYTKSFNAFAAKL++ EAK LS R+DVHHVIPN YRKLQTTRSWD
Sbjct: 61  SVKKSHAEASESMVYSYTKSFNAFAAKLSDHEAKLLSTRKDVHHVIPNTYRKLQTTRSWD 120

Query: 121 FIGLPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCN 180
           FIGL  NARR TK ESDI+VGLFDTGITPTA+SF+DDGFGPPPKKWKG+CHHFANFT CN
Sbjct: 121 FIGLSSNARRRTKHESDIIVGLFDTGITPTADSFRDDGFGPPPKKWKGTCHHFANFTACN 180

Query: 181 NKLIGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVP 240
            KLIGARYFKLDG+ DP+DILSP+D DGHGTHTSSTATGNA+AGASLSGLA+GTARGGVP
Sbjct: 181 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVP 240

Query: 241 SARVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHA 300
           SARVAMYKVCW S+GC+DMDILAAFDAAI DGVDVISISIGGG F NYSDDSISIGAFHA
Sbjct: 241 SARVAMYKVCWASSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 300

Query: 301 MKKGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTF 360
           MKKGIITVTSAGN GP+AGS+VNHAPWIVTVAAS+IDRKFISPLELGNGKNISGV IN F
Sbjct: 301 MKKGIITVTSAGNGGPMAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 360

Query: 361 NPKVKMYPLVSGRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLG 420
           NPK KMYPLVSG DVARNSESK++AS+C E+SLD  KVKG+LV CK LTWG DSV+KS+G
Sbjct: 361 NPKKKMYPLVSGGDVARNSESKDTASFCLEDSLDPTKVKGSLVFCKLLTWGADSVIKSVG 420

Query: 421 GSGVIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPIV 480
            +GVIIQSDQFLDNADIFMAPAT+VSS VG  I  YIKST+TP AVIY+TRQ KA AP+V
Sbjct: 421 ANGVIIQSDQFLDNADIFMAPATMVSSSVGTIIYTYIKSTRTPTAVIYKTRQLKAKAPMV 480

Query: 481 ASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACP 540
           ASFSSRGPNPGS+RILKPDIAAPGV+ILAGYTPLKSLTG KGDTQ+SKFT+MSGTSMACP
Sbjct: 481 ASFSSRGPNPGSHRILKPDIAAPGVNILAGYTPLKSLTGQKGDTQYSKFTIMSGTSMACP 540

Query: 541 HVAAAAAYVKSFHPHWSPAAVRSALITTARPISRRLNPDGEFAYGAGNLNPSQAKNPGLI 600
           HVAAAAAYVKSFHP WSPAA+RSAL+TT RPISRRLNP+GEFAYGAGNLNPS+A +PGLI
Sbjct: 541 HVAAAAAYVKSFHPLWSPAAIRSALLTTTRPISRRLNPEGEFAYGAGNLNPSKAISPGLI 600

Query: 601 YDLNEMSYIQFLCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQRP 660
           YDLNEMSYIQFLC EGYT SSIA+LAGTKSINCSTLIPG GHDSLNYPT QL+L++T++P
Sbjct: 601 YDLNEMSYIQFLCSEGYTGSSIAVLAGTKSINCSTLIPGHGHDSLNYPTFQLSLRSTKQP 660

Query: 661 MTTVFRRRVTNVGRAVSVYNATIRAPPGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPS 720
           MTT FRR+VTNVG+ VSVYNATI+APPGV+ITV PTTLSFSRLLQKRSFK+VVKASPL S
Sbjct: 661 MTTTFRRQVTNVGQPVSVYNATIKAPPGVKITVTPTTLSFSRLLQKRSFKVVVKASPLQS 720

Query: 721 TKMVSGSLAWVGARHVVRSPIVVYSP 744
            KMVSGSLAWVG RHVVRSPIVVYSP
Sbjct: 721 AKMVSGSLAWVGDRHVVRSPIVVYSP 746

BLAST of Spg028673 vs. ExPASy TrEMBL
Match: A0A6J1FLU9 (subtilisin-like protease SBT4.14 OS=Cucurbita moschata OX=3662 GN=LOC111446986 PE=3 SV=1)

HSP 1 Score: 1225.7 bits (3170), Expect = 0.0e+00
Identity = 622/743 (83.71%), Postives = 672/743 (90.44%), Query Frame = 0

Query: 1   MSILKSSRLLVFVLFFVVAFIAAVEFDEEKKHFIVFLENKPVLNEVDAVETHLNVLMSVK 60
           M I  SSR+LV VL FV+  + AVE DEEKKHFIVFLE KP LNE DAVETHLNVLMSVK
Sbjct: 1   MLISISSRVLVCVLLFVIGCVGAVEIDEEKKHFIVFLEMKPDLNEFDAVETHLNVLMSVK 60

Query: 61  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWDFIG 120
           ES+VEA+ESMVYSYTKSFNAFAAKLTE+EA  LSKREDVHHVIPN+YRKLQTTRSWDFI 
Sbjct: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEDEALALSKREDVHHVIPNRYRKLQTTRSWDFIE 120

Query: 121 LPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 180
           LP  ARRN+K E+DI+VGLFDTGITPTA+SFKDDGFGPPPKKWKG+CHHFANFTGCN KL
Sbjct: 121 LPSKARRNSK-ETDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTGCNQKL 180

Query: 181 IGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVPSAR 240
           IGARYFKLDG+ DP DILSPIDVDGHGTHTSSTATGNA+AGASLSGLAKGTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSAR 240

Query: 241 VAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHAMKK 300
           VAMYKVCW STGCADMDILAAFDAAIHDGVDVISISIGGG+FGNYSDDSISIGAFHA+KK
Sbjct: 241 VAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHALKK 300

Query: 301 GIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTFNPK 360
           GIITVTSAGN GP  GS+VNHAPWIVTV ASAIDRKFIS LELGNGKNISGV INTFNPK
Sbjct: 301 GIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINTFNPK 360

Query: 361 VKMYPLVSGRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLGGSG 420
            KMYPLV G DVAR +E++ESAS+CAE+SLD +KVKG+LV C+ +TWG DSVV +LG +G
Sbjct: 361 KKMYPLVYGGDVARGAENRESASHCAEDSLDPSKVKGSLVFCELITWGVDSVVNALGANG 420

Query: 421 VIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPIVASF 480
            IIQSD++LDNA+IFMAPAT+VSS VG  I +YIKST+TP AVIY+TRQ KA AP+ ASF
Sbjct: 421 AIIQSDEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVA 540
           SSRGPNPG+ RILKPDIAAPGVDILAGYTPLKSLTG +GDTQFSKFTLMSGTSMACPHVA
Sbjct: 481 SSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPHWSPAAVRSALITTARPISRRLNPDGEFAYGAGNLNPSQAKNPGLIYDL 600
           AAAAYVKSFHP WSP A+RSALITTA  ISR+LNPDGEFAYGAGNLNPS+A NPGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPGAIRSALITTASRISRQLNPDGEFAYGAGNLNPSRAINPGLIYDL 600

Query: 601 NEMSYIQFLCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQRPMTT 660
           NEMSYIQFLC EGYT SSI +LAGTKSINCSTLIPG GHDSLNYPT QL LK+ QR M+T
Sbjct: 601 NEMSYIQFLCSEGYTGSSIGVLAGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPQRAMST 660

Query: 661 VFRRRVTNVGRAVSVYNATIRAPPGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKM 720
           VFRRRVTNVG  VSVYNATI+APPGVEITV P+TLSFS+L QKRSFK+ VKA PL S KM
Sbjct: 661 VFRRRVTNVGHPVSVYNATIKAPPGVEITVTPSTLSFSQLQQKRSFKVAVKAGPLASGKM 720

Query: 721 VSGSLAWVGARHVVRSPIVVYSP 744
           VSGS+AW+GARH VRSPIVVYSP
Sbjct: 721 VSGSVAWIGARHAVRSPIVVYSP 742

BLAST of Spg028673 vs. ExPASy TrEMBL
Match: A0A6J1IN55 (subtilisin-like protease SBT4.14 OS=Cucurbita maxima OX=3661 GN=LOC111477941 PE=3 SV=1)

HSP 1 Score: 1214.1 bits (3140), Expect = 0.0e+00
Identity = 618/743 (83.18%), Postives = 669/743 (90.04%), Query Frame = 0

Query: 1   MSILKSSRLLVFVLFFVVAFIAAVEFDEEKKHFIVFLENKPVLNEVDAVETHLNVLMSVK 60
           M I  SSR+LV VL FVV  + AVE DEEKKHFIVFLE KP LNE DAVETHLNVLMSVK
Sbjct: 1   MLISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK 60

Query: 61  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWDFIG 120
           ES+VEA+ESMVYSYTKSFNAFAAKLTEEEA  LSKREDVHHVIPN+YRKLQTTRSWDFIG
Sbjct: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIG 120

Query: 121 LPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 180
           L  +A+RN+K E+DI+VGLFDTGITPTA+SFKDDGFGPPP KWKG+CHHFANFTGCN KL
Sbjct: 121 LSSHAKRNSK-ETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKL 180

Query: 181 IGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVPSAR 240
           IGARYFKLDG++DP DILSPIDVDGHGTHTSSTATGNA+AGASLSGLAKGTARGGVPSAR
Sbjct: 181 IGARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSAR 240

Query: 241 VAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHAMKK 300
           VAMYKVCW STGCADMDILAAFDAAIHDGVDVISISIGGG+FGNYSDDSISIGAFHAMKK
Sbjct: 241 VAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKK 300

Query: 301 GIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTFNPK 360
           GIITVTSAGN GP  GS+VNHAPWIVTV ASAIDRKFIS LELGNGKNISGV IN FNPK
Sbjct: 301 GIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPK 360

Query: 361 VKMYPLVSGRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLGGSG 420
            KMYPLV G DVAR + ++ESAS+C E+SLD +KVKG+LV C+ +TWG DSVV +LG +G
Sbjct: 361 KKMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANG 420

Query: 421 VIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPIVASF 480
            IIQS+++LDNA+IFMAPAT+VSS VG  I +YIKST+TP AVIY+TRQ KA AP+ ASF
Sbjct: 421 AIIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVA 540
           SSRGPNPG+ RILKPDIAAPGVDILAGYTPLKSLTG +GDTQFSKFTLMSGTSMACPHVA
Sbjct: 481 SSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPHWSPAAVRSALITTARPISRRLNPDGEFAYGAGNLNPSQAKNPGLIYDL 600
           AAAAYVKSFHP WSP A+RSALITTA  ISRRLNPDGEFAYGAGNLNPS+A NPGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDL 600

Query: 601 NEMSYIQFLCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQRPMTT 660
           NEMSYIQFLC EGYT  SI++L GTKSINCSTLIPG GHDSLNYPT QL LK+ +R M+T
Sbjct: 601 NEMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMST 660

Query: 661 VFRRRVTNVGRAVSVYNATIRAPPGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKM 720
           VFRRRVTNV   VSVYNATI+APPGVEITV P+TLSFSRL QKRSFK+ VKASPL S KM
Sbjct: 661 VFRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKM 720

Query: 721 VSGSLAWVGARHVVRSPIVVYSP 744
           VSGS+AW+GARH VRSPIVVYSP
Sbjct: 721 VSGSVAWIGARHAVRSPIVVYSP 742

BLAST of Spg028673 vs. TAIR 10
Match: AT4G00230.1 (xylem serine peptidase 1 )

HSP 1 Score: 913.3 bits (2359), Expect = 1.3e-265
Identity = 452/735 (61.50%), Postives = 571/735 (77.69%), Query Frame = 0

Query: 9   LLVFVLFFVVAFIAAVEFDEEKKHFIVFLENKPVLNEVDAVETHLNVLMSVKESHVEAKE 68
           +LV V+ ++    A+ E +  K  +I++L ++P  N  + ++TH+N+L S+  S  EAKE
Sbjct: 14  VLVMVVLWISPRYASAEDEHAKDFYIIYLGDRPD-NTEETIKTHINLLSSLNISQEEAKE 73

Query: 69  SMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWDFIGLPINARRN 128
             VYSYTK+FNAFAAKL+  EAK + + E+V  V  N+YRKL TT+SWDF+GLP+ A+R+
Sbjct: 74  RKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLTAKRH 133

Query: 129 TKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKLIGARYFKL 188
            K E D+++G+ DTGITP +ESF D G GPPP KWKGSC  + NFTGCNNK+IGA+YFK 
Sbjct: 134 LKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKH 193

Query: 189 DGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVPSARVAMYKVCW 248
           DG+    ++ SPID+DGHGTHTSST  G  +A ASL G+A GTARG VPSAR+AMYKVCW
Sbjct: 194 DGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCW 253

Query: 249 ESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHAMKKGIITVTSA 308
             +GCADMDILA F+AAIHDGV++ISISI GG   +YS DSIS+G+FHAM+KGI+TV SA
Sbjct: 254 ARSGCADMDILAGFEAAIHDGVEIISISI-GGPIADYSSDSISVGSFHAMRKGILTVASA 313

Query: 309 GNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTFNPKVKMYPLVS 368
           GN GP +G++ NH PWI+TVAAS IDR F S ++LGNGK+ SG+ I+ F+PK K YPLVS
Sbjct: 314 GNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVS 373

Query: 369 GRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLGGSGVIIQSDQF 428
           G D A+N++ K  A YC  +SLDR KVKG +++C+    G +S +KS GG+G II SDQ+
Sbjct: 374 GVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQY 433

Query: 429 LDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPIVASFSSRGPNPG 488
           LDNA IFMAPAT V+S VGD I  YI ST++  AVI +TRQ    AP VASFSSRGPNPG
Sbjct: 434 LDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASFSSRGPNPG 493

Query: 489 SNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKS 548
           S R+LKPDIAAPG+DILA +T  +SLTGL GDTQFSKFT++SGTSMACPHVA  AAYVKS
Sbjct: 494 SIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVKS 553

Query: 549 FHPHWSPAAVRSALITTARPISRRLNPDGEFAYGAGNLNPSQAKNPGLIYDLNEMSYIQF 608
           FHP W+PAA++SA+IT+A+PISRR+N D EFAYG G +NP +A +PGL+YD++++SY+QF
Sbjct: 554 FHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDISYVQF 613

Query: 609 LCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQRPMTTVFRRRVTN 668
           LC EGY  +++A L GT+S++CS+++PGLGHDSLNYPTIQL L++ +     VFRRRVTN
Sbjct: 614 LCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFRRRVTN 673

Query: 669 VGRAVSVYNATIRAPPGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKMVSGSLAWV 728
           VG   SVY AT+RAP GVEITV P +LSFS+  QKRSFK+VVKA  +   K+VSG L W 
Sbjct: 674 VGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKIVSGLLVWK 733

Query: 729 GARHVVRSPIVVYSP 744
             RH VRSPIV+YSP
Sbjct: 734 SPRHSVRSPIVIYSP 746

BLAST of Spg028673 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 638.6 bits (1646), Expect = 6.0e-183
Identity = 366/767 (47.72%), Postives = 501/767 (65.32%), Query Frame = 0

Query: 3   ILKSSRLLVFVLFFVV---AFIAAVEFDE-EKKHFIVFLENKPVLNEVDAVETHLNVLMS 62
           ++ + R+ +F+L F +   A IAA E +  E+K +IV++      + V+A E H N+LM+
Sbjct: 1   MVSNQRVRLFMLCFCLVNNAVIAATEDENVERKPYIVYMGEATENSLVEAAENHHNLLMT 60

Query: 63  VKESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWDF 122
           V     +A+E  +YSY K+ N F A+L   EA+ LS+ E V  V  N  R+L TTRSWDF
Sbjct: 61  VIGDESKARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDF 120

Query: 123 IGL-PINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCN 182
           +GL     +R+   ES+I+VG+ DTGI   + SF D G GPPP KWKG C    NFT CN
Sbjct: 121 LGLVESKYKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCN 180

Query: 183 NKLIGARYFKL------DGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGT 242
           NK+IGA+YF +      DG  D     +  D DGHGTHTSST  G +++ ASL G+A GT
Sbjct: 181 NKVIGAKYFHIQSEGLPDGEGD-----TAADHDGHGTHTSSTIAGVSVSSASLFGIANGT 240

Query: 243 ARGGVPSARVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSIS 302
           ARGGVPSAR+A YKVCW+S GC DMD+LAAFD AI DGVD+ISISIGG S   + +D I+
Sbjct: 241 ARGGVPSARIAAYKVCWDS-GCTDMDMLAAFDEAISDGVDIISISIGGASL-PFFEDPIA 300

Query: 303 IGAFHAMKKGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISG 362
           IGAFHAMK+GI+T  SAGN+GP   ++ N APW++TVAA+++DRKF + ++LGNG   SG
Sbjct: 301 IGAFHAMKRGILTTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASG 360

Query: 363 VAINTFNPKVKMYPLVSGRDVAR-NSESKESASYCAEESLDRNKVKGNLVLCKFLT---- 422
           +++N FNP+ KMYPL SG   +  ++      S C   +L  +KV G +V C+       
Sbjct: 361 ISLNGFNPRKKMYPLTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGG 420

Query: 423 ---WGTDSVVKSLGGSGVIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAV 482
               G D VV+SL G+GVI+Q  +  D A   +   + V    G  I  YI STK P+AV
Sbjct: 421 NGGQGQDHVVRSLKGAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAV 480

Query: 483 IYRTRQFKATAPIVASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQF 542
           I++T+  K  AP ++SFS+RGP   S  ILKPDI+APG++ILA Y+ L S+TG   D + 
Sbjct: 481 IFKTKTTKMLAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRR 540

Query: 543 SKFTLMSGTSMACPHVAAAAAYVKSFHPHWSPAAVRSALITTARPISRRLNPDGEFAYGA 602
           + F++MSGTSMACPH AAAAAYVKSFHP WSPAA++SAL+TTA P+  + N + E +YG+
Sbjct: 541 TLFSIMSGTSMACPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGS 600

Query: 603 GNLNPSQAKNPGLIYDLNEMSYIQFLCREGYTDSSIAILAG-------TKSINCSTLIPG 662
           G +NP +A +PGL+YD+ E +Y++FLC+EGY  +SI +L G        K  NC  +  G
Sbjct: 601 GQINPRRAIHPGLVYDITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRG 660

Query: 663 LGHDSLNYPTIQLNLKNTQRPMTTVFRRRVTNVGRAVSVYNATIRAPPGVEITVVPTTLS 722
           LG D LNYP++   + +T+  ++ VF R VTNVG   S Y A + AP G+ + VVP  +S
Sbjct: 661 LGSDGLNYPSLHKQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMS 720

Query: 723 FSRLLQKRSFKIVVKASPLPSTK-MVSGSLAWVGAR-HVVRSPIVVY 742
           F R  +KR+FK+V+      + K +VS S+ W  +R H+VRSPI+++
Sbjct: 721 FERPKEKRNFKVVIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLF 759

BLAST of Spg028673 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 572.8 bits (1475), Expect = 4.1e-163
Identity = 317/695 (45.61%), Postives = 448/695 (64.46%), Query Frame = 0

Query: 52  HLNVLMSVKESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQ 111
           HL++L  +  + + A   +V SY +SFN FAA L++ E++ L   ++V  V P+K  +L 
Sbjct: 15  HLSILQKLVGT-IAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELT 74

Query: 112 TTRSWDFIGLPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFA 171
           TTRSWDF+G    ARR + +ESD++VG+ D+GI P +ESF D+GFGPPPKKWKGSC    
Sbjct: 75  TTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGL 134

Query: 172 NFTGCNNKLIGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGT 231
            F  CNNKLIGAR++            S  D +GHGTHT+STA GNA+  AS  GLA+GT
Sbjct: 135 KF-ACNNKLIGARFYNKFAD-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGT 194

Query: 232 ARGGVPSARVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSIS 291
           ARGGVPSAR+A YKVC+    C D+DILAAFD AI DGVDVISISI      N  + S++
Sbjct: 195 ARGGVPSARIAAYKVCFNR--CNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVA 254

Query: 292 IGAFHAMKKGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISG 351
           IG+FHAM +GIIT  SAGN+GP  GS+ N +PW++TVAAS  DR+FI  + LGNGK ++G
Sbjct: 255 IGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTG 314

Query: 352 VAINTFNPKVKMYPLVSGRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDS 411
           +++NTFN     +P+V G++V+RN  S+  A YC+   +D   VKG +VLC       ++
Sbjct: 315 ISVNTFNLNGTKFPIVYGQNVSRNC-SQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREA 374

Query: 412 VVKSLGGSGVIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQF- 471
            +   G  GVI+Q+    D+A +   PA+ +      +I +YI+S + P+A I RT +  
Sbjct: 375 YL--AGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIV 434

Query: 472 KATAPIVASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKG--DTQFSKFTL 531
              AP V SFSSRGP+     +LKPD++APG++ILA ++P+ S +      D +  ++++
Sbjct: 435 DREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSV 494

Query: 532 MSGTSMACPHVAAAAAYVKSFHPHWSPAAVRSALITTARPISRRLNPDGEFAYGAGNLNP 591
           MSGTSMACPHVA  AAYVKSFHP WSP+A++SA++TTA P++ + NP+ EFAYG+G +NP
Sbjct: 495 MSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINP 554

Query: 592 SQAKNPGLIYDLNEMSYIQFLCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQ 651
           ++A +PGL+Y++    Y++ LC EG+  +++   +G +++ CS          LNYPT+ 
Sbjct: 555 TKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSG-QNVTCSERTE---VKDLNYPTMT 614

Query: 652 LNLKNTQRPMTTVFRRRVTNVGRAVSVYNAT-IRAPPGVEITVVPTTLSFSRLLQKRSFK 711
             + +   P    F+R VTNVG   S Y A+ +   P ++I++ P  L F  L +K+SF 
Sbjct: 615 TFVSSLD-PFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFV 674

Query: 712 IVVKASPLPSTKMVSGSLAWVGARHVVRSPIVVYS 743
           + +    L     VS S+ W    H VRSPIV YS
Sbjct: 675 VTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 690

BLAST of Spg028673 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 562.4 bits (1448), Expect = 5.5e-160
Identity = 326/749 (43.52%), Postives = 478/749 (63.82%), Query Frame = 0

Query: 2   SILKSSRLLVFVLFFVVAFIAAVEFDEEKKHFIVFLENKPVLNEVDAVETHLNVLMSVK- 61
           ++  SS LL  +L   ++ ++AV   ++K+ +IV++ +     +      H+N+L  V  
Sbjct: 3   TLAASSSLLSCLLVLFLSSVSAV--TDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTG 62

Query: 62  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWDFIG 121
           ES +E +  +V SY +SFN FAA+LTE E + ++K   V  V PNK  +LQTT SWDF+G
Sbjct: 63  ESSIEGR--LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMG 122

Query: 122 LP--INARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNN 181
           L   I  +RN   ESD ++G+ D+GITP ++SF D GFGPPP+KWKG C    NFT CNN
Sbjct: 123 LKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFT-CNN 182

Query: 182 KLIGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVPS 241
           KLIGAR +  +G+          D+DGHGTHT+STA GNA+  AS  G+  GT RGGVP+
Sbjct: 183 KLIGARDYTSEGTR---------DMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPA 242

Query: 242 ARVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHAM 301
           +RVA YKVC   TGC+   +L+AFD AI DGVD+I+ISIG  +   + +D I+IGAFHAM
Sbjct: 243 SRVAAYKVC-TPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAM 302

Query: 302 KKGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTFN 361
            KG++TV SAGNSGP   S+   APWI+TVAAS  +R F++ + LGNGK + G ++N + 
Sbjct: 303 AKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYE 362

Query: 362 PKVKMYPLVSGRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLGG 421
            K K YPLV G+  A ++   ESA  C    +D+++VKG +++C     G   +V+S+G 
Sbjct: 363 MKGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCG--GPGGLKIVESVGA 422

Query: 422 SGVIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQ-FKATAPIV 481
            G+I ++ +  D A I   PA  + +   +++ +Y++ST +P+A++ +T   F  T+P++
Sbjct: 423 VGLIYRTPK-PDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVI 482

Query: 482 ASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACP 541
           ASFSSRGPN  +  ILKPDI APGV+ILA Y+P    +  + DT+  K++++SGTSM+CP
Sbjct: 483 ASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCP 542

Query: 542 HVAAAAAYVKSFHPHWSPAAVRSALITTARPISRRLN--PDGEFAYGAGNLNPSQAKNPG 601
           HVA  AAYVK+F+P WSP+ ++SA++TTA P++         EFAYG+G+++P  A NPG
Sbjct: 543 HVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPG 602

Query: 602 LIYDLNEMSYIQFLCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQ 661
           L+Y+L++  +I FLC   YT   + +++G +++ CS     L   +LNYP++   L  + 
Sbjct: 603 LVYELDKSDHIAFLCGMNYTSQVLKVISG-ETVTCSEAKKILPR-NLNYPSMSAKLSGSG 662

Query: 662 RPMTTVFRRRVTNVGRAVSVYNATIRAPPG--VEITVVPTTLSFSRLLQKRSFKIVVKAS 721
              T  F R +TNVG   S Y + + A  G  +++ + P+ LSF  + +K+SF + V  S
Sbjct: 663 TTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGS 722

Query: 722 PLPSTKMVSGSLAWVGARHVVRSPIVVYS 743
            L S    S +L W    H VRSPIVVY+
Sbjct: 723 NLDSEVPSSANLIWSDGTHNVRSPIVVYT 729

BLAST of Spg028673 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 553.5 bits (1425), Expect = 2.5e-157
Identity = 322/742 (43.40%), Postives = 466/742 (62.80%), Query Frame = 0

Query: 11  VFVLFFVVAFIAAVEFDEEKKHFIVFLENKPVLNEVDAVETHLNVLMSVK-ESHVEAKES 70
           +F L  VV+F +A + D++K+ +IV++   P   +   +  H ++L  V  ES ++  + 
Sbjct: 13  IFAL-LVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQ--DR 72

Query: 71  MVYSYTKSFNAFAAKLTEEEAKTLSKREDVHHVIPNKYRKLQTTRSWDFIGLPINAR--R 130
           +V +Y +SFN FAA+LTE E + L+  ++V  V P+K   LQTT SW+F+GL    R  R
Sbjct: 73  LVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKR 132

Query: 131 NTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKLIGARYF- 190
           N   ESD ++G+ D+GI P ++SF   GFGPPPKKWKG C    NFT CNNKLIGARY+ 
Sbjct: 133 NPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFT-CNNKLIGARYYT 192

Query: 191 -KLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSGLAKGTARGGVPSARVAMYK 250
            KL+G  +     S  D  GHG+HT+S A GNA+   S  GL  GT RGGVP+AR+A+YK
Sbjct: 193 PKLEGFPE-----SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYK 252

Query: 251 VCWEST-GCADMDILAAFDAAIHDGVDVISISIGGGSFGNYSDDSISIGAFHAMKKGIIT 310
           VC      C    ILAAFD AI D VD+I++S+G  + G + +D+++IGAFHAM KGI+T
Sbjct: 253 VCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILT 312

Query: 311 VTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVAINTFNPKVKMY 370
           V  AGN+GP   +IV+ APW+ TVAAS ++R FI+ + LGNGK I G ++N+F+   K Y
Sbjct: 313 VNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKY 372

Query: 371 PLVSGRDVARNSESKESASYCAEESLDRNKVKGNLVLCKFLTWGTDSVVKSLGGSGVIIQ 430
           PLV G+  +   ++  SA +C+   LD  +VKG +VLC   T       +++G    I++
Sbjct: 373 PLVYGKSASSRCDA-SSAGFCSPGCLDSKRVKGKIVLCD--TQRNPGEAQAMGAVASIVR 432

Query: 431 SDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQ-FKATAPIVASFSSR 490
            + + D A +F  P +V+S    + + +Y+ STK PKA + ++   F   AP+VAS+SSR
Sbjct: 433 -NPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSR 492

Query: 491 GPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAA 550
           GPNP  + ILKPDI APG +ILA Y+P   +   + DT+  K+T++SGTSM+CPHVA  A
Sbjct: 493 GPNPLIHDILKPDITAPGSEILAAYSPY--VPPSESDTRHVKYTVISGTSMSCPHVAGVA 552

Query: 551 AYVKSFHPHWSPAAVRSALITTARPISRRLNPD---GEFAYGAGNLNPSQAKNPGLIYDL 610
           AY+K+FHP WSP+ ++SA++TTA P++   +P     EFAYGAG+++P  A +PGL+Y+ 
Sbjct: 553 AYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEA 612

Query: 611 NEMSYIQFLCREGYTDSSIAILAGTKSINCSTLIPGLGHDSLNYPTIQLNLKNTQRPMTT 670
           N+  +I FLC   YT   + +++G  S +C+         +LNYP++   +  T +P   
Sbjct: 613 NKSDHITFLCGFNYTGKKLRLISGDSS-SCTKEQTKSLTRNLNYPSMSAQVSGT-KPFKV 672

Query: 671 VFRRRVTNVGRAVSVYNATIRAPPGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKM 730
            FRR VTNVGR  + Y A +     +++ VVP  LS   L +K+SF + V  +   +  +
Sbjct: 673 TFRRTVTNVGRPNATYKAKV-VGSKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENL 732

Query: 731 VSGSLAWVGARHVVRSPIVVYS 743
           VS  L W    H VRSPIVVY+
Sbjct: 733 VSAQLIWSDGVHFVRSPIVVYA 736