Spg028591 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg028591 vs. NCBI nr
Match: XP_038893247.1 (SAC3 family protein A isoform X1 [Benincasa hispida])

HSP 1 Score: 1837.8 bits (4759), Expect = 0.0e+00
Identity = 936/1013 (92.40%), Postives = 962/1013 (94.97%), Query Frame = 0

Query: 62   MNQDGNTETVPPVQPSSLENQYIGDGNQSVATSTYLPLTSAPEAISWANHKVDGSSNENG 121
            MNQ GNTET+ P QPSSLENQ++GDGNQSVATSTYLP+TSAPEAI+WANHKVDGSSNENG
Sbjct: 1    MNQGGNTETLVPGQPSSLENQHVGDGNQSVATSTYLPMTSAPEAITWANHKVDGSSNENG 60

Query: 122  LLPNSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSADPYGY 181
            LLPNSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYA YSNS DPYGY
Sbjct: 61   LLPNSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAQYSNSTDPYGY 120

Query: 182  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQSLSSFQNTGFYAGSTSYSTTYYNPG 241
            ANAGYQGYYN+YQQQPNHSYSQPVGAYQNTGAPYQ LSS+QNTGFYAGSTSYSTTYYNPG
Sbjct: 121  ANAGYQGYYNSYQQQPNHSYSQPVGAYQNTGAPYQPLSSYQNTGFYAGSTSYSTTYYNPG 180

Query: 242  DYQTAGGYQTSSYSNQTTSWNGGNYGNYVPNQYAQYTPDSSGAYSSSSTNANSLQYQQHY 301
            DYQTAGGY TSSYSNQTTSWNGGNYGNYVPNQYAQYT DSSGAYSSSSTNANSLQYQQH 
Sbjct: 181  DYQTAGGYPTSSYSNQTTSWNGGNYGNYVPNQYAQYTSDSSGAYSSSSTNANSLQYQQHC 240

Query: 302  KQWADYYSQTEVSCAPGTEKLSTPGTANAGYPVHGSTSYPAPSSQPPPSYTPSWRPESSS 361
            KQWADYYSQTEVSCAPGTEKLSTP TANAGYPVHGS +YPAP+SQPPPSYTPSWRPES S
Sbjct: 241  KQWADYYSQTEVSCAPGTEKLSTPVTANAGYPVHGSANYPAPNSQPPPSYTPSWRPESGS 300

Query: 362  SELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLDLKTSYDSFQGQQKSAG 421
            SELVSAQPGAVSS NHDGYWKHGAPNSQVHLTNATQPHFEKPLDLK SY+SFQ QQ SAG
Sbjct: 301  SELVSAQPGAVSSDNHDGYWKHGAPNSQVHLTNATQPHFEKPLDLKNSYNSFQDQQNSAG 360

Query: 422  PQGPNLQYPAHLAPQSYQLPSQSVPSLEARRTKLQIPTNPRIASNLSILKTSKDSSTVDA 481
            PQGPNLQYPAHLAPQSYQLPSQSVP +EARRTKLQIPTNPRIASNLSILK SKDSST DA
Sbjct: 361  PQGPNLQYPAHLAPQSYQLPSQSVPPVEARRTKLQIPTNPRIASNLSILKASKDSSTADA 420

Query: 482  AVQPAYVSVSMPKPNEKELSNDTAEAVLKPGMFPKSLRGYVERALARCKDEKLMTSCQSV 541
            AVQPAYVSVS+PKPNEKELSNDTAE+VLKPGMFPKSLRGYVERALARCKDEKLMTSCQSV
Sbjct: 421  AVQPAYVSVSLPKPNEKELSNDTAESVLKPGMFPKSLRGYVERALARCKDEKLMTSCQSV 480

Query: 542  LKEMITKATADGTLYTKDWDIEPLFPLPSADAVNTEYYFSLAKLNNLQGSSPLSSLSKPK 601
            LKEMITKATADGTLYTKDWD+EPLFPLPSADAVNT+         NLQ S+PLSS SK K
Sbjct: 481  LKEMITKATADGTLYTKDWDVEPLFPLPSADAVNTD---------NLQVSTPLSSSSKSK 540

Query: 602  RSPSRRSKSRWEPLPVEKPAEAHPPYSNGAAAKYDGWANVSERDKKTLSGNSETKNVVNS 661
            RSPSRRSKSRWEPLPVEKPAEA PPYSNGAA KY GWANVSERDKKTLSGNSETK+V NS
Sbjct: 541  RSPSRRSKSRWEPLPVEKPAEAPPPYSNGAAEKYGGWANVSERDKKTLSGNSETKDVSNS 600

Query: 662  RFPLWDQRTVGKISQGPAKKQRVADGSAPDNDGASSDSDKEQSLTAYYSGAMALANSPEE 721
            RFPLWDQRTVGKISQGPAKKQRVADGSAPDN GASSDSDKEQSLTAYYSGAMALANSPEE
Sbjct: 601  RFPLWDQRTVGKISQGPAKKQRVADGSAPDNGGASSDSDKEQSLTAYYSGAMALANSPEE 660

Query: 722  KKKRENRSKRFEKGHGHRGENNHFKPKNTGTGNLYTRRASALVLGKNLENGGCRAVEDID 781
            KKKRENRSKRFEKGHGHRGENN FKPKN G G+LYTRRASALV+GK+LE+GGCRAVEDID
Sbjct: 661  KKKRENRSKRFEKGHGHRGENNLFKPKNAGIGSLYTRRASALVIGKSLEDGGCRAVEDID 720

Query: 782  WDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVQMSQKNYLYKCDQLKSIR 841
            WDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVQMSQKNYLYKCDQLKSIR
Sbjct: 721  WDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVQMSQKNYLYKCDQLKSIR 780

Query: 842  QDLTVQRIRNQLTAKVYEIHARLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYNL 901
            QDLTVQRIRNQLTAKVYE H RLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYNL
Sbjct: 781  QDLTVQRIRNQLTAKVYETHGRLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYNL 840

Query: 902  LCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRSAVTSGNYVKFFRLHKAAPNLNA 961
            LCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAV +AVTSGNYVKFFRL+KAAPNLNA
Sbjct: 841  LCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVSAAVTSGNYVKFFRLYKAAPNLNA 900

Query: 962  CLMDLYAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGFFTSSG------DVDGLEECTEWL 1021
            CLMDLYAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGF +SSG      DVDG+EECTEWL
Sbjct: 901  CLMDLYAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGFSSSSGDEVRDKDVDGVEECTEWL 960

Query: 1022 KGHGACLITDNNGEMQLDTKASSTTLYMPEPEDAVAHGDANLAVNDFFTRTSS 1069
            KGHGACLI DNNGEMQLD KASSTTLYMPEP+DAVAHGDANLAVNDFFTRTSS
Sbjct: 961  KGHGACLIADNNGEMQLDAKASSTTLYMPEPDDAVAHGDANLAVNDFFTRTSS 1004

BLAST of Spg028591 vs. NCBI nr
Match: KAG7031833.1 (SAC3 family protein A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1830.5 bits (4740), Expect = 0.0e+00
Identity = 932/1014 (91.91%), Postives = 960/1014 (94.67%), Query Frame = 0

Query: 62   MNQDGNTETVPPVQPSSLENQYIGDGNQSVATSTYLPLTSAPEAISWANHKVDGSSNENG 121
            MNQ GNTET+ P QPSSLENQ+IGDGNQS  TSTYLPLTSAPEAI+WANHKVDGSSNENG
Sbjct: 1    MNQGGNTETLVPAQPSSLENQHIGDGNQSATTSTYLPLTSAPEAIAWANHKVDGSSNENG 60

Query: 122  LLPNSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSADPYGY 181
            LLPNST+QYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNS+DPYGY
Sbjct: 61   LLPNSTFQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSSDPYGY 120

Query: 182  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQSLSSFQNTGFYAGSTSYSTTYYNPG 241
            ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQ LSS+QNTGFYAGSTSYSTTYYNPG
Sbjct: 121  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQPLSSYQNTGFYAGSTSYSTTYYNPG 180

Query: 242  DYQTAGGYQTSSYSNQTT-SWNGGNYGNYVPNQYAQYTPDSSGAYSSSSTNANSLQYQQH 301
            DYQTAGGY TSSYSNQTT SWNGGNYGNYVPNQY+QYT DSSGAYSSSSTNANSLQYQQH
Sbjct: 181  DYQTAGGYPTSSYSNQTTSSWNGGNYGNYVPNQYSQYTTDSSGAYSSSSTNANSLQYQQH 240

Query: 302  YKQWADYYSQTEVSCAPGTEKLSTPGTANAGYPVHGSTSYPAPSSQPPPSYTPSWRPESS 361
            YKQWADYYSQTEVSCAPGTEKLS PGTANAGY VHGST+YPAP+SQPPPSYTPSWRPES 
Sbjct: 241  YKQWADYYSQTEVSCAPGTEKLSAPGTANAGYQVHGSTNYPAPNSQPPPSYTPSWRPESG 300

Query: 362  SSELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLDLKTSYDSFQGQQKSA 421
            SSELVSAQPGAVSSGNHDGYWKHG PNSQVHLTNATQPHFEKPLDLKTSYDSFQ QQKSA
Sbjct: 301  SSELVSAQPGAVSSGNHDGYWKHGTPNSQVHLTNATQPHFEKPLDLKTSYDSFQDQQKSA 360

Query: 422  GPQGPNLQYPAHLAPQSYQLPSQSVPSLEARRTKLQIPTNPRIASNLSILKTSKDSSTVD 481
            GPQGP+LQYPAHLAPQSYQLPSQSVP LEARRTKLQIPTNPRIASNLSILKTSKDSST D
Sbjct: 361  GPQGPDLQYPAHLAPQSYQLPSQSVPPLEARRTKLQIPTNPRIASNLSILKTSKDSSTAD 420

Query: 482  AAVQPAYVSVSMPKPNEKELSNDTAEAVLKPGMFPKSLRGYVERALARCKDEKLMTSCQS 541
            AAVQPAYVSV +PKPNEKELSNDTAE+VLKPGMFPKSLRGYVERALARCKDEKLMTSCQS
Sbjct: 421  AAVQPAYVSVLLPKPNEKELSNDTAESVLKPGMFPKSLRGYVERALARCKDEKLMTSCQS 480

Query: 542  VLKEMITKATADGTLYTKDWDIEPLFPLPSADAVNTEYYFSLAKLNNLQGSSPLSSLSKP 601
            VLKEMITKATADGTLYTKDWD+EPLFPLPSADAVN++         NL GS+PLSSLSK 
Sbjct: 481  VLKEMITKATADGTLYTKDWDVEPLFPLPSADAVNSD---------NLHGSTPLSSLSKS 540

Query: 602  KRSPSRRSKSRWEPLPVEKPAEAHPPYSNGAAAKYDGWANVSERDKKTLSGNSETKNVVN 661
            KRSPSRRSKSRWEPLPVEKPAEA P YSNGAA KY GWANVSERDKK LSGNSETK+V N
Sbjct: 541  KRSPSRRSKSRWEPLPVEKPAEAPPSYSNGAATKYGGWANVSERDKKNLSGNSETKDVSN 600

Query: 662  SRFPLWDQRTVGKISQGPAKKQRVADGSAPDNDGASSDSDKEQSLTAYYSGAMALANSPE 721
            SRF LW+QRTVGKISQGPAKKQRV DGSA DNDGAS DSDKEQSLTAYYSGAMALANSPE
Sbjct: 601  SRFTLWNQRTVGKISQGPAKKQRVLDGSALDNDGASGDSDKEQSLTAYYSGAMALANSPE 660

Query: 722  EKKKRENRSKRFEKGHGHRGENNHFKPKNTGTGNLYTRRASALVLGKNLENGGCRAVEDI 781
            EK+KRE RSKRFEKGHGHRGENNHFKPKN G G+LYTRRASALVLGKNLE+GGCRAVEDI
Sbjct: 661  EKRKRETRSKRFEKGHGHRGENNHFKPKNAGIGSLYTRRASALVLGKNLEDGGCRAVEDI 720

Query: 782  DWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVQMSQKNYLYKCDQLKSI 841
            DWDALTIKGTCQEIEKRYLRLTSAPDP SVRPEEVLEK+LNMVQMSQKNYLYKCDQLKSI
Sbjct: 721  DWDALTIKGTCQEIEKRYLRLTSAPDPCSVRPEEVLEKSLNMVQMSQKNYLYKCDQLKSI 780

Query: 842  RQDLTVQRIRNQLTAKVYEIHARLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYN 901
            RQDLTVQRIRNQLTAKVYE H RLALEVGDLPEYNQCQSQL TLYAEGIEGC+MEFAAYN
Sbjct: 781  RQDLTVQRIRNQLTAKVYETHGRLALEVGDLPEYNQCQSQLTTLYAEGIEGCYMEFAAYN 840

Query: 902  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRSAVTSGNYVKFFRLHKAAPNLN 961
            LLCAILHSNNKRDLLSLMSRLSDQAKKDVAV+HALAVR+AVTSGNYVKFFRL+KAAPNLN
Sbjct: 841  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVSHALAVRAAVTSGNYVKFFRLYKAAPNLN 900

Query: 962  ACLMDLYAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGFFTSSG------DVDGLEECTEW 1021
            ACLMDLY+EKMRYKA+NCMSRSYRPSLPVPYIAQVLGF TSSG      DVDGLEECTEW
Sbjct: 901  ACLMDLYSEKMRYKALNCMSRSYRPSLPVPYIAQVLGFSTSSGDEVRDKDVDGLEECTEW 960

Query: 1022 LKGHGACLITDNNGEMQLDTKASSTTLYMPEPEDAVAHGDANLAVNDFFTRTSS 1069
            LKGHGACLITDNNGEMQLD KASSTTLYMPEPEDAVAHGDANLAVNDFFTR SS
Sbjct: 961  LKGHGACLITDNNGEMQLDAKASSTTLYMPEPEDAVAHGDANLAVNDFFTRASS 1005

BLAST of Spg028591 vs. NCBI nr
Match: XP_022956467.1 (SAC3 family protein A-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1829.3 bits (4737), Expect = 0.0e+00
Identity = 933/1014 (92.01%), Postives = 960/1014 (94.67%), Query Frame = 0

Query: 62   MNQDGNTETVPPVQPSSLENQYIGDGNQSVATSTYLPLTSAPEAISWANHKVDGSSNENG 121
            MNQ GNTET+ P QPSSLENQ+IGDGNQS  TSTYLPLTSAPEAI+WANHKVDGSSNENG
Sbjct: 1    MNQGGNTETLVPAQPSSLENQHIGDGNQSATTSTYLPLTSAPEAIAWANHKVDGSSNENG 60

Query: 122  LLPNSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSADPYGY 181
            LLPNST+QYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNS+DPYGY
Sbjct: 61   LLPNSTFQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSSDPYGY 120

Query: 182  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQSLSSFQNTGFYAGSTSYSTTYYNPG 241
            ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQ LSS+QNTGFYAGSTSYSTTYYNPG
Sbjct: 121  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQPLSSYQNTGFYAGSTSYSTTYYNPG 180

Query: 242  DYQTAGGYQTSSYSNQTT-SWNGGNYGNYVPNQYAQYTPDSSGAYSSSSTNANSLQYQQH 301
            DYQTAGGY TSSYSNQTT SWNGGNYGNYVPNQY+QYT DSSGAYSSSSTNANSLQYQQH
Sbjct: 181  DYQTAGGYPTSSYSNQTTSSWNGGNYGNYVPNQYSQYTTDSSGAYSSSSTNANSLQYQQH 240

Query: 302  YKQWADYYSQTEVSCAPGTEKLSTPGTANAGYPVHGSTSYPAPSSQPPPSYTPSWRPESS 361
            YKQWADYYSQTEVSCAPGTEKLS PGTANAGY VHGST+YPAP+SQPPPSYTPSWRPES 
Sbjct: 241  YKQWADYYSQTEVSCAPGTEKLSAPGTANAGYQVHGSTNYPAPNSQPPPSYTPSWRPESG 300

Query: 362  SSELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLDLKTSYDSFQGQQKSA 421
            SSELVSAQPGAVSSGNHDGYWKHG PNSQVHLTNATQPHFEKPLDLKTSYDSFQ QQKSA
Sbjct: 301  SSELVSAQPGAVSSGNHDGYWKHGTPNSQVHLTNATQPHFEKPLDLKTSYDSFQDQQKSA 360

Query: 422  GPQGPNLQYPAHLAPQSYQLPSQSVPSLEARRTKLQIPTNPRIASNLSILKTSKDSSTVD 481
            GPQGPNLQYPAHLAPQSYQLPSQSVP LEARRTKLQIPTNPRIASNLSILKTSKD ST D
Sbjct: 361  GPQGPNLQYPAHLAPQSYQLPSQSVPPLEARRTKLQIPTNPRIASNLSILKTSKDISTAD 420

Query: 482  AAVQPAYVSVSMPKPNEKELSNDTAEAVLKPGMFPKSLRGYVERALARCKDEKLMTSCQS 541
            AAVQPAYVSV +PKPNEKELSNDTAE+VLKPGMFPKSLRGYVERALARCKDEKLMTSCQS
Sbjct: 421  AAVQPAYVSVLLPKPNEKELSNDTAESVLKPGMFPKSLRGYVERALARCKDEKLMTSCQS 480

Query: 542  VLKEMITKATADGTLYTKDWDIEPLFPLPSADAVNTEYYFSLAKLNNLQGSSPLSSLSKP 601
            VLKEMITKATADGTLYTKDWD+EPLFPLPSADAVN++         NL GS+PLSSLSK 
Sbjct: 481  VLKEMITKATADGTLYTKDWDVEPLFPLPSADAVNSD---------NLHGSTPLSSLSKS 540

Query: 602  KRSPSRRSKSRWEPLPVEKPAEAHPPYSNGAAAKYDGWANVSERDKKTLSGNSETKNVVN 661
            KRSPSRRSKSRWEPLPVEKPAEA PPYSNGAA KY GWANVSERDKK LSGNSETK+V N
Sbjct: 541  KRSPSRRSKSRWEPLPVEKPAEA-PPYSNGAATKYGGWANVSERDKKNLSGNSETKDVSN 600

Query: 662  SRFPLWDQRTVGKISQGPAKKQRVADGSAPDNDGASSDSDKEQSLTAYYSGAMALANSPE 721
            SRF LW+QRTVGKISQGPAKKQRV DGSA DNDGAS DSDKEQSLTAYYSGAMALANSPE
Sbjct: 601  SRFTLWNQRTVGKISQGPAKKQRVLDGSALDNDGASGDSDKEQSLTAYYSGAMALANSPE 660

Query: 722  EKKKRENRSKRFEKGHGHRGENNHFKPKNTGTGNLYTRRASALVLGKNLENGGCRAVEDI 781
            EK+KRE RSKRFEKGHGHRGENNHFKPKN G G+LYTRRASALVLGKNLE+GGCRAVEDI
Sbjct: 661  EKRKRETRSKRFEKGHGHRGENNHFKPKNAGIGSLYTRRASALVLGKNLEDGGCRAVEDI 720

Query: 782  DWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVQMSQKNYLYKCDQLKSI 841
            DWDALTIKGTCQEIEKRYLRLTSAPDP SVRPEEVLEK+LNMVQMSQKNYLYKCDQLKSI
Sbjct: 721  DWDALTIKGTCQEIEKRYLRLTSAPDPCSVRPEEVLEKSLNMVQMSQKNYLYKCDQLKSI 780

Query: 842  RQDLTVQRIRNQLTAKVYEIHARLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYN 901
            RQDLTVQRIRNQLTAKVYE H RLALEVGDLPEYNQCQSQL TLYAEGIEGC+MEFAAYN
Sbjct: 781  RQDLTVQRIRNQLTAKVYETHGRLALEVGDLPEYNQCQSQLTTLYAEGIEGCYMEFAAYN 840

Query: 902  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRSAVTSGNYVKFFRLHKAAPNLN 961
            LLCAILHSNNKRDLLSLMSRLSDQAKKDVAV+HALAVR+AVTSGNYVKFFRL+KAAPNLN
Sbjct: 841  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVSHALAVRAAVTSGNYVKFFRLYKAAPNLN 900

Query: 962  ACLMDLYAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGFFTSSG------DVDGLEECTEW 1021
            ACLMDLY+EKMRYKA+NCMSRSYRPSLPVPYIAQVLGF TSSG      DVDGLEECTEW
Sbjct: 901  ACLMDLYSEKMRYKALNCMSRSYRPSLPVPYIAQVLGFSTSSGDEVRDKDVDGLEECTEW 960

Query: 1022 LKGHGACLITDNNGEMQLDTKASSTTLYMPEPEDAVAHGDANLAVNDFFTRTSS 1069
            LKGHGACLITDNNGEMQLD KASSTTLYMPEPEDAVAHGDANLAVNDFFTR SS
Sbjct: 961  LKGHGACLITDNNGEMQLDAKASSTTLYMPEPEDAVAHGDANLAVNDFFTRASS 1004

BLAST of Spg028591 vs. NCBI nr
Match: XP_022967361.1 (SAC3 family protein A isoform X1 [Cucurbita maxima])

HSP 1 Score: 1823.5 bits (4722), Expect = 0.0e+00
Identity = 929/1014 (91.62%), Postives = 958/1014 (94.48%), Query Frame = 0

Query: 62   MNQDGNTETVPPVQPSSLENQYIGDGNQSVATSTYLPLTSAPEAISWANHKVDGSSNENG 121
            MNQ GNTET+ P QPSSLENQ+IGDGNQ   TSTYLPLTSAPEA++WANHKVDGSSNENG
Sbjct: 1    MNQGGNTETLVPAQPSSLENQHIGDGNQPATTSTYLPLTSAPEALAWANHKVDGSSNENG 60

Query: 122  LLPNSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSADPYGY 181
            LLPNST+QYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNS+DPYGY
Sbjct: 61   LLPNSTFQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSSDPYGY 120

Query: 182  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQSLSSFQNTGFYAGSTSYSTTYYNPG 241
            ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQ LSS+QNTGFYAGSTSYSTTYYNPG
Sbjct: 121  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQPLSSYQNTGFYAGSTSYSTTYYNPG 180

Query: 242  DYQTAGGYQTSSYSNQTT-SWNGGNYGNYVPNQYAQYTPDSSGAYSSSSTNANSLQYQQH 301
            DYQTAGGY TSSYSNQTT SWNGGNYGNYVPNQY+QYT DSSGAYSSSSTNANSLQYQQH
Sbjct: 181  DYQTAGGYPTSSYSNQTTSSWNGGNYGNYVPNQYSQYTTDSSGAYSSSSTNANSLQYQQH 240

Query: 302  YKQWADYYSQTEVSCAPGTEKLSTPGTANAGYPVHGSTSYPAPSSQPPPSYTPSWRPESS 361
            YKQWADYYSQTEVSCAPGTEKLS PGTANAGY VHGST+YPAP+SQPPPSYTPSWRPES 
Sbjct: 241  YKQWADYYSQTEVSCAPGTEKLSMPGTANAGYQVHGSTNYPAPNSQPPPSYTPSWRPESG 300

Query: 362  SSELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLDLKTSYDSFQGQQKSA 421
            SSELVSAQPGAVSSGNHDGYWKHG PNSQVHLTNATQPHFEKPLDLKTSYDSFQ QQKSA
Sbjct: 301  SSELVSAQPGAVSSGNHDGYWKHGTPNSQVHLTNATQPHFEKPLDLKTSYDSFQDQQKSA 360

Query: 422  GPQGPNLQYPAHLAPQSYQLPSQSVPSLEARRTKLQIPTNPRIASNLSILKTSKDSSTVD 481
            GPQGPNLQYPAHLAPQSYQLPSQSVP LEARRTKLQIPTNPRIASNLSILKTSKDSST D
Sbjct: 361  GPQGPNLQYPAHLAPQSYQLPSQSVPPLEARRTKLQIPTNPRIASNLSILKTSKDSSTAD 420

Query: 482  AAVQPAYVSVSMPKPNEKELSNDTAEAVLKPGMFPKSLRGYVERALARCKDEKLMTSCQS 541
            AAVQPAYVSV +PKPNEKELSNDTAE+VLKPGMFPKSLRGYVERALARCKDEKLMTSCQS
Sbjct: 421  AAVQPAYVSVLLPKPNEKELSNDTAESVLKPGMFPKSLRGYVERALARCKDEKLMTSCQS 480

Query: 542  VLKEMITKATADGTLYTKDWDIEPLFPLPSADAVNTEYYFSLAKLNNLQGSSPLSSLSKP 601
            VLKEMITKATADGTLYTKDWD+EPLFPLPSADAVN++         NL GS+PLSSLSK 
Sbjct: 481  VLKEMITKATADGTLYTKDWDVEPLFPLPSADAVNSD---------NLHGSTPLSSLSKS 540

Query: 602  KRSPSRRSKSRWEPLPVEKPAEAHPPYSNGAAAKYDGWANVSERDKKTLSGNSETKNVVN 661
            KRSPSRRSKSRWEPLPVEKPAEA P YSNGAA KY GWANVSERDKK LSGNSETK+V N
Sbjct: 541  KRSPSRRSKSRWEPLPVEKPAEAPPSYSNGAATKYGGWANVSERDKKNLSGNSETKDVSN 600

Query: 662  SRFPLWDQRTVGKISQGPAKKQRVADGSAPDNDGASSDSDKEQSLTAYYSGAMALANSPE 721
            SRF LW+QRTVGKISQGPAKKQRV DGSA DNDGAS DSDKEQSLTAYYSGAMALANSPE
Sbjct: 601  SRFTLWNQRTVGKISQGPAKKQRVLDGSALDNDGASGDSDKEQSLTAYYSGAMALANSPE 660

Query: 722  EKKKRENRSKRFEKGHGHRGENNHFKPKNTGTGNLYTRRASALVLGKNLENGGCRAVEDI 781
            EK+KRE RSKRFEKGHGHRGENNHFKPKN G G+LYTRRASALVLGKNLE+GGCRAVEDI
Sbjct: 661  EKRKRETRSKRFEKGHGHRGENNHFKPKNAGIGSLYTRRASALVLGKNLEDGGCRAVEDI 720

Query: 782  DWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVQMSQKNYLYKCDQLKSI 841
            DWDALTIKGTCQEIEKRYLRLTSAPDP SVRPEEVLEK+LNMVQMSQKNYLYKCDQLKSI
Sbjct: 721  DWDALTIKGTCQEIEKRYLRLTSAPDPCSVRPEEVLEKSLNMVQMSQKNYLYKCDQLKSI 780

Query: 842  RQDLTVQRIRNQLTAKVYEIHARLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYN 901
            RQDLTVQRIRNQLTAKVYE H RLALEVGDLPEYNQCQSQL TLYAEGIEGC+MEFAAYN
Sbjct: 781  RQDLTVQRIRNQLTAKVYETHGRLALEVGDLPEYNQCQSQLTTLYAEGIEGCYMEFAAYN 840

Query: 902  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRSAVTSGNYVKFFRLHKAAPNLN 961
            LLCAILHSNNKRDLLSLMSRLSDQAKKDVAV+HALAVR+AVTSGNYVKFFRL+KAAPNLN
Sbjct: 841  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVSHALAVRAAVTSGNYVKFFRLYKAAPNLN 900

Query: 962  ACLMDLYAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGFFTSSG------DVDGLEECTEW 1021
            ACLMDLY+EKMRYKA+N MSRSYRPSLPVPYIAQVLGF TSSG      DVDGLEECTEW
Sbjct: 901  ACLMDLYSEKMRYKALNSMSRSYRPSLPVPYIAQVLGFSTSSGDEVRDKDVDGLEECTEW 960

Query: 1022 LKGHGACLITDNNGEMQLDTKASSTTLYMPEPEDAVAHGDANLAVNDFFTRTSS 1069
            LKGHGACLITDNNGEMQLD KASSTTLYMPEPEDAVAHGDA+LAVNDFFTR SS
Sbjct: 961  LKGHGACLITDNNGEMQLDAKASSTTLYMPEPEDAVAHGDASLAVNDFFTRASS 1005

BLAST of Spg028591 vs. NCBI nr
Match: XP_022967428.1 (SAC3 family protein A isoform X2 [Cucurbita maxima])

HSP 1 Score: 1810.4 bits (4688), Expect = 0.0e+00
Identity = 925/1014 (91.22%), Postives = 954/1014 (94.08%), Query Frame = 0

Query: 62   MNQDGNTETVPPVQPSSLENQYIGDGNQSVATSTYLPLTSAPEAISWANHKVDGSSNENG 121
            MNQ GNTET+ P QPSSLENQ+IGDGNQ   TSTYLPLTSAPEA++WANHKVDGSSNENG
Sbjct: 1    MNQGGNTETLVPAQPSSLENQHIGDGNQPATTSTYLPLTSAPEALAWANHKVDGSSNENG 60

Query: 122  LLPNSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSADPYGY 181
            LLPNST+QYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNS+DPYGY
Sbjct: 61   LLPNSTFQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSSDPYGY 120

Query: 182  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQSLSSFQNTGFYAGSTSYSTTYYNPG 241
            ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQ LSS+QNTGFYAGSTSYSTTYYNPG
Sbjct: 121  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQPLSSYQNTGFYAGSTSYSTTYYNPG 180

Query: 242  DYQTAGGYQTSSYSNQTT-SWNGGNYGNYVPNQYAQYTPDSSGAYSSSSTNANSLQYQQH 301
            DYQTAGGY TSSYSNQTT SWNGGNYGNYVPNQY+QYT DSSGAYSSSSTNANSLQYQQH
Sbjct: 181  DYQTAGGYPTSSYSNQTTSSWNGGNYGNYVPNQYSQYTTDSSGAYSSSSTNANSLQYQQH 240

Query: 302  YKQWADYYSQTEVSCAPGTEKLSTPGTANAGYPVHGSTSYPAPSSQPPPSYTPSWRPESS 361
            YKQWADYYSQTEVSCAPGTEKLS PGTANAGY VHGST+YPAP+SQPPPSYTPSWRPES 
Sbjct: 241  YKQWADYYSQTEVSCAPGTEKLSMPGTANAGYQVHGSTNYPAPNSQPPPSYTPSWRPESG 300

Query: 362  SSELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLDLKTSYDSFQGQQKSA 421
            SSELVSAQPGAVSSGNHDGYWKHG PNSQVHLTNATQPHFEKPLDLKTSYDSFQ QQKSA
Sbjct: 301  SSELVSAQPGAVSSGNHDGYWKHGTPNSQVHLTNATQPHFEKPLDLKTSYDSFQDQQKSA 360

Query: 422  GPQGPNLQYPAHLAPQSYQLPSQSVPSLEARRTKLQIPTNPRIASNLSILKTSKDSSTVD 481
            GPQGPNLQYPAHLAPQSYQLPSQSVP LEARRTKLQIPTNPRIASNLSILKTSKDSST D
Sbjct: 361  GPQGPNLQYPAHLAPQSYQLPSQSVPPLEARRTKLQIPTNPRIASNLSILKTSKDSSTAD 420

Query: 482  AAVQPAYVSVSMPKPNEKELSNDTAEAVLKPGMFPKSLRGYVERALARCKDEKLMTSCQS 541
            AAVQPAYVSV +PKPNEKELSNDTAE+VLKPGMFPKSLRGYVERALARCKDEKLMTSCQS
Sbjct: 421  AAVQPAYVSVLLPKPNEKELSNDTAESVLKPGMFPKSLRGYVERALARCKDEKLMTSCQS 480

Query: 542  VLKEMITKATADGTLYTKDWDIEPLFPLPSADAVNTEYYFSLAKLNNLQGSSPLSSLSKP 601
            VLKE    ATADGTLYTKDWD+EPLFPLPSADAVN++         NL GS+PLSSLSK 
Sbjct: 481  VLKE----ATADGTLYTKDWDVEPLFPLPSADAVNSD---------NLHGSTPLSSLSKS 540

Query: 602  KRSPSRRSKSRWEPLPVEKPAEAHPPYSNGAAAKYDGWANVSERDKKTLSGNSETKNVVN 661
            KRSPSRRSKSRWEPLPVEKPAEA P YSNGAA KY GWANVSERDKK LSGNSETK+V N
Sbjct: 541  KRSPSRRSKSRWEPLPVEKPAEAPPSYSNGAATKYGGWANVSERDKKNLSGNSETKDVSN 600

Query: 662  SRFPLWDQRTVGKISQGPAKKQRVADGSAPDNDGASSDSDKEQSLTAYYSGAMALANSPE 721
            SRF LW+QRTVGKISQGPAKKQRV DGSA DNDGAS DSDKEQSLTAYYSGAMALANSPE
Sbjct: 601  SRFTLWNQRTVGKISQGPAKKQRVLDGSALDNDGASGDSDKEQSLTAYYSGAMALANSPE 660

Query: 722  EKKKRENRSKRFEKGHGHRGENNHFKPKNTGTGNLYTRRASALVLGKNLENGGCRAVEDI 781
            EK+KRE RSKRFEKGHGHRGENNHFKPKN G G+LYTRRASALVLGKNLE+GGCRAVEDI
Sbjct: 661  EKRKRETRSKRFEKGHGHRGENNHFKPKNAGIGSLYTRRASALVLGKNLEDGGCRAVEDI 720

Query: 782  DWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVQMSQKNYLYKCDQLKSI 841
            DWDALTIKGTCQEIEKRYLRLTSAPDP SVRPEEVLEK+LNMVQMSQKNYLYKCDQLKSI
Sbjct: 721  DWDALTIKGTCQEIEKRYLRLTSAPDPCSVRPEEVLEKSLNMVQMSQKNYLYKCDQLKSI 780

Query: 842  RQDLTVQRIRNQLTAKVYEIHARLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYN 901
            RQDLTVQRIRNQLTAKVYE H RLALEVGDLPEYNQCQSQL TLYAEGIEGC+MEFAAYN
Sbjct: 781  RQDLTVQRIRNQLTAKVYETHGRLALEVGDLPEYNQCQSQLTTLYAEGIEGCYMEFAAYN 840

Query: 902  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRSAVTSGNYVKFFRLHKAAPNLN 961
            LLCAILHSNNKRDLLSLMSRLSDQAKKDVAV+HALAVR+AVTSGNYVKFFRL+KAAPNLN
Sbjct: 841  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVSHALAVRAAVTSGNYVKFFRLYKAAPNLN 900

Query: 962  ACLMDLYAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGFFTSSG------DVDGLEECTEW 1021
            ACLMDLY+EKMRYKA+N MSRSYRPSLPVPYIAQVLGF TSSG      DVDGLEECTEW
Sbjct: 901  ACLMDLYSEKMRYKALNSMSRSYRPSLPVPYIAQVLGFSTSSGDEVRDKDVDGLEECTEW 960

Query: 1022 LKGHGACLITDNNGEMQLDTKASSTTLYMPEPEDAVAHGDANLAVNDFFTRTSS 1069
            LKGHGACLITDNNGEMQLD KASSTTLYMPEPEDAVAHGDA+LAVNDFFTR SS
Sbjct: 961  LKGHGACLITDNNGEMQLDAKASSTTLYMPEPEDAVAHGDASLAVNDFFTRASS 1001

BLAST of Spg028591 vs. ExPASy Swiss-Prot
Match: F4IUY8 (SAC3 family protein A OS=Arabidopsis thaliana OX=3702 GN=SAC3A PE=1 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 8.7e-297
Identity = 580/1032 (56.20%), Postives = 708/1032 (68.60%), Query Frame = 0

Query: 62   MNQDGNTETVPPVQPSSLENQYIGDGNQSVATSTYLPLTSAPEAISWANHKVDGSSNENG 121
            MN  GNT+ V P+ P+S+EN+Y  DG+Q+   S     ++  E+  W  H V+  + ENG
Sbjct: 1    MNHGGNTQAVAPMDPNSIENRYGVDGSQTQKYS--YQYSTGSESAPWTGHSVENQAVENG 60

Query: 122  LLPNSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSADPYGY 181
               NS Y + Q   P   NVQ+  N  S   SS+S  T+N  QDY+ Y PY  S+DP+ Y
Sbjct: 61   NYSNSNYYHPQPTGPATGNVQEIPNTVSFTISSTS-GTANVAQDYSGYTPYQTSSDPHNY 120

Query: 182  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQSLSSFQNTGFYAGSTSYSTTYYNPG 241
            +N GY  YY+ YQQQP+ SY QPVGAYQNTGAP Q LSSFQN G YAG+ SYS TYYNP 
Sbjct: 121  SNTGYSNYYSGYQQQPSQSYPQPVGAYQNTGAP-QPLSSFQNPGSYAGTPSYSGTYYNPA 180

Query: 242  DYQTAGGYQ-------------TSSYSNQTTSWNGGNYGNYVPNQYAQYTPDSSGAYSSS 301
            DYQTAGGYQ             +++YSNQT + N GNY +Y  N Y  YTPD++  +SS+
Sbjct: 181  DYQTAGGYQSTNYNNQTAGSYPSTNYSNQTPASNQGNYTDYTSNPYQNYTPDAANTHSST 240

Query: 302  STNANSLQYQQHYKQWADYYSQTEVSCAPGTEKLSTPGTA--NAGYPVHGSTS-YPAPSS 361
                  + YQQ+Y+QW +YYSQTEV CAPGTEKLSTP T+  +  +PV G TS  PA +S
Sbjct: 241  IATTPPVHYQQNYQQWTEYYSQTEVPCAPGTEKLSTPTTSAYSQSFPVPGVTSEMPASNS 300

Query: 362  QPPPSYTPSWRPESSSSELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLD 421
            QP PSY   WRPE+ SS   S QPGA  S ++D YW H AP+ Q H     Q +++ PL+
Sbjct: 301  QPAPSYVQPWRPETDSSHPPSQQPGAAVSTSNDTYWMHQAPSLQAHHPVPPQNNYQSPLE 360

Query: 422  LKTSYDS-FQGQQKSAGPQGPNLQYPAHLAPQSYQLPSQSVPSLEARR-TKLQIPTNPRI 481
             K  Y++ FQG Q++  PQ  N Q   H AP  Y+ P+Q+ P ++++R +K+QIPTNPRI
Sbjct: 361  TKPLYETPFQGHQRATYPQEMNSQSSFHQAPLGYRQPTQTAPLVDSQRVSKVQIPTNPRI 420

Query: 482  ASNL--SILKTSKDSSTVDAAVQPAYVSVSMPKPNEKELSNDTAEAVLKPGMFPKSLRGY 541
            ASNL     K  KDS+   AA  PAYVSVSMPKP       D   A+  PG FPKSLRG+
Sbjct: 421  ASNLPSGFTKMDKDSTAASAAQAPAYVSVSMPKP------KDHTTAMSDPGTFPKSLRGF 480

Query: 542  VERALARCKDEKLMTSCQSVLKEMITKATADGTLYTKDWDIEPLFPLPSADAVNTEYYFS 601
            VERA ARCKD+K   SC+  L++++ KA  D TLYT+DWD EPL  + + +  N+E    
Sbjct: 481  VERAFARCKDDKEKESCEVALRKIVKKAKEDNTLYTRDWDTEPLSTVTTTNVTNSE---- 540

Query: 602  LAKLNNLQGSSPLSSLSKPKRSPSRRSKSRWEPLPVEKPAEAHPPYSNGAAAKYDGWANV 661
                     S+ LSSL    +SP+RR KSRWEPL   KP    P  +  +A K+  W + 
Sbjct: 541  -------SSSAQLSSLQ--NKSPTRRPKSRWEPLVEGKPF-VKPASTFSSAVKFGVWNHQ 600

Query: 662  SERDKKTLSGNSETKNVVNSRFPLWDQRTVGKISQGPAKKQRVADGSAPD-NDGASSDSD 721
            +E +KK+     +       +     Q +  K  Q P K+QR + G+A   +D ASSDSD
Sbjct: 601  NENNKKSSESFQKVDAATGFKPTYSGQNSAKKSFQRPVKRQRFSGGAATAIDDEASSDSD 660

Query: 722  KEQSLTAYYSGAMALANSPEEKKKRENRSKRFEKGHGHRGENNHFKPKNTGTGNLYTRRA 781
            K+  LT YYS AMALA S EEKK+R++RSKRFEK  GH   N+  KPKN   GNL++RRA
Sbjct: 661  KD--LTPYYSSAMALAGSAEEKKRRDSRSKRFEKIQGHSRGNDLTKPKNANVGNLHSRRA 720

Query: 782  SALVLGKNLENGGCRAVEDIDWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKAL 841
            +AL L K  +  G RAVEDIDWDALT+KGTCQEIEKRYLRLTSAPDP++VRPE+VLEKAL
Sbjct: 721  TALRLSKVFDESGSRAVEDIDWDALTVKGTCQEIEKRYLRLTSAPDPATVRPEDVLEKAL 780

Query: 842  NMVQMSQKNYLYKCDQLKSIRQDLTVQRIRNQLTAKVYEIHARLALEVGDLPEYNQCQSQ 901
             MVQ SQKNYL+KCDQLKSIRQDLTVQRI N LTAKVYE HARLALE GDLPEYNQC SQ
Sbjct: 781  IMVQDSQKNYLFKCDQLKSIRQDLTVQRIHNHLTAKVYETHARLALEAGDLPEYNQCLSQ 840

Query: 902  LKTLYAEGIEGCHMEFAAYNLLCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRSA 961
            LKTLYAEG+EGC +EFAAY+LL   LHSNN R+LLS MSRLS++ KKD AV HAL+VR+A
Sbjct: 841  LKTLYAEGVEGCSLEFAAYSLLYITLHSNNNRELLSSMSRLSEEDKKDEAVRHALSVRAA 900

Query: 962  VTSGNYVKFFRLHKAAPNLNACLMDLYAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGFF- 1021
            VTSGNYV FFRL+K APN+N+CLMDLY EKMRYKAVN MSRS RP++PV YI QVLGF  
Sbjct: 901  VTSGNYVMFFRLYKTAPNMNSCLMDLYVEKMRYKAVNFMSRSCRPTIPVSYIVQVLGFTG 960

Query: 1022 -----TSSGDVDGLEECTEWLKGHGACLITDNNGEMQLDTKASSTTLYMPEPEDAVAHGD 1067
                 T   + DG+E+C EWLK HGA +ITD+NG+M LDTKA+ST+L+MPEPEDAVAHGD
Sbjct: 961  AASEGTDEKETDGMEDCLEWLKTHGANIITDSNGDMLLDTKATSTSLFMPEPEDAVAHGD 1006

BLAST of Spg028591 vs. ExPASy Swiss-Prot
Match: Q96PV6 (Leukocyte receptor cluster member 8 OS=Homo sapiens OX=9606 GN=LENG8 PE=1 SV=3)

HSP 1 Score: 230.7 bits (587), Expect = 7.7e-59
Identity = 230/796 (28.89%), Postives = 356/796 (44.72%), Query Frame = 0

Query: 279  PDSSGAYSSSSTNANSLQYQQHYKQW----------ADYYSQTEVSCAPGTEKLSTPGTA 338
            P +S  Y S +  A++LQ QQ+Y QW          + YY  +         +    G+ 
Sbjct: 66   PVASAQYVSQA-EASALQQQQYY-QWYQQYNYAYPYSYYYPMSMYQSYGSPSQYGMAGSY 125

Query: 339  NAGYPVHGST-SYPAPSSQPP-PSYTPSWRPESSSSELVSAQPGAVSSGNHDGYWKHGAP 398
             +  P   S   +    +QPP P    S   ++   +L SAQP   S+  H  +  +  P
Sbjct: 126  GSATPQQPSAPQHQGTLNQPPVPGMDESMSYQAPPQQLPSAQPPQPSNPPHGAHTLNSGP 185

Query: 399  NSQVHLTNATQPHFEKPLDLKTSYDSFQGQQKSAGPQGPNLQYPAHLAPQSYQLPSQSVP 458
              Q     ATQ                Q    +    GP+         +  QL ++  P
Sbjct: 186  --QPGTAPATQ--------------HSQAGPATGQAYGPHTYTEPAKPKKGQQLWNRMKP 245

Query: 459  SLEARRTKLQIPTNPRIASNLSILKTSKDSSTVDAAVQPAYVSVSMPKPNEKELSNDTAE 518
            +      K  I   P   +  S    ++              S   P+PN +++ N +  
Sbjct: 246  APGTGGLKFNIQKRPFAVTTQSFGSNAEGQH-----------SGFGPQPNPEKVQNHSGS 305

Query: 519  AVL-----KPGMFPKSLRGYVERALARCKDEKLMTSCQSVLKEMITKATADGTLYTKDWD 578
            +       KP  +P+ ++ YVER    C+ E+     + +LKE++     DG+ YT DW 
Sbjct: 306  SARGNLSGKPDDWPQDMKEYVERCFTACESEEDKDRTEKLLKEVLQARLQDGSAYTIDWS 365

Query: 579  IEPLFPL---PSADAVNTEYYFSLAKLNNLQGSSPLSSLSKPKRSPSRRSKSRWEPLPVE 638
             EPL  L   P A++   + + + + L+  +G+    S ++   +PS+R           
Sbjct: 366  REPLPGLTREPVAESPKKKRWEAASSLHPPRGA---GSATRGGGAPSQRG---------- 425

Query: 639  KPAEAHPPYSNGAAAKYDGWANVSERDKKTLSGNSETKNVVNSRFPLWDQRTVGKIS--- 698
             P       + G +    G  NV  +D  + S ++++++  +SR P    R     S   
Sbjct: 426  TPGAGGAGRARGNSFTKFGNRNVFMKDNSS-SSSTDSRSRSSSRSPTRHFRRSDSHSDSD 485

Query: 699  -------------QGPAKKQRVADGSAPDNDGASSDSDKEQSLTAYYSGAMALA----NS 758
                         + P  K R   G+  D     +   K   L         +A      
Sbjct: 486  SSYSGNECHPVGRRNPPPKGRGGRGAHMDRGRGRAQRGKRHDLAPTKRSRKKMAALECED 545

Query: 759  PEEKKKRENRSKRFEKGHGHRGENNHFKPKNTGTGNLYTRRASALVLG-KNLENGGCRAV 818
            PE + K++ R+ RF+ GH  R                   R   LVL   +LE+ G    
Sbjct: 546  PERELKKQKRAARFQHGHSRR------------------LRLEPLVLQMSSLESSGA--- 605

Query: 819  EDIDWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVQ---MSQKNYLYKC 878
             D DW  L I GTC +I K YLRLT APDPS+VRP  VL+K+L MV+     +++Y + C
Sbjct: 606  -DPDWQELQIVGTCPDITKHYLRLTCAPDPSTVRPVAVLKKSLCMVKCHWKEKQDYAFAC 665

Query: 879  DQLKSIRQDLTVQRIRNQLTAKVYEIHARLALEVGDLPEYNQCQSQLKTLYAEGIEGCHM 938
            +Q+KSIRQDLTVQ IR + T +VYE HAR+ALE GD  E+NQCQ+QLK+LYAE + G   
Sbjct: 666  EQMKSIRQDLTVQGIRTEFTVEVYETHARIALEKGDHEEFNQCQTQLKSLYAENLPGNVG 725

Query: 939  EFAAYNLLCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRSAVTSGNYVKFFRLHK 998
            EF AY +L  I  + N  D+ + ++ L+ + K D  V HALA+R+A   GNY +FFRL+ 
Sbjct: 726  EFTAYRILYYIF-TKNSGDITTELAYLTRELKADPCVAHALALRTAWALGNYHRFFRLYC 785

Query: 999  AAPNLNACLMDLYAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGFFTSSGDVDGLEECTEW 1031
             AP ++  L+D +A++ R  A+  M +++RP+LPV Y+   L F       +G   C  +
Sbjct: 786  HAPCMSGYLVDKFADRERKVALKAMIKTFRPALPVSYLQAELAF-------EGEAACRAF 788

BLAST of Spg028591 vs. ExPASy Swiss-Prot
Match: Q32NW2 (Leukocyte receptor cluster member 8 homolog OS=Xenopus laevis OX=8355 GN=leng8 PE=2 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 7.2e-57
Identity = 226/806 (28.04%), Postives = 345/806 (42.80%), Query Frame = 0

Query: 289  STNANSLQYQQHYKQWADYYSQTEVSCAPGTEKLSTPGTANAGYPVHGSTSYPAPSSQPP 348
            S    ++  QQ Y QW   YSQ   S              N  Y ++    Y  P     
Sbjct: 70   SQQGEAILQQQQYYQW---YSQYNYSY-----------PYNYYYQMNMYNGYNTPGQYGM 129

Query: 349  PSYTPSWRPESSSSELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLDLKT 408
            P    S   +S   +   AQP     G  D    +    S V L N TQ    + ++   
Sbjct: 130  PGNYQSMSSQSGQHQGNLAQPPV--PGLEDSSMSYSNVQSPVSLCN-TQTASHQQVNHSM 189

Query: 409  SYDSFQGQQKSAGPQGPNLQYPAHLAPQSY----------QLPSQSVPSLEARRTKLQIP 468
            +    QG+         +     + + QS+          QL ++   +  +   K  I 
Sbjct: 190  TKPCIQGRNAGNQSNPHSSSNQPNYSQQSFNEGPKQKKGQQLWNRMKHAPGSGGFKFNIQ 249

Query: 469  TNPRIASNLSILKTS-KDSSTVDAAVQPAYVSVSMPKPNEK---ELSNDTAEAVLKPGMF 528
              P + +N +   +   D+S      Q  ++   + +P ++   E   +      KP  +
Sbjct: 250  KRPLVMANQNFASSEHHDNSAGQQQQQATHMHHPLQQPQQQPTIEKITEHGNNSSKPEDW 309

Query: 529  PKSLRGYVERALARCKDEKLMTSCQSVLKEMITKATADGTLYTKDWDIEPLFPLPSAD-- 588
            P++++ YV+R    C+ E+     + +LKE++     DG+ YT DW  E   PLP  D  
Sbjct: 310  PQAMKEYVQRCFTSCESEEDKDRTEKLLKEVLQARLQDGSAYTIDWSRE---PLPGKDGG 369

Query: 589  ------------AVNTEYYFSLAKLNNLQG---SSPLSSL-------------------S 648
                         + T +  ++    + +G   S+  ++L                   S
Sbjct: 370  KESPKKKRWEQTTLQTSHGSTITITQSPRGGGNSTNAATLRGRGSFIKKFGNRNVFMKES 429

Query: 649  KPKRSPSRRSKSRWEPLPVEKPAEAHPPYSNGAAAKYDGWANVSERDKKTLSGNSETKNV 708
                S   RS+SR    P   P   +    + + + Y G         +T  G       
Sbjct: 430  SSSSSAGSRSRSR---SPSHSPHRRYRRSDSDSDSAYSG--------NETRDGGRRNFQK 489

Query: 709  VNSRFPLWDQRTVGKISQGPAKKQRVADGSAPDNDGASSDSDKEQSLTAYYSGAMALANS 768
               R    D+   G++ +G    Q  +  +   N  A    D                  
Sbjct: 490  ARGRGGHMDRGGRGRLQKGKRSDQAFSKKNRKKNPVAMELED------------------ 549

Query: 769  PEEKKKRENRSKRFEKGHGHRGENNHFKPKNTGTGNLYTRRASALVLGKNLENGGCRAVE 828
            PE++ K+E R+ RF+ GHG         PK          R   LVL   + N    A +
Sbjct: 550  PEKEFKKEKRAARFQHGHG---------PKKL--------RMEPLVL--QINNMDPSAAD 609

Query: 829  DIDWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVQMSQKN---YLYKCD 888
            ++DWD + I G  Q+I K YLRLT APDPS+VRP  VL+K+L MV+   KN   Y++ C+
Sbjct: 610  NLDWDEIKIVGNSQDITKHYLRLTCAPDPSTVRPVPVLKKSLTMVKADFKNKQDYVFACE 669

Query: 889  QLKSIRQDLTVQRIRNQLTAKVYEIHARLALEVGDLPEYNQCQSQLKTLYAEGIEGCHME 948
            Q+KSIRQDLTVQ IR + T +VYE HAR+ALE GD  E+NQCQ+QLK+LYAE + G   E
Sbjct: 670  QMKSIRQDLTVQGIRTEFTVEVYETHARIALEKGDHEEFNQCQAQLKSLYAENLAGNVGE 729

Query: 949  FAAYNLLCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRSAVTSGNYVKFFRLHKA 1008
            F AY +L  I  + N  DL + ++ L+ + K D  V HAL++R A    NY +FF+L++ 
Sbjct: 730  FTAYRILYYIF-TKNSGDLTTELAHLTKELKADACVAHALSLREAWALSNYHRFFKLYRQ 789

Query: 1009 APNLNACLMDLYAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGFFTSSGDVDGLEECTEWL 1042
            AP ++  L+D +AE+ R  A+  M +++RP LPV ++   L F          EEC  +L
Sbjct: 790  APRMSGYLIDKFAERERKAALKAMIKTFRPLLPVSFVQSELAFANE-------EECQSFL 797

BLAST of Spg028591 vs. ExPASy Swiss-Prot
Match: Q8CBY3 (Leukocyte receptor cluster member 8 homolog OS=Mus musculus OX=10090 GN=Leng8 PE=1 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 3.9e-55
Identity = 216/739 (29.23%), Postives = 332/739 (44.93%), Query Frame = 0

Query: 279 PDSSGAYSSSSTNANSLQYQQHYKQW---------ADYYSQTEVSCAPGTEK----LSTP 338
           P +S  Y S +  +   Q QQ Y QW           YY    +  + G+       S+ 
Sbjct: 65  PVASAQYVSQAEASALQQQQQQYYQWYQQYNYAYPYSYYYPMSMYQSYGSPSQYGMASSY 124

Query: 339 GTANAGYPVHGSTSYPAPSSQPP-PSYTPSWRPESSSSELVSAQPGAVSSGNHDGYWKHG 398
           G+A A  P   +  +    +QPP P    S   ++S  +L +AQP   S+  H  +    
Sbjct: 125 GSATAQQP--SAPQHQGTLNQPPVPGMDESMAYQASPQQLPAAQPPQPSNSQHGTH---- 184

Query: 399 APNSQVHLTNATQPHFEKPLDLKTSYDSFQGQQKSAGPQGPNLQYPAHLAPQSYQLPSQS 458
                  L+N  QP          S    Q    +    GP+         +  QL ++ 
Sbjct: 185 ------SLSNGPQP------GTAPSTQHSQAGAPTGQAYGPHSYSEPAKPKKGQQLWTRM 244

Query: 459 VPSLEARRTKLQIPTNPRIASNLSILKTSKDSSTVDAAVQPAYVSVSMPKPNEKELSNDT 518
            P+      K  I   P   ++ S    S+              S   P+PN +   N +
Sbjct: 245 KPAPGTGGLKFNIQKRPFAVTSQSFSSNSEGQH-----------SSFGPQPNSENTQNRS 304

Query: 519 AEAVL-----KPGMFPKSLRGYVERALARCKDEKLMTSCQSVLKEMITKATADGTLYTKD 578
             +       KP  +P+ ++ YVER    C+ E+     + +LKE++     DG+ YT D
Sbjct: 305 GPSGRGNLSGKPDDWPQDMKEYVERCFTACESEEDKDRTEKLLKEVLQARLQDGSAYTID 364

Query: 579 WDIEPLFPL---PSADAVNTEYYFSLAKLNNLQGSSPLS---SLSKPKRSPSRRSKSRWE 638
           W  EPL  L   P A++   + + + + L+  +G+  ++        + +P      R  
Sbjct: 365 WSREPLPGLTREPVAESPKKKRWEAPSSLHPSRGAGSVTRGGGAQSQRGTPGAGGAGRAR 424

Query: 639 PLPVEKPAEAHP-PYSNGAAAKYDGWANVSERDKKTLSGNSETKNVVNSRFPLWDQRTVG 698
                K    +     N +++  D  +  S R        S++ +  +S +   +   VG
Sbjct: 425 GSSFTKFGNRNVFMKDNSSSSSTDSRSRSSSRSPTRHFRRSDSHSDSDSSYSGNECHPVG 484

Query: 699 KISQGPAKKQRVADGSAPDNDGASSDSDKEQSLTAYYSGAMALA----NSPEEKKKRENR 758
           +  + P  K R   G+  D     +   K   L         +A      PE + K++ R
Sbjct: 485 R--RNPPPKGRGGRGAHMDRGRGRAQRGKRHDLAPTKRSRKKMAALECEDPERELKKQKR 544

Query: 759 SKRFEKGHGHRGENNHFKPKNTGTGNLYTRRASALVLG-KNLENGGCRAVEDIDWDALTI 818
           + RF+ GH  R                   R   LVL   NLE+ G     D DW  L I
Sbjct: 545 AARFQHGHSRR------------------LRLEPLVLQMSNLESSGA----DPDWQELQI 604

Query: 819 KGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVQ---MSQKNYLYKCDQLKSIRQDL 878
            GTC +I K YLRLT APDPS+VRP  VL+K+L MV+     +++Y + C+Q+KSIRQDL
Sbjct: 605 VGTCPDITKHYLRLTCAPDPSTVRPVAVLKKSLCMVKSHWKEKQDYAFACEQMKSIRQDL 664

Query: 879 TVQRIRNQLTAKVYEIHARLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYNLLCA 938
           TVQ IR + T +VYE HAR+ALE GD  E+NQCQ+QLK+LYAE + G   EF AY +L  
Sbjct: 665 TVQGIRTEFTVEVYETHARIALEKGDHEEFNQCQTQLKSLYAENLAGNVGEFTAYRILYY 724

Query: 939 ILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRSAVTSGNYVKFFRLHKAAPNLNACLM 984
           I  + N  D+ + ++ L+ + K D  V HALA+R+A   GNY +FFRL+  AP ++  L+
Sbjct: 725 IF-TKNSGDITTELAYLTREMKADPCVAHALALRAAWALGNYHRFFRLYCHAPCMSGYLV 749

BLAST of Spg028591 vs. ExPASy Swiss-Prot
Match: A4QNR8 (Leukocyte receptor cluster member 8 homolog OS=Danio rerio OX=7955 GN=leng8 PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 5.0e-50
Identity = 256/915 (27.98%), Postives = 377/915 (41.20%), Query Frame = 0

Query: 182  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQSLSSFQNTGFYAGSTSYST--TYYN 241
            ANA  Q    N+    N S   PV          Q+L+S   +     ST  ST  T   
Sbjct: 3    ANAAQQAPTQNWPYGNNSSGEGPVHENPEWEKARQALASISKS---QNSTKPSTNQTTQQ 62

Query: 242  PGDYQTA----GGYQTSSYSNQTTSWNGGNYGNYVPNQYAQYTP-------------DSS 301
             G YQ A       Q   Y  Q    N   Y  Y P  Y  Y P                
Sbjct: 63   AGQYQAAVTDSSAMQQQQYYQQWYQQNQQQYAGY-PYPYNYYYPMPPYGGPYPPGQYGVP 122

Query: 302  GAYSSSST---NANSLQYQQHYKQWADYYSQTEVSCAP------GTEKLSTPGTANAGYP 361
            G Y + +T    A S+   +   Q ++Y  Q      P       T     P       P
Sbjct: 123  GGYQTPTTPTGQAPSMPTME--DQSSNYPPQPPPPATPQPPPQSPTSSDQPPPPPPPPIP 182

Query: 362  VHGSTSYPAPSSQPP--PSYTPSWRPESSSSELVSAQPGAVSSGNHDGYWKHGAPNSQVH 421
               +  YP P SQ P  P+    + P  S+  +    PG +      GY  +  P  Q H
Sbjct: 183  PQPNAQYPRPPSQTPYSPNGVLPYSPTDSNPMMRGYNPGQI----RPGYQGYQTPVYQPH 242

Query: 422  LTNATQPHFEKPLDLKTSYDSFQGQQKSAGPQGPNLQYPAHLAPQSYQLPSQSVPSLEAR 481
               A  P                G       + PN         Q +Q   Q+ P   A 
Sbjct: 243  QQQAPAP-------------GLYGGAGRVDAKKPNNSNMNKGGQQLWQRMKQA-PGTGA- 302

Query: 482  RTKLQIPTNPRIASNLSILKTSKDSSTV-DAAVQPAYVSVSMPKPNEKELSNDTAEAVLK 541
              K  IP  P + SN +     +        A  P+  S S         S  T  A  +
Sbjct: 303  -VKFNIPKRPYVMSNQNFPPGEQGPGLAPPTATNPSSGSTSATS------SASTGGAQTR 362

Query: 542  PGMFPKSLRGYVERALARCKDEKLMTSCQSVLKEMITKATADGTLYTKDWDIEPLFPLPS 601
            P  +P++++ YV+R    C+ E+     + +LK+++     DG+ YT DW+ EPL  L  
Sbjct: 363  PQDWPQAMKEYVQRCFTACESEEDKDRTEKMLKDLLQSRLQDGSAYTIDWNREPLPDLKP 422

Query: 602  ADAVNTEYYFSLAKLNNL--------QGSSPLSSLSK--PKRSP------SRRSKSRWEP 661
              +   E    L++ N++        +G + L +      +RSP      S RS SR  P
Sbjct: 423  KQS-RWEVMPQLSQENSIGRGGATRGRGGARLGNYRNVFSQRSPSSSSSGSSRSSSR-SP 482

Query: 662  LPVEKPAEAHPPYSNGAAAKYDGWAN----VSERDKKTLSGNSETKNVVNSRFPLWDQ-R 721
             P  +  +    + + + ++ DG  +    +S   ++   G          R    ++ R
Sbjct: 483  SPHSRHRDRSRHHRSDSGSQSDGSMSSDLRLSLTRRQQKGGRGRGAERERGRGAAGERGR 542

Query: 722  TVGKISQGPAKKQRVADGSAPDNDGASSDSDKEQSLTAYYSGAMALANSPEEKKKRENRS 781
              GK ++G    + +A G      G ++  D                + P  + K+++R+
Sbjct: 543  GRGKANRGRRNMEDLASGVGKKRKGGNAGLD---------------FHDPNREAKKQSRA 602

Query: 782  KRFEKGHGHRGENNHFKPKNTGTGNLYTRRASALVLGKNLENGGCRAVEDIDWDALTIKG 841
             RF           H K            R   LVL  N+ +      E + WD   I G
Sbjct: 603  ARF-----------HTK-----------LRTEPLVLNINVFDLPNGTQEGLSWDDCPIVG 662

Query: 842  TCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVQ---MSQKNYLYKCDQLKSIRQDLTV 901
            TCQ+I K YLRLT APDPS+VRP  VL K+L  V+    S ++Y+Y C+Q+KSIRQDLTV
Sbjct: 663  TCQDITKNYLRLTCAPDPSTVRPVPVLRKSLIAVKAHWKSNQDYVYACEQMKSIRQDLTV 722

Query: 902  QRIRNQLTAKVYEIHARLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYNLLCAIL 961
            Q +R   T +VYE HAR+ALE GD  E+NQCQ+QLK LY +       EF AY L+  I 
Sbjct: 723  QGVRTDFTVEVYETHARIALEKGDHEEFNQCQTQLKALYKDCPSDNVGEFTAYRLIYYIF 782

Query: 962  HSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRSAVTSGNYVKFFRLHKAAPNLNACLMDL 1021
             + N  DL + +  L+ + + D  V HAL +R+A   GN+ +FFRL++ AP + A L+D 
Sbjct: 783  -TKNSGDLTTELVYLTTELRADPCVAHALELRTAWALGNFHRFFRLYQKAPRMAAYLIDK 836

Query: 1022 YAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGFFTSSGDVDGLEECTEWLKGHGACLITDN 1042
            + E+ R  A+  + +S+RPS+ V Y+   L F         L+ C  +L G G      +
Sbjct: 843  FVERERNIALRAILKSFRPSVSVEYVQSSLAF-------PDLDTCLAFLTGLGISFTPSD 836

BLAST of Spg028591 vs. ExPASy TrEMBL
Match: A0A6J1GWE5 (SAC3 family protein A-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458194 PE=4 SV=1)

HSP 1 Score: 1829.3 bits (4737), Expect = 0.0e+00
Identity = 933/1014 (92.01%), Postives = 960/1014 (94.67%), Query Frame = 0

Query: 62   MNQDGNTETVPPVQPSSLENQYIGDGNQSVATSTYLPLTSAPEAISWANHKVDGSSNENG 121
            MNQ GNTET+ P QPSSLENQ+IGDGNQS  TSTYLPLTSAPEAI+WANHKVDGSSNENG
Sbjct: 1    MNQGGNTETLVPAQPSSLENQHIGDGNQSATTSTYLPLTSAPEAIAWANHKVDGSSNENG 60

Query: 122  LLPNSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSADPYGY 181
            LLPNST+QYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNS+DPYGY
Sbjct: 61   LLPNSTFQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSSDPYGY 120

Query: 182  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQSLSSFQNTGFYAGSTSYSTTYYNPG 241
            ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQ LSS+QNTGFYAGSTSYSTTYYNPG
Sbjct: 121  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQPLSSYQNTGFYAGSTSYSTTYYNPG 180

Query: 242  DYQTAGGYQTSSYSNQTT-SWNGGNYGNYVPNQYAQYTPDSSGAYSSSSTNANSLQYQQH 301
            DYQTAGGY TSSYSNQTT SWNGGNYGNYVPNQY+QYT DSSGAYSSSSTNANSLQYQQH
Sbjct: 181  DYQTAGGYPTSSYSNQTTSSWNGGNYGNYVPNQYSQYTTDSSGAYSSSSTNANSLQYQQH 240

Query: 302  YKQWADYYSQTEVSCAPGTEKLSTPGTANAGYPVHGSTSYPAPSSQPPPSYTPSWRPESS 361
            YKQWADYYSQTEVSCAPGTEKLS PGTANAGY VHGST+YPAP+SQPPPSYTPSWRPES 
Sbjct: 241  YKQWADYYSQTEVSCAPGTEKLSAPGTANAGYQVHGSTNYPAPNSQPPPSYTPSWRPESG 300

Query: 362  SSELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLDLKTSYDSFQGQQKSA 421
            SSELVSAQPGAVSSGNHDGYWKHG PNSQVHLTNATQPHFEKPLDLKTSYDSFQ QQKSA
Sbjct: 301  SSELVSAQPGAVSSGNHDGYWKHGTPNSQVHLTNATQPHFEKPLDLKTSYDSFQDQQKSA 360

Query: 422  GPQGPNLQYPAHLAPQSYQLPSQSVPSLEARRTKLQIPTNPRIASNLSILKTSKDSSTVD 481
            GPQGPNLQYPAHLAPQSYQLPSQSVP LEARRTKLQIPTNPRIASNLSILKTSKD ST D
Sbjct: 361  GPQGPNLQYPAHLAPQSYQLPSQSVPPLEARRTKLQIPTNPRIASNLSILKTSKDISTAD 420

Query: 482  AAVQPAYVSVSMPKPNEKELSNDTAEAVLKPGMFPKSLRGYVERALARCKDEKLMTSCQS 541
            AAVQPAYVSV +PKPNEKELSNDTAE+VLKPGMFPKSLRGYVERALARCKDEKLMTSCQS
Sbjct: 421  AAVQPAYVSVLLPKPNEKELSNDTAESVLKPGMFPKSLRGYVERALARCKDEKLMTSCQS 480

Query: 542  VLKEMITKATADGTLYTKDWDIEPLFPLPSADAVNTEYYFSLAKLNNLQGSSPLSSLSKP 601
            VLKEMITKATADGTLYTKDWD+EPLFPLPSADAVN++         NL GS+PLSSLSK 
Sbjct: 481  VLKEMITKATADGTLYTKDWDVEPLFPLPSADAVNSD---------NLHGSTPLSSLSKS 540

Query: 602  KRSPSRRSKSRWEPLPVEKPAEAHPPYSNGAAAKYDGWANVSERDKKTLSGNSETKNVVN 661
            KRSPSRRSKSRWEPLPVEKPAEA PPYSNGAA KY GWANVSERDKK LSGNSETK+V N
Sbjct: 541  KRSPSRRSKSRWEPLPVEKPAEA-PPYSNGAATKYGGWANVSERDKKNLSGNSETKDVSN 600

Query: 662  SRFPLWDQRTVGKISQGPAKKQRVADGSAPDNDGASSDSDKEQSLTAYYSGAMALANSPE 721
            SRF LW+QRTVGKISQGPAKKQRV DGSA DNDGAS DSDKEQSLTAYYSGAMALANSPE
Sbjct: 601  SRFTLWNQRTVGKISQGPAKKQRVLDGSALDNDGASGDSDKEQSLTAYYSGAMALANSPE 660

Query: 722  EKKKRENRSKRFEKGHGHRGENNHFKPKNTGTGNLYTRRASALVLGKNLENGGCRAVEDI 781
            EK+KRE RSKRFEKGHGHRGENNHFKPKN G G+LYTRRASALVLGKNLE+GGCRAVEDI
Sbjct: 661  EKRKRETRSKRFEKGHGHRGENNHFKPKNAGIGSLYTRRASALVLGKNLEDGGCRAVEDI 720

Query: 782  DWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVQMSQKNYLYKCDQLKSI 841
            DWDALTIKGTCQEIEKRYLRLTSAPDP SVRPEEVLEK+LNMVQMSQKNYLYKCDQLKSI
Sbjct: 721  DWDALTIKGTCQEIEKRYLRLTSAPDPCSVRPEEVLEKSLNMVQMSQKNYLYKCDQLKSI 780

Query: 842  RQDLTVQRIRNQLTAKVYEIHARLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYN 901
            RQDLTVQRIRNQLTAKVYE H RLALEVGDLPEYNQCQSQL TLYAEGIEGC+MEFAAYN
Sbjct: 781  RQDLTVQRIRNQLTAKVYETHGRLALEVGDLPEYNQCQSQLTTLYAEGIEGCYMEFAAYN 840

Query: 902  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRSAVTSGNYVKFFRLHKAAPNLN 961
            LLCAILHSNNKRDLLSLMSRLSDQAKKDVAV+HALAVR+AVTSGNYVKFFRL+KAAPNLN
Sbjct: 841  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVSHALAVRAAVTSGNYVKFFRLYKAAPNLN 900

Query: 962  ACLMDLYAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGFFTSSG------DVDGLEECTEW 1021
            ACLMDLY+EKMRYKA+NCMSRSYRPSLPVPYIAQVLGF TSSG      DVDGLEECTEW
Sbjct: 901  ACLMDLYSEKMRYKALNCMSRSYRPSLPVPYIAQVLGFSTSSGDEVRDKDVDGLEECTEW 960

Query: 1022 LKGHGACLITDNNGEMQLDTKASSTTLYMPEPEDAVAHGDANLAVNDFFTRTSS 1069
            LKGHGACLITDNNGEMQLD KASSTTLYMPEPEDAVAHGDANLAVNDFFTR SS
Sbjct: 961  LKGHGACLITDNNGEMQLDAKASSTTLYMPEPEDAVAHGDANLAVNDFFTRASS 1004

BLAST of Spg028591 vs. ExPASy TrEMBL
Match: A0A6J1HRS7 (SAC3 family protein A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466883 PE=4 SV=1)

HSP 1 Score: 1823.5 bits (4722), Expect = 0.0e+00
Identity = 929/1014 (91.62%), Postives = 958/1014 (94.48%), Query Frame = 0

Query: 62   MNQDGNTETVPPVQPSSLENQYIGDGNQSVATSTYLPLTSAPEAISWANHKVDGSSNENG 121
            MNQ GNTET+ P QPSSLENQ+IGDGNQ   TSTYLPLTSAPEA++WANHKVDGSSNENG
Sbjct: 1    MNQGGNTETLVPAQPSSLENQHIGDGNQPATTSTYLPLTSAPEALAWANHKVDGSSNENG 60

Query: 122  LLPNSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSADPYGY 181
            LLPNST+QYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNS+DPYGY
Sbjct: 61   LLPNSTFQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSSDPYGY 120

Query: 182  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQSLSSFQNTGFYAGSTSYSTTYYNPG 241
            ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQ LSS+QNTGFYAGSTSYSTTYYNPG
Sbjct: 121  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQPLSSYQNTGFYAGSTSYSTTYYNPG 180

Query: 242  DYQTAGGYQTSSYSNQTT-SWNGGNYGNYVPNQYAQYTPDSSGAYSSSSTNANSLQYQQH 301
            DYQTAGGY TSSYSNQTT SWNGGNYGNYVPNQY+QYT DSSGAYSSSSTNANSLQYQQH
Sbjct: 181  DYQTAGGYPTSSYSNQTTSSWNGGNYGNYVPNQYSQYTTDSSGAYSSSSTNANSLQYQQH 240

Query: 302  YKQWADYYSQTEVSCAPGTEKLSTPGTANAGYPVHGSTSYPAPSSQPPPSYTPSWRPESS 361
            YKQWADYYSQTEVSCAPGTEKLS PGTANAGY VHGST+YPAP+SQPPPSYTPSWRPES 
Sbjct: 241  YKQWADYYSQTEVSCAPGTEKLSMPGTANAGYQVHGSTNYPAPNSQPPPSYTPSWRPESG 300

Query: 362  SSELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLDLKTSYDSFQGQQKSA 421
            SSELVSAQPGAVSSGNHDGYWKHG PNSQVHLTNATQPHFEKPLDLKTSYDSFQ QQKSA
Sbjct: 301  SSELVSAQPGAVSSGNHDGYWKHGTPNSQVHLTNATQPHFEKPLDLKTSYDSFQDQQKSA 360

Query: 422  GPQGPNLQYPAHLAPQSYQLPSQSVPSLEARRTKLQIPTNPRIASNLSILKTSKDSSTVD 481
            GPQGPNLQYPAHLAPQSYQLPSQSVP LEARRTKLQIPTNPRIASNLSILKTSKDSST D
Sbjct: 361  GPQGPNLQYPAHLAPQSYQLPSQSVPPLEARRTKLQIPTNPRIASNLSILKTSKDSSTAD 420

Query: 482  AAVQPAYVSVSMPKPNEKELSNDTAEAVLKPGMFPKSLRGYVERALARCKDEKLMTSCQS 541
            AAVQPAYVSV +PKPNEKELSNDTAE+VLKPGMFPKSLRGYVERALARCKDEKLMTSCQS
Sbjct: 421  AAVQPAYVSVLLPKPNEKELSNDTAESVLKPGMFPKSLRGYVERALARCKDEKLMTSCQS 480

Query: 542  VLKEMITKATADGTLYTKDWDIEPLFPLPSADAVNTEYYFSLAKLNNLQGSSPLSSLSKP 601
            VLKEMITKATADGTLYTKDWD+EPLFPLPSADAVN++         NL GS+PLSSLSK 
Sbjct: 481  VLKEMITKATADGTLYTKDWDVEPLFPLPSADAVNSD---------NLHGSTPLSSLSKS 540

Query: 602  KRSPSRRSKSRWEPLPVEKPAEAHPPYSNGAAAKYDGWANVSERDKKTLSGNSETKNVVN 661
            KRSPSRRSKSRWEPLPVEKPAEA P YSNGAA KY GWANVSERDKK LSGNSETK+V N
Sbjct: 541  KRSPSRRSKSRWEPLPVEKPAEAPPSYSNGAATKYGGWANVSERDKKNLSGNSETKDVSN 600

Query: 662  SRFPLWDQRTVGKISQGPAKKQRVADGSAPDNDGASSDSDKEQSLTAYYSGAMALANSPE 721
            SRF LW+QRTVGKISQGPAKKQRV DGSA DNDGAS DSDKEQSLTAYYSGAMALANSPE
Sbjct: 601  SRFTLWNQRTVGKISQGPAKKQRVLDGSALDNDGASGDSDKEQSLTAYYSGAMALANSPE 660

Query: 722  EKKKRENRSKRFEKGHGHRGENNHFKPKNTGTGNLYTRRASALVLGKNLENGGCRAVEDI 781
            EK+KRE RSKRFEKGHGHRGENNHFKPKN G G+LYTRRASALVLGKNLE+GGCRAVEDI
Sbjct: 661  EKRKRETRSKRFEKGHGHRGENNHFKPKNAGIGSLYTRRASALVLGKNLEDGGCRAVEDI 720

Query: 782  DWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVQMSQKNYLYKCDQLKSI 841
            DWDALTIKGTCQEIEKRYLRLTSAPDP SVRPEEVLEK+LNMVQMSQKNYLYKCDQLKSI
Sbjct: 721  DWDALTIKGTCQEIEKRYLRLTSAPDPCSVRPEEVLEKSLNMVQMSQKNYLYKCDQLKSI 780

Query: 842  RQDLTVQRIRNQLTAKVYEIHARLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYN 901
            RQDLTVQRIRNQLTAKVYE H RLALEVGDLPEYNQCQSQL TLYAEGIEGC+MEFAAYN
Sbjct: 781  RQDLTVQRIRNQLTAKVYETHGRLALEVGDLPEYNQCQSQLTTLYAEGIEGCYMEFAAYN 840

Query: 902  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRSAVTSGNYVKFFRLHKAAPNLN 961
            LLCAILHSNNKRDLLSLMSRLSDQAKKDVAV+HALAVR+AVTSGNYVKFFRL+KAAPNLN
Sbjct: 841  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVSHALAVRAAVTSGNYVKFFRLYKAAPNLN 900

Query: 962  ACLMDLYAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGFFTSSG------DVDGLEECTEW 1021
            ACLMDLY+EKMRYKA+N MSRSYRPSLPVPYIAQVLGF TSSG      DVDGLEECTEW
Sbjct: 901  ACLMDLYSEKMRYKALNSMSRSYRPSLPVPYIAQVLGFSTSSGDEVRDKDVDGLEECTEW 960

Query: 1022 LKGHGACLITDNNGEMQLDTKASSTTLYMPEPEDAVAHGDANLAVNDFFTRTSS 1069
            LKGHGACLITDNNGEMQLD KASSTTLYMPEPEDAVAHGDA+LAVNDFFTR SS
Sbjct: 961  LKGHGACLITDNNGEMQLDAKASSTTLYMPEPEDAVAHGDASLAVNDFFTRASS 1005

BLAST of Spg028591 vs. ExPASy TrEMBL
Match: A0A6J1HV23 (SAC3 family protein A isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466883 PE=4 SV=1)

HSP 1 Score: 1810.4 bits (4688), Expect = 0.0e+00
Identity = 925/1014 (91.22%), Postives = 954/1014 (94.08%), Query Frame = 0

Query: 62   MNQDGNTETVPPVQPSSLENQYIGDGNQSVATSTYLPLTSAPEAISWANHKVDGSSNENG 121
            MNQ GNTET+ P QPSSLENQ+IGDGNQ   TSTYLPLTSAPEA++WANHKVDGSSNENG
Sbjct: 1    MNQGGNTETLVPAQPSSLENQHIGDGNQPATTSTYLPLTSAPEALAWANHKVDGSSNENG 60

Query: 122  LLPNSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSADPYGY 181
            LLPNST+QYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNS+DPYGY
Sbjct: 61   LLPNSTFQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSSDPYGY 120

Query: 182  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQSLSSFQNTGFYAGSTSYSTTYYNPG 241
            ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQ LSS+QNTGFYAGSTSYSTTYYNPG
Sbjct: 121  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQPLSSYQNTGFYAGSTSYSTTYYNPG 180

Query: 242  DYQTAGGYQTSSYSNQTT-SWNGGNYGNYVPNQYAQYTPDSSGAYSSSSTNANSLQYQQH 301
            DYQTAGGY TSSYSNQTT SWNGGNYGNYVPNQY+QYT DSSGAYSSSSTNANSLQYQQH
Sbjct: 181  DYQTAGGYPTSSYSNQTTSSWNGGNYGNYVPNQYSQYTTDSSGAYSSSSTNANSLQYQQH 240

Query: 302  YKQWADYYSQTEVSCAPGTEKLSTPGTANAGYPVHGSTSYPAPSSQPPPSYTPSWRPESS 361
            YKQWADYYSQTEVSCAPGTEKLS PGTANAGY VHGST+YPAP+SQPPPSYTPSWRPES 
Sbjct: 241  YKQWADYYSQTEVSCAPGTEKLSMPGTANAGYQVHGSTNYPAPNSQPPPSYTPSWRPESG 300

Query: 362  SSELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLDLKTSYDSFQGQQKSA 421
            SSELVSAQPGAVSSGNHDGYWKHG PNSQVHLTNATQPHFEKPLDLKTSYDSFQ QQKSA
Sbjct: 301  SSELVSAQPGAVSSGNHDGYWKHGTPNSQVHLTNATQPHFEKPLDLKTSYDSFQDQQKSA 360

Query: 422  GPQGPNLQYPAHLAPQSYQLPSQSVPSLEARRTKLQIPTNPRIASNLSILKTSKDSSTVD 481
            GPQGPNLQYPAHLAPQSYQLPSQSVP LEARRTKLQIPTNPRIASNLSILKTSKDSST D
Sbjct: 361  GPQGPNLQYPAHLAPQSYQLPSQSVPPLEARRTKLQIPTNPRIASNLSILKTSKDSSTAD 420

Query: 482  AAVQPAYVSVSMPKPNEKELSNDTAEAVLKPGMFPKSLRGYVERALARCKDEKLMTSCQS 541
            AAVQPAYVSV +PKPNEKELSNDTAE+VLKPGMFPKSLRGYVERALARCKDEKLMTSCQS
Sbjct: 421  AAVQPAYVSVLLPKPNEKELSNDTAESVLKPGMFPKSLRGYVERALARCKDEKLMTSCQS 480

Query: 542  VLKEMITKATADGTLYTKDWDIEPLFPLPSADAVNTEYYFSLAKLNNLQGSSPLSSLSKP 601
            VLKE    ATADGTLYTKDWD+EPLFPLPSADAVN++         NL GS+PLSSLSK 
Sbjct: 481  VLKE----ATADGTLYTKDWDVEPLFPLPSADAVNSD---------NLHGSTPLSSLSKS 540

Query: 602  KRSPSRRSKSRWEPLPVEKPAEAHPPYSNGAAAKYDGWANVSERDKKTLSGNSETKNVVN 661
            KRSPSRRSKSRWEPLPVEKPAEA P YSNGAA KY GWANVSERDKK LSGNSETK+V N
Sbjct: 541  KRSPSRRSKSRWEPLPVEKPAEAPPSYSNGAATKYGGWANVSERDKKNLSGNSETKDVSN 600

Query: 662  SRFPLWDQRTVGKISQGPAKKQRVADGSAPDNDGASSDSDKEQSLTAYYSGAMALANSPE 721
            SRF LW+QRTVGKISQGPAKKQRV DGSA DNDGAS DSDKEQSLTAYYSGAMALANSPE
Sbjct: 601  SRFTLWNQRTVGKISQGPAKKQRVLDGSALDNDGASGDSDKEQSLTAYYSGAMALANSPE 660

Query: 722  EKKKRENRSKRFEKGHGHRGENNHFKPKNTGTGNLYTRRASALVLGKNLENGGCRAVEDI 781
            EK+KRE RSKRFEKGHGHRGENNHFKPKN G G+LYTRRASALVLGKNLE+GGCRAVEDI
Sbjct: 661  EKRKRETRSKRFEKGHGHRGENNHFKPKNAGIGSLYTRRASALVLGKNLEDGGCRAVEDI 720

Query: 782  DWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVQMSQKNYLYKCDQLKSI 841
            DWDALTIKGTCQEIEKRYLRLTSAPDP SVRPEEVLEK+LNMVQMSQKNYLYKCDQLKSI
Sbjct: 721  DWDALTIKGTCQEIEKRYLRLTSAPDPCSVRPEEVLEKSLNMVQMSQKNYLYKCDQLKSI 780

Query: 842  RQDLTVQRIRNQLTAKVYEIHARLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYN 901
            RQDLTVQRIRNQLTAKVYE H RLALEVGDLPEYNQCQSQL TLYAEGIEGC+MEFAAYN
Sbjct: 781  RQDLTVQRIRNQLTAKVYETHGRLALEVGDLPEYNQCQSQLTTLYAEGIEGCYMEFAAYN 840

Query: 902  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRSAVTSGNYVKFFRLHKAAPNLN 961
            LLCAILHSNNKRDLLSLMSRLSDQAKKDVAV+HALAVR+AVTSGNYVKFFRL+KAAPNLN
Sbjct: 841  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVSHALAVRAAVTSGNYVKFFRLYKAAPNLN 900

Query: 962  ACLMDLYAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGFFTSSG------DVDGLEECTEW 1021
            ACLMDLY+EKMRYKA+N MSRSYRPSLPVPYIAQVLGF TSSG      DVDGLEECTEW
Sbjct: 901  ACLMDLYSEKMRYKALNSMSRSYRPSLPVPYIAQVLGFSTSSGDEVRDKDVDGLEECTEW 960

Query: 1022 LKGHGACLITDNNGEMQLDTKASSTTLYMPEPEDAVAHGDANLAVNDFFTRTSS 1069
            LKGHGACLITDNNGEMQLD KASSTTLYMPEPEDAVAHGDA+LAVNDFFTR SS
Sbjct: 961  LKGHGACLITDNNGEMQLDAKASSTTLYMPEPEDAVAHGDASLAVNDFFTRASS 1001

BLAST of Spg028591 vs. ExPASy TrEMBL
Match: A0A0A0KQ55 (PCI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G177120 PE=4 SV=1)

HSP 1 Score: 1802.7 bits (4668), Expect = 0.0e+00
Identity = 922/1014 (90.93%), Postives = 951/1014 (93.79%), Query Frame = 0

Query: 62   MNQDGNTETVPPVQPSSLENQYIGDGNQSVATSTYLPLTSAPEAISWANHKVDGSSNENG 121
            MNQ GNTET  P QPSSLENQ+IGDGNQSVA STYLPL SAPEAI+WANHKVDGSSNE+G
Sbjct: 1    MNQGGNTETFVPAQPSSLENQHIGDGNQSVAASTYLPLASAPEAITWANHKVDGSSNESG 60

Query: 122  LLPNSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSADPYGY 181
            LL NSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYA YSNS DPYGY
Sbjct: 61   LLSNSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAQYSNSTDPYGY 120

Query: 182  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQSLSSFQNTGFYAGSTSYSTTYYNPG 241
            ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQ LSS+QNTGFYAGSTSYSTTYYNPG
Sbjct: 121  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQPLSSYQNTGFYAGSTSYSTTYYNPG 180

Query: 242  DYQTAGGYQTSSYSNQTTSWNGGNYGNYVPNQYAQYTPDSSGAYSSSSTNANSLQYQQHY 301
            DYQTAGGY TSSYSNQTTSWNGGNYGNYVPNQYAQYTPDSSGAYSS+STN +SLQYQQ  
Sbjct: 181  DYQTAGGYPTSSYSNQTTSWNGGNYGNYVPNQYAQYTPDSSGAYSSTSTNESSLQYQQQC 240

Query: 302  KQWADYYSQTEVSCAPGTEKLSTPGTANAGYPVHGSTSYPAPSSQ-PPPSYTPSWRPESS 361
            KQWADYYSQTEVSCAPGTEKLSTP  ANAGYP HGST+YPAP SQ PPPSYTPSWRPES 
Sbjct: 241  KQWADYYSQTEVSCAPGTEKLSTPSIANAGYPAHGSTNYPAPHSQPPPPSYTPSWRPESG 300

Query: 362  SSELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLDLKTSYDSFQGQQKSA 421
            SSELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLDLK SYDSFQ QQKSA
Sbjct: 301  SSELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLDLKNSYDSFQDQQKSA 360

Query: 422  GPQGPNLQYPAHLAPQSYQLPSQSVPSLEARRTKLQIPTNPRIASNLSILKTSKDSSTVD 481
            GPQGPNLQYPAHLAPQSYQLPSQSV  +EARRTKLQIPTNPRIASNLSILKTSKDSST D
Sbjct: 361  GPQGPNLQYPAHLAPQSYQLPSQSVSPVEARRTKLQIPTNPRIASNLSILKTSKDSSTAD 420

Query: 482  AAVQPAYVSVSMPKPNEKELSNDTAEAVLKPGMFPKSLRGYVERALARCKDEKLMTSCQS 541
            A VQPAYVSVS+PKPNEKELSNDT E+VLKPGMFPKSLRGYVERA+ARCKDEKLMTSCQS
Sbjct: 421  APVQPAYVSVSLPKPNEKELSNDT-ESVLKPGMFPKSLRGYVERAMARCKDEKLMTSCQS 480

Query: 542  VLKEMITKATADGTLYTKDWDIEPLFPLPSADAVNTEYYFSLAKLNNLQGSSPLSSLSKP 601
            VLKEMITKATADGTLYTKDWD+EPLFPLPSADAVNT+         NLQ  +P+SSLSK 
Sbjct: 481  VLKEMITKATADGTLYTKDWDVEPLFPLPSADAVNTD---------NLQVPTPISSLSKS 540

Query: 602  KRSPSRRSKSRWEPLPVEKPAEAHPPYSNGAAAKYDGWANVSERDKKTLSGNSETKNVVN 661
            KRSPSRRSKSRWEPLPVEKPAEA PP+SNGAAAKY GWANVSER+KKTLSGNSETK+  N
Sbjct: 541  KRSPSRRSKSRWEPLPVEKPAEAPPPHSNGAAAKYGGWANVSEREKKTLSGNSETKDASN 600

Query: 662  SRFPLWDQRTVGKISQGPAKKQRVADGSAPDNDGASSDSDKEQSLTAYYSGAMALANSPE 721
            SRFPLWDQRTVGKISQGP+KKQRVADGS PDNDG SSDSDKEQSLTAYYSGAMALANSPE
Sbjct: 601  SRFPLWDQRTVGKISQGPSKKQRVADGSPPDNDGPSSDSDKEQSLTAYYSGAMALANSPE 660

Query: 722  EKKKRENRSKRFEKGHGHRGENNHFKPKNTGTGNLYTRRASALVLGKNLENGGCRAVEDI 781
            EKKKRENRSKRF+KGHGHRGENNHFK KN G G+LYTRRASALV+GKNLENGG RAVEDI
Sbjct: 661  EKKKRENRSKRFDKGHGHRGENNHFKSKNAGIGSLYTRRASALVIGKNLENGGGRAVEDI 720

Query: 782  DWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVQMSQKNYLYKCDQLKSI 841
            DWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMV+ SQKNYLYKCDQLKSI
Sbjct: 721  DWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVKTSQKNYLYKCDQLKSI 780

Query: 842  RQDLTVQRIRNQLTAKVYEIHARLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYN 901
            RQDLTVQRIRNQLTAKVYE H RLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYN
Sbjct: 781  RQDLTVQRIRNQLTAKVYETHGRLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYN 840

Query: 902  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRSAVTSGNYVKFFRLHKAAPNLN 961
            LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVR+AVTS N+VKFFRL+KAAPNLN
Sbjct: 841  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRAAVTSENFVKFFRLYKAAPNLN 900

Query: 962  ACLMDLYAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGFFTSSG------DVDGLEECTEW 1021
            ACLMDLYAEKMRYKA+NCMSRSYRPSLPVPYIAQVLGF TSSG      DVDGLEEC EW
Sbjct: 901  ACLMDLYAEKMRYKAINCMSRSYRPSLPVPYIAQVLGFSTSSGDEVKDKDVDGLEECMEW 960

Query: 1022 LKGHGACLITDNNGEMQLDTKASSTTLYMPEPEDAVAHGDANLAVNDFFTRTSS 1069
            LK HGACLITD+NGEMQLD KASSTTLYMPEP+DAVAHGDANLAVNDFFTRTSS
Sbjct: 961  LKAHGACLITDSNGEMQLDAKASSTTLYMPEPDDAVAHGDANLAVNDFFTRTSS 1004

BLAST of Spg028591 vs. ExPASy TrEMBL
Match: A0A5A7SST2 (SAC3 family protein A isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00030 PE=4 SV=1)

HSP 1 Score: 1795.8 bits (4650), Expect = 0.0e+00
Identity = 915/1014 (90.24%), Postives = 948/1014 (93.49%), Query Frame = 0

Query: 62   MNQDGNTETVPPVQPSSLENQYIGDGNQSVATSTYLPLTSAPEAISWANHKVDGSSNENG 121
            MNQ GNTET  P QPSSLE Q+IGDGNQS ATSTYLPLT APEAI+WANHKVDGSSNENG
Sbjct: 1    MNQGGNTETFVPAQPSSLEIQHIGDGNQSAATSTYLPLTPAPEAITWANHKVDGSSNENG 60

Query: 122  LLPNSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSADPYGY 181
            LL NSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYA YSNS DPYGY
Sbjct: 61   LLSNSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAQYSNSTDPYGY 120

Query: 182  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQSLSSFQNTGFYAGSTSYSTTYYNPG 241
            ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQ LSS+QNTGFYAGSTSYSTTYYNPG
Sbjct: 121  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQPLSSYQNTGFYAGSTSYSTTYYNPG 180

Query: 242  DYQTAGGYQTSSYSNQTTSWNGGNYGNYVPNQYAQYTPDSSGAYSSSSTNANSLQYQQHY 301
            DYQTAGGY TSSYSNQTTSWNGGNYGNYVPNQYAQYTPDSSGAYSS+STNA+SLQYQQ  
Sbjct: 181  DYQTAGGYPTSSYSNQTTSWNGGNYGNYVPNQYAQYTPDSSGAYSSTSTNASSLQYQQQC 240

Query: 302  KQWADYYSQTEVSCAPGTEKLSTPGTANAGYPVHGSTSYPAPSSQ-PPPSYTPSWRPESS 361
            KQWADYYSQTEVSCAPGTEKLSTP  ANAGYP HGST+YPAP+SQ PPPSYTPSWRPES 
Sbjct: 241  KQWADYYSQTEVSCAPGTEKLSTPSIANAGYPAHGSTNYPAPNSQPPPPSYTPSWRPESG 300

Query: 362  SSELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLDLKTSYDSFQGQQKSA 421
            SSELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLDLK SYDSFQ QQKSA
Sbjct: 301  SSELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLDLKNSYDSFQDQQKSA 360

Query: 422  GPQGPNLQYPAHLAPQSYQLPSQSVPSLEARRTKLQIPTNPRIASNLSILKTSKDSSTVD 481
            GPQGPNLQYPAHLA QSYQLPSQSV  +EARRTKLQIPTNPRIASNLSILKTSKDSST D
Sbjct: 361  GPQGPNLQYPAHLASQSYQLPSQSVSPVEARRTKLQIPTNPRIASNLSILKTSKDSSTAD 420

Query: 482  AAVQPAYVSVSMPKPNEKELSNDTAEAVLKPGMFPKSLRGYVERALARCKDEKLMTSCQS 541
            A VQPAYVSVS+PK NEKELSNDT E+ LKPGMFPKSLRGYVERA+ARCKDEKLMTSCQS
Sbjct: 421  APVQPAYVSVSLPKLNEKELSNDTTESALKPGMFPKSLRGYVERAMARCKDEKLMTSCQS 480

Query: 542  VLKEMITKATADGTLYTKDWDIEPLFPLPSADAVNTEYYFSLAKLNNLQGSSPLSSLSKP 601
            VLKEMITKATADGTLYTKDWD+EPLFPLPSADAVNT+         NLQ  +P+SSLSK 
Sbjct: 481  VLKEMITKATADGTLYTKDWDVEPLFPLPSADAVNTD---------NLQVPTPISSLSKS 540

Query: 602  KRSPSRRSKSRWEPLPVEKPAEAHPPYSNGAAAKYDGWANVSERDKKTLSGNSETKNVVN 661
            +RSPSRRSKSRWEPLPVEKPAE  PP++NGAAAKY GWANVSER+KKTLSGNS+ K+V N
Sbjct: 541  RRSPSRRSKSRWEPLPVEKPAEPPPPHNNGAAAKYGGWANVSEREKKTLSGNSDMKDVSN 600

Query: 662  SRFPLWDQRTVGKISQGPAKKQRVADGSAPDNDGASSDSDKEQSLTAYYSGAMALANSPE 721
            SRFPLWDQRTVGKISQGPAKKQRVA+GS PDNDG SSDSDKEQSLTAYYSGAMALANSPE
Sbjct: 601  SRFPLWDQRTVGKISQGPAKKQRVANGSPPDNDGPSSDSDKEQSLTAYYSGAMALANSPE 660

Query: 722  EKKKRENRSKRFEKGHGHRGENNHFKPKNTGTGNLYTRRASALVLGKNLENGGCRAVEDI 781
            EKKKRENRSKRF+KGHGHRGENNHF+PKN G G+LYTRRASALV+GKNLENGG RAVEDI
Sbjct: 661  EKKKRENRSKRFDKGHGHRGENNHFRPKNAGIGSLYTRRASALVIGKNLENGGGRAVEDI 720

Query: 782  DWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVQMSQKNYLYKCDQLKSI 841
            DWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMV+MSQKNYLYKCDQLKSI
Sbjct: 721  DWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKALNMVKMSQKNYLYKCDQLKSI 780

Query: 842  RQDLTVQRIRNQLTAKVYEIHARLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYN 901
            RQDLTVQRIRNQLTAKVYE H RLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYN
Sbjct: 781  RQDLTVQRIRNQLTAKVYETHGRLALEVGDLPEYNQCQSQLKTLYAEGIEGCHMEFAAYN 840

Query: 902  LLCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRSAVTSGNYVKFFRLHKAAPNLN 961
            LLCAILHSNNKRDLLSLMSRLSDQAKKD AVNHALAVR+AVTS NYVKFFRL+KAAPNLN
Sbjct: 841  LLCAILHSNNKRDLLSLMSRLSDQAKKDAAVNHALAVRAAVTSENYVKFFRLYKAAPNLN 900

Query: 962  ACLMDLYAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGFFTSSG------DVDGLEECTEW 1021
            ACLMDLYAEKMRYKA+NCMSRSYRPSLPVPY+AQVLGF TS G      D DGLEECTEW
Sbjct: 901  ACLMDLYAEKMRYKAINCMSRSYRPSLPVPYVAQVLGFSTSFGDEVKDKDTDGLEECTEW 960

Query: 1022 LKGHGACLITDNNGEMQLDTKASSTTLYMPEPEDAVAHGDANLAVNDFFTRTSS 1069
            LK HGACLITD+NGEMQLD KASSTTLYMPEP+DAVAHGDANLAVNDFFTRTSS
Sbjct: 961  LKAHGACLITDSNGEMQLDAKASSTTLYMPEPDDAVAHGDANLAVNDFFTRTSS 1005

BLAST of Spg028591 vs. TAIR 10
Match: AT2G39340.1 (SAC3/GANP/Nin1/mts3/eIF-3 p25 family )

HSP 1 Score: 1021.1 bits (2639), Expect = 6.2e-298
Identity = 580/1032 (56.20%), Postives = 708/1032 (68.60%), Query Frame = 0

Query: 62   MNQDGNTETVPPVQPSSLENQYIGDGNQSVATSTYLPLTSAPEAISWANHKVDGSSNENG 121
            MN  GNT+ V P+ P+S+EN+Y  DG+Q+   S     ++  E+  W  H V+  + ENG
Sbjct: 1    MNHGGNTQAVAPMDPNSIENRYGVDGSQTQKYS--YQYSTGSESAPWTGHSVENQAVENG 60

Query: 122  LLPNSTYQYNQQVLPPARNVQDGLNVSSVACSSSSFVTSNAPQDYNAYAPYSNSADPYGY 181
               NS Y + Q   P   NVQ+  N  S   SS+S  T+N  QDY+ Y PY  S+DP+ Y
Sbjct: 61   NYSNSNYYHPQPTGPATGNVQEIPNTVSFTISSTS-GTANVAQDYSGYTPYQTSSDPHNY 120

Query: 182  ANAGYQGYYNNYQQQPNHSYSQPVGAYQNTGAPYQSLSSFQNTGFYAGSTSYSTTYYNPG 241
            +N GY  YY+ YQQQP+ SY QPVGAYQNTGAP Q LSSFQN G YAG+ SYS TYYNP 
Sbjct: 121  SNTGYSNYYSGYQQQPSQSYPQPVGAYQNTGAP-QPLSSFQNPGSYAGTPSYSGTYYNPA 180

Query: 242  DYQTAGGYQ-------------TSSYSNQTTSWNGGNYGNYVPNQYAQYTPDSSGAYSSS 301
            DYQTAGGYQ             +++YSNQT + N GNY +Y  N Y  YTPD++  +SS+
Sbjct: 181  DYQTAGGYQSTNYNNQTAGSYPSTNYSNQTPASNQGNYTDYTSNPYQNYTPDAANTHSST 240

Query: 302  STNANSLQYQQHYKQWADYYSQTEVSCAPGTEKLSTPGTA--NAGYPVHGSTS-YPAPSS 361
                  + YQQ+Y+QW +YYSQTEV CAPGTEKLSTP T+  +  +PV G TS  PA +S
Sbjct: 241  IATTPPVHYQQNYQQWTEYYSQTEVPCAPGTEKLSTPTTSAYSQSFPVPGVTSEMPASNS 300

Query: 362  QPPPSYTPSWRPESSSSELVSAQPGAVSSGNHDGYWKHGAPNSQVHLTNATQPHFEKPLD 421
            QP PSY   WRPE+ SS   S QPGA  S ++D YW H AP+ Q H     Q +++ PL+
Sbjct: 301  QPAPSYVQPWRPETDSSHPPSQQPGAAVSTSNDTYWMHQAPSLQAHHPVPPQNNYQSPLE 360

Query: 422  LKTSYDS-FQGQQKSAGPQGPNLQYPAHLAPQSYQLPSQSVPSLEARR-TKLQIPTNPRI 481
             K  Y++ FQG Q++  PQ  N Q   H AP  Y+ P+Q+ P ++++R +K+QIPTNPRI
Sbjct: 361  TKPLYETPFQGHQRATYPQEMNSQSSFHQAPLGYRQPTQTAPLVDSQRVSKVQIPTNPRI 420

Query: 482  ASNL--SILKTSKDSSTVDAAVQPAYVSVSMPKPNEKELSNDTAEAVLKPGMFPKSLRGY 541
            ASNL     K  KDS+   AA  PAYVSVSMPKP       D   A+  PG FPKSLRG+
Sbjct: 421  ASNLPSGFTKMDKDSTAASAAQAPAYVSVSMPKP------KDHTTAMSDPGTFPKSLRGF 480

Query: 542  VERALARCKDEKLMTSCQSVLKEMITKATADGTLYTKDWDIEPLFPLPSADAVNTEYYFS 601
            VERA ARCKD+K   SC+  L++++ KA  D TLYT+DWD EPL  + + +  N+E    
Sbjct: 481  VERAFARCKDDKEKESCEVALRKIVKKAKEDNTLYTRDWDTEPLSTVTTTNVTNSE---- 540

Query: 602  LAKLNNLQGSSPLSSLSKPKRSPSRRSKSRWEPLPVEKPAEAHPPYSNGAAAKYDGWANV 661
                     S+ LSSL    +SP+RR KSRWEPL   KP    P  +  +A K+  W + 
Sbjct: 541  -------SSSAQLSSLQ--NKSPTRRPKSRWEPLVEGKPF-VKPASTFSSAVKFGVWNHQ 600

Query: 662  SERDKKTLSGNSETKNVVNSRFPLWDQRTVGKISQGPAKKQRVADGSAPD-NDGASSDSD 721
            +E +KK+     +       +     Q +  K  Q P K+QR + G+A   +D ASSDSD
Sbjct: 601  NENNKKSSESFQKVDAATGFKPTYSGQNSAKKSFQRPVKRQRFSGGAATAIDDEASSDSD 660

Query: 722  KEQSLTAYYSGAMALANSPEEKKKRENRSKRFEKGHGHRGENNHFKPKNTGTGNLYTRRA 781
            K+  LT YYS AMALA S EEKK+R++RSKRFEK  GH   N+  KPKN   GNL++RRA
Sbjct: 661  KD--LTPYYSSAMALAGSAEEKKRRDSRSKRFEKIQGHSRGNDLTKPKNANVGNLHSRRA 720

Query: 782  SALVLGKNLENGGCRAVEDIDWDALTIKGTCQEIEKRYLRLTSAPDPSSVRPEEVLEKAL 841
            +AL L K  +  G RAVEDIDWDALT+KGTCQEIEKRYLRLTSAPDP++VRPE+VLEKAL
Sbjct: 721  TALRLSKVFDESGSRAVEDIDWDALTVKGTCQEIEKRYLRLTSAPDPATVRPEDVLEKAL 780

Query: 842  NMVQMSQKNYLYKCDQLKSIRQDLTVQRIRNQLTAKVYEIHARLALEVGDLPEYNQCQSQ 901
             MVQ SQKNYL+KCDQLKSIRQDLTVQRI N LTAKVYE HARLALE GDLPEYNQC SQ
Sbjct: 781  IMVQDSQKNYLFKCDQLKSIRQDLTVQRIHNHLTAKVYETHARLALEAGDLPEYNQCLSQ 840

Query: 902  LKTLYAEGIEGCHMEFAAYNLLCAILHSNNKRDLLSLMSRLSDQAKKDVAVNHALAVRSA 961
            LKTLYAEG+EGC +EFAAY+LL   LHSNN R+LLS MSRLS++ KKD AV HAL+VR+A
Sbjct: 841  LKTLYAEGVEGCSLEFAAYSLLYITLHSNNNRELLSSMSRLSEEDKKDEAVRHALSVRAA 900

Query: 962  VTSGNYVKFFRLHKAAPNLNACLMDLYAEKMRYKAVNCMSRSYRPSLPVPYIAQVLGFF- 1021
            VTSGNYV FFRL+K APN+N+CLMDLY EKMRYKAVN MSRS RP++PV YI QVLGF  
Sbjct: 901  VTSGNYVMFFRLYKTAPNMNSCLMDLYVEKMRYKAVNFMSRSCRPTIPVSYIVQVLGFTG 960

Query: 1022 -----TSSGDVDGLEECTEWLKGHGACLITDNNGEMQLDTKASSTTLYMPEPEDAVAHGD 1067
                 T   + DG+E+C EWLK HGA +ITD+NG+M LDTKA+ST+L+MPEPEDAVAHGD
Sbjct: 961  AASEGTDEKETDGMEDCLEWLKTHGANIITDSNGDMLLDTKATSTSLFMPEPEDAVAHGD 1006

BLAST of Spg028591 vs. TAIR 10
Match: AT1G28060.1 (Pre-mRNA-splicing factor 3 )

HSP 1 Score: 46.2 bits (108), Expect = 1.9e-04
Identity = 21/34 (61.76%), Postives = 28/34 (82.35%), Query Frame = 0

Query: 16  KLSVNAWENRLTGCAVICDGISVLVVEGGGKSIK 50
           K+ +NA ENRLTGC+V+ D +SV+VVEG  K+IK
Sbjct: 670 KVEMNARENRLTGCSVMTDEMSVVVVEGKSKAIK 703